A cell culture device

Abstract

The application discloses a cell culture device, which comprises a body, a culture tank is arranged on the top of the body, an auxiliary assembly is arranged on the top of the culture tank, a water bath assembly is arranged on the bottom of the culture tank, a transmission assembly is arranged on the axis of the culture tank, an oscillation mechanism is arranged in the culture tank and is driven by the transmission assembly, an oscillation table is in transmission connection with the port of the oscillation mechanism, a rotary table is in rotation connection with the inner wall of the oscillation table and is driven by the transmission assembly, a plurality of culture mechanisms are arranged on the circumferential side of the rotary table in a circumferential array, an inner gear ring in transmission connection with the culture mechanisms is fixedly arranged on the inner wall of the body, and an air clamp assembly in communication with the culture mechanisms is arranged on the bottom of the body. The cell culture test tube is subjected to multiple-effect movement during culture, so that the concentration uniformity and temperature uniformity of the liquid in the cell culture test tube are improved, and the culture efficiency and culture effect of cells are improved.

Description

Technical Field

[0001] This invention relates to the field of cell culture technology, and more specifically, to a cell culture apparatus. Background Technology

[0002] Cell culture technology is an important and commonly used technique in cell biology research. Through cell culture, a large number of cells can be obtained, and cell signal transduction, cellular synthesis and metabolism, cell growth and proliferation can be studied. Cell culture devices are required during the cell culture process. Common cell culture devices include cell incubators. Cell incubators are advanced instruments for culturing cells, tissues and bacteria, and are essential key equipment for conducting immunology, oncology, genetics and bioengineering.

[0003] In the prior art, patent document CN217948152U discloses a cell culture device, including a mounting frame, an incubator, a liquid injection mechanism, a culture mechanism, and a rotation mechanism. The liquid injection mechanism includes a water pump, a liquid injection tank, an electric push rod, a connecting tube, a liquid injection tray, a solenoid valve, and a nozzle. The above-mentioned culture device can add culture medium to individual cells according to the cell condition, which is convenient for adding according to different cell conditions, thereby further improving the success rate of cell culture. However, the above-mentioned culture device is not convenient for improving the temperature uniformity and concentration uniformity of cell culture through multi-directional movement of the culture tube. Based on this, the present invention provides a cell culture device to solve the technical problems mentioned in the background art. Summary of the Invention

[0004] (a) Technical problems to be solved In view of the shortcomings of the prior art, the purpose of this invention is to provide a cell culture device. This invention improves the uniformity of liquid concentration and temperature inside the cell culture tube by the occurrence of multi-effect motion during cell culture test tube culture, thereby improving the cell culture efficiency and culture effect.

[0005] (II) Technical Solution

[0006] To achieve the above objectives, the present invention provides the following technical solution: a cell culture device, comprising a body, a culture tank mounted on the top of the body, an auxiliary component mounted on the top of the culture tank, a water bath component mounted on the bottom of the culture tank, a transmission component mounted on the axial position of the culture tank, an oscillation mechanism driven by the transmission component mounted inside the culture tank, an oscillation table driven by the transmission component being connected to the port of the oscillation mechanism, a rotation frame driven by the transmission component being rotatably connected to the inner wall of the oscillation table, a set of culture mechanisms arranged in a circular array mounted on the circumferential side of the rotation frame, an internal gear ring driven by the culture mechanism being fixedly mounted on the inner wall of the body, and an air clamp component communicating with the culture mechanism being mounted on the bottom of the body.

[0007] As a preferred embodiment, the auxiliary components include a door installed on the top surface of the culture tank, a liquid replenishment pipe fixedly installed on the top of the culture tank, and a vent valve. The top surface of the culture tank is fixedly provided with two symmetrically arranged observation areas, which are made of transparent glass. The periphery of the culture tank is provided with two symmetrically arranged operating tubes, and the tail ends of the two operating tubes are connected to operating gloves.

[0008] As a preferred embodiment, the water bath assembly includes a water bath chamber located inside the culture tank, an electric heating ring tube fixed to the bottom of the water bath chamber, and a temperature probe fixedly installed at the bottom of the machine body for monitoring the temperature inside the water bath chamber. A drain valve connected to the water bath chamber is fixedly installed at the bottom of the machine body, and a microcontroller electrically connected to the temperature probe is fixedly installed on the periphery of the culture tank.

