Culture medium sterilizer with dynamic mixing function

Abstract

The application provides a culture medium sterilizer with dynamic mixing function, which comprises a sterilizer cavity assembly, a rotating frame assembly, a fixed disc assembly and a driving motor assembly. The sterilizer cavity assembly comprises a sterilizer cavity, an inner sterilization chamber is formed in the sterilizer cavity; the rotating frame assembly is arranged in the sterilizer cavity and comprises a rotating frame, a fixed support rod and a planetary gear assembly, a gear ring of the planetary gear assembly is fixedly arranged relative to the sterilizer cavity, the fixed support rod is rotatably arranged in the rotating frame and coaxially fixedly connected with a planetary gear of the planetary gear assembly; the fixed disc assembly is fixedly installed on the fixed support rod and is circumferentially provided with a container fixing clamping groove for fixing a container; the driving motor assembly is in transmission connection with the rotating frame. The application realizes compound motion by planetary gear train transmission, so that the container revolves with the rotating frame and simultaneously rotates around its own axis, the turbulence effect of the culture medium is enhanced, the component precipitation is avoided, the heating uniformity is improved, and the sterilization is ensured to be complete.

Description

Technical Field

[0001] This invention relates to the field of microbial culture technology, and more specifically, to a culture medium sterilizer with dynamic mixing function. Background Technology

[0002] In biotechnology industrial production, the large-scale preparation of sterile solid, semi-solid, and liquid culture media is a fundamental step in culturing industrial microorganisms. The aseptic preparation of these media almost always requires the use of a sterilizer. However, traditional sterilizers typically operate with the internal containers in a static state, lacking rotation or stirring capabilities. This structural deficiency leads to two problems: first, the culture medium within the containers cannot be dynamically mixed, easily resulting in component precipitation or system inhomogeneity, affecting the quality of the culture medium; second, uneven heating of the culture medium within the containers in a static state may lead to incomplete sterilization due to localized insufficient temperatures, thus affecting the subsequent inoculation and cultivation of industrial microorganisms.

[0003] Patent document CN121891574A discloses a double-door motorized pulsed vacuum sterilizer that uses pressurized saturated steam to sterilize items, primarily for sterilizing medical instruments. However, it cannot achieve real-time sterilization of solid, semi-solid, and liquid culture media, nor can it mix the culture media within the container in real time. Patent document CN121971664A discloses a water bath sterilizer for narrow-necked glass bottles, using a stepped rack to ensure each layer of bottles receives efficient hot water spray, thus improving sterilization efficiency. However, this device also cannot mix the culture media within the container in real time or ensure uniform heating, nor can it add heat-sensitive nutrients to test tube or petri dish culture media after sterilization and cooling to achieve continuous preparation of semi-solid or solid culture media such as test tubes or petri dishes. The aforementioned devices have relatively limited functionality and cannot systematically solve problems such as insufficient mixing, uneven heating, and cumbersome preparation operations during culture media sterilization.

[0004] Therefore, there is an urgent need in the field for a sterilizer that can simultaneously achieve dynamic mixing of culture medium, uniform heating, and post-sterilization shaping operations, in order to systematically solve the above-mentioned technical problems. Summary of the Invention

[0005] In view of the deficiencies in the prior art, the purpose of this invention is to provide a culture medium sterilizer with dynamic mixing function.

[0006] The culture medium sterilizer with dynamic mixing function provided by the present invention includes: A sterilizer cavity assembly, including a sterilizer cavity, the interior of which forms a sterilization chamber; A rotating frame assembly is disposed inside the sterilizer cavity. The rotating frame assembly includes a rotating frame, a fixed support rod, and a planetary gear assembly. The gear ring of the planetary gear assembly is fixedly disposed relative to the sterilizer cavity, and the fixed support rod is rotatably disposed in the rotating frame and is coaxially and fixedly connected to the planetary gear of the planetary gear assembly. The fixed plate assembly is fixedly installed on the fixed support rod, and the fixed plate assembly is provided with a container fixing slot for fixing the vessel along the circumferential direction. The drive motor assembly is connected to the rotating frame via a transmission.

[0007] Preferably, the rotating frame includes a rotating shaft and a rotating support disk coaxially fixedly mounted on the outside of the rotating shaft; The rotating support disk has a second slot corresponding to the planetary gear along its circumferential direction, and the fixed support rod is rotatably inserted into the second slot; the top and bottom of the sterilizer cavity are respectively provided with a rotating frame top slot and a rotating frame bottom slot; The top end of the rotating shaft is rotatably installed in the top slot of the rotating frame, and the bottom end of the rotating shaft is rotatably installed in the bottom slot of the rotating frame; a toothed ring fixing groove is provided on the inner wall of the sterilizer cavity, and the toothed ring is fixedly installed in the toothed ring fixing groove.

[0008] Preferably, the sterilizer cavity assembly further includes a door disposed on the side of the sterilizer cavity; One side of the door is hinged to the sterilizer cavity, and the other side of the door is provided with a door bolt groove. The sterilizer cavity is provided with a door bolt corresponding to the door bolt groove, and the door bolt is provided with a movable fastening handwheel.

[0009] Preferably, the outer wall of the sterilizer cavity is provided with a jacket for the flow of heat exchange medium; The sterilizer cavity is provided with an industrial steam inlet and an industrial steam outlet communicating with the jacket, as well as a cooling water inlet and a cooling water outlet communicating with the jacket. The sterilizer cavity is also provided with a pure steam inlet and a pure steam outlet communicating with the sterilization chamber, as well as a compressed air inlet and a compressed air outlet communicating with the sterilization chamber.

[0010] Preferably, the sterilizer cavity is provided with a first jacket temperature sensor and a second jacket temperature sensor for detecting jacket temperature, a first inner chamber temperature sensor and a second inner chamber temperature sensor for detecting sterilization chamber temperature, a first jacket pressure sensor and a second jacket pressure sensor for detecting jacket pressure, and a first inner chamber pressure sensor and a second inner chamber pressure sensor for detecting sterilization chamber pressure.

[0011] Preferably, the drive motor assembly further includes a drive motor mounting bracket and a sterilizer cavity support; The drive motor is fixedly installed inside the drive motor mounting bracket by drive motor fixing bolts, and the drive motor mounting bracket is located inside the sterilizer cavity support; The sterilizer cavity support is fixedly connected to the bottom of the sterilizer cavity by sterilizer cavity fixing bolts, and the bottom end of the rotating frame passes through the bottom of the sterilizer cavity and is connected to the drive shaft of the drive motor.

[0012] Preferably, multiple layers of rotating support discs are installed axially spaced on the outside of the rotating shaft, and each rotating support disc forms a rotating disc mounting space; Each rotating support disk has multiple fixed disk assemblies arranged circumferentially, and each fixed support rod has multiple fixed disk assemblies arranged axially at intervals.

[0013] Preferably, it also includes a mobile platform assembly; The mobile platform assembly includes a platform, wheels disposed at the bottom of the platform, brakes disposed on the wheels, and a handle disposed at one end of the platform. The sterilizer cavity assembly is fixedly installed on the platform, and the drive motor assembly is installed between the sterilizer cavity assembly and the platform.

[0014] Preferably, the fixed support rod is provided with a first mounting position and a second mounting position spaced apart along the axial direction, wherein the second mounting position is higher than the first mounting position; The fixing plate assembly is fixedly sleeved on the first mounting position of the fixing support rod for fixing the barrel-type container; A rotating sleeve assembly is fixedly sleeved on the second mounting position. A rotating connecting rod is rotatably provided on the rotating sleeve assembly, and the rotating sleeve assembly is provided with a transmission engagement mechanism for driving the rotating connecting rod to rotate around its own axis. An inclined plane fixing component is fixedly installed at the end of the rotating connecting rod, and the inclined plane fixing component is used to fix the test tube at an angle.

