Traditional Chinese medicine and food formula for inhibiting proliferation of toxoplasma gondii and expelling toxoplasma gondii and application thereof

Abstract

The present application relates to a kind of traditional Chinese medicine medicine-food formula for inhibiting proliferation of toxoplasma and expelling toxoplasma, and preparation of the traditional Chinese medicine medicine-food formula includes the following weight of raw materials: 50-60 parts of Chinese plum, 20-30 parts of ginkgo nut, 20-30 parts of dandelion, 25-35 parts of polygonatum, 30-40 parts of white pachybrine, 5-15 parts of dried ginger, 25-35 parts of oyster, 30-40 parts of gordon euryale seed, 30-40 parts of medlar, 5-15 parts of radix astragali, 15-25 parts of licorice.The technical scheme of the present application can effectively inhibit the reproductive activity of toxoplasma, and gradually remove from human or animal body, and the drug has no toxic side effects.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine manufacturing, and in particular to a traditional Chinese medicine formula for inhibiting the proliferation of Toxoplasma gondii in humans and animals, its preparation method, and its application. Background Technology

[0002] Toxoplasma gondii can cause infection in humans and animals. It belongs to the smallest and simplest group of protozoan parasites. Cats and other felines are the definitive hosts of Toxoplasma gondii. It parasitizes the epithelial cells of the small intestine in these animals. After mating, the male and female gametophytes lay eggs, which are excreted in the feces and mix with the food of other mammals and birds, forming long-term cross-infection. It invades the cells of mammals and birds or parasitizes their nucleated cells, developing cysts in their tissues, causing: mechanical damage, nutrient depletion, toxin retention, space-occupying lesions, sensitization, and immune interference. Because the human immune system cannot eliminate Toxoplasma gondii, it can travel throughout the body via the blood and lymphatic system. It can invade any organ, including: brain, eyes, liver, myocardium, lungs, spleen, lymph nodes, kidneys, adrenal glands, pancreas, testes, skeletal muscle, bone marrow, pregnant women, and fetuses, making infected individuals susceptible to various diseases.

[0003] While most hospitals now perform toxoplasmosis antibody testing on pregnant women, current drug treatment generally uses spiramycin. This drug has low toxicity and is relatively safe for pregnant women; however, high doses can cause gastrointestinal reactions such as nausea, vomiting, and diarrhea. For other individuals showing positive results, sulfadiazine or pyrimethamine are commonly used. This treatment inhibits some extracellular Toxoplasma gondii tachyzoites but is almost ineffective against intracellular bradyzoites and Toxoplasma gondii cysts. Relapse or a return of positive antibody results is highly likely when the infected person's immunity declines. Furthermore, these drugs have significant side effects and are prone to inducing drug resistance. Summary of the Invention

[0004] The present invention aims to solve at least one of the technical problems existing in the prior art or related art.

[0005] Therefore, the purpose of this invention is to provide a traditional Chinese medicine formula for inhibiting the proliferation and eliminating Toxoplasma gondii, as well as its preparation method and application, which can inhibit the reproductive activity of Toxoplasma gondii and gradually eliminate it from the human or animal body by reducing its activity.

[0006] To achieve the above objectives, the first aspect of the present invention provides a traditional Chinese medicine formula for inhibiting and eliminating Toxoplasma gondii proliferation. The preparation of this traditional Chinese medicine formula includes the following raw materials by weight: 50-60 parts of dried plum, 20-30 parts of torreya nut, 20-30 parts of dandelion, 25-35 parts of polygonatum rhizome, 30-40 parts of poria cocos, 5-15 parts of dried ginger, 25-35 parts of oyster shell, 30-40 parts of fox nut, 30-40 parts of wolfberry, 5-15 parts of astragalus root, and 15-25 parts of licorice root.

[0007] In the above technical solution, preferably, the preparation of the traditional Chinese medicine food formula includes the following raw materials by weight: 55 parts of dried plum, 26 parts of torreya seed, 25 parts of dandelion, 32 parts of polygonatum, 38 parts of white poria, 13 parts of dried ginger, 33 parts of oyster, 34 parts of euryale seed, 37 parts of wolfberry, 13 parts of astragalus, and 25 parts of licorice.

