Composite carbon source for biochemical treatment and preparation method thereof

Abstract

The application discloses a kind of composite carbon source for biochemical treatment and its preparation method, and composite carbon source is made of 30-50% quick-acting carbon source, 20-40% slow-release carbon source, 0.1-1% active additive and 10-49.9% stabilizer carrier according to mass percentage, and its preparation method is: first, pre-hydrolysis corn syrup dry powder and cellulose derivative are mixed to obtain slow-release carbon source mixture, then part of deionized water in stabilizer carrier and enzyme preparation in active additive are mixed, and enzymolysis reaction is carried out under the condition of 60-80 DEG C, pH 6.5-7.5 to form first intermediate liquid, sodium acetate, glucose and low molecular alcohol vitamin and trace element salt are dissolved in the remaining deionized water and glycerol to form second intermediate liquid;First intermediate liquid is mixed with second intermediate liquid to obtain composite carbon source product, and the application improves the absorption and conversion efficiency of microorganism to carbon source, reduces carbon source dosage and total processing cost.

Description

Technical Field

[0001] This invention relates to the fields of environmental engineering and biotechnology, and in particular to a composite carbon source for biochemical treatment and its preparation method. Background Technology

[0002] Existing biochemical treatment processes using external carbon sources suffer from several problems, which limit carbon source utilization efficiency and treatment stability:

[0003] 1. Limited availability and high cost: Existing fast-acting carbon sources (such as single acetate or methanol) often have uneven utilization rates. A single substance is difficult to provide the best immediate electron donor under different biochemical conditions, and may lead to high costs or the risk of bioinhibition.

[0004] 2. Insufficient long-term effectiveness and unstable supply: Traditional carbon sources (including fast-acting and some simple slow-release materials) cannot provide a sustained and stable carbon supply during deep treatment and long-term low-load phases. They are prone to carbon source depletion, leading to fluctuations in treatment efficiency. Moreover, using fast-acting carbon sources for long-term supply is extremely uneconomical.

[0005] 3. Low bioavailability and lack of synergistic mechanisms: Due to the lack of biosynergistic and catalytic mechanisms, microorganisms have low efficiency in degrading and utilizing macromolecular carbon sources, resulting in carbon source waste, and the reaction rate is limited by the endogenous activity of the microorganisms themselves.

[0006] 4. Poor physicochemical stability: Simple carbon source mixtures lack stability and are prone to precipitation, stratification, or microbial contamination, resulting in short storage periods, inconvenient transportation, and difficulty in controlling metering accuracy during actual addition. Summary of the Invention

[0007] To overcome the above-mentioned defects, the present invention provides a composite carbon source for biochemical treatment and its preparation method. The composite carbon source for biochemical treatment can ensure the stability and long-term effectiveness of biochemical reactions, improve the absorption and conversion efficiency of carbon sources by microorganisms, and reduce the amount of carbon source added and the total treatment cost.

[0008] The technical solution adopted by this invention to solve its technical problem is as follows: a composite carbon source for biochemical treatment, composed of the following components by mass percentage: 30-50% fast-acting carbon source, 20-40% slow-release carbon source, 0.1-1% active adjuvant, and 10-49.9% stabilizer carrier. During the biochemical reaction, the fast-acting carbon source provides the necessary carbon during the rapid degradation phase by microorganisms, while the slow-release carbon source provides a long-lasting and stable carbon source through slow release, ensuring sufficient carbon source participation even in the later stages of the reaction. The trace amounts of active adjuvant effectively enhance microbial activity, achieving a synergistic effect and improving carbon source conversion rate. The stabilizer carrier improves the physical properties of the carbon source, making it less prone to precipitation and stratification, allowing it to fully participate in the reaction and improving the stability of the carbon source.

[0009] As a further improvement of the present invention, the readily available carbon source is composed of the following components by mass percentage: 50-80% sodium acetate, 10-30% glucose, and 5-20% low-molecular-weight alcohols. These components form a multi-component mixed readily available carbon source, wherein sodium acetate is primarily used for denitrification, while glucose and low-molecular-weight alcohols supplement electron donors for different metabolic pathways, achieving rapid synergistic effects. This readily available carbon source can provide optimal immediate electron donors under various biochemical conditions, avoiding the risk of bioinhibition and ensuring rapid degradation of wastewater under various biochemical conditions. The interaction of various components effectively balances the degradation rate and reduces costs.

[0010] As a further improvement of the present invention, the low molecular weight alcohol is ethanol or propanol.

[0011] As a further improvement of the present invention, the slow-release carbon source is composed of the following components in weight percentage: 60-90% pre-hydrolyzed corn steep liquor powder and 10-40% cellulose derivatives. This combined slow-release carbon source can provide a sustained and stable carbon supply during deep treatment and long-term low-load phases, avoiding carbon source depletion and ensuring stable biochemical treatment efficiency. This slow-release carbon source has significant advantages of high efficiency, low cost, and continuous carbon supply.