[0015] Preferably, the fixed support rod is provided with a first mounting position and a second mounting position spaced apart along the axial direction, wherein the second mounting position is higher than the first mounting position; The fixed plate assembly is fixedly sleeved on the first mounting position of the fixed support rod and is used to fix the bottom of the plate. A rotating sleeve assembly is fixedly sleeved on the second mounting position. A rotating connecting rod is rotatably provided on the rotating sleeve assembly, and the rotating sleeve assembly is provided with a first transmission engagement mechanism for driving the rotating connecting rod to rotate around its own axis. A chuck assembly is fixedly mounted on the outer side of the rotating sleeve assembly. The chuck assembly includes a radially openable and closable jaw assembly and a second transmission engagement mechanism that is driven by the rotating connecting rod. The second transmission engagement mechanism is used to drive the jaw assembly to open and close under the rotation drive of the rotating connecting rod to clamp the lid of the plate.

[0016] Compared with the prior art, the present invention has the following beneficial effects: 1. This invention utilizes the cooperation of a rotating frame assembly, a planetary gear assembly, and a fixed disk assembly. When the rotating frame is driven by a motor, the fixed disk assembly rotates synchronously around its own axis while revolving with the rotating frame. This allows the vessels to simultaneously achieve a combined motion of revolution and rotation, significantly enhancing the turbulence effect of the culture medium. This solves the problem of traditional sterilizers being unable to dynamically mix the medium, avoids component precipitation and system inhomogeneity, and also helps improve heating uniformity, preventing incomplete sterilization in certain areas.

[0017] 2. This invention, by staggering the fixed plate assembly and the rotating sleeve assembly on the fixed support rod, ensures that the barrel-shaped container and the inclined fixed test tube do not interfere with each other in the vertical direction, achieving simultaneous compatible support for both types of containers, avoiding batch sterilization, and improving production efficiency. The transmission and engagement mechanism of the rotating sleeve assembly drives the rotating connecting rod to rotate the inclined plane fixing assembly to the target angle and then locks it, so that the culture medium in the test tube directly solidifies into a uniform angle after sterilization, realizing the integrated operation of sterilization and inclined plane forming, and solving the problems of low efficiency and inconsistent angles in large-scale inclined plane preparation.

[0018] 3. This invention uses a fixed support rod with staggered fixed plate components and rotating sleeve components to separate the bottom and lid of the petri dish vertically, eliminating sterilization dead zones caused by gaps in the mating surfaces when the dish is closed. The lid is held in the upper space by a chuck assembly, avoiding additional occupation of table space. The claw assembly opens and closes radially under the drive of the rotating connecting rod, realizing the synchronous clamping and release of a batch of lids. After sterilization and cooling, the lid can be directly grabbed inside the equipment and heat-sensitive nutrients can be dripped onto the bottom of the dish. Sterilization and the addition of heat-sensitive nutrients are completed continuously in the same equipment without the need to transfer the petri dishes. This achieves integrated processing of petri dish culture medium sterilization and shaping, greatly reducing the risk of contamination of sterilized culture medium.

[0019] 4. This invention achieves full-process control of the sterilization chamber's heating, sterilization, cooling, purging, pressure holding, and drying processes by installing a jacket on the sterilizer cavity and providing inlet and outlet pipelines for industrial steam, cooling water, pure steam, and compressed air, along with multiple sets of temperature and pressure sensing elements, thus ensuring the stability and repeatability of the sterilization process.

[0020] 5. By setting up a mobile platform component, the present invention allows the sterilizer to be flexibly moved to different workstations without fixed installation, thereby improving the equipment's flexibility and site adaptability. Attached Figure Description

[0021] Other features, objects, and advantages of the present invention will become more apparent from the following detailed description of non-limiting embodiments with reference to the accompanying drawings: Figure 1 This is a schematic diagram of the overall structure of the present invention; Figure 2 This is a schematic diagram of the sterilizer cavity assembly from one angle in this invention; Figure 3 This is a schematic diagram of the sterilizer cavity assembly from another angle in this invention; Figure 4 This is a schematic diagram of the assembly of the mobile platform assembly and the drive motor assembly in this invention; Figure 5 This is a schematic diagram of the structure of the mobile platform assembly in this invention; Figure 6 This is a schematic diagram of the rotating frame assembly in this invention; Figure 7 This is a structural schematic diagram of the rotating frame assembly in this invention from a bottom-view angle; Figure 8 This is a structural schematic diagram of the rotating frame assembly in this invention from a frontal view. Figure 9 This is a schematic diagram of the rotating frame in this invention; Figure 10 This is a schematic diagram of the assembly of the rotating frame and the fixed support rod in this invention; Figure 11 This is a schematic diagram of the fixed disk assembly in this invention; Figure 12 This is a partially enlarged schematic diagram of the fixed support rod in this invention; Figure 13 This is an assembly diagram of the fixed support rod in this invention; Figure 14 This is an assembly diagram of the fixed support rod, the fixed disk assembly, and the inclined plane fixing assembly in this invention; Figure 15 This is a top view assembly diagram of the fixed support rod, fixed disk assembly, and inclined plane fixing assembly in this invention; Figure 16 This is a schematic diagram of the assembly of the inclined plane fixing component and the rotating sleeve component in this invention; Figure 17 This is a schematic diagram of the rotating sleeve assembly in this invention; Figure 18 This is a schematic diagram of the inclined plane fixing component in this invention; Figure 19 This is an assembly diagram of the fixed support rod, the fixed disk assembly, and the flat plate fixing assembly in this invention; Figure 20 This is a top view assembly diagram of the fixed support rod, fixed plate assembly, and flat plate fixing assembly in this invention; Figure 21 This is a schematic diagram of the structure of the plate fixing component in this invention; Figure 22 This is a schematic diagram of the chuck assembly in this invention; Figure 23 This is an exploded view of the chuck assembly in this invention; Figure 24 This is a schematic diagram of the internal components of the chuck assembly in this invention; Figure 25 This is a schematic diagram of the structure of the present invention when used in barrel-shaped containers and test tubes; Figure 26 This is a schematic diagram of the structure of the present invention when used in a flat dish.

[0022] The diagram shows: Detailed Implementation

[0023] The present invention will now be described in detail with reference to specific embodiments. These embodiments will help those skilled in the art to further understand the present invention, but do not limit the invention in any way. It should be noted that those skilled in the art can make several changes and improvements without departing from the concept of the present invention. These all fall within the protection scope of the present invention.

[0024] This invention discloses a culture medium sterilizer with dynamic mixing function. Through the cooperation of a rotating frame assembly, a planetary gear assembly, and a fixed disk assembly, when the rotating frame is driven by a drive motor, the fixed disk assembly, while revolving with the rotating frame, simultaneously rotates around its own axis under the meshing transmission of a fixed gear ring and planetary gears. This allows the vessels to simultaneously experience a combined motion of revolution and rotation, significantly enhancing the turbulence effect of the culture medium. This solves the problem of traditional sterilizers being unable to dynamically mix the medium, avoiding component precipitation and system inhomogeneity. It also helps improve heating uniformity and prevents incomplete sterilization in certain areas. The following specific embodiments illustrate the solution of this invention.

[0025] Example 1 like Figures 1 to 5As shown, this embodiment provides a culture medium sterilizer with dynamic mixing function, including a sterilizer cavity assembly 300, a rotating frame assembly 400, a fixed plate assembly 500, and a drive motor assembly 200.

[0026] The sterilizer chamber assembly 300 includes a sterilizer chamber 305, the interior of which forms a sterilization chamber for accommodating culture media vessels to be sterilized. A rotating frame assembly 400 is disposed inside the sterilizer chamber 305, and includes a rotating frame 406, a fixed support rod 416, and a planetary gear assembly. The gear ring 403 of the planetary gear assembly is fixedly disposed relative to the sterilizer chamber 305. The fixed support rod 416 is rotatably disposed within the rotating frame 406 and coaxially fixedly connected to the planetary gears 404 of the planetary gear assembly. A fixed plate assembly 500 is fixedly mounted on the fixed support rod 416, and the fixed plate assembly 500 has a container fixing slot 502 along its circumferential direction for fixing the vessels. A drive motor assembly 200 is connected to the rotating frame 406 for transmission, providing power to the entire sterilizer.

