Hydrogen peroxide plasma and ultraviolet light synergistic sterilization evaluation system and method

Abstract

The application provides a hydrogen peroxide plasma and ultraviolet synergistic sterilization evaluation system and method, and belongs to the technical field of low-temperature sterilization evaluation. The system is composed of a sterilization module, a detection module, an opening and closing communication module and an operation evaluation module. The operation evaluation module is in bidirectional communication with each sterilization unit, the fluorescence detection box, the opening and closing communication module and the moving base. The sealed box realizes airtight isolation and intercommunication with the fluorescence detection box through the opening and closing communication module, and the moving base carries the standard bacterial film back and forth between the two warehouses. When working, the materials and the bacterial film are sealed into the box, the plasma is pre-activated by low-power discharge, and then the hydrogen peroxide nano steam is conveyed. The concentration is monitored in real time by the sensor array, and after reaching the standard, the high-power plasma and the double ultraviolet unit are opened synchronously for synergistic sterilization. At the end of sterilization, the equipment is turned off, the communication module is opened to transport the bacteria to the fluorescence detection box to collect live bacteria data, and the whole process of ternary synergistic sterilization is realized. The automatic closed loop and the real-time quantitative evaluation of the sterilization effect are realized.

Description

Technical Field

[0001] This invention belongs to the field of low-temperature sterilization evaluation technology, specifically relating to a hydrogen peroxide plasma and ultraviolet synergistic sterilization evaluation system and method. Background Technology

[0002] Medical precision endoscopes, polymer consumables, and other heat-sensitive medical devices commonly employ low-temperature hydrogen peroxide sterilization processes. The determination of sterilization compliance directly impacts clinical infection control safety. Sterilization assurance levels are a rigid compliance standard. Relying solely on post-sterilization culture is time-consuming. There is an urgent need for an integrated assessment method that can simultaneously collect sterilization data and quantitatively couple sterilization factors. This would allow for accurate determination of the true sterilization effectiveness and differentiation between sterilization using a single medium and those using multiple factors in synergy.

[0003] In the prior art, see Chinese invention patent application number "202310761061.9", which discloses a single hydrogen peroxide sterilization load assessment scheme. By collecting load characteristics such as instrument material and lumen, and combining temperature and concentration sample data, an assessment spectrum is constructed. The optimal sterilization dose is solved by relying on a genetic algorithm. Load loss and dose calculation are performed only for a single hydrogen peroxide medium.

[0004] However, with the development of multi-media synergistic sterilization technology, existing evaluation methods are difficult to adapt to multi-media synergistic sterilization systems, which is not conducive to simultaneous online viable bacteria detection and sterilization assurance level calculation during the sterilization process. Summary of the Invention

[0005] The technical problem to be solved by this invention is how to evaluate the sterilization effect of a multi-element synergistic sterilization system. In view of the shortcomings of the prior art, this invention provides a system and method for evaluating the synergistic sterilization of hydrogen peroxide plasma and ultraviolet light.

[0006] To solve the above-mentioned technical problems, the technical solution adopted by the present invention is as follows:

[0007] In a first aspect, the present invention provides a hydrogen peroxide plasma and ultraviolet light synergistic sterilization evaluation system, comprising a sterilization module, a detection module, an opening and closing communication module, and a calculation and evaluation module; the sterilization module includes a sealed box and a hydrogen peroxide dissipation unit, a plasma generation unit, an ultraviolet light emission unit, and a sensing array unit built into the sealed box; the detection module includes a fluorescence detection box, and the sealed box and the fluorescence detection box are connected through the opening and closing communication module, so that a movable base carrying a standard spore strip in the sealed box can be moved into the detection box or returned to the sealed box;

[0008] The computational evaluation module is communicatively connected to the hydrogen peroxide dispersing unit, the plasma generating unit, the ultraviolet light emitting unit, the induction array unit, the fluorescence detection box, the opening and closing communication module, and the mobile base, and is used for:

[0009] After the material to be sterilized and the standard spore tablets are placed in the sealed box, the sealed box is closed, and the plasma generating unit is driven to construct a primary active environment by a preset low-power discharge.

[0010] The hydrogen peroxide dispersion unit is driven to output nano vapor, and the hydrogen peroxide concentration is collected by the induction array unit. When the hydrogen peroxide concentration reaches the preset concentration, the ultraviolet light emission unit is driven to turn on, and the plasma generation unit is synchronously adjusted to the preset high power.

[0011] The hydrogen peroxide emitting unit, the ultraviolet light emitting unit, and the plasma generating unit are driven to shut down after a preset sterilization time, the opening and closing communication module is driven to open, and the moving base is driven to move into the fluorescence detection box through the opening and closing communication module. The viable bacteria data of the standard spore strip are detected through the fluorescence detection box, and the sterilization evaluation result is calculated.

[0012] Compared with the prior art, the beneficial effects of the hydrogen peroxide plasma and ultraviolet synergistic sterilization evaluation system of the present invention include: the system is composed of a sterilization module, a detection module, an opening and closing communication module, and a calculation and evaluation module. The calculation and evaluation module establishes bidirectional communication with the hydrogen peroxide dissipation unit, the plasma generation unit, the ultraviolet light emission unit, the induction array unit, the fluorescence detection box, the opening and closing communication module, and the moving base. The sealed box is separated and sealed from the fluorescence detection box through the opening and closing communication module. The moving base acts as a carrier for transporting bacterial sheets and moves back and forth between the two chambers. In this way, during the operation, after the materials and bacterial tablets are sealed in the sealed box, the calculation and evaluation module first controls the plasma generation unit to discharge at low power to build a pre-activated active environment, then starts the hydrogen peroxide dispersion unit to deliver nano vapor, and the induction array unit provides real-time feedback on the concentration. After the standard is met, the plasma is switched to high power and the ultraviolet light emission unit is turned on. The three work together to sterilize. Finally, after the collaborative time ends, all sterilization units are shut down, the opening and closing connection module is opened, and the moving base is driven to carry the bacterial tablets into the fluorescence detection box to collect live bacteria data. Finally, the entire set of sterilization evaluation results is calculated and output, thereby realizing a fully automatic closed-loop sterilization process and real-time quantitative evaluation of the collaborative effect.

