Cell program cooling instrument with code scanning function

Abstract

The application discloses a cell program cooling instrument with a code scanning function, which comprises a code scanning module, a temperature control module and a central processing module. The code scanning module reads the bar code or two-dimensional code information of a sample container. The temperature control module adjusts the cooling rate and temperature according to a preset program. The central processing module is in communication connection with the first two modules and is used for data receiving, processing, display and storage. The code scanning module adopts a camera scanning module. The temperature control module is embedded with a distributed temperature sensor array, and the temperature control is accurate. The central processing module comprises a storage module, a data processing module and a display module, supports data export, remote backup and split use. The application also discloses a sample data association method and a management method, realizes integrated management and whole life cycle tracing of sample information and cooling operation data, solves the problems of insufficient temperature control precision of traditional equipment, difficulty in sample tracing and the like, improves the cell recovery rate and meets the sample whole process control requirement.

Description

Technical Field

[0001] This invention relates to the field of biomedical and pharmaceutical production equipment technology, specifically to a cell programmed cooling device with barcode scanning function. Background Technology

[0002] In the biomedical field, cell preservation requires strict temperature control procedures to ensure that cell viability and function are not impaired. In the pharmaceutical manufacturing field, full traceability management is also necessary throughout the entire process of cell production, preservation, and transportation.

[0003] Traditional cell temperature-programmed cooling systems typically only have basic temperature control functions, lacking the ability to precisely control the cooling rate and effectively record and trace the operation process. This poses certain inconveniences for the management of cell samples, especially for cell-based drugs, particularly in situations requiring traceability management of frozen and stored samples.

[0004] Traditional equipment suffers from the following technical defects: First, insufficient temperature control precision leads to a large deviation between the sample cooling rate and the set value, resulting in a low recovery rate for some cells; second, sample traceability is difficult; third, the lack of data storage and traceability mechanisms makes it difficult to achieve full-process control of the cell freezing process, posing a threat to the safety and reliability of cell samples; fourth, the traditional method of writing on sample containers manually is difficult because the containers are mostly small cylinders, making it hard to write neatly, resulting in illegible handwriting; furthermore, the high humidity of the storage environment can cause the ink to fade and spread, eventually becoming unreadable. Summary of the Invention

[0005] In view of the shortcomings of the existing technology, the purpose of this invention is to provide a cell programmed cooling device with barcode scanning function to solve the problems mentioned in the background art.

[0006] According to one aspect of this application, a cell programmed cooling device with barcode scanning function is characterized by comprising a barcode scanning module, a temperature control module, and a central processing module. The barcode scanning module is used to read the barcode or QR code information of the sample container; The temperature control module is used to adjust the cooling rate and temperature of the equipment according to a preset program; The central processing module is connected to both the barcode scanning module and the temperature control module. It receives operating data from the temperature control module, processes data uploaded by the barcode scanning module, displays the cooling curve, barcode scanning results, and stores data summaries. It also stores temperature data and sample information in real time.

[0007] Preferably, the scanning module includes a scanning terminal and a scanning control circuit. The scanning terminal is used to read barcode or QR code information on the surface of the sample container, and the scanning control circuit is used to process the signal read by the scanning terminal and convert it into identifiable data before uploading it to the central processing module.

[0008] Preferably, the temperature control module includes a control circuit board, a cooling element, and a metal base. The metal base is fitted to the cold head of the cooling element, and a distributed temperature sensor array is embedded in the metal base. The metal base is capable of holding a sample container.

[0009] Preferably, the central processing module includes a storage module, which is used to store temperature data collected during instrument operation and sample information corresponding to the barcode scanning module in real time. The storage module supports data export and remote backup functions.

[0010] Preferably, the central processing module includes a data processing module, which is used to interact with the user, control the operation of the temperature control module, receive operating data fed back by the temperature control module, and process data uploaded by the barcode scanning module.

[0011] Preferably, the central processing module includes a display module, which is used to set and display the cooling curve, barcode scanning results and stored data summary, and provides an input interface for configuring control parameters. The display module can be used in combination with the cell programmable cooling device or separately from the cell programmable cooling device.

