Application of ranae chensinensis seed and extract thereof in preparing animal hair improvement product

Abstract

The present application relates to the application of forest frog seeds and extracts thereof in the preparation of animal hair improvement products, and belongs to the field of animal health care and animal feed technology. The present application has the beneficial effect of disclosing the use of forest frog seeds and forest frog seed extracts for improving animal hair. The forest frog seed powder is directly pulverized from forest frog seeds, and the oil of the forest frog seeds is obtained by supercritical extraction or cold pressing method. The active ingredients are fully retained, and the effect is significant. After oral administration to animals, the forest frog seeds and extracts thereof not only promote hair growth and increase density, but also improve gloss, softness and toughness, and reduce hair breakage and shedding. Animal experiments show that continuous oral administration of forest frog seeds and extracts thereof can improve hair growth, hair gloss, hair density and enhance the antioxidant capacity of the skin, providing sufficient scientific basis for the application of pets and other animals.

Description

Technical Field

[0001] This invention belongs to the field of animal health and animal feed technology, specifically relating to the application of forest frog seeds and their extracts in the preparation of products that improve animal hair. Background Technology

[0002] An animal's coat condition is a crucial indicator of its health. For pets (dogs, cats, etc.), glossy, smooth, and thick fur not only enhances aesthetics but also often signifies good nutrition and healthy skin. For fur-producing animals (minks, rabbits, etc.), coat (fur) quality directly impacts economic value. However, influenced by genetic factors, malnutrition, environmental stress, skin diseases, oxidative stress, and other factors, many animals suffer from dry, brittle, and brittle fur, severe hair loss, and slow hair growth, significantly affecting their health and appearance. Currently, products on the market for improving animal coat conditioner are mainly divided into two categories: topical and oral products. Topical products include pet shampoos, conditioners, and sprays. Their mechanism of action primarily involves cleaning the skin, replenishing surface oils, and temporarily increasing coat smoothness. However, their effects are relatively superficial, and the results are difficult to maintain after discontinuation. Furthermore, they cannot fundamentally improve the nutrient supply to hair follicles and their internal metabolic environment. Oral products, including nutritional supplements or functional diets containing lecithin, multivitamins, and trace elements, offer the advantage of regulating the hair follicle growth cycle, improving skin lipid metabolism, and enhancing antioxidant capacity from the inside out, thus achieving a lasting improvement in hair quality. However, existing oral products still have the following shortcomings: First, the ingredients are relatively simple or lack synergy, resulting in limited hair improvement effects, usually only targeting one aspect; second, some products have poor palatability, are prone to oxidation and rancidity, and long-term feeding may lead to abnormal body odor in animals, affecting pets' willingness to eat and compliance; third, some products have insufficient dosage or low bioavailability of active ingredients, resulting in insignificant actual effects. Therefore, developing a new type of oral product that is low-cost, natural and safe, palatable, and can comprehensively improve animal hair quality from multiple dimensions has significant market value and application prospects.

[0003] Forest frog eggs are the egg nuclei of female forest frogs, traditionally considered a byproduct and discarded, resulting in low resource utilization. Forest frog eggs have been found to have effects such as anti-fatigue, weight loss, and lowering blood lipids, but their application in improving animal coat health has not yet been reported. Summary of the Invention

[0004] This invention provides the application of frog seeds and their extracts in the preparation of products to improve animal hair. The purpose is to provide a new use for frog seeds and their extracts in the preparation of products to improve animal hair, opening up a new way for the high-value utilization of waste frog seeds, and also providing a safe and effective new solution for improving animal hair.

[0005] The technical solution adopted in this invention is: Application of forest frog seeds in the preparation of products that improve animal hair.

[0006] The application of forest frog seed extract in the preparation of products that improve animal hair, wherein the forest frog seed extract is an extract rich in fat-soluble active ingredients.

[0007] The frog seed extract is an oil obtained by supercritical CO2 extraction or cold pressing, wherein: The conditions for the supercritical CO2 extraction method are: extraction pressure 25~40 MPa, extraction temperature 35~55℃, separation pressure 5~10 MPa, and separation temperature 30~45℃. The cold pressing method involves mechanically pressing the frog eggs at a temperature below 60°C and then centrifuging them to obtain the oil.

[0008] The improvement of animal hair includes promoting hair growth and improving hair quality, wherein: (1) Promoting hair growth includes: (a) Increase hair density; (b) Increase hair length; (2) Improving hair quality includes: (a) Improves hair shine; (b) Increases hair smoothness and improves dry, rough, or brittle hair; (c) Increases VEGF content in the skin and decreases serum TNF-α and IL-6 levels.