[0013] Optionally, the sealed box also has a built-in partition. The hydrogen peroxide dispersing unit includes a gas distribution network, a storage tank, a delivery pump, and a flash evaporator. The edge of the partition is detachably connected to the inner wall of the sealed box, and there are multiple partitions arranged in a cross pattern to separate multiple isolation chambers within the sealed box for the classified placement of the materials to be sterilized. The gas distribution network is evenly distributed on the inner wall of the sealed box, and multiple air outlets are evenly distributed on the pipe wall. The storage tank, delivery pump, and flash evaporator are connected in sequence and are all located outside the sealed box. The flash evaporator is connected to the air inlet of the gas distribution network located on the side wall of the sealed box, and both the flash evaporator and the delivery pump are communicatively connected to the calculation and evaluation module.

[0014] Optionally, the sealed box also includes a built-in catalytic degradation box and an exhaust valve. The catalytic degradation box is installed against the inner wall of the sealed box. There are two exhaust valves, both communicatively connected to the computational evaluation module. One exhaust valve is installed in the air outlet on the side wall of the sealed box and communicates with the interior of the catalytic degradation box. The other exhaust valve is installed in the air inlet of the catalytic degradation box away from the inner wall of the sealed box. The catalytic degradation box is filled with nano-manganese dioxide catalytic material. The computational evaluation module is also used for:

[0015] After the hydrogen peroxide emission unit, the ultraviolet light emission unit, and the plasma generation unit are turned off, and before the opening and closing communication module is turned on, the exhaust valve is driven to open. When the hydrogen peroxide concentration is lower than the preset concentration, the exhaust valve is closed.

[0016] Optionally, the plasma generating unit includes a plasma electrode, a water-cooling heat dissipation assembly, and a dual-stage radio frequency plasma generator. The inner wall of the sealed box is provided with an embedding groove, and the plasma electrode is embedded in the embedding groove. The water-cooling heat dissipation assembly and the dual-stage radio frequency plasma generator are both located outside the sealed box and are both communicatively connected to the computational evaluation module. The dual-stage radio frequency plasma generator is connected to the water-cooling heat dissipation assembly and electrically connected to the plasma electrode.

[0017] Optionally, the ultraviolet light emitting unit includes a power supply element, an integrated fixed lamp assembly, and a protective cover. The inner wall of the sealed box is hinged with a mounting base, the integrated fixed lamp assembly is mounted on the mounting base, and the protective cover covers the integrated fixed lamp assembly. The integrated fixed lamp assembly includes 254nm UVC mercury lamps and 275nm deep ultraviolet LEDs arranged at intervals. The power supply element is located outside the sealed box and is electrically connected to the integrated fixed lamp assembly and the computational evaluation module, respectively.

[0018] Optionally, the ultraviolet light emitting unit further includes a photocatalytic substrate. There are multiple photocatalytic substrates, which are spaced apart on the inner wall of the sealed box and are all located within the light irradiation range of the integrated fixed lamp group. The photocatalytic substrate has a porous honeycomb structure and is covered with a composite photocatalytic functional coating. The composite photocatalytic functional coating is formed by upconversion nanoparticles and graphene quantum dots through low-temperature sintering and uniformly loading them onto the inner and outer pore surfaces of the porous honeycomb structure.

[0019] Optionally, the sensing array unit includes an integrated base and a vacuum sensor, a temperature sensor, a hydrogen peroxide concentration sensor, a dual-band ultraviolet radiation sensor, and an active particle density sensor, all mounted on the integrated base. There are multiple integrated bases, which are evenly distributed on the inner wall of the sealed box and are all communicatively connected to the computation and evaluation module.

[0020] Optionally, the detection module further includes a fluorescence signal acquisition unit and a fluorescence spot imaging unit built into the fluorescence detection box, both of which are communicatively connected to the computation and evaluation module. When the movable base moves into the fluorescence detection box, both the fluorescence signal acquisition unit and the fluorescence spot imaging unit are oriented towards the movable base. The computation and evaluation module is further used for:

[0021] The standard spore-forming tablets on the mobile base are immersed in fluorescent buffer solution sprayed by the fluorescent signal acquisition unit. After being left to stand for a preset activation time, the characteristic fluorescent signals on the standard spore-forming tablets are continuously acquired.

[0022] The fluorescence dot imaging unit synchronously captures a full-area fluorescence dot distribution image of the standard spore-forming sheet. Based on the characteristic fluorescence signal and the full-area fluorescence dot distribution image, the real-time fluorescence intensity of the fluorescence dots is obtained. A pre-stored calibration curve fitting the live bacteria and fluorescence intensity is retrieved. The number of residual live bacteria is obtained based on the real-time fluorescence intensity and combined with the pre-input initial number of live bacteria of the standard spore-forming sheet to form the live bacteria data.

[0023] Optionally, the detection module further includes a process data storage unit, which contains theoretical data on the sterilization process and is communicatively connected to the calculation and evaluation module. The calculation and evaluation module is further used for:

[0024] The sterility assurance level value of the standard spore tablet is obtained based on the live bacteria data. When the sterility assurance level value is greater than a preset threshold, the sterilization process is deemed to have met the standard.

[0025] All data collected by the induction array unit during the sterilization process are retrieved, invalid data are removed, and the data are aligned with the time axis to form a synchronous time-series curve dataset with time as a function of hydrogen peroxide concentration, discharge power, and irradiation intensity.

[0026] Based on the synchronous time-series curve dataset, the process data storage unit retrieves multiple sets of theoretical sterility guarantee levels for single sterilization media under the same sterilization conditions, including hydrogen peroxide, plasma, and ultraviolet light. The maximum value of the multiple sets of theoretical sterility guarantee levels is taken as a comparison benchmark. The quotient obtained by dividing the difference between the sterility guarantee level value and the comparison benchmark by the comparison benchmark is used as the synergistic sterilization gain coefficient.

[0027] When the synergistic sterilization gain coefficient is greater than zero, it is determined that the synergistic sterilization effect is better than the sterilization effect of a single sterilization medium, and the sterilization evaluation result is generated.

[0028] Secondly, the present invention also provides a method for evaluating the synergistic sterilization of hydrogen peroxide plasma and ultraviolet light, comprising:

[0029] S1. After the materials to be sterilized and the standard spore tablets are placed in the sealed box of the sterilization module of the hydrogen peroxide plasma and ultraviolet synergistic sterilization evaluation system, the sealed box is closed, and the plasma generation unit of the sterilization module is driven to construct a primary active environment by a preset low-power discharge.

