Method for cultivating large-sized trachurus japonicus in pelagic deep sea land-sea relay

Abstract

The application discloses a Trachurus japonicus deep-sea land-sea relay large-size fry cultivation method and relates to the technical field of aquaculture.The method comprises the following steps: putting Trachurus japonicus fry in a land-based cultivation facility, cultivating the Trachurus japonicus fry into medium-size fry, sequentially grading and screening the medium-size fry and carrying out water running exercise, then transferring the medium-size fry to a deep-sea cultivation area, and putting the medium-size fry in a cultivation net cage in the deep-sea cultivation area and continuing to cultivate the medium-size fry into large-size fry.The two-segment relay cultivation mode of land-based cultivation to medium-size grading and screening and water running exercise, transfer to a deep sea and deep-sea cultivation to large size has the advantages of strong controllability of a land-based environment and excellent characteristics of a deep-sea environment, effectively solves the problems of low survival rate and unstable quality of the current Trachurus japonicus cultivation method, and realizes efficient and standardized cultivation of large-size Trachurus japonicus fry.

Description

Technical Field

[0001] This application relates to the field of aquaculture technology, and in particular to a method for cultivating large-sized oval pomfret fry in deep-sea and land-sea relay. Background Technology

[0002] The golden pomfret (also known as the oval pomfret) is an important marine aquaculture fish species in the southern coastal areas of my country, with an annual output exceeding 300,000 tons, making it the largest deep-sea aquaculture fish species in my country. With the rapid development of the deep-sea aquaculture industry, the demand for high-quality, large-sized fish species is becoming increasingly urgent.

[0003] Currently, the cultivation of large-sized oval pomfret fry in the industry mainly involves three technical models: land-based cultivation, nearshore fish raft and nearshore cage cultivation. Fry cultivated in land-based hatcheries lack intermediate rearing processes adapted to the aquaculture areas and are directly transferred to deep-sea cages for adult rearing, resulting in low survival rates. Fry cultivated in nearshore fish rafts and cages suffer from unstable quality and low survival rates due to eutrophication in near-shallow waters and frequent aquaculture diseases; these methods are no longer suitable for current large-scale production needs.

[0004] Therefore, there is an urgent need for a new method for cultivating large-sized oval pomfret fry to solve the problems of low survival rate and unstable quality in the current methods for cultivating oval pomfret fry. Summary of the Invention

[0005] The main purpose of this application is to propose a method for cultivating large-sized oval pomfret fry in deep-sea and land-sea relay, aiming to solve the problems of low survival rate and unstable quality in current oval pomfret fry cultivation methods.

[0006] To achieve the above objectives, this application proposes a method for cultivating large-sized oval pomfret fry in deep-sea and land-sea relay systems, comprising: Oval pomfret fry are released into a land-based aquaculture facility and then raised to medium-sized fingerlings. After the medium-sized fish fry are graded, screened, and trained in water, they are transferred to the deep-sea aquaculture area. The medium-sized fish fry are released into the aquaculture cages in the deep-sea aquaculture area and then further cultivated into large-sized fish fry.

[0007] In one embodiment, the step of releasing oval pomfret fry into a land-based rearing facility and raising the oval pomfret fry to medium-sized fingerlings includes: Obtain qualified oval pomfret fry; the oval pomfret fry have a size of greater than or equal to 0.4 g / ind and less than or equal to 0.6 g / ind; After cleaning, disinfecting, and filling the land-based cultivation facility with water, the oval pomfret fry are released at the first stocking density; The oval pomfret fry were cultivated in accordance with the first feeding management plan and the first water quality management plan; When the oval pomfret fry grow to a weight greater than or equal to 8 g / ind and less than or equal to 10 g / ind, the medium-sized fish species are obtained.

[0008] In one embodiment, the oval pomfret fry are at least fry that do not carry specific pathogens, and the specific pathogens include at least rhabdovirus, neuronecrosis virus, Cryptocaryon irritans, and Amylodinium oogonium; the land-based cultivation facility is set as a pond; The step of cleaning, disinfecting, and filling the land-based cultivation facility with water, and then releasing the oval pomfret fry at a first stocking density, includes: After drying the pond and exposing it to the sun for 10 to 15 days, disinfect the entire pond by spraying it with chlorinated lime. After cleaning the pond for 7 days, fill it with water. Before stocking, take a sample of the water body to be stocked, and put the same type of fish fry into the water sample and observe for 24 hours to test the water quality. After confirming through water testing that the same type of fish fry are active and feeding normally, the oval pomfret fry are released at a first stocking density of 12,000 to 20,000 fry / mu, with a stocking water temperature ≥23℃ and a temperature difference of ≤2℃ between the stocking water temperature and the transport water temperature.

[0009] In one embodiment, the first feeding management involves feeding the fish 3 to 4 times a day, with a daily feeding amount of 8% to 12% of the fish's body weight. The first water quality management plan includes: not changing the water in the early stage of stocking, only adding a small amount of water every day; after 7 days of stocking, changing 10% to 20% of the water every day; during the breeding process, adjusting the water quality by turning on the aerator, changing the water, or sprinkling water quality regulators according to the water color, water temperature, and dissolved oxygen of the pond. The first feeding management plan includes adding at least one of allicin, immunopolysaccharide, or traditional Chinese medicine preparation to the feed; And / or, the step of obtaining the medium-sized fish fry when the oval pomfret fry grow to a weight greater than or equal to 8 g / ind and less than or equal to 10 g / ind includes: When the oval pomfret fry grow to a weight greater than or equal to 8 g / ind and less than or equal to 10 g / ind, a first quality test is performed to obtain the first quality test result; the first quality test includes at least one of the following: body shape, body color, fin integrity, scale integrity, reaction speed, deformity rate, injury rate, and pathogen status; If the first quality test result is qualified, then the medium-sized fish species is obtained.

[0010] In one embodiment, the step of sequentially grading, screening, and acclimating the medium-sized fish fry before transferring them to a deep-sea aquaculture area includes: The medium-sized fish species are divided into 2 to 3 size grades according to their body weight, so that the size of fish species within the same grade remains consistent. After grading, the medium-sized fish fry were placed in a directional water flow for 30 to 60 minutes to allow them to empty their feces, reduce the amount of mucus on their bodies, and adapt to the dense environment. The medium-sized fish fry, after being exercised in the water, are transferred to the deep-sea aquaculture area at a second transport density using a live water truck and a live water boat in sequence; wherein, the live water truck and the live water boat continuously supply oxygen and maintain a set water temperature during the transfer process.

