A pure natural huangqi ajiao peptide oral liquid and a preparation method thereof

Abstract

The application discloses a kind of pure natural huangqi peptan oral liquid and preparation method thereof, belong to health food technical field.The application is aimed at the problems of existing huangqi oral liquid, such as irritability, long processing cycle of traditional nine steaming nine sunning, difficulty in absorbing large molecules of gelatin, easy inactivation of high-temperature compounding, etc., by high-pressure steaming, calcium oxalate needle crystals are completely decomposed, and the irritation of numb tongue and sore throat is eliminated;High-pressure extraction greatly improves the dissolution rate of polysaccharide and saponin;Low-temperature compounding preserves the activity of small molecule gelatin peptide, and avoids sedimentation and stratification.The obtained oral liquid contains ≥5mg / mL of huangqi polysaccharide, has no irritation, good taste and good stability, and the gelatin peptide is a 500-2000Da small molecule, easy to absorb, suitable for industrial production.

Description

Technical Field

[0001] This invention relates to the field of health food technology, and in particular to a pure natural Polygonatum sibiricum donkey-hide gelatin peptide oral liquid and its preparation method. Background Technology

[0002] Polygonatum rhizome, the dried rhizome of a plant in the genus Polygonatum of the Liliaceae family, is a traditional Chinese medicine that is both food and medicine. It is believed to have effects such as tonifying qi and nourishing yin, strengthening the spleen and moistening the lungs, and benefiting the kidneys. Modern research shows that the polysaccharides, saponins, and flavonoids in Polygonatum rhizome have anti-fatigue and immune-enhancing effects. Donkey-hide gelatin, a solid gelatin made from the dried or fresh skin of the donkey (Equus equina) through boiling and concentration, is a traditional tonic with effects of nourishing blood and yin, moistening dryness, and stopping bleeding. Combining Polygonatum rhizome with donkey-hide gelatin is suitable for people with insufficient qi and blood, physical weakness, and low immunity.

[0003] However, existing technologies have the following technical defects in the preparation of Polygonatum and donkey-hide gelatin-related products:

[0004] The irritant effects of raw Polygonatum: Raw Polygonatum contains a large amount of calcium oxalate crystals, which can cause discomfort such as numbness of the tongue, irritation of the throat, and gastrointestinal irritation when taken directly, posing a safety risk.

[0005] Traditional processing techniques are inefficient: In order to remove the irritation of raw Polygonatum, the traditional processing technique of "nine steaming and nine drying" is used. However, this process takes dozens of days, has high labor costs, and the quality is unstable, making it difficult to achieve industrialized and large-scale production.

[0006] Low absorption and utilization of donkey-hide gelatin: Traditional donkey-hide gelatin is a large-molecule collagen protein with a large molecular weight, which is slow to be digested and absorbed by the human body. In addition, it is rich and greasy, which can easily cause problems such as bloating and indigestion, resulting in low bioavailability.

[0007] Low extraction efficiency of active ingredients: Conventional water extraction processes of Polygonatum (such as atmospheric pressure decoction) have low temperatures or short times, resulting in low dissolution rates of bound active ingredients such as Polygonatum polysaccharides and saponins, and weak product efficacy.

[0008] Compound formulation and stability issues: Mixing donkey-hide gelatin with Polygonatum extract at high temperatures can easily damage the structure of small molecule active peptides such as donkey-hide gelatin peptides, leading to their inactivation or precipitation. Simultaneously, the finished oral liquid is prone to precipitation and stratification, exhibiting poor stability.

[0009] Therefore, the market urgently needs a non-irritating, high-content active ingredient, easily absorbed small molecule, stable and industrially mass-producible Huangjing Ejiao peptide oral liquid and its preparation method. Summary of the Invention

[0010] The purpose of this invention is to address the shortcomings of existing technologies by proposing a pure natural Polygonatum odoratum and donkey-hide gelatin peptide oral liquid and its preparation method.

[0011] To achieve the above objectives, the present invention adopts the following technical solution: This invention first proposes a method for preparing a pure natural Polygonatum sibiricum and donkey-hide gelatin peptide oral liquid, comprising the following steps: Raw material pretreatment and high-pressure steaming modification: Raw Polygonatum is steamed under high pressure at a temperature of 110-130℃ and a pressure of 0.048-0.172MPa for 4-8 hours to obtain modified Polygonatum; Raw Polygonatum contains Polygonatum saponins and irritating mucilage; direct consumption can easily cause numbness in the mouth and tongue, and discomfort in the throat. High-temperature and high-pressure steaming can destroy the structure of the irritating substances, eliminate the numbness, and reduce toxicity, thus meeting the pretreatment requirements for raw materials that are both medicinal and edible.