[0030] S2. Drive the hydrogen peroxide emission unit of the sterilization module to output nano vapor, collect the hydrogen peroxide concentration through the sensing array unit of the sterilization module, and when the hydrogen peroxide concentration reaches the preset concentration, drive the ultraviolet light emission unit of the sterilization module to turn on, and synchronously adjust the plasma generation unit of the sterilization module to the preset high power.

[0031] S3. Drive the hydrogen peroxide emitting unit, the ultraviolet light emitting unit, and the plasma generating unit to shut down after synchronously running for a preset sterilization time. Drive the communication module of the hydrogen peroxide plasma and ultraviolet synergistic sterilization evaluation system to open. At the same time, drive the movable base in the sealed box to move into the fluorescence detection box of the detection module of the hydrogen peroxide plasma and ultraviolet synergistic sterilization evaluation system through the opening and closing communication module. Detect the viable bacteria data of the standard spore strip through the fluorescence detection box and calculate to obtain the sterilization evaluation result.

[0032] Compared with the prior art, the beneficial effects of the hydrogen peroxide plasma and ultraviolet synergistic sterilization evaluation method of the present invention are the same as those of the hydrogen peroxide plasma and ultraviolet synergistic sterilization evaluation system described above, and will not be repeated here. Attached Figure Description

[0033] The present invention will now be described in further detail with reference to the accompanying drawings.

[0034] Figure 1 : A schematic diagram of the connection between the hydrogen peroxide plasma and ultraviolet light synergistic sterilization evaluation system in this embodiment of the invention;

[0035] Figure 2 : A schematic flowchart of the evaluation method for synergistic sterilization of hydrogen peroxide plasma and ultraviolet light in this embodiment of the invention;

[0036] Among them, 1-sterilization module, 11-sealed box, 12-hydrogen peroxide dispersion unit, 13-plasma generation unit, 14-ultraviolet emission unit, 15-induction array unit, 2-detection module, 21-fluorescence detection box, 22-fluorescence signal acquisition unit, 23-fluorescence spot imaging unit, 24-process data storage unit, 3-opening and closing connection module, and 4-operation evaluation module. Detailed Implementation

[0037] To better understand the present invention, the following embodiments further illustrate the content of the invention, but the scope of protection of the present invention is not limited to the following embodiments. Numerous specific details are set forth in the following description to provide a more thorough understanding of the invention. However, it will be apparent to those skilled in the art that the present invention can be practiced without one or more of these details.

[0038] The term "comprising" and its variations as used herein are open-ended, meaning "including but not limited to"; the term "based on" means "at least partially based on"; the term "one embodiment" means "at least one embodiment"; the term "another embodiment" means "at least one additional embodiment"; the term "some embodiments" means "at least some embodiments"; and the term "optionally" means "optional embodiments". Definitions of other terms will be given in the following description. It should be noted that the concepts of "first," "second," etc., mentioned in this invention are used only to distinguish different devices, modules, or units, and are not intended to limit the order of functions performed by these devices, modules, or units or their interdependencies.

[0039] It should be noted that the terms "a" and "a plurality of" used in this invention are illustrative rather than restrictive. Those skilled in the art should understand that, unless otherwise expressly indicated in the context, they should be understood as "one or more".

[0040] In a first aspect, an embodiment of the present invention provides a hydrogen peroxide plasma and ultraviolet light synergistic sterilization evaluation system, comprising a sterilization module 1, a detection module 2, an opening and closing communication module 3, and a computational evaluation module 4; the sterilization module 1 includes a sealed box 11 and a hydrogen peroxide dissipation unit 12, a plasma generation unit 13, an ultraviolet light emission unit 14, and an induction array unit 15 built into the sealed box 11; the detection module 2 includes a fluorescence detection box 21, and the sealed box 11 and the fluorescence detection box 21 are connected through the opening and closing communication module 3, so that a movable base carrying a standard spore strip in the sealed box 11 can be moved into or back to the sealed box 11; the computational evaluation module 4 is connected to the hydrogen peroxide dissipation unit 12, the plasma generation unit 13, the ultraviolet light emission unit 14, the induction array unit 15, the fluorescence detection box 21, the opening and closing communication module 3, and the movable base, respectively. The communication connection is used for: after the materials to be sterilized and the standard spore tablets are placed in the sealed box 11, sealing the sealed box 11, driving the plasma generating unit 13 to discharge at a preset low power to construct a primary active environment; driving the hydrogen peroxide dispersing unit 12 to output nano vapor, collecting the hydrogen peroxide concentration through the sensing array unit 15, and driving the ultraviolet light emitting unit 14 to turn on when the hydrogen peroxide concentration reaches the preset concentration, and synchronously adjusting the plasma generating unit 13 to the preset high power; driving the hydrogen peroxide dispersing unit 12, the ultraviolet light emitting unit 14 and the plasma generating unit 13 to turn off after synchronously running the preset sterilization time, driving the opening and closing communication module 3 to turn on, and simultaneously driving the moving base to move into the fluorescence detection box 21 through the opening and closing communication module 3, and detecting the viable bacteria data of the standard spore tablets through the fluorescence detection box 21, and calculating and obtaining the sterilization evaluation result.

[0041] Specifically, the sealed box 11 can be integrally welded from 8mm thick 316L stainless steel, with a sliding metal base inside. The base slot can simultaneously hold two standard spore tablets, ATCC9372 and ATCC7953. The hydrogen peroxide dispersing unit 12 is responsible for providing nano-sterilizing steam, the plasma generating unit 13 generates active free radicals, and the ultraviolet light emitting unit 14 outputs sterilizing ultraviolet light. The opening and closing communication module 3 establishes a separable conveying channel between the sealed box 11 and the fluorescence detection box 21. The opening and closing communication module 3 can adopt a double-layer airtight gate valve plus quartz sealed conveying pipe structure. The gate valve is driven by a stepper motor to completely isolate the gas communication between the two chambers. The calculation and evaluation module 4 is the central control calculation core of the whole machine.