[0009] As a preferred embodiment, the transmission assembly includes a transmission sleeve a rotatably connected to the inside of the machine body, a transmission sleeve b rotatably connected to the top of the culture tank, and a servo motor fixed inside the machine body. The output shaft end of the servo motor is fixedly equipped with an active bevel gear, and the peripheral side of the transmission sleeve a is fixedly equipped with a driven bevel gear that meshes with the active bevel gear. The opposing surfaces of the transmission sleeve a and the transmission sleeve b are slidably connected and driven by a rotary vibration shaft. The peripheral side of the rotary vibration shaft is rotatably connected to the oscillation table, and the peripheral side of the rotary vibration shaft is fixedly connected to the indexing frame.

[0010] As a preferred embodiment, several sets of circumferentially arrayed baffles are installed on the circumferential side of the transmission sleeve a and at positions corresponding to the inside of the water bath cavity.

[0011] As a preferred embodiment, the transmission sleeve a has a transmission guide groove a with openings at both ends fixedly formed inside, and the transmission sleeve a has a transmission guide groove b with an opening at the bottom fixedly formed inside. The inner walls of the transmission guide groove a and the transmission guide groove b are slidably connected to the rotary vibration shaft. The cross-sections of the rotary vibration shaft, the transmission guide groove a, and the transmission guide groove b are all regular polygons.

[0012] As a preferred embodiment, the oscillation mechanism includes a linkage bevel gear fixedly installed on the circumferential side of the transmission sleeve b, a guide rod fixedly installed on the top of the culture tank, and a gear shaft rotatably connected to the inside of the culture tank. The inner wall of the oscillation table is slidably connected to the guide rod. A return spring is sleeved on the circumferential side of the guide rod at a position corresponding to the bottom of the oscillation table. An oscillation gear plate is fixedly installed on the top surface of the oscillation table. A passive bevel gear that meshes with the linkage bevel gear and a half-gear that is driven by the oscillation gear plate are fixedly installed on the circumferential side of the gear shaft. The axes of the guide rod and the oscillation gear plate are parallel to the axis of the rotary vibration shaft, and the axis of the gear shaft is perpendicular to the axis of the rotary vibration shaft.

[0013] As a preferred embodiment, the culture mechanism includes a pendulum ring, a rotating ring rotatably connected to the inner wall of a rotation frame, a fixed bevel gear ring fixed to the surface of the rotation frame and coaxially arranged with the rotating ring, and an air supply ring tube. A spin gear connected to the internal gear ring is fixedly installed on the circumferential side of the rotating ring. Two pendulum shafts are fixedly installed on the circumferential side of the pendulum ring, and the circumferential sides of both pendulum shafts are rotatably connected to the rotating ring. A half-bevel gear connected to the fixed bevel gear ring is fixedly installed on the circumferential side of one pendulum shaft. A torsion spring is fixedly installed between the other pendulum shaft and the opposite surface of the rotating ring. A receiving ring groove is fixedly opened inside the pendulum ring. A ring clamp is fixedly installed on the inner wall of the receiving ring groove. A gas distribution ring is rotatably connected to the inner wall of the air supply ring tube. An air passage is fixedly opened inside one of the pendulum shafts. The back of the ring clamp is rotatably connected to the gas distribution ring through the air passage. The surface of the air supply ring tube is connected to the air clamp assembly. The inner wall of the ring clamp holds the cell culture test tube.

[0014] As a preferred embodiment, the axis of the rotating ring is parallel to the axis of the rotating shaft, the axis of the pendulum shaft is perpendicular to the axis of the rotating shaft, the inner wall of the ring clamp is evenly distributed with friction textures, and the bottom surface of the pendulum ring is fixedly installed with a bracket that cooperates with the cell culture test tube.

[0015] As a preferred embodiment, the air clamp assembly includes an air pump fixed inside the machine body, an air distribution chamber opened inside the indexing frame, and an air delivery channel opened on the inner wall of the rotating shaft. The air outlet port of the air pump is connected to the air distribution chamber through the air delivery channel, and the surface of the air distribution chamber is fixedly connected to the air delivery ring pipe through a flexible hose.

