Silicon-containing water for improving expression lines and method of preparing the same

Abstract

This invention relates to the field of cosmetic technology, specifically to a silicon-based water for improving expression lines and its preparation method; comprising, by weight, the following components: 40-70 parts of plant-derived silicon-based water concentrate, wherein the silicon concentration in the plant-derived silicon-based water concentrate is 8000-12000 ppm, wherein the silicon exists in the form of monomolecular orthosilicic acid or its oligomers, and the hydrated particle size is ≤0.4 nm; 0.005-0.1 parts of acetyl hexapeptide-8; 0.01-0.5 parts of silanized hyaluronic acid, wherein the relative molecular mass of the silanized hyaluronic acid is 50-100 kDa, and the silicon content is ≥0.5%; 8-12 parts of moisturizer; 0.5-2 parts of panthenol; 0.1-0.5 parts of asiaticoside; 0.01-0.1 parts of acetylated sodium hyaluronate; 0.1-0.5 parts of solubilizer; 0.35-1 part of preservative; and the balance being deionized water. This invention uses silicon-based water as the main delivery medium, and utilizes the superpermeability of water-soluble silicon particles with a particle size ≤0.4nm to enhance the transdermal delivery efficiency of acetyl hexapeptide-8. Franz diffusion cell experiments confirmed that the cumulative permeation increased by 3.1 to 3.7 times in 12 hours, thus solving the technical problem of low transdermal absorption rate of peptide active ingredients.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, specifically to a silicon-based water for improving expression lines and its preparation method. Background Technology

[0002] Expression lines are dynamic wrinkles formed by the repeated contraction of facial muscles, resulting in localized skin folds. They mainly include forehead wrinkles, frown lines, crow's feet, and nasolabial folds. The formation mechanism of expression lines involves the release of acetylcholine at the neuromuscular junction, which triggers the contraction of facial muscles. Long-term repeated contractions cause structural damage to collagen and elastic fibers in the dermis, and the dynamic lines gradually solidify into static lines.

[0003] CN117695160A discloses a silicone water anti-wrinkle and firming cream, which uses 5-20 parts of silicone water in combination with snake venom peptides and cone snail venom to achieve anti-wrinkle and firming effects. However, it is a cream formulation, and silicone water is only added as one component in the formula, which does not give full play to the penetration and delivery advantages of silicone water. Although snake venom peptides and cone snail venom have muscle-relaxing effects, they are not targeted at the dynamic characteristics of expression lines. Moreover, the oil components in the cream formulation may hinder the penetration of active ingredients to the neuromuscular junction.

[0004] CN111407719A discloses a skin care product containing water-soluble silicon, which uses 3-5 parts of water-soluble silicon in combination with plant extracts such as Kappa algae extract and Wakame seaweed extract to achieve whitening, moisturizing and anti-aging. However, its technical solution focuses on whitening and moisturizing, does not involve targeted improvement of expression lines, lacks muscle relaxation mechanism, and cannot block the dynamic formation process of expression lines from the source.

[0005] CN114569490B discloses a composition with moisturizing, skin barrier repair, anti-wrinkle, and firming effects, using low molecular weight hyaluronic acid silanol in combination with xylitol moisturizing factor to achieve moisturizing and anti-wrinkle effects. However, this solution does not address the targeted improvement of expression lines, does not contain muscle-relaxing components, and cannot intervene in the dynamic causes of expression lines. Existing technologies also include solutions that combine acetyl hexapeptide-8 with silanol compounds for anti-wrinkle purposes, but these solutions all use conventional deionized water as a solvent system, failing to recognize the enhancing effect of silanized water as a delivery medium on the transdermal absorption of peptide active ingredients. Furthermore, the silanol compounds used are fundamentally different from the silanized hyaluronic acid used in this invention in terms of chemical structure and mechanism of action.

[0006] Therefore, the existing technology has the following shortcomings: there is no product that uses silicon water as the main delivery medium to target and improve expression lines; the super-permeability of silicon water has not been utilized to enhance the transdermal delivery of peptide active ingredients; and there is a lack of an expression line improvement system that simultaneously enhances penetration and delivery, relaxes muscles, and reconstructs dermal structures. Summary of the Invention

[0007] The primary objective of this invention is to provide a silicon-based water for improving expression lines and a method for preparing the same.

[0008] A further objective of this invention is to provide a silicon-based water for improving expression lines, comprising the following components by weight: 40-70 parts of plant-derived silicon water concentrate, wherein the silicon concentration in the plant-derived silicon water concentrate is 8000-12000 ppm, wherein the silicon exists in the form of monomolecular orthosilicic acid or its oligomers, and the hydrated particle size is ≤0.4 nm; 0.005-0.1 parts of acetyl hexapeptide-8; 0.01-0.5 parts of silanized hyaluronic acid, wherein the relative molecular mass of the silanized hyaluronic acid is 50-100 kDa, and the silicon content is ≥0.5%; 8-12 parts of moisturizer; 0.5-2 parts of panthenol; 0.1-0.5 parts of asiaticoside; 0.01-0.1 parts of acetylated sodium hyaluronate; 0.1-0.5 parts of solubilizer; 0.35-1 part of preservative; and the balance being deionized water.

[0009] Preferably, the plant-derived silicon water concentrate is obtained by high-temperature alkaline extraction of barley husks or rice husks, with a silicon concentration of 9000-10000 ppm and a pH value of 7.0-8.5.

[0010] 3. The silicon water for improving expression lines as described in claim 1, characterized in that the purity of the acetyl hexapeptide-8 is ≥97%, and its addition amount is 0.01-0.05 parts by mass.

[0011] Preferably, the degree of substitution of silanol groups in the silanized hyaluronic acid is 0.5-2.0%, and its relative molecular mass is 60-80 kDa.

[0012] Preferably, the moisturizer is composed of glycerin and 1,3-propanediol, wherein the mass ratio of glycerin to 1,3-propanediol is 1:0.4 to 1:0.8.

[0013] Preferably, the solubilizer is PEG-40 hydrogenated castor oil, and the preservative is composed of phenoxyethanol and ethylhexylglycerin, with a mass ratio of phenoxyethanol to ethylhexylglycerin of 5:1.