[0042] In this embodiment, as Figure 1As shown, a hydrogen peroxide plasma and ultraviolet light synergistic sterilization evaluation system is set up, consisting of sterilization module 1, detection module 2, opening and closing communication module 3, and calculation and evaluation module 4. The calculation and evaluation module 4 establishes bidirectional communication with hydrogen peroxide dissipation unit 12, plasma generation unit 13, ultraviolet light emission unit 14, induction array unit 15, fluorescence detection box 21, opening and closing communication module 3, and mobile base via RS485 industrial bus. The sealed box 11 is detachably and sealedly connected to the fluorescence detection box 21 via the opening and closing communication module 3. The mobile base acts as a carrier for transporting bacterial sheets and moves back and forth between the two compartments. With this setup, during operation, after the materials and bacterial sheets are sealed in the sealed box 11, the calculation and evaluation module 4 first controls the plasma generating unit 13 to discharge at low power to build a pre-activated active environment, then starts the hydrogen peroxide dispersing unit 12 to deliver nano vapor, and the sensing array unit 15 provides real-time feedback on the concentration. Once the target is met, the plasma is switched to high power and the ultraviolet light emitting unit 14 is turned on. The three work together to sterilize. Finally, after the collaborative time ends, all sterilization units are shut down, the opening and closing connection module 3 is opened, and the moving base is driven to carry the bacterial sheets into the fluorescence detection box 21 to collect live bacteria data. Finally, the entire sterilization evaluation result is calculated and output, thereby realizing a fully automatic closed-loop sterilization process and real-time quantitative evaluation of the collaborative effect.

[0043] Optionally, the sealed box 11 also has a built-in partition. The hydrogen peroxide dissipation unit 12 includes a gas distribution network, a storage tank, a delivery pump, and a flash evaporator. The edge of the partition is detachably connected to the inner wall of the sealed box 11, and there are multiple partitions arranged in a cross pattern to separate multiple isolation chambers for the classified placement of materials to be sterilized within the sealed box 11. The gas distribution network is evenly distributed on the inner wall of the sealed box 11, and multiple air outlets are evenly distributed on the pipe wall. The storage tank, delivery pump, and flash evaporator are connected in sequence and are all located outside the sealed box 11. The flash evaporator is connected to the air inlet of the gas distribution network located on the side wall of the sealed box 11, and both the flash evaporator and the delivery pump are communicatively connected to the calculation and evaluation module 4.

[0044] Specifically, the partition can be made of snap-on 316L stainless steel sheet with Φ3mm uniform flow holes and a single layer thickness of 2mm. It can be freely disassembled and the size of the chamber can be adjusted to accommodate two different loads: endoscopes and surgical instruments. The flash evaporator has a built-in spiral heat exchange coil and an external 120℃ constant temperature electric heating jacket. The storage tank is a 50L corrosion-resistant PE sealed storage tank, equipped with a static pressure level sensor.

[0045] In this optional embodiment, the sealed box 11 also has a built-in partition. The hydrogen peroxide dispersion unit 12 includes a gas distribution network, a storage tank, a delivery pump, and a flash evaporator. The storage tank, delivery pump, and flash evaporator are connected in series through PTFE anti-corrosion pipelines. The output end of the flash evaporator is connected to the gas inlet on the side wall of the sealed box 11 and connected to the gas distribution network. The signal lines of the delivery pump and the flash evaporator are connected to the calculation and evaluation module 4. On this basis, multiple partitions are detachably fixed to the inner wall of the sealed box 11 and cross-separated to form independent placement cavities. This setup allows for the disassembly and adjustment of the partitions according to the instrument dimensions before sterilization, creating independent isolation chambers to prevent material stacking and obstruction. Then, the calculation and evaluation module 4 issues a command to start the delivery pump to extract the agent and send it to the flash evaporator for high-temperature flash evaporation. The generated nano-vapor is distributed throughout all isolation chambers of the sealed box 11 via the gas distribution network. The induction array unit 15 monitors the hydrogen peroxide concentration in real time, thereby solving the problems of uneven steam distribution, sterilization dead corners caused by stacked instruments, and the difficulty of hydrogen peroxide atomized particles penetrating narrow pipes in traditional sterilization boxes, thus improving the uniformity and penetration of steam coverage.

[0046] Optionally, the sealed box 11 also includes a built-in catalytic degradation box and an exhaust valve. The catalytic degradation box is installed flush against the inner wall of the sealed box 11. There are two exhaust valves, both of which are communicatively connected to the computational evaluation module 4. One exhaust valve is installed in the air outlet on the side wall of the sealed box 11 and communicates with the interior of the catalytic degradation box. The other exhaust valve is installed in the air inlet of the catalytic degradation box away from the inner wall of the sealed box 11. The interior of the catalytic degradation box is filled with nano-manganese dioxide catalytic material. The computational evaluation module 4 is also used for:

[0047] After the hydrogen peroxide emission unit 12, the ultraviolet light emission unit 14 and the plasma generation unit 13 are turned off, and before the drive opening and closing connection module 3 is turned on, the exhaust valve is opened. When the hydrogen peroxide concentration is lower than the preset concentration, the exhaust valve is closed.

[0048] Specifically, the catalytic degradation box is a rectangular honeycomb ceramic carrier box filled with 2-5mm nano manganese dioxide catalytic particles, which are snap-fitted to the inner wall of the rear side of the sealed box 11; the exhaust valve is a corrosion-resistant electric fluororubber gate valve with a response time ≤0.5s and pressure feedback sensing function.

[0049] In this optional embodiment, the sealed box 11 also incorporates a catalytic degradation box and an exhaust valve. The exhaust valve at the outlet of the sealed box 11 and the exhaust valve at the inlet of the catalytic degradation box are both connected to the calculation and evaluation module 4 via control lines. The catalytic degradation box is connected to the box's exhaust channel, forming a closed-loop circulating gas path within the box. Thus, after all three-way synergistic sterilization processes are closed, the calculation and evaluation module 4 simultaneously opens both sets of exhaust valves. The mixed gas inside the box circulates through the catalytic degradation box, where the catalytic material continuously decomposes residual hydrogen peroxide and ozone. The sensing array unit 15 provides real-time feedback on the hydrogen peroxide concentration. Once the concentration drops to a preset safety threshold, the module automatically closes the dual exhaust valves, terminating the cyclic degradation. This configuration eliminates the need for external ventilation ducts, achieving closed-loop treatment within the box without external exhaust. This solves the problems of traditional sterilization equipment where residual hydrogen peroxide and ozone concentrations exceed standards after sterilization, leading to prolonged ventilation waiting times and the risk of personnel irritation.