[0008] The second aspect of this invention provides a method for preparing a traditional Chinese medicine formula for inhibiting and eliminating Toxoplasma gondii, comprising the following steps:

[0009] Take the ingredients according to the proportions, wash all the ingredients with purified water, and then let them air dry naturally;

[0010] The dried raw materials are mixed and pulverized into granules using a grinder. Water is then added and boiled to make a traditional Chinese medicine essence. The concentration ratio of the essence is 20:1.

[0011] The raw materials are fed into the SEF production equipment, CO2 is used as the extractant, distilled water is added, and supercritical extraction is carried out in an environment below 25°C to obtain the extract.

[0012] The anthelmintic drug is obtained by extracting water-soluble powder through spray drying.

[0013] In the above technical solution, preferably, the spray drying includes the following steps:

[0014] The extracted drug was sterilized by heating at 96℃ for 15 minutes;

[0015] The extract is then concentrated under vacuum to remove 75%-85% of the water content.

[0016] Under centrifugal force, the concentrated extract is sprayed into a mist through an atomizer in the drying chamber, thus drying the extract into granules.

[0017] The third aspect of the present invention provides a traditional Chinese medicine formula for inhibiting and eliminating Toxoplasma gondii proliferation in the preparation of animal feed that inhibits Toxoplasma gondii proliferation.

[0018] The fourth aspect of the present invention provides a traditional Chinese medicine preparation for inhibiting the proliferation of Toxoplasma gondii, characterized in that its active ingredient is the traditional Chinese medicine formula for inhibiting the proliferation and expelling Toxoplasma gondii provided in the first aspect of the present invention.

[0019] In the above technical solution, preferably, the dosage form of the traditional Chinese medicine preparation is granules, oral liquid, medicinal wine, or compressed candy.

[0020] Compared with existing technologies, the advantages of the traditional Chinese medicine formula for inhibiting and eliminating Toxoplasma gondii, its preparation method, and its application provided by this invention are as follows: The traditional Chinese medicine formula for inhibiting the proliferation of Toxoplasma gondii in humans and animals prepared by this invention has high drug purity, can effectively inhibit the reproductive activities of Toxoplasma gondii, and gradually eliminate it from the human or animal body, and the drug has no toxic side effects; it can improve cell quality, enhance human function, repair the immune system damaged by Toxoplasma gondii invasion, and ultimately get rid of diseases caused by Toxoplasma gondii infection through the self-regulation of the human or animal. Attached Figure Description

[0021] The above and / or additional aspects and advantages of the present invention will become apparent and readily understood from the description of the embodiments taken in conjunction with the following drawings, in which:

[0022] Figure 1 A production process flow chart of the extract of traditional Chinese medicine food formula involved in the embodiments of the present invention is shown;

[0023] Figure 2 A production process flow diagram of the oral liquid involved in the embodiments of the present invention is shown;

[0024] Figure 3 A flowchart illustrating the production process of compressed candy according to an embodiment of the present invention is shown. Detailed Implementation

[0025] To better understand the above-mentioned objectives, features, and advantages of the present invention, the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments. It should be noted that, unless otherwise specified, the embodiments and features described in these embodiments can be combined with each other.

[0026] Many specific details are set forth in the following description in order to provide a full understanding of the invention. However, the invention may also be practiced in other ways different from those described herein, and therefore the scope of protection of the invention is not limited to the specific embodiments disclosed below.

[0027] Example 1

[0028] This embodiment provides a traditional Chinese medicine formula for inhibiting and eliminating Toxoplasma gondii proliferation. The formula comprises the following ingredients by weight: 55 parts dried plum, 26 parts torreya seed, 25 parts dandelion, 32 parts polygonatum, 38 parts poria cocos, 13 parts dried ginger, 33 parts oyster shell, 34 parts euryale seed, 37 parts wolfberry, 13 parts astragalus, and 25 parts licorice.

[0029] like Figure 1 As shown, the traditional Chinese medicine food formula is a traditional Chinese medicine composition granule, and the specific preparation method is as follows:

[0030] Take the ingredients according to the proportions, wash all the ingredients with purified water, and then let them air dry naturally;

[0031] The dried raw materials are mixed and pulverized into granules using a grinder. Water is then added and boiled to make a traditional Chinese medicine essence. The concentration ratio of the essence is 20:1.

