Alcoholism relieving and liver protecting product and application thereof

Abstract

The application provides a product for relieving alcoholism and protecting liver and application thereof, and belongs to the technical field of functional food. The application provides a product for relieving alcoholism and protecting liver, which comprises the following components in volume fraction: sea buckthorn extract 8-12 parts, cooked liquid of dog fig 16-20 parts, cooked liquid of gynostemma pentaphyllum 8-12 parts, hawthorn extract 14-18 parts, cooked liquid of indigofera 4-8 parts, and cooked liquid of wedelia prostrata 1-3 parts. The raw materials used in the application have the effects of relieving alcoholism, protecting liver, invigorating spleen and benefiting stomach, and have an auxiliary protection effect on alcoholic liver injury, and have a wide application range. The application compares the effects of the products for relieving alcoholism and protecting liver prepared from different raw material components through examples, and the results show that the components can significantly improve the activity of ethanol dehydrogenase, enhance the cell antioxidant capacity, and effectively relieve alcohol-induced cell damage.

Description

Technical Field

[0001] This invention belongs to the field of functional food technology, specifically relating to a product for relieving hangovers and protecting the liver, and its application. Background Technology

[0002] my country has a long history of winemaking, and alcoholic beverages are widely integrated into daily life. However, excessive drinking seriously harms human health. Alcohol is primarily metabolized in the liver, and long-term heavy drinking can gradually lead to alcoholic liver disease, cirrhosis, and even liver cancer. It also easily causes complications such as acute cholecystitis and pancreatitis. Alcohol disrupts lipid metabolism, raising cholesterol and triglycerides, increasing blood pressure and heart rate, and increasing the risk of stroke. Long-term intake can lead to myocardial fatty degeneration and deficiencies in various nutrients. Furthermore, alcohol strongly irritates the digestive tract mucosa, easily causing mucosal congestion and erosion, and inducing esophagitis, gastritis, and peptic ulcers.

[0003] Although there are many hangover relief products on the market, they have the drawback of weak liver protection effects, making it difficult to meet the needs of both hangover relief and liver protection. Summary of the Invention

[0004] In view of this, the present invention provides a product for relieving hangovers and protecting the liver, which can effectively improve the activity of alcohol dehydrogenase, enhance the antioxidant capacity of cells, and alleviate alcohol-induced cell damage.

[0005] To achieve the above objectives, the present invention provides the following technical solution: This invention provides a product for relieving hangovers and protecting the liver, comprising the following components in parts by volume: 8-12 parts of sea buckthorn extract, 16-20 parts of Japanese raisin tree decoction, 8-12 parts of gynostemma pentaphyllum decoction, 14-18 parts of hawthorn extract, 4-8 parts of akebia quinata decoction, and 1-3 parts of lysimachia christinae decoction.

[0006] Preferably, the sea buckthorn extract includes sea buckthorn juice; The hawthorn extract is obtained by boiling hawthorn pulp and water and then separating the liquid phase.

[0007] Preferably, the sea buckthorn extract is obtained by mixing sea buckthorn juice with water and then separating the liquid phase; the mass ratio of sea buckthorn juice to water is 1:(1~2).

[0008] The hawthorn extract is obtained by mixing hawthorn pulp with water, boiling it, and then keeping it at a constant temperature. After keeping it at a constant temperature, the liquid phase is separated to obtain the hawthorn extract. The temperature for keeping it at a constant temperature is 45~55℃ and the time is 20~40min.

[0009] The decoction of Japanese raisin tree fruit is obtained by boiling Japanese raisin tree fruit and water, separating the liquid phase to obtain the filtrate; the boiling is repeated twice, and the three filtrates are combined to obtain the decoction of Japanese raisin tree fruit; the mass ratio of Japanese raisin tree fruit to water is 1:(10~15).

[0010] The Gynostemma pentaphyllum decoction is obtained by decocting Gynostemma pentaphyllum and water, separating the liquid phase to obtain the filtrate; the decoction is repeated twice, and the three filtrates are combined to obtain the Gynostemma pentaphyllum decoction; the mass ratio of Gynostemma pentaphyllum to water is 1:(10~15).

[0011] The decoction of Akebia quinata is obtained by boiling Akebia quinata and water, separating the liquid phase to obtain the filtrate; the boiling is repeated twice, and the filtrates from the three times are combined to obtain the decoction of Akebia quinata; the mass ratio of Akebia quinata to water is 1:(10~15).

[0012] The decoction of *Lysimachia christinae* is obtained by decocting *Lysimachia christinae* and water, separating the liquid phase, and then repeating the decoction once more, combining the two filtrates to obtain the decoction. The mass ratio of *Lysimachia christinae* to water is 1:(8~12).

[0013] Preferably, it also includes at least one of the following components: thickener, preservative and flavoring agent.

[0014] The present invention provides a method for preparing the product, comprising: mixing the components in the product to obtain the product.

[0015] This invention provides an application of the product in improving the activity of alcohol dehydrogenase.

[0016] This invention provides an application of the product in improving antioxidant properties.

[0017] This invention provides an application of the product in alleviating alcohol-induced cellular damage.

[0018] This invention provides an application of the product in the preparation of products for relieving hangovers and / or protecting the liver.

