Application of rice LRR type receptor-like kinase OsLRR-RLK-R11 in regulating rice blast resistance

Abstract

The application discloses application of a rice LRR type receptor-like kinase OsLRR-RLK-R11 in regulating rice blast resistance, and discloses and proves for the first time that the OsLRR-RLK-R11 gene is a positive regulation factor of rice blast resistance, provides a new gene resource for plant immunity research, and fills the blank of the regulation function of the gene in rice blast resistance; the application proves the positive regulation function of the OsLRR-RLK-R11 gene in rice blast resistance from two aspects of positive and negative through CRISPR / Cas9 gene knockout and transgenic technology, and forms a complete gene function evidence chain, the gene can be applied to cultivation of rice varieties resistant to rice blast through transgenic technology, gene editing technology or molecular marker assisted selection, and has important industrial application prospects; the application provides a new example and clue for the function research of LRR-RLK family members in plant innate immunity.

Description

Technical Field

[0001] This invention relates to the fields of agricultural biotechnology and plant genetics and breeding technology, and more particularly to the application of a rice LRR-type receptor kinase OsLRR-RLK-RI1 in regulating rice blast resistance. More specifically, this invention relates to OsLRR-RLK-RI1 ( Oryza sativa The Leucine-Rich Repeat Receptor-Like Kinase Resistance-Inducing1 gene serves as a positive regulator of rice blast resistance, and its application in improving rice blast resistance and breeding disease-resistant varieties. Background Technology

[0002] Rice blast is caused by the rice blast fungus ( ). Magnaporthe oryzae This disease is caused by a devastating fungal infection, seriously threatening rice production and food security. Currently, its control mainly relies on two approaches: chemical fungicides and the breeding of resistant varieties. While chemical control is effective in the short term, long-term use can lead to drug resistance in the pathogen and pose potential risks to the ecological environment and food safety. In contrast, breeding and promoting resistant varieties is widely recognized as the most economical, effective, and environmentally friendly control strategy.

[0003] Cloning and identifying rice blast resistance genes is the foundation and core of molecular breeding for disease resistance. To date, over 100 rice blast resistance genes have been identified, with more than 40 successfully cloned. These cloned resistance genes mainly encode two types of proteins: one type consists of nucleotide-binding sites and leucine-rich repeat (NBS-LRR) receptor proteins, which trigger immune responses by recognizing pathogen effector factors; the other type consists of receptor-like kinases (RLKs) and related proteins, including B-lectin receptor kinases (such as Pi-d2), LRR-type receptor kinases (such as Pi65 and OsBRR1), cell wall-associated kinases (such as Pb4), and receptor-like cytoplasmic kinases (such as BSR1). However, the physiological races of rice blast fungus exhibit frequent variation, and resistance genes often lose their effectiveness after several years of promotion due to the adaptive evolution of the pathogen. Therefore, the continuous discovery and identification of new resistance gene resources, especially those with different mechanisms of action, is of significant theoretical and practical value for achieving sustainable and green control of rice blast.

[0004] Leucine-rich repeat receptor-like kinases (LRR-RLKs) are the largest family of transmembrane receptor kinases in plants. Members of the LRR-RLK family are widely involved in plant growth and development, hormone signal transduction, and responses to biotic and abiotic stresses. In plant immunity, LRR-RLKs act as pattern recognition receptors (PRRs), activating the plant's innate immune response (PTI) by recognizing pathogen-associated molecular patterns (PAMPs), forming the first line of defense against pathogen invasion. Currently, relatively few LRR-RLK genes with rice blast resistance function have been reported. For example, OsBRR1 has been identified as an LRR-RLK involved in the rice blast resistance response; plants with suppressed OsBRR1 expression are susceptible to rice blast fungus, while overexpression enhances resistance. Pi65 is a novel rice blast resistance gene cloned from japonica rice, encoding an LRR-RLK domain protein; its knockout mutants lose resistance to rice blast. Nevertheless, the rice genome encodes hundreds of LRR-RLK family members, and the biological functions of the vast majority of these members in rice blast resistance remain unknown.