[0016] (III) Beneficial Effects

[0017] Compared with the prior art, the present invention provides a cell culture device with the following beneficial effects: In operation, the cells to be cultured and the cell culture medium are injected into the cell culture tubes in a quantitative ratio. The cell culture tubes containing the cells and cell culture medium are then placed into the respective swing rings. After the cell culture tubes are placed, the air pump inflates, and then the cell culture tubes are effectively clamped by the ring clamps. After the air pump has been running for a specified time, the cell culture operation is completed. During cell culture, the servo motor operates periodically. Within the working cycle of the servo motor, the rotating frame undergoes a revolution motion on one hand, and on the other hand, it drives the rotating frame to oscillate back and forth within a set stroke. When the rotating frame revolves, the rings rotate on their own axis, and the swing rings oscillate back and forth within a set angle range. Through the occurrence of multiple effects of motion during cell culture, the uniformity of the concentration and temperature of the liquid inside the cell culture tubes is improved, thereby improving the cell culture efficiency and effect. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the structure of a cell culture device according to the present invention; Figure 2 For the present invention Figure 1 A schematic diagram of the cross-sectional structure; Figure 3 For the present invention Figure 2 A magnified schematic diagram of the local structure at point A; Figure 4 For the present invention Figure 2 A magnified view of the structure at point B in the middle; Figure 5 For the present invention Figure 2 A magnified schematic diagram of the local structure at point C; Figure 6 For the present invention Figure 2 A magnified schematic diagram of the local structure at point D; Figure 7 This is a schematic diagram of the structure of the oscillation table and the indexing frame of the present invention; Figure 8 This is a schematic diagram of the structure of the rotary vibrating shaft and the turbulence rod of the present invention; Figure 9 This is a schematic diagram of the structure of the torsion spring and the ring clamping bladder of the present invention; Figure 10 This is a schematic diagram of the structure of the pendulum ring and pendulum shaft of the present invention.

[0019] In the diagram: 1. Body; 2. Culture tank; 3. Shaking table; 4. Rotating rack; 5. Internal gear ring; 6. Door; 7. Liquid replenishment tube; 8. Vent valve; 9. Operating tube; 10. Heating ring tube; 11. Microcontroller; 12. Transmission sleeve a; 13. Transmission sleeve b; 14. Servo motor; 15. Rotating shaft; 16. Baffle rod; 17. Guide rod; 18. Return spring; 19. Shaking tooth plate; 20. Half-lobe gear; 21. Swing ring; 22. Rotating ring; 23. Fixed bevel gear ring; 24. Gas supply ring tube; 25. Spinning gear; 26. Half-bevel gear; 27. Torsion spring; 28. Ring clamp; 29. ​​Gas distribution ring; 30. Cell culture tube; 31. Swing shaft; 32. Bracket; 33. Air pump. Detailed Implementation

[0020] The present invention will be further described and illustrated below with reference to specific embodiments and the accompanying drawings: Please see Figure 1-10 The present invention is a cell culture device, the technical solution of which includes a body 1, a culture tank 2 installed on the top of the body 1, and auxiliary components installed on the top of the culture tank 2. The auxiliary components include a door 6 installed on the top surface of the culture tank 2, a liquid replenishment pipe 7 fixedly installed on the top of the culture tank 2, and a vent valve 8. Two symmetrically arranged observation areas are fixedly provided on the top surface of the culture tank 2. The observation areas are made of transparent glass. Two symmetrically arranged operation pipes 9 are provided on the periphery of the culture tank 2. The tail ends of the two operation pipes 9 are connected to operation gloves.

[0021] The addition of the replenishment tube 7 enables rapid replenishment and filling of the working solution into the cell culture tube 30. The oxygen content inside the culture tank 2 can be adjusted by setting the vent valve 8. By using operating gloves, cell sampling can be performed on cell culture tube 30 without opening the chamber door; A water bath assembly is installed at the bottom of culture tank 2; The water bath assembly includes a water bath chamber located inside the culture tank 2, an electric heating ring tube 10 fixed to the bottom of the water bath chamber, and a temperature probe fixedly installed at the bottom of the body 1 for monitoring the temperature inside the water bath chamber. A drain valve connected to the water bath chamber is fixedly installed at the bottom of the body 1, and a microcontroller 11 electrically connected to the temperature probe is fixedly installed on the periphery of the culture tank 2.

