A microbial sample processing incubation visual inspection apparatus

Abstract

The present application relates to the field of microbiology device, specifically to a kind of microbial sample processing culture visual inspection equipment, in turn including homogenization unit, inspection processing unit and visual counting unit, homogenization unit includes bearing assembly, strike component, seal and extraction component and shape adjustment component, seal and extraction component includes sliding seat, guide seat and pressure frame, the front end of pressure frame is equipped with several suction cups, shape adjustment component includes contact roller, and homogenization bag is driven to adjust internal volume by contact roller, homogenization bag is made into suspended state by seal and extraction component adsorption homogenization bag and fast lifting, and the air in bag body part is discharged by the contact roller of shape adjustment component, then by the alternate beating of inclined beating plate, let homogenization bag complete two necessary operations under automatic operation, form the beating mixing form of bottomless bearing suspension type and less air, avoid the problems of flow dead zone, stress concentration, excessive air leading to sample easy to splash and adhere to bag wall caused by homogenization bag bottom being carried and transported in existing equipment.

Description

Technical Field

[0001] This invention relates to the field of microbiological devices, specifically a visual inspection device for microbial sample processing and culture. Background Technology

[0002] Microbiological testing in the food industry is a core technical means to ensure food safety. It evaluates the hygienic quality of food and prevents foodborne diseases by identifying the types and quantities of microorganisms in food and its production environment.

[0003] In existing food microbiology testing processes, many important steps have gradually evolved from manual operation to equipment operation, thereby reducing the quality problems caused by the instability of manual speed and accuracy in large-scale testing. However, further automation of operations previously performed manually remains an obstacle. For example, in traditional homogenization, the material in the homogenizing bag is manually diluted with liquid and then manually suspended and clamped for machine mixing. Existing equipment simplifies many steps when trying to achieve the business objective. For instance, the homogenizing bag is supported at the bottom in all operations, making the bends in the bag and the contact area between the bottom and the support surface dead zones. For microbiology testing, this can easily lead to microorganisms on solid samples not being fully washed into the diluent, resulting in significantly low colony counts and false negatives. Summary of the Invention

[0004] To address the problem that "manual operation involves pre-adding samples, injecting liquid, and sealing homogenizing bags before transferring them to the homogenizer for suspension, which is a discrete and interruptible process. In the equipment design, several homogenizing bags are transported with their bottoms supported. Similarly, the homogenizing bags need to be placed in fixed supporting positions before subsequent operations such as opening the bags and injecting liquid can be performed. This results in dead zones at the bottom of the bags, stress concentration at the bottom of the bags when they are hit, and excessive air inside the bags causing samples to splash and adhere to the bag walls, leading to a decrease in the recovery rate of the target substance," this invention provides a visual inspection device for microbial sample processing and culture.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a visual inspection device for microbial sample processing and culture, comprising a homogenization section, an inspection and processing section, and a visual counting section, which are transported between each other via a transfer device. The visual counting section includes a dish carrier for holding culture dishes and a camera for counting the culture dishes. The homogenization section is mounted on a base, on which a high plate and a back plate are fixed. The homogenization section includes a support assembly for holding homogenization bags, a tapping assembly fixed below the high plate, a sealing and extraction assembly fixed above the high plate, a shaping assembly movably mounted on the sealing and extraction assembly, and a pipette for moving and dispensing liquid. The working directions of the sealing and extraction assembly, the shaping assembly, and the tapping assembly all point towards the back plate, and there is a gap between the back plate and the high plate. Furthermore, a placement area is set on the base within the interval; the sealing and lifting assembly includes a sliding base, several guide seats fixed on the sliding base, and a pressing and lifting frame engaged on the guide seats. The front surface of the pressing and lifting frame is provided with several suction cups. The homogenized bag in the placement area is driven by the pressing and lifting frame to perform closing and lifting movements. The pressing and lifting frame drives the homogenized bag to rise on the guide seats for a greater stroke than the downward stroke. A lifting back frame is slidably provided on the back plate to clamp and cooperate with the pressing and lifting frame; the tapping assembly is located below the sealing and lifting assembly. The tapping assembly includes several tapping plates and a drive group that drives the tapping plates to perform alternating movements at intervals. The tapping plates are inclined and the distance between the bottom end and the back plate is smaller than the distance between the top end; the shaping assembly includes a contact roller that performs oscillating movements. The homogenized bag in the placement area is driven by the contact roller to adjust the internal cavity volume.

[0006] Furthermore, the lifting assembly includes a back seat and several guide seats. The back seat and guide seats are fixed on a sliding seat and located on the front and rear sides of the sliding seat. Several rear lifting frames are fixed to the back of the lifting frame, and a holding elastic element is fixed between the back seat and the rear lifting frames. The rear lifting frames are rotatably equipped with wheels on their sides. The guide seats have guide channels for the wheels to engage. The rear lifting frames are movably engaged with the guide seats through the wheels. The rear lifting frames are horizontal on the guide seats, making the front end of the lifting frame vertical. The lifting frame moves horizontally and vertically within the guide channels through the wheels. The angle between the path of the wheels' descent movement and the horizontal plane is greater than the angle between the path of the wheels' ascent movement and the horizontal plane.