[0019] Compared with the prior art, the present invention has the following advantages: This invention provides a product for relieving hangovers and protecting the liver, comprising the following components in parts by volume: 8-12 parts sea buckthorn extract, 16-20 parts decoction of Japanese raisin tree fruit, 8-12 parts decoction of Gynostemma pentaphyllum, 14-18 parts hawthorn extract, 4-8 parts decoction of Akebia quinata, and 1-3 parts decoction of Lysimachia christinae. In this invention, the raw materials work synergistically to effectively relieve hangovers and protect the liver. This invention compares the effects of hangover-relieving and liver-protecting products prepared from different raw material components through examples. The results show that the product with the stated components can effectively increase alcohol dehydrogenase activity, enhance cellular antioxidant capacity, and alleviate alcohol-induced cellular damage. Attached Figure Description

[0020] Figure 1 This is a graph showing the results of in vitro alcohol detoxification activity assay; Figure 2 This is a graph showing the results of in vitro antioxidant assays; Figure 3Figure showing the effect of the product on the viability of HepG2 cells; Figure 4 Figure showing the results of establishing an ethanol-induced alcoholic injury model in HepG2 cells; Figure 5 The figure shows the effect of the product on the viability of alcohol-induced HepG2 cells. Detailed Implementation

[0021] This invention provides a product for relieving hangovers and protecting the liver, comprising the following components in parts by volume: 8-12 parts of sea buckthorn extract, 16-20 parts of Japanese raisin tree decoction, 8-12 parts of gynostemma pentaphyllum decoction, 14-18 parts of hawthorn extract, 4-8 parts of akebia quinata decoction, and 1-3 parts of lysimachia christinae decoction.

[0022] In this invention, the product is preferably diluted with water to a volume of 100 parts. The components of this invention work synergistically to effectively alleviate hangovers and protect the liver. This invention compares the effects of hangover-relieving and liver-protecting products prepared from different raw material components through examples. The results show that the components can effectively increase alcohol dehydrogenase activity, enhance cellular antioxidant capacity, and alleviate alcohol-induced cellular damage. The Hovenia dulcis fruit (… Hovenia dulcis ), Gynostemma pentaphyllum ( Gynostemma pentaphyllum (Thunb.)Makino.), August fern (Akebiae Fructus), and field yellow ( Hypericum japonicum Thunb. is preferably made from dried medicinal materials. In this embodiment of the invention, Akebia quinata is selected from the woody plant Akebia quinata, Latin name... Akebia quinata (Thunb.) Decne.

[0023] In this invention, the sea buckthorn extract is preferably 12 parts. The sea buckthorn extract preferably includes sea buckthorn juice. The preparation method of the sea buckthorn extract preferably involves mixing sea buckthorn juice with water and then separating the liquid phase. This method facilitates the extraction of the effective components of sea buckthorn, allowing them to synergistically exert their hangover-relieving and liver-protecting effects with other components. The mass ratio of sea buckthorn juice to water is preferably 1:(1~2), more preferably 1:1. This ratio facilitates the extraction of the effective components of sea buckthorn and adjusts the concentration of the components, thereby better synergizing with other components to exert their hangover-relieving and liver-protecting effects.

[0024] In this invention, the hawthorn extract is preferably 16 parts. The hawthorn extract is preferably obtained by boiling hawthorn pulp and water and then separating the liquid phase. In this invention, the preparation method of the hawthorn extract preferably includes mixing hawthorn pulp and water, boiling, and then keeping warm. After keeping warm, the liquid phase is separated to obtain the hawthorn extract. This method is more conducive to promoting the dissolution of active ingredients in hawthorn, allowing them to synergistically exert their effects of relieving hangovers and protecting the liver with other components. The mass ratio of hawthorn pulp to water is preferably 1:(8~15), more preferably 1:(9~12), and even more preferably 1:10. The boiling temperature is preferably 95~105℃, more preferably 100℃. The boiling time is preferably 3~10 min, more preferably 4~7 min, and even more preferably 5 min. The keeping temperature is preferably 45~55℃, more preferably 48~52℃, and preferably 50℃. The keeping time is preferably 20~40 min, more preferably 25~35 min, and even more preferably 30 min. The aforementioned heat preservation method is more conducive to promoting the dissolution of active ingredients in hawthorn, which can work synergistically with other components to achieve the effects of relieving hangovers and protecting the liver.

[0025] In this invention, the decoction of Hovenia dulcis seeds is preferably 18 parts. Preferably, the decoction is prepared by decocting Hovenia dulcis seeds and water, separating the liquid phase to obtain a filtrate; the decoction is repeated twice, and the three filtrates are combined to obtain the decoction. The mass ratio of Hovenia dulcis seeds to water is preferably 1:(10-15), more preferably 1:(11-13), and even more preferably 1:12. This method is more conducive to promoting the dissolution of active ingredients in Hovenia dulcis seeds, allowing them to synergistically exert their effects of relieving hangovers and protecting the liver with other components.

[0026] In this invention, the preferred amount of the Gynostemma pentaphyllum decoction is 8 parts. Preferably, the Gynostemma pentaphyllum decoction is prepared by decocting Gynostemma pentaphyllum and water, separating the liquid phases to obtain a filtrate; the decoction is repeated twice, and the three filtrates are combined to obtain the Gynostemma pentaphyllum decoction. The preferred mass ratio of Gynostemma pentaphyllum to water is 1:(10~15), more preferably 1:(11~13), and even more preferably 1:12. This method is more conducive to promoting the dissolution of active ingredients in Gynostemma pentaphyllum, allowing it to synergistically exert its effects of detoxification and liver protection with other components.