[0022] During operation, the water bath chamber is filled with a sufficient amount of water bath liquid. The temperature probe is used to monitor the liquid temperature inside the water bath chamber in real time and feed back the monitored temperature data to the microcontroller 11 in real time. The microcontroller 11 controls the heating state of the electric heating ring tube 10 based on the data feedback from the temperature probe, thereby maintaining the water bath liquid at a constant temperature, thus realizing the constant temperature culture of cells. Both the temperature probe and the microcontroller 11 can be customized or selected according to actual needs; A transmission assembly is installed at the axial position of the culture tank 2. An oscillation mechanism driven by the transmission assembly is installed inside the culture tank 2. The port of the oscillation mechanism is connected to the oscillation table 3. The inner wall of the oscillation table 3 is rotatably connected to a rotary table 4 driven by a transmission assembly; The transmission components include a transmission sleeve a12 rotatably connected to the inside of the machine body 1, a transmission sleeve b13 rotatably connected to the top of the culture tank 2, and a servo motor 14 fixed inside the machine body 1. The output shaft end of the servo motor 14 is fixedly mounted with an active bevel gear. The peripheral side of the transmission sleeve a12 is fixedly mounted with a driven bevel gear that meshes with the active bevel gear. The relative surfaces of the transmission sleeve a12 and the transmission sleeve b13 are slidably connected and are coupled with a rotary vibration shaft 15. The peripheral side of the rotary vibration shaft 15 is rotatably connected to the oscillation table 3, and the peripheral side of the rotary vibration shaft 15 is fixedly connected to the indexing frame 4. The transmission sleeve a12 has a transmission guide groove a with openings at both ends, and the transmission sleeve a12 has a transmission guide groove b with openings at the bottom. The inner walls of the transmission guide groove a and the transmission guide groove b are slidably connected to the rotary vibration shaft 15. The cross-sections of the rotary vibration shaft 15, the transmission guide groove a and the transmission guide groove b are all regular polygons. Four sets of circumferentially arrayed baffles 16 are installed on the circumferential side of the transmission sleeve a12 and at positions corresponding to the inside of the water bath chamber. The oscillation mechanism includes a linkage bevel gear fixedly installed on the circumferential side of the transmission sleeve b13, a guide rod 17 fixedly installed on the top of the culture tank 2, and a gear shaft rotatably connected to the inside of the culture tank 2. The inner wall of the oscillation table 3 is slidably connected to the guide rod 17. A return spring 18 is sleeved on the circumferential side of the guide rod 17 and at the position corresponding to the bottom of the oscillation table 3. An oscillation gear plate 19 is fixedly installed on the top surface of the oscillation table 3. A passive bevel gear that meshes with the linkage bevel gear and a half-gear 20 that is transmitted to the oscillation gear plate 19 are fixedly installed on the circumferential side of the gear shaft. The axes of the guide rod 17 and the oscillation gear plate 19 are parallel to the axis of the rotary vibration shaft 15, and the axis of the gear shaft is perpendicular to the axis of the rotary vibration shaft 15.

[0023] A set of culture mechanisms arranged in a circular array are installed on the circumferential side of the rotation rack 4. An internal gear ring 5 that is connected to the culture mechanism is fixedly installed on the inner wall of the machine body 1. An air clamp assembly that is connected to the culture mechanism is installed at the bottom of the machine body 1.

[0024] The cultivation mechanism includes a swing ring 21, a rotating ring 22 rotatably connected to the inner wall of the indexing frame 4, a fixed bevel gear ring 23 fixed to the surface of the indexing frame 4 and coaxially arranged with the rotating ring 22, and an air supply ring pipe 24. The axis of the rotating ring 22 is parallel to the axis of the rotating shaft 15. A spin gear 25 that is connected to the internal gear ring 5 is fixedly installed on the circumferential side of the rotating ring 22. Two swing shafts 31 are fixedly installed on the circumferential side of the swing ring 21. The axis of the swing shaft 31 is perpendicular to the axis of the rotating shaft 15. Both circumferential surfaces of the two swing shafts 31 are rotatably connected to the ring 22. A half-bevel gear 26 that is connected to the fixed bevel gear ring 23 is fixedly installed on the circumferential surface of one swing shaft 31. A torsion spring 27 is fixedly installed between the opposing surfaces of the other swing shaft 31 and the ring 22. By using the half-bevel gear 26 and the torsion spring 27, the swing ring 21 can reciprocate within a set angle range when the rotating ring 22 rotates. The pendulum ring 21 has a fixed storage ring groove inside, and a ring clamp 28 is fixedly installed on the inner wall of the storage ring groove. The inner wall of the gas supply ring pipe 24 is rotatably connected to a gas distribution ring 29. The pendulum shaft 31 has a fixed air passage inside. The back of the ring clamp 28 is rotatably connected to the gas distribution ring 29 through the air passage. The surface of the gas supply ring pipe 24 is connected to the gas clamp assembly. The inner wall of the ring clamp 28 holds the cell culture test tube 30.