[0012] The high-pressure, humid, and hot environment breaks down the cell walls and fiber structure of Polygonatum plants, allowing active ingredients such as Polygonatum polysaccharides, Polygonatum flavonoids, and saponins to be released from the dense tissue. At the same time, it moderately degrades large-molecule polysaccharides, reduces the degree of molecular polymerization, and improves the efficiency of subsequent water extraction.

[0013] Drying and shaping: The modified Polygonatum is dried until the moisture content is ≤10% to obtain dried Polygonatum; Reducing the moisture content of modified Polygonatum to below 10% serves two purposes: first, it inhibits microbial growth and prevents mold growth during raw material storage and extraction; second, it allows the Polygonatum tissue to dehydrate and shrink, and then rehydrate and swell upon contact with water, which is more conducive to the dissolution of active ingredients.

[0014] High-pressure enhanced water extraction: The dried Polygonatum is mixed with water at a material-to-liquid ratio of 1:5-1:10, and extracted under high pressure for 60-120 minutes at a temperature of 100-120℃ and a pressure of 0.1-0.2MPa to obtain an extract. The extract is then purified to obtain a water extract of Polygonatum. At normal pressure, water boils at 100℃. Under pressure, the water temperature can exceed the boiling point. The combination of high pressure and high temperature further breaks down the residual cell walls of plants. Water, as a polar solvent, efficiently extracts water-soluble polysaccharides, water-soluble saponins, amino acids and other effective substances from Polygonatum odoratum. Compared with extraction under normal pressure, the extraction rate is greatly improved.

[0015] If the water content is too low, the raw materials will not be sufficiently soaked, resulting in incomplete extraction; if the water content is too high, the costs of subsequent concentration and refining will increase. The right ratio balances extraction efficiency and production costs.

[0016] Low-temperature activation-locking compounding: The aqueous extract of Polygonatum odoratum is cooled to 40-50℃, and donkey-hide gelatin peptides are added and stirred to dissolve, thus obtaining a compounding solution; the amount of donkey-hide gelatin peptides added is 5%-10% of the total mass of the compounding solution; Donkey-hide gelatin peptides are small molecule active peptides and are heat-sensitive substances. At temperatures above 60℃, the peptide chains are easily denatured and hydrolyzed, resulting in loss of biological activity. Low-temperature environments can lock in the physiological activity of donkey-hide gelatin peptides. At the same time, this temperature range has good fluidity and allows for rapid and uniform dissolution when stirred.

[0017] Filling and sterilization: The compound solution is filled and sterilized to obtain Huangjing Ejiao Peptide Oral Solution.

[0018] Preferably, the high-pressure steaming time is 6 hours.

[0019] Too short a time will result in insufficient modification and irritating residues; too long a time will lead to the high-temperature decomposition of heat-sensitive active ingredients. 6 hours is the optimal time to balance detoxification, component retention and structural modification.

[0020] Preferably, in the drying and shaping step, the drying method is natural sun drying or low-temperature drying at 50-70℃.

[0021] Polygonatum polysaccharides are heat-sensitive substances; high-temperature drying will cause polysaccharides to oxidize, degrade, and be lost. Low-temperature / natural drying can maximize the preservation of the core active ingredients of Polygonatum, which is in line with the product positioning of pure natural products.

[0022] Preferably, in the high-pressure enhanced water extraction step, the refining process includes coarse filtration, centrifugation, and microfiltration performed sequentially.

[0023] Coarse filtration: Removes coarse fibers, residues, and other large, insoluble particles from Polygonatum odoratum; Centrifugation: Separates fine suspended impurities and turbid colloids, reducing the turbidity of the extract; Microfiltration: traps microorganisms, large molecular proteins, and tiny particles, improving the clarity and stability of oral liquids while reducing the interference of impurities on the activity of donkey-hide gelatin peptides.

[0024] The three-step refining process achieves clarification of the liquid and sterilization pretreatment, ensuring the taste and shelf life of the finished product.

[0025] Preferably, the molecular weight of the donkey-hide gelatin peptide is 500-2000 Da.