[0012] Preferably, the display module includes a touch screen, which is used to interact with the user to set parameters for the freezing process and view the device's operating curve and data from the barcode scanning module.

[0013] Preferably, it also includes a power module, which provides power to the barcode scanning module, the temperature control module, and the central processing module.

[0014] A method for linking sample data includes the following steps: Step 1: Scan the barcode or QR code on the surface of the sample container using the scanning module of the cell programmable cooling instrument, read the original identification information of the sample container, and upload the original identification information to the central processing module. Step 2: The central processing module generates a globally unique sample association identifier based on the original identification information, combined with the device's unique identifier and timestamp, to ensure the uniqueness of the identifier during the device's operating cycle. Step 3: The central processing module sends a cooling program start command to the temperature control module, and at the same time establishes a synchronization clock based on the timestamp to ensure the consistency of the time between the start of the cooling program and the data association. Step 4: The temperature control module operates according to the preset program, collecting real-time temperature data, cooling rate data and operating status parameters through a distributed temperature sensor array, and feeding the above data back to the central processing module in real time. Step 5: The central processing module matches and binds the received temperature-related data with the sample association identifier one by one according to the timestamp, forming an association data chain of identifier-time-temperature parameter, and at the same time as the original identification information of the sample container. Step Six: The central processing module stores the associated data chain to the storage module in real time, supports local encrypted storage of data, and archives the data by identifier. It also ensures the synchronization between the data storage process and the cooling program, providing complete data support for subsequent sample tracing and data retrieval.

[0015] A sample data management method includes the following steps: The barcode or QR code on the surface of the biological sample container is scanned by the barcode scanning module of the cell program cooling instrument to obtain the unique number information and preset associated identifier of the sample container. The central processing module of the cell-programmed cooling instrument automatically associates and matches the unique identification information, preset association identifiers, and pre-stored basic sample information and historical processing data. The basic sample information includes the sample name, source, storage requirements, and processing batch. The historical processing data includes previous cooling operation records and status change information. During the sample storage phase, the storage location information is entered synchronously and bound to the aforementioned associated data to form a complete sample information archive, which is then stored in the storage module. During the sample retrieval stage, the barcode or QR code of the sample container is scanned again by the scanning module to retrieve the corresponding full sample information file, record the retrieval time, retrieval purpose and post-retrieval status, and update the sample status data in the storage module in real time. During the sample retrieval phase, it supports accurate retrieval and information retrieval through the display module or data export function of the central processing module based on any one or more of the following conditions: the unique number of the sample container, the preset association identifier, the basic information of the sample, or the historical processing data. Through the above process, the entire process of biological sample data linkage is realized from storage and entry, retrieval and traceability to retrieval and query, achieving integrated management and full life cycle traceability of sample information and cooling device operation data.

[0016] The advantages of this application compared to existing technologies are as follows: the scanning module enables rapid reading of sample container identification information, avoiding the identification difficulties caused by manually written labels; the temperature control module improves the cooling rate and temperature control accuracy through a distributed temperature sensor array and precise temperature control logic, thereby increasing the cell recovery rate; the central processing module enables real-time association and storage of temperature data and sample information, supports data export, remote backup and precise retrieval, establishes a full life cycle traceability system for samples, solves the problem of the lack of data storage and traceability mechanisms in traditional equipment, and meets the stringent requirements for cell sample management in the biomedical and pharmaceutical production fields. Attached Figure Description

[0017] Figure 1 This is a topological schematic diagram of a cell programmed cooling device with barcode scanning function according to an embodiment of this application.

[0018] Figure 2 This is a schematic diagram of the external structure of a cell programmed cooling device with barcode scanning function according to an embodiment of this application.

[0019] Figure 3 This is a schematic diagram of the cooling element and metal base of a cell programmed cooling device with barcode scanning function according to an embodiment of this application.

[0020] Figure 4 This is a graph showing the results of the comparative analysis of the resuscitation activity of induced pluripotent stem cells in Example 4.