[0011] In one embodiment, the set water temperature is 23°C to 25°C, and the second transport density is 6500 ind / m³ to 9000 ind / m³. The water truck and the water boat are equipped with a circulating water filtration and purification system, which includes a mechanical filtration unit, a biological filtration unit, a protein separation unit, and an ultraviolet disinfection unit. During the transfer process, the dissolved oxygen in the transferred water is ≥5mg / L, and the ammonia nitrogen is <0.3mg / L. The water truck and the water boat use a dual oxygen supply method of pure oxygen and air oxygen, and the water is changed once every 4 hours during the transfer.

[0012] In one embodiment, the step of releasing the medium-sized fish fry into the aquaculture cages in the deep-sea aquaculture area and further cultivating the medium-sized fish fry to large-sized fish fry includes: When the current is calm, the medium-sized fish fry are released into the aquaculture cages at a second stocking density of 40 ind / m³ to 60 ind / m³. The medium-sized fish species are cultivated through a second feeding management method. The second feeding management method involves feeding the fish 2 to 3 times a day, during periods of slow or calm tides, with a daily feeding amount of 5% to 8% of the fish's body weight. The feed used is a special extruded compound feed for oval pomfret. Depending on the weather, water quality, and the fish's condition, reduce or stop feeding. When the medium-sized fish grows to a weight of 50g / ind to 125g / ind, the large-sized fish is obtained.

[0013] In one embodiment, the deep-sea aquaculture area is ≥10km offshore, with an average water depth ≥13m, a current velocity of 0.2m / s to 1.0m / s, a seawater salinity of 18 to 32, a water temperature of 23℃ to 32℃, and dissolved oxygen ≥5 mg / L; The aquaculture cage is set as an HDPE gravity cage, including an HDPE frame system, a PE netting system and an anchoring system. The mesh size of the HDPE gravity cage is 2cm to 4cm. The arrangement direction of the multiple aquaculture cages is consistent with the tidal flow direction, and the distance between adjacent aquaculture cages is ≥100 m.

[0014] In one embodiment, the step of further cultivating the medium-sized fish fry to large-sized fish fry also includes routine management steps: Inspect and replace or clean the mesh every 15 to 30 days. Cleaning methods include high-pressure water spraying or sun exposure and beating. During disease outbreak seasons, add immune enhancers to the feed before feeding; When parasitic diseases occur, freshwater immersion and / or drug immersion shall be used for treatment; wherein, the parasitic diseases include Cryptocaryon irritans infection, Amylodinosis, and Benedenia infection; When bacterial diseases occur, medicated feed is used for treatment; the bacterial diseases include streptococcal disease and bacterial enteritis.

[0015] In one embodiment, the method for cultivating large-sized oval pomfret fry in deep-sea and land-sea relay also includes: For the large-sized fish, stop feeding for 1 day, gather them together with nets, and then divide the fish. The large-sized fish fry are transported to rearing cages using live-water vessels, where they are then cultured as adult fish; the transport density of the live-water vessels is 120 ind / m³ to 140 ind / m³. A second quality test was conducted before the large-sized fish fry were hatched, and the results of the second quality test were obtained. If the second quality inspection result is qualified, the large-sized fish fry will be harvested.

[0016] The technical solution of this application first involves releasing oval pomfret fry into land-based cultivation facilities and raising them to medium-sized fingerlings. This fully utilizes the strong controllability of the land-based cultivation environment, allowing small-sized fry to complete early rapid growth and physical strengthening in a stable environment, laying a good foundation for subsequent deep-sea cultivation. Then, the medium-sized fingerlings are sequentially graded, screened, and subjected to water training before being transferred to the deep-sea aquaculture area. Grading and screening ensure the consistency of fingerling size within the same batch, avoiding growth differentiation caused by size differences during subsequent cultivation. Water training involves applying moderate exercise stress to the fingerlings through directional water flow, prompting them to empty their feces, reduce body surface mucus, and strengthen their physical condition. This physiologically prepares the fingerlings for transfer from land-based to deep-sea environments, effectively reducing stress and mortality during the transfer process. Finally, by stocking medium-sized fish fry into aquaculture cages in deep-sea aquaculture areas and continuing to cultivate them to large sizes, the excellent environmental conditions of the open deep-sea waters—clean water quality, good water exchange, and sufficient dissolved oxygen—were fully utilized. This allowed the fish fry to grow rapidly to large sizes in an environment with low disease risk. At the same time, due to the consistency between the deep-sea cultivation environment and the subsequent adult fish farming environment, the fish fry did not need to undergo a secondary environmental adaptation process after being released into the growth cages.

[0017] Overall, this application utilizes a two-stage relay cultivation method—land-based cultivation to medium size, grading and screening with water training, transfer to the deep sea, and deep-sea cultivation to large size—which leverages the advantages of the highly controllable land-based environment and the superior characteristics of the deep-sea environment. This effectively solves the problems of low survival rate and unstable quality in current deep-sea relay cultivation methods for large-sized oval pomfret, achieving efficient and standardized cultivation of large-sized oval pomfret. Attached Figure Description

[0018] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without creative effort.

[0019] Figure 1 A flowchart illustrating the first embodiment of the method for cultivating large-sized oval pomfret fry in deep-sea and land-sea relays provided in this application; Figure 2 A flowchart illustrating the second embodiment of the method for cultivating large-sized oval pomfret fry in deep-sea and land-sea relays provided in this application; Figure 3 A flowchart illustrating the third embodiment of the method for cultivating large-sized oval pomfret fry in deep-sea and land-sea relays provided in this application; Figure 4 A flowchart illustrating the fourth embodiment of the method for cultivating large-sized oval pomfret fry in deep-sea and land-sea relays provided in this application; Figure 5 A flowchart illustrating the sixth embodiment of the method for cultivating large-sized oval pomfret fry in deep-sea and land-sea relays provided in this application; Figure 6 A flowchart illustrating the seventh embodiment of the method for cultivating large-sized oval pomfret fry in deep-sea and land-sea relays provided in this application; Figure 7 A simplified flowchart illustrating the deep-sea, land-sea relay breeding method for oval pomfret provided in this application.

[0020] Explanation of icon numbers: The realization of the purpose, functional features and advantages of this application will be further explained in conjunction with the embodiments and with reference to the accompanying drawings. Detailed Implementation

[0021] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this application, and not all of the embodiments. Based on the embodiments of this application, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of this application.