[0027] In this invention, the Akebia quinata decoction is preferably composed of 4 parts. Preferably, the Akebia quinata decoction is prepared by decocting Akebia quinata and water, separating the liquid phases to obtain a filtrate; the decoction is repeated twice, and the three filtrates are combined to obtain the Akebia quinata decoction. The mass ratio of Akebia quinata to water is preferably 1:(10~15), more preferably 1:(11~13), and even more preferably 1:12. This method is more conducive to promoting the dissolution of active ingredients in Akebia quinata, allowing it to synergistically exert its hangover-relieving and liver-protecting effects with other components.

[0028] In this invention, the decoction of *Lysimachia christinae* is preferably 1 part. Preferably, the decoction is prepared by decocting *Lysimachia christinae* and water, separating the liquid phases to obtain a filtrate; the decoction is repeated once more, and the two filtrates are combined to obtain the decoction. The mass ratio of *Lysimachia christinae* to water is preferably 1:(8~12), more preferably 1:(8~11), and even more preferably 1:10. This method is more conducive to promoting the dissolution of active ingredients in *Lysimachia christinae*, reducing the bitterness of *Lysimachia christinae*, improving sensory evaluation, and better synergistically exerting the effects of alcohol detoxification and liver protection with other components.

[0029] In this invention, the product preferably further includes at least one of the following components: a thickener, a preservative, and a flavoring agent. The thickener preferably includes xanthan gum. The working concentration of the xanthan gum is preferably 0.1~0.2 g / 100 mL, more preferably 0.12~0.18 g / 100 mL, and even more preferably 0.15 g / 100 mL. The sweetener preferably includes at least one of the following: acesulfame potassium, xylitol, and steviol glycosides. The xanthan gum and the sweetener contribute to improving the sensory evaluation of the product. The preservative preferably includes potassium sorbate. The preservative helps inhibit microbial growth and does not destroy the hepatoprotective and alcohol-relieving active ingredients in the formulation.

[0030] This invention provides a method for preparing the product, comprising: mixing the components of the product to obtain the product. This invention does not impose any particular limitation on the mixing method of the product; conventional mixing methods in the art may be used.

[0031] This invention provides an application of the product in improving the activity of alcohol dehydrogenase.

[0032] This invention provides an application of the product in improving antioxidant properties.

[0033] In this invention, the cells preferably include liver cells, more preferably HepG2 cells. The enhancement of antioxidant capacity preferably includes increasing DPPH free radical scavenging rate.

[0034] This invention provides an application of the product in alleviating alcohol-induced cellular damage.

[0035] In this invention, the cells preferably include liver cells, more preferably HepG2 cells. The method for alleviating alcohol-induced cellular damage preferably includes adding the product to a cell culture medium. The cell culture medium preferably includes DMEM. After adding the product to the cell culture medium, the crude drug concentration of the product in the culture medium is 0.1 g / mL. This method for alleviating alcohol-induced cellular damage is more conducive to improving cell viability.

[0036] This invention provides an application of the product in the preparation of products for relieving hangovers and / or protecting the liver.

[0037] In this invention, the product is preferably a health food and / or a pharmaceutical.

[0038] Alcohol dehydrogenase is the core functional enzyme in the liver that metabolizes and breaks down alcohol. The embodiments of this invention demonstrate that the product prepared from this component can significantly enhance the activity of alcohol dehydrogenase, thereby accelerating the metabolism and breakdown of alcohol in the body. At the same time, the product can enhance the cell's own antioxidant capacity, thereby alleviating cell damage caused by alcohol. Therefore, it can be used to prepare a product with both hangover relief and liver protection effects.

[0039] To further illustrate the present invention, the solutions provided by the present invention will be described in detail below with reference to the accompanying drawings and embodiments, but these should not be construed as limiting the scope of protection of the present invention.

[0040] Preparation method of experimental materials (1) Preparation of hawthorn extract: ① Crushing: Wash fresh hawthorn in a container, remove the pits, and crush it with a pulping machine to obtain hawthorn pulp; ② Pre-cooking: Mix hawthorn pulp and water at a mass ratio of 1:10 and boil for 5 minutes; ③ Extraction: Extract in a water bath at 50℃ for 30 minutes to fully dissolve the components in hawthorn; ④ Filtration: Filter with four layers of gauze to remove the residue, and repeat the filtration twice to obtain hawthorn extract.

[0041] (2) Preparation of sea buckthorn extract: ① Juicing: Use a food-grade juicer to extract sea buckthorn juice. After juicing, add water with a volume of 2 times that of the sea buckthorn juice. ② Centrifugation: Centrifuge at 3000 r / min for 5 min and pour out the clear liquid at the top for later use. ③ Filtration: Filter through four layers of gauze, take the clear liquid in the middle, remove the coarse pulp and residue to obtain sea buckthorn extract.

[0042] (3) Preparation of Hovenia dulcis decoction: ① Pulverization: Hovenia dulcis is pulverized and passed through a 40-mesh sieve to obtain Hovenia dulcis powder; ② Soaking and decoction: Hovenia dulcis is soaked in water with 8 times its mass for 30 minutes, then decocted for 1 hour, and the filtrate is separated to obtain the decoction; the decoction is repeated twice, and the three decoctions are combined; ③ Filtration: the decoction is filtered through four layers of gauze and the filter residue is removed to obtain Hovenia dulcis decoction.

[0043] (4) Preparation of Gynostemma pentaphyllum decoction: ① Soaking and decoction: Soak Gynostemma pentaphyllum in 12 times the mass of water for 30 minutes, decoct for 1 hour, separate the filtrate to obtain the decoction; repeat the decoction twice and combine the three decoctions; ② Filtration: Filter the decoction with four layers of gauze and remove the filter residue to obtain Gynostemma pentaphyllum decoction.