[0009] The animal in question is a mammal.

[0010] The mammals are selected from: dogs, cats, rabbits, mice, minks, horses, or fur-bearing animals.

[0011] The dosage forms of the products are selected from: powder, granules, tablets, capsules, oral liquids, soft chewables, nutritional pastes, daily rations, snacks, nutritional supplements, freeze-dried blocks, freeze-dried snacks, gels, liquid additives, emulsions, suspensions, premixes, additive premixes, pet food, staple food, wet food, canned food, cat treats, freeze-dried pet food, or pet chews.

[0012] The frog eggs and their extracts constitute 2% to 90% of the product by mass.

[0013] A product for improving animal hair contains an effective amount of forest frog seeds and their extracts, as well as an animal-acceptable carrier or excipient.

[0014] The products are veterinary drugs, animal health products, animal nutritional supplements, animal foods, animal feed additives, or animal feed.

[0015] The present invention has the following beneficial effects: This study marks the first public disclosure of the use of forest frog seeds and their extracts to improve animal coat health. Forest frog seed powder, obtained through direct grinding of the seeds, and especially oils obtained via supercritical fluid extraction or cold pressing, retain the active ingredients effectively, resulting in significant effects. Oral administration to animals showed that forest frog seeds and their extracts not only promoted hair growth and increased density but also improved shine, smoothness, and resilience, while reducing breakage and hair loss. Animal studies demonstrated that continuous oral administration of forest frog seeds and their extracts improved hair growth, shine, and density, and enhanced skin's antioxidant capacity, providing substantial scientific evidence for their application in pets and other animals. Attached Figure Description

[0016] Figure 1 This is a scoring chart of the new hair growth status in mice according to an embodiment of the present invention; Figure 2 This is a graph showing the length of newly grown hair in mice according to an embodiment of the present invention; Figure 3 This is a graph showing the brightness and smoothness scores of newly grown fur in mice according to an embodiment of the present invention; Figure 4 These are epidermal growth factor diagrams for each group in the embodiments of the present invention; Figure 5 These are TNF-α factor diagrams for each group in the embodiments of the present invention; Figure 6 These are IL-6 factor diagrams for each group in the embodiments of the present invention. Detailed Implementation Example 1

[0017] 1000g of dried frog eggs were crushed and passed through a 40-mesh sieve, then added to a supercritical CO2 extraction vessel. The extraction pressure was set to 36 MPa, the extraction temperature to 50℃, the separation pressure to 8 MPa, the separation temperature to 38℃, the CO2 flow rate to 80 L / h, and the extraction time to 3 hours. The extract in the separation vessel was collected, and impurities were separated by centrifugation to obtain a pale yellow, transparent oily liquid, which is frog egg oil, with a yield of 10.5%. Example 2

[0018] Take 1000g of dried frog eggs, crush them through a 40-mesh sieve, and put them into a supercritical CO2 extraction vessel. Set the extraction pressure to 25MPa, extraction temperature to 35℃, separation pressure to 5MPa, separation temperature to 30℃, CO2 flow rate to 80 L / h, and extraction time to 2.5 hours. Collect the extract in the separation vessel, and centrifuge to separate impurities to obtain a pale yellow, transparent oily liquid, which is frog egg oil. Example 3

[0019] Take 1000g of dried forest frog seeds, crush them through a 40-mesh sieve, and put them into a supercritical CO2 extraction vessel. Set the extraction pressure to 40MPa, extraction temperature to 55℃, separation pressure to 10MPa, separation temperature to 45℃, CO2 flow rate to 80 L / h, and extraction time to 3.5 hours. Collect the extract in the separation vessel, and centrifuge to separate impurities, obtaining a pale yellow, transparent oily liquid, which is forest frog seed oil. Example 4

[0020] 1000g of fresh frog eggs were dried at 50±5℃ until the moisture content was ≤8%. The dried frog eggs were then put into a low-temperature screw press, and the pressing temperature was controlled below 60℃ for mechanical pressing to obtain crude oil. The crude oil was allowed to settle, centrifuged (4000 rpm, 15 minutes) to remove solid residues, and then filtered through a plate and frame filter to obtain yellow frog egg oil with a yield of 7.2%. Example 5

[0021] Take 1000g of fresh frog eggs and dry them at 50±5℃ until the moisture content is ≤7%. Put the dried frog eggs into a low-temperature screw press, and mechanically press them at a temperature below 55℃ to obtain crude oil. After settling and centrifugation (3500 rpm, 25 minutes) to remove solid residues, the crude oil is then filtered through a plate and frame filter to obtain yellow frog egg oil. Example 6