[0014] A method for preparing the aforementioned silicon-based water for improving expression lines includes the following steps: Step 1: Mix the plant-derived silicon water concentrate with deionized water and stir until homogeneous to obtain silicon water base solution. Control the system temperature at 35-40℃. Step 2: Add humectant and panthenol to the silicon aqueous base solution, and stir at 200-300 r / min for 15-25 min until completely dissolved to obtain aqueous phase mixture I; Step 3: Cool the aqueous phase mixture I to 30-35℃, add silanized hyaluronic acid, asiaticoside and acetylated sodium hyaluronate, and stir at 150-250 r / min for 10-20 min until completely dissolved to obtain aqueous phase mixture II; Step 4: Cool the aqueous phase mixture II to 25-28℃, add acetyl hexapeptide-8, and stir at 100-200 r / min for 5-10 min until uniformly dispersed to obtain aqueous phase mixture III; Step 5: Add solubilizer and preservative to aqueous mixture III, stir at 100-200 r / min for 5-10 min, filter through a 0.22 μm microporous membrane, and fill into a container to obtain silicon water that improves expression lines.

[0015] Preferably, the silicon concentration in the silicon aqueous substrate solution in step one is 5000-9000 ppm, and the addition temperature of acetyl hexapeptide-8 in step four is controlled below 28°C.

[0016] Compared with the prior art, the beneficial effects of the present invention are: 1. This invention uses silicon-based water as the main delivery medium, and utilizes the superpermeability of water-soluble silicon particles with a particle size ≤0.4nm to enhance the transdermal delivery efficiency of acetyl hexapeptide-8. Franz diffusion cell experiments have confirmed that the cumulative permeation amount is increased by 3.1 to 3.7 times in 12 hours, thus solving the technical problem of low transdermal absorption rate of peptide active ingredients.

[0017] 2. This invention forms a three-pathway synergistic system of penetration delivery, muscle relaxation, and structural reconstruction, which reduces the area of ​​expression lines by 38.7%-42.3% in 28 days, significantly better than the use of individual components or existing technical solutions.

[0018] 3. The preparation method of this invention adopts a gradient cooling and stepwise addition strategy. The moisturizer and panthenol are dissolved at 35-40℃ to ensure dissolution efficiency. Silanized hyaluronic acid and centella asiatica glycoside are added at 30-35℃ to avoid high-temperature degradation. Acetyl hexapeptide-8 is added below 28℃ to protect peptide chain activity. All active components maintain a high activity state, and the product has excellent comprehensive performance. After the plant-derived silicon water concentrate is mixed and diluted with deionized water, the pH value drops to a near-neutral range of 6.5 to 8.0, which not only retains the permeation and delivery function of silicon water, but also provides a suitable pH environment for chemical stability for acetyl hexapeptide-8 and silanized hyaluronic acid.

[0019] 4. This invention is a water-based product with a refreshing texture, rapid absorption, no oil content, and does not clog pores. It is suitable for all skin types, and is especially suitable for oily and combination skin to improve expression lines. Detailed Implementation

[0020] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0021] The mechanism by which this invention improves expression lines is based on a three-pathway system: Penetration and delivery pathway: Plant-derived silicon water contains water-soluble silicon particles with a particle size ≤0.4nm, which is much smaller than the 50-100nm gap between skin cells, and has a super strong ability to penetrate the stratum corneum.

[0022] Water-soluble silicon particles enhance the delivery efficiency of acetyl hexapeptide-8 during transdermal transmission through the following mechanisms: Water-soluble silicon particles interact with the lipid bilayer of the stratum corneum, temporarily increasing the fluidity of the lipid bilayer and forming hydrophilic permeation channels, allowing acetyl hexapeptide-8, which is originally difficult to transdermally due to its large molecular weight and strong hydrophilicity, to penetrate into the dermis through these channels; At the same time, the hydroxyl groups on the surface of water-soluble silicon particles form hydrogen bonds with the polar amino acid residues of acetyl hexapeptide-8, acting as a carrier during transdermal transmission and carrying peptide molecules across the stratum corneum barrier.

[0023] Franz diffusion cell experiments confirmed that the 12-hour cumulative permeation of acetyl hexapeptide-8 using siliceous water as the medium was 3.1 to 3.7 times that using conventional deionized water as the medium.

[0024] It should be noted that the water-soluble silicon particle size ≤0.4nm refers to the size of hydrated silicon particles existing in aqueous solution as single molecules of orthosilicic acid Si(OH)4 or its oligomers, determined by small-angle X-ray scattering or calculated by orthosilicic acid molecular size theory. This size is much smaller than the 50 to 100nm threshold of skin cell intercellular space, which is the physical basis for superpermeability. The water-soluble silicon particles described in this invention are fundamentally different from ordinary silica sol: the silica particles in ordinary silica sol have a particle size of 10 to 20nm, which is much larger than the intercellular space of skin cells and cannot penetrate the stratum corneum, thus lacking the ability to enhance penetration; while the water-soluble silicon particles in plant-derived silicon water exist in the form of single molecules of orthosilicic acid, with a particle size of only about 0.4nm, and can achieve superpermeable delivery through the stratum corneum intercellular space. The particle size difference between the two is 25 to 50 times, and they cannot be substituted for each other.

[0025] Muscle relaxation pathway: Acetyl hexapeptide-8, delivered efficiently to the neuromuscular junction in the dermis via a silicon-aqueous medium, mimics the N-terminal functional domain of the synaptic protein SNAP-25. It competitively binds to VAMP and Syntaxin proteins in the SNARE complex, forming an unstable and ineffective assembly. This hinders the proper assembly of the SNARE complex, reduces the release of acetylcholine from the presynaptic membrane, and inhibits excessive contraction of facial muscles, thus reducing the dynamic formation of expression lines at its source. Unlike traditional botulinum toxin injections that cleave SNAP-25 protein, acetyl hexapeptide-8 exhibits reversible competitive binding, without damaging the protein structure or causing muscle paralysis, preserving natural facial expressions. It should be noted that the molecular structure of acetyl hexapeptide-8 contains methionine residues, which are prone to oxidative inactivation under high temperature and alkaline conditions. This invention employs a gradient cooling stepwise addition strategy, controlling the addition temperature of acetyl hexapeptide-8 below 28°C to effectively avoid thermally oxidative inactivation. Meanwhile, the pH value of the plant-derived silicon water concentrate is 7.0 to 8.5. After being diluted with deionized water, the pH value of the silicon water base solution drops to a near-neutral range of 6.5 to 8.0. Under these pH conditions, acetyl hexapeptide-8 has good chemical stability and can meet the stability requirements of cosmetics for a shelf life of 2 to 3 years.

[0026] Structural Reconstruction Pathway: Silanized hyaluronic acid is a hyaluronic acid derivative with silanol groups introduced into the hyaluronic acid molecule, combining the moisturizing function of hyaluronic acid with the structural reconstruction function of silanol. The silanol groups in silanized hyaluronic acid cross-link with the hydroxyl groups of type I collagen fibers in the dermis, increasing the cross-linking density between collagen fibers and improving the structural stability of the dermis. Simultaneously, the silanol groups activate the activity of prolyl hydroxylase in fibroblasts, promoting the synthesis of type I and type III collagen, increasing the thickness and density of the dermis. The hyaluronic acid backbone in silanized hyaluronic acid provides a highly moisturizing microenvironment, promoting fibroblast proliferation and migration.