[0005] Currently, there are no publicly available documents or patent reports on rice LRR-RLK family member OsLRR-RLK-RI1 ( O ryza s ativa L eucine- R ich R epeat R eceptor- L ike K inase R esistance- I nducing 1 The LRR-RLK-RI1 gene and its encoded proteins are involved in regulating rice resistance to rice blast. Therefore, cloning and identifying the biological function of the OsLRR-RLK-RI1 gene in rice blast resistance is of great significance for enriching disease resistance gene resources, revealing new mechanisms of the LRR-RLK family in plant immunity, and providing new gene targets for molecular breeding of rice disease resistance. Summary of the Invention

[0006] In view of this, the purpose of this invention is to propose an application of rice LRR-type receptor kinase OsLRR-RLK-RI1 in regulating rice blast resistance. This scheme is based on the rice LRR-type receptor kinase gene OsLRR-RLK-RI1 and confirms that it positively regulates rice resistance to rice blast, so as to solve the problems of insufficient existing rice blast resistance gene resources and unknown functions of most LRR-RLK family members in rice blast resistance, thereby providing new gene resources and technical means for molecular breeding of rice disease resistance.

[0007] To achieve the above-mentioned technical objectives, the technical solution adopted by this invention is as follows: Firstly, this scheme proposes a rice LRR-type receptor kinase OsLRR-RLK-RI1, whose amino acid sequence is one of the following: a) Has the amino acid sequence shown in SEQ ID NO: 2; b) A variant having at least 90%, 95%, 98% or 99% sequence identity with the amino acid sequence shown in SEQ ID NO: 2, and retaining the function of regulating rice resistance to rice blast.

[0008] Secondly, this scheme proposes a gene encoding the rice LRR-type receptor kinase OsLRR-RLK-RI1 described above, which has the nucleotide sequence shown in SEQ ID NO: 1.

[0009] Thirdly, this scheme proposes a rice LRR-type receptor kinase OsLRR-RLK-RI1 gene, whose nucleotide sequence is one of the following: a) Has a nucleotide sequence as shown in SEQ ID NO: 1; b) A variant having at least 90%, 95%, 98% or 99% sequence identity with the nucleotide sequence shown in SEQ ID NO: 1 and encoding a protein that retains the function of regulating rice resistance to rice blast.

[0010] Fourthly, this scheme proposes a recombinant expression vector containing the gene described above or the rice LRR-type receptor kinase OsLRR-RLK-RI1 gene.

[0011] Fifthly, this scheme proposes a host cell that contains the recombinant expression vector described above, or whose genome integrates the gene described above or the rice LRR-type receptor kinase OsLRR-RLK-RI1 gene described above.

[0012] Sixthly, this scheme proposes the use of the aforementioned gene or the rice LRR-type receptor kinase OsLRR-RLK-RI1 gene in regulating rice resistance to rice blast; wherein the regulation is positive regulation, which enhances rice resistance to rice blast by increasing the expression level or activity of the gene-encoded rice LRR-type receptor kinase OsLRR-RLK-RI1.

[0013] Seventhly, this scheme proposes the application of the aforementioned gene or the rice LRR-type receptor kinase OsLRR-RLK-RI1 gene in the breeding of rice varieties resistant to rice blast. The application includes: enhancing the resistance of rice to rice blast by overexpressing the gene and / or increasing the expression level or activity of the gene-encoded rice LRR-type receptor kinase OsLRR-RLK-RI1.

[0014] Seventhly, this scheme proposes a method to improve the resistance of rice to rice blast, which includes: overexpressing the rice LRR-type receptor kinase OsLRR-RLK-RI1 gene in rice, or increasing the expression level or activity of rice LRR-type receptor kinase OsLRR-RLK-RI1; the overexpression includes transforming an expression vector containing the rice LRR-type receptor kinase OsLRR-RLK-RI1 gene into rice cells to obtain transgenic rice plants with increased OsLRR-RLK-RI1 gene expression level; The nucleotide sequence of the rice LRR-type receptor kinase OsLRR-RLK-RI1 gene is one of the following: a) Has a nucleotide sequence as shown in SEQ ID NO: 1; b) A variant having at least 90%, 95%, 98% or 99% sequence identity with the nucleotide sequence shown in SEQ ID NO: 1, and encoding a variant that retains the functional protein regulating rice resistance to rice blast. The amino acid sequence of the rice LRR-type receptor kinase OsLRR-RLK-RI1 is one of the following: a) Has the amino acid sequence shown in SEQ ID NO: 2; b) A variant having at least 90%, 95%, 98% or 99% sequence identity with the amino acid sequence shown in SEQ ID NO: 2, and retaining the function of regulating rice resistance to rice blast.

[0015] Eighthly, this plan proposes the application of the aforementioned gene or the rice LRR-type receptor kinase OsLRR-RLK-RI1 gene in the preparation of transgenic plants resistant to rice blast.