[0022] It should be noted that if the embodiments of this application involve directional indicators (such as up, down, left, right, front, back, etc.), the directional indicators are only used to explain the relative positional relationship and movement of the components in a specific posture. If the specific posture changes, the directional indicators will also change accordingly.

[0023] Furthermore, if the embodiments of this application involve descriptions such as "first" or "second," these descriptions are for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined with "first" or "second" may explicitly or implicitly include at least one of those features. Additionally, the use of "and / or" or "and / or" throughout the text includes three parallel solutions. For example, "A and / or B" includes solution A, solution B, or a solution that simultaneously satisfies A and B. Furthermore, the technical solutions of the various embodiments can be combined with each other, but this must be based on the ability of those skilled in the art to implement them. When the combination of technical solutions is contradictory or impossible to implement, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection claimed in this application.

[0024] Currently, the cultivation of large-sized oval pomfret fry in the industry mainly involves three technical models: land-based cultivation, nearshore fish raft and nearshore cage cultivation. Fry cultivated in land-based hatcheries lack intermediate rearing processes adapted to the aquaculture areas and are directly transferred to deep-sea cages for adult rearing, resulting in low survival rates. Fry cultivated in nearshore fish rafts and cages suffer from unstable quality and low survival rates due to eutrophication in near-shallow waters and frequent aquaculture diseases; these methods are no longer suitable for current large-scale production needs.

[0025] Therefore, there is an urgent need for a new method for cultivating large-sized oval pomfret fry in deep-sea and land-sea relay systems to solve the problems of low survival rate and unstable quality in current oval pomfret fry cultivation methods.

[0026] To address the aforementioned issues, this application proposes a method for cultivating large-sized oval pomfret fry in deep-sea and land-sea relay systems.

[0027] Please see Figure 1 In the first embodiment of this application, the method for cultivating large-sized oval pomfret fry in deep-sea and land-sea relay includes steps S10 to S30: Step S10: Release oval pomfret fry into the land-based breeding facility and raise the oval pomfret fry to medium-sized fish fry; It should be noted that land-based culture facilities refer to artificial facilities built on land for the cultivation of fish fry. Their water source is seawater or brackish water, and a suitable growth environment is provided for the fry through artificial control and management. Land-based culture facilities can take various forms, including but not limited to earthen ponds and cement ponds. Their common characteristics are that they are all located on land and have water intake and drainage systems and water quality management capabilities. Medium-sized fry refer to fish that have grown to a certain size after the land-based culture stage and possess strong environmental adaptability. Fish of this size have the physiological basis to be transferred from the land-based environment to the deep-sea environment for further cultivation.

[0028] In step S10, the implementing entity first releases oval pomfret fry into the land-based rearing facility. The implementing entity determines the quantity and size of the fry to be released based on the fry supply and the rearing plan. Before release, the implementing entity needs to perform necessary preparatory work on the land-based rearing facility, including cleaning, disinfection, water filling, and water quality adjustment, to ensure a suitable environment for the fry's survival. The implementing entity selects an appropriate time window for release, avoiding adverse conditions such as high temperatures, strong light, and severe weather. After release, the implementing entity conducts daily rearing management of the fry according to the established feeding and water quality management plan, including feeding the fry at regular intervals and in fixed quantities, monitoring and adjusting water quality parameters, and inspecting the activity status of the fish school. During the rearing process, the implementing entity periodically samples and measures the weight and length of the fry to assess their growth rate, and adjusts the feeding amount and management measures as needed based on the growth situation until the fry grow to a medium-sized fingerling.

[0029] In one example, the implementing entity released quarantined oval pomfret fry into a 0.5-hectare earthen pond with an average depth of 1.8 meters. The fry weighed 0.5 grams each, and the stocking density was 150,000 fry per acre. Before stocking, the implementing entity had completed the dredging, disinfection, and water filling of the pond. After stocking, the implementing entity fed the fry three times a day, with a daily feed amount of 10% of the fry's body weight, and turned on the aerator and changed the water as needed based on water quality monitoring results. After about 30 days of cultivation, the fry grew to 9 grams each, reaching the standard for medium-sized fish.

[0030] In another example, the operator stocked oval pomfret fry in a cement pond at a density of 180,000 fry per acre, with each fry weighing 0.4 grams. The cement pond was equipped with an independent water inlet and outlet system and aeration equipment. The operator maintained water quality stability by regularly changing the water and turning on the aerator. After about 35 days of cultivation, the fry grew to 8 grams per fry, reaching the standard for medium-sized fish.

[0031] Step S20: After grading, screening and water training of the medium-sized fish fry, the medium-sized fish fry are transferred to the deep-sea aquaculture area. It should be noted that grading and screening refers to the process of dividing fish into different size grades based on differences in body size among individuals using screening equipment. The purpose of grading and screening is to divide fish of varying sizes into several groups with relatively uniform sizes, so as to avoid uneven feeding competition and growth differentiation problems caused by excessive differences in individual size during subsequent breeding. Running-in training refers to a physical strengthening training method in which fish are placed in a directional water flow and made to swim against the current. During running-in training, the fish need to swim continuously to resist the thrust of the water flow, which fully exercises the cardiopulmonary and muscular systems. At the same time, moderate exercise stress can activate the fish's stress adaptation mechanisms. Running-in training also helps the fish empty residual feces from their digestive tract and reduces mucus secretion from their body surface, physiologically preparing the fish for intensive transport.

[0032] In step S20, the executing entity first grades and sorts the fish fry that have reached the medium size. After harvesting the fish from the land-based cultivation facility, the executing entity sends the fry to a grading device, where they are divided into two to three size grades based on body weight using grading bars or screens of different sizes. After grading, the executing entity gathers the fry of the same size grade together for water conditioning. The executing entity creates a directional water flow in the conditioning tank by activating a waterwheel or pump, placing the fry in the water flow and causing them to swim against the current. During the water conditioning process, the executing entity continuously observes the activity status of the fry to ensure that no abnormalities occur. After the water conditioning is completed, the executing entity loads the fry into a live water truck, activates the circulating water system and oxygenation system of the live water truck, and transports the fry from the land-based cultivation facility area to the deep-sea aquaculture area. During transportation, the executing entity continuously monitors key parameters such as the temperature and dissolved oxygen of the transport water to ensure that the fry are in good physiological condition during transportation.