[0012] As a further improvement of the present invention, the cellulose derivative is sodium hydroxymethylcellulose, hydroxypropylcellulose, hydroxypropyl methylcellulose or hydroxyethylcellulose.

[0013] As a further improvement of the present invention, the active adjuvant is composed of the following components in weight percentage: 50-70% enzyme preparation, 10-20% vitamins, and 20-30% trace element salts. The introduction of the enzyme preparation significantly improves the conversion rate of the slow-release carbon source, forming a biosynergistic and catalytic mechanism, enhancing microbial activity, and facilitating the efficient degradation of macromolecular carbon sources by microorganisms, resulting in high carbon source conversion and utilization efficiency.

[0014] As a further improvement of the present invention, the enzyme preparation is a neutral protease, a highly active complex amylase or a cellulase; the trace element salt is magnesium sulfate or calcium chloride; and the vitamin is a water-soluble vitamin, such as B12, B1, or B6.

[0015] As a further improvement of the present invention, the stabilizer carrier is composed of the following components by mass percentage: 80-100% deionized water and 0-20% glycerol. By using a large amount of deionized water and a small amount of glycerol as a stabilizer / carrier to support the carbon source mixture, the carbon source is suspended in the water, preventing precipitation or stratification, thus ensuring the long-term stability of the carbon source. Furthermore, because the carbon source can fully react, it is easier to control the dosage and improve the accuracy of dosing.

[0016] A method for preparing a composite carbon source for biochemical treatment includes the following steps:

[0017] Step 1: Pretreatment of slow-release components: Weigh the pre-hydrolyzed corn steep liquor powder and cellulose derivative according to the preset ratio, and mix them to obtain a slow-release carbon source mixture. Then, mix the slow-release carbon source mixture with part of the deionized water in the stabilizer carrier and the enzyme preparation in the active agent. Carry out the enzymatic hydrolysis reaction for 2-4 hours at a temperature of 60-80℃ and a pH range of 6.5-7.5 to ensure that the macromolecular carbon source is fully decomposed into small molecules to form the first intermediate liquid.

[0018] Step 2: Dissolving the fast-acting carbon source and auxiliary agents: Weigh out sodium acetate, glucose and low molecular weight alcohols according to the preset ratio of the fast-acting carbon source, and dissolve the vitamins and trace element salts in the fast-acting carbon source and active auxiliary agents in the remaining deionized water and glycerin in the stabilizer carrier to form the second intermediate solution.

[0019] Step 3: Final compounding: The first intermediate liquid (hydrolysate of the slow-release component) and the second intermediate liquid (solution of the fast-acting adjuvant) are thoroughly mixed and stirred evenly to obtain the final compound carbon source product.

[0020] As a further improvement of the present invention, a fourth step is provided: removing insoluble residues from the composite carbon source product by filtration and adjusting the pH to 5-7. The composite carbon source can be produced in a ready-to-use manner, or it can be stored by sterilization or the addition of preservatives.

[0021] The beneficial effects of this invention are as follows: This invention provides carbon through the synergistic supply of fast-acting carbon source and slow-release carbon source. The fast-acting carbon source provides an immediate electron donor to meet the rapid growth requirements of microorganisms, while the slow-release carbon source provides continuous carbon source support, ensuring the stability and long-term effectiveness of the biochemical reaction. By adding trace amounts of active adjuvants (including enzyme preparations), the absorption and conversion efficiency of carbon source by microorganisms is effectively improved, reducing the amount of carbon source added and significantly improving the utilization rate of carbon source. This invention also improves the physical properties and stability of carbon source by adding stabilizer carriers, allowing the carbon source to react fully. Furthermore, by introducing a low-cost slow-release carbon source, the total treatment cost is effectively reduced. Detailed Implementation

[0022] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments in the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0023] Example: A method for preparing a composite carbon source for biochemical treatment, the specific steps of which are as follows:

[0024] Step 1: Pretreatment of slow-release components: Weigh the pre-hydrolyzed corn steep liquor powder and cellulose derivative according to the preset ratio, and mix them to obtain a slow-release carbon source mixture. Then, mix the slow-release carbon source mixture with part of the deionized water in the stabilizer carrier and the enzyme preparation in the active agent. Carry out the enzymatic hydrolysis reaction for 2-4 hours at a temperature of 60-80℃ and a pH range of 6.5-7.5 to ensure that the macromolecular carbon source is fully decomposed into small molecules to form the first intermediate liquid.

[0025] Step 2: Dissolving the fast-acting carbon source and auxiliary agents: Weigh out sodium acetate, glucose and low molecular weight alcohols according to the preset ratio of the fast-acting carbon source, and dissolve the vitamins and trace element salts in the fast-acting carbon source and active auxiliary agents in the remaining deionized water and glycerin in the stabilizer carrier to form the second intermediate solution.