[0027] When using the sterilizer in this embodiment, the following operating procedure should be followed: With the drive motor 201 stationary, loosen the locking handwheel 304, remove the door bolt 303 from the door bolt slot 302, and open the door 301. Carefully place the container containing the culture medium to be sterilized (actually sterilized) into the container fixing slot 502 of the fixing tray assembly 500, close the door 301, place the door bolt 303 into the door bolt slot 302, and tighten the locking handwheel 304 to seal and lock the door 301 with the sterilizer cavity 305.

[0028] The sterilizer chamber 305 has a jacket on its outer wall for the flow of heat exchange medium. The sterilizer chamber 305 has an industrial steam inlet 312 and an industrial steam outlet 309 communicating with the jacket, as well as a cooling water outlet 310 and a cooling water inlet 313. The sterilizer chamber 305 also has a pure steam inlet 314 and a pure steam outlet 311 communicating with the sterilization chamber, as well as a compressed air inlet 308 and a compressed air outlet 318 communicating with the sterilization chamber. External industrial steam, cooling water, and pure steam are connected to their respective inlet pipes, and each outlet pipe is connected to an external discharge port.

[0029] After sterilization (actual sterilization process) is initiated, industrial steam enters the jacket through industrial steam inlet 312 to indirectly heat the sterilization chamber. When the chamber temperature reaches 90°C, pure steam is directly introduced into the sterilization chamber through pure steam inlet 314 to perform actual sterilization of the vessels and culture media. During sterilization, the condensate generated by the industrial steam and pure steam is discharged through industrial steam outlet 309 and pure steam outlet 311, respectively. After sterilization, when cooling the chamber, cooling water enters the jacket through cooling water inlet 313 to rapidly cool the sterilization chamber. The waste cooling water after heat exchange is discharged and recycled through cooling water outlet 310. Simultaneously, clean compressed air enters the chamber through compressed air inlet 308 for pressure maintenance, ventilation, and cooling. The hot air is discharged through compressed air outlet 318 until the temperature reaches the target temperature.

[0030] During the entire sterilization process, the first jacket temperature sensor 315, the second jacket temperature sensor 319, the first inner chamber temperature sensor 316, and the second inner chamber temperature sensor 320 monitor the temperature of the jacket and the sterilization chamber in real time, while the first jacket pressure sensor 321, the second jacket pressure sensor 322, the first inner chamber pressure sensor 323, and the second inner chamber pressure sensor 324 monitor the pressure of the jacket and the sterilization chamber in real time. Each detection element is connected to an external control system to ensure the controllability and safety of the sterilization process and to complete thorough sterilization.

[0031] After the sterilization process is completely completed, move the sterilizer to a Class A clean environment, loosen the fastening handwheel 304, remove the door bolt 303 from the door bolt slot 302, open the door 301, and remove the culture medium that has been sterilized and cooled from the fixed plate assembly 500.

[0032] Throughout the entire sterilization process, from the start to the end, the drive motor 201 drives the transmission mechanism through the drive shaft 202, causing the rotating frame assembly 400 to rotate continuously. The specific transmission process is described in detail in Example 2.

[0033] like Figure 2 As shown, in a preferred embodiment, the sterilizer chamber assembly 300 further includes a door 301 disposed on the side of the sterilizer chamber 305. One side of the door 301 is hinged to the sterilizer chamber 305, and the other side of the door 301 is provided with a door bolt groove 302. The sterilizer chamber 305 is provided with a door bolt 303 corresponding to the door bolt groove 302, and the door bolt 303 is provided with a movable fastening handwheel 304. In use, the operator closes the door 301, causing the door bolt 303 to engage in the door bolt groove 302, and locks the door 301 by rotating the fastening handwheel 304, ensuring reliable sealing of the chamber during sterilization.

[0034] like Figure 1 , Figure 2 , Figure 3 and Figure 9As shown, in a preferred embodiment, the rotating frame 406 includes a rotating shaft 411 and a rotating support disk 409 coaxially fixed to the outside of the rotating shaft 411. A second slot 410 corresponding to the planetary gear 404 is formed circumferentially on the rotating support disk 409, and a fixed support rod 416 is rotatably inserted into the second slot 410. A rotating frame top slot 307 and a rotating frame bottom slot 306 are respectively formed at the top and bottom of the sterilizer cavity 305. The top end of the rotating shaft 411 is rotatably installed in the rotating frame top slot 307, and the bottom end of the rotating shaft 411 is rotatably installed in the rotating frame bottom slot 306. A gear ring fixing groove 317 is formed on the inner wall of the sterilizer cavity 305, and a gear ring 403 is fixedly installed in the gear ring fixing groove 317.

[0035] like Figure 2 , Figure 4 , Figure 6 and Figure 11 As shown, in a preferred embodiment, the drive motor assembly 200 further includes a drive motor mounting bracket 204 and a sterilizer cavity support 206. The drive motor 201 is fixedly mounted inside the drive motor mounting bracket 204 by drive motor mounting bolts 205, and the drive motor mounting bracket 204 is disposed inside the sterilizer cavity support 206. The sterilizer cavity support 206 is fixedly connected to the bottom of the sterilizer cavity 305 by sterilizer cavity mounting bolts 207. A drive gear 203 is fixedly sleeved on the drive shaft 202 of the drive motor 201. The bottom end of the rotating frame 406 passes through the bottom of the sterilizer cavity 305, and a driven gear connector 413 is provided at the bottom end of the rotating shaft 411. A driven gear 405 is fixedly sleeved on the driven gear connector 413. The drive gear 203 and the driven gear 405 mesh with each other to realize the transmission connection between the drive motor 201 and the rotating frame 406.

[0036] In a preferred embodiment, multiple rotating support disks 409 are axially spaced and mounted on the outside of the rotating shaft 411, with each rotating support disk 409 forming a rotating disk mounting space. Multiple fixed disk assemblies 500 are circumferentially arranged on each rotating support disk 409, and multiple fixed disk assemblies 500 are axially spaced on each fixed support rod 416.

[0037] like Figure 1 , Figure 4 and Figure 5 As shown, in a preferred embodiment, the sterilizer further includes a movable platform assembly 100. The movable platform assembly 100 includes a platform 101, wheels 102 disposed at the bottom of the platform 101, brakes 103 disposed on the wheels 102, and a handle 104 disposed at one end of the platform 101. The sterilizer chamber assembly 300 is fixedly mounted on the platform 101, and the drive motor assembly 200 is mounted between the sterilizer chamber assembly 300 and the platform 101.

[0038] This embodiment describes the overall structure and working process of the sterilizer, wherein the planetary gear transmission structure between the rotating frame assembly 400 and the fixed plate assembly 500 is the core of realizing the compound motion of the vessels. Embodiment 2 below further details the specific assembly relationship and working method of this transmission structure.

[0039] Example 2 like Figures 6 to 13 As shown, this embodiment provides a culture medium support based on a planetary gear system, as a further refinement of the assembly relationship and transmission process of the rotating frame assembly 400 and the fixed disk assembly 500 in Embodiment 1.

[0040] In this embodiment, multiple layers of rotating support disks 409 are axially spaced and installed on the outside of the rotating shaft 411, with each rotating support disk 409 forming a rotating disk mounting space. Multiple second slots 410 are circumferentially formed on each rotating support disk 409, and the number and position of the second slots 410 on each layer of rotating support disk 409 correspond one-to-one with the number and position of the planetary gears 404 in the planetary gear assembly. The planetary gear assembly also includes a gear ring 403, which is fixedly installed on the inner wall of the sterilizer cavity 305 and remains stationary during operation, serving as a fixing component of the planetary gear system. A fixed support rod 416 is rotatably inserted into the second slot 410, and the fixed support rod 416 is coaxially and fixedly connected to the planetary gears 404, meaning that the fixed support rod 416 and the planetary gears 404 are relatively fixed in the circumferential direction and rotate synchronously. The fixed plate assembly 500 is fixedly sleeved on the fixed support rod 416, and the fixed plate assembly 500 is provided with multiple container fixing slots 502 along the circumference for placing various culture medium vessels such as bucket containers and petri dishes.