[0033] In one example, the operator grading medium-sized fish fry into two grades, with the body weight difference within each grade not exceeding 10%. After grading, the operator places the fish of the same grade in a training tank, activates a waterwheel to create a directional water flow at a velocity of 0.5 m / s, and makes the fish swim against the current for 45 minutes. After the training period, the operator loads the fish into a live-water truck equipped with a circulating water filtration and purification system and a temperature control system. The transport water temperature is set at 24 degrees Celsius, and the transport density is 7500 fish / cubic meter. The live-water truck continuously oxygenates the fish, transporting them from the land-based rearing farm to the deep-sea cage aquaculture base dock, changing the water once en route. The entire process takes approximately 5 hours. The fish are then transferred to a live-water vessel, which transports them to the deep-sea aquaculture area.

[0034] Step S30: The medium-sized fish fry are released into the aquaculture cages in the deep-sea aquaculture area, and the medium-sized fish fry are further cultivated into large-sized fish fry.

[0035] It should be noted that deep-sea aquaculture areas refer to open marine aquaculture areas that are far from the shore, have greater water depth, and better water exchange, as opposed to nearshore waters and land-based aquaculture environments. Deep-sea aquaculture areas typically feature clean water, sufficient dissolved oxygen, and suitable current rates, which are conducive to the rapid growth of fish fry and disease control. Large-sized fish fry refer to fish that have grown to a suitable size after two stages of deep-sea cage culture and are ready for release into adult rearing cages.

[0036] In step S30, after transporting medium-sized fish fry to the deep-sea aquaculture area, the implementing entity releases the fry into pre-deployed aquaculture cages during a period of calm currents. During release, the implementing entity controls the stocking density to ensure the fry have sufficient space to grow within the cages. After release, the implementing entity manages the fry according to a predetermined feeding plan. The implementing entity adjusts the feeding amount based on the fry's feeding behavior and growth rate, regularly inspects the cage structure for safety and the fish's health, checks the netting for any attached debris, and cleans or replaces the netting as needed. During disease outbreaks, the implementing entity takes appropriate preventative measures. After a period of cultivation, the medium-sized fry grow into large-sized fry.

[0037] In one example, the implementer stocked medium-sized fish fry in HDPE gravity cages in a deep-sea aquaculture area at a density of 50 fish per cubic meter. The implementer fed the fish twice daily, using specialized extruded compound feed during periods of low tide, at a daily rate of 6% of the fish's body weight. After approximately 60 days of rearing, the fish fry grew from 9 grams per fish to 80 grams per fish, reaching the standard for large-sized fry.

[0038] In another example, the implementing entity stocked medium-sized fish fry in aquaculture cages deployed in deep-sea aquaculture areas at a stocking density of 40 fish per cubic meter. The implementing entity fed the fish three times a day, with a daily feed amount of 7% of the fish's body weight. After approximately 45 days of cultivation, the fish fry grew from 10 grams per fish to 100 grams per fish, reaching the standard for large-sized fish fry.

[0039] This embodiment provides a method for cultivating large-sized oval pomfret fry in deep-sea environments using a land-sea relay system. The technical solution of this application first involves releasing oval pomfret fry into a land-based cultivation facility and cultivating them to medium-sized fry. This fully utilizes the strong controllability of the land-based cultivation environment, allowing small-sized fry to complete early rapid growth and physical strengthening in a stable environment, laying a good foundation for subsequent deep-sea cultivation. Then, the medium-sized fry are sequentially graded, screened, and subjected to water-cooling training before being transferred to the deep-sea aquaculture area. Grading and screening ensure the consistency of fry size within the same batch, avoiding growth differentiation due to size differences during subsequent cultivation. Water-cooling training uses directional water flow to apply moderate exercise stress to the fry, prompting them to empty their feces, reduce body mucus, and strengthen their constitution. This physiologically prepares the fry for transfer from land-based to deep-sea conditions, effectively reducing stress and mortality during the transfer process. Finally, by stocking medium-sized fish fry into aquaculture cages in deep-sea aquaculture areas and continuing to cultivate them to large sizes, the excellent environmental conditions of the open deep-sea waters—clean water quality, good water exchange, and sufficient dissolved oxygen—were fully utilized. This allowed the fish fry to grow rapidly to large sizes in an environment with low disease risk. At the same time, due to the consistency between the deep-sea cultivation environment and the subsequent adult fish farming environment, the fish fry did not need to undergo a secondary environmental adaptation process after being released into the growth cages.

[0040] Overall, this application utilizes a two-stage relay cultivation method—land-based cultivation to medium size, grading and screening with water training, transfer to the deep sea, and deep-sea cultivation to large size—which leverages the advantages of the highly controllable land-based environment and the superior characteristics of the deep-sea environment. This effectively solves the problems of low survival rate and unstable quality in current deep-sea relay cultivation methods for large-sized oval pomfret, achieving efficient and standardized cultivation of large-sized oval pomfret.

[0041] Based on the first embodiment of this application, in the second embodiment of this application, the content that is the same as or similar to the first embodiment described above can be referred to the above description, and will not be repeated hereafter. Based on this, please refer to... Figure 2 Step S10 includes steps S11 to S14: Step S11: Obtain qualified oval pomfret fry; the oval pomfret fry have a size of greater than or equal to 0.4 g / ind and less than or equal to 0.6 g / ind; It should be noted that "quarantine qualified" means that the fish fry have passed inspection by a legally authorized quarantine agency and no relevant disease pathogens have been detected, or that they meet the national standards for fish fry quarantine. The size of oval pomfret fry is expressed as the body weight of each individual, with a size range of 0.4 grams / fish to 0.6 grams / fish corresponding to the suitable stocking size for oval pomfret larvae to juvenile stages. At this size, the fry have developed independent feeding ability and a certain degree of environmental adaptability, making them suitable for release into land-based rearing facilities for further cultivation.

[0042] In this context, g / ind refers to grams per fish, and ind is an abbreviation for individual, used to represent the number of fish species. For example, 3ind means there are 3 juvenile oval pomfret.

[0043] In step S11, the implementing entity obtains oval pomfret fry from a hatchery with an aquatic seed production license. Upon receiving the fry, the implementing entity verifies the hatchery's production license, quarantine certificate, and fry origin records. The implementing entity conducts random sampling inspections of the fry to confirm that the fry's size is within the range of 0.4 g / fish to 0.6 g / fish, and that the fry are healthy, uniform in size, vigorous, and free from injury or deformity. Any batch of fry that does not meet the quality requirements is rejected by the implementing entity.