[0044] (5) Preparation of decoction of Rhizoma Lysimachiae: ① Pulverization: Take dried Rhizoma Lysimachiae, pulverize and pass through a 40-mesh sieve; ② Soaking and decoction: Add purified water at a material-to-liquid ratio of 1:10 (g / mL), soak at room temperature for 30 min, decoct for 1 h, separate the filtrate, and obtain the decoction; repeat the decoction once more and combine the two decoctions (the effective components of Rhizoma Lysimachiae are relatively easy to dissolve, so there is no need to do it 3 times to avoid excessive bitterness); ③ Filtration: Filter the decoction with four layers of gauze to remove the dregs and obtain the decoction of Rhizoma Lysimachiae.

[0045] (6) Preparation of Akebia quinata (Akebia quinata) extract: ① Pulverization: Take dried Akebia quinata and wash it; ② Soaking and decoction: Add purified water at a material-to-liquid ratio of 1:12 (g / mL), soak for 30 min, decoct for 1 h, separate the filtrate, and obtain the decoction; repeat the decoction twice, and combine the three decoctions (the effective components of Akebia quinata dissolve slowly); ③ Filtration: Filter the decoction with four layers of gauze, remove the filter residue, and obtain Akebia quinata extract.

[0046] (7) Preparation of sweetener: Add 0.06g of acesulfame potassium, 6g of xylitol and 0.03g of steviol glycosides to 100mL of water to obtain sweetener.

[0047] Example 1 The following factors were selected for investigation: hawthorn extract, sea buckthorn extract, gynostemma pentaphyllum extract, Japanese raisin tree fruit extract, akebia quinata extract, rhubarb extract, sweetener, and xanthan gum content. Each factor had three levels, as detailed in Table 1. An orthogonal experimental design was used, with sensory evaluation as the comprehensive evaluation index to optimize the formula. The sensory evaluation criteria are shown in Table 2, and the orthogonal experimental groupings and sensory evaluation results are shown in Table 3.