[0025] The inner wall of the ring clamp 28 is evenly distributed with friction texture, and the bottom surface of the swing ring 21 is fixedly installed with a bracket 32 ​​that cooperates with the cell culture test tube 30.

[0026] The air clamp assembly includes an air pump 33 fixed inside the machine body 1, an air distribution chamber opened inside the indexing frame 4, and an air delivery channel opened on the inner wall of the rotating shaft 15. The air outlet of the air pump 33 is connected to the air distribution chamber through the air delivery channel, and the surface of the air distribution chamber is fixedly connected to the air delivery ring pipe 24 through a hose.

[0027] The working principle of this invention is as follows: This device is mainly suitable for the culture of aerobic cells. During the culture process, the cells to be cultured and the cell culture medium are injected into the cell culture tube 30 in a quantitative ratio. The cell culture tube 30 containing the cells and cell culture medium is then placed into each of the swing rings 21. After the cell culture tube 30 is placed, the air pump 33 inflates the tube and then effectively clamps the cell culture tube 30 through the ring clamp 28. After the air pump 33 has worked for a specified time, the culture process of the cell culture tube 30 is completed. During cell culture, the servo motor 14 works periodically. Within the working cycle of the servo motor 14, the rotating frame 4 revolves on one hand and drives the rotating frame 4 to oscillate back and forth within a set stroke on the other hand. When the rotating frame 4 revolves, the rotating ring 22 rotates on its own axis, and the swing ring 21 oscillates back and forth within a set angle range. Through the occurrence of multiple effects of motion during the culture of the cell culture tube 30, the uniformity of the concentration and temperature of the liquid inside the cell culture tube 30 is improved.

[0028] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A cell culture device, comprising an organism (1), characterized in that: A culture tank (2) is installed on the top of the body (1). An auxiliary component is installed on the top of the culture tank (2). A water bath component is installed on the bottom of the culture tank (2). A transmission component is installed on the axis of the culture tank (2). An oscillation mechanism driven by the transmission component is installed inside the culture tank (2). An oscillation table (3) is connected to the port of the oscillation mechanism. A rotary table (4) driven by the transmission component is rotatably connected to the inner wall of the oscillation table (3). A set of culture mechanisms arranged in a circular array is installed on the circumferential side of the rotary table (4). An internal gear ring (5) connected to the culture mechanism is fixedly installed on the inner wall of the body (1). An air clamp component connected to the culture mechanism is installed at the bottom of the body (1).

2. The cell culture apparatus according to claim 1, characterized in that: The auxiliary components include a door (6) installed on the top surface of the culture tank (2), a liquid replenishment tube (7) fixedly installed on the top of the culture tank (2), and a vent valve (8). The top surface of the culture tank (2) is fixedly provided with two symmetrically arranged observation areas, which are made of transparent glass. The circumferential side of the culture tank (2) is provided with two symmetrically arranged operation tubes (9), and the tail ends of the two operation tubes (9) are connected to operation gloves.

3. The cell culture apparatus according to claim 1, characterized in that: The water bath assembly includes a water bath chamber opened inside the culture tank (2), an electric heating ring tube (10) fixed at the bottom of the water bath chamber, and a temperature probe fixedly installed at the bottom of the body (1) for monitoring the temperature inside the water bath chamber. A drain valve connected to the water bath chamber is fixedly installed at the bottom of the body (1), and a microcontroller (11) electrically connected to the temperature probe is fixedly installed on the periphery of the culture tank (2).

4. The cell culture apparatus according to claim 3, characterized in that: The transmission components include a transmission sleeve a (12) rotatably connected to the inside of the body (1), a transmission sleeve b (13) rotatably connected to the top of the culture tank (2), and a servo motor (14) fixed inside the body (1). The output shaft end of the servo motor (14) is fixedly equipped with an active bevel gear. The peripheral side of the transmission sleeve a (12) is fixedly equipped with a driven bevel gear that meshes with the active bevel gear. The relative surfaces of the transmission sleeve a (12) and the transmission sleeve b (13) are slidably connected and are coupled with a rotary vibrating shaft (15). The peripheral side of the rotary vibrating shaft (15) is rotatably connected to the oscillation table (3), and the peripheral side of the rotary vibrating shaft (15) is fixedly connected to the indexing frame (4).