[0007] Furthermore, the guide channel includes a base cavity at a low position within the guide seat, an elevation cavity at a high position, and an inclined elevation cavity that connects the base cavity and the elevation cavity. A wall-forming plate is hinged to the inner wall of the side of the guide channel, forming the bottom structural wall of the inclined elevation cavity. A transfer seat is fixed to the inner wall of the side of the guide channel, and a drop opening is provided between the transfer seat and the elevation cavity. A drop cavity for connecting with the base cavity is provided below the drop opening. A relief plate is hinged to the top of the transfer seat, and the relief plate moves within the drop opening and the elevation cavity to connect the inclined elevation cavity and the elevation cavity and to open the drop opening.

[0008] Furthermore, a torsion member is nested between the relief plate and the transfer seat to drive the relief plate to swing upward and open the drop opening. The horizontal height of the bottom of the drop cavity is the same as the horizontal height of the bottom of the base cavity, and a buffer pad is laid at the bottom of the drop cavity.

[0009] Furthermore, there are two fixed positions for the wheels on the rear display frame, with adjacent wheels having different horizontal heights and different distances from the lifting frame.

[0010] Furthermore, the lifting frame includes a follower frame, the front end of which is provided with several suction cups that are staggered with the suction cups of the lifting frame, and the interval between the suction cups is greater than the diameter of the suction cups; a first clearance groove is provided on the back upright plate, and a limiting step is provided at the bottom of the first clearance groove; the lifting frame also includes a back fixing frame located behind the back upright plate and fixedly connected to the follower frame, the fixing frame being slidably connected to the rear end of the back upright plate, and the lifting frame rests against the limiting step by its own weight while retaining the freedom to slide upward.

[0011] Furthermore, the sliding seat has an installation groove, the shaping component is installed in the installation groove on the sliding seat and moves with the sliding seat, the high plate has a second clearance groove, the second clearance groove is fixed with a guide frame to guide the shaping component to swing; the shaping component includes a contact roller, a slide rod, a rotating bushing and a pulling member, the front end of the slide rod is horizontally fixed with a truss, the two ends of the contact roller are rotatably connected to the truss and perpendicular to the slide rod, the sliding seat is fixed with a timing frame, the rotating bushing is rotatably installed on the timing frame, the slide rod is movably nested on the rotating bushing, the end of the slide rod is swayingly connected with a tail deflector arm, the tail deflector arm is fixed with a pull rod, one end of the pulling member is fixed to the pull rod and the other end is fixed to the timing frame, the other side of the pull rod is fixed with a guided member, the guided member contacts the guide frame and thereby drives the tail deflector arm to swing.

[0012] Furthermore, the fixed connection points of the pull member, the pull rod, and the timing frame are located on both sides of the rotating shaft sleeve, respectively. The connection points of the pull member and the pull rod, the connection points of the pull member and the timing frame, and the central axis of the rotating shaft sleeve have three projection points on a vertical plane perpendicular to the central axis. The collinear position of the three projection points is the middle position of the pull member and the pull rod. The pull member provides a pulling force to the pull rod located below the collinear position, causing the slide rod to swing upward. The pull member provides a pulling force to the pull rod located above the collinear position, causing the slide rod to swing downward.

[0013] Furthermore, the guide frame includes an upper lower guide frame with a downward guide slope and an upper lower guide frame with an upward guide slope. The guided component contacts the upper lower guide frame and swings downward as the sliding seat moves forward, and contacts the upper lower guide frame and swings upward as the sliding seat moves backward. The starting position of the upper lower guide frame driving the slide rod to swing upward is earlier than the starting position of the lifting frame rising on the guide seat.

[0014] Furthermore, the driving assembly of the tapping component includes a tapping motor fixed on the base and an eccentric disk fixed on the rotating shaft of the tapping motor. There are two eccentric disks, which are respectively located on both sides of the tapping motor. There are four tapping plates, which are divided into two groups. Each group of tapping plates is fixedly connected to the inner advance seat and the outer advance seat on one side of the tapping motor. The inner advance seat and the outer advance seat are slidably mounted on the base. A connecting rod centering seat is fixed on the base between the inner advance seat, the outer advance seat and the eccentric disk. The top of the connecting rod centering seat is rotatably connected to a second connecting rod and connected to the middle of the second connecting rod. The eccentric disk is connected to one end of the second connecting rod and a first connecting rod. The two ends of the second connecting rod are respectively connected to the inner advance seat and the outer advance seat through a third connecting rod. The bottom end of the tapping plate is provided with a raised section for contacting the bottom of the homogenizing bag.

[0015] Compared with the prior art, the present invention has the following beneficial effects: This invention utilizes a sealing and lifting assembly with a pressure lifting frame and a follow-up lifting frame, guided by a guide seat channel, to adsorb and rapidly elevate the homogenizing bag, changing it from a bottom-supported state to an upper-clamped and suspended state. The bag's volume is adjusted by the contact rollers of the shaping assembly, thus expelling some air from the bag. Then, an inclined tapping plate alternately taps the bag, allowing it to complete two necessary operations automatically. This results in a bottom-supported, suspended, and less air-intensive tapping and mixing process, avoiding the flow dead zones, stress concentrations, and insufficient microbial elution of solid samples caused by the bottom-supported transport of the homogenizing bag in existing equipment, as well as the reduced recovery rate of target substances due to excessive air causing sample splashing and adhesion to the bag wall. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the overall structure of a visual inspection device for microbial sample processing and culture according to the present invention.

[0017] Figure 2 This is a schematic flowchart illustrating the process of microbial processing, culture, and visual inspection in the homogenization unit, inspection processing unit, and visual counting unit of the present invention.