[0027] Silanized hyaluronic acid and silicon in the silica-based water form a progressive structural repair chain of silicon, silanol, and hyaluronic acid: water-soluble silicon particles in the silica-based water replenish the silicon lost from the dermis, providing catalytic conditions for collagen synthesis; silanized hyaluronic acid releases silanol groups in the dermis, directly participating in collagen cross-linking; the hyaluronic acid framework maintains a hydrating environment and ensures cell activity. It should be noted that the silanol groups in silanized hyaluronic acid may undergo condensation reactions to form siloxane bonds under alkaline conditions. However, in the formulation system of this invention, the silica-based water base solution, after dilution, has a near-neutral pH range of 6.5 to 8.0, and the amount of silanized hyaluronic acid added is only 0.01 to 0.5 parts. Under low concentration and near-neutral conditions, the condensation reaction rate of the silanol groups is extremely low, which will not lead to cross-linking aggregation or abnormal increase in system viscosity, resulting in good storage stability of the formulation.

[0028] The improved penetration and delivery efficiency of the first pathway significantly enhances the muscle-relaxing effect of the second pathway. The reduction of facial muscle contraction in the second pathway consolidates the effect of the third pathway's structural reconstruction, preventing it from being damaged by repeated mechanical stress. The improved dermal microenvironment after the third pathway's structural reconstruction provides a more favorable diffusion path for the penetration and delivery of the first pathway.

[0029] Raw material source information: Plant-derived silicon water concentrate: silicon concentration 9300ppm, obtained by high-temperature alkaline extraction of barley hulls at 650℃, water-soluble silicon particle size ≤0.4nm (determined by small-angle X-ray scattering), pH value 7.8, source: Tibet Silicon Water Technology Development Co., Ltd. Plant-derived silicon water concentrate: silicon concentration 12000ppm, obtained by high-temperature alkaline extraction of rice husks at 700℃, water-soluble silicon particle size ≤0.4nm (determined by small-angle X-ray scattering), pH value 8.2, source: Tibet Silicon Water Technology Development Co., Ltd. Acetyl hexapeptide-8: purity ≥97%, CAS No. 616204-22-9, source: Lipotec; Silanized hyaluronic acid: relative molecular mass 60-80 kDa, silicon content 0.8%, silanol group substitution degree 1.2%, prepared by catalytic reaction of sodium hyaluronate and silane coupling agent, with silanol groups covalently grafted onto the hydroxyl groups of the hyaluronic acid molecular chain, sourced from Shandong Freda Bioengineering Co., Ltd. Glycerin: Purity ≥ 99.5%, sourced from Hunan Ercon Pharmaceutical Co., Ltd.; 1,3-Propanediol: Purity ≥99.5%, sourced from DuPont; Panthenol: Purity ≥98%, CAS No. 81-13-0, source: DSM; Centella asiatica extract: purity ≥95%, CAS No. 1405-44-9, source: Xi'an Lvtian Biotechnology Co., Ltd.; Acetylated sodium hyaluronate: relative molecular mass 30-50 kDa, degree of acetylation ≥60%, sourced from Bloomage Biotechnology Co., Ltd. PEG-40 hydrogenated castor oil: purity ≥99%, sourced from BASF; Phenoxyethanol: Purity ≥99%, sourced from Lanxess; Ethylhexylglycerin: purity ≥98%, sourced from Symrise Corporation; Sodium hyaluronate: relative molecular mass 60-80 kDa, source: Bloomage Biotechnology Co., Ltd. Snake venom peptide: purity ≥95%, sourced from Lipotec; Conodon toxin: purity ≥95%, sourced from Lipotec. Collagen: relative molecular mass 3-5 kDa, sourced from Rousselot; Silica sol: Particle size 10-20nm, SiO2 content 30%, pH value 9.5, source: Zhejiang Yuda Chemical Co., Ltd. Kappa longifolia extract: solid content ≥50%, sourced from Guangzhou Youke Biotechnology Co., Ltd.; Wakame seaweed extract: solid content ≥50%, sourced from Guangzhou Youke Biotechnology Co., Ltd.; Nicotinamide: Purity ≥99%, sourced from DSM. Lysine: Purity ≥98%, sourced from Ajinomoto Co., Ltd., Japan; Lecithin: purity ≥95%, sourced from Lipoid GmbH, Germany; Deionized water: conductivity ≤5μS / cm, homemade.

[0030] Example 1:

[0031] Raw material formula: 50.0 parts by weight of plant-derived silicon aqueous concentrate (silicon concentration 9300ppm), 39.60 parts by weight of deionized water (conductivity ≤5μS / cm), 5.00 parts by weight of glycerol (purity ≥99.5%), 3.00 parts by weight of 1,3-propanediol (purity ≥99.5%), 1.00 parts by weight of panthenol (purity ≥98%), 0.10 parts by weight of silanized hyaluronic acid (relative molecular mass 60-80kDa, silicon content 0.8%), and Centella asiatica. 0.30 parts by weight of oxalool (purity ≥95%), 0.05 parts by weight of acetylated sodium hyaluronate (relative molecular mass 30-50 kDa, degree of acetylation ≥60%), 0.05 parts by weight of acetylated hexapeptide-8 (purity ≥97%), 0.30 parts by weight of PEG-40 hydrogenated castor oil (purity ≥99%), 0.50 parts by weight of phenoxyethanol (purity ≥99%), and 0.10 parts by weight of ethylhexylglycerin (purity ≥98%), totaling 100.00 parts by weight. Of these, 8.00 parts by weight are humectants (glycerin and 1,3-propanediol), and 0.60 parts by weight are preservatives (phenoxyethanol and ethylhexylglycerin).