[0041] The transmission process in this embodiment is as follows: Throughout the entire sterilization process, from the start to the end, the drive shaft 202 of the drive motor 201 drives the drive gear 203 to rotate. The drive gear 203 meshes with the driven gear 405, thereby driving the rotating shaft 411 to rotate. The rotating shaft 411 then drives the entire rotating frame assembly 400 to start rotating.

[0042] After the rotating frame assembly 400 rotates, the rotating frame 406 drives the fixed support rod 416 and the fixed disk assembly 500 on it to revolve around the central axis of the sterilizer cavity 305. Simultaneously, since the gear ring 403 is fixedly installed on the inner wall of the sterilizer cavity 305 and remains stationary, the planetary gear 404 meshes with the internal teeth of the gear ring 403 during its revolution, forcing it to rotate around its own axis, thereby driving the fixed support rod 416 and the fixed disk assembly 500 fixedly fitted thereon to rotate synchronously. Thus, the containers placed in the container fixing slot 502 simultaneously experience both revolution around the central axis of the sterilizer cavity 305 and rotation around their own central axis.

[0043] In this embodiment, by cooperating with the rotating frame assembly, planetary gear assembly, and fixed disk assembly, only one drive motor is needed to drive all the vessels to simultaneously achieve a combined motion of revolution and rotation. This enhances the turbulence effect of the culture medium inside the vessels, effectively avoids component precipitation and system inhomogeneity, and also helps to improve the heating uniformity of various parts of the culture medium. Furthermore, the fixed disk assembly can simultaneously accommodate a batch of vessels, meeting the requirements for batch synchronous processing, and improving sterilization efficiency, sterilization qualification rate, and culture medium quality.

[0044] like Figure 8 , Figure 11 and Figure 12 As shown, in a preferred embodiment, the fixed disk assembly 500 is mounted on the fixed support rod 416 by engaging T-shaped slide rails and T-shaped keyways. Specifically, the outer surface of the turntable fixing part of the fixed support rod 416 has multiple first T-shaped slide rails 414 extending axially along the circumferential direction. The fixed disk assembly 500 includes a fixed disk body 501 and a first T-shaped keyway 503 disposed on the inner wall of the central hole of the fixed disk body 501. The first T-shaped keyway 503 is correspondingly disposed with the first T-shaped slide rails 414, and is used to engage with the first T-shaped slide rails 414 through the opening at the upper end of the first T-shaped slide rails 414, and slide downward along them until it abuts against the lower end of the first T-shaped slide rails 414. No additional fasteners are required, and disassembly and assembly are convenient and quick, facilitating the replacement of different types of fixed disk assemblies according to process requirements.

[0045] In a preferred embodiment, a small air inlet and ventilation hole is provided through the outer wall and bottom of the fixing plate body 501 of the fixing plate assembly 500 between it and the container fixing slot 502. During high-temperature and high-pressure sterilization, condensate can be discharged in time through this small hole, preventing liquid accumulation at the bottom of the container fixing slot 502; at the same time, pure steam can enter the container fixing slot 502 area through this small hole, so that the bottom and side walls of the vessel placed in the container fixing slot 502 can fully contact the pure steam, increasing the effective contact area between the vessel and the pure steam, thereby improving the uniformity of heating of various parts of the vessel and ensuring the consistency of sterilization effect.

[0046] like Figure 2 , Figure 8 , Figure 9 and Figure 10 As shown, in a preferred embodiment, the rotating shaft 411 has a first slot 408 circumferentially formed on the lower part of the fixed plate assembly 500. A lower bearing 401 of the rotating frame assembly is installed in the first slot 408, and the rotating shaft 411 is rotatably mounted on the bottom of the sterilizer cavity 305 via the lower bearing 401. A rotating frame assembly suspension slot 412 is provided at the top of the rotating shaft 411, and an upper bearing 402 of the rotating frame assembly is installed in the suspension slot 412. The top of the rotating shaft 411 is rotatably connected to the top of the sterilizer cavity 305 via the upper bearing 402.

[0047] like Figures 6 to 13 As shown, in a preferred embodiment, the fixed support rod 416 has a coaxially arranged turntable fixing part, a rotating engagement part, and a gear connecting part. The fixed disk assembly 500 is sleeved on the outside of the turntable fixing part, realizing the fixed connection between the fixed disk assembly 500 and the fixed support rod 416. The rotating engagement part is located at the bottom end of the turntable fixing part and has a support rod bearing slot along the circumference. A support rod bearing (such as the first support rod bearing 421, the second support rod bearing 426, and the third support rod bearing 431) is installed in the support rod bearing slot. The rotating engagement part is rotatably inserted into the second slot 410 through the support rod bearing. The gear connecting part has a planetary gear slot along the circumference. A planetary gear key is provided at the bottom of the planetary gear slot. The planetary gear 404 is fixedly engaged in the planetary gear slot through the planetary gear key, realizing the circumferential fixation between the planetary gear 404 and the fixed support rod 416.

[0048] Furthermore, both the top and bottom outer surfaces of the rotating mating part are provided with threads. In radial projection, the threads at both ends of the rotating mating part are located on the upper and lower sides of the rotating support disk 409, respectively, and fixing nuts (such as the first fixing nut 420 and the second fixing nut 422, the third fixing nut 425 and the fourth fixing nut 427, the fifth fixing nut 430 and the sixth fixing nut 432) are installed on the threaded sections at both ends. These nuts are used to achieve axial positioning of the fixing support rod 416 relative to the rotating support disk 409, prevent the fixing support rod from axially moving during rotation, and ensure the positional accuracy and stability of each layer of fixing disk assembly in the height direction.

[0049] like Figure 13 As shown, furthermore, both the top and bottom outer surfaces of the gear connection are provided with threads. In radial projection, the threads at both ends of the gear connection are located on the upper and lower sides of the planetary gear 404, respectively, and a seventh fixing nut 433 and an eighth fixing nut 434 are respectively installed on the threaded sections at both ends. These are used to axially limit the fixed support rod 416 relative to the planetary gear 404, prevent the planetary gear from sliding axially along the fixed support rod during transmission, and ensure the meshing accuracy and transmission reliability between the planetary gear and the gear ring.

[0050] Furthermore, such as Figure 9 and Figure 13As shown, the rotating support disk 409 comprises three layers. The fixed support rod 416 includes a first rod 419, a second rod 424, and a third rod 429 arranged coaxially from top to bottom. A first support rod bearing 421 is sleeved in the middle of the rotating engagement portion at the bottom of the first rod 419, and a first fixing nut 420 and a second fixing nut 422 are respectively fixedly installed at the top and bottom ends of the rotating engagement portion of the first rod 419. A second support rod bearing 426 is sleeved in the middle of the rotating engagement portion at the bottom of the second rod 424, and a third fixing nut 425 and a fourth fixing nut 427 are respectively fixedly installed at the top and bottom ends of the rotating engagement portion of the second rod 424. A second stud 423 is provided at the top of the turntable fixing portion of the second rod 424, and the second stud 423 is fixedly connected to the first threaded groove at the bottom end of the turntable fixing portion of the first rod 419. A third support rod bearing 431 is sleeved in the middle of the rotating mating part at the bottom of the third rod 429. A fifth fixing nut 430 and a sixth fixing nut 432 are respectively fixedly installed at the top and bottom of the rotating mating part of the third rod 429. A third stud 428 is provided at the top of the turntable fixing part of the third rod 429, and the third stud 428 is fixedly connected to the second threaded groove at the bottom of the turntable fixing part of the second rod 424. The rods are sequentially coaxially connected through the engagement of the threaded grooves and studs, facilitating layered assembly, disassembly, and maintenance. Simultaneously, it allows the rotation of each layer of the fixed disc assembly to proceed independently and smoothly without interference.