[0032] The raw materials are fed into the SEF production equipment, CO2 is used as the extractant, distilled water is added, and supercritical extraction is carried out in an environment below 25°C to obtain the extract.

[0033] The anthelmintic drug is obtained by extracting water-soluble powder through spray drying.

[0034] The spray drying includes the following steps:

[0035] The extracted drug was sterilized by heating at 96℃ for 15 minutes;

[0036] The extract is then concentrated under vacuum to remove 75%-85% of the water content.

[0037] Under centrifugal force, the concentrated extract is sprayed into a mist through an atomizer in the drying chamber, thus drying the extract into granules.

[0038] Example 2

[0039] This embodiment provides a traditional Chinese medicine formula for inhibiting and eliminating Toxoplasma gondii proliferation. The formula comprises the following ingredients by weight: 52 parts dried plum, 28 parts torreya seed, 22 parts dandelion, 27 parts polygonatum, 32 parts white poria, 7 parts dried ginger, 27 parts oyster shell, 32 parts euryale seed, 38 parts wolfberry, 7 parts astragalus, and 18 parts licorice.

[0040] like Figure 2 As shown, the traditional Chinese medicine formula is an oral liquid, and the specific preparation method is as follows:

[0041] Take the ingredients according to the proportions, wash all the ingredients with purified water, and then let them air dry naturally;

[0042] The dried raw materials are mixed and pulverized into granules using a grinder. Water is then added and boiled to make a traditional Chinese medicine essence. The concentration ratio of the essence is 20:1.

[0043] The raw materials are fed into the SEF production equipment, CO2 is used as the extractant, distilled water is added, and supercritical extraction is carried out in an environment below 25°C to obtain the extract.

[0044] The anthelmintic drug is obtained by extracting water-soluble powder through spray drying.

[0045] The spray drying includes the following steps:

[0046] The extracted drug was sterilized by heating at 96℃ for 15 minutes;

[0047] The extract is then concentrated under vacuum to remove 75%-85% of the water content.

[0048] Under centrifugal force, the concentrated extract is sprayed into a mist through an atomizer in the drying chamber, drying the extract into granules.

[0049] The traditional Chinese medicine oral liquid that inhibits the proliferation of Toxoplasma gondii is obtained by diluting 40-50 grams of powder granules with 450-500 grams of water. In this embodiment, the powder granules are diluted with 43 grams of water to 480 grams of water.

[0050] Example 3

[0051] This embodiment provides a traditional Chinese medicine formula for inhibiting and eliminating Toxoplasma gondii proliferation. The formula comprises the following ingredients by weight: 58 parts dried plum, 25 parts torreya seed, 21 parts dandelion, 26 parts polygonatum, 35 parts white poria, 15 parts dried ginger, 30 parts oyster shell, 30 parts euryale seed, 36 parts wolfberry, 14 parts astragalus, and 24 parts licorice.

[0052] like Figure 3 As shown, the traditional Chinese medicine food formula is a compressed candy, and the specific preparation method is as follows:

[0053] Take the ingredients according to the proportions, wash all the ingredients with purified water, and then let them air dry naturally;

[0054] The dried raw materials are mixed and pulverized into granules using a grinder. Water is then added and boiled to make a traditional Chinese medicine essence. The concentration ratio of the essence is 20:1.

[0055] The raw materials are fed into the SEF production equipment, CO2 is used as the extractant, distilled water is added, and supercritical extraction is carried out in an environment below 25°C to obtain the extract.

[0056] The anthelmintic drug is obtained by extracting water-soluble powder through spray drying.

[0057] The spray drying includes the following steps:

[0058] The extracted drug was sterilized by heating at 96℃ for 15 minutes;

[0059] The extract is then concentrated under vacuum to remove 75%-85% of the water content.

[0060] Under centrifugal force, the concentrated extract is sprayed into a mist through an atomizer in the drying chamber, drying the extract into granules.

[0061] The powder granules are mixed with oligomaltose as a binder, and after compression, they become tablets that inhibit the proliferation of Toxoplasma gondii.

