Paecilomyces hepiali mixed powder and preparation method thereof

Abstract

The application discloses a paecilomyces hepiali and cordyceps militaris mixed powder and a preparation method thereof, and belongs to the technical field of functional food raw materials. The mixed powder takes paecilomyces hepiali mycelium powder and cordyceps militaris mycelium powder as core raw materials, and is compounded with a probiotic functional matrix and a natural antioxidant synergistic factor. Through an integrated process of liquid-phase uniform mixing and low-temperature co-drying, a uniform composite powder containing all components in a single particle is prepared. The application effectively solves the technical problems of traditional dry powder physical mixing, such as easy delamination, large loss of active ingredients, poor storage stability and low bioavailability. The product has high mixing uniformity, sufficient active ingredient reservation and a long shelf life, and can be widely applied to multiple fields, such as health foods, sports nutrition foods, special medical use formula foods and the like.

Description

Technical Field

[0001] This invention belongs to the field of functional food raw material technology, specifically relating to a mixed powder of Cordyceps militaris and Cordyceps sinensis from the bat moth and its preparation method. Background Technology

[0002] Cordyceps sinensis is a traditional and precious tonic in my country, recognized for its physiological activities in immune regulation, relieving physical fatigue, and organ maintenance. It has long enjoyed widespread application in both medicinal and edible fields. However, natural Cordyceps sinensis is limited by its growing environment, resulting in scarce resources, high market prices, and the risk of heavy metal accumulation during wild harvesting, making it difficult to meet the demands of large-scale industrial applications. Against this backdrop, Cordyceps mycelium powder prepared using artificial liquid deep fermentation technology has become a core alternative to natural Cordyceps sinensis. Among these, *Paecilomyces hepiali* (Cs-4) is a fungal strain approved by national authorities for use in health foods, and its fermented mycelium powder has been widely used in various health foods and pharmaceutical preparations.

[0003] To further broaden the efficacy coverage of Cordyceps militaris powder and enhance its comprehensive tonic effects, the industry has gradually seen the emergence of compound Cordyceps militaris powder products prepared by combining *Paecilomyces hepiali* mycelium powder with *Cordyceps sinensis* mycelium powder. The core idea is to combine the respective efficacy characteristics of the two mycelium powders to achieve complementary effects. However, currently, these mixed powder products generally adopt the traditional process route of "separate preparation - dry powder blending," which is essentially still a simple physical mixing of two independent mycelium powders, belonging to a typical two-phase dispersion system at the microscopic level. Due to the natural differences in the bulk density, particle size distribution, and powder flowability of the two mycelium powders, stratification and segregation are highly likely to occur during mixing, processing, transportation, and long-term storage. This results in significant fluctuations in the content of active ingredients in different parts of the final product, large fluctuations in product uniformity under conventional mixing processes, and significant differences in the content of active ingredients between batches and at different sites within the same batch, severely restricting the product's quality stability and efficacy consistency.

[0004] At the manufacturing process level, the existing production methods for Cordyceps militaris mixed powders are generally quite extensive, with key processes such as drying, pulverizing, and mixing generally lacking targeted protection measures for active ingredients. High-temperature drying environments, prolonged mechanical shearing, and open production environments can lead to varying degrees of thermal and oxidative degradation of core active ingredients such as adenosine and Cordyceps polysaccharides. Under most processes, core heat-sensitive active ingredients show significant degradation, directly affecting the actual efficacy of the product. At the same time, the formulation design of existing mixed powders generally remains at the level of simple algebraic superposition of the effects of two fungal powders, lacking the design and scientific ratio of synergistic components based on the pathway of action. Furthermore, the active ingredients are mostly encapsulated inside the fungal cell wall, resulting in limited dissolution and release efficiency in the human body and low bioavailability, making it difficult to fully realize the application value of the compound fungal powder.