[0051] In a preferred embodiment, a first stud 418 is provided at the top of the uppermost turntable fixing part, and a plug 417 is fixedly installed on the first stud 418 to block the opening at the top of the uppermost fixing support rod, so as to facilitate the assembly and disassembly of the fixing plate assembly 500 on the uppermost turntable fixing part.

[0052] This embodiment describes the basic structure and transmission principle of the planetary gear train support. In practical applications, various load-bearing modules can be further integrated on the fixed support rod 416 to accommodate different types of culture media. Examples 3 and 4 below provide further detailed explanations of the staggered support for test tube slant culture media and the separate fixation for petri dishes, respectively.

[0053] Example 3 like Figure 8 , Figure 11 and Figures 14 to 18 As shown, this embodiment provides a staggered support bracket for culture medium containers, which is a further refinement of the staggered arrangement and slanted culture medium forming function on the fixed support rod 416 in embodiments 1 and 2.

[0054] In this embodiment, the fixed support rod 416 is provided with a first mounting position and a second mounting position spaced apart along the axial direction, wherein the second mounting position is higher than the first mounting position. A fixed disk assembly 500 is fixedly sleeved on the first mounting position of the fixed support rod 416. The fixed disk assembly 500 is provided with a plurality of container fixing slots 502 along the circumferential direction for fixing a barrel-type container 504. The barrel-type container 504 includes a barrel body and a barrel lid 505 fitted onto the barrel body. A rotating sleeve assembly 800 is fixedly sleeved on the second mounting position of the fixed support rod 416. A rotating connecting rod 807 is rotatably provided on the rotating sleeve assembly 800, and the rotating sleeve assembly 800 is provided with a transmission and engagement mechanism for driving the rotating connecting rod 807 to rotate around its own axis. An inclined plane fixing assembly 700 is fixedly installed on the end of the rotating connecting rod 807 for tilting and fixing the test tube 704.

[0055] In this embodiment, by installing the fixed plate assembly 500 and the rotating sleeve assembly 800 at different heights, the barrel-type container 504 and the inclined fixed test tube 704 are arranged vertically in a staggered manner so that they do not interfere with each other. This achieves simultaneous compatibility and support of two types of containers within a limited space, avoids batch sterilization, effectively improves sterilization efficiency, and has a compact structure and is easy to assemble.

[0056] The staggered support frame of this embodiment has flexible sterilization mode adaptability, enabling sterilization combinations of various vessel and culture medium types according to production needs. For barrel containers 504 and test tubes 704, the frame can support individual or simultaneous empty sterilization of both, as well as simultaneous or individual sterilization of liquid culture media in barrel containers and liquid culture media in test tubes. It can also support individual or simultaneous sterilization of liquid culture media in barrel containers and semi-solid or solid culture media in test tubes. Users can flexibly choose to place barrel containers 504 and / or test tubes 704 on the corresponding fixed tray assembly 500 and / or inclined fixed assembly 700 according to actual process requirements, completing the sterilization of single or multiple types of vessels and culture media within the same sterilization cycle without batch processing, further improving production efficiency and equipment utilization.

[0057] The installation and angle adjustment process of the inclined plane fixing component 700 in this embodiment is as follows: Test tube 704 is fully inserted into test tube hole 702 on inclined plate holder 701, and test tube plug 705 is inserted into the upper end of test tube 704 until it fits completely. The rotating connecting rod 807 is driven to rotate around its own axis to the target angle through the transmission and engagement mechanism, thereby driving inclined plate holder 701 and test tube 704 on it to rotate synchronously to the required tilt angle and then lock it, so that test tube 704 maintains this tilted posture during subsequent sterilization and culture medium solidification processes.

[0058] The specific operation method of the transmission and engagement mechanism is as follows: The operator moves the spur bevel gear lever 805, causing it to slide along the rotating groove 803, which drives the first spur bevel gear 901 to rotate. The first spur bevel gear 901 meshes with the second spur bevel gear 902 to rotate, thereby driving the rotating connecting rod 807 to rotate, so that the inclined frame 701 is tilted to the required angle. Then, the fixing nut 806 is tightened so that it abuts against the surface of the rotating sleeve body 801, thereby locking the position of the spur bevel gear lever 805 and fixing the inclined frame 701 accordingly.

[0059] Before and during sterilization, the culture medium in the test tubes is in a liquid state. After sterilization, as the chamber cools to the pouring temperature, the culture medium in the test tube solidifies directly into a slant at a set tilt angle, achieving integrated sterilization and slant formation. For test tubes containing semi-solid or solid culture media, heat-sensitive nutrients can be added as needed during the chamber cooling process after sterilization. Specifically, the operator can move the sterilizer to a Class A clean environment and drive the rotating connecting rod 807 to rotate via a transmission mechanism, keeping the slant holder 701 at the preset tilt angle. Heat-sensitive nutrients are added in batches as needed and stirred thoroughly. After addition, cooling continues to the pouring temperature, and the culture medium in the test tube solidifies directly into a slant at the set tilt angle, with the heat-sensitive nutrients evenly distributed in the slant culture medium, achieving integrated continuous operation of sterilization, heat-sensitive nutrient addition, and slant formation. All operations are completed within the same equipment, eliminating the need to transfer test tubes and avoiding the risk of contamination of sterilized culture media during transfer.

[0060] In practical applications, the number and size of the container fixing slots 502 on the fixing plate assembly 500 can be adapted to the specifications of the barrel-type container. The tilt angle of the test tube 704 on the inclined plane fixing assembly 700 can be adjusted by a transmission mechanism to adapt to the requirements of different microbial culture processes for the inclined plane angle.

[0061] like Figure 8 , Figure 12 , Figure 14 and Figure 15As shown, in a preferred embodiment, the outer surface of the fixed support rod 416 has multiple first T-shaped slide rails 414 circumferentially formed, and the first T-shaped slide rails 414 extend axially downward from their upper open ends to a first mounting position. The fixed disk assembly 500 includes a fixed disk body 501 and a first T-shaped key 503 disposed on the inner wall of the central hole of the fixed disk body 501. The first T-shaped key 503 is correspondingly disposed with the first T-shaped slide rails 414 and is used to engage with the first T-shaped slide rails 414 from their open ends and slide downward along them until it abuts against the lower end of the first T-shaped slide rails 414. The outer surface of the fixed support rod 416 also has multiple second T-shaped slide rails 415 circumferentially formed, and the second T-shaped slide rails 415 extend axially downward from their upper open ends to a second mounting position. The rotating sleeve assembly 800 includes a rotating sleeve body 801 and a second T-shaped key 804 disposed on the inner wall of the central hole of the rotating sleeve body 801. The second T-shaped key 804 is correspondingly disposed with a second T-shaped slide rail 415 and is used to engage with the second T-shaped slide rail 415 from its open end and slide downward along it until it abuts against the lower end of the second T-shaped slide rail 415. The mounting of the fixed plate assembly and the rotating sleeve assembly is achieved by the engagement of the T-shaped slide rail and the T-shaped key 804, without the need for additional fasteners, making assembly and disassembly convenient and quick.

[0062] Furthermore, multiple first T-shaped slide rails 414 and multiple second T-shaped slide rails 415 are staggered along the circumference of the fixed support rod 416, so that the fixed disk assembly 500 and the rotating sleeve assembly 800 have different positioning angles in the circumference, avoiding interference between the two during assembly, and at the same time helping to improve the overall structural strength and rigidity of the fixed support rod 416.