[0016] By adopting the above technical solution, the present invention has the following beneficial effects compared with the prior art: 1. First time revealing gene function: This invention reveals and confirms for the first time that the OsLRR-RLK-RI1 gene is a positive regulator of rice blast resistance, providing new gene resources for plant immunity research and filling the gap in the gene's regulatory function of rice blast resistance.

[0017] 2. Dual Experiments, Sufficient Evidence: This invention systematically demonstrates the positive regulatory function of the OsLRR-RLK-RI1 gene in rice blast resistance from both positive and negative perspectives through CRISPR / Cas9 gene knockout and overexpression transgenic technology. Knocking out this gene makes rice more susceptible to rice blast fungus, while overexpression of this gene significantly enhances rice's resistance, forming a complete chain of evidence for gene function.

[0018] 3. Clear application value and broad breeding prospects: The identification of the OsLRR-RLK-RI1 gene provides a new gene target for molecular breeding of rice disease resistance. This gene can be applied to the breeding of rice varieties resistant to rice blast through transgenic technology, gene editing technology, or molecular marker-assisted selection, and has important prospects for industrial application.

[0019] 4. Expanding our understanding of the LRR-RLK family's immune function: This invention provides new examples and clues for the functional study of LRR-RLK family members in plant innate immunity, enriching our understanding of the LRR-RLK family's involvement in plant-pathogen interaction mechanisms. Attached Figure Description

[0020] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0021] Figure 1 This is a diagram analyzing the expression patterns of the OsLRR-RLK-RI1 gene in different rice tissues. Figure 2 It is a CRISPR / Cas9 knockout mutant of the OsLRR-RLK-RI1 gene ( oslrr-rlk-ri1-1 and oslrr- rlk-ri1-2 Photographs of lesion phenotypes after inoculation with rice blast fungus, and statistical charts of the number of lesions and the area of ​​lesions by proportion; Figure 3These are photographs of lesion phenotypes, number of lesions, and statistical charts of lesion area proportions in OsLRR-RLK-RI1 gene overexpressing plants (OsLRR-RLK-RI1-OE-1 and OsLRR-RLK-RI1-OE-2) after inoculation with rice blast fungus. Figure 4 This is a subcellular localization map of the OsLRR-RLK-RI1 protein in rice protoplasts; Figure 5 This is a temporal analysis diagram of the expression level of the OsLRR-RLK-RI1 gene after infection with rice blast fungus. Detailed Implementation

[0022] The present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be particularly noted that the following embodiments are for illustrative purposes only and do not limit the scope of the invention. Similarly, the following embodiments are only some, not all, embodiments of the present invention, and all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0023] In addition, experimental methods not specified in the examples are generally performed under conventional conditions, such as those described in Molecular Cloning: A Laboratory Manual by Sambrook et al., or as recommended by the manufacturer.

[0024] Example 1: Cloning and Sequence Analysis of the OsLRR-RLK-RI1 Gene 1. Rice materials and RNA extraction: Using rice variety 'Nipponbare' ( Oryza sativa L. ssp. japonica 'Nipponbare' Using leaves from seedlings at the three-leaf stage as material, total RNA was extracted using the TRIzol method. First-strand cDNA was synthesized using the extracted total RNA as a template via reverse transcription.

[0025] 2. Full-length gene cloning: Based on the predicted OsLRR-RLK-RI1 gene sequence from the rice genome database, specific amplification primers were designed.

[0026] The upstream primer sequence is 5'-ATGGTGAAGAGGCGGCTGTT-3', and the downstream primer sequence is 5'-CTACGTGCCCCTGTTCTTCA-3'.

[0027] Rice cDNA was used as a template for PCR amplification using high-fidelity DNA polymerase. The reaction conditions were: 98℃ pre-denaturation for 3 minutes; 98℃ denaturation for 10 seconds, 58℃ annealing for 30 seconds, and 72℃ extension for 2 minutes, for a total of 35 cycles; and a final extension at 72℃ for 10 minutes. After agarose gel electrophoresis, the PCR products were purified by gel extraction.

[0028] 3. Sequencing and Analysis: The purified PCR product was ligated into the pMD19-T vector, transformed into *E. coli* DH5α competent cells, and positive clones were screened and sequenced. Sequencing results showed that the full-length coding sequence (CDS) of the OsLRR-RLK-RI1 gene was 2832 bp (nucleotide sequence shown in SEQ ID NO: 1), encoding 943 amino acid residues (amino acid sequence shown in SEQ ID NO: 2).