[0032] Preparation process: Step 1: Add 50.0 parts by weight of plant-derived silicon water concentrate and 39.60 parts by weight of deionized water to a mixing tank and stir at 300 r / min for 10 min to obtain silicon water base solution. The system temperature is controlled at 38℃. Step 2: Add 5.00 parts by weight of glycerol, 3.00 parts by weight of 1,3-propanediol and 1.00 parts by weight of panthenol to the silicon aqueous base solution, and stir at 250 r / min for 20 min until completely dissolved to obtain aqueous phase mixture I; Step 3: Cool the aqueous phase mixture I to 32°C, add 0.10 parts by mass of silanized hyaluronic acid, 0.30 parts by mass of asiaticoside and 0.05 parts by mass of acetylated sodium hyaluronate, and stir at 200 r / min for 15 min until completely dissolved to obtain aqueous phase mixture II; Step four: Cool aqueous phase mixture II to 27°C, add 0.05 parts by mass of acetyl hexapeptide-8, and stir at 150 r / min for 8 min until uniformly dispersed to obtain aqueous phase mixture III. Step 5: Add 0.30 parts by weight of PEG-40 hydrogenated castor oil, 0.50 parts by weight of phenoxyethanol and 0.10 parts by weight of ethylhexylglycerin to aqueous phase mixture III, stir at 150 r / min for 8 min, filter through a 0.22 μm microporous membrane, and fill into a container to obtain silicone water that improves expression lines.

[0033] In this embodiment, the silicon concentration of the silicon-based aqueous solution was approximately 5190 ppm, and the final product pH value was approximately 6.8. After 28 days, the area of ​​expression lines decreased by 38.7%, the skin elasticity R2 value improved by 31.2%, the muscle contraction amplitude decreased by 26.5%, the stratum corneum moisture content increased by 45.3%, and the 12-hour cumulative penetration of acetyl hexapeptide-8 was 3.87 μg / cm2.

[0034] Example 2:

[0035] Raw material formula: 65.0 parts by weight of plant-derived silicon aqueous concentrate (silicon concentration 12000ppm), 24.60 parts by weight of deionized water (conductivity ≤5μS / cm), 5.00 parts by weight of glycerol (purity ≥99.5%), 3.00 parts by weight of 1,3-propanediol (purity ≥99.5%), 1.00 parts by weight of panthenol (purity ≥98%), 0.10 parts by weight of silanized hyaluronic acid (relative molecular mass 60-80kDa, silicon content 0.8%), and... Asiaticoside (purity ≥95%) 0.30 parts by weight, sodium acetylated hyaluronic acid (relative molecular mass 30-50 kDa, degree of acetylation ≥60%) 0.05 parts by weight, acetylated hexapeptide-8 (purity ≥97%) 0.05 parts by weight, PEG-40 hydrogenated castor oil (purity ≥99%) 0.30 parts by weight, phenoxyethanol (purity ≥99%) 0.50 parts by weight, ethylhexylglycerin (purity ≥98%) 0.10 parts by weight, totaling 100.00 parts by weight.

[0036] Preparation process: Step 1: Add 65.0 parts by weight of plant-derived silicon water concentrate and 24.60 parts by weight of deionized water to a mixing tank and stir at 300 r / min for 10 min to obtain silicon water base solution. The system temperature is controlled at 36℃. Step 2: Add 5.00 parts by weight of glycerol, 3.00 parts by weight of 1,3-propanediol and 1.00 parts by weight of panthenol to the silicon aqueous base solution, and stir at 250 r / min for 20 min until completely dissolved to obtain aqueous phase mixture I; Step 3: Cool the aqueous phase mixture I to 33°C, add 0.10 parts by mass of silanized hyaluronic acid, 0.30 parts by mass of asiaticoside and 0.05 parts by mass of acetylated sodium hyaluronate, and stir at 200 r / min for 15 min until completely dissolved to obtain aqueous phase mixture II; Step 4: Cool the aqueous phase mixture II to 26°C, add 0.05 parts by mass of acetyl hexapeptide-8, and stir at 150 r / min for 8 min until uniformly dispersed to obtain aqueous phase mixture III; Step 5: Add 0.30 parts by weight of PEG-40 hydrogenated castor oil, 0.50 parts by weight of phenoxyethanol and 0.10 parts by weight of ethylhexylglycerin to aqueous phase mixture III, stir at 150 r / min for 8 min, filter through a 0.22 μm microporous membrane, and fill into a container to obtain silicone water that improves expression lines.

[0037] In this embodiment, the silicon concentration of the silicon-based aqueous solution was approximately 8705 ppm, and the final product pH value was approximately 7.2. After 28 days, the area of ​​expression lines decreased by 42.3%, the skin elasticity R2 value improved by 34.8%, the muscle contraction amplitude decreased by 29.1%, the stratum corneum moisture content increased by 48.7%, and the 12-hour cumulative penetration of acetyl hexapeptide-8 was 4.52 μg / cm2.

[0038] Example 3:

[0039] Raw material formula: 50.0 parts by weight of plant-derived silicon aqueous concentrate (silicon concentration 9300ppm), 39.45 parts by weight of deionized water (conductivity ≤5μS / cm), 5.00 parts by weight of glycerol (purity ≥99.5%), 3.00 parts by weight of 1,3-propanediol (purity ≥99.5%), 1.00 parts by weight of panthenol (purity ≥98%), 0.20 parts by weight of silanized hyaluronic acid (relative molecular mass 60-80kDa, silicon content 0.8%), and Centella asiatica. 0.30 parts by weight of oxalool (purity ≥95%), 0.05 parts by weight of acetylated sodium hyaluronate (relative molecular mass 30-50 kDa, degree of acetylation ≥60%), 0.10 parts by weight of acetylated hexapeptide-8 (purity ≥97%), 0.30 parts by weight of PEG-40 hydrogenated castor oil (purity ≥99%), 0.50 parts by weight of phenoxyethanol (purity ≥99%), and 0.10 parts by weight of ethylhexylglycerin (purity ≥98%), totaling 100.00 parts by weight.

[0040] Preparation process: Step 1: Add 50.0 parts by weight of plant-derived silicon water concentrate and 39.45 parts by weight of deionized water to a mixing tank and stir at 300 r / min for 10 min to obtain silicon water base solution. The system temperature is controlled at 37℃. Step 2: Add 5.00 parts by weight of glycerol, 3.00 parts by weight of 1,3-propanediol and 1.00 parts by weight of panthenol to the silicon aqueous base solution, and stir at 250 r / min for 20 min until completely dissolved to obtain aqueous phase mixture I; Step 3: Cool the aqueous phase mixture I to 31°C, add 0.20 parts by mass of silanized hyaluronic acid, 0.30 parts by mass of asiaticoside and 0.05 parts by mass of acetylated sodium hyaluronate, and stir at 200 r / min for 15 min until completely dissolved to obtain aqueous phase mixture II; Step 4: Cool the aqueous phase mixture II to 26°C, add 0.10 parts by mass of acetyl hexapeptide-8, and stir at 150 r / min for 10 min until uniformly dispersed to obtain aqueous phase mixture III; Step 5: Add 0.30 parts by weight of PEG-40 hydrogenated castor oil, 0.50 parts by weight of phenoxyethanol and 0.10 parts by weight of ethylhexylglycerin to aqueous phase mixture III, stir at 150 r / min for 8 min, filter through a 0.22 μm microporous membrane, and fill into a container to obtain silicone water that improves expression lines.