[0063] like Figures 16 to 18 As shown, in a preferred embodiment, the inclined plane fixing assembly 700 includes an inclined plane frame 701. The upper surface of the inclined plane frame 701 has multiple arrayed test tube holes 702 for inserting test tubes 704 at an angle and maintaining their inclined posture. A polygonal inclined plane frame connecting and fixing hole 703 (such as a quadrilateral hole) is provided on the side of the inclined plane frame 701 opposite to the rotating connecting rod 807. The end of the rotating connecting rod 807 is fixedly inserted into the inclined plane frame connecting and fixing hole 703, ensuring that the inclined plane frame can rotate synchronously when the rotating connecting rod rotates, resulting in reliable transmission and a simple structure.

[0064] like Figure 16 and Figure 17As shown, in a preferred embodiment, the transmission mechanism includes a spur bevel gear set 900 and a power input mechanism connected to the spur bevel gear set 900. The spur bevel gear set 900 includes a first spur bevel gear 901 and a second spur bevel gear 902. The first spur bevel gear 901 is fitted into a receiving groove in the rotating sleeve body 801 and is coaxially arranged with the rotating sleeve body 801. The second spur bevel gear 902 is received in the receiving groove of the rotating sleeve body 801 and meshes with the first spur bevel gear 901. The axes of the first spur bevel gear 901 and the second spur bevel gear 902 are perpendicular to each other. The power input mechanism is connected to the first spur bevel gear 901 and is used to drive the first spur bevel gear 901 to rotate. The rotating connecting rod 807 is fixedly connected to the second spur bevel gear 902 and is used to rotate under the drive of the second spur bevel gear 902. Specifically, the outer wall of the rotating sleeve body 801 has a radially extending fixing hole along its circumference. One end of the rotating connecting rod 807 is rotatably inserted into this fixing hole. The inner wall of the fixing hole cooperates with the outer circumferential wall of the rotating connecting rod 807 to achieve radial limiting and axial positioning of the rotating connecting rod 807, so that the rotating connecting rod 807 can only rotate around its own axis. The second spur bevel gear 902 is fixedly sleeved on the end of the rotating connecting rod 807, and the two are relatively fixed in the circumferential direction. When the first spur bevel gear 901 rotates under the drive of external force, the second spur bevel gear 902 meshing with it rotates accordingly. Since the axial and radial directions of the rotating connecting rod 807 are both positioned through the fixing hole, the second spur bevel gear 902 can only rotate around its own axis, thereby driving the rotating connecting rod 807 to rotate synchronously, accurately transmitting the rotational motion of the power input mechanism to the rotating connecting rod 807.

[0065] Furthermore, a rotating groove 803 extending along the rotation trajectory of the first spur bevel gear 901 is formed on the upper or lower surface of the rotating sleeve body 801. The power input mechanism includes a spur bevel gear lever 805 and a fixing nut 806. The spur bevel gear lever 805 is slidably disposed within the rotating groove 803, with one end located outside the rotating groove 803 for easy gripping by the operator, and the other end passing through the rotating groove 803 and fixedly connected to the first spur bevel gear 901. The fixing nut 806 is detachably installed on the end of the spur bevel gear lever 805 located outside the rotating groove 803, and is used to lock the position of the spur bevel gear lever 805 after the first spur bevel gear 901 has rotated to the target position. A second spur bevel gear key 903 is provided in the central hole of the second spur bevel gear 902, and a rotating connecting rod 807 passes through the central hole of the second spur bevel gear 902 and is fixedly connected to the second spur bevel gear 902 through the second spur bevel gear key 903.

[0066] Furthermore, there are multiple second spur bevel gears 902, which are arranged circumferentially along the first spur bevel gear 901. Correspondingly, each second spur bevel gear 902 is fixedly connected to a rotating connecting rod 807 and a corresponding inclined plane fixing component 700, thereby enabling one device to simultaneously drive multiple inclined plane fixing components 700 to synchronously adjust their angles, increasing the number of test tubes processed at one time and meeting the needs of mass production.

[0067] This embodiment describes how to achieve a staggered arrangement of test tube slant culture medium and bucket-type containers in the vertical direction. The following embodiment 4 provides a further detailed explanation of the separation and clamping fixation of the lid and bottom of petri dishes.

[0068] Example 4 like Figure 8 , Figure 11 , Figure 17 and Figures 19 to 24 As shown, this embodiment provides a split-type fixed petri dish sterilization support, which is a further refinement of the staggered arrangement of the fixed support rod 416 and the petri dish culture medium forming function in embodiments 1 and 2.

[0069] In this embodiment, the fixed support rod 416 is provided with a first mounting position and a second mounting position spaced apart along the axial direction, wherein the second mounting position is higher than the first mounting position. The fixed disk assembly 500 is fixedly sleeved on the first mounting position of the fixed support rod 416. The fixed disk assembly 500 is provided with a plurality of container fixing slots 502 along the circumferential direction for fixing the bottom 602 of the dish 601. The dish fixing assembly 600 includes a rotating sleeve assembly 800 and a chuck assembly 1000. The rotating sleeve assembly 800 is fixedly sleeved on the second mounting position of the fixed support rod 416. A rotating connecting rod 807 is rotatably provided on the rotating sleeve assembly 800, and the rotating sleeve assembly 800 is provided with a first transmission engagement mechanism for driving the rotating connecting rod 807 to rotate around its own axis. The chuck assembly 1000 is fixedly installed on the outside of the rotating sleeve assembly 800. The chuck assembly 1000 includes a radially openable and closable jaw assembly and a second transmission engagement mechanism that is transmissionally connected to the rotating connecting rod 807. The second transmission and engagement mechanism is used to drive the claw assembly to open and close under the rotation drive of the rotating connecting rod 807, so as to clamp the lid 603 of the flat dish 601.

[0070] In this embodiment, the process of gripping and fixing the lid 603 is as follows: The operator drives the rotating connecting rod 807 to rotate around its own axis via the first transmission mechanism. The rotational motion of the rotating connecting rod 807 is transmitted to the jaw assembly via the second transmission mechanism, causing the jaw assembly to move radially inward, thereby clamping the dish lid 603. Specifically, the movable rotating sleeve assembly 800 and the chuck assembly 1000 are connected and fixed by the chuck connecting fixing rod 1001, together forming the gripping and fixing device for the dish lid 603. The rotating connecting rod 807 drives the third spur bevel gear 1007 to rotate, and the third spur bevel gear 1007 meshes with the fourth spur bevel gear 1013 to rotate, thereby driving the vortex ridge 1014 to rotate. The vortex ridge 1014 pulls the vortex groove 1011 to rotate, which in turn pulls the transmission foot clamp 1005 and the flat dish foot clamp 1006 to open and close synchronously, realizing the clamping and releasing of the dish lid 603 by the jaw assembly. After sterilization, the dish lid 603 can be released by reversing the operation of the first transmission mechanism.

[0071] In this embodiment, the process of eliminating air from the plate is as follows: When air sterilization of the petri dish is required, the dish bottom 602 is placed in the container fixing slot 502, and the dish bottom 602 is fixed relative to the rotating support plate 409 through the container fixing slot 502. After the dish lid 603 is clamped and fixed by the chuck assembly 1000, the operator slides the chuck assembly 1000 together with the dish lid 603 upward along the fixed support rod 416 to the highest position (sliding through the first T-shaped slide rail 414), and then rotates the chuck assembly 1000 to slide it into the second T-shaped slide rail 415 and fix it. In this way, the dish bottom 602 and the dish lid 603 are vertically offset from each other, and the steam can fully contact all surfaces of the dish bottom and the dish lid, eliminating sterilization dead corners.

[0072] When sterilizing petri dishes containing culture medium, the medium is in a liquid state before and during sterilization. After cooling to the pouring temperature, the medium solidifies directly in the bottom 602 of the dish. For the addition of heat-sensitive nutrients, the sterilizer can be moved to a Class A clean laminar flow hood. The operation is performed when the temperature inside the sterilizer is greater than the pouring temperature but less than or equal to the heat-sensitive temperature. The chuck assembly 1000 picks up the dish lid 603, drips the heat-sensitive nutrient into the bottom 602 of the dish, and then closes the lid again, achieving integrated sterilization and shaping within the same equipment.