[0062] Example 4

[0063] This embodiment provides a traditional Chinese medicine formula for inhibiting and eliminating Toxoplasma gondii proliferation. The formula comprises the following ingredients by weight: 51 parts dried plum, 21 parts torreya seed, 21 parts dandelion, 26 parts polygonatum, 31 parts poria cocos, 6 parts dried ginger, 26 parts oyster shell, 31 parts euryale seed, 31 parts wolfberry, 6 parts astragalus, and 16 parts licorice.

[0064] The aforementioned traditional Chinese medicine formula is applied to cat or dog food, and the specific preparation method is as follows:

[0065] Take the ingredients according to the proportions, wash all the ingredients with purified water, and then let them air dry naturally;

[0066] The dried raw materials are mixed and pulverized into granules using a grinder. Water is then added and boiled to make a traditional Chinese medicine essence. The concentration ratio of the essence is 20:1.

[0067] The raw materials are fed into the SEF production equipment, CO2 is used as the extractant, distilled water is added, and supercritical extraction is carried out in an environment below 25°C to obtain the extract.

[0068] The anthelmintic drug is obtained by extracting water-soluble powder through spray drying.

[0069] The spray drying includes the following steps:

[0070] The extracted drug was sterilized by heating at 96℃ for 15 minutes;

[0071] The extract is then concentrated under vacuum to remove 75%-85% of the water content.

[0072] Under centrifugal force, the concentrated extract is sprayed into a mist through an atomizer in the drying chamber, drying the extract into granules.

[0073] The powdered granules are mixed with maltodextrin as a binder to form pills with a diameter of 2 mm for later use.

[0074] Steam beef bone powder, duck meat, chicken breast, fish, corn, etc., until cooked, crush and mix them, then wrap them into 2 mm pills for feeding to pet cats or dogs, or other animals.

[0075] Example 5

[0076] This embodiment provides a traditional Chinese medicine formula for inhibiting and eliminating Toxoplasma gondii proliferation. The formula is prepared by the following ingredients by weight: 59 parts dried plum, 29 parts torreya seed, 29 parts dandelion, 34 parts polygonatum, 39 parts poria cocos, 14 parts dried ginger, 34 parts oyster shell, 39 parts euryale seed, 39 parts wolfberry, 14 parts astragalus, and 24 parts licorice.

[0077] The traditional Chinese medicine formula is a medicinal wine, and the specific preparation method is as follows:

[0078] Take the ingredients according to the proportions, wash all the ingredients with purified water, and then let them air dry naturally;

[0079] The dried raw materials are mixed and pulverized into granules using a grinder. Water is then added and boiled to make a traditional Chinese medicine essence. The concentration ratio of the essence is 20:1.

[0080] The raw materials are fed into the SEF production equipment, CO2 is used as the extractant, distilled water is added, and supercritical extraction is carried out in an environment below 25°C to obtain the extract.

[0081] The anthelmintic drug is obtained by extracting water-soluble powder through spray drying.

[0082] The spray drying includes the following steps:

[0083] The extracted drug was sterilized by heating at 96℃ for 15 minutes;

[0084] The extract is then concentrated under vacuum to remove 75%-85% of the water content.

[0085] Under centrifugal force, the concentrated extract is sprayed into a mist through an atomizer in the drying chamber, drying the extract into granules.

[0086] Mix 40-50 grams of medicinal powder with 450-500 ml of pure grain liquor, with an alcohol content of around 30%. Stir repeatedly for 3-5 days until sedimentation occurs, and use the clear liquor after one week.

[0087] In this embodiment, it is preferred to mix the powder with 42 grams of pure grain liquor at a ratio of 470 ml to 42 grams of medicinal powder.

[0088] Example 6

[0089] This embodiment provides a traditional Chinese medicine formula for inhibiting and eliminating Toxoplasma gondii proliferation. The formula is prepared by the following ingredients by weight: 59 parts dried plum, 29 parts torreya seed, 29 parts dandelion, 34 parts polygonatum, 39 parts poria cocos, 14 parts dried ginger, 34 parts oyster shell, 39 parts euryale seed, 39 parts wolfberry, 14 parts astragalus, and 24 parts licorice.

[0090] The traditional Chinese medicine food formula is an additive for animal feed, and the specific preparation method is as follows:

[0091] Take the ingredients according to the proportions, wash all the ingredients with purified water, and then let them air dry naturally;

[0092] The dried raw materials are mixed and pulverized into granules using a grinder. Water is then added and boiled to make a traditional Chinese medicine essence. The concentration ratio of the essence is 20:1.