[0050] Optionally, the plasma generating unit 13 includes a plasma electrode, a water-cooled heat dissipation assembly, and a dual-stage radio frequency plasma generator. An embedding groove is provided on the inner wall of the sealed box 11, and the plasma electrode is embedded in the embedding groove. The water-cooled heat dissipation assembly and the dual-stage radio frequency plasma generator are both located outside the sealed box 11 and are both communicatively connected to the calculation and evaluation module 4. The dual-stage radio frequency plasma generator is connected to the water-cooled heat dissipation assembly and electrically connected to the plasma electrode.

[0051] Specifically, the plasma electrodes are titanium alloy array electrodes, with a single electrode length of 150mm, evenly distributed in the slots on the left and right sides of the enclosure; the RF generator has a rated frequency of 13.56MHz, a low power range of 100-150W, and a high pulse power range of 300-500W, and is equipped with a circulating water chiller as a heat dissipation component.

[0052] In this optional embodiment, the plasma generating unit 13 includes a plasma electrode, a water-cooled heat dissipation assembly, and a dual-level radio frequency plasma generator. An embedding groove is provided on the inner wall of the sealed box 11. The dual-level radio frequency plasma generator is connected to the plasma electrode and the water-cooled heat dissipation assembly via pipes / circuits. The control circuit of the dual-level radio frequency plasma generator is connected to the calculation and evaluation module 4. The electrode is embedded and fixed in the embedding groove on the inner wall of the sealed box 11. With this configuration, during the pre-activation process of sterilization, the calculation and evaluation module 4 controls the dual-level radio frequency plasma generator to switch to low-power output, and the electrode continuously discharges to ionize water vapor in the box to generate primary active particles. After hydrogen peroxide vapor is introduced and its concentration reaches the standard, the dual-level radio frequency plasma generator automatically switches to high-pulse power discharge, generating OH and O2 from a large amount of pyrolysis reagent. -Free radicals; based on this, the water-cooled heat dissipation component continuously circulates and cools the environment, keeping the electrode and chamber temperature stably below 50°C. This setup enables precise, staged plasma control, solving the problems of weak pre-activation effect of single-power plasma and chamber overheating caused by prolonged high-power operation, which can damage polymers and thermally sensitive precision instruments.

[0053] Optionally, the ultraviolet light emitting unit 14 includes a power supply element, an integrated fixed lamp assembly, and a protective cover. The inner wall of the sealed box 11 is hinged with a mounting base. The integrated fixed lamp assembly is mounted on the mounting base. The protective cover covers the integrated fixed lamp assembly. The integrated fixed lamp assembly includes 254nm UVC mercury lamps and 275nm deep ultraviolet LEDs arranged at intervals. The power supply element is located outside the sealed box 11 and is electrically connected to the integrated fixed lamp assembly and the computational evaluation module 4, respectively.

[0054] Specifically, the hinge angle of the mounting base can be adjusted from 0 to 180°, and the ultraviolet irradiation angle can be adjusted according to the placement of the partition; the integrated lamp group has two light sources arranged alternately, the outer layer is a high-transparency quartz protective cover, and the reflective plate is made of aluminum high-reflectivity layer.

[0055] In this optional embodiment, the ultraviolet light emitting unit 14 includes a power supply element, an integrated fixed lamp assembly, and a protective cover. A mounting base is hinged to the inner wall of the sealed box 11. The external power supply element is electrically connected to both the integrated fixed lamp assembly and the computational evaluation module 4. The integrated lamp assembly is hinged to the base on the inner wall of the box, and the protective cover completely covers the light source. Thus, during the ternary synergistic sterilization stage, the computational evaluation module 4 controls the power supply element to simultaneously illuminate a 254nm mercury lamp and a 275nm deep ultraviolet LED. The 275nm deep ultraviolet light targets and destroys microbial DNA, while the 254nm ultraviolet light photolyzes ozone byproducts generated by the plasma. The base angle can be manually adjusted in advance to accommodate the adapter placement area. This dual-band synergistic ultraviolet setting solves the problems of single ultraviolet light sources only being able to sterilize or only remove ozone, and the inability to simultaneously address both, while single-band ultraviolet light sources have sterilization dead zones and excessive ozone accumulation.

[0056] Optionally, the ultraviolet light emitting unit 14 also includes a photocatalytic substrate. There are multiple photocatalytic substrates, which are spaced apart on the inner wall of the sealed box 11 and are all located within the light irradiation range of the integrated fixed lamp group. The photocatalytic substrate has a porous honeycomb structure and is covered with a composite photocatalytic functional coating. The composite photocatalytic functional coating is formed by upconversion nanoparticles and graphene quantum dots through low-temperature sintering and uniform loading on the inner and outer pore surfaces of the porous honeycomb structure.

[0057] Specifically, the single photocatalytic substrate is 200mm×100mm in size, with a cordierite honeycomb matrix and a honeycomb pore size of 2mm. It is snap-fitted into the partition or pre-reserved slot in the box wall. The composite coating adopts a low-temperature sintering process of 180℃ and has a coating thickness of 5-10μm.

[0058] In this optional embodiment, the ultraviolet light emitting unit 14 further includes a photocatalytic liner. The photocatalytic liner has no electrical connection wires and relies on spatial ultraviolet light, hydrogen peroxide vapor, and plasma active materials to complete the coupling reaction, forming a spatial synergistic effect with the ultraviolet light emitting unit 14, the hydrogen peroxide dispersing unit 12, and the plasma generating unit 13. With this configuration, during the ternary synchronous sterilization stage, the liner is continuously irradiated with dual-band ultraviolet light, exciting the coating to generate a large number of active free radicals. These free radicals then undergo a chain oxidation reaction with hydrogen peroxide and plasma products within the chamber, superimposing a fourth sterilization effect. Simultaneously, it assists in the decomposition of residual ozone, thereby compensating for the shortcomings of simple physical superposition of plasma, ultraviolet light, and hydrogen peroxide without photocatalytic coupling enhancement. This significantly improves the sterilization guarantee level and shortens the total synergistic sterilization time.

[0059] Optionally, the sensing array unit 15 includes an integrated base and a vacuum sensor, a temperature sensor, a hydrogen peroxide concentration sensor, a dual-band ultraviolet radiation sensor, and an active particle density sensor, all mounted on the integrated base. There are multiple integrated bases, which are evenly distributed on the inner wall of the sealed box 11 and are all communicatively connected to the computation and evaluation module 4.