[0021] Attached reference numerals: 1. Cooling device; 2. Barcode scanning module; 3. Central processing module; 4. Metal base; 5. Refrigeration element. Detailed Implementation

[0022] To make the content of this application easier to understand, the appendices in the embodiments of this application will be described below. Figure 1-3 The technical solutions in the embodiments of this application will be clearly and completely described.

[0023] Example 1 like Figures 1-3 As shown, a cell programmed cooling device 1 with barcode scanning function includes a barcode scanning module 2, a temperature control module, and a central processing module 3.

[0024] The scanning module 2 is used to read the barcode and QR code information of the sample container. The scanning module 2 includes a scanning terminal and a scanning control circuit. The scanning terminal reads the barcode or QR code information on the surface of the sample container, and the scanning control circuit processes the signal read by the scanning terminal and converts it into identifiable data before uploading it to the central processing module 3. The barcodes or QR codes on the sample containers are mostly made using laser etching, which is resistant to low-temperature environments, maintains clarity for a long time, and is easy to identify. Scanning allows for the rapid acquisition of the sample tube number and automatic retrieval of other sample information, further enabling the management of samples entering and leaving refrigerators or liquid nitrogen tanks.

[0025] The temperature control module is used to adjust the cooling rate and temperature value according to a preset program. The temperature control module includes a control circuit board, a cooling element 5, and a metal base 4. The metal base 4 is fitted to the cold head of the cooling element 5. The metal base 4 embeds a distributed temperature sensor array for accurate temperature data acquisition. The metal base 4 also has matching slots for placing sample containers. The control circuit board executes the cooling program for the cell samples, including setting and controlling parameters such as the cooling rate and target temperature. The cooling element 5 can be a refrigerator; in this example, a Stirling refrigerator is preferred to provide a cold source with a cooling capacity below -80℃. The central processing module 3 is communicatively connected to both the barcode scanning module 2 and the temperature control module. It receives operating data from the temperature control module, processes data uploaded by the barcode scanning module 2, displays the cooling curve, scanning results, and stores data summaries, and stores temperature data and sample information in real time.

[0026] The central processing module 3 integrates a storage module, a data processing module, and a display module. The storage module stores temperature data collected during instrument operation and sample information corresponding to the barcode scanning module 2 in real time. The storage module supports data export and remote backup. The data processing module handles user interaction, controls the operation of the temperature control module, receives operational data from the temperature control module, and processes data uploaded by the barcode scanning module 2. The display module sets and displays the cooling curve, barcode scanning results, and stored data summaries, and provides an input interface for configuring control parameters. Furthermore, the display module can be used in conjunction with the cell programmable refrigeration instrument 1 or separately. The display module includes a touchscreen for user interaction, allowing users to set parameters for the programmed freezing process and view the equipment's operating curves and data from the barcode scanning module 2. The temperature control module compensates for thermal inertia using a dynamic PID algorithm and combines it with a distributed temperature sensor array to improve linear cooling accuracy and rate.

[0027] The cell-programmed cooling device 1 also includes a power module for providing power to the barcode scanning module 2, the temperature control module, and the central processing module 3.

[0028] Example 2 A method for linking sample data includes the following steps: Step 1: Scan the barcode or QR code on the surface of the sample container using the barcode scanning module 2 of the cell program cooling instrument 1, read the original identification information of the sample container, and upload the original identification information to the central processing module 3. Step 2: Based on the original identification information, the central processing module 3 generates a globally unique sample association identifier by combining the device's unique identifier and timestamp, ensuring the uniqueness of this identifier within the device's operating cycle. Step 3: The central processing module 3 sends a cooling program start command to the temperature control module, and at the same time establishes a synchronization clock based on the timestamp to ensure the consistency of the time between the start of the cooling program and the data association. Step 4: The temperature control module operates according to the preset program, collecting real-time temperature data, cooling rate data and operating status parameters through a distributed temperature sensor array, and feeding the above data back to the central processing module 3 in real time. Step 5: The central processing module 3 matches and binds the received temperature-related data with the sample association identifier one by one according to the timestamp, forming an association data chain of identifier-time-temperature parameter, and at the same time as the original identification information of the sample container. Step 6: The central processing module 3 stores the associated data chain to the storage module in real time, supports local encrypted storage of data, and archives the data by identifier. It also ensures the synchronization between the data storage process and the cooling program, providing complete data support for subsequent sample tracing and data retrieval.