[0018] Figure 3 This is a three-dimensional structural diagram of the homogenizing part of the present invention.

[0019] Figure 4 This is a three-dimensional structural diagram of the carrier component of the present invention.

[0020] Figure 5 This is a three-dimensional structural diagram of the sealing and lifting component, the tapping component, and the homogenizing bag of the present invention.

[0021] Figure 6 This is a side view of the working state of the sealing and lifting component, the tapping component, and the shaping component of the present invention.

[0022] Figure 7This is a three-dimensional structural schematic diagram of the sealing and extraction assembly of the present invention, as well as a side sectional structural schematic diagram of the guide seat and a three-dimensional structural schematic diagram of the guide seat.

[0023] Figure 8 This is a schematic diagram showing the side state changes of the sealing and lifting assembly and the following lifting frame on the back plate of the present invention in cooperating and clamping the homogenized bag, as well as a three-dimensional structural schematic diagram of the following lifting frame and a schematic diagram showing the state changes of the wheels in the guide seat.

[0024] Figure 9 This is a three-dimensional structural diagram of the striking component of the present invention, and a side view of the striking component in two working states.

[0025] Figure 10 This is a three-dimensional structural schematic diagram of the shaping component of the present invention.

[0026] Figure 11 This is a schematic diagram showing the working state changes of the shaping component of the present invention.

[0027] Figure 12 This is a three-dimensional structural diagram of the visual counting unit of the present invention.

[0028] Figure 13 This is a side view of the visual counting unit of the present invention.

[0029] In the diagram: 1. Homogenization section; 2. Inspection and processing section; 3. Visual counting section; 4. Test tube conveyor line; 5. Petri dish conveyor line; 6. Machine base; 7. Homogenization bag; 8. Three-dimensional translation stage; 9. Petri dish; 11. Supporting component; 12. First motor gripper; 13. Pipette; 14. Sealing and lifting component; 15. Tapping component; 16. Shaping component; 17. Liquid dispensing tube; 18. Lifting back support; 19. Suction cup; 41. Dishes tray; 42. Second motor gripper; 43. Second transposition motor; 44. Camera; 45. Fill light; 61. Bracket; 62. Slide rail; 63. Backrest plate; 64. High plate; 65. First clearance groove; 66. Limiting step; 67. Buffer pad; 68. Second clearance groove; 69. Visual support frame; 111. Turntable; 112. Divider plate; 113. First transposition motor; 114. Disc base; 141. Sliding seat; 142. Pressing and lifting frame; 143. Guide seat; 144. Back seat; 145. Translation actuator; 146. Holding elastic element; 147. Wheel; 151. Patting plate; 152. Eccentric disc; 153. Outer advance / retracting seat; 154. Inner advance / retracting seat; 155. First connecting rod; 156. Second connecting rod; 157. Connecting rod centering seat; 158. Third connecting rod; 159. Patting motor; 161. Contact roller; 162. Slide rod; 163. Rotating shaft sleeve; 164. Synchronizing frame; 165. Tail reversing arm; 166. Pull rod; 167. Pulling element; 168. Guided element; 169. Guide frame; 181. Follower frame; 182. Back fixing frame; 1411. Mounting slot; 1421. Rear display frame; 1431. Base cavity; 1432. Inclined lifting cavity; 1433. Lifting cavity; 1434. Dropping cavity; 1435. Transfer seat; 1436. Yielding plate; 1437. Wall panel; 1438. Drop opening; 1691. Upper guide and lower frame; 1692. Lower guide and upper frame. Detailed Implementation

[0030] The technical solution of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are some embodiments of the present invention, but not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0031] Examples, such as Figures 1-13 As shown: This invention provides a visual inspection device for microbial sample processing and culture, comprising a homogenization section 1, an inspection and processing section 2, and a visual counting section 3. The sections are connected by a transfer device. The homogenization section 1 and the inspection and processing section 2 are connected by a test tube transfer line 4, and the inspection and processing section 2 and the visual counting section 3 are connected by a dish transfer line 5. The test tube transfer line 4 and the dish transfer line 5 can be industrial robots or a three-dimensional translation stage 8 combined with motor grippers and a transfer line to form a gripping, placing, and conveying device. The homogenization section 1 is used to dilute and mix the material in the homogenization bag 7, and then transfer the mixture to test tubes. The inspection and processing section 2 uses existing transfer equipment to gradient dilute the mixture in the test tubes and transfer it to the culture dish 9, including but not limited to pour transfer and spread transfer. The visual counting unit 3 is mounted on the base 6, including a visual support 69 fixedly mounted on the base 6. A dish carrier 41 for carrying the culture dish 9 is rotatably mounted on the base 6. A second transposition motor 43 is fixed in the middle of the base 6 and connected to the dish carrier 41, thereby driving the dish carrier 41 to rotate. A camera 44 for counting the culture dish 9 and supplementary lights 45 surrounding the camera 44 are fixed on the visual support 69. A bracket 61 is fixed on the base 6, and a three-dimensional translation stage 8 is fixed on the bracket 61. The three-dimensional translation stage 8 is a three-axis translation mechanism in the prior art, including an X-axis for left and right movement and a forward and backward movement. The Y-axis and the vertically moving Z-axis are used to drive the required end effector to move in three dimensions (XYZ) within space. A second motor gripper 42 is fixed at the bottom of the Z-axis. The second motor gripper 42 is an existing electric gripper product. The gripper mechanism is formed by the gripper and the motor. The motor drives the gripper to open and close through gear transmission, worm gear transmission, etc., thereby clamping the culture dish 9 and placing it on the dish carrier plate 41. The dish carrier plate 41 rotates to send the culture dish 9 to be detected to the area below the camera 44. The camera 44 uses an existing machine vision solution combined with a counting algorithm to calculate the number of bacteria in the culture dish 9.