[0026] If the proportion is too low, the efficacy of donkey-hide gelatin peptides will not be significant; if the proportion is too high, it will easily cause the solution viscosity to increase and precipitation to occur, affecting the fluidity and stability of the oral liquid. This range takes into account both efficacy and physical properties of the product.

[0027] Large-molecule donkey-hide gelatin protein is difficult for the human gastrointestinal tract to absorb. Enzymatic hydrolysis yields small-molecule peptides of 500-2000 Da, which can be directly absorbed by the intestines without secondary digestion, and have a much higher bioavailability than ordinary donkey-hide gelatin. This molecular weight range is also the optimal range for active donkey-hide gelatin peptides, combining absorbability and physiological activity.

[0028] Preferably, the sterilization is performed using pasteurization, with sterilization conditions of 85°C for 15 minutes.

[0029] Instead of high-temperature and high-pressure sterilization (which easily destroys the activity of polysaccharides and peptides), pasteurization at low temperature is selected: 85℃ for 15 minutes can kill harmful microorganisms such as E. coli, mold, and yeast, meeting food hygiene standards; Low-temperature conditions do not damage the molecular structure of Polygonatum polysaccharides and donkey-hide gelatin peptides, and fully preserve their activity, making it the preferred sterilization method for heat-sensitive functional oral liquids.

[0030] This invention also proposes a pure natural Polygonatum odoratum and donkey-hide gelatin peptide oral liquid prepared by the aforementioned preparation method.

[0031] Preferably, the content of Polygonatum polysaccharide in the oral liquid is ≥5mg / mL.

[0032] Polygonatum polysaccharides are the core active ingredient of Polygonatum. This process employs a dual-enhanced extraction method combining high-pressure steaming and high-pressure water extraction to ensure that the polysaccharide content in the oral liquid is ≥5mg / mL. This content threshold is the effective concentration: below this concentration, the active ingredient dosage is insufficient and cannot exert its due effect; once the threshold is reached, physiological efficacy can be stably achieved, which is the core guarantee of the product's functionality.

[0033] Compared with the prior art, the beneficial effects of the present invention are: 1. In existing technologies, the calcium oxalate needle crystals contained in raw Polygonatum sibiricum are the direct cause of tongue numbness, throat irritation, and gastrointestinal irritation. While the traditional nine-steaming and nine-drying process can partially remove these crystals, it relies on multiple steaming and sun-drying processes, resulting in a mild but uneven effect, a long cycle, and unstable results. This invention employs a closed, high-pressure steaming process at 110-130℃ and 0.048-0.172MPa. Utilizing the strong physical effects of high temperature and pressure, the crystal structure of the calcium oxalate needle crystals fractures, disintegrates, and even transforms into amorphous forms, thereby eliminating the irritant at the material structure level in one step. Simultaneously, the high-pressure environment softens the cell wall fibrous tissue, allowing the medicinal properties of Polygonatum sibiricum to smoothly transform from "raw and cooling with irritation" to "sweet and nourishing," achieving a safe and stable taste and medicinal properties without repeated processing.

[0034] 2. Conventional atmospheric pressure water extraction can only dissolve a small amount of polysaccharides and saponins free in the intercellular spaces, while a large number of bound active ingredients are trapped by the dense cell walls and cannot be released. This invention uses water extraction at 100-120℃ and 0.1-0.2 MPa. On the one hand, the high pressure allows water molecules to penetrate more easily into the cells, and the pressure difference between the inside and outside of the cells causes irreversible rupture of the cell walls, forcibly releasing bound polysaccharides, saponins, and other components. On the other hand, the high-pressure environment increases the polarity and solubility of water, significantly enhancing the extraction efficiency of poorly soluble active substances. This mechanism increases the dissolution rate of Polygonatum polysaccharides, total saponins, and total phenols by 62% to more than 3.5 times compared to traditional processes, fundamentally ensuring the efficacy of the product.

[0035] 3. Donkey-hide gelatin peptides (500-2000 Da) are thermosensitive biomolecules. When temperatures exceed 55℃, their spatial structure is prone to unfolding, aggregation, and even degradation, leading to loss of activity and precipitation. Existing technologies often directly mix donkey-hide gelatin with high-temperature extracts, causing peptide chain denaturation and flocculation. This invention uniquely cools the Polygonatum extract to a constant temperature range of 40-50℃ before adding the donkey-hide gelatin peptides. This temperature ensures complete dissolution of the peptides while remaining far below their thermal denaturation critical point, thus fully preserving the natural conformation and biological activity of the small molecule peptides. Simultaneously, the low-temperature environment prevents thermally induced aggregation of the peptide chains. Combined with the three-stage purification process to remove impurities, the compound solution remains clear, non-stratified, and free of precipitation during long-term storage. This mechanism not only solves the shortcomings of traditional donkey-hide gelatin—its richness, irritation to the stomach, and poor absorption—but also meets the industrial requirements for long-term, stable, and effective products at room temperature. Detailed Implementation

[0036] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with existing known technologies. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.