[0060] Specifically, the integrated base is a polytetrafluoroethylene and 316L composite sealed base, and a single base integrates a five-channel sensor signal conditioning board to output digital signals in a unified manner; the active particle density sensor adopts a miniature Langmuir probe.

[0061] In this optional embodiment, the induction array unit 15 includes an integrated base and vacuum sensors, temperature sensors, hydrogen peroxide concentration sensors, dual-band ultraviolet radiation sensors, and active particle density sensors, all mounted on the integrated base. All integrated bases are uniformly connected to the computational evaluation module 4 via an RS485 bus. The bases are embedded and sealed in the top, side walls, isolation chamber, and bacterial substrate placement area of ​​the chamber, enabling multi-point synchronous data acquisition. Thus, throughout the sterilization process, the five types of sensors continuously and synchronously acquire data on vacuum, temperature, hydrogen peroxide, ultraviolet intensity, and plasma particle density, transmitting this data in real-time to the computational evaluation module 4 for storage and archiving. This data serves as the raw parameters for calculating synergistic gain, thereby overcoming the shortcomings of traditional single-point sensor data—such as incomplete data, inability to identify weak areas in the chamber's sterilization process, and inability to quantify the spatial matching degree of the ternary medium—providing comprehensive raw data support for quantitative analysis of synergistic performance.

[0062] Optionally, the detection module 2 also includes a fluorescence signal acquisition unit 22 and a fluorescence spot imaging unit 23 built into the fluorescence detection box 21, both of which are communicatively connected to the calculation and evaluation module 4. When the mobile base moves into the fluorescence detection box 21, the fluorescence signal acquisition unit 22 and the fluorescence spot imaging unit 23 are both set towards the mobile base. The calculation and evaluation module 4 is also used to: immerse the standard spore-forming sheet on the mobile base by spraying fluorescence buffer solution through the fluorescence signal acquisition unit 22, and after standing for a preset activation time, continuously acquire the characteristic fluorescence signal on the standard spore-forming sheet; synchronously capture the full-area fluorescence spot distribution image of the standard spore-forming sheet through the fluorescence spot imaging unit 23; obtain the real-time fluorescence intensity of the fluorescence spot based on the characteristic fluorescence signal and the full-area fluorescence spot distribution image; retrieve the pre-stored calibration curve of live bacteria and fluorescence intensity fitting; obtain the number of residual live bacteria based on the real-time fluorescence intensity; and combine it with the pre-input initial number of live bacteria on the standard spore-forming sheet to form live bacteria data.

[0063] Specifically, the fluorescence signal acquisition unit 22 is equipped with a 405nm excitation LED array and a silicon photomultiplier tube, which can accurately capture trace amounts of live bacterial fluorescence; the fluorescence point imaging unit 23 adopts a miniature high-definition CMOS camera, which is installed in a light-shielding and sealed manner to prevent external stray light from interfering with the detection values.

[0064] In this optional embodiment, such as Figure 1 As shown, the detection module 2 also includes a built-in fluorescence signal acquisition unit 22 and a fluorescence spot imaging unit 23. The fluorescence signal acquisition unit 22 and the fluorescence spot imaging unit 23 communicate with the computational evaluation module 4. After the mobile base carrying the bacterial slide completely enters the fluorescence detection box 21, the module issues a detection command. The fluorescence signal acquisition unit 22 automatically sprays sterile fluorescence buffer to infiltrate the spores. After a preset activation time, it acquires the fluorescence intensity, and the fluorescence spot imaging unit 23 simultaneously captures a full-area fluorescence image, which is then transmitted to the computational evaluation module 4. In this way, the computational evaluation module 4 can retrieve the factory calibration curve and, combined with the pre-stored initial viable bacteria count, calculate the residual viable bacteria count after sterilization, generating basic viable bacteria data. This replaces the traditional offline bacterial culture and counting method, eliminating the need for manual operation and solving the problems of long offline detection times, data lag, easy introduction of exogenous bacterial contamination, and inability to synchronously match sterilization process parameters.

[0065] Optionally, the detection module 2 further includes a process data storage unit 24, which contains theoretical data on the sterilization process and is communicatively connected to the calculation and evaluation module 4. The calculation and evaluation module 4 is also used for: obtaining the sterility assurance level value of the standard spore tablets based on the live bacteria data; when the sterility assurance level value is greater than a preset threshold, the sterilization process is deemed to have met the standard; retrieving all data collected by the induction array unit 15 during the sterilization process, removing invalid data and aligning it according to the time axis to form a synchronous time-series curve dataset with time corresponding to hydrogen peroxide concentration, discharge power, and irradiation intensity; based on the synchronous time-series curve dataset, retrieving multiple sets of theoretical sterility assurance level values ​​for hydrogen peroxide, plasma, and ultraviolet light under the same sterilization conditions through the process data storage unit 24, taking the maximum value of the multiple sets of theoretical sterility assurance level values ​​as a comparison benchmark, and dividing the difference between the sterility assurance level value and the comparison benchmark by the comparison benchmark as the quotient of the synergistic sterilization gain coefficient; when the synergistic sterilization gain coefficient is greater than zero, determining that the synergistic sterilization effect is better than the sterilization effect of the single sterilization medium, and generating a sterilization evaluation result.

[0066] Specifically, the process data storage unit 24 is a local industrial solid-state drive, which is partitioned to store single sterilization control process libraries for hydrogen peroxide, plasma, and ultraviolet light.

[0067] In this optional embodiment, such as Figure 1 As shown, the detection module 2 is also equipped with a process data storage unit 24, which contains theoretical sterilization process data. The process data storage unit 24 interacts bidirectionally with the calculation and evaluation module 4. The calculation and evaluation module 4 can retrieve internal single-strain sterilization theoretical aseptic assurance level data at any time. The calculation and evaluation module 4 first calculates the current ternary synergistic aseptic assurance level value based on the live bacteria data and compares it with a preset qualified threshold to determine whether sterilization meets the standard. Then, it retrieves the full-process time-series data from the induction array unit 15 and standardizes and aligns it. It retrieves three sets of single-strain sterilization aseptic assurance level values ​​from the storage unit, takes the maximum value as the comparison benchmark, and automatically calculates the synergistic gain coefficient. If the gain coefficient > 0, it is determined that there is a coupling synergistic effect. All data are integrated to generate a complete sterilization evaluation report. This enables digital and quantitative determination of the ternary synergistic effect, unlike existing equipment which can only output whether sterilization is qualified but cannot quantify the magnitude of the synergistic effect of the three components or locate parameter matching defects. This provides a standardized and quantitative basis for process iteration and equipment quality control.