[0032] The homogenizing unit 1 is mounted on a base 6, which is fixed with a high plate 64 and a back plate 63. The homogenizing unit 1 includes a carrying assembly 11 for carrying homogenizing bags 7, a tapping assembly 15 fixed below the high plate 64, a sealing and lifting assembly 14 fixed above the high plate 64, a shaping assembly 16 movably mounted on the sealing and lifting assembly 14, and a pipette 13 for moving and picking up / discharging liquids. The carrying assembly 11 includes a tray 114 fixed on the base 6, a turntable 111 rotatably mounted on the tray 114, and a first shifting motor 113 fixed below the base 6. The first shifting motor 113 is connected to the turntable 111 to drive the turntable 111 to rotate. The turntable 111 has a plurality of partition plates 112 arranged in a ring on it. The partition plates 112 are used to place the homogenizing bags 7. A feeding window is provided on one side of the base 6 for operators or industrial robots to place the homogenizing bags 7. Between the partition plate 112 of the turntable 111, the working directions of the sealing and lifting assembly 14, the shaping assembly 16, and the striking assembly 15 all point towards the back plate 63. There is a gap between the back plate 63 and the high plate 64, and a placement area is set on the base 6 within the gap. A liquid addition pipe 17 is fixed on the top of the back plate 63. The liquid addition pipe 17 is connected to an external pump body for adding diluent to the homogenization bag 7. A bracket 61 is fixed on the base 6, and a three-dimensional translation stage 8 is fixed on the bracket 61. The three-dimensional translation stage 8 is provided with two Z-axis. A first motor gripper 12 is fixed at the bottom of one Z-axis for transferring the homogenization bag 7 on the turntable 111 to the placement area. A pipette 13 is fixed at the bottom of the other Z-axis. A test tube rack is fixed on the side of the base 6 near the test tube delivery line 4. Test tubes are placed in the test tube rack. The pipette 13 is used to transfer the mixture in the homogenization bag 7 into the test tube.

[0033] The sealing and lifting assembly 14 includes a sliding seat 141, several guide seats 143 fixed on the sliding seat 141, and a lifting frame 142 engaged with the guide seats 143. A translation actuator 145 is fixed at the rear of the sliding seat 141, and the front end of the translation actuator 145 is fixedly connected to the sliding seat 141. The translation actuator 145 can be in the form of a cylinder, electric cylinder, etc., and is used to drive the sliding seat 141 to move. Slide rails 62 are fixed on both sides of the high plate 64, and the two ends of the sliding seat are slidably nested by several sliders. On the slide rail 62, the front end plane of the pressure lifting frame 142 is provided with several suction cups 19. The suction cups 19 are connected to external valves and pump bodies to create negative pressure to adsorb the homogenizing bag 7. The homogenizing bag 7 in the placement area is driven by the pressure lifting frame 142 to perform closing, lifting and lowering movements. The back plate 63 is slidably provided with a lifting back frame 18 to clamp and cooperate with the pressure lifting frame 142. It should be noted that the stroke of the pressure lifting frame 142 driving the homogenizing bag 7 to rise on the guide seat 143 is greater than the stroke of falling. In this regard, the sealing and lifting assembly 14 includes a back seat 144 and several guide seats 143. The back seat 144 and guide seats 143 are fixed on the sliding seat 141 and located on the front and rear sides of the sliding seat 141. In this embodiment, there are four guide seats 143, and the horizontal height of the two outer ones is different from that of the two middle ones. Several rear lifting frames 1421 are fixed on the back of the lifting frame 142. A lifting elastic member 146 is fixed between the back seat 144 and the rear lifting frame 1421. The lifting elastic member 146 is a spring. It should be noted that when the rear lifting frame 1421 moves to a high position by the guide seat 143, the fixing point between the spring and the spring is lower than or equal to the fixing point between the spring and the back seat 144, so that the direction of the force applied by the spring to the rear lifting frame 1421 can make the rear lifting frame 1421 move downward. The rear display frame 1421 is provided with rotatable wheels 147 on its side. The guide seat 143 is provided with a guide channel for the wheels 147 to engage. The rear display frame 1421 is movably engaged with several guide seats 143 by several wheels 147. Specifically, there are two fixed positions of wheels 147 on the rear display frame 1421. Adjacent wheels 147 have different horizontal heights and different distances from the lifting frame 142. The horizontal height of the middle wheel 147 is higher than that of the wheels 147 on both sides, and the middle wheel 147 is closer to the back seat 144. Thus, the outer and inner wheels 147 form a limiting effect on the guide seat 143, so that the rear display frame 1421 is horizontal or tends to be horizontal when moving on several guide seats 143, and the front plane of the lifting frame 142 is vertical. The lifting frame 142 moves horizontally and vertically within the guide channel via wheels 147. The guide channel includes a base cavity 1431 at a lower position within the guide seat 143, an lifting cavity 1433 at a higher position, and an inclined lifting cavity 1432 that connects the base cavity 1431 and the lifting cavity 1433 at an angle. A wall-forming plate 1437 is hinged to the inner wall of the side of the guide channel, forming the bottom structural wall of the inclined lifting cavity 1432. In this embodiment... The inclined cavity 1432 has an inclination angle of 45°. A transfer seat 1435 is fixed on the inner wall of the guide channel. The top of the wall plate 1437 is connected and abuts against the transfer seat 1435. A drop opening 1438 is opened between the transfer seat 1435 and the lifting cavity 1433. Below the drop opening 1438, a positioning cavity 1434 is opened for connection with the base cavity 1431. A relief plate 1436 is hinged to the top of the transfer seat 1435. The relief plate 1436 and A torsion spring is nested between the intermediate transfer seats 1435 to drive the relief plate 1436 to swing upward, thereby opening the drop opening 1438. The relief plate 1436 moves within the drop opening 1438 and the lifting cavity 1433 to connect the inclined lifting cavity 1432 and the lifting cavity 1433, and to open the drop opening 1438. The wheel 147 is stationary within the base cavity 1431, and moves towards the wall plate 1437 during movement, rising through the inclined lifting cavity 1432. At the top of the transfer seat 1435, the relief plate 1436 is squeezed and pressed to transition to the lifting cavity 1433 and remain stationary. At the same time, the torque component drives the relief plate 1436 to swing upward to reset and open the drop opening 1438. When the wheel 147 moves back, it can fall through the drop opening 1438. The horizontal height of the bottom of the drop cavity 1434 is the same as the horizontal height of the bottom of the base cavity 1431. A buffer pad 67 is laid at the bottom of the drop cavity 1434. It should be noted that the angle between the path of the wheel 147's descent and the horizontal plane is greater than the angle between the path of its ascent and the horizontal plane, and the former angle is close to 90°. This ensures that the stroke of the pressure lifting frame 142 driving the homogenizing bag 7 on the guide seat 143 is less than the stroke of its ascent, allowing the homogenizing bag 7 to fall back to the placement area quickly. This avoids the decrease in clamping force after the force direction changes, which would cause the homogenizing bag 7 to fall alternately between the front and rear layers.