[0005] Regarding storage stability, existing Cordyceps militaris mixed powders lack a stable structural design. The exposed powder particles exhibit strong hygroscopicity and oxidation sensitivity, making them prone to moisture absorption and clumping, as well as oxidation and deterioration of active ingredients under conventional storage conditions. During long-term storage at room temperature, the core active ingredients show significant degradation. Product quality is highly dependent on the barrier properties of packaging materials and storage conditions, which not only increases packaging and transportation costs but also limits the product's application in more scenarios.

[0006] In summary, developing a mixed powder of Cordyceps militaris with high mixing uniformity, good retention of active ingredients, strong storage stability, and synergistic effects, as well as its preparation method, is a technical problem that urgently needs to be solved in the field of deep processing of Cordyceps militaris powder. Summary of the Invention

[0007] The purpose of this invention is to address the shortcomings of existing technologies by providing a mixed powder of Cordyceps militaris and its preparation method. This invention systematically innovates from three dimensions: formulation system, microstructure, and preparation process, upgrading the traditional "two-phase physical mixed powder" to a "uniform structured composite powder," completely solving the defects of existing products such as easy stratification, low activity, poor stability, and limited efficacy.

[0008] To achieve the above objectives, the present invention adopts the following technical solution:

[0009] In a first aspect, the present invention provides a mixed powder of *Cordyceps militaris* and *Hepiali* fungi, comprising the following components by weight:

[0010] 30-80 parts of *Paecilomyces hepiali* mycelium powder.

[0011] 15-60 parts of Cordyceps sinensis mycelium powder

[0012] 5-15 parts of prebiotic functional matrix,

[0013] 0.1-2 parts of natural antioxidant synergist;

[0014] The mixed powder is a homogeneous composite powder containing all components within a single particle, which is obtained by homogenizing in the liquid phase and then drying and molding.

[0015] Preferably, the preferred components of the mixed powder, by weight, are:

[0016] 45-70 parts of *Paecilomyces hepiali* mycelium powder

[0017] 25-45 parts of Cordyceps sinensis mycelium powder

[0018] 8-12 parts of prebiotic functional matrix,

[0019] 0.3 to 1 part of natural antioxidant synergist.

[0020] Furthermore, the prebiotic functional matrix is ​​one or more combinations of fructooligosaccharides, yeast β-glucan, resistant dextrin, and inulin.

[0021] The prebiotic functional matrix has three functions: First, it promotes the absorption and metabolism of Cordyceps active ingredients by regulating the intestinal flora barrier, thus achieving dual-pathway synergistic effects; second, it modifies the powder, adjusts the loose density and particle size distribution of the mixed system, reduces the difference in physical properties between the two bacterial powders, and helps improve uniformity; third, it stabilizes and protects, acting as a dispersion matrix to encapsulate active ingredient particles and reduce their contact with oxygen and moisture.

[0022] Furthermore, the natural antioxidant synergist is one or more of rosemary extract, tea polyphenols, vitamin E, and selenomethionine. On the one hand, the natural antioxidant synergist scavenges free radicals from within the powder, inhibits the oxidative degradation of adenosine and polysaccharides, and improves the product's storage stability; on the other hand, it works synergistically with the active ingredients of cordyceps to enhance immune regulation and improve product efficacy.

[0023] Secondly, the present invention provides a method for preparing the above-mentioned mixed powder, specifically comprising the following steps:

[0024] S1. Mycelial pretreatment: *Paecilomyces hepiali* and *Cordyceps sinensis* were cultured in liquid submerged fermentation. After the fermentation reached the end point, wet mycelia were isolated and enriched, washed to remove residual culture medium, and then used for later use.

[0025] S2. Homogenization Blending: According to the target dry weight ratio, the two wet mycelia, prebiotic functional matrix, and natural antioxidant synergist are added to sterile water and fully dispersed to prepare a homogeneous and stable mycelial slurry.

[0026] S3. Low-temperature co-drying: The homogenized bacterial slurry is sent to a low-temperature vacuum belt dryer for drying. After removing the moisture, a composite dry powder is obtained.