[0029] Bioinformatics analysis showed that the OsLRR-RLK-RI1 protein contains a signal peptide at its N-terminus, an extracellular region containing a leucine-rich repeat (LRR) domain, a transmembrane region, and an intracellular region containing a serine / threonine kinase domain, making it a typical member of the LRR-type receptor kinase family.

[0030] Example 2: Expression analysis of OsLRR-RLK-RI1 gene in different rice tissues 1. Tissue sample collection: Take different tissues such as roots, stems, leaves, flowers and seeds of rice plants of 'Nipponbare' that have grown to the appropriate stage, and quickly place them in liquid nitrogen for freezing and preservation.

[0031] 2. RNA extraction and cDNA synthesis: Tissue samples were ground into powder, and total RNA was extracted using the TRIzol method. Using 1 μg of total RNA as a template, cDNA was synthesized using a reverse transcription kit.

[0032] 3. Real-time quantitative PCR analysis: qRT-PCR specific primers were designed based on the CDS sequence of the OsLRR-RLK-RI1 gene.

[0033] The upstream primer sequence is 5'-CTGGGTCAAGAATCGCTT-3', and the downstream primer sequence is 5'-CCCTTCCAAGTTCATACCG-3'.

[0034] Using the rice Actin gene as an internal control, among which: Its upstream primer sequence is: 5'-CTGCGGGTATCCATGAGACT-3'; The downstream primer sequence is: 5'-GGAGCAAGGCAGTGATCTTC-3'.

[0035] qRT-PCR was performed using the SYBR Green fluorescent dye assay. The reaction conditions were: 95℃ pre-denaturation for 30 seconds; 95℃ denaturation for 5 seconds; 60℃ annealing and extension for 30 seconds, for a total of 40 cycles. Three technical replicates were performed for each sample, using 23... -ΔΔCt The relative expression level of a gene can be calculated.

[0036] 4. Results: such as Figure 1 As shown, the OsLRR-RLK-RI1 gene is expressed in rice roots, stems, leaves, flowers, and seeds. Its expression level is highest in roots, about 18 times that in leaves, and its expression level in seeds, stems, and flowers is 2-4 times that in leaves.

[0037] Example 3: Construction and disease resistance identification of OsLRR-RLK-RI1 gene CRISPR / Cas9 knockout mutant 1. Target Design and Vector Construction: Using CRISPR / Cas9 gene editing technology, two target sites were designed in the exon region of the OsLRR-RLK-RI1 gene, namely: The sequence of target 1 is: 5'-CAATGATCCTTGTGCGAGATGGG-3'; The sequence of target 2 is: 5'-GGTACTCTCACACCGGCTATTGG-3'.

[0038] The target sequences were constructed into CRISPR / Cas9 expression vectors to obtain recombinant plasmids.

[0039] 2. Genetic transformation of rice: Recombinant plasmids were transformed into wild-type rice Zhonghua 11 (ZH11) callus tissue via Agrobacterium-mediated transformation. Resistant callus tissue was obtained by screening with hygromycin and further differentiated and regenerated to obtain transgenic plants.

[0040] 3. Mutant Identification: Genomic DNA was extracted from transgenic plants, and PCR amplification was performed using target-specific flanking primers. The PCR products were then sequenced. Analysis of the sequencing peaks identified lines with homozygous mutations in the OsLRR-RLK-RI1 gene. Two independent homozygous knockout lines (named...) were selected. oslrr-rlk-ri1-1 and oslrr-rlk-ri1-2 (To conduct subsequent experiments)

[0041] 4. Inoculation with rice blast fungus and identification of disease resistance: Knockout mutant plants and wild-type control plants were cultured in a greenhouse until the 4-leaf stage. The rice blast fungus (…) was inoculated using a spray method. Magnaporthe oryzae Spore suspension of highly pathogenic strain (Zhong11) (concentration 1×10⁻⁶) 5 The spores (containing 0.02% Tween-20) were evenly sprayed onto the surface of rice leaves. After inoculation, the plants were placed in a dark room at 25℃ and relative humidity >90% for 24 hours for moist cultivation, and then transferred to normal greenhouse conditions for cultivation.

[0042] 5. Disease Investigation and Statistical Analysis: Seven days after inoculation, the disease incidence of each plant was investigated. The number of lesions on each leaf was counted, and the percentage of lesion area to the total leaf area was measured. At least 15 plants were investigated for each line, and the mean and standard error were calculated. A t-test was used to analyze the significance of differences.