[0037] Example 1: This embodiment provides a method for preparing pure natural Polygonatum sibiricum donkey-hide gelatin peptide oral liquid, the specific steps of which are as follows: Raw material pretreatment and high-pressure steaming modification: Fresh raw Polygonatum sibiricum free from mold and insect infestation is selected, cleaned and impurities removed, and then sliced ​​into pieces 0.5-1 cm thick. The sliced ​​Polygonatum sibiricum is placed in a high-pressure steamer and steamed at 120℃ and 0.1 MPa for 6 hours. This step, through the physical action of high temperature and high pressure, completely breaks down and decomposes calcium oxalate needle crystals, eliminating irritation; at the same time, it destroys the plant cell walls, softens the tissue, and transforms the medicinal properties from "cool and stimulating" to "sweet and nourishing," while pre-activating the bound active ingredients.

[0038] Drying and shaping: Remove the steamed Polygonatum slices and place them in a 60℃ oven for low-temperature drying until their moisture content drops below 10%. The drying process solidifies the modified tissue structure, prevents the backflow and degradation of active ingredients, and increases pore size to facilitate subsequent extraction solvent penetration.

[0039] High-pressure intensive water extraction: Dried Polygonatum sibiricum was mixed with water at a material-to-liquid ratio of 1:8 and placed in a high-pressure extraction tank. Extraction was performed at 110℃ and 0.15MPa for 90 minutes. The high-pressure environment completely ruptured the plant cell walls, promoting the release of bound Polygonatum sibiricum polysaccharides and saponins. After extraction, the extract was sequentially filtered through a 200-mesh screen, centrifuged at 5000rpm, and then microfiltered through a 0.45μm microporous membrane to obtain a clear and transparent Polygonatum sibiricum water extract.

[0040] Low-temperature activation-locking compounding: The aqueous extract of Polygonatum sibiricum is naturally cooled to 45℃. Under this constant temperature condition, donkey-hide gelatin peptide powder with a molecular weight of 500-2000 Da is slowly added while stirring. The amount of donkey-hide gelatin peptide added is 8% of the total mass of the system. Stir at a low speed until completely dissolved. This low-temperature compounding process completely preserves the spatial structure and biological activity of small molecule peptides, avoiding high-temperature denaturation, inactivation, and precipitation.

[0041] Filling and sterilization: The compounded liquid is immediately filled into glass bottles, sealed, and then pasteurized at 85℃ for 15 minutes to obtain the finished Huangjing Ejiao Peptide Oral Liquid.

[0042] Testing revealed that the oral liquid prepared in this embodiment contained 6.2 mg / mL of Polygonatum polysaccharide. The finished product was odorless, non-irritating, clear and transparent, and showed no sedimentation or stratification after being left at room temperature for 6 months.

[0043] Example 2: The basic steps of this embodiment are the same as those of Embodiment 1, the difference being the adjustment of process parameters.

[0044] High-pressure steaming: temperature is 110℃, pressure is 0.05MPa, and steaming time is 8 hours.

[0045] Drying: Sun-dry naturally until the moisture content is ≤10%.

[0046] High-pressure extraction: the material-to-liquid ratio is 1:5, the extraction temperature is 120℃, the pressure is 0.2MPa, and the extraction time is 60 minutes.

[0047] Low-temperature compounding: Cool to 40℃, and add 5% of the total mass of the system with donkey-hide gelatin peptide.

[0048] Testing showed that the oral liquid prepared in this embodiment contained 5.5 mg / mL of Polygonatum polysaccharide, and the finished product was non-irritating and had good stability.

[0049] Example 3: The basic steps of this embodiment are the same as those of Embodiment 1, the difference being the adjustment of process parameters.

[0050] High-pressure steaming: temperature 130℃, pressure 0.17MPa, steaming time 4 hours.

[0051] Drying: Dry at a low temperature of 70℃ until the moisture content is ≤10%.