[0093] The raw materials are fed into the SEF production equipment, CO2 is used as the extractant, distilled water is added, and supercritical extraction is carried out in an environment below 25°C to obtain the extract.

[0094] The anthelmintic drug is obtained by extracting water-soluble powder through spray drying.

[0095] The spray drying includes the following steps:

[0096] The extracted drug was sterilized by heating at 96℃ for 15 minutes;

[0097] The extract is then concentrated under vacuum to remove 75%-85% of the water content.

[0098] Under centrifugal force, the concentrated extract is sprayed into a mist through an atomizer in the drying chamber, drying the extract into granules.

[0099] 1 kg of the powder is added to 20-30 kg of animal feed and mixed. In this example, 1 kg of the powder is added to 21 kg of animal feed, such as pig feed.

[0100] The following section further illustrates the efficacy of this invention in terms of its clinical effects:

[0101] I. Animal inoculation test of the composition of the present invention

[0102] 1. Drugs: The present invention was used as the test drug in this experiment, and sulfadimidine sodium injection (batch number: National Drug Approval Number H23020552, Qiqihar Second Pharmaceutical Co., Ltd., specification: 2 ml contains 200 mg) was used as the Western medicine control.

[0103] 2. Toxoplasma gondii strain: The RH strain of Toxoplasma gondii was obtained from the College of Veterinary Medicine, Yangzhou University.

[0104] 3. Experimental animals: Kunming mice, clean grade, 188 mice, weighing 20-22 grams, half male and half female, purchased from Yangzhou University. Among them, 152 mice were used for drug treatment experiments, and the other 36 mice were used for median lethal dose determination experiments.

[0105] 4. Experimental Grouping: Mice were randomly divided into 4 groups, with 38 mice in each group. 20 mice were used to calculate the cure rate, 6 mice to count the number of parasites, and 12 mice to determine SOD activity. Group 1 was treated with the drug of this invention (prepared according to the method in Example 2) by gavage 4 hours after inoculation. The drug concentration was 1000 mg crude drug / mL, 0.5 mL per mouse, administered once daily twice for 5 days. Group 2 was treated with sulfadiazine sodium injection by gavage 4 hours after inoculation. The sulfadiazine was dissolved in physiological saline and prepared at a dosage of 125 mg crude drug / kg, 0.5 mL per mouse, administered once daily twice for 5 days. Group 3 received no medication but was treated with 0.5 mL of physiological saline by gavage 4 hours after inoculation, twice daily for 5 days as a positive control. Group 4 received no inoculation and no medication but was treated with 0.5 mL of physiological saline by gavage 5 days daily for 5 days as a negative control. The treatment results are shown in Table 1.

[0106] Table 1 Results of the artificial inoculation experiment with Toxoplasma gondii in mice

[0107]

[0108]

[0109] 5. Test methods:

[0110] (1) Resuscitation of the insect: The insect strain was removed from liquid nitrogen and quickly placed in warm water at 37°C for resuscitation. Mice were then injected intraperitoneally and passaged three times until the strain stabilized. The peritoneal fluid was then collected for later use.

[0111] (2) Median lethal dose (LD50) determination: The amount of parasites in the prepared peritoneal fluid was counted using a hemocytometer. Six groups were inoculated, with six mice in each group. The peritoneal fluid was inoculated with 5, 10, 15, 20, 25, and 30 parasites per mouse. The mice were observed for 20 days to determine mortality. The LD50 was 10 parasites per mouse. In the formal inoculation experiment, except for the negative control group, each mouse in the other groups was inoculated intraperitoneally with 20 Toxoplasma gondii tachyzoites RH strain per 0.3 ml. The mice in the negative control group were injected intraperitoneally with 0.3 ml of physiological saline per mouse.

[0112] (3) Administration time: Mice were treated with different drugs 4 hours after being inoculated with Toxoplasma gondii. During the experiment, the situation was observed and recorded every day, and the experiment ended after 20 days of inoculation.

[0113] (4) SOD activity assay: After inoculation with Toxoplasma gondii, on day 3 and day 6 of each group, blood was collected from the eyeballs of 6 mice and serum was prepared to measure serum SOD activity. The results are shown in Table 2.