[0048] Table 1. Experimental Factor Levels

[0049] Table 2 Sensory Evaluation Criteria

[0050] Table 3. Results of Orthogonal Experiment Grouping and Sensory Scores

[0051] Schemes 1 through 27 all involve preparing products by mixing each component with water, with a uniform total product volume of 50 mL. The following details the volume percentage of each component in the formulation and the concentration of xanthan gum within the formulation: Serial No. 1: Hawthorn extract 14%, sea buckthorn extract 8%, Gynostemma pentaphyllum extract 8%, Hovenia dulcis extract 16%, Akebia quinata extract 4%, Lysimachia christinae extract 1%, sweetener 12%, xanthan gum 0.05g / 50mL; Serial No. 2: Hawthorn extract 14%, sea buckthorn extract 8%, gynostemma pentaphyllum extract 8%, Japanese raisin tree extract 18%, akebia quinata extract 6%, lysimachia christinae extract 2%, sweetener 15%, xanthan gum 0.075g / 50mL; Serial No. 3: Hawthorn extract 14%, sea buckthorn extract 8%, gynostemma pentaphyllum extract 8%, Japanese raisin tree extract 20%, akebia quinata extract 8%, lysimachia christinae extract 3%, sweetener 18%, xanthan gum 0.1g / 50mL; Serial No. 4: Hawthorn extract 14%, sea buckthorn extract 10%, Gynostemma pentaphyllum extract 10%, Hovenia dulcis extract 16%, Akebia quinata extract 4%, Lysimachia christinae extract 2%, sweetener 15%, xanthan gum 0.1g / 50mL; Serial No. 5: Hawthorn extract 14%, sea buckthorn extract 10%, Gynostemma pentaphyllum extract 10%, Hovenia dulcis extract 18%, Akebia quinata extract 6%, Lysimachia christinae extract 3%, sweetener 18%, xanthan gum 0.05g / 50mL; Serial No. 6: Hawthorn extract 14%, sea buckthorn extract 10%, Gynostemma pentaphyllum extract 10%, Hovenia dulcis extract 20%, Akebia quinata extract 8%, Lysimachia christinae extract 1%, sweetener 12%, xanthan gum 0.075g / 50mL; Serial No. 7: Hawthorn extract 14%, sea buckthorn extract 12%, Gynostemma pentaphyllum extract 12%, Hovenia dulcis extract 16%, Akebia quinata extract 6%, Lysimachia christinae extract 3%, sweetener 12%, xanthan gum 0.1g / 50mL; Serial No. 8: Hawthorn extract 14%, sea buckthorn extract 12%, Gynostemma pentaphyllum extract 12%, Hovenia dulcis extract 18%, Akebia quinata extract 8%, Lysimachia christinae extract 1%, sweetener 15%, xanthan gum 0.05g / 50mL; Serial No. 9: Hawthorn extract 14%, sea buckthorn extract 12%, Gynostemma pentaphyllum extract 12%, Hovenia dulcis extract 20%, Akebia quinata extract 4%, Lysimachia christinae extract 2%, sweetener 18%, xanthan gum 0.075g / 50mL; Serial No. 10: Hawthorn extract 16%, sea buckthorn extract 8%, Gynostemma pentaphyllum extract 10%, Hovenia dulcis extract 16%, Akebia quinata extract 8%, Lysimachia christinae extract 2%, sweetener 18%, xanthan gum 0.075g / 50mL; Serial No. 11: Hawthorn extract 16%, sea buckthorn extract 8%, Gynostemma pentaphyllum extract 10%, Hovenia dulcis extract 18%, Akebia quinata extract 4%, Lysimachia christinae extract 3%, sweetener 12%, xanthan gum 0.1g / 50mL; Serial No. 12: Hawthorn extract 16%, sea buckthorn extract 8%, Gynostemma pentaphyllum extract 10%, Hovenia dulcis extract 20%, Akebia quinata extract 6%, Lysimachia christinae extract 1%, sweetener 15%, xanthan gum 0.05g / 50mL; Serial No. 13: Hawthorn extract 16%, sea buckthorn extract 10%, Gynostemma pentaphyllum extract 12%, Hovenia dulcis extract 16%, Akebia quinata extract 6%, Lysimachia christinae extract 1%, sweetener 18%, xanthan gum 0.1g / 50mL; Serial No. 14: Hawthorn extract 16%, sea buckthorn extract 10%, Gynostemma pentaphyllum extract 12%, Hovenia dulcis extract 18%, Akebia quinata extract 8%, Lysimachia christinae extract 2%, sweetener 15%, xanthan gum 0.05g / 50mL; Serial No. 15: Hawthorn extract 16%, sea buckthorn extract 10%, Gynostemma pentaphyllum extract 12%, Hovenia dulcis extract 20%, Akebia quinata extract 4%, Lysimachia christinae extract 3%, sweetener 12%, xanthan gum 0.075g / 50mL; Serial No. 16: Hawthorn extract 16%, sea buckthorn extract 12%, Gynostemma pentaphyllum extract 8%, Hovenia dulcis extract 16%, Akebia quinata extract 8%, Lysimachia christinae extract 3%, sweetener 15%, xanthan gum 0.05g / 50mL; Serial No. 17: Hawthorn extract 16%, sea buckthorn extract 12%, Gynostemma pentaphyllum extract 8%, Hovenia dulcis extract 18%, Akebia quinata extract 4%, Lysimachia christinae extract 1%, sweetener 18%, xanthan gum 0.075g / 50mL; Serial No. 18: Hawthorn extract 16%, sea buckthorn extract 12%, Gynostemma pentaphyllum extract 8%, Hovenia dulcis extract 20%, Akebia quinata extract 6%, Lysimachia christinae extract 2%, sweetener 12%, xanthan gum 0.1g / 50mL; Serial No. 19: Hawthorn extract 18%, sea buckthorn extract 8%, Gynostemma pentaphyllum extract 12%, Hovenia dulcis extract 16%, Akebia quinata extract 6%, Lysimachia christinae extract 3%, sweetener 15%, xanthan gum 0.075g / 50mL; Serial No. 20: Hawthorn extract 18%, sea buckthorn extract 8%, Gynostemma pentaphyllum extract 12%, Hovenia dulcis extract 18%, Akebia quinata extract 8%, Lysimachia christinae extract 1%, sweetener 18%, xanthan gum 0.1g / 50mL; Serial No. 21: Hawthorn extract 18%, sea buckthorn extract 8%, Gynostemma pentaphyllum extract 12%, Hovenia dulcis extract 20%, Akebia quinata extract 4%, Lysimachia christinae extract 2%, sweetener 12%, xanthan gum 0.05g / 50mL; Serial No. 22: Hawthorn extract 18%, sea buckthorn extract 10%, Gynostemma pentaphyllum extract 8%, Hovenia dulcis extract 16%, Akebia quinata extract 8%, Lysimachia christinae extract 1%, sweetener 12%, xanthan gum 0.075g / 50mL; Serial No. 23: Hawthorn extract 18%, sea buckthorn extract 10%, Gynostemma pentaphyllum extract 8%, Hovenia dulcis extract 18%, Akebia quinata extract 4%, Lysimachia christinae extract 2%, sweetener 15%, xanthan gum 0.1g / 50mL; Serial No. 24: Hawthorn extract 18%, sea buckthorn extract 10%, Gynostemma pentaphyllum extract 8%, Hovenia dulcis extract 20%, Akebia quinata extract 6%, Lysimachia christinae extract 3%, sweetener 18%, xanthan gum 0.05g / 50mL; Serial No. 25: Hawthorn extract 18%, sea buckthorn extract 12%, Gynostemma pentaphyllum extract 10%, Hovenia dulcis extract 16%, Akebia quinata extract 4%, Lysimachia christinae extract 3%, sweetener 18%, xanthan gum 0.075g / 50mL; Serial No. 26: Hawthorn extract 18%, sea buckthorn extract 12%, Gynostemma pentaphyllum extract 10%, Hovenia dulcis extract 18%, Akebia quinata extract 6%, Lysimachia christinae extract 1%, sweetener 12%, xanthan gum 0.1g / 50mL; Item No. 27: Hawthorn extract 18%, sea buckthorn extract 12%, Gynostemma pentaphyllum extract 10%, Hovenia dulcis extract 20%, Akebia quinata extract 8%, Lysimachia christinae extract 2%, sweetener 18%, xanthan gum 0.05g / 50mL.

[0052] Experimental data show that scheme number 17 received the highest sensory score.

[0053] Example 2: A product for relieving hangovers and protecting the liver Potassium sorbate was added to the product of Scheme 17 in Example 1 to obtain a product for relieving hangovers and protecting the liver, with a concentration of potassium sorbate of 0.4 g / kg.