[0041] In this embodiment, the silicon concentration of the silicon-based aqueous solution was approximately 5198 ppm, and the final product pH value was approximately 6.8. After 28 days, the area of ​​expression lines decreased by 41.5%, the skin elasticity R2 value improved by 33.6%, the muscle contraction amplitude decreased by 32.8%, the stratum corneum moisture content increased by 43.8%, and the 12-hour cumulative penetration of acetyl hexapeptide-8 was 3.94 μg / cm².

[0042] Comparative Example 1: Raw material formula: 50.0 parts by weight of plant-derived silicon aqueous concentrate (silicon concentration 9300ppm), 39.75 parts by weight of deionized water (conductivity ≤5μS / cm), 5.00 parts by weight of glycerol (purity ≥99.5%), 3.00 parts by weight of 1,3-propanediol (purity ≥99.5%), 1.00 parts by weight of panthenol (purity ≥98%), 0.30 parts by weight of asiaticoside (purity ≥95%), 0.05 parts by weight of acetylated sodium hyaluronate (relative molecular mass 30-50kDa, degree of acetylation ≥60%), 0.30 parts by weight of PEG-40 hydrogenated castor oil (purity ≥99%), 0.50 parts by weight of phenoxyethanol (purity ≥99%), 0.10 parts by weight of ethylhexylglycerin (purity ≥98%), totaling 100.00 parts by weight.

[0043] Preparation process: Follow the steps in Example 1, skipping the steps of adding silanized hyaluronic acid and acetyl hexapeptide-8.

[0044] Comparative Example 1A: Raw material formulation: 50.0 parts by weight of plant-derived silicon aqueous concentrate (silicon concentration 9300ppm), 39.65 parts by weight of deionized water (conductivity ≤5μS / cm), 5.00 parts by weight of glycerol (purity ≥99.5%), 3.00 parts by weight of 1,3-propanediol (purity ≥99.5%), 1.00 parts by weight of panthenol (purity ≥98%), silanized hyaluronic acid (relative molecular mass 60-80kDa, silicon content... 0.10 parts by weight of 0.8% asiaticoside (purity ≥95%), 0.30 parts by weight of acetylated sodium hyaluronate (relative molecular mass 30-50kDa, degree of acetylation ≥60%), 0.05 parts by weight of PEG-40 hydrogenated castor oil (purity ≥99%), 0.30 parts by weight of phenoxyethanol (purity ≥99%), 0.50 parts by weight of ethylhexylglycerin (purity ≥98%), totaling 100.00 parts by weight.

[0045] This comparative example is based on the formulation of Example 1, except that acetyl hexapeptide-8 is removed, while silanized water and silanized hyaluronic acid are retained, and the remaining components are made up to 100 parts by weight with deionized water.

[0046] Preparation process: Follow the steps in Example 1, skipping the addition of acetyl hexapeptide-8 in step four.

[0047] The silicon concentration of this comparative silicon-based aqueous substrate was approximately 5190 ppm, and the final product pH was approximately 6.8. After 28 days, the area of ​​expression lines decreased by 16.5%, skin elasticity R2 value improved by 14.2%, muscle contraction amplitude decreased by 2.3%, and stratum corneum hydration increased by 34.2%. Because it did not contain acetyl hexapeptide-8, Franz diffusion cell transdermal absorption rate testing was not performed.

[0048] Comparative Example 2: Raw material formula: 89.60 parts by weight of deionized water (conductivity ≤5μS / cm), 5.00 parts by weight of glycerol (purity ≥99.5%), 3.00 parts by weight of 1,3-propanediol (purity ≥99.5%), 1.00 parts by weight of panthenol (purity ≥98%), 0.10 parts by weight of silanized hyaluronic acid (relative molecular mass 60-80kDa, silicon content 0.8%), and 0.30 parts by weight of asiaticoside (purity ≥95%). Parts by weight: 0.05 parts by weight of acetylated sodium hyaluronate (relative molecular mass 30-50 kDa, degree of acetylation ≥60%), 0.05 parts by weight of acetylated hexapeptide-8 (purity ≥97%), 0.30 parts by weight of PEG-40 hydrogenated castor oil (purity ≥99%), 0.50 parts by weight of phenoxyethanol (purity ≥99%), 0.10 parts by weight of ethylhexylglycerin (purity ≥98%), totaling 100.00 parts by weight.

[0049] Preparation process: Follow the steps in Example 1. In step one, only deionized water is used, and no plant-derived silicon water concentrate is added.

[0050] Comparative Example 3: Raw material formula: 50.0 parts by weight of plant-derived silicon aqueous concentrate (silicon concentration 9300ppm), 39.60 parts by weight of deionized water (conductivity ≤5μS / cm), 5.00 parts by weight of glycerol (purity ≥99.5%), 3.00 parts by weight of 1,3-propanediol (purity ≥99.5%), 1.00 parts by weight of panthenol (purity ≥98%), 0.10 parts by weight of sodium hyaluronate (relative molecular mass 60-80kDa), and asiaticoside (purity ≥99.5%). ≥95%) 0.30 parts by weight, acetylated sodium hyaluronate (relative molecular mass 30-50kDa, degree of acetylation ≥60%) 0.05 parts by weight, acetylated hexapeptide-8 (purity ≥97%) 0.05 parts by weight, PEG-40 hydrogenated castor oil (purity ≥99%) 0.30 parts by weight, phenoxyethanol (purity ≥99%) 0.50 parts by weight, ethylhexylglycerin (purity ≥98%) 0.10 parts by weight, totaling 100.00 parts by weight.

[0051] Preparation process: Follow the steps in Example 1, except that the silanized hyaluronic acid is replaced with an equal amount of sodium hyaluronate.

[0052] Comparative Example 4: Raw material formula: 15.0 parts by weight of plant-derived silicon aqueous concentrate (silicon concentration 9300ppm), 71.85 parts by weight of deionized water (conductivity ≤5μS / cm), 5.00 parts by weight of glycerol (purity ≥99.5%), 3.00 parts by weight of 1,3-propanediol (purity ≥99.5%), 3.00 parts by weight of collagen (relative molecular mass 3-5kDa), sodium hyaluronate (relative molecular mass 60-80kDa) 0.15 parts by weight of Da, 0.05 parts by weight of snake venom peptide (purity ≥95%), 0.05 parts by weight of cone snail toxin (purity ≥95%), 1.00 parts by weight of panthenol (purity ≥98%), 0.30 parts by weight of PEG-40 hydrogenated castor oil (purity ≥99%), 0.50 parts by weight of phenoxyethanol (purity ≥99%), 0.10 parts by weight of ethylhexylglycerin (purity ≥98%), totaling 100.00 parts by weight.