[0114] (5) Parasite count: On the 7th day after inoculation with Toxoplasma gondii, peritoneal fluid was randomly collected from 6 mice in each group, examined under a microscope, and the number of parasites was counted. The results are shown in Table 3.

[0115] Table 2. Determination of serum SOD activity in mice of each group

[0116]

[0117]

[0118] In the early stages of the disease, mice exhibit a temporary increase in SOD (superoxide dismutase) activity to eliminate free radicals. Later, as the body consumes SOD to clear some free radicals and existing SOD is damaged by free radicals, SOD activity significantly decreases. Table 2 shows that the drug of this invention can increase and maintain SOD activity in the body, reducing the damage caused by free radicals.

[0119] Table 3. Average number of velocities in peritoneal fluid of mice in each group

[0120] Group <![CDATA[Average number of tachyzoites in peritoneal fluid / (×10⁴ cells·mL -1 )]]> Group 1 146.532±18.472 Group 2 143.424±21.344 Group 3 173.60±21.847 Group 4 106.426±18.940

[0121] The medicinal effects of each component in the traditional Chinese medicine formula described in this invention are as follows:

[0122] Ume plum: Sour and astringent in taste, neutral in nature. It enters the liver, spleen, lung, and large intestine meridians. It astringes the lungs, intestinal tract, promotes the production of body fluids, and expels roundworms. It is used for chronic cough due to lung deficiency, chronic diarrhea and dysentery, thirst due to deficiency heat, and vomiting and abdominal pain due to roundworm infestation.

[0123] Oysters: Salty and neutral in flavor, they protect the liver, enhance immunity, fight tumors, delay aging, and boost energy.

[0124] Dandelion: Bitter, sweet, neutral, slightly cold. Enters the liver and stomach meridians. Clears heat and detoxifies, promotes diuresis and dissipates nodules. Used for acute mastitis, lymphadenitis, scrofula, boils and carbuncles, acute conjunctivitis, fever due to colds, acute tonsillitis, acute bronchitis, gastritis, hepatitis, cholecystitis, and urinary tract infections.

[0125] Poria cocos: Sweet and bland in taste, neutral in nature. It enters the heart, lung, spleen, and kidney meridians. It promotes diuresis and eliminates dampness, strengthens the spleen, and calms the mind. It is used for edema with scanty urine, phlegm retention with dizziness and palpitations, spleen deficiency with poor appetite, restlessness, palpitations, and insomnia.

[0126] Polygonatum: Sweet, neutral. Enters the spleen, lung, and kidney meridians. Tonifies qi and nourishes yin, strengthens the spleen, moistens the lungs, and benefits the kidneys. Used to strengthen muscles and bones, replenish essence and qi, treat spleen and stomach weakness, pulmonary tuberculosis with hemoptysis, weakness of the lower limbs in children, thirst due to stomach heat, diabetes, improves eyesight, and treats parasites. It is a superior tonic for longevity, health, and brain health.

[0127] Torreya nuts: Sweet in taste, warm in nature. Enters the lung, stomach, and large intestine meridians. It has the effects of expelling parasites, eliminating food stagnation, and moisturizing dryness. It is used to treat abdominal pain due to intestinal parasites, infantile malnutrition, dry cough, constipation, hemorrhoids, etc.

[0128] Dried ginger: pungent and hot in nature. It enters the spleen, stomach, kidney, heart, and lung meridians. Dried ginger is pungent, hot, and warming. Entering the spleen and stomach meridians, it both dispels spleen and stomach deficiency-cold and assists spleen and stomach yang qi, making it an essential medicine for warming the middle and dispelling cold.

[0129] Euryale ferox: Sweet and astringent in taste, neutral in nature. It enters the spleen and kidney meridians. It tonifies the spleen and removes dampness, benefits the kidneys and consolidates essence. It has the advantages of being nourishing without being greasy and astringent without retaining dampness. It is mainly used to treat chronic diarrhea due to spleen deficiency and various symptoms of kidney deficiency and instability of the lower body.

[0130] Goji berries: They are sweet and neutral in nature, and enter the liver and kidney meridians. They are good at nourishing the liver and kidneys and improving eyesight, treating liver and kidney yin deficiency and decreased vision; they can also nourish lung yin and stop cough, treating yin deficiency cough.

