A fresh-keeping agent for promoting the opening of cut peony flowers and application thereof

Abstract

The present application belongs to the field of plant growth regulation, and provides a fresh-keeping agent for promoting the opening of cut peony flowers, wherein the fresh-keeping agent is an aqueous solution of methyl jasmonate; the concentration of the methyl jasmonate is 25-100 μmol / L. The present application also provides a method for promoting the opening of cut peony flowers, which comprises the operation of culturing peony flowers with an aqueous solution of methyl jasmonate, and the concentration of the aqueous solution of methyl jasmonate is 25-100 μmol / L. By treating the cut peony flowers with the aqueous solution of methyl jasmonate, the present application can effectively solve the problem of difficult flowering of the cut peony flowers in the tight bud stage or just entering the loose bud stage, promote the flowering of the cut peony flowers, and improve the complete opening rate of the flower buds.

Description

Technical Field

[0001] This invention belongs to the field of plant growth regulation technology, specifically relating to a preservative that promotes the opening of cut peony flowers and its application. Background Technology

[0002] Peony ( Paeonia sect. Paeonia Peonies, a traditional flower with thousands of years of cultivation history, possess significant ornamental and cultural value, and the domestic market for cut peonies has been growing year by year. In the industrial production of cut peonies, to facilitate long-distance transportation, reduce mechanical damage, and extend shelf life, harvesting is usually carried out in the early stages of bud development, when the petals of the peony buds are partially exposed and beginning to soften. However, while this harvesting method solves logistical problems, it brings about a common product issue: a significant portion of cut flowers are harvested while still in a firm bud stage (commonly known as "iron eggs" or "raw cut flowers"). Because the buds detach from the parent plant too early, coupled with insufficient accumulated nutrients and flowering-related hormone signals, many cut flowers fail to open properly during vase arrangement. This manifests as petals not fully extending, only the outer petals barely opening while the inner petals remain tightly closed, and browning of the petals and gradual death of the buds, severely impacting their ornamental value and resulting in a low yield of high-quality products. Compared to other flowers, the problem of "iron eggs not blooming" in peonies is particularly prominent.

[0003] Currently, most flower preservatives on the market focus on sterilization and providing nutrients, with no products available to regulate the internal development of flower buds or promote flowering. Existing technologies lack a dedicated preservation formula that can effectively address the flowering challenges of cut peonies in their bud-firming or just-entering-softening stages, while simultaneously delaying aging. Therefore, developing a technology that significantly promotes the normal opening of cut peonies is a pressing problem in this field. Summary of the Invention

[0004] To address the aforementioned problems in the prior art, this invention provides a preservative for promoting the opening of cut peony flowers and its application.

[0005] This invention provides a preservative that promotes the opening of cut peony flowers, wherein the preservative contains methyl jasmonate.

[0006] The present invention also provides a method for promoting the opening of cut peony flowers, the method comprising treating the peony with methyl jasmonate.

[0007] Compared with the prior art, the present invention has the following beneficial effects:

[0008] This invention treats cut peonies with an aqueous solution of methyl jasmonate, which can significantly increase the proportion of cut peonies that are in the bud-firming stage or just entering the bud-softening stage and open normally during vase life. It effectively solves the problem of difficult flowering of cut peonies in the bud-firming stage or just entering the bud-softening stage, promotes flowering of cut peonies, and increases the rate of complete bud opening.

[0009] The preservative of the present invention is inexpensive, easy to use, and easy to promote and use. Attached Figure Description

[0010] Figure 1 These are photographs of the open buds of 'Sarah' in the control group and treatment group 3 in Example 1.

[0011] Figure 2 These are photographs of the 'Laura's Dessert' flower buds in the control group and treatment group 3 of Example 1, showing their open state. Detailed Implementation

[0012] To make the technical solution, objectives, and advantages of the present invention clearer, the present invention will be further described in detail below through specific embodiments. It should be understood that the specific embodiments described herein are for illustration and explanation only and are not intended to limit the present invention.

[0013] In a first aspect, the present invention provides a preservative that promotes the opening of cut peony flowers, the preservative containing methyl jasmonate (MeJA).

[0014] According to a first aspect of the present invention, the preservative may be an aqueous solution of methyl jasmonate.

[0015] In the aqueous solution of methyl jasmonate, the concentration of methyl jasmonate is 25~100 μmol / L (μM), for example, it can be any one or a range between two of 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 and 100 μmol / L, for example, any one of 70~80 μmol / L, 65~85 μmol / L, 50~80 μmol / L, 45~90 μmol / L, preferably 75 μmol / L.

[0016] According to a first aspect of the present invention, the preservative may further contain a basic preservative solution, the basic preservative solution containing:

[0017] 8-hydroxyquinoline with a final concentration of 50-250 mg / L, preferably 150 mg / L;

[0018] Citric acid with a final concentration of 50-150 mg / L, preferably 100 mg / L;

[0019] The sucrose has a final concentration of 20-50 g / L, preferably 30-50 g / L, and more preferably 40 g / L.