[0034] Meanwhile, the lifting frame 18 includes a follower frame 181. The front end plane of the follower frame 181 is provided with a number of suction cups 19, which are staggered with the suction cups 19 of the pressing frame 142. The interval between the suction cups 19 is larger than the diameter of the suction cups 19, so that when the suction cups 19 of the pressing frame 142 are pressed, they point to the interval part of the follower frame 181, forming a better contact surface to facilitate the suction cups 19 to adhere. The back support plate 63 has a first clearance groove 65. The bottom of the first clearance groove 65 has a limiting step 66 for supporting the follow-up back frame 18. The top of the limiting step 66 is covered with a buffer pad 67. The follow-up back frame 18 also includes a back fixing frame 182 located behind the back support plate 63 and fixedly connected to the follower frame 181. The fixing frame is slidably connected to the rear end of the back support plate 63. Specifically, a slide rail 62 is vertically fixed at the rear end of the back support plate 63. The fixing frame is slidably nested on the slide rail 62 by a slider. The follow-up back frame 18 rests against the limiting step 66 by its own weight and retains the freedom to slide upward.

[0035] The shaping component 16 includes a contact roller 161 that swings. The homogenizing bag 7 in the placement area is driven by the contact roller 161 to adjust the internal cavity volume. The sliding seat 141 is provided with a mounting groove 1411, and the high plate 64 is also provided with a second clearance groove 68. The shaping component 16 is installed in the mounting groove 1411 on the sliding seat 141 and moves with the sliding seat 141. A guide frame 169 is fixed on the second clearance groove 68 to guide the shaping component 16 to swing. Therefore, the shaping assembly 16 includes a contact roller 161, a slide rod 162, a rotating bushing 163, and a pulling member 167. The pulling member 167 is a tension spring. The mounting grooves 1411 on both sides of the sliding seat 141 are fixed with a timing frame 164. The rotating bushing 163 is rotatably mounted on the timing frame 164. There are two slide rods 162, which are movably nested on the rotating bushings 163 on both sides. The front end of the slide rod 162 is fixed laterally, and the two slide rods 162 are fixed on the same truss. The two ends of the contact roller 161 rotate. Connected to the truss and perpendicular to the slide bar 162, two contact rollers 161 are provided on the truss with a gap between the two contact rollers 161. The end of the slide bar 162 is oscillatingly connected to the tail deflector arm 165. A pull rod 166 is fixed on the tail deflector arm 165. One end of the pull member 167 is fixed to the pull rod 166 and the other end is fixed to the timing frame 164. A guide member 168 is fixed on the other side of the pull rod 166. The guide member 168 contacts the guide frame 169, thereby driving the tail deflector arm 165 to swing. It should be noted that the fixed connection points of the pull member 167 with the pull rod 166 and the timing frame 164 are located on both sides of the rotating sleeve 163. The connection points of the pull member 167 with the pull rod 166, the connection points of the pull member 167 with the timing frame 164, and the central axis of the rotating sleeve 163 have three projection points on a vertical plane perpendicular to the central axis. The collinear position of the three projection points is the middle position of the pull member 167 and the pull rod 166. The pull member 167 provides a pulling force to the pull rod 166 located below the collinear position to drive the slide rod 162 to swing upward. The pull member 167 provides a pulling force to the pull rod 166 located above the collinear position to drive the slide rod 162 to swing downward. Meanwhile, the guide frame 169 includes an upper lower guide frame 1691 with a downward guide slope and a lower upper guide frame 1692 with an upward guide slope. The guided member 168 contacts the upper lower guide frame 1691 and swings downward as the sliding seat 141 moves forward; the guided member 168 contacts the lower upper guide frame 1692 and swings upward as the sliding seat 141 retracts. The starting position of the upper lower guide frame 1691 driving the slide rod 162 to swing upward is earlier than the starting position of the lifting frame 142 rising on the guide seat 143. When the lifting frame 142 is preparing to rise, it has already pressed the homogenizing bag 7 onto the carrying frame 18. Previously, after the contact roller 161 was driven to swing upward by the slide bar 162, the contact roller 161 rolled over the homogenizing bag 7, which squeezed the space in the upper part of the homogenizing bag 7, thereby reducing some of the air in the homogenizing bag 7. Usually, the air in the homogenizing bag 7 is set to be less than one-third of the bag volume. When it exceeds one-third, the sample is prone to splashing and adhering to the bag wall, resulting in a decrease in the recovery rate of the target substance. Retaining a certain amount of air, such as less than one-third and within the range of 5-10ml, can form an elastic air cushion, so that the impact energy is more evenly distributed to the entire sample, thereby improving the cell membrane rupture efficiency while ensuring the recovery rate of the target substance. As the slide advances further, the contact roller 161 causes the homogenizing bag 7 to press against the back plate 63, while the tail directional arm 165 pulls the puller 167. At the same time, the homogenizing bag 7 rises, causing the contact roller 161 to rotate. The contact roller 161 squeezes the lower part of the homogenizing bag 7, and the air is discharged through the gap between the two contact rollers 161.