[0026] Step 3: Final compounding: The first intermediate liquid (hydrolysate of the slow-release component) and the second intermediate liquid (solution of the fast-acting adjuvant) are thoroughly mixed and stirred evenly to obtain the final compound carbon source product;

[0027] Step 4: Remove insoluble residues from the composite carbon source product by filtration, adjust the pH to 5-7, and finally preserve it by sterilization or adding preservatives.

[0028] A composite carbon source for biochemical treatment, the specific embodiments of which include the amounts of each component (by mass percentage) are shown in Table 1:

[0029]

[0030] Table 1

[0031] The specific amounts of each component (by mass percentage) in the embodiments of the fast-acting carbon source are shown in Table 2:

[0032]

[0033] Table 2

[0034] The amounts of each component (by mass percentage) in the specific embodiments of the slow-release carbon source are shown in Table 3:

[0035]

[0036] Table 3

[0037] The dosage of each component (by mass percentage) in the specific embodiments of the active additives is shown in Table 4:

[0038]

[0039] Table 4

[0040] The amounts of each component (by mass percentage) in the stabilizer carrier examples are shown in Table 5:

[0041]

[0042] Table 5

[0043] In the above embodiments, Example 3 is the optimal formulation. The composite carbon source of this application and the single carbon source (single sodium acetate) were used to carry out biochemical reactions on wastewater. The application effect data are shown in Table 6:

[0044]

[0045] Table 6

[0046] As can be seen from Table 6, the composite carbon source of the present invention has high utilization rate, low dosage, and high total nitrogen removal rate in wastewater. In the process of treating nitrogen-containing wastewater, the cost of using the composite carbon source of the present invention is far lower than the treatment cost of using a traditional single carbon source.

Claims

1. A composite carbon source for biochemical treatment, characterized in that: It consists of the following components by weight percentage: 30-50% fast-acting carbon source, 20-40% slow-release carbon source, 0.1-1% active additives and 10-49.9% stabilizer carrier.

2. The composite carbon source for biochemical treatment according to claim 1, characterized in that: The fast-acting carbon source consists of the following components by mass percentage: 50-80% sodium acetate, 10-30% glucose, and 5-20% low molecular weight alcohols.

3. The composite carbon source for biochemical treatment according to claim 2, characterized in that: The low molecular weight alcohols are ethanol or propanol.

4. The composite carbon source for biochemical treatment according to claim 1, characterized in that: The slow-release carbon source consists of the following components in weight percentage: 60-90% pre-hydrolyzed corn steep liquor powder and 10-40% cellulose derivatives.

5. The composite carbon source for biochemical treatment according to claim 4, characterized in that: The cellulose derivative is sodium hydroxymethylcellulose, hydroxypropylcellulose, hydroxypropyl methylcellulose, or hydroxyethylcellulose.

6. The composite carbon source for biochemical treatment according to claim 1, characterized in that: The active ingredient is composed of the following components in weight percentage: 50-70% enzyme preparation, 10-20% vitamins and 20-30% trace element salts.

7. The composite carbon source for biochemical treatment according to claim 6, characterized in that: The enzyme preparation is a neutral protease, a highly active complex amylase, or a cellulase; the trace element salt is magnesium sulfate or calcium chloride; and the vitamin is a water-soluble vitamin.

8. The composite carbon source for biochemical treatment according to claim 1, characterized in that: The stabilizer carrier is composed of the following components by mass percentage: 80-100% deionized water and 0-20% glycerol.

9. A method for preparing a composite carbon source for biochemical treatment according to any one of claims 1-9, characterized in that: Includes the following steps: Step 1: Pretreatment of slow-release components: Weigh the pre-hydrolyzed corn steep liquor powder and cellulose derivative according to the preset ratio, and mix them to obtain a slow-release carbon source mixture. Then, mix the slow-release carbon source mixture with part of the deionized water in the stabilizer carrier and the enzyme preparation in the active agent. Carry out the enzymatic hydrolysis reaction for 2-4 hours at a temperature of 60-80℃ and a pH range of 6.5-7.5 to ensure that the macromolecular carbon source is fully decomposed into small molecules to form the first intermediate liquid. Step 2: Dissolving the fast-acting carbon source and auxiliary agents: Weigh out sodium acetate, glucose and low molecular weight alcohols according to the preset ratio of the fast-acting carbon source, and dissolve the vitamins and trace element salts in the fast-acting carbon source and active auxiliary agents in the remaining deionized water and glycerin in the stabilizer carrier to form the second intermediate solution. Step 3: Final compounding: Mix the first intermediate liquid and the second intermediate liquid thoroughly and stir evenly to obtain the final compound carbon source product.

10. The method for preparing a composite carbon source for biochemical treatment according to claim 9, characterized in that: There is also a fourth step: removing insoluble residues from the composite carbon source product by filtration and adjusting the pH to 5-7.

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