[0027] S4. Protective post-treatment: The composite dry powder is subjected to closed-loop crushing and sieving, with inert gas protection throughout the process, to finally obtain a uniform composite powder with the target particle size, which is then sealed and packaged with nitrogen.

[0028] Furthermore, the specific steps of separation and enrichment in step S1 are as follows: enrich the wet mycelium by a combination of centrifugation and plate and frame filtration, wash with purified water 2-3 times to fully remove residual components of the culture medium and avoid impurities from affecting the uniformity of subsequent mixing and the purity of the product.

[0029] Furthermore, the specific parameters for dispersing the mycelium slurry in step S2 are as follows: the solid content of the system is controlled to be 10%~30%, and the dispersion is carried out by high-speed shearing with a shearing speed of 10000~15000 rpm and a dispersion time of 5~15 min, so that the mycelium and functional additives are fully broken and dispersed, and all components are uniformly distributed in the liquid phase, providing a basis for subsequent co-drying to form uniform composite particles.

[0030] Furthermore, the specific parameters of the drying process described in step S3 are as follows: the vacuum degree is controlled to be ≥0.09MPa during the drying process, and a segmented temperature-controlled drying mode is adopted: the first stage drying temperature is 45~55℃, which quickly removes most of the free water in the material; the second stage drying temperature is 35~45℃, which slowly removes the bound water and reduces the thermal degradation of active ingredients; the equipment belt speed is controlled to be 0.1~0.5m / min, and the drying endpoint is based on the material water activity ≤0.60, so as to maximize the retention of active ingredients while ensuring drying efficiency.

[0031] Further, the specific steps of the post-processing described in step S4 are as follows: the composite dry powder is fed into a low-temperature ultrafine pulverizer through a closed conveying system and pulverized under nitrogen protection. The temperature of the pulverizing chamber is controlled at 10~20℃, and the speed of the classifying wheel is 8000~15000rpm. After pulverization, it is classified by multi-stage vibrating sieve to obtain finished powder with a D90 of 10~50μm. The oxygen content of the entire post-processing system is ≤5%. The finished product is finally packaged in nitrogen-filled sealed packaging to isolate external oxygen and water vapor and ensure the long-term storage stability of the product.

[0032] Compared with the prior art, the present invention has the following beneficial technical effects:

[0033] 1. Outstanding Synergistic Effect of the Formula. This invention breaks through the limitations of traditional simple physical compounding of binary mycelial powders, constructing a synergistic formula system composed of core Cordyceps mycelial powder, prebiotic functional matrix, and natural antioxidant synergistic factors. Among them, prebiotics can regulate the intestinal microecological barrier, promote the absorption and metabolism of Cordyceps active ingredients in the body, and achieve synergistic effects through multiple pathways; natural antioxidant synergistic factors can not only work synergistically with Cordyceps active ingredients to exert physiological effects and enhance product activity, but also form antioxidant protection within the powder, thus improving the overall bioavailability and practical application value of the product.

[0034] 2. Excellent powder uniformity. This invention employs a preparation path of liquid-phase homogenization followed by integrated drying, which ensures that the active ingredients and functional additives of the two mycelia are uniformly dispersed within the same particle, forming a homogeneous composite powder structure. This eliminates the stratification and segregation problems caused by density and particle size differences in traditional dry powder mixing from a microscopic perspective, significantly improving the mixing uniformity of the product and ensuring the consistency of quality and efficacy between different parts and batches of the product.

[0035] 3. Excellent storage stability. This invention constructs a multi-layered stable protection system from the inside out by in-situ protection of antioxidant factors inside the powder, dispersion and encapsulation of prebiotic matrix, combined with inert gas protection throughout the preparation process and nitrogen-filled sealed packaging of the finished product. This effectively inhibits the oxidative degradation of active ingredients, reduces the risk of powder absorbing moisture and clumping, significantly extends the shelf life of the product, and reduces dependence on the barrier properties of packaging materials and storage environment conditions. Detailed Implementation

[0036] The present invention will be further described in detail below with reference to specific embodiments, but the scope of protection of the present invention is not limited to the content described.