[0044] In one example, the implementing entity obtained a batch of oval pomfret fry from a hatchery with an aquatic seed production license. The fry had an average size of 0.5 grams per fish, and the quarantine certificate showed that no specific pathogens such as rhabdovirus or nerve necrosis virus were detected. The implementing entity conducted sampling inspections and confirmed that the fry were healthy, uniform in size, and vigorous, and then accepted them.

[0045] In another example, the implementing entity obtains a batch of oval pomfret fry with an average size of 0.55 grams per fish from a hatchery. This batch of fry has passed quarantine and has an intact body surface without deformities. The implementing entity accepts this batch of fry for subsequent cultivation.

[0046] Step S12: After cleaning, disinfecting and filling the land-based cultivation facility with water, release the oval pomfret fry at the first stocking density; It should be noted that cleaning and disinfection refers to the process of removing residual organic matter and pathogenic microorganisms from land-based cultivation facilities using physical or chemical methods. The purpose of cleaning and disinfection is to provide a clean, pathogen-free growth environment for the fish fry to be released, reducing the risk of disease. Water injection refers to the process of introducing fresh seawater or brackish water into the cleaned and disinfected cultivation facilities. After water injection, the aquatic environment becomes the growth medium for the fish fry. The initial stocking density refers to the initial stocking density set during the land-based cultivation stage. This density needs to take into account the carrying capacity of the cultivation facilities, the growth needs of the fish fry, and the yield per unit area.

[0047] In step S12, the implementing entity completes the cleaning and disinfection of the land-based cultivation facility before the fish fry arrive. The implementing entity first drains the residual water from the facility, removes excess silt and sediment from the bottom, and then thoroughly disinfects the facility with a disinfectant. After disinfection, the implementing entity drains the disinfectant water, exposes it to sunlight for several days, and then introduces fresh seawater or brackish water. After water injection, the implementing entity tests the water quality. Once the water quality indicators are confirmed to meet the aquaculture requirements, the fish fry are released into the cultivation facility at the initial stocking density.

[0048] In one example, the implementing entity completed the pond cleaning and disinfection work 7 days before the arrival of the fish fry. The entity drained the pond, removed the bottom silt down to a thickness of approximately 20 cm, and left the pond to dry in the sun for 12 days. After drying, the entity disinfected the entire pond by spraying chlorinated lime at a rate of 15 kg per 666.7 square meters, dissolving it in water and spreading it evenly throughout the pond. Seven days after cleaning, the entity filled the pond with water to an average depth of 1.8 meters. After filling, the entity released fish fry at a stocking density of 15,000 fry per acre.

[0049] In another example, the implementing entity used a cement pond as a land-based cultivation facility. The cleaning and disinfection method involved spraying the entire pond with a potassium permanganate solution, rinsing it thoroughly with clean water after 24 hours, and then filling it with water. After filling with water, the implementing entity released fish fry at a first stocking density of 18,000 fish per acre.

[0050] Step S13: Cultivate the oval pomfret fry according to the first feeding management plan and the first water quality management plan; It should be noted that the first feeding management plan refers to the feeding operation specifications formulated for oval pomfret fry during the land-based rearing stage, including elements such as feeding frequency, feeding amount, feed type, and feeding method. The first water quality management plan refers to the water quality control operation specifications formulated to maintain suitable water quality in the rearing water body during the land-based rearing stage, including elements such as water exchange frequency, water exchange volume, aeration method, and water quality adjustment measures.

[0051] In step S13, the implementing entity feeds the fish fry according to the pre-established first feeding management plan. The implementing entity adds feed to the rearing facility at a fixed time each day, with the amount determined based on the total weight of the fish and their feeding behavior. During feeding, the implementing entity observes the feeding activity of the fish, using the completion of feeding within a specified time as a criterion for determining the appropriate feeding amount. Simultaneously, the implementing entity performs daily maintenance on the rearing water according to the first water quality management plan. The implementing entity monitors key water quality indicators such as temperature, dissolved oxygen, pH, salinity, and ammonia nitrogen daily, and takes corresponding water quality adjustment measures based on the monitoring results, including water changes, turning on aerators, or applying water quality regulators.

[0052] In one example, the implementing entity feeds the fish four times a day according to the first feeding management plan, at 6:00 AM, 10:00 AM, 2:00 PM, and 6:00 PM, with a daily feed amount of 10% of the fish's body weight. The feed used is a specially formulated feed for oval pomfret. Following the first water quality management plan, the implementing entity does not change the water during the initial stocking period, only adding a small amount of water daily to compensate for evaporation losses. After seven days of stocking, 15% of the water is changed daily. The implementing entity tests the dissolved oxygen and temperature of the water twice a day, once in the morning and once in the afternoon, and turns on the aerator as needed based on the test results.

[0053] In another example, the implementer feeds the fish three times a day, with a daily feed amount of 8% of the fish's body weight. No water changes are made in the early stages of stocking, and 20% of the water is changed daily after 7 days of stocking.

[0054] Step S14: When the oval pomfret fry grow to a weight greater than or equal to 8 g / ind and less than or equal to 10 g / ind, the medium-sized fish species are obtained.

[0055] In step S14, the implementing entity periodically samples and measures the weight of the fish fry during the cultivation process to monitor their growth progress. Every 5 to 7 days, the implementing entity randomly collects a certain number of fish fry from the cultivation facility for weighing and body length measurement, and records the growth data. When the sampling measurement results show that the average weight of the fish population reaches the range of 8 to 10 grams per fish, the implementing entity confirms that the fish fry have grown to the medium-sized fry stage and can enter the next stage of transfer and deep-sea cultivation.

[0056] In one example, the implementing entity sampled and measured the weight of the fry every 5 days during the rearing period. At the initial stocking, the average weight of the fry was 0.5 grams per fish. After 20 days of rearing, the sampling measurement showed that the average weight reached 9 grams per fish, and the implementing entity confirmed that the fry had grown to a medium-sized fish.

[0057] In another example, the implementing entity sampled and measured every 7 days. After 25 days of cultivation, the average weight of the fry reached 8.5 grams per fish, and the implementing entity confirmed that the fry met the standard for medium-sized fish.