[0053] Preparation process: 15.0 parts by weight of plant-derived silicon aqueous concentrate and 71.85 parts by weight of deionized water are added to a mixing tank and stirred evenly. Glycerin, 1,3-propanediol, panthenol, collagen and sodium hyaluronate are added in sequence and stirred to dissolve. The mixture is cooled to below 30°C and snake venom peptide and cone snail toxin are added and stirred to disperse. Solubilizer and preservative are added and stirred. The mixture is filtered through a 0.22μm microporous membrane and then filled.

[0054] Comparative Example 5: Raw material formulation: 8.0 parts by weight of plant-derived silicon aqueous concentrate (silicon concentration 9300ppm), 74.50 parts by weight of deionized water (conductivity ≤5μS / cm), 5.00 parts by weight of glycerol (purity ≥99.5%), 3.00 parts by weight of 1,3-propanediol (purity ≥99.5%), 2.0 parts by weight of Kappa longifolia extract (solid content ≥50%), and Wakame seaweed extract (solid content ≥50%). 4.0 parts by weight, 1.5 parts by weight of nicotinamide (purity ≥99%), 0.10 parts by weight of lysine (purity ≥98%), 1.0 part by weight of lecithin (purity ≥95%), 0.30 parts by weight of PEG-40 hydrogenated castor oil (purity ≥99%), 0.50 parts by weight of phenoxyethanol (purity ≥99%), 0.10 parts by weight of ethylhexylglycerin (purity ≥98%), totaling 100.00 parts by weight.

[0055] Preparation process: 8.0 parts by weight of plant-derived silicon aqueous concentrate and 74.50 parts by weight of deionized water were added to a mixing tank and stirred evenly. The mixture was heated to 40°C and glycerol and 1,3-propanediol were added in sequence and stirred to dissolve. The mixture was cooled to 35°C and Kappa algae extract, wakame extract, nicotinamide, lysine and lecithin were added and stirred to dissolve. The mixture was cooled to below 30°C and solubilizer and preservative were added and stirred. The mixture was then filtered through a 0.22μm microporous membrane and filled into bottles.

[0056] Comparative Example 6: Raw material formulation: 6.0 parts by weight of silica sol (particle size 10-20nm, SiO2 content 30%), 83.60 parts by weight of deionized water (conductivity ≤5μS / cm), 5.00 parts by weight of glycerol (purity ≥99.5%), 3.00 parts by weight of 1,3-propanediol (purity ≥99.5%), 1.00 parts by weight of panthenol (purity ≥98%), 0.10 parts by weight of sodium hyaluronate (relative molecular mass 60-80kDa), and 0.10 parts by weight of asiaticoside (pure... 0.30 parts by weight of sodium acetylated hyaluronic acid (relative molecular mass 30-50 kDa, degree of acetylation ≥ 60%), 0.05 parts by weight of acetylated hexapeptide-8 (purity ≥ 97%), 0.30 parts by weight of PEG-40 hydrogenated castor oil (purity ≥ 99%), 0.50 parts by weight of phenoxyethanol (purity ≥ 99%), and 0.10 parts by weight of ethylhexylglycerin (purity ≥ 98%), totaling 100.00 parts by weight.

[0057] Preparation process: 6.0 parts by weight of silica sol and 83.60 parts by weight of deionized water were added to a mixing tank and stirred evenly. Subsequent steps were performed as in Example 1, except that the silanized hyaluronic acid was replaced with an equal amount of sodium hyaluronate.

[0058] Performance testing: Test Method 1: Reduction Rate of Expression Line Area. Ten female subjects aged 30-55 with prominent expression lines were selected in each group. The silicone water prepared in Examples 1-3 and Comparative Examples 1 and 1A-6 was applied to the facial expression line area twice daily, morning and evening, for 28 consecutive days. Facial images of the subjects were acquired before and after 28 days of use using a VISIA-CR facial image analyzer. The area of ​​expression lines was measured using Image-Pro Plus image analysis software, and the reduction rate of expression line area was calculated. Results are expressed as an average of 10 subjects.

[0059] Test Method 2: Skin Elasticity R2 Value. Using the Cutometer MPA580 skin elasticity tester, the skin elasticity R2 value of the facial cheek area was measured before and 28 days after use. The R2 value is the ratio of the rebound amount without negative pressure to the rebound amount with negative pressure. The closer the value is to 1, the better the elasticity. The improvement rate of R2 value was calculated, and the results are expressed as the average value of 10 people.

[0060] Test Method 3: Reduction Rate of Muscle Contraction Amplitude. Using a Myotrace 400 surface electromyography instrument, the RMS values ​​of the frontalis muscle during maximal voluntary contraction were measured before and 28 days after use. The reduction rate of muscle contraction amplitude was calculated, and the results are expressed as a mean of 10 subjects.

[0061] Test Method 4: Increase in Stratum Corneum Moisture Content. Using a Corneometer CM825 skin moisture analyzer, the stratum corneum moisture content of the cheek area was measured before and 28 days after use. The increase in moisture content was calculated, and the results are expressed as an average of 10 people.

[0062] Test Method 5: Transdermal Absorption Rate of Acetyl Hexapeptide-8. The Franz diffusion cell method was used, with isolated pig skin as the permeation barrier. Silicon-containing water prepared in Examples 1 to 3 and Comparative Examples 2 and 6 was added to the supply cell, and pH 7.4 phosphate buffer was added to the receiving cell. The magnetic stirring speed was 600 r / min, and the water bath circulation temperature was 32℃. Samples were taken after 12 hours, and the content of acetyl hexapeptide-8 in the receiving solution was determined by HPLC. The average value of 6 diffusion cells in each group was taken, and the cumulative permeation rate over 12 hours was calculated.

[0063] Test results: Table 1. Results of the test on the reduction rate of expression line area:

[0064] Test method: The test was conducted in accordance with Test Method 1. The VISIA-CR facial image analyzer and Image-Pro Plus image analysis software were used. The average value was taken for each group of 10 people.

[0065] Table 2 Results of the skin elasticity R2 value improvement rate test:

[0066] Test method: Performed according to Test Method 2, using a Cutometer MPA580 skin elasticity tester, with 10 people per group and the average value taken.

[0067] Table 3 Results of the muscle contraction amplitude reduction rate test:

[0068] Test method: The test was conducted in accordance with Test Method 3. The RMS value of the frontalis muscle during maximum voluntary contraction was measured using a Myotrace 400 surface electromyography instrument. The average value was taken for each group of 10 people.