[0036] The striking component is located below the sealing and lifting component 14. The striking component includes several striking plates 151 and a drive group that drives the striking plates 151 to move alternately at intervals. The striking plates 151 are inclined and the distance between the bottom end and the back plate 63 is smaller than the distance between the top end. The bottom end of the striking plate 151 is provided with a raised section for contacting the bottom of the raised homogenizing bag 7. To address this, the drive assembly of the striking component includes a striking motor 159 fixed on the base 6 and an eccentric disk 152 fixed on the rotating shaft of the striking motor 159. Two eccentric disks 152 are provided, located on either side of the striking motor 159. Four striking plates 151 are provided, divided into left and right groups. Each group of striking plates 151 is fixedly connected to an inner advance / retractor 154 and an outer advance / retractor 153 on one side of the striking motor 159, respectively. Two parallel slide rails 62 are fixed on each side of the base 6. The inner advance / retractor 154 and the outer advance / retractor 153 are sequentially slidably nested on the slide rails 62 of the base 6 from the inside to the outside of the striking motor 159 via sliders. A connecting rod centering seat is fixed on the base 6 between the inner advance / retractor 154 and the outer advance / retractor 153 and the eccentric disk 152. 157. The top of the connecting rod centering seat 157 is rotatably connected to the second connecting rod 156 and connected to the middle of the second connecting rod 156. The eccentric disk 152 is hinged to one end of the second connecting rod 156 and connected to the first connecting rod 155. The first connecting rod 155 is eccentrically connected to the eccentric disk 152. The two ends of the second connecting rod 156 are respectively hinged to the inner advance seat 154 and the outer advance seat 153 through the third connecting rod 158. The eccentric disk 152 is driven to rotate by the tapping motor 159. The eccentric disk 152 drives the second connecting rod 156 to swing back and forth through the first connecting rod 155, so that the second connecting rod 156 drives the inner advance seat 154 and the outer advance seat 153 to move back and forth through the third connecting rod 158, so that the tapping plate 151 alternately taps the homogenizing bag 7.