5. A cell culture apparatus according to claim 4, characterized in that: Several sets of circumferentially arranged baffle rods (16) are installed on the circumferential side of the transmission sleeve a (12) and at the position corresponding to the inside of the water bath cavity.

6. The cell culture apparatus according to claim 4, characterized in that: The transmission sleeve a (12) has a transmission guide groove a with openings at both ends, and the transmission sleeve a (12) has a transmission guide groove b with an opening at the bottom. The inner walls of the transmission guide groove a and the transmission guide groove b are slidably connected to the rotary vibration shaft (15). The cross-sections of the rotary vibration shaft (15), the transmission guide groove a, and the transmission guide groove b are all regular polygons.

7. A cell culture apparatus according to claim 6, characterized in that: The oscillation mechanism includes a linkage bevel gear fixedly installed on the circumferential side of the transmission sleeve b (13), a guide rod (17) fixedly installed on the top of the culture tank (2), and a gear shaft rotatably connected to the inside of the culture tank (2). The inner wall of the oscillation table (3) is slidably connected to the guide rod (17). A return spring (18) is sleeved on the circumferential side of the guide rod (17) and at the position corresponding to the bottom of the oscillation table (3). An oscillation gear plate (19) is fixedly installed on the top surface of the oscillation table (3). A passive bevel gear that meshes with the linkage bevel gear and a half-gear (20) that is connected to the oscillation gear plate (19) are fixedly installed on the circumferential side of the gear shaft. The axes of the guide rod (17) and the oscillation gear plate (19) are parallel to the axis of the rotary vibration shaft (15). The axis of the gear shaft is perpendicular to the axis of the rotary vibration shaft (15).

8. The cell culture apparatus according to claim 1, characterized in that: The cultivation mechanism includes a swing ring (21), a rotating ring (22) rotatably connected to the inner wall of the indexing frame (4), a fixed bevel gear ring (23) fixed to the surface of the indexing frame (4) and coaxially arranged with the rotating ring (22), and an air supply ring pipe (24). A spin gear (25) that is driven by the internal gear ring (5) is fixedly installed on the circumferential side of the rotating ring (22). Two swing shafts (31) are fixedly installed on the circumferential side of the swing ring (21). The circumferential sides of the two swing shafts (31) are rotatably connected to the rotating ring (22). A half bevel gear that is driven by the fixed bevel gear ring (23) is fixedly installed on the circumferential side of one of the swing shafts (31). (26) A torsion spring (27) is fixedly installed between the opposing surfaces of the other swing shaft (31) and the rotating ring (22). A receiving ring groove is fixedly opened inside the swing ring (21). A ring clamp (28) is fixedly installed on the inner wall of the receiving ring groove. A gas distribution ring (29) is rotatably connected to the inner wall of the gas supply ring pipe (24). A gas passage is fixedly opened inside the swing shaft (31). The back of the ring clamp (28) is rotatably connected to the gas distribution ring (29) through the gas passage. The surface of the gas supply ring pipe (24) is connected to the gas clamp assembly. The inner wall of the ring clamp (28) holds the cell culture test tube (30).

9. A cell culture apparatus according to claim 8, characterized in that: The axis of the rotating ring (22) is parallel to the axis of the rotating shaft (15), the axis of the pendulum shaft (31) is perpendicular to the axis of the rotating shaft (15), the inner wall of the ring clamp (28) is evenly distributed with friction textures, and the bottom surface of the pendulum ring (21) is fixedly installed with a bracket (32) that cooperates with the cell culture test tube (30).

10. A cell culture apparatus according to claim 9, characterized in that: The air clamp assembly includes an air pump (33) fixed inside the machine body (1), an air distribution chamber opened inside the rotary frame (4), and an air delivery channel opened on the inner wall of the rotary shaft (15). The air outlet of the air pump (33) is connected to the air distribution chamber through the air delivery channel, and the surface of the air distribution chamber is fixedly connected to the air delivery ring pipe (24) through a hose.

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