[0069] Table 4. Test results of the increase rate of stratum corneum moisture content:

[0070] Test method: The test was conducted in accordance with Test Method 4. The Corneometer CM825 skin moisture meter was used to measure the moisture content of the stratum corneum in the cheek area. The average value was taken for each group of 10 people.

[0071] Table 5. Results of transdermal absorption rate test for acetyl hexapeptide-8:

[0072] Test method: Performed according to Test Method 5, using the Franz diffusion cell method, isolated porcine skin permeability barrier, and HPLC to determine the content of acetyl hexapeptide-8.

[0073] Results analysis: Examples 1 to 3 showed a reduction rate of 38.7% to 42.3% in expression line area, an improvement rate of 31.2% to 34.8% in skin elasticity R2 value, a reduction rate of 26.5% to 32.8% in muscle contraction amplitude, and an increase rate of 43.8% to 48.7% in stratum corneum moisture content. Example 2 had the highest addition amount of silicon-based aqueous concentrate (65.0 parts), the highest 12-hour cumulative penetration of acetyl hexapeptide-8 (4.52 μg / cm²), and the best reduction rates in expression line area and stratum corneum moisture content (42.3% and 48.7%, respectively). Example 3 had the highest addition amount of acetyl hexapeptide-8 (0.10 parts), the highest reduction rate in muscle contraction amplitude (32.8%), and a reduction rate of 41.5% in expression line area, second only to Example 2.

[0074] Comparative Example 1, lacking both acetyl hexapeptide-8 and silanized hyaluronic acid, showed a reduction of only 8.2% in the area of ​​expression lines and only 1.8% in the amplitude of muscle contraction, demonstrating that these two core active components make a crucial contribution to the improvement of expression lines. It should be noted that Comparative Example 1, lacking both core active components, is a combined control rather than a single-variable control. Its difference from Example 1 reflects the combined contribution of the two components. The individual contributions of each component can be demonstrated by Comparative Examples 1A, 2, and 3 respectively: Comparative Example 1A, by removing only acetyl hexapeptide-8, demonstrates the muscle-relaxing contribution of acetyl hexapeptide-8; Comparative Example 2, by removing silanized water, demonstrates the contribution of enhanced silanized water delivery; and Comparative Example 3, by replacing silanized hyaluronic acid with ordinary sodium hyaluronate, demonstrates the contribution of silanol group structure reconstruction.

[0075] Comparative Example 1A, based on the formulation of Example 1, only removed acetyl hexapeptide-8, retaining silanized water and silanized hyaluronic acid. The reduction rate of expression line area was 16.5%, and the reduction rate of muscle contraction amplitude was only 2.3%. Compared to Comparative Example 1 (which simultaneously lacked acetyl hexapeptide-8 and silanized hyaluronic acid, resulting in an 8.2% reduction rate of expression line area), Comparative Example 1A showed an 8.3 percentage point increase in expression line area reduction. This increase mainly stemmed from the contribution of the silanol groups in silanized hyaluronic acid to the structural reconstruction promoting collagen cross-linking.

[0076] Compared with Example 1 (reduction rate of expression line area of ​​38.7%), Comparative Example 1A decreased by 22.2 percentage points. This decrease is mainly attributed to the complete loss of the muscle relaxation pathway after the absence of acetyl hexapeptide-8. The continuous contraction of facial muscles not only directly produces dynamic lines, but also causes the structural reconstruction effect of silanized hyaluronic acid to be repeatedly damaged by mechanical stress and cannot be effectively consolidated. This further confirms the protective effect of the muscle relaxation pathway on the structural reconstruction pathway.

[0077] The reduction rate of muscle contraction amplitude in Comparative Example 1A was only 2.3%, which is close to 1.8% in Comparative Example 1 and much lower than 26.5% in Example 1. This demonstrates that, without acetyl hexapeptide-8, facial muscle contraction cannot be effectively inhibited regardless of whether silanized hyaluronic acid is present. Acetyl hexapeptide-8 is an irreplaceable component of the muscle relaxation pathway.

[0078] Compared with Comparative Example 2, Example 1 showed a 65% increase in the reduction rate of expression line area and a 27% increase in the reduction rate of muscle contraction amplitude, indicating that silicon-containing water as a delivery medium has a significant enhancing effect on improving expression lines. After removing silicon-containing water, the transdermal efficiency of acetyl hexapeptide-8 was greatly reduced (the cumulative permeation amount in 12 hours decreased from 3.87 to 1.23 μg / cm2, a reduction of 68%), and the muscle relaxation pathway was severely weakened.

[0079] Compared with Comparative Example 3, Example 1 showed a 75% increase in the reduction rate of expression line area and a 67% increase in the improvement rate of skin elasticity R2 value, indicating that the dermal structure reconstruction function of silanized hyaluronic acid is significantly better than that of ordinary sodium hyaluronate. The participation of silanol groups in collagen cross-linking is one of the key mechanisms for improving expression lines.

[0080] Although Comparative Example 3 contains silicon-enhanced water delivery and acetyl hexapeptide-8 muscle relaxants, it lacks the structural reconstruction pathway of silanol groups, which prevents the effective improvement of dermal collagen cross-linking density and limits the effect on improving expression lines.

[0081] Comparative Example 4 used silane water with snake venom peptides and cone snail toxins. The amount of silane water added was only 15 parts, and the reduction rate of expression lines area was only 19.8%, which was significantly lower than 38.7% in Example 1. This indicates that snake venom peptides and cone snail toxins lack sufficient penetration and delivery enhancement under the condition of only 15 parts of silane water added. In addition, this scheme does not contain silanized hyaluronic acid and lacks structural reconstruction pathways, so it cannot form a three-pathway synergistic system.

[0082] Comparative Example 5 showed a reduction rate of only 7.5% in the area of ​​expression lines and a reduction rate of only 3.4% in the amplitude of muscle contraction, indicating that the pure plant extract regimen without muscle-relaxing and structural reconstruction components had almost no effect on improving expression lines.

[0083] Comparative Example 6, which replaced silica water with ordinary silica sol and silanized hyaluronic acid with ordinary sodium hyaluronate, showed a 17.2% reduction in expression line area, a 56% decrease compared to Example 1. This indicates that the particle size of ordinary silica sol (10-20 nm) is much larger than the 0.4 nm water-soluble silicon particles in silica water, making it unable to achieve enhanced penetration through the stratum corneum gap. Furthermore, ordinary sodium hyaluronate lacks silanol groups and cannot participate in collagen cross-linking reconstruction. Both substitutions resulted in the failure of the three-pathway synergistic system. The cumulative penetration amount of Comparative Example 6 over 12 hours was only 1.85 μg / cm², a 52% decrease compared to Example 1, further demonstrating that ordinary silica sol does not possess the super-permeable delivery capability of silica water.