[0037] In summary, during implementation, the homogenizing bag 7 containing the incoming material is placed between the partition plates 112 of the turntable 111 by an industrial robot. The first transposition motor 113 drives the turntable 111 to rotate, rotating the homogenizing bag 7 to the placement area. The three-dimensional translation stage 8 drives the first motor gripper 12 to clamp the homogenizing bag 7 on the turntable 111 and place it into the placement area. The liquid addition pipe 17 on the back plate 63 pumps diluent to the homogenizing bag 7 through an external pump. Subsequently, the translation actuator 145 drives the sliding seat 141 to move towards the back plate 63. The lifting frame 142 contacts one side of the homogenizing bag 7 and pulls the homogenizing bag 7 towards the back plate 63. When the homogenizing bag 7 is flattened on the lifting frame 18, the lifting frame 142 and the lifting frame 18... Each of the suction cups 19 of the 8th frame, carrying a homogenizing bag 7, presses and adheres to the space between the suction cups 19 of the other. The suction cups 19 of the lifting frame 142 and the accompanying lifting frame 18 are activated to adsorb the homogenizing bag 7. At the same time, the clamping force of the holding elastic element 146 also drives the homogenizing bag 7 to press against the space between the suction cups 19 on the opposite side. Simultaneously, the tail steering arm 165 swings downward under the restriction of the upper guide frame 1691, causing the slide bar 162 to swing upward. After the slide bar 162 drives the contact roller 161 to swing upward, the contact roller 161 rolls over the homogenizing bag 7, compressing the space in the upper part of the homogenizing bag 7, thereby reducing some of the air inside the homogenizing bag 7. Then, the sliding seat 141 moves forward with the guide seat 143, allowing the wheel 147 to move in the guide channel. 147 moves within the base cavity 1431 towards the wall forming plate 1437, rises through the inclined lifting cavity 1432 to the top of the transfer seat 1435, squeezes the relief plate 1436, and presses it to transition to the lifting cavity 1433 where it remains stationary. Simultaneously, the torque component drives the relief plate 1436 to swing upwards to reset, opening the drop outlet 1438. At this time, the rising of the wheel 147 drives the lifting frame 142 to rise, and the spring compression provides greater clamping force, thereby driving the homogenizing bag 7 and the rear-mounted lifting frame 18, which is pressed down, to rise together. At the same time, the tail directional arm 165 pulls the pulling component 167, and the rise of the homogenizing bag 7 causes the contact roller 161 to rotate. The contact roller 161 squeezes the lower part of the homogenizing bag 7, and the discharged air passes between the two contact rollers 161. The material is discharged through the gap; the eccentric disk 152 is driven to rotate by the tapping motor 159. The eccentric disk 152 drives the second link 156 to swing back and forth through the first link 155, so that the second link 156 drives the inner advance seat 154 and the outer advance seat 153 to move back and forth through the third link 158, so that the tapping plate 151 alternately taps the homogenizing bag 7. If the four tapping plates 151 are marked with the numbers 1, 2, 3, and 4, then each tapping is a combination of tapping 1 and 3, 2 and 4, which mixes the material in the homogenizing bag 7. This automatically makes the homogenizing bag 7 hang with less internal air, and with the tilted tapping plate 151 tapping the hanging homogenizing bag 7 alternately, a better mixing effect is obtained. After mixing, the translation actuator 145 drives the sliding seat 141 to retract. As the roller 147 moves back, it can fall directly through the drop opening 1438. The bottom of the dropping cavity 1434 is level with the bottom of the base cavity 1431. A buffer pad 67 is laid at the bottom of the dropping cavity 1434, allowing the homogenizing bag 7 to fall quickly as the holding elastic element 146 begins to unfold and the applied elastic force begins to shrink. This avoids a decrease in clamping force after the force direction changes, which could lead to the homogenizing bag 7 falling in an alternating manner. The homogenizing bag 7 falls directly within a short stroke; otherwise, an alternating fall could easily twist the homogenizing bag 7, affecting subsequent pipetting and clamping operations. The sliding seat 141 continues to retract, and the roller 147... 47 moves from the placement cavity 1434 to the base cavity 1431, during which the forming plate 1437 is lifted up. The forming plate 1437 swings upward on the inner wall of the guide channel side, and its swing axis is perpendicular to the swing axis of the relief plate 1436. By setting the hinge connection of the forming plate 1437 on the inner wall of the guide channel side, the stress on the transfer seat 1435 is reduced, and the structural strength of the transfer seat 1435 is not insufficient due to both hinge connection points being set on the transfer seat 1435. After the wheel 147 moves into the base cavity 1431, the forming plate 1437 swings down. Optionally, those skilled in the art can let the forming plate 1437 fall naturally, or a torsion spring can be nested at the hinge connection. Then, the suction cups 19 on both sides open the opening of the homogenization bag 7, and the mixture is transferred into the test tube by the pipette 13. The test tube is transferred and then transferred to the inspection and processing section 2 after passing through the culture dish 9. The culture dish 9 is then transferred to the visual counting section 3, and the number of bacteria in the culture dish 9 is calculated by using the existing machine vision solution and the detection counting algorithm with the camera 44.

[0038] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

Claims

1. A visual inspection device for microbial sample processing and culture, comprising a homogenization section, an inspection and processing section, and a visual counting section, wherein the sections are transported between each other via a transfer device, characterized in that: The visual counting unit includes a dish carrier for holding the petri dishes and a camera for counting the petri dishes; The homogenizing unit is mounted on a base, on which a high plate and a back plate are fixed. The homogenizing unit includes a support assembly for carrying the homogenizing bag, a tapping assembly fixed below the high plate, a sealing and lifting assembly fixed above the high plate, a shaping assembly movably mounted on the sealing and lifting assembly, and a pipette for moving and picking up / dropping liquid. The working directions of the sealing and lifting assembly, the shaping assembly, and the tapping assembly all point towards the back plate. There is a gap between the back plate and the high plate, and a placement area is provided on the base within the gap. The sealing and lifting assembly includes a sliding seat, several guide seats fixed on the sliding seat, and a pressing and lifting frame engaged on the guide seats. The front surface of the pressing and lifting frame is provided with several suction cups. The homogenized bag in the placement area is driven by the pressing and lifting frame to perform closing and lifting movements. The pressing and lifting frame drives the homogenized bag to rise on the guide seat for a greater stroke than the downward stroke. A lifting back frame is slidably provided on the back plate to clamp and cooperate with the pressing and lifting frame. The striking assembly is located below the sealing assembly. The striking assembly includes several striking plates and a drive group that drives the striking plates to move alternately at intervals. The striking plates are inclined and the distance between the bottom end and the back plate is smaller than the distance between the top end. The shaping assembly includes a contact roller that oscillates, and the homogenizing bag in the placement area is driven by the contact roller to adjust the internal cavity volume.