[0037] Example 1

[0038] The mixed powder of *Paecilomyces hepiali* and *Cordyceps sinensis* in this embodiment comprises, by weight, 60 parts of *Paecilomyces hepiali* mycelium powder, 30 parts of *Cordyceps sinensis* mycelium powder, 8 parts of fructooligosaccharides, 0.5 parts of rosemary extract, and 0.3 parts of vitamin E.

[0039] The preparation method employs an integrated co-drying process, with the following steps:

[0040] S1. Mycelial pretreatment: *Paecilomyces hepiali* and *Cordyceps sinensis* were cultured in liquid submerged fermentation at a temperature of 26°C, an inoculum size of 6%, an aeration rate of 1.0 vvm, and a stirring speed of 180 rpm. The fermentation was terminated when the mycelial dry weight reached its peak. The wet mycelium was enriched by a combination of centrifugation and plate and frame filtration. It was washed twice with purified water to remove residual components of the culture medium before use.

[0041] S2. Homogenization and blending: According to the target dry weight ratio, the two wet mycelia, prebiotic functional matrix, and natural antioxidant synergist are added to sterile water. The solid content of the system is controlled at 20%. The mycelia and functional additives are dispersed by high-speed shearing at a speed of 12,000 rpm for 10 minutes to fully break down and disperse them, thus producing a homogeneous and stable mycelial slurry.

[0042] S3. Low-temperature co-drying: The homogenized bacterial slurry is fed into a low-temperature vacuum belt dryer for drying. The vacuum degree is controlled at 0.095MPa. A segmented temperature-controlled drying mode is adopted: the first stage drying temperature is 50℃, and the second stage drying temperature is 40℃. The belt speed of the equipment is controlled at 0.2m / min. The material is dried until the water activity is ≤0.60. After removing the moisture, a composite dry paste powder is obtained.

[0043] S4. Protective post-processing: The composite dry powder is fed into a low-temperature ultrafine pulverizer through a closed conveying system and pulverized under nitrogen protection. The temperature of the pulverizing chamber is controlled at 15℃ and the speed of the classifying wheel is 12000rpm. After pulverization, it is classified by multi-stage vibrating sieve to obtain a finished powder with a D90 of 25μm. The oxygen content of the entire post-processing system is ≤3%, and the finished product is finally packaged in nitrogen-filled sealed packaging.

[0044] Example 2

[0045] The mixed powder of *Paecilomyces hepiali* and *Cordyceps sinensis* in this embodiment comprises, by weight, 30 parts of *Paecilomyces hepiali* mycelium powder, 60 parts of *Cordyceps sinensis* mycelium powder, 5 parts of yeast β-glucan, and 0.1 parts of tea polyphenols.

[0046] The preparation method employs an integrated co-drying process, with the following steps:

[0047] S1. Mycelial pretreatment: *Paecilomyces hepiali* and *Cordyceps sinensis* were cultured in liquid submerged fermentation at a temperature of 25°C, an inoculum size of 5%, an aeration rate of 0.9 vvm, and a stirring speed of 170 rpm. The fermentation was terminated when the mycelial dry weight reached its peak. The wet mycelium was enriched by a combination of centrifugation and plate and frame filtration. It was washed three times with purified water to remove residual components of the culture medium before use.

[0048] S2. Homogenization and blending: According to the target dry weight ratio, the two wet mycelia, prebiotic functional matrix, and natural antioxidant synergist are added to sterile water. The solid content of the system is controlled at 10%. The mycelia are dispersed by high-speed shearing at a speed of 10,000 rpm for 15 minutes to fully break down and disperse the mycelia and functional additives, thus producing a homogeneous and stable mycelial slurry.