[0054] The preparation method of the product for relieving hangovers and protecting the liver is as follows: Take 16mL of hawthorn extract, 12mL of sea buckthorn extract, 8mL of gynostemma pentaphyllum extract, 18mL of Japanese raisin tree extract, 4mL of akebia quinata extract, 1mL of lysimachia christinae extract, 18mL of sweetener, 0.15g of xanthan gum and 0.4g / kg of potassium sorbate, add water to make up to a total volume of 100mL, and the product for relieving hangovers and protecting the liver is obtained.

[0055] Comparative Example 1 This is a traditional Chinese medicine oral liquid with broad effects in relieving hangovers and protecting the liver. The product name is Haiwang Jinzun Brand Oyster Soybean Peptide Carnitine Oral Liquid, manufactured by Nanning Haiwang Health Biotechnology Co., Ltd. Its ingredients include oyster extract, soybean peptide powder, L-carnitine, taurine, fructose syrup, honey, soluble dietary fiber, β-cyclodextrin, vitamin C, zinc gluconate, vitamin B6, and peppermint flavoring.

[0056] Comparative Example 2 The preparation method of the product for relieving hangovers and protecting the liver is as follows: Take 18mL of Hovenia dulcis extract, 18mL of sweetener, 0.15g of xanthan gum and 0.4g / kg of potassium sorbate, add water to make up to a total volume of 100mL, and the product for relieving hangovers and protecting the liver is obtained.

[0057] Comparative Example 3 The preparation method of the product for relieving hangovers and protecting the liver is as follows: Take 12mL of sea buckthorn extract, 8mL of gynostemma pentaphyllum extract, 18mL of sweetener, 0.15g of xanthan gum and 0.4g / kg of potassium sorbate, add water to make up to a total volume of 100mL, and the product for relieving hangovers and protecting the liver is obtained.

[0058] Comparative Example 4 The preparation method of the product for relieving hangovers and protecting the liver is as follows: Take 16mL of hawthorn extract, 18mL of sweetener, 0.15g of xanthan gum and 0.4g / kg of potassium sorbate, add water to make up to a total volume of 100mL, and the product for relieving hangovers and protecting the liver is obtained.

[0059] Comparative Example 5 The preparation method of the hangover relief and liver protection product is as follows: Take 8mL of Gynostemma pentaphyllum extract, 18mL of sweetener, 0.15g of xanthan gum and 0.4g / kg of potassium sorbate, add water to make up to a total volume of 100mL, and obtain the hangover relief and liver protection product.

[0060] Comparative Example 6 The preparation method of the hangover relief and liver protection product is as follows: Take 4 mL of Akebia quinata extract, 0.15 g of xanthan gum and 0.4 g / kg of potassium sorbate, add water to make up to a total volume of 100 mL, and obtain the hangover relief and liver protection product.

[0061] Comparative Example 7 The preparation method of the product for relieving hangovers and protecting the liver is as follows: Take 1 mL of Tianjihuang extract, 18 mL of sweetener, 0.15 g of xanthan gum and 0.4 g / kg of potassium sorbate, add water to make up to a total volume of 100 mL, and the product for relieving hangovers and protecting the liver is obtained.

[0062] Comparative Example 8 The preparation method of the product for relieving hangovers and protecting the liver is as follows: Take 16mL of hawthorn extract, 12mL of sea buckthorn extract, 8mL of gynostemma pentaphyllum extract, 18mL of Japanese raisin tree extract, 18mL of sweetener, 0.15g of xanthan gum and 0.4g / kg of potassium sorbate, add water to make up to a total volume of 100mL, and the product for relieving hangovers and protecting the liver is obtained.

[0063] Comparative Example 9 The preparation method of the product for relieving hangovers and protecting the liver is as follows: Take 16mL of hawthorn extract, 12mL of sea buckthorn extract, 8mL of gynostemma pentaphyllum extract, 18mL of Japanese raisin tree extract, 4mL of akebia quinata extract, 18mL of sweetener, 0.15g of xanthan gum and 0.4g / kg of potassium sorbate, add water to make up to a total volume of 100mL, and the product for relieving hangovers and protecting the liver is obtained.

[0064] Test Example 1: In vitro alcohol detoxification activity determination Alcohol dehydrogenase (ADH) is abundant in the livers of humans and animals, as well as in plant and microbial cells. It is a zinc-containing metalloenzyme with broad substrate specificity. The alcohol dehydrogenase ethanol oxidation system is a major pathway for alcohol metabolism in the liver. The alcohol dehydrogenase activity was evaluated by measuring the activation rate of ADH. The in vitro ADH activation rates of the product in Example 2, the products in Comparative Examples 1-9, and distilled water were measured. Comparative Example 1 served as a positive control, and distilled water served as a negative control.

[0065] Zeroing the blank control group: Add 1.5 mL of sodium pyrophosphate solution (pH 8.8) to a test tube, and add 0.027 mol / L oxidized coenzyme I (NAD). + Add 1.0 mL of ethanol solution, then add 0.5 mL of 11.5% ethanol solution, add 0.1 mL of the test product, mix well, and incubate in a water bath at 25°C for 5 min. Immediately after the water bath, add 0.1 mL of 0.25 U / mL alcohol dehydrogenase solution to the test tube, mix well again, and immediately measure the absorbance at a wavelength of 340 nm using a spectrophotometer.

[0066] Measurements for each experimental group: The zeroing method was the same as that for the blank control group, except that distilled water was replaced with an 11.5% ethanol solution. Absorbance was measured at 340 nm using a spectrophotometer, with absorbance recorded every 10 seconds for 5 minutes.