[0073] During the sterilization of semi-solid or solid culture media in air-sterilized and solid-sterilized petri dishes, the bottom 602 and the lid 603 of the dish are separated in batches by the chuck assembly 1000. This allows steam to fully contact all surfaces of the bottom and lid, eliminating sterilization dead zones and ensuring more thorough sterilization. After the semi-solid or solid culture media in the petri dishes are sterilized, during the cooling process, when the temperature drops to a temperature higher than the pouring temperature for heat-sensitive addition, the sterilizer can be moved to a Class A clean environment. The lid 603 is then picked up as needed by the chuck assembly 1000, and the required heat-sensitive nutrients are added in batches to the bottom 602 and stirred thoroughly. After addition, the lid 603 is replaced. After continuing to cool to the pouring temperature, the culture medium is automatically spread and solidified in the bottom 602, achieving integrated processing of petri dish sterilization, heat-sensitive nutrient addition, and solidification within the same equipment.

[0074] like Figure 8 , Figure 11 , Figure 12 , Figure 17 , Figure 19 and Figure 20 As shown, in a preferred embodiment, the outer surface of the fixed support rod 416 has multiple first T-shaped slide rails 414 circumferentially formed, and the first T-shaped slide rails 414 extend axially downward from their upper open ends to a first mounting position. The fixed disk assembly 500 includes a fixed disk body 501 and a first T-shaped key 503 disposed on the inner wall of the central hole of the fixed disk body 501. The first T-shaped key 503 is correspondingly disposed with the first T-shaped slide rails 414 and is used to engage with the first T-shaped slide rails 414 from their open ends and slide downward along them until it abuts against the lower end of the first T-shaped slide rails 414. The outer surface of the fixed support rod 416 also has multiple second T-shaped slide rails 415 circumferentially formed, and the second T-shaped slide rails 415 extend axially downward from their upper open ends to a second mounting position. The rotating sleeve assembly 800 includes a rotating sleeve body 801 and a second T-shaped key 804 disposed on the inner wall of the central hole of the rotating sleeve body 801. The second T-shaped key 804 is correspondingly disposed with the second T-shaped slide rail 415 and is used to engage the second T-shaped slide rail 415 from the open end of the second T-shaped slide rail 415 and slide downward along it until it abuts against the lower end of the second T-shaped slide rail 415.

[0075] like Figure 17 and Figure 21As shown, in a preferred embodiment, the first transmission mechanism includes a spur bevel gear set 900 and a power input mechanism that is transmissionally connected to the spur bevel gear set 900. The spur bevel gear set 900 includes a first spur bevel gear 901 and a second spur bevel gear 902. The first spur bevel gear 901 is fitted into a receiving groove in the rotating sleeve body 801 and is coaxially arranged with the rotating sleeve body 801. The second spur bevel gear 902 is received in the receiving groove of the rotating sleeve body 801 and meshes with the first spur bevel gear 901. The axes of the first spur bevel gear 901 and the second spur bevel gear 902 are perpendicular to each other. A rotating connecting rod 807 is fixedly connected to the second spur bevel gear 902 and rotates under the drive of the second spur bevel gear 902.

[0076] Furthermore, a rotating groove 803 extending along the rotation trajectory of the first spur bevel gear 901 is formed on the upper or lower surface of the rotating sleeve body 801. The power input mechanism includes a spur bevel gear lever 805 and a fixing nut 806. The spur bevel gear lever 805 is slidably disposed within the rotating groove 803, with one end located outside the rotating groove 803 and the other end passing through the rotating groove 803 and fixedly connected to the first spur bevel gear 901, for driving the first spur bevel gear 901 to rotate. The fixing nut 806 is detachably installed on the end of the spur bevel gear lever 805 located outside the rotating groove 803, for locking the spur bevel gear lever 805 after the first spur bevel gear 901 rotates to the target position. A second spur bevel gear key 903 is provided in the central hole of the second spur bevel gear 902, and a rotating connecting rod 807 passes through the central hole of the second spur bevel gear 902 and is fixedly connected to the second spur bevel gear 902 through the second spur bevel gear key 903.

[0077] like Figure 17 , Figure 22 , Figure 23 and Figure 24 As shown, in a preferred embodiment, the chuck assembly 1000 includes a chuck connecting and fixing rod 1001, a top cover 1002, a chuck body 1004, a second transmission and engagement mechanism, and a jaw assembly. The top cover 1002 is mounted on the top of the chuck body 1004, the second transmission and engagement mechanism is mounted in the internal assembly space of the chuck body 1004, and the jaw assembly is slidably disposed in a guide rail 1008 at the bottom of the chuck body 1004. A top cover connecting and fixing groove 1003 is provided on the side of the top cover 1002, one end of the chuck connecting and fixing rod 1001 is fixedly inserted into the top cover connecting and fixing groove 1003, and the other end is fixedly inserted into the chuck connecting and fixing hole 802 opened on the side of the rotary sleeve assembly 800.

[0078] Furthermore, the second transmission engagement mechanism includes a third spur bevel gear 1007 and a fourth spur bevel gear 1013. The fourth spur bevel gear 1013 is installed inside the chuck body 1004 and is coaxially arranged with the chuck body 1004. The third spur bevel gear 1007 is installed inside the chuck body 1004 and meshes with the fourth spur bevel gear 1013, with the axes of the third spur bevel gear 1007 and the fourth spur bevel gear 1013 perpendicular to each other. The outer end of the third spur bevel gear 1007 extends to the outside of the chuck assembly 1000 and is provided with a spur bevel gear connecting fixing hole 1018 fixedly connected to the end of the rotating connecting rod 807, for driving the third spur bevel gear 1007 to rotate under the rotation drive of the rotating connecting rod 807. The bottom of the fourth spur bevel gear 1013 is provided with a vortex 1014 along the circumferential direction, and the top of the pawl assembly is provided with a vortex groove 1011 corresponding to the vortex 1014. The vortex groove 1011 and the vortex 1014 engage with each other and are used to drive the pawl assembly to move linearly in the guide rail 1008 under the rotation drive of the fourth spur bevel gear 1013.

[0079] Furthermore, the claw assembly includes a transmission foot clip 1005 and a plate clip 1006. The plate clip 1006 is engaged with the bottom of the transmission foot clip 1005, and a connecting fixing block 1009 is inserted between the plate clip 1006 and the transmission foot clip 1005 along a direction perpendicular to the sliding direction to achieve circumferential fixation between the two. The clamping surface of the plate clip 1006 is provided with a plate clip clamping ridge 1012 for abutting against the plate lid 603 to increase the friction during clamping and prevent the plate lid from slipping off during sterilization. Guide grooves 1016 are provided on both sides of the transmission foot clip 1005 to accommodate the protruding structure on the side of the guide rail 1008, forming an axial limit for the transmission foot clip 1005 relative to the chuck body 1004, ensuring that the claw assembly always moves along the guide rail direction during sliding and will not deviate or derail.

[0080] Furthermore, the chuck body 1004 includes an annular assembly space. The inner and outer sides of the annular assembly space are respectively an inner assembly portion and an outer assembly portion for assembly with the top cover 1002. The top surface of the outer assembly portion has a plurality of first fixing screw holes 1015 arranged circumferentially, and the top surface of the inner assembly portion has a plurality of second fixing screw holes 1017 arranged circumferentially. Both the first fixing screw holes 1015 and the second fixing screw holes 1017 are used for fixed connection with corresponding screw holes on the top cover 1002 via bolts. The second fixing screw hole 1017 is a through hole, and a fixing cap 1010 is provided at its bottom.