[0029] Example 3 A sample data management method includes the following steps: The barcode or QR code on the surface of the biological sample container is scanned by the barcode scanning module 2 of the cell program cooling instrument 1 to obtain the unique number information and preset associated identifier of the sample container. The central processing module 3 of the cell program cooling instrument 1 automatically associates and matches the unique number information, preset association identifier, and pre-stored sample basic information and historical processing data. The sample basic information includes sample name, source, storage requirements, and processing batch. The historical processing data includes previous cooling operation records and status change information. During the sample storage phase, the storage location information is entered synchronously and bound to the aforementioned associated data to form a complete sample information archive, which is then stored in the storage module. During the sample retrieval stage, the barcode or QR code of the sample container is scanned again by the scanning module 2 to retrieve the corresponding full sample information file, record the retrieval time, retrieval purpose and post-retrieval status, and update the sample status data in the storage module in real time. During the sample retrieval stage, it supports accurate retrieval and information retrieval based on any one or more of the following conditions: the unique number of the sample container, the preset association identifier, the basic information of the sample, or the historical processing data, through the display module or data export function of the central processing module 3. Through the above process, the entire process of biological sample data linkage from storage and entry, retrieval and traceability to retrieval and query is realized, achieving integrated management and full life cycle traceability of sample information and cooling device 1 operation data.

[0030] Example 4 Application Example: Comparison of the resuscitation activity of induced pluripotent stem cells 1. Cell type selection: Induced pluripotent stem cells; 2. Cryopreservation preservative: 10% ethylene glycol; 3. Treatment method for experimental group 1: Induced pluripotent stem cells were added to 10% ethylene glycol cryopreservative, placed in a programmed cooling box, and stored overnight at -80°C. After that, they were placed in liquid nitrogen. 4. Treatment method for experimental group 2: Induced pluripotent stem cells were added to 10% ethylene glycol cryopreservative and then subjected to the following cooling steps: 1) Cooling from 0℃ to -10℃ / min, with a cooling rate of -1℃ / min; 2) Keep warm at -10℃ for 30 minutes; 3) Cooling from -10℃ to -40℃ at a rate of -3℃ / min; 4) Cooling from -40℃ to -60℃ at a rate of -1℃ / min; 5) Cooling from -60℃ to -80℃ at a rate of -0.33℃ / min; 6) Keep warm at -80℃ for 5 minutes, then transfer to a liquid nitrogen tank.

[0031] 5. Detection method: After placing the two groups of samples in liquid nitrogen for three days, they were taken out for revival, cultured for three days, and the cell count was measured. The revival rate was calculated by comparing the samples with those without cryopreservation treatment. 6. Test Results: Table 1

[0032] This application example demonstrates that using the cell programmed cooling device 1 of the present invention for precise temperature control can significantly improve the recovery rate of induced pluripotent stem cells, reflecting the accuracy and practicality of the device's temperature control.

[0033] The above embodiments are only used to illustrate the technical solutions of the embodiments of this application, and are not intended to limit them. Although the embodiments of this application have been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features, without departing from the spirit and scope defined by the claims of this application.

Claims

1. A cell programmed cooling device with barcode scanning function, characterized in that, It includes a barcode scanning module, a temperature control module, and a central processing module; The barcode scanning module is used to read the barcode or QR code information of the sample container; The temperature control module is used to adjust the cooling rate and temperature of the equipment according to a preset program; The central processing module is connected to both the barcode scanning module and the temperature control module. It controls the operation of the temperature control module, receives operating data from the temperature control module, processes data uploaded by the barcode scanning module, displays the cooling curve, barcode scanning results, and stores data summaries, and stores temperature data and sample information in real time.

2. The cell programmed cooling device according to claim 1, characterized in that, The scanning module includes a scanning terminal and a scanning control circuit. The scanning terminal is used to read barcode or QR code information on the surface of the sample container. The scanning control circuit is used to process the signal read by the scanning terminal and convert it into identifiable data, which is then uploaded to the central processing module.