[0049] S3. Low-temperature co-drying: The homogenized bacterial slurry is fed into a low-temperature vacuum belt dryer for drying. The vacuum degree is controlled at 0.092MPa. A segmented temperature-controlled drying mode is adopted: the first stage drying temperature is 45℃, and the second stage drying temperature is 35℃. The belt speed of the equipment is controlled at 0.1m / min. The material is dried until the water activity is ≤0.60. After removing the moisture, a composite dry powder is obtained.

[0050] S4. Protective post-processing: The composite dry powder is fed into a low-temperature ultrafine pulverizer through a closed conveying system and pulverized under nitrogen protection. The temperature of the pulverizing chamber is controlled at 10℃ and the speed of the classifying wheel is 8000rpm. After pulverization, it is classified by multi-stage vibrating sieve to obtain finished powder with a D90 of 10μm. The oxygen content of the entire post-processing system is ≤5%, and the finished product is finally packaged in nitrogen-filled sealed packaging.

[0051] Example 3

[0052] The mixed powder of *Paecilomyces hepiali* and *Cordyceps sinensis* in this embodiment comprises, by weight, 80 parts of *Paecilomyces hepiali* mycelium powder, 15 parts of *Cordyceps sinensis* mycelium powder, 15 parts of resistant dextrin, and 2 parts of selenomethionine.

[0053] The preparation method employs an integrated co-drying process, with the following steps:

[0054] S1. Mycelial pretreatment: *Paecilomyces hepiali* and *Cordyceps sinensis* were cultured in liquid submerged fermentation at a temperature of 27°C, an inoculum size of 8%, an aeration rate of 1.1 vvm, and a stirring speed of 190 rpm. The fermentation was terminated when the mycelial dry weight reached its peak. The wet mycelium was enriched by a combination of centrifugation and plate and frame filtration. It was washed twice with purified water to remove residual components of the culture medium before use.

[0055] S2. Homogenization and blending: According to the target dry weight ratio, the two wet mycelia, prebiotic functional matrix, and natural antioxidant synergist are added to sterile water. The solid content of the system is controlled at 30%. The mycelia are dispersed by high-speed shearing at a speed of 15,000 rpm for 5 minutes to fully break down and disperse the mycelia and functional additives, thus producing a homogeneous and stable mycelial slurry.

[0056] S3. Low-temperature co-drying: The homogenized bacterial slurry is fed into a low-temperature vacuum belt dryer for drying. The vacuum degree is controlled at 0.098MPa. A segmented temperature-controlled drying mode is adopted: the first stage drying temperature is 55℃, and the second stage drying temperature is 45℃. The belt speed of the equipment is controlled at 0.5m / min. The material is dried until the water activity is ≤0.60. After removing the moisture, a composite dry powder is obtained.

[0057] S4. Protective post-processing: The composite dry powder is fed into a low-temperature ultrafine pulverizer through a closed conveying system and pulverized under nitrogen protection. The temperature of the pulverizing chamber is controlled at 20℃ and the speed of the classifying wheel is 15000rpm. After pulverization, it is classified by multi-stage vibrating sieve to obtain a finished powder with a D90 of 50μm. The oxygen content of the entire post-processing system is ≤4%, and the finished product is finally packaged in nitrogen-filled sealed packaging.

[0058] Example 4

[0059] The mixed powder of *Paecilomyces hepiali* and *Cordyceps sinensis* in this embodiment comprises, by weight, 45 parts of *Paecilomyces hepiali* mycelium powder, 45 parts of *Cordyceps sinensis* mycelium powder, 10 parts of inulin, and 1 part of rosemary extract.

[0060] The preparation method employs an integrated co-drying process, with the following steps:

[0061] S1. Mycelial pretreatment: *Paecilomyces hepiali* and *Cordyceps sinensis* were cultured in liquid submerged fermentation at a temperature of 26°C, an inoculum size of 7%, an aeration rate of 1.0 vvm, and a stirring speed of 180 rpm. The fermentation was terminated when the mycelial dry weight reached its peak. The wet mycelium was enriched by a combination of centrifugation and plate and frame filtration. It was washed twice with purified water to remove residual components of the culture medium before use.