[0067] Negative control: The testing method is the same as that of each experimental group, except that the test product is replaced with distilled water.

[0068] Calculate the increase in absorbance per minute. Based on the molar extinction coefficient of NADH generated at 340 nm being 6.2, calculate the activity unit of alcohol dehydrogenase, expressed as nanomoles of NADH generated per minute (nmol·min⁻¹). Calculate ADH activity using Formula I and activation rate using Formula II. ADH activity (U / mL) = ΔA / 10s × (total reaction volume / enzyme amount) × 10 6 / (6.2×10 3 ) Formula I; R(%) = (U1-U0) / U0×100 (Equation II) In the formula: R is the ADH activation rate, U1 is the ADH activity of each experimental group sample, and U0 is the ADH activity of the negative control group.

[0069] Experimental results ( Figure 1 As shown in Table 4, the alcohol dehydrogenase activation rate in Example 2 was 94.47%, while the activation rate in the positive control group (Comparative Example 1) was 89.31%. This indicates that there was no significant difference in in vitro alcohol detoxification activity between Example 2 and commercially available hangover remedies and liver-protecting health products. Furthermore, the alcohol dehydrogenase activation rate in Example 2 was significantly higher than that in other comparative examples, further demonstrating its effectiveness.

[0070] Table 4 Results of alcohol dehydrogenase activation rate

[0071] Test Example 2: In vitro antioxidant capacity determination The in vitro antioxidant activity of the products of Example 2 and Comparative Examples 1-9 was measured respectively, with Comparative Example 1 as a positive control.

[0072] The antioxidant activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) was determined by a free radical scavenging assay. First, 2.0 mg of DPPH reagent was weighed, dissolved in anhydrous ethanol, and diluted to a 50 mL brown volumetric flask. The solution was shaken well to obtain a 0.04 mg·mL⁻¹ DPPH stock solution.

[0073] Take 2 mL of sample solution into two stoppered test tubes. Add 2 mL of DPPH solution to one tube, mix well, and incubate at room temperature in the dark for 0.5 h. Measure the absorbance value Ai at 517 nm. Add 2 mL of anhydrous ethanol to the other tube, mix well, and incubate at room temperature in the dark for 0.5 h. Measure the absorbance value Aj at 517 nm. The anhydrous ethanol solution of DPPH (DPPH stock solution) serves as a blank control, with an absorbance value A0. Calculate the absorbance using Formula III.

[0074] K%=[1-(Ai-Aj) / A0]×100% Formula III; Experimental results ( Figure 2 As shown in Table 5, the DPPH free radical scavenging rate of the product in Example 2 was 86.33%, while the DPPH free radical scavenging rate of the commercially available traditional Chinese medicine health product for relieving hangovers and protecting the liver (Comparative Example 1) was 86.03%. The DPPH free radical scavenging rates of the other comparative examples were all lower than those of Example 2, indicating that the product in Example 2 has strong in vitro antioxidant activity.

[0075] Table 5. Results of DPPH free radical scavenging rate

[0076] Test Example 3: Cell Experiment HepG2 cells to be treated: HepG2 cells in logarithmic growth phase were adjusted to a concentration of 1×10⁻⁶ cells using DMEM medium (purchased from Shanghai Yuanye Biotechnology Co., Ltd.) 4 Cells / mL: 100 μL of cells were seeded into each well of a 96-well plate and cultured at 37°C for 24 h. After observing cell adhesion, the culture medium was aspirated to obtain the HepG2 cells to be treated.

[0077] Example 2 Drug-containing culture medium: Take the product obtained in Example 2, add it to DMEM basal culture medium and mix thoroughly to prepare a drug-containing culture medium with a crude drug concentration of 0.1-0.9 g / mL; the concentration is calculated as 0.1-0.9 g of raw medicinal material equivalent per milliliter of culture medium.

[0078] 1. Effects on HepG2 cell viability: Add 100 μL of the drug-containing culture medium from Example 2 to each well of HepG2 cells to be treated, and group them according to different drug concentrations, with 6 replicates per group.

[0079] In the experimental group, 100 μL of the drug-containing culture medium from Example 2 with a concentration of 0.1–0.9 g / mL was added to each well, while in the control group, 100 μL of DMEM culture medium was added.

[0080] After culturing at 37℃ for 24 h, the culture medium was discarded, and 90 μL of DMEM culture medium and 10 μL of CCK-8 reagent were added to each well. The cells were incubated at 37℃ for 3 h, and the absorbance value at a wavelength of 450 nm was measured. Based on this, the viability of HepG2 cells treated with different concentrations of drug-containing culture medium was calculated.

[0081] Experimental results show that ( Figure 3 As the drug concentration in the culture medium increased, cell viability gradually decreased. When the concentration exceeded 0.3 g / mL, the product exhibited significant toxicity to the cells.

[0082] 2. Establishment of an ethanol-induced alcoholic injury model in HepG2 cells: 100 μL of DMEM medium was added to each well of the HepG2 cells to be treated. Cells were grouped according to the volume percentage of ethanol in the added DMEM medium (0%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%), with 6 replicates per group. After 24 h of culture, the medium in each well was discarded, and 90 μL of DMEM medium and 10 μL of LCK-8 reagent were added to each well. The cells were incubated at 37°C for 3 h, and the absorbance at 450 nm was measured. The survival rate of HepG2 cells under different concentrations of ethanol stimulation was calculated based on this.