[0022] Take 1000g of fresh frog eggs and dry them at 50±5℃ until the moisture content is ≤9%. Put the dried frog eggs into a low-temperature screw press, and mechanically press them at a temperature below 50℃ to obtain crude oil. After settling and centrifugation (4500 rpm, 20 minutes) to remove solid residues, the crude oil is filtered through a plate and frame filter to obtain yellow frog egg oil. Example 7

[0023] Take 5.0% of the frog seed oil, 15.0% of gelatin, 12.0% of glycerin, 18.0% of starch, 20.0% of chicken hydrolysate, 3.0% of yeast extract, 2.0% of lecithin, 0.5% of vitamin E, 0.3% of potassium sorbate, and the remainder purified water prepared in Example 1. Mix the gelatin, glycerin, and purified water, heat to 65°C and stir until dissolved and homogeneous; add the frog seed oil and lecithin, and emulsify using a high-shear emulsifier for 10 minutes; add the chicken hydrolysate, yeast extract, starch, vitamin E, and potassium sorbate, and stir until homogeneous; pour the mixture into a soft chewable mold, cool to room temperature to solidify; after demolding, dry at a low temperature below 40°C until the moisture content is ≤12%, and then package to obtain the final product. Example 8

[0024] Take 8.0% of the frog seed oil, 45.0% of maltose syrup, 8.0% of corn oil, 12.0% of milk powder, 4.0% of lecithin, 0.5% of taurine, 1.0% of vitamin B complex premix, 0.5% of xanthan gum, 0.2% of sodium benzoate, and the remainder purified water prepared in Example 4. Mix the maltose syrup, purified water, and xanthan gum, heat to 55°C and stir to dissolve; add the frog seed oil, corn oil, and lecithin, and homogenize and emulsify for 15 minutes; cool to below 40°C, add the milk powder, taurine, vitamin B complex, and sodium benzoate, and stir evenly; fill into tubes and seal to obtain the final product. The nutritional paste prepared in this example contains 8.0% frog seed oil. Example 9

[0025] Take 2.0% of frog seed oil, 0.2% of vitamin E, 0.3% of chicken fat prepared in Example 1, and 97.5% of commercially available extruded dog food. Mix the frog seed oil, chicken fat, and vitamin E evenly and heat to 35-40°C. After the food is dispensed but before cooling, at approximately 45°C, spray the oil mixture evenly onto the surface of the food using a spraying device. Dry with cold air and cool to room temperature, then package. The pet food prepared in this example contains 2.0% frog seed oil and can be directly fed as a daily functional feed. Example 10

[0026] Take 90.0% of the frog seed oil, 6.0% of the lecithin, 2.0% of the vitamin E, and 2.0% of the beeswax prepared in Example 4. Melt the beeswax at 60°C, add the frog seed oil, lecithin, and vitamin E, and stir until homogeneous. Separately, mix gelatin, glycerin, and purified water in a ratio of 10:4:10, heat to 70°C, stir to dissolve, and degas under vacuum to obtain a capsule shell. Fill the contents into soft capsule shells and seal. Each capsule contains 500mg of the contents. Small dogs and cats should take one capsule daily, and medium to large dogs should take two to three capsules daily, either swallowed directly or mixed into food after puncturing the capsule. Example 11

[0027] The following ingredients are mixed and extruded: 10.0% frog eggs, 20.0% chicken meal, 20.0% corn meal, 20.0% rice meal, 8.0% chicken fat, 2.0% lecithin, 1.5% compound vitamin premix, 1.5% compound mineral premix, and the remainder water. They are then fed directly as a daily diet. Example 12

[0028] The diet consists of 12.0% frog egg powder, 40.0% alfalfa meal, 20.0% oat flour, 10.0% flaxseed meal, 5.0% lecithin, 3.0% vitamin premix, 2.0% mineral premix, 0.5% yeast selenium, and the balance maltodextrin. All ingredients are sieved through a 60-mesh sieve and mixed evenly according to the specified proportions. The mixture is then packaged into moisture-proof aluminum foil bags to obtain the animal coat improvement feed / ration. Example 13

[0029] Frog seed powder 15.0%, grain powder (millet powder, oat powder, and barley powder mixed in a 1:1:1 ratio) 45.0%, honey 8.0%, peanut butter 5.0%, vegetable powder 5.0%, lecithin 2.0%, vitamin E 0.5%, and the balance water. Used for preparing pet treat bars. Example 14