[0052] High-pressure extraction: the material-to-liquid ratio is 1:10, the extraction temperature is 100℃, the pressure is 0.1MPa, and the extraction time is 120 minutes.

[0053] Low-temperature compounding: Cool to 50℃, and add 10% of the total mass of the system with donkey-hide gelatin peptide.

[0054] Testing showed that the oral liquid prepared in this embodiment contained 5.8 mg / mL of Polygonatum polysaccharide, and the finished product was non-irritating and had good stability.

[0055] Comparative Example 1: This comparative example uses the traditional "nine steaming and nine sun-drying" process to process Polygonatum sibiricum, and extracts it by boiling in water under normal pressure.

[0056] Processing of Polygonatum: The raw Polygonatum is repeatedly steamed and dried nine times according to traditional methods, taking about 30 days.

[0057] Extraction: Mix the processed Polygonatum with water at a ratio of 1:8, and decoct twice at 100℃ under normal pressure for 1.5 hours each time. Combine the extracts and filter.

[0058] Compound preparation: Crush the donkey-hide gelatin blocks directly and add them to the above hot extract (about 80°C) at a ratio of 8%, and stir to dissolve.

[0059] Filling and sterilization: Same as in Example 1.

[0060] Results: The finished oral liquid had a slightly numbing sensation in the mouth, the donkey-hide gelatin did not dissolve completely, and flocculent precipitate appeared after one week. Testing revealed that the content of Polygonatum polysaccharides was only 2.8 mg / mL.

[0061] Comparative Example 2: This comparative example uses high-pressure steaming, but does not involve low-temperature compounding of donkey-hide gelatin peptides.

[0062] The high-pressure steaming, drying, and high-pressure extraction steps of Polygonatum are the same as in Example 1.

[0063] Compound preparation: Add 8% of donkey-hide gelatin peptide to the 80℃ aqueous extract of Polygonatum odoratum and stir to dissolve.

[0064] Filling and sterilization: Same as in Example 1.

[0065] Results: A small amount of flocculent matter was observed during the compounding process. After being left at room temperature for one month, the finished oral liquid showed obvious precipitation and stratification.

[0066] Performance testing:

[0067] Data Analysis: Polysaccharides are one of the main active ingredients of Polygonatum, and their content directly reflects the extraction efficiency. The polysaccharide contents of Examples 1-3 were 6.2, 5.5, and 5.8 mg / mL, respectively, all exceeding the target value of 5 mg / mL. Comparative Example 1, using the traditional nine-steaming and nine-drying process followed by atmospheric pressure water extraction, had a polysaccharide content of only 2.8 mg / mL, less than 50% of the lowest value in the examples. This indicates that the high-pressure steaming + high-pressure enhanced water extraction process of this invention can effectively disrupt plant cell walls, promote the release of bound polysaccharides, and achieve significantly better extraction efficiency than traditional processes. In Example 2, due to the low material-to-liquid ratio (1:5), the polysaccharide content was slightly lower than in Example 1, but still much higher than in Comparative Example 1. Although Comparative Example 2 also used high-pressure extraction, achieving a polysaccharide content of 6.1 mg / mL, its subsequent stability was poor, indicating that high-pressure extraction itself makes a significant contribution to polysaccharide dissolution.

[0068] Raw Polygonatum rhizome contains calcium oxalate needle crystals, which can cause a numbing and throat-irritating sensation. Comparative Example 1, using a nine-steaming and nine-drying process, partially removed the irritation, but the finished product still had a slight numbing sensation, indicating that traditional processing methods may be uneven or incomplete. Examples 1-3 and Comparative Example 2 all used high-pressure steaming at 110-130℃ for 6 hours. The finished products were all non-irritating and had a sweet taste, proving that high-pressure steaming can break down and decompose the calcium oxalate needle crystals, eliminating the irritation. Simultaneously, high-pressure steaming transforms the medicinal properties of Polygonatum rhizome into a sweet and nourishing one, improving its palatability.

[0069] Stability is crucial for the commercialization of oral liquids. In Comparative Example 1, because the donkey-hide gelatin was directly pulverized and added to the hot extract, the large molecules of donkey-hide gelatin were not fully enzymatically hydrolyzed and incompletely dissolved, resulting in a large amount of flocculent precipitate after being left at room temperature. Although Comparative Example 2 used donkey-hide gelatin peptides, the compounding at a high temperature of 80℃ caused peptide chain denaturation and aggregation, resulting in obvious precipitation and stratification after one month. In contrast, Examples 1-3 used low-temperature compounding at 40-50℃, which fully preserved the spatial structure of small molecule peptides and avoided high-temperature denaturation; at the same time, the three-stage purification process (coarse filtration, centrifugation, and microfiltration) removed impurities, and the finished product remained clear and transparent without precipitation or stratification after being left at room temperature for 6 months, demonstrating excellent stability.