[0084] Synergistic effect verification: To verify the gain effect of the three-pathway synergy, the reduction rate of expression line area in Example 1 was compared and analyzed with the effect when each pathway was missing.

[0085] Comparative Example 1 (without acetyl hexapeptide-8 and silanized hyaluronic acid, but with silicone water) showed an 8.2% reduction in expression line area, reflecting the contribution of basic moisturizing and silicone replenishment solely from silicone water.

[0086] Comparative Example 2 (silicone-free water delivery medium, with acetyl hexapeptide-8 and silanized hyaluronic acid) showed a 23.4% reduction in expression line area, reflecting the contribution of the dual pathways of muscle relaxation and structural reconstruction when osmotic delivery enhancement is lacking.

[0087] Comparative Example 3 (ordinary sodium hyaluronate replacing silanized hyaluronic acid, with silanized water and acetyl hexapeptide-8) showed a 22.1% reduction in expression line area, reflecting the enhanced permeation and delivery of silanized water and the contribution of the acetyl hexapeptide-8 muscle relaxation pathway, but the structural reconstruction pathway was weakened due to the lack of silanol groups.

[0088] If the three pathways are independent and superimposed, the expected reduction rate of expression line area in Example 1 should be close to the sum of Comparative Examples 2 and 3 minus Comparative Example 1 (to avoid double-counting the contribution of silicon water), i.e., 23.4% plus 22.1% minus 8.2% equals 37.3%. However, the actual reduction rate of expression line area in Example 1 was 38.7%, which is 1.4 percentage points higher than the expected value of 37.3% in the additive model. The effect of the synergy of the three pathways exceeds the sum of the individual effects of each pathway.

[0089] The synergistic mechanism is as follows: the super-permeable delivery of silicon water increases the transdermal efficiency of acetyl hexapeptide-8 by more than 3 times; the enhanced muscle relaxation effect prevents the reconstruction of dermal structure from being damaged by repeated mechanical stress; the improved dermal microenvironment after structural reconstruction provides a more favorable path for the penetration and diffusion of silicon particles; the three pathways form a positive feedback loop, producing a gain that exceeds the sum of the individual effects of each pathway.

[0090] Example 1 showed a 65% improvement over Comparative Example 2 and a 75% improvement over Comparative Example 3.

[0091] The above data shows that Comparative Example 1A, which lacks only one component, acetyl hexapeptide-8, reduced the area of ​​expression lines from 38.7% in Example 1 to 16.5%, a decrease of 57%, further confirming that the absence of any pathway leads to a significant decrease in the improvement effect of expression lines.

[0092] The preferred embodiments of the present invention disclosed above are merely illustrative of the invention. These preferred embodiments do not exhaustively describe all details, nor do they limit the invention to the specific implementations described. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of the invention, thereby enabling those skilled in the art to better understand and utilize the invention.

Claims

1. A silicon-based water for improving expression lines, characterized in that, The product comprises the following components by mass: 40-70 parts of plant-derived silicon aqueous concentrate, wherein the silicon concentration in the plant-derived silicon aqueous concentrate is 8000-12000 ppm, wherein the silicon exists in the form of monomolecular orthosilicic acid or its oligomers, and the hydrated particle size is ≤0.4 nm; and 0.005-0.1 parts of acetyl hexapeptide-8. 0.01-0.5 parts of silanized hyaluronic acid, wherein the relative molecular mass of the silanized hyaluronic acid is 50-100 kDa and the silicon content is ≥0.5%; 8-12 parts of moisturizer; Panthenol 0.5-2 parts; Centella asiatica 0.1-0.5 parts; Acetylated sodium hyaluronate 0.01-0.1 parts; Solubilizer 0.1-0.5 parts; Preservative 0.35-1 parts; Deionized water balance.

2. The silicon-based water for improving expression lines as described in claim 1, characterized in that, The plant-derived silicon water concentrate is obtained by high-temperature alkaline extraction of barley husks or rice husks, with a silicon concentration of 9000-10000 ppm and a pH value of 7.0-8.

5.

3. The silicon-based water for improving expression lines as described in claim 1, characterized in that, The acetyl hexapeptide-8 has a purity of ≥97% and is added in an amount of 0.01-0.05 parts by mass.

4. The silicon-based water for improving expression lines as described in claim 1, characterized in that, The degree of substitution of silanol groups in the silanized hyaluronic acid is 0.5-2.0%, and its relative molecular mass is 60-80 kDa.

5. The silicon-based water for improving expression lines as described in claim 1, characterized in that, The moisturizer is composed of glycerin and 1,3-propanediol, with a mass ratio of glycerin to 1,3-propanediol of 1:0.4 to 1:0.

8.

6. The silicon-based water for improving expression lines as described in claim 1, characterized in that, The solubilizer is PEG-40 hydrogenated castor oil, and the preservative is composed of phenoxyethanol and ethylhexylglycerin, with a mass ratio of phenoxyethanol to ethylhexylglycerin of 5:

1.

7. A method for preparing silicon-based water for improving expression lines as described in any one of claims 1-6, characterized in that, Includes the following steps: Step 1: Mix the plant-derived silicon water concentrate with deionized water and stir until homogeneous to obtain silicon water base solution. Control the system temperature at 35-40℃. Step 2: Add humectant and panthenol to the silicon aqueous base solution, and stir at 200-300 r / min for 15-25 min until completely dissolved to obtain aqueous phase mixture I; Step 3: Cool the aqueous phase mixture I to 30-35℃, add silanized hyaluronic acid, asiaticoside and acetylated sodium hyaluronate, and stir at 150-250 r / min for 10-20 min until completely dissolved to obtain aqueous phase mixture II; Step 4: Cool the aqueous phase mixture II to 25-28℃, add acetyl hexapeptide-8, and stir at 100-200 r / min for 5-10 min until uniformly dispersed to obtain aqueous phase mixture III; Step 5: Add solubilizer and preservative to aqueous mixture III, stir at 100-200 r / min for 5-10 min, filter through a 0.22 μm microporous membrane, and fill into a container to obtain silicon water that improves expression lines.

8. The method for preparing silicon-based water for improving expression lines as described in claim 7, characterized in that, In step one, the silicon concentration in the silicon-based aqueous solution is 5000-9000 ppm, and in step four, the addition temperature of acetyl hexapeptide-8 is controlled below 28℃.

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