[0062] S2. Homogenization and blending: According to the target dry weight ratio, the two wet mycelia, prebiotic functional matrix, and natural antioxidant synergist are added to sterile water. The solid content of the system is controlled at 20%. The mycelia and functional additives are dispersed by high-speed shearing at a speed of 12,000 rpm for 10 minutes to fully break down and disperse them, thus producing a homogeneous and stable mycelial slurry.

[0063] S3. Low-temperature co-drying: The homogenized bacterial slurry is fed into a low-temperature vacuum belt dryer for drying. The vacuum degree is controlled at 0.095MPa. A segmented temperature-controlled drying mode is adopted: the first stage drying temperature is 50℃, and the second stage drying temperature is 40℃. The equipment belt speed is controlled at 0.3m / min. The material is dried until the water activity is ≤0.60. After removing the moisture, a composite dry paste powder is obtained.

[0064] S4. Protective post-processing: The composite dry powder is fed into a low-temperature ultrafine pulverizer through a closed conveying system and pulverized under nitrogen protection. The temperature of the pulverizing chamber is controlled at 15℃ and the speed of the classifying wheel is 10,000 rpm. After pulverization, it is classified by multi-stage vibrating sieve to obtain a finished powder with a D90 of 30μm. The oxygen content of the entire post-processing system is ≤4%, and the finished product is finally sealed in nitrogen-filled packaging.

[0065] Comparative Example 1

[0066] The components of this comparative example are completely identical to those of Example 1, and it was prepared using a conventional dry powder physical mixing process in the art, with the following steps:

[0067] 1. *Paecilomyces hepiali* and *Cordyceps sinensis* were fermented separately in a liquid submerged fermentation chamber at a temperature of 26°C, an inoculum size of 6%, an aeration rate of 1.0 vvm, and a stirring speed of 180 rpm. The fermentation was terminated when the mycelial dry weight reached its peak. Dry mycelial powder was prepared separately by hot air drying, and then pulverized and sieved at room temperature to obtain two single mycelial powder raw materials.

[0068] 2. Weigh out the two types of bacterial powder, fructooligosaccharide, rosemary extract, and vitamin E according to the formula, and put them into a three-dimensional motion mixer. Mix for 30 minutes at room temperature and pressure.

[0069] 3. After mixing, discharge the material and seal it in a standard package to obtain the finished mixed powder.

[0070] Comparative Example 2

[0071] This comparative example uses only two types of Cordyceps mycelium powder as raw materials, without adding prebiotic functional matrix and natural antioxidant synergistic factors. The preparation process is completely consistent with the co-drying integrated process of Example 1. The formula by weight is: 60 parts of Paecilomyces hepiali mycelium powder and 40 parts of Cordyceps sinensis mycelium powder.

[0072] Comparative Example 3

[0073] The components of this comparative example are completely identical to those of Example 1, except that the drying process is replaced with conventional hot air drying and the pulverizing process is replaced with room temperature open-type pulverizing. The remaining steps are the same as in Example 1, specifically:

[0074] S3. The drying step uses a hot air drying oven at 60℃ and normal pressure until the water activity is ≤0.60.

[0075] S4. The pulverizing step uses a common universal pulverizer, and the pulverizing and sieving are carried out in an open environment at room temperature without the use of inert gas protection. The remaining steps are the same as in Example 1.

[0076] Comparative Example 4

[0077] This comparative example uses only Paecilomyces pilosa mycelium powder as a single raw material. The preparation process is completely consistent with the co-drying integrated process of Example 1. The formula by weight is: 100 parts of Paecilomyces pilosa mycelium powder.

[0078] Performance Testing and Result Analysis

[0079] The samples prepared in the above embodiments and comparative examples were subjected to performance testing. The testing items included mixing uniformity, active ingredient retention rate, accelerated storage stability, and in vitro dissolution rate. The testing methods are as follows:

[0080] 1. Mixing uniformity: Referring to the powder mixing uniformity test method, samples were taken from five different locations (top, middle, bottom, left, and right) of the finished powder. The adenosine content was determined by high performance liquid chromatography, and the relative standard deviation (RSD) was calculated. The smaller the RSD, the better the mixing uniformity.