[0043] 6. Results: such as Figure 2 As shown, compared with wild-type control plants, OsLRR-RLK-RI1 gene knockout mutant plants showed more and larger lesions on their leaves after inoculation with rice blast fungus, and the lesion area was significantly increased. P <0.01). This result indicates that the deletion of the OsLRR-RLK-RI1 gene leads to a significant reduction in rice resistance to rice blast, meaning that OsLRR-RLK-RI1 is a positive regulator of rice blast resistance.

[0044] Example 4: Construction and disease resistance identification of plants overexpressing the OsLRR-RLK-RI1 gene 1. Overexpression vector construction: Using 'Nipponbare' rice cDNA as a template, the full-length CDS sequence of the OsLRR-RLK-RI1 gene was amplified using high-fidelity DNA polymerase. The PCR product was cloned into plant overexpression vectors such as pCAMBIA1300-35S, and placed under the control of a strong constitutive promoter (such as the CaMV 35S promoter) to construct the recombinant overexpression plasmid p35S::OsLRR-RLK-RI1.

[0045] 2. Rice genetic transformation: The recombinant overexpression plasmid was transformed into wild-type rice Zhonghua 11 (ZH11) callus tissue via Agrobacterium-mediated transformation. Resistant callus tissue was obtained by screening with hygromycin and further differentiated and regenerated to obtain transgenic plants.

[0046] 3. Identification of positive plants: Genomic DNA was extracted from transgenic plants, and PCR identification was performed using vector-specific primers and gene-specific primers. Total RNA was further extracted from positive plants, and the expression level of the OsLRR-RLK-RI1 gene was detected by qRT-PCR. Overexpression lines with significantly elevated expression levels were screened. Two independent overexpression lines (named OsLRR-RLK-RI1-OE-1 and OsLRR-RLK-RI1-OE-2) were selected for subsequent experiments.

[0047] 4. Inoculation with rice blast fungus and identification of disease resistance: Following the same method as in Example 3, overexpressing plants and wild-type control plants were sprayed with rice blast fungus, and the disease incidence was investigated 7 days after inoculation.

[0048] 5. Results: such as Figure 3As shown, compared with wild-type control plants, plants overexpressing the OsLRR-RLK-RI1 gene exhibited a significant reduction in the number and area of ​​leaf lesions after inoculation with rice blast fungus (P<0.01). This result indicates that overexpression of the OsLRR-RLK-RI1 gene can significantly enhance rice resistance to rice blast.

[0049] Example 5: Subcellular localization analysis of OsLRR-RLK-RI1 protein 1. Subcellular localization vector construction: The CDS sequence (without the stop codon) of the OsLRR-RLK-RI1 gene was cloned into the pHF-223 plant transient expression vector and fused with green fluorescent protein (GFP) to construct the recombinant plasmid pUbi::OsLRR-RLK-RI1-GFP.

[0050] 2. Protoplast transformation and observation: Protoplasts were extracted from leaves of rice variety Zhonghua 11 (ZH11), and recombinant plasmids were transformed into the protoplasts using the PEG-mediated method. After transformation, the protoplasts were incubated in the dark at 25℃ for 16-24 hours, and the GFP fluorescence signal was observed using a confocal laser scanning microscope.

[0051] 3. Results: such as Figure 4 As shown, the fluorescence signal of the OsLRR-RLK-RI1-GFP fusion protein is mainly distributed in the cell membrane of rice protoplasts, which is consistent with the membrane localization characteristics of known LRR-type receptor kinases, further confirming that OsLRR-RLK-RI1 is a receptor kinase located in the cell membrane.

[0052] Example 6: Analysis of the expression response of the OsLRR-RLK-RI1 gene induced by rice blast fungus 1. Pathogen inoculation and sampling: Rice plants of variety Zhonghua 11 (ZH11) were cultured to the 4-leaf stage. A suspension of rice blast fungus spores (concentration 1×10⁻⁶) was inoculated. 5 Spray the spores ( / mL) evenly onto the surface of rice leaves, and take leaf samples at 0, 6, 12, 24 and 48 hours (H) after inoculation. The samples were then quickly placed in liquid nitrogen for freezing and preservation.

[0053] 2. Expression analysis: Total RNA was extracted from samples at each time point and reverse transcribed into cDNA. The expression level of the OsLRR-RLK-RI1 gene at different time points of rice blast fungus infection was detected by qRT-PCR.