[0020] In a preferred embodiment, the method for preparing the preservative includes:

[0021] According to the above concentration requirements, weigh out the mother liquor of methyl jasmonate and 8-hydroxyquinoline, citric acid, and sucrose, dissolve them in an appropriate amount of water and mix well to obtain the preservative.

[0022] The mother liquor of the above-mentioned 8-hydroxyquinoline can be an ethanol solution of 8-hydroxyquinoline with a concentration of 30 g / L.

[0023] Secondly, the present invention provides a method for promoting the opening of cut peony flowers, the method comprising treating the peony with methyl jasmonate.

[0024] According to a second aspect of the present invention, the operation of treating peony with methyl jasmonate includes culturing peony with an aqueous solution of methyl jasmonate.

[0025] In the aqueous solution of methyl jasmonate, the concentration of methyl jasmonate is 25~100 μmol / L (μM), preferably 75 μmol / L.

[0026] According to a second aspect of the present invention, the aqueous solution of methyl jasmonate may further contain a basic preservative solution, said basic preservative solution containing:

[0027] 8-hydroxyquinoline with a final concentration of 50-250 mg / L, preferably 150 mg / L;

[0028] Citric acid with a final concentration of 50-150 mg / L, preferably 100 mg / L;

[0029] The sucrose has a final concentration of 20-50 g / L, preferably 30-50 g / L, and more preferably 40 g / L.

[0030] Thirdly, the present invention provides the application of methyl jasmonate in promoting the opening of cut peony flowers.

[0031] Fourthly, the present invention provides the use of methyl jasmonate in the preparation of formulations for promoting the opening of cut peony flowers.

[0032] Unless otherwise specified, all reagents and materials used in the following examples are products that can be obtained from commercial channels; unless otherwise specified, all testing and detection methods used in the following examples are conventional testing and detection methods in the field and can be obtained from textbooks, reference books or academic journals.

[0033] Example 1

[0034] This example illustrates the application of a preservative containing methyl jasmonate (MeJA) in promoting the opening (flowering) of cut peony flowers during the bud compaction stage.

[0035] 1. Materials and Methods

[0036] 1.1 Experimental Materials

[0037] Five-year-old, robust peony varieties 'Sarah' and 'Laura Dessert' were selected as experimental materials. The plants were sourced from the Peony Germplasm Resource Nursery of the Institute of Grassland, Flower and Landscape Ecology, Beijing Academy of Agricultural and Forestry Sciences, Tel: 010-81127866.

[0038] Basic preservative solution: contains 150 mg / L 8-hydroxyquinoline, 100 mg / L citric acid, and 40 g / L sucrose.

[0039] MeJA concentration gradient solutions: Based on the above basic preservation solution, MeJA was added to final concentrations of 25 μmol / L, 50 μmol / L, 75 μmol / L, and 100 μmol / L, respectively.

[0040] Solvent: Deionized water.

[0041] 1.2 Experimental Methods

[0042] During the bud formation stage, the flower buds, along with their stems (20cm), were cut off and inserted into test tubes of different experimental groups. These tubes were then placed in a plant incubator at 26℃ during the light period (16 h) and 22℃ during the dark period (8 h), with a light intensity of 20000 lux, to observe and record the flowering status.

[0043] Control group: Basic preservative solution (without MeJA);

[0044] Treatment group 1: Basic preservative solution + 25 μmol / L MeJA;

[0045] Treatment group 2: Basic preservative solution + 50 μmol / L MeJA;

[0046] Treatment group 3: Basic preservative solution + 75 μmol / L MeJA;

[0047] Treatment group 4: Basic preservative solution + 100 μmol / L MeJA.

[0048] Each experimental group had 20 samples, and the openness of the samples was observed and recorded at the following 6 time points: Day 1 (the day the test tube was inserted), Day 2, Day 3, Day 5, Day 7, and Day 9; the above experiment was repeated three times.

[0049] 1.3 Morphological observation and statistical analysis

[0050] The development of flower buds and flowering performance of each experimental group were monitored, and the samples of each group were photographed and recorded. The data from the three repeated experiments were statistically analyzed.

[0051] 2. Experimental Results

[0052] 2.1 Statistical analysis of the opening of 'Sarah' flower buds cut from different experimental groups

[0053] The results are as follows Figure 1 As shown in Table 1. The results showed that, up to day 9, the fully open rate (i.e., the percentage of fully open buds to the total number of buds; in this invention, "fully open" means that all petals are open and the stamen in the center of the bud is exposed) of the cut 'Sarah' buds in the control group was 0%, the fully open rate of treatment group 1 (25 μmol / L MeJA) was 13.3%, the fully open rate of treatment group 2 (50 μmol / L MeJA) was 33.3%, the fully open rate of treatment group 3 (75 μmol / L MeJA) was 76.7%, and the fully open rate of treatment group 4 (100 μmol / L MeJA) was 40%.