[0081] 2. Retention rate of active ingredients: The initial contents of adenosine and cordyceps polysaccharide in the mycelium at the end of fermentation and the contents of the corresponding components in the finished product were measured respectively, and the retention rate was calculated. The higher the retention rate, the better the protection effect of the process on the active ingredients.

[0082] 3. Accelerate storage stability: Place the sample in a constant temperature and humidity chamber at 40℃ and 75% relative humidity for 6 months to accelerate storage. Measure the adenosine content after storage and calculate the retention rate relative to the initial product. A higher retention rate indicates better storage stability.

[0083] 4. In vitro dissolution: The dissolution rate was determined using the paddle method with artificial gastric fluid as the dissolution medium. The cumulative dissolution rate of adenosine was measured at 37°C and 50 rpm for 30 min. The higher the dissolution rate, the better the bioavailability.

[0084] The test results are shown in the table below:

[0085] Table 1 Performance test results of each embodiment and comparative example

[0086] Testing items Example 1 Example 2 Example 3 Example 4 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Mixing uniformity RSD (%) 1.2 1.1 1.4 1.3 6.3 1.3 1.5 / Adenosine retention rate (%) 96.2 95.8 95.5 96.0 76.4 94.7 72.1 95.9 Cordyceps polysaccharide retention rate (%) 95.7 95.3 95.1 95.5 78.3 94.9 70.5 95.6 Accelerated adenosine retention rate at 6 months (%) 91.3 90.5 92.1 91.7 68.2 82.7 65.3 80.4 In vitro dissolution rate of adenosine (%) at 30 min 82.5 80.3 81.7 83.1 58.6 76.8 57.2 75.9

[0087] Results analysis:

[0088] 1. As can be seen from the comparison between the examples and Comparative Example 1, the mixed powder prepared by the co-drying integrated process of the present invention has a much better mixing uniformity than the traditional dry powder physical mixing process. It eliminates the problem of stratification and segregation caused by the difference in density and particle size of the two bacterial powders at the microstructure level, and significantly improves the uniformity of product quality. At the same time, the retention rate of active ingredients and accelerated stability are also significantly better, proving that the low-temperature protective process and low oxygen control throughout the process of the present invention can effectively reduce the thermal degradation and oxidation loss of active ingredients.

[0089] 2. As can be seen from the comparison between the examples and Comparative Example 2, under the same drying process conditions, the formulation with added prebiotic functional matrix and natural antioxidant synergist significantly improves both storage stability and in vitro dissolution. This proves that the functional additives can not only encapsulate active ingredients and improve storage stability through matrix dispersion, but also improve the dissolution performance of powder and promote the in vivo release of active ingredients, thus verifying the synergistic effect of the ternary formulation system of the present invention.

[0090] 3. As can be seen from the comparison between the examples and Comparative Example 3, the whole process of low-temperature vacuum belt drying + inert gas protected pulverization of the present invention has a much higher retention rate of active ingredients than conventional hot air drying + open pulverization. This proves that the low-temperature and low-oxygen protective preparation strategy of the present invention can effectively avoid the destruction of heat-sensitive active ingredients such as cordyceps polysaccharides and adenosine, and retain the natural active substances in the mycelium to the maximum extent.

[0091] 4. As can be seen from the comparison between the examples and Comparative Example 4, the compound fungal powder formula of the present invention, combined with the synergistic effect of functional additives, is superior to single fungal powder in terms of active ingredient dissolution and storage stability. It can achieve the complementarity and synergy of the effects of two Cordyceps fungi, and expand the efficacy coverage and application scenarios of the product.