[0070] The activity of donkey-hide gelatin peptides is closely related to their molecular weight distribution; small molecule peptides of 500-2000 Da can be directly absorbed by the human body. In Examples 1-3, low-temperature compounding resulted in the proportion of 500-2000 Da peptides exceeding 90%. Comparative Example 1 used un-enzymatically hydrolyzed donkey-hide gelatin blocks, resulting in macromolecular aggregation and preventing the absorption of small molecules. Comparative Example 2, with its high-temperature compounding, caused peptide chain breakage or aggregation, broadened the molecular weight distribution, and reduced activity. This verifies the necessity of the "low-temperature activation lock" core step of this invention—temperatures exceeding 55°C will destroy the activity of donkey-hide gelatin peptides.

[0071] Based on the above data, the three-stage process of "high-pressure steaming to de-irritate, high-pressure extraction to enhance activity, and low-temperature compounding to preserve peptide activity" constructed in this invention forms a synergistic effect: high-pressure steaming eliminates irritation and pre-activates components, high-pressure extraction significantly increases polysaccharide yield, and low-temperature compounding preserves the activity of donkey-hide gelatin peptides and ensures long-term stability. Example 1 achieved optimal comprehensive performance under the parameter combination (steaming at 120℃ for 6 hours, drying at 60℃, extraction at 110℃ / 0.15MPa, and compounding with 8% donkey-hide gelatin peptides at 45℃). Comparative Example 1, due to its outdated processing and extraction methods, had poor polysaccharide content and stability; Comparative Example 2, although having a high polysaccharide content, failed to maintain stability due to high-temperature compounding, further demonstrating the indivisibility of the overall process of this invention.

[0072] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A method for preparing a pure natural Polygonatum odoratum and donkey-hide gelatin peptide oral liquid, characterized in that, Includes the following steps: Raw material pretreatment and high-pressure steaming modification: Raw Polygonatum is steamed under high pressure at a temperature of 110-130℃ and a pressure of 0.048-0.172MPa for 4-8 hours to obtain modified Polygonatum; Drying and shaping: The modified Polygonatum is dried until the moisture content is ≤10% to obtain dried Polygonatum; High-pressure enhanced water extraction: The dried Polygonatum odoratum is mixed with water at a material-to-liquid ratio of 1:5-1:10, and extracted under high pressure for 60-120 minutes at a temperature of 100-120℃ and a pressure of 0.1-0.2MPa to obtain an extract. The extract is then purified to obtain a water extract of Polygonatum odoratum. Low-temperature activation-locking compounding: The aqueous extract of Polygonatum odoratum is cooled to 40-50℃, and donkey-hide gelatin peptides are added and stirred to dissolve, thus obtaining a compounding solution; the amount of donkey-hide gelatin peptides added is 5%-10% of the total mass of the compounding solution; Filling and sterilization: The compound solution is filled and sterilized to obtain Huangjing Ejiao Peptide Oral Solution.

2. The preparation method according to claim 1, characterized in that, The high-pressure steaming time is 6 hours.

3. The preparation method according to claim 1, characterized in that, In the drying and shaping step, the drying method is natural sun drying or low-temperature drying at 50-70℃.

4. The preparation method according to claim 1, characterized in that, In the high-pressure enhanced water extraction step, the refining process includes coarse filtration, centrifugation, and microfiltration performed sequentially.

5. The preparation method according to claim 1, characterized in that, The molecular weight of the donkey-hide gelatin peptide is 500-2000 Da.

6. The preparation method according to claim 1, characterized in that, The sterilization process employs pasteurization, with sterilization conditions of 85°C for 15 minutes.

7. A pure natural Polygonatum odoratum and donkey-hide gelatin peptide oral liquid, characterized in that, It is prepared by the preparation method according to any one of claims 1-6.

8. The pure natural Polygonatum odoratum and donkey-hide gelatin peptide oral liquid according to claim 7, characterized in that, The oral liquid contains ≥5 mg / mL of Polygonatum polysaccharide.

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