[0030] Forty healthy male C57BL / 6 mice (weighing 20-22g) aged 6-8 weeks were randomly divided into four groups (n=10): control group (CON, fed standard diet and administered saline by gavage), frog seed group (LWF, fed diet containing 20% ​​frog seed powder), frog seed supercritical extract group (CLJ, fed regular diet and administered 300mg / kg / d by gavage), and frog seed cold-pressed extract group (LYF, fed standard diet and administered 300mg / kg / d by gavage). Two weeks later, the back hair of all mice in each group was shaved with an electric razor, and then a hair removal cream was applied to an area of ​​approximately 4cm × 3cm. The medication was continued for three weeks after hair removal. At weeks 1, 2, and 3 post-hair removal, the new hair was scored based on hair coverage and length (0 points for no hair, 3 points for fully grown hair). Shininess and smoothness were also scored (0 points for poor, 5 points for best). After the experiment, blood was collected from the eye sockets and the mice were euthanized. Skin from the hairless area was taken, and hair was scraped from an equal area of ​​skin using a punch, weighed, and measured in length. Skin tissue was taken separately, homogenized, centrifuged, and the supernatant was collected. The VEGF content in the skin and the levels of TNF-α and IL-6 in the serum were detected by ELISA.

[0031] Appendix Figure 1 As shown, the hair growth scores of mice in each experimental group (LWF, LYF, CLJ) were higher than those in the control group (CON), and the scores gradually increased over time. On day 14, the scores of the LWF, LYF, and CLJ groups were significantly higher than those of the CON group, indicating that *Rhizophora stylosa* seeds and their extracts can effectively promote early hair regeneration and coverage. (See attached image.) Figure 2 It can be seen that, compared with the CON group, the length of newly grown hair in the LYF and CLJ groups was significantly increased. (See attached image) Figure 3 The results showed that, compared with the CON group, the LWF, LYF, and CLJ groups all exhibited improved scores in the brightness and smoothness of newly formed fur. (See attached image) Figure 4 The results showed that the VEGF levels in the LWF, SJX, and LYF groups were all higher than those in the CON group. The VEGF level in the CLJ group was significantly higher than that in the CON group. Figure 5 and attached Figure 6 The results showed that the levels of skin inflammatory factors TNF-α and IL-6 were reduced in mice treated with frog seeds and their extracts, with the most significant reduction observed in the CLJ group. These experimental results indicate that frog seeds and their extracts can be used to improve animal hair quality.

Claims

1. Application of forest frog seeds in the preparation of products that improve animal hair.

2. The application of forest frog seed extract in the preparation of products for improving animal hair, characterized in that: The frog seed extract is an extract rich in fat-soluble active ingredients.

3. The application according to claim 2, characterized in that: The frog seed extract is an oil obtained by supercritical CO2 extraction or cold pressing, wherein: The conditions for the supercritical CO2 extraction method are: extraction pressure 25~40 MPa, extraction temperature 35~55℃, separation pressure 5~10 MPa, and separation temperature 30~45℃. The cold pressing method involves mechanically pressing the frog eggs at a temperature below 60°C and then centrifuging them to obtain the oil.

4. The application according to any one of claims 1-3, characterized in that, The improvement of animal hair includes promoting hair growth and improving hair quality, wherein: (1) Promoting hair growth includes: (a) Increase hair density; (b) Increase hair length; (2) Improving hair quality includes: (a) Improves hair shine; (b) Increases hair smoothness and improves dry, rough, or brittle hair; (c) Increases VEGF content in the skin and decreases serum TNF-α and IL-6 levels.

5. The application according to any one of claims 1-4, characterized in that, The animal in question is a mammal.

6. The application according to claim 5, characterized in that, The mammals are selected from: dogs, cats, rabbits, mice, minks, horses, or fur-bearing animals.

7. The application according to claim 1, characterized in that, The dosage forms of the products are selected from: powder, granules, tablets, capsules, oral liquids, soft chewables, nutritional pastes, daily rations, snacks, nutritional supplements, freeze-dried blocks, freeze-dried snacks, gels, liquid additives, emulsions, suspensions, premixes, additive premixes, pet food, staple food, wet food, canned food, cat treats, freeze-dried pet food, or pet chews.

8. The application according to any one of claims 1-7, characterized in that, The frog eggs and their extracts constitute 2% to 90% of the product by mass.

9. A product for improving animal hair, characterized in that, It contains an effective amount of forest frog seeds and their extracts, as well as animal-acceptable carriers or excipients.

10. The product according to claim 9, characterized in that, The products are veterinary drugs, animal health products, animal nutritional supplements, animal foods, animal feed additives, or animal feed.

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