[0058] In this embodiment, this application provides a specific implementation plan for the land-based cultivation stage of the deep-sea land-sea relay method for cultivating large-sized oval pomfret fry. First, by obtaining oval pomfret fry with a size of 0.4g / fish to 0.6g / fish and passing quarantine, the uniformity of fry quality and health status upon entering the pond are ensured from the source, avoiding the problem of inconsistent fry origin and quality leading to a lack of quality assurance at the start of cultivation. Then, after cleaning, disinfecting, and filling the land-based cultivation facilities with water, fry are released at a first stocking density, establishing standardized facility preparation and stocking operation procedures, providing a clean and safe initial growth environment for the fry. Finally, by cultivating the fry according to the first feeding management plan and the first water quality management plan, standardized and parameterized operation of feeding management and water quality control is achieved, ensuring that the fry receive sufficient nutrition and good water quality under stable environmental conditions, promoting rapid and healthy growth. Finally, by identifying medium-sized fish fry when they grew to 8-10 grams per fry, a clear stage transition standard was established, ensuring that the fish fry entering the next breeding stage were of uniform size and robust health. Overall, this embodiment formed a standardized operating procedure for the land-based breeding stage, providing uniformly sized and reliable medium-sized fish fry for subsequent transfer and deep-sea breeding.

[0059] In one optional implementation, the oval pomfret fry are at least fry that do not carry specific pathogens, and the specific pathogens include at least rhabdovirus, neuronecrosis virus, Cryptocaryon irritans, and Amylodinium oogonium; the land-based cultivation facility is set up as a pond; Step S12 includes steps S121 to S123: Step S121: After drying the pond and exposing it to the sun for 10 to 15 days, disinfect the entire pond by spraying it with chlorinated lime. After cleaning the pond for 7 days, add water. It should be noted that pond drying and sun exposure refers to the process of draining the water from a pond and exposing the pond bottom to sunlight for drying. This process utilizes the ultraviolet rays in sunlight to naturally disinfect the pond bottom, while simultaneously oxidizing and decomposing organic matter, reducing the breeding ground for pathogenic microorganisms. Chlorinated lime is a commonly used disinfectant in aquaculture; its main component is calcium hypochlorite, which releases highly oxidizing hypochlorous acid upon contact with water, capable of killing various pathogenic microorganisms in the pond sediment and water.

[0060] In step S121, the implementing entity thoroughly disinfects the pond before stocking the fish fry. First, the implementing entity drains the water from the pond and removes excess silt from the bottom, maintaining a bottom mud thickness of approximately 20 cm. Then, the implementing entity keeps the pond dry, allowing the pond bottom to be naturally exposed to sunlight for 10 to 15 days. During this exposure, the implementing entity turns over the surface mud to ensure all layers of mud receive sufficient sunlight. After the exposure, the implementing entity disinfects using chlorinated lime, dissolving the lime in water and evenly spreading it throughout the pond. After disinfection, the implementing entity keeps the pond stagnant for 7 days to allow the disinfectant to fully take effect before filling the pond with water.

[0061] In one example, the implementing entity begins pond cleaning 15 days before stocking the fish fry. After draining the pond, excess silt is removed, leaving a bottom mud thickness of approximately 20 centimeters. After the pond is dried and exposed to sunlight for 12 days, the implementing entity uses 15 kilograms of chlorinated lime per 666.7 square meters, dissolving the lime in water and evenly spreading it throughout the pond. Seven days after cleaning, the implementing entity fills the pond with water to an average depth of 1.8 meters.

[0062] In another example, the main body of the project dried the pond and exposed it to the sun for 10 days, then disinfected it with chlorinated lime at a rate of 20 kg of chlorinated lime per 666.7 square meters. The pond was then filled with water 7 days after cleaning.

[0063] Step S122: Before stocking, take a sample of the water body to be stocked, and put the same type of fish fry into the water sample and observe for 24 hours to test the water quality. It should be noted that "water testing" refers to a preliminary testing method where a small number of the same species of fry are sampled from the rearing water before the formal release of fish fry for short-term observation. The purpose of water testing is to verify whether the water quality of the rearing water is suitable for the survival of the fish fry, and to avoid mass mortality of the fry after release due to water quality problems.

[0064] In step S122, the executing entity conducts a water test operation after completing the water filling and before the formal stocking. The executing entity takes a portion of the water sample from the rearing pond and places it into a test container. Then, a small number of fish fry of the same species as the fish fry to be stocked are placed in the test container, and the activity and feeding of these fish fry are observed over 24 hours.

[0065] In one example, after filling the pond with water, the operator took a bucket of water sample and placed 10 oval pomfret fry of the same species as the batch to be released into the bucket, observing them for 24 hours. After 24 hours, the fry were active and feeding well, and the operator confirmed that the water test was successful.

[0066] Step S123: After confirming through water testing that the same type of fish fry are active and feeding normally, release the oval pomfret fry at a first stocking density; the first stocking density is 12,000 to 20,000 fry / mu, the stocking water temperature is ≥23℃, and the temperature difference between the stocking water temperature and the transport water temperature is ≤2℃.

[0067] In step S123, after confirming the successful water test, the implementing entity begins the formal stocking of fish fry. The implementing entity chooses to stock the fry in the morning or evening to avoid adverse weather conditions such as high temperatures, strong sunlight, and rain. Before stocking, the implementing entity measures the water temperature in the rearing pond and simultaneously measures the water temperature in the transport container, ensuring that the temperature difference between the two does not exceed 2 degrees Celsius. The implementing entity slowly transfers the fish fry from the transport container to the rearing pond, controlling the stocking density to be between 12,000 and 20,000 fry per acre.

[0068] In one example, after a successful trial run, the implementing entity chose to release the fish fry at 6:00 AM. Before release, the pond water temperature was measured at 24 degrees Celsius, while the transport water temperature was 23.5 degrees Celsius, a temperature difference of 0.5 degrees Celsius. The implementing entity released the fish fry into the pond at a density of 15,000 fry per acre.

[0069] In another example, the implementing entity chose to release the fish at 5 pm, with a pond water temperature of 25 degrees Celsius, a transport water temperature of 24 degrees Celsius, a temperature difference of 1 degree Celsius, and a stocking density of 18,000 fish per acre.