[0068] Secondly, an embodiment of the present invention provides a method for evaluating the synergistic sterilization of hydrogen peroxide plasma and ultraviolet light, comprising: S1, after placing the material to be sterilized and standard spore tablets in the sealed box 11 of the sterilization module 1 of the synergistic sterilization evaluation system of hydrogen peroxide plasma and ultraviolet light, sealing the sealed box 11, and driving the plasma generating unit 13 of the sterilization module 1 to construct a primary active environment by discharging at a preset low power; S2, driving the hydrogen peroxide dissipation unit 12 of the sterilization module 1 to output nano-vapors, collecting the hydrogen peroxide concentration through the sensing array unit 15 of the sterilization module 1, and driving the ultraviolet light of the sterilization module 1 when the hydrogen peroxide concentration reaches a preset concentration, driving the ultraviolet light of the sterilization module 1 to discharge. When the external light emission unit 14 is turned on, the plasma generation unit 13 of the sterilization module 1 is synchronously adjusted to a preset high power. In step S3, the hydrogen peroxide emission unit 12, the ultraviolet light emission unit 14, and the plasma generation unit 13 are driven to shut down after running synchronously for a preset sterilization time. The communication module 3 of the hydrogen peroxide plasma and ultraviolet synergistic sterilization evaluation system is turned on. At the same time, the movable base in the sealed box 11 is driven to move into the fluorescence detection box 21 of the detection module 2 of the hydrogen peroxide plasma and ultraviolet synergistic sterilization evaluation system through the opening and closing communication module 3. The viable bacteria data of the standard spore strips are detected through the fluorescence detection box 21, and the sterilization evaluation result is obtained by calculation.

[0069] like Figure 2 As shown, the technical effect of the hydrogen peroxide plasma and ultraviolet synergistic sterilization evaluation method in this embodiment is similar to that of the hydrogen peroxide plasma and ultraviolet synergistic sterilization evaluation system described above, and will not be repeated here.

[0070] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A hydrogen peroxide plasma and ultraviolet light synergistic sterilization evaluation system, characterized in that, It includes a sterilization module (1), a detection module (2), an opening and closing communication module (3), and a calculation and evaluation module (4); the sterilization module (1) includes a sealed box (11) and a hydrogen peroxide dissipation unit (12), a plasma generation unit (13), an ultraviolet light emission unit (14), and a sensing array unit (15) built into the sealed box (11); the detection module (2) includes a fluorescence detection box (21); the sealed box (11) and the fluorescence detection box (21) are connected through the opening and closing communication module (3) so that the moving base of the sealed box (11) carrying the standard spore strip can be moved into the detection box (21) or returned to the sealed box (11). The computational evaluation module (4) is communicatively connected to the hydrogen peroxide dissipation unit (12), the plasma generation unit (13), the ultraviolet light emission unit (14), the induction array unit (15), the fluorescence detection box (21), the opening and closing communication module (3), and the mobile base, and is used for: After the material to be sterilized and the standard spore tablets are placed in the sealed box (11), the sealed box (11) is closed, and the plasma generating unit (13) is driven to construct a primary active environment with a preset low-power discharge; The hydrogen peroxide dispersion unit (12) is driven to output nano vapor, and the hydrogen peroxide concentration is collected by the induction array unit (15). When the hydrogen peroxide concentration reaches the preset concentration, the ultraviolet light emission unit (14) is driven to turn on, and the plasma generation unit (13) is synchronously adjusted to the preset high power. The hydrogen peroxide emitting unit (12), the ultraviolet light emitting unit (14) and the plasma generating unit (13) are driven to shut down after running synchronously for a preset sterilization time. The opening and closing communication module (3) is driven to open. At the same time, the moving base is driven to move into the fluorescence detection box (21) through the opening and closing communication module (3). The live bacteria data of the standard spore strip are detected through the fluorescence detection box (21), and the sterilization evaluation result is obtained by calculation.

2. The hydrogen peroxide plasma and ultraviolet synergistic sterilization evaluation system as described in claim 1, characterized in that, The sealed box (11) also has a built-in partition. The hydrogen peroxide dispersing unit (12) includes a gas distribution network, a storage tank, a delivery pump and a flash evaporator. The edge of the partition is detachably connected to the inner wall of the sealed box (11) and there are multiple partitions. The multiple partitions are arranged crosswise to separate multiple isolation chambers in the sealed box (11) for the classified placement of the materials to be sterilized. The gas distribution network is evenly distributed on the inner wall of the sealed box (11) and multiple air outlets are evenly distributed on the pipe wall. The storage tank, delivery pump and flash evaporator are connected in sequence and are all located outside the sealed box (11). The flash evaporator is connected to the air inlet of the gas distribution network located on the side wall of the sealed box (11) and is communicatively connected to the calculation and evaluation module (4) along with the delivery pump.

3. The hydrogen peroxide plasma and ultraviolet synergistic sterilization evaluation system as described in claim 2, characterized in that, The sealed box (11) also has a built-in catalytic degradation box and an exhaust valve. The catalytic degradation box is installed against the inner wall of the sealed box (11). There are two exhaust valves, both of which are communicatively connected to the calculation and evaluation module (4). One exhaust valve is installed in the air outlet on the side wall of the sealed box (11) and communicates with the inside of the catalytic degradation box. The other exhaust valve is installed in the air inlet of the catalytic degradation box away from the inner wall of the sealed box (11). The catalytic degradation box is filled with nano-manganese dioxide catalytic material. The calculation and evaluation module (4) is also used for: After the hydrogen peroxide emitting unit (12), the ultraviolet light emitting unit (14) and the plasma generating unit (13) are shut down, and before the opening and closing communication module (3) is opened, the exhaust valve is opened. When the hydrogen peroxide concentration is lower than the preset concentration, the exhaust valve is closed.

4. The hydrogen peroxide plasma and ultraviolet synergistic sterilization evaluation system as described in claim 2, characterized in that, The plasma generating unit (13) includes a plasma electrode, a water-cooled heat dissipation assembly, and a dual-stage radio frequency plasma generator. The inner wall of the sealed box (11) is provided with an embedding groove, and the plasma electrode is embedded in the embedding groove. The water-cooled heat dissipation assembly and the dual-stage radio frequency plasma generator are both located outside the sealed box (11) and are both communicatively connected to the calculation evaluation module (4). The dual-stage radio frequency plasma generator is connected to the water-cooled heat dissipation assembly and electrically connected to the plasma electrode.