[0092] 5. Examples 1-4 respectively cover the endpoint and intermediate values ​​of the component ratio of the present invention. All performance indicators are stable and excellent, proving that the formulation range and process parameters of the present invention have good industrial feasibility and batch stability, and can stably prepare high-quality Cordyceps militaris mixed powder products.

Claims

1. A mixed powder of *Cordyceps militaris* and *Hepialus chinensis*, characterized in that, The mixture comprises the following components by weight: 30-80 parts of *Paecilomyces hepiali* mycelium powder, 15-60 parts of *Cordyceps sinensis* mycelium powder, 5-15 parts of prebiotic functional matrix, and 0.1-2 parts of natural antioxidant synergist; the mixed powder is a homogeneous composite powder containing all components in a single particle, which is prepared by liquid-phase homogenization followed by co-drying and molding.

2. The mixed powder of *Cordyceps militaris* and *Hepiali* according to claim 1, characterized in that, By weight, it includes the following components: 45-70 parts of *Paecilomyces hepiali* mycelium powder, 25-45 parts of *Cordyceps sinensis* mycelium powder, 8-12 parts of prebiotic functional matrix, and 0.3-1 part of natural antioxidant synergist.

3. The mixed powder of *Cordyceps militaris* and *Hepiali* according to claim 1, characterized in that, The prebiotic functional matrix is ​​one or more of the following: fructooligosaccharides, yeast β-glucan, resistant dextrin, and inulin.

4. The mixed powder of *Cordyceps militaris* and *Hepiali* according to claim 1, characterized in that, The natural antioxidant synergist is one or more of the following: rosemary extract, tea polyphenols, vitamin E, and selenomethionine.

5. A method for preparing a mixed powder of *Cordyceps militaris* and *Hepiali* according to any one of claims 1 to 4, characterized in that, The integrated co-drying process includes the following steps: S1. Mycelial pretreatment: *Paecilomyces hepiali* and *Cordyceps sinensis* were cultured in liquid submerged fermentation. After the fermentation reached the end point, wet mycelia were isolated and enriched. After washing to remove residual culture medium, they were used for later use. S2. Homogenization and blending: According to the target dry weight ratio, the two wet mycelia, prebiotic functional matrix and natural antioxidant synergist are added to sterile water and fully dispersed to make a homogeneous and stable mycelial slurry. S3. Low-temperature co-drying: The homogenized bacterial slurry is sent to a low-temperature vacuum belt dryer for drying. After removing the moisture, a composite dry powder is obtained. S4. Protective post-treatment: The composite dry powder is subjected to closed-loop crushing and sieving, with inert gas protection throughout the process, to finally obtain a uniform composite powder with the target particle size, which is then sealed and packaged with nitrogen.

6. The preparation method according to claim 5, characterized in that, In step S1, the separation and enrichment adopts a combination of centrifugal separation and plate and frame filtration, and the washing is performed with purified water 2 to 3 times.

7. The preparation method according to claim 5, characterized in that, In step S2, the solid content of the system is controlled to be 10%~30%, and dispersion is carried out by high-speed shearing with a shearing speed of 10000~15000 rpm and a dispersion time of 5~15 min.

8. The preparation method according to claim 5, characterized in that, In step S3, the vacuum degree is controlled to be ≥ during the drying process. 0.09MPa, using segmented temperature control mode: the first stage drying temperature is 45~55℃, the second stage drying temperature is 35~45℃; the equipment belt speed is 0.1~0.5m / min, and the moisture activity of the material is controlled to be ≤0.60 at the drying endpoint.

9. The preparation method according to claim 5, characterized in that, In step S4, a low-temperature ultrafine pulverizer is used for pulverization. Nitrogen is used as the protective gas during the pulverization process. The temperature of the pulverizing chamber is controlled at 10~20℃, and the speed of the classifying wheel is 8000~15000rpm. After pulverization, the finished powder with a D90 of 10~50μm is obtained by multi-stage vibrating sieve.

10. The preparation method according to claim 5, characterized in that, In step S4, the oxygen content of the entire post-treatment system is ≤5%.