[0070] In this embodiment, this application provides a specific implementation plan for pond preparation and fry stocking during the land-based cultivation stage. First, the pond is dried and exposed to sunlight for 10 to 15 days, then disinfected by spraying the entire pond with chlorinated lime. After 7 days of pond cleaning, water is added, establishing a thorough pond cleaning and disinfection procedure. This effectively kills pathogenic microorganisms in the pond bottom mud and water, providing a clean initial growth environment for the fry. Then, a water quality safety verification step is established by taking water samples from the intended stocking area and placing the same type of fry in the samples for 24 hours of observation. This effectively avoids the risk of mass fry mortality due to water quality issues after stocking. Finally, after confirming the water quality through the test, fry are stocked at a density of 12,000 to 20,000 fry per acre. The stocking water temperature is strictly controlled to be no lower than 23 degrees Celsius, and the temperature difference between the stocking water and the transport water temperature is no more than 2 degrees Celsius. This ensures that the stocking density is within a reasonable range to balance farming efficiency and fry growth space, while temperature control reduces stress caused by sudden temperature changes in the fry. Overall, this embodiment, through pond disinfection, water testing and verification, and temperature-controlled stocking, lays a solid foundation for the high survival rate and rapid recovery of feeding of fish fry after they are introduced into the pond.

[0071] As an optional implementation, the first feeding management involves feeding the fish 3 to 4 times a day, with a daily feeding amount of 8% to 12% of the fish's body weight. The first water quality management plan includes: not changing the water in the early stage of stocking, only adding a small amount of water every day; after 7 days of stocking, changing 10% to 20% of the water every day; during the breeding process, adjusting the water quality by turning on the aerator, changing the water, or sprinkling water quality regulators according to the water color, water temperature, and dissolved oxygen of the pond. The first feeding management plan includes adding at least one of allicin, immunopolysaccharide, or traditional Chinese medicine preparation to the feed; And / or, step S14 includes steps S141~S142: Step S141: When the oval pomfret fry grow to a weight greater than or equal to 8 g / ind and less than or equal to 10 g / ind, a first quality test is performed to obtain the first quality test result; the first quality test includes at least one of the following: body shape, body color, fin integrity, scale integrity, physical condition, reaction speed, deformity rate, injury rate, and pathogen status; It should be noted that the first quality inspection refers to a comprehensive assessment of the fish population's quality when the fry reach the medium-sized fingerling stage. This quality inspection involves examining multiple dimensions, including the fry's appearance, health status, and pathogen carriage, to determine if the fish population meets the hatching standards. The deformity rate refers to the proportion of individuals in the population with developmental abnormalities such as spinal curvature or mouth deformities. The injury rate refers to the proportion of individuals in the population with external injuries, broken fins, or other mechanical damage. Pathogen status refers to whether the fish population carries specific pathogens.

[0072] In step S141, the executing entity conducts quality checks on the fish population when the fry grow to 8-10 grams per fish. The executing entity randomly selects a certain number of fry samples from the rearing pond and tests each sample individually. The tests include checking for normal body shape, normal body color, intact fins, intact scales, robust constitution, and sensitive reactions. Simultaneously, the deformity rate and injury rate are calculated, and samples are taken for pathogen detection.

[0073] In one example, the implementing entity randomly selected 100 fry from a medium-sized fish population for quality testing. The test results showed that all fry were of normal body shape and color, with intact fins and scales, robust constitution, and responsiveness. The deformity rate was 0.5%, the injury rate was 1.2%, and no neuronecrosis virus, Cryptocaryon irritans, or Amylodinium oogonium were detected in the pathogen test. The first quality test result was qualified.

[0074] In another example, the implementing entity randomly selected 80 fish fry for testing. The test results showed that the deformity rate was 0.8% and the injury rate was 2.5%, both of which did not exceed the qualified standards, and the pathogen test results were also negative.

[0075] Step S142: If the first quality inspection result is qualified, then the medium-sized fish species is obtained.

[0076] In step S142, the executing entity makes a judgment based on the results of the first quality inspection. If all the test indicators are within the qualified standard range, that is, the deformity rate does not exceed 1%, the injury rate does not exceed 3%, and no specific pathogens are detected, then the batch of fish is confirmed to have reached the standard for medium-sized fish fry and can enter the next stage of transportation and deep-sea cultivation.

[0077] In one example, after the implementing entity confirms that the first quality test result is qualified, the batch of fish is marked as qualified medium-sized fish species, ready to enter the grading, screening and water training stage.

[0078] In another example, if the malformation rate is found to be more than 1% during the main body detection, the batch of fish will be screened to remove malformed individuals and then tested again until it is qualified before it can enter the next stage.

[0079] In this embodiment, this application provides a specific implementation plan for feeding management, water quality management, and hatching quality testing during the land-based rearing stage. Firstly, a primary feeding management plan, involving feeding 3 to 4 times daily at a rate of 8% to 12% of the fish's body weight, adapts to the physiological characteristics of fish fry during the rapid growth stage, given their high metabolic rate, limited stomach capacity, and need for frequent small meals, ensuring sufficient nutritional supply during their rapid growth period. Secondly, a primary water quality management plan, involving no water changes initially, only small daily additions, and subsequent daily water changes of 10% to 20% after 7 days, maintains relative water stability and reduces stress in the early stages of fry introduction. After the fry adapt, regular water changes maintain good water quality conditions. Simultaneously, adjustments such as aeration, water changes, or the application of water quality regulators are flexibly selected based on water color, temperature, and dissolved oxygen levels, achieving precise and adaptable water quality management. Thirdly, by adding at least one of allicin, immunopolysaccharides, or traditional Chinese medicine preparations to the feed, these immune-enhancing substances are utilized to improve the non-specific immunity and disease resistance of the fish fry. Finally, when the fry grow to medium size, a first quality test is conducted covering multiple dimensions, including body shape, body color, fin integrity, scale integrity, physical condition, reaction speed, deformity rate, injury rate, and pathogen status. Only after passing the test can the medium-sized fish be confirmed. This establishes a strict quality control standard for fry release, ensuring that the fish entering the next breeding stage are of reliable quality, uniform size, and healthy and disease-free.

[0080] Based on the first embodiment of this application, in the third embodiment of this application, the content that is the same as or similar to that in the first embodiment can be referred to the above description, and will not be repeated hereafter. Based on this, please refer to... Figure 3 Step S20 includes steps S21 to S23: Step S21: Divide the medium-sized fish species into 2 to 3 size grades according to their body weight, so that the size of fish species within the same grade remains consistent. It should be noted that grading by body weight refers to the process of dividing a fish population into several size grades according to the size of individual fish. The purpose of grading is to homogenize the size of the fish population, which is originally of inconsistent size, so as to avoid the growth differentiation problem caused by excessive differences in individual size during subsequent breeding, which leads to strong fish competing for food and weak fish not getting enough food. It also facilitates precise control of transport density.