3. The cell programmed cooling device according to claim 1, characterized in that, The temperature control module includes a control circuit board, a cooling element, and a metal base. The metal base is fitted to the cold head of the cooling element. A distributed temperature sensor array is embedded in the metal base. The metal base can hold a sample container.

4. The cell programmed cooling device according to claim 1, characterized in that, The central processing module includes a storage module, which is used to store temperature data collected during instrument operation and sample information corresponding to the barcode scanning module in real time. The storage module supports data export and remote backup functions.

5. The cell programmed cooling device according to claim 1, characterized in that, The central processing module includes a data processing module, which is used to interact with the user, control the operation of the temperature control module, receive operating data fed back by the temperature control module, and process data uploaded by the barcode scanning module.

6. The cell programmed cooling device according to claim 1, characterized in that, The central processing module includes a display module, which is used to set and display the cooling curve, barcode scanning results and stored data summary, and provides an input interface for configuring control parameters. The display module can be used in combination with the cell programmable cooling device or separately from the cell programmable cooling device.

7. The cell programmed cooling device according to claim 6, characterized in that, The display module includes a touch screen, which is used to interact with the user to set parameters for the freezing process and view the device's operating curve and data from the barcode scanning module.

8. The cell programmed cooling device according to claim 1, characterized in that, It also includes a power module, which provides power to the barcode scanning module, the temperature control module and the central processing module.

9. A sample data association method, applied to the cell programmed cooling device according to any one of claims 1-8, characterized in that, Includes the following steps: Step 1: Scan the barcode or QR code on the surface of the sample container using the scanning module of the cell programmable cooling instrument, read the original identification information of the sample container, and upload the original identification information to the central processing module. Step 2: The central processing module generates a globally unique sample association identifier based on the original identification information, combined with the device's unique identifier and timestamp, to ensure the uniqueness of the identifier during the device's operating cycle. Step 3: The central processing module sends a cooling program start command to the temperature control module, and at the same time establishes a synchronization clock based on the timestamp to ensure the consistency of the time between the start of the cooling program and the data association. Step 4: The temperature control module operates according to the preset program, collecting real-time temperature data, cooling rate data and operating status parameters through a distributed temperature sensor array, and feeding the above data back to the central processing module in real time. Step 5: The central processing module matches and binds the received temperature-related data with the sample association identifier one by one according to the timestamp, forming an association data chain of identifier-time-temperature parameter, and at the same time as the original identification information of the sample container. Step Six: The central processing module stores the associated data chain to the storage module in real time, supports local encrypted storage of data, and archives the data by identifier. It also ensures the synchronization between the data storage process and the cooling program, providing complete data support for subsequent sample tracing and data retrieval.

10. A sample data management method, applied to the cell programmed cooling instrument according to any one of claims 1-8, characterized in that, Includes the following steps: The barcode or QR code on the surface of the biological sample container is scanned by the barcode scanning module of the cell program cooling instrument to obtain the unique number information and preset associated identifier of the sample container. The central processing module of the cell-programmed cooling instrument automatically associates and matches the unique identification information, preset association identifiers, and pre-stored basic sample information and historical processing data. The basic sample information includes the sample name, source, storage requirements, and processing batch. The historical processing data includes previous cooling operation records and status change information. During the sample storage phase, the storage location information is entered synchronously and bound to the aforementioned associated data to form a complete sample information archive, which is then stored in the storage module. During the sample retrieval stage, the barcode or QR code of the sample container is scanned again by the scanning module to retrieve the corresponding full sample information file, record the retrieval time, retrieval purpose and post-retrieval status, and update the sample status data in the storage module in real time. During the sample retrieval phase, it supports accurate retrieval and information retrieval through the display module or data export function of the central processing module based on any one or more of the following conditions: the unique number of the sample container, the preset association identifier, the basic information of the sample, or the historical processing data. Through the above process, the entire process of biological sample data linkage is realized from storage and entry, retrieval and traceability to retrieval and query, achieving integrated management and full life cycle traceability of sample information and cooling device operation data.

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