[0131] Astragalus: Sweet in taste and warm in nature. It enters the lung and spleen meridians. Its functions include tonifying qi and strengthening the exterior, promoting diuresis and eliminating toxins, draining pus, and promoting wound healing and tissue regeneration.

[0132] Licorice: Sweet in taste and neutral in nature. It enters the heart, lung, spleen, and stomach meridians. It tonifies the spleen and replenishes qi, clears heat and detoxifies, resolves phlegm and relieves cough, alleviates spasms and pain, expels parasites and calms the mind, and harmonizes other herbs. It is used for spleen and stomach weakness, fatigue, palpitations and shortness of breath, abdominal and limb spasms and pain, carbuncles and boils, and to alleviate the toxicity and severity of other drugs.

[0133] Combined effects: Strengthens the body's resistance and clears toxins, invigorates the spleen and replenishes qi, restores the balance of the middle qi, eliminates dampness, clears heat and detoxifies, dries dampness and expels parasites.

[0134] It should be noted that the medicinal and edible drugs of this invention are not suitable for pregnant women and infants. When taking them, it is not advisable to drink strong tea or take other warming and tonifying Chinese medicines.

[0135] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A traditional Chinese medicine formula for inhibiting the proliferation of Toxoplasma gondii and eliminating Toxoplasma gondii, characterized in that, The preparation of this traditional Chinese medicine food formula includes the following raw materials by weight: 50-60 parts of dried plum, 20-30 parts of torreya nut, 20-30 parts of dandelion, 25-35 parts of polygonatum rhizome, 30-40 parts of white poria cocos, 5-15 parts of dried ginger, 25-35 parts of oyster shell, 30-40 parts of fox nut, 30-40 parts of wolfberry, 5-15 parts of astragalus root, and 15-25 parts of licorice root.

2. The traditional Chinese medicine formula for inhibiting Toxoplasma gondii proliferation and eliminating Toxoplasma gondii according to claim 1, characterized in that, The preparation of this traditional Chinese medicine food formula includes the following raw materials by weight: 55 parts dried plum, 26 parts torreya seed, 25 parts dandelion, 32 parts polygonatum, 38 parts white poria, 13 parts dried ginger, 33 parts oyster, 34 parts euryale seed, 37 parts wolfberry, 13 parts astragalus, and 25 parts licorice.

3. A method for preparing a traditional Chinese medicine formula for inhibiting Toxoplasma gondii proliferation and eliminating Toxoplasma gondii as described in claim 1 or 2, characterized in that, Includes the following steps: Take the ingredients according to the proportions, wash all the ingredients with purified water, and then let them air dry naturally; The dried raw materials are mixed and pulverized into granules using a grinder. Water is then added and boiled to make a traditional Chinese medicine essence. The concentration ratio of the essence is 20:

1. The raw materials are fed into the SEF production equipment, CO2 is used as the extractant, distilled water is added, and supercritical extraction is carried out in an environment below 25°C to obtain the extract. The anthelmintic drug is obtained by extracting water-soluble powder through spray drying.

4. The preparation method according to claim 3, characterized in that, The spray drying includes the following steps: The extracted drug was sterilized by heating at 96℃ for 15 minutes; The extract is then concentrated under vacuum to remove 75%-85% of the water content. Under centrifugal force, the concentrated extract is sprayed into a mist through an atomizer in the drying chamber, thus drying the extract into granules.

5. The application of the traditional Chinese medicine formula for inhibiting and eliminating Toxoplasma gondii as described in claim 1 or 2 in the preparation of animal feed that inhibits Toxoplasma gondii proliferation.

6. A traditional Chinese medicine preparation for inhibiting the proliferation of Toxoplasma gondii, characterized in that, Its active ingredient is the traditional Chinese medicine food formula for inhibiting the proliferation of Toxoplasma gondii and expelling Toxoplasma gondii as described in claim 1 or 2.

7. The traditional Chinese medicine preparation for inhibiting the proliferation of Toxoplasma gondii as described in claim 6, characterized in that, The dosage forms of the traditional Chinese medicine preparations are granules, oral liquids, medicinal wines, or compressed candies.

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