[0054] 3. Results: such as Figure 5As shown, compared with 0 hours (H), the expression of the OsLRR-RLK-RI1 gene began to be significantly upregulated 6 hours (H) after inoculation with rice blast fungus, reaching a peak at 12-24 hours (H), and then gradually declining. This result indicates that the expression of the OsLRR-RLK-RI1 gene is induced by rice blast fungus infection, further supporting the conclusion that it participates in the immune response against rice blast.

[0055] The above description is only a part of the embodiments of the present invention and does not limit the scope of protection of the present invention. Any equivalent device or equivalent process transformation made based on the content of the present invention specification and drawings, or direct or indirect application in other related technical fields, are similarly included within the patent protection scope of the present invention.

Claims

1. A rice LRR-type receptor kinase OsLRR-RLK-RI1, characterized in that, Its amino acid sequence is one of the following: a) Has the amino acid sequence shown in SEQ ID NO: 2; b) A variant having at least 90%, 95%, 98% or 99% sequence identity with the amino acid sequence shown in SEQ ID NO: 2, and retaining the function of regulating rice resistance to rice blast.

2. The gene encoding the rice LRR-type receptor kinase OsLRR-RLK-RI1 as described in claim 1, characterized in that: It has a nucleotide sequence as shown in SEQ ID NO:

1.

3. A rice LRR-type receptor kinase OsLRR-RLK-RI1 gene, characterized in that, Its nucleotide sequence is one of the following: a) Has a nucleotide sequence as shown in SEQ ID NO: 1; b) A variant having at least 90%, 95%, 98% or 99% sequence identity with the nucleotide sequence shown in SEQ ID NO: 1 and encoding a protein that retains the function of regulating rice resistance to rice blast.

4. A recombinant expression vector, characterized in that, It contains the gene as described in claim 2 or the rice LRR-type receptor kinase OsLRR-RLK-RI1 gene as described in claim 3.

5. A host cell, characterized in that, It comprises the recombinant expression vector as described in claim 6, or the gene as described in claim 2 or the rice LRR-type receptor kinase OsLRR-RLK-RI1 gene as described in claim 3 integrated into its genome.

6. The use of the gene as described in claim 2 or the rice LRR-type receptor kinase OsLRR-RLK-RI1 gene as described in claim 3 in regulating rice resistance to rice blast.

7. The use as described in claim 6, characterized in that, The regulation is positive regulation, which enhances rice resistance to rice blast by increasing the expression level or activity of the gene-encoded rice LRR-type receptor kinase OsLRR-RLK-RI1.

8. The application of the gene as described in claim 2 or the rice LRR-type receptor kinase OsLRR-RLK-RI1 gene as described in claim 3 in the breeding of rice varieties resistant to rice blast, characterized in that: The applications include enhancing rice resistance to rice blast by overexpressing the gene and / or increasing the expression level or activity of the gene-encoded rice LRR-type receptor kinase OsLRR-RLK-RI1.

9. A method for improving rice resistance to rice blast, characterized in that, It includes: Overexpression of the rice LRR receptor kinase OsLRR-RLK-RI1 gene in rice, or enhancement of the expression level or activity of the rice LRR receptor kinase OsLRR-RLK-RI1; the overexpression includes transforming an expression vector containing the rice LRR receptor kinase OsLRR-RLK-RI1 gene into rice cells to obtain transgenic rice plants with increased OsLRR-RLK-RI1 gene expression levels; The nucleotide sequence of the rice LRR-type receptor kinase OsLRR-RLK-RI1 gene is one of the following: a) Has a nucleotide sequence as shown in SEQ ID NO: 1; b) A variant having at least 90%, 95%, 98% or 99% sequence identity with the nucleotide sequence shown in SEQ ID NO: 1, and encoding a variant that retains the functional protein regulating rice resistance to rice blast. The amino acid sequence of the rice LRR-type receptor kinase OsLRR-RLK-RI1 is one of the following: a) Has the amino acid sequence shown in SEQ ID NO: 2; b) A variant having at least 90%, 95%, 98% or 99% sequence identity with the amino acid sequence shown in SEQ ID NO: 2, and retaining the function of regulating rice resistance to rice blast.

10. The application of the gene as described in claim 2 or the rice LRR-type receptor kinase OsLRR-RLK-RI1 gene as described in claim 3 in the preparation of transgenic plants resistant to rice blast.

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