[0081] In step S21, after the medium-sized fish fry are harvested from the land-based rearing facility, they are immediately sent to a grading device for grading. Based on the fish fry's body weight distribution, the grading device's grating spacing is set to two to three levels. The fish fry are then divided into corresponding size grades after passing through the gratings with different spacing, keeping the body weight difference within the same grade within a small range.

[0082] In one example, the operator feeds medium-sized fish into a grading sieve for grading. The grading sieve has three size grades: Grade 1 is 8 to 9 grams per fish, Grade 2 is 9 to 10 grams per fish, and Grade 3 is over 10 grams per fish. After grading, the size difference of fish within the same grade does not exceed 10%.

[0083] In another example, the implementing entity divides medium-sized fish into two size grades: the first grade is 8 grams / fish to 9.5 grams / fish, and the second grade is 9.5 grams / fish to 11 grams / fish.

[0084] Step S22: Place the graded medium-sized fish in a directional water flow for 30 to 60 minutes to exercise them, so that the fish can empty their feces, reduce the amount of mucus on their body surface, and adapt to the dense environment. It should be noted that directional water flow refers to water flow with a fixed direction created in the running water training pool by equipment such as waterwheels and pumps. The running water training time should be controlled within the range of 30 to 60 minutes. Too short a time will not achieve the training effect, while too long a time may lead to excessive exhaustion of the fish. The physiological effects of running water training include three aspects: first, it promotes the emptying of digestive tract contents, reducing excrement pollution during transportation; second, it reduces the secretion of mucus on the body surface, reducing water quality deterioration caused by mucus shedding during transportation; and third, it forces the fish to adapt to dense environments through moderate exercise stress, enhancing their tolerance to subsequent transportation stress.

[0085] In step S22, the executing entity transfers the medium-sized fish of the same grade to a training tank. The executing entity starts a waterwheel or pump to create a directional water flow in the training tank, causing the fish to swim against the current. During the training process, the executing entity continuously observes the fish's activity to ensure no abnormalities occur. After the training is complete, the executing entity removes the fish from the training tank, preparing them for loading into transport equipment.

[0086] In one example, the operator placed medium-sized, graded fish fry in a training pool and activated a waterwheel to create a directional water flow at a speed of 0.3 meters per second. The fish fry swam against the current for 45 minutes. During the training process, the fish fry exhibited normal activity and no abnormalities were observed. After the training, the fish fry had emptied their feces, and the amount of mucus on their bodies had significantly decreased.

[0087] In another example, the implementer placed medium-sized fish in a running water training pool for 30 minutes for training, with a directional water flow velocity of 0.4 m / s.

[0088] Step S23: The medium-sized fish fry that have undergone water training are transferred to the deep-sea aquaculture area at a second transport density using a live water truck and a live water boat in sequence; wherein, the live water truck and the live water boat continuously supply oxygen and maintain a set water temperature during the transfer process.

[0089] It should be noted that "live-water truck" refers to a specialized live-fish transport vehicle equipped with a live-fish hold, a water circulation system, and an oxygenation system, suitable for short to medium-distance land transport. "Live-water boat" refers to a live-fish transport vessel equipped with a live-fish hold and an oxygenation system, suitable for sea transport. The second transport density refers to the number of fish fry loaded per unit volume of water during transport; this density needs to balance transport efficiency and fish survival rate. Continuous oxygenation refers to continuously replenishing the transport water with oxygen through oxygenation equipment throughout the transport process to maintain dissolved oxygen levels at a suitable level. Maintaining a set water temperature refers to controlling the temperature of the transport water within a set range through a temperature control system to reduce the metabolic rate and stress response of the fish fry.

[0090] In step S23, the executing entity loads the fish fry that have completed their water conditioning into the live fish hold of the live water truck, activates the truck's circulating water system and oxygenation system, and transports the fish fry from the land-based cultivation facility to the dock. Upon arrival at the dock, the executing entity transfers the fish fry from the live water truck to the live water hold of the live water vessel, which then transports the fish fry to the deep-sea aquaculture area. During the transportation process by the live water truck and the live water vessel, the executing entity continuously monitors the temperature and dissolved oxygen of the transport water to ensure that the temperature is maintained within the set range and the dissolved oxygen does not fall below the minimum requirement.

[0091] In one example, the operator loads medium-sized fish fry that have completed their water training into a live-water tanker. The live-water tanker has a 10-cubic-meter live-fish compartment, holding 75,000 fish fry at a density of 7,500 fry per cubic meter, with the transport water temperature set at 24 degrees Celsius. The live-water tanker continuously oxygenates the fish fry, transporting them from the land-based rearing farm to the dock, a journey that takes 1.5 hours. Upon arrival at the dock, the operator transfers the fish fry to a live-water vessel. The live-water vessel has a 100-cubic-meter live-water compartment, holding 750,000 fish fry at a density of 7,500 fry per cubic meter, with the transport water temperature maintained at 24 degrees Celsius. The live-water vessel then transports the fish fry to a deep-sea aquaculture area 15 kilometers offshore, a journey that takes 3.5 hours.

[0092] In another example, the executor loads the fish fry into a live-water truck at a transport density of 6,500 fish per cubic meter, with the transport water temperature set at 23.5 degrees Celsius. Upon arrival at the dock, the fish are transferred to a live-water boat, which also has a transport density of 6,500 fish per cubic meter and maintains a transport water temperature of 23.5 degrees Celsius.

[0093] In this embodiment, this application provides a specific implementation plan for the grading and screening, water training, and transportation by live-water trucks and boats during the transfer and connection phase. First, medium-sized fish fry are divided into two to three size grades based on body weight, ensuring consistency in size within each grade. This effectively avoids uneven feeding competition and growth differentiation caused by excessive size differences among individuals during subsequent deep-sea cultivation, laying the foundation for uniform growth of the fish population during the deep-sea cultivation stage. Then, the graded medium-sized fish fry are placed in a directional water flow for 30 to 60 minutes for water training. This allows the fish to empty their feces, reduce body surface mucus, and adapt to a dense environment under moderate exercise stress, physiologically preparing them for transfer and effectively enhancing their tolerance to subsequent transportation stress. Finally, the water