[0083] Experimental results show that ( Figure 4 As the percentage of ethanol increased, the cell survival rate decreased significantly. When the ethanol content was 2%, the cell survival rate was 45%. Therefore, the ethanol content used for cell modeling in subsequent experiments was 2%.

[0084] 3. Effects on alcohol-induced HepG2 cell viability: Model group: 100 μL of DMEM medium was added to each well of HepG2 cells to be treated and cultured for 24 h. The medium was then discarded and 2% ethanol solution was added and cultured for another 24 h. Control group: 100 μL of DMEM was added to each well of HepG2 cells to be treated and cultured for 24 h. The culture medium was then discarded, and fresh DMEM was added and cultured for another 24 h. Experimental group: HepG2 cells were added to each well at a concentration of 0. 1 After culturing in 100 μL of drug-containing medium with g / mL for 24 h, the medium was discarded, and 2% ethanol solution was added and culturing for another 24 h. The experimental groups were divided into groups according to the different drug-containing culture media. The drugs were the drug-containing culture media of Example 2 and the drug-containing culture media of Comparative Examples 1 to 9. The preparation method of the drug-containing culture media of Comparative Examples 2 to 9 was the same as that of the drug-containing culture media of Example 2. The preparation method of the drug-containing culture media of Comparative Example 1 was as follows: 100 mg of commercial liquid product was accurately weighed under sterile conditions, placed in a sterile container, and DMEM culture medium was added to make the final volume 1 mL. The mixture was thoroughly mixed to obtain the drug-containing culture media of Comparative Example 1 with a product mass concentration of 0.1 g / mL.

[0085] Each treatment was performed in 6 replicates. After 24 h of culture, the culture medium in each well was discarded, and 90 μL of DMEM medium and 10 μL of CCK-8 reagent were added to each well. The cells were incubated at 37 °C for 3 h, and the absorbance at 450 nm was measured. Based on this, the cell viability of different concentrations of drug-containing culture medium in the alcohol-induced HepG2 cell oxidative damage model was calculated.

[0086] Experimental results show that ( Figure 5(The figure shows 100% representing cell survival rate when the positive drug is administered). This product can significantly improve the alcohol-induced decrease in HepG2 cell viability, indicating that it has a good hepatoprotective effect. This confirms that the traditional Chinese medicine hangover-relieving and hepatoprotective beverage prepared in this invention has significant hangover-relieving activity.

[0087] Although the above embodiments have provided a detailed description of the present invention, they are only some embodiments of the present invention, and not all embodiments. Other embodiments can be obtained based on these embodiments without creative effort, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A product for relieving hangovers and protecting the liver, characterized in that, The components include the following components by volume: 8-12 parts of sea buckthorn extract, 16-20 parts of Japanese raisin tree decoction, 8-12 parts of gynostemma pentaphyllum decoction, 14-18 parts of hawthorn extract, 4-8 parts of akebia quinata decoction, and 1-3 parts of lysimachia christinae decoction.

2. The product according to claim 1, characterized in that, The seabuckthorn extract includes seabuckthorn juice; The hawthorn extract is obtained by boiling hawthorn pulp and water and then separating the liquid phase.

3. The product according to claim 1 or 2, characterized in that, The seabuckthorn extract is obtained by mixing seabuckthorn juice with water and then separating the liquid phase; the mass ratio of seabuckthorn juice to water is 1:(1~2). The hawthorn extract is obtained by mixing hawthorn pulp with water, boiling it, and then keeping it at a constant temperature. After keeping it at a constant temperature, the liquid phase is separated to obtain the hawthorn extract. The temperature for keeping it at a constant temperature is 45~55℃ and the time is 20~40min. The decoction of Japanese raisin tree fruit is obtained by boiling Japanese raisin tree fruit and water, separating the liquid phase to obtain the filtrate; the boiling is repeated twice, and the three filtrates are combined to obtain the decoction of Japanese raisin tree fruit; the mass ratio of Japanese raisin tree fruit to water is 1:(10~15). The Gynostemma pentaphyllum decoction is obtained by decocting Gynostemma pentaphyllum and water, separating the liquid phase to obtain the filtrate; the decoction is repeated twice, and the three filtrates are combined to obtain the Gynostemma pentaphyllum decoction; the mass ratio of Gynostemma pentaphyllum to water is 1:(10~15). The decoction of Akebia quinata is obtained by boiling Akebia quinata and water, separating the liquid phase to obtain the filtrate; the boiling is repeated twice, and the filtrates from the three times are combined to obtain the decoction of Akebia quinata; the mass ratio of Akebia quinata to water is 1:(10~15). The decoction of *Lysimachia christinae* is obtained by decocting *Lysimachia christinae* and water, separating the liquid phase, and then repeating the decoction once more, combining the two filtrates to obtain the decoction. The mass ratio of *Lysimachia christinae* to water is 1:(8~12).

4. The product according to claim 1, characterized in that, It also includes at least one of the following components: thickener, preservative and flavoring agent.

5. A method for preparing the product according to any one of claims 1 to 4, characterized in that, include: The components in the product are mixed to obtain the product.

6. The use of the product according to any one of claims 1 to 4 in improving the activity of alcohol dehydrogenase.

7. The use of the product according to any one of claims 1 to 4 in improving antioxidant properties.

8. The use of the product according to any one of claims 1 to 4 in alleviating alcohol-induced cellular damage.

9. The use of the product according to any one of claims 1 to 4 in the preparation of products for relieving hangovers and / or protecting the liver.

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