5. The hydrogen peroxide plasma and ultraviolet light synergistic sterilization evaluation system as described in claim 2, characterized in that, The ultraviolet light emitting unit (14) includes a power supply element, an integrated fixed lamp group and a protective cover. The inner wall of the sealed box (11) is hinged with a mounting base. The integrated fixed lamp group is mounted on the mounting base. The protective cover covers the integrated fixed lamp group. The integrated fixed lamp group includes 254nm UVC mercury lamps and 275nm deep ultraviolet LEDs arranged at intervals. The power supply element is located outside the sealed box (11) and is electrically connected to the integrated fixed lamp group and the calculation evaluation module (4) respectively.

6. The hydrogen peroxide plasma and ultraviolet synergistic sterilization evaluation system as described in claim 5, characterized in that, The ultraviolet light emitting unit (14) also includes a photocatalytic liner. There are multiple photocatalytic liners, which are spaced apart on the inner wall of the sealed box (11) and are all located within the light irradiation range of the integrated fixed lamp group. The photocatalytic liner has a porous honeycomb structure and is covered with a composite photocatalytic functional coating. The composite photocatalytic functional coating is formed by upconversion nanoparticles and graphene quantum dots through low-temperature sintering and uniformly loading on the inner and outer pore surfaces of the porous honeycomb structure.

7. The hydrogen peroxide plasma and ultraviolet synergistic sterilization evaluation system as described in any one of claims 1 to 6, characterized in that, The sensing array unit (15) includes an integrated base and a vacuum sensor, a temperature sensor, a hydrogen peroxide concentration sensor, a dual-band ultraviolet radiation sensor and an active particle density sensor, all mounted on the integrated base. There are multiple integrated bases, which are evenly distributed on the inner wall of the sealed box (11) and are all communicatively connected to the calculation and evaluation module (4).

8. The hydrogen peroxide plasma and ultraviolet synergistic sterilization evaluation system as described in any one of claims 1 to 6, characterized in that, The detection module (2) further includes a fluorescence signal acquisition unit (22) and a fluorescence spot imaging unit (23) built into the fluorescence detection box (21), both of which are communicatively connected to the calculation and evaluation module (4). When the movable base moves into the fluorescence detection box (21), the fluorescence signal acquisition unit (22) and the fluorescence spot imaging unit (23) are both oriented towards the movable base. The calculation and evaluation module (4) is also used for: The standard spore-forming tablet on the mobile base is soaked in fluorescent buffer solution by the fluorescent signal acquisition unit (22), and after being left to stand for a preset activation time, the characteristic fluorescent signals on the standard spore-forming tablet are continuously acquired. The fluorescence dot imaging unit (23) synchronously captures the full-area fluorescence dot distribution image of the standard spore-forming sheet. Based on the characteristic fluorescence signal and the full-area fluorescence dot distribution image, the real-time fluorescence intensity of the fluorescence dot is obtained. The pre-stored calibration curve of live bacteria and fluorescence intensity is retrieved. Based on the real-time fluorescence intensity, the number of residual live bacteria is obtained and combined with the pre-input initial number of live bacteria of the standard spore-forming sheet to form the live bacteria data.

9. The hydrogen peroxide plasma and ultraviolet synergistic sterilization evaluation system as described in claim 8, characterized in that, The detection module (2) further includes a process data storage unit (24), which contains theoretical data on the sterilization process and is communicatively connected to the calculation and evaluation module (4). The calculation and evaluation module (4) is also used for: The sterility assurance level value of the standard spore tablet is obtained based on the live bacteria data. When the sterility assurance level value is greater than a preset threshold, the sterilization process is deemed to have met the standard. All data collected by the induction array unit (15) during the sterilization process are retrieved, invalid data are removed and aligned with the time axis to form a synchronous time-series curve dataset with time as a function of hydrogen peroxide concentration, discharge power and irradiation intensity respectively; Based on the synchronous time-series curve dataset, the process data storage unit (24) retrieves multiple sets of theoretical sterility guarantee levels for single sterilization media under the same sterilization conditions for hydrogen peroxide, plasma, and ultraviolet light. The maximum value of the multiple sets of theoretical sterility guarantee levels is taken as a comparison benchmark. The quotient obtained by dividing the difference between the sterility guarantee level value and the comparison benchmark by the comparison benchmark is taken as the synergistic sterilization gain coefficient. When the synergistic sterilization gain coefficient is greater than zero, it is determined that the synergistic sterilization effect is better than the sterilization effect of a single sterilization medium, and the sterilization evaluation result is generated.

10. A method for evaluating the synergistic sterilization of hydrogen peroxide plasma and ultraviolet light, characterized in that, include: S1. After the materials to be sterilized and the standard spore tablets are placed in the sealed box (11) of the sterilization module (1) of the hydrogen peroxide plasma and ultraviolet synergistic sterilization evaluation system, the sealed box (11) is closed, and the plasma generation unit (13) of the sterilization module (1) is driven to construct a primary active environment with preset low power discharge. S2. Drive the hydrogen peroxide emitting unit (12) of the sterilization module (1) to output nano vapor, collect the hydrogen peroxide concentration through the sensing array unit (15) of the sterilization module (1), and when the hydrogen peroxide concentration reaches the preset concentration, drive the ultraviolet light emitting unit (14) of the sterilization module (1) to turn on, and synchronously adjust the plasma generating unit (13) of the sterilization module (1) to the preset high power. S3. Drive the hydrogen peroxide emitting unit (12), the ultraviolet light emitting unit (14) and the plasma generating unit (13) to shut down after synchronous operation for a preset sterilization time, drive the communication module (3) of the hydrogen peroxide plasma and ultraviolet synergistic sterilization evaluation system to open, and drive the movable base in the sealed box (11) to move into the fluorescence detection box (21) of the detection module (2) of the hydrogen peroxide plasma and ultraviolet synergistic sterilization evaluation system through the opening and closing communication module (3), and detect the viable bacteria data of the standard spore strip through the fluorescence detection box (21) to calculate and obtain the sterilization evaluation result.

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