2. The visual inspection device for microbial sample processing and culture according to claim 1, characterized in that: The sealing and lifting assembly includes a back seat and several guide seats. The back seat and guide seats are fixed on the sliding seat and located on the front and rear sides of the sliding seat. Several rear lifting frames are fixed on the back of the lifting frame, and a lifting elastic element is fixed between the back seat and the rear lifting frames. The rear display stand is equipped with rotatable wheels on its side. The guide seat has a guide channel for the wheels to engage. The rear display stand is movably engaged with several guide seats through several wheels. The rear display stand is horizontal on several guide seats, and the front plane of the pressure lifting frame is vertical. The pressure lifting frame moves horizontally and vertically within the guide channel through the wheels. The angle between the path of the wheel's descent within the guide channel and the horizontal plane is greater than the angle between the path of its ascent and the horizontal plane.

3. The visual inspection device for microbial sample processing and culture according to claim 2, characterized in that: The guide channel includes a base cavity at a low position within the guide seat, an elevation cavity at a high position, and an inclined elevation cavity that connects the base cavity and the elevation cavity at an angle. A wall-forming plate is hinged to the inner wall of the side of the guide channel, and the wall-forming plate forms the bottom structural wall of the inclined elevation cavity. A transfer seat is fixed on the inner wall of the side of the guide channel. A drop opening is provided between the transfer seat and the lifting cavity. A drop cavity for connecting with the base cavity is provided below the drop opening. A relief plate is hinged to the top of the transfer seat. The relief plate moves within the drop opening and the lifting cavity to connect the inclined lifting cavity and the lifting cavity as well as the open drop opening.

4. The visual inspection device for microbial sample processing and culture according to claim 3, characterized in that: A torsion member is nested between the relief plate and the transfer seat to drive the relief plate to swing upward and open the drop opening. The horizontal height of the bottom of the drop cavity is the same as the horizontal height of the bottom of the base cavity. A buffer pad is laid at the bottom of the drop cavity.

5. The visual inspection device for microbial sample processing and culture according to claim 2, characterized in that: There are two fixed positions for the wheels on the rear display frame. The adjacent wheels have different horizontal heights and different distances from the lifting frame.

6. The visual inspection device for microbial sample processing and culture according to claim 1, characterized in that: The lifting frame includes a follower frame. The front end of the follower frame is provided with several suction cups, which are staggered with the suction cups of the lifting frame. The spacing between the suction cups is greater than the diameter of the suction cups. The back support plate has a first clearance groove, and the bottom of the first clearance groove has a limiting step. The follow-up back frame also includes a back fixing frame located behind the back support plate and fixedly connected to the follow-up frame. The fixing frame is slidably connected to the rear end of the back support plate. The follow-up back frame rests against the limiting step by its own weight and retains the freedom to slide upward.

7. The visual inspection device for microbial sample processing and culture according to claim 1, characterized in that: The sliding seat has an installation groove, the adjusting component is installed in the installation groove on the sliding seat and moves with the sliding seat, and the high plate has a second clearance groove, on which a guide frame is fixed to guide the adjusting component to swing. The shaping assembly includes a contact roller, a slide bar, a rotating bushing, and a pulling member. A truss is horizontally fixed at the front end of the slide bar. Both ends of the contact roller are rotatably connected to the truss and perpendicular to the slide bar. A timing frame is fixed on the sliding seat. The rotating bushing is rotatably mounted on the timing frame. The slide bar is movably nested on the rotating bushing. A tail directional arm is oscillatingly connected to the end of the slide bar. A pull rod is fixed on the tail directional arm. One end of the pulling member is fixed to the pull rod, and the other end is fixed to the timing frame. A guided member is fixed on the other side of the pull rod. The guided member contacts the guide frame, thereby driving the tail directional arm to oscillate.

8. The visual inspection device for microbial sample processing and culture according to claim 7, characterized in that: The fixed connection points of the pull component, the pull rod, and the timing frame are located on both sides of the rotating shaft sleeve, respectively. The connection point between the puller and the pull rod, the connection point between the puller and the timing frame, and the central axis of the rotating shaft sleeve have three projection points on a vertical plane perpendicular to the central axis. The collinear position of the three projection points is the middle position between the puller and the pull rod. The puller provides a pulling force to the pull rod located below the collinear position, causing the slide rod to swing upward. The puller provides a pulling force to the pull rod located above the collinear position, causing the slide rod to swing downward.

9. A visual inspection device for microbial sample processing and culture according to claim 8, characterized in that: The guide frame includes an upper lower guide frame with a downward guide slope and an upper lower guide frame with an upward guide slope. The guided component contacts the upper lower guide frame and swings downward as the sliding seat moves forward, and contacts the upper lower guide frame and swings upward as the sliding seat moves backward. The starting position of the upper guide frame driving the slide bar to swing upward is earlier than the starting position of the lifting frame rising on the guide seat.

10. A visual inspection device for microbial sample processing and culture according to claim 1, characterized in that: The driving group of the striking assembly includes a striking motor fixed on the base and an eccentric disk fixed on the rotating shaft of the striking motor. There are two eccentric disks, which are located on both sides of the striking motor. There are four striking plates, which are divided into two groups. Each group of striking plates is fixedly connected to the inner advance seat and the outer advance seat on one side of the striking motor. The inner advance seat and the outer advance seat are slidably mounted on the base. A connecting rod centering seat is fixed on the base between the inner and outer advance seats and the eccentric disk. The top of the connecting rod centering seat is rotatably connected to a second connecting rod and connected to the middle of the second connecting rod. The eccentric disk is connected to one end of the second connecting rod and a first connecting rod. The two ends of the second connecting rod are respectively connected to the inner and outer advance seats through a third connecting rod. The bottom of the tapping plate has a raised section for contacting the bottom of the homogenizing bag.

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