[0081] like Figure 8 and Figure 10As shown, in a preferred embodiment, a first stud 418 is provided at the top of the fixed support rod 416, and a plug 417 is fixedly installed on the first stud 418. The plug 417 can serve as a limiting member at the upper end of the T-shaped slide rail to prevent the fixed plate assembly or the rotating sleeve assembly from slipping off from the top during assembly or use.

[0082] Examples 2 to 4 above refine the internal support structure of the sterilizer in Example 1 from three dimensions: planetary gear transmission, staggered layered support of test tube slant surfaces, and separate fixation of flat plates. The structures described in each example can be used individually or in combination within the same sterilizer to adapt to the needs of different culture medium types and sterilization processes.

[0083] In the description of this application, it should be understood that the terms "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application.

[0084] Specific embodiments of the present invention have been described above. It should be understood that the present invention is not limited to the specific embodiments described above, and those skilled in the art can make various changes or modifications within the scope of the claims, which do not affect the essence of the present invention. Unless otherwise specified, the embodiments and features described in this application can be arbitrarily combined with each other.

Claims

1. A culture medium sterilizer with dynamic mixing function, characterized in that, include: The sterilizer cavity assembly (300) includes a sterilizer cavity (305), the interior of which forms a sterilization chamber; A rotating frame assembly (400) is disposed inside the sterilizer cavity (305). The rotating frame assembly (400) includes a rotating frame (406), a fixed support rod (416), and a planetary gear assembly. The gear ring (403) of the planetary gear assembly is fixedly disposed relative to the sterilizer cavity (305), and the fixed support rod (416) is rotatably disposed in the rotating frame (406) and is coaxially fixedly connected to the planetary gear (404) of the planetary gear assembly. The fixed plate assembly (500) is fixedly installed on the fixed support rod (416), and the fixed plate assembly (500) is provided with a container fixing slot (502) for fixing the vessel along the circumferential direction. The drive motor assembly (200) is connected to the rotating frame (406) in a transmission connection.

2. The culture medium sterilizer with dynamic mixing function according to claim 1, characterized in that, The rotating frame (406) includes a rotating shaft (411) and a rotating support disk (409) coaxially fixedly installed outside the rotating shaft (411). The rotating support plate (409) has a second slot (410) corresponding to the planetary gear (404) along its circumferential direction, and the fixed support rod (416) is rotatably inserted into the second slot (410); the top and bottom of the sterilizer cavity (305) are respectively provided with a rotating frame top slot (307) and a rotating frame bottom slot (306). The top end of the rotating shaft (411) is rotatably installed in the top slot (307) of the rotating frame, and the bottom end of the rotating shaft (411) is rotatably installed in the bottom slot (306) of the rotating frame; a toothed ring fixing groove (317) is provided on the inner wall of the sterilizer cavity (305), and the toothed ring (403) is fixedly installed in the toothed ring fixing groove (317).

3. The culture medium sterilizer with dynamic mixing function according to claim 1, characterized in that, The sterilizer chamber assembly (300) also includes a door (301) disposed on the side of the sterilizer chamber (305); One side of the door (301) is hinged to the sterilizer cavity (305), and the other side of the door (301) is provided with a door bolt groove (302). The sterilizer cavity (305) is provided with a door bolt (303) corresponding to the door bolt groove (302), and the door bolt (303) is provided with a movable fastening handwheel (304).

4. The culture medium sterilizer with dynamic mixing function according to claim 1, characterized in that, The outer wall of the sterilizer cavity (305) is provided with a jacket for the flow of heat exchange medium; The sterilizer cavity (305) is provided with an industrial steam inlet (312) and an industrial steam outlet (309) communicating with the jacket, as well as a cooling water inlet (313) and a cooling water outlet (310) communicating with the jacket. The sterilizer cavity (305) is also provided with a pure steam inlet (314) and a pure steam outlet (311) communicating with the sterilization chamber, as well as a compressed air inlet (308) and a compressed air outlet (318) communicating with the sterilization chamber.

5. The culture medium sterilizer with dynamic mixing function according to claim 4, characterized in that, The sterilizer cavity (305) is provided with a first jacket temperature sensor (315) and a second jacket temperature sensor (319) for detecting jacket temperature, a first inner chamber temperature sensor (316) and a second inner chamber temperature sensor (320) for detecting sterilization inner chamber temperature, a first jacket pressure sensor (321) and a second jacket pressure sensor (322) for detecting jacket pressure, and a first inner chamber pressure sensor (323) and a second inner chamber pressure sensor (324) for detecting sterilization inner chamber pressure.

6. The culture medium sterilizer with dynamic mixing function according to claim 1, characterized in that, The drive motor assembly (200) also includes a drive motor mounting bracket (204) and a sterilizer cavity support (206). The drive motor (201) is fixedly installed inside the drive motor mounting bracket (204) by drive motor fixing bolts (205), and the drive motor mounting bracket (204) is located inside the sterilizer cavity support (206); The sterilizer cavity support (206) is fixedly connected to the bottom of the sterilizer cavity (305) by the sterilizer cavity fixing bolt (207), and the bottom end of the rotating frame (406) passes through the bottom of the sterilizer cavity (305) and is connected to the drive shaft (202) of the drive motor (201) for transmission.

7. The culture medium sterilizer with dynamic mixing function according to claim 2, characterized in that, The rotating shaft (411) is equipped with multiple rotating support disks (409) spaced apart along the axial direction, and each rotating support disk (409) forms a rotating disk installation space. Each rotating support disk (409) is provided with a plurality of fixed disk assemblies (500) along the circumferential direction, and each fixed support rod (416) is provided with a plurality of said fixed disk assemblies (500) at intervals along the axial direction.

8. The culture medium sterilizer with dynamic mixing function according to claim 1, characterized in that, It also includes a mobile platform assembly (100); The mobile platform assembly (100) includes a platform (101), wheels (102) disposed at the bottom of the platform (101), brakes (103) disposed on the wheels (102), and a handle (104) disposed at one end of the platform (101). The sterilizer cavity assembly (300) is fixedly installed on the platform (101), and the drive motor assembly (200) is installed between the sterilizer cavity assembly (300) and the platform (101).

9. The culture medium sterilizer with dynamic mixing function according to claim 1, characterized in that, The fixed support rod (416) is provided with a first mounting position and a second mounting position spaced apart along the axial direction, wherein the second mounting position is higher than the first mounting position; The fixed plate assembly (500) is fixedly sleeved on the first mounting position of the fixed support rod (416) for fixing the barrel-type container (504). A rotating sleeve assembly (800) is fixedly sleeved on the second mounting position. A rotating connecting rod (807) is rotatably provided on the rotating sleeve assembly (800), and the rotating sleeve assembly (800) is provided with a transmission engagement mechanism for driving the rotating connecting rod (807) to rotate around its own axis. The end of the rotating connecting rod (807) is fixedly installed with a slope fixing component (700), which is used to fix the test tube (704) at an angle.

10. The culture medium sterilizer with dynamic mixing function according to claim 1, characterized in that, The fixed support rod (416) is provided with a first mounting position and a second mounting position spaced apart along the axial direction, wherein the second mounting position is higher than the first mounting position; The fixed plate assembly (500) is fixedly sleeved on the first mounting position of the fixed support rod (416) for fixing the bottom (602) of the plate (601). A rotating sleeve assembly (800) is fixedly sleeved on the second mounting position. A rotating connecting rod (807) is rotatably provided on the rotating sleeve assembly (800), and the rotating sleeve assembly (800) is provided with a first transmission engagement mechanism for driving the rotating connecting rod (807) to rotate around its own axis. A chuck assembly (1000) is fixedly installed on the outside of the rotating sleeve assembly (800). The chuck assembly (1000) includes a radially openable jaw assembly and a second transmission engagement mechanism that is pulsatorically connected to the rotating connecting rod (807). The second transmission engagement mechanism is used to drive the jaw assembly to open and close under the rotation drive of the rotating connecting rod (807) to clamp the lid (603) of the plate (601).

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