[0054] Among the treatments, the longest time required for full opening was for treatment group 1 (25 μmol / L MeJA), followed by treatment group 2 (50 μmol / L MeJA), while treatment groups 3 (75 μmol / L MeJA) and 4 (100 μmol / L MeJA) had the shortest, with an average of 2.3 days. There was no significant difference in the number of days to maintain openness among the four treatments, all around 5 days. Overall, treatment group 3 (75 μmol / L MeJA) was the most suitable concentration for promoting the opening of cut flower buds in the 'Sarah' variety.

[0055] Table 1: Statistics on the opening data of 'Sarah' flower buds in different experimental groups

[0056]

[0057] 2.2 Statistical analysis of the opening of 'Laura's Dessert' flower buds cut from different experimental groups

[0058] The results are as follows Figure 2As shown in Table 2. The results showed that by day 9, only the outermost petals of the 'Laura's Dessert' buds in the control group had opened, while the inner petals showed only signs of loosening and were not fully open. Therefore, the full opening rate (i.e., the percentage of fully open buds to the total number of buds) was 0%. In contrast, the full opening rate of treatment group 1 (25 μmol / L MeJA) was 10%, treatment group 2 (50 μmol / L MeJA) was 43.3%, treatment group 3 (75 μmol / L MeJA) was 83.3%, and treatment group 4 (100 μmol / L MeJA) was 36.7%.

[0059] Among the treatments, the longest time required for full opening was for treatment group 1 (25 μmol / L MeJA), followed by treatment group 2 (50 μmol / L MeJA), while treatment groups 3 (75 μmol / L MeJA) and 4 (100 μmol / L MeJA) had the shortest, with an average of 2.3 days. There was no significant difference in the number of days to maintain openness among the four treatments, all around 5 days. Overall, treatment group 3 (75 μmol / L MeJA) was the most suitable concentration for promoting the opening of cut flower buds in the 'Laura Dessert' variety.

[0060] Table 2: Statistics on the opening data of 'Laura's Dessert' flower buds in different experimental groups

[0061]

[0062] In summary, this embodiment focuses on the research of cut buds from two mainstream cut peony varieties, 'Sarah' and 'Laura Dessert'. Based on basic flower preservatives, the addition of MeJA in the treatment experiment verified that MeJA has a regulatory function in promoting the opening of cut peony buds, providing a breakthrough solution to the important industry problem of the inability of cut peony buds to open in cut peony production. Furthermore, in the industry, buds on the lateral branches of multi-headed cut flower products are usually in the bud-firming stage or just entering the softening stage at harvest, making them difficult to open. Theoretically, the formulation of this invention should also promote the flowering of the aforementioned multi-headed cut peony products.

[0063] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A preservative that promotes the opening of cut peony flowers, characterized in that: The preservative contains methyl jasmonate.

2. The preservative for promoting the opening of cut peony flowers according to claim 1, characterized in that: The preservative is an aqueous solution of methyl jasmonate; The concentration of methyl jasmonate is 25~100 μmol / L.

3. The preservative for promoting the opening of cut peony flowers according to claim 2, characterized in that: The concentration of methyl jasmonate was 75 μmol / L.

4. The preservative for promoting the opening of cut peony flowers according to any one of claims 1-3, characterized in that: The preservative also contains a basic preservative solution, which contains: 8-hydroxyquinoline with a final concentration of 50-250 mg / L, preferably 150 mg / L; Citric acid with a final concentration of 50-150 mg / L, preferably 100 mg / L; The sucrose has a final concentration of 20-50 g / L, preferably 30-50 g / L, and more preferably 40 g / L.

5. A method for promoting the opening of cut peony flowers, characterized in that: The method includes treating peony with methyl jasmonate.

6. The method for promoting the opening of cut peony flowers according to claim 5, characterized in that: The operation of treating peony with methyl jasmonate includes culturing peony with an aqueous solution of methyl jasmonate. The concentration of methyl jasmonate aqueous solution is 25~100 μmol / L.

7. The method for promoting the opening of cut peony flowers according to claim 6, characterized in that: The concentration of methyl jasmonate aqueous solution is 75 μmol / L.

8. The method for promoting the opening of cut peony flowers according to any one of claims 5-7, characterized in that: The aqueous solution of methyl jasmonate also contains a basic preservative solution, which contains: 8-hydroxyquinoline with a final concentration of 50-250 mg / L, preferably 150 mg / L; Citric acid with a final concentration of 50-150 mg / L, preferably 100 mg / L; The sucrose has a final concentration of 20-50 g / L, preferably 30-50 g / L, and more preferably 40 g / L.

9. Application of methyl jasmonate in promoting the opening of cut peony flowers.

10. Application of methyl jasmonate in the preparation of formulations for promoting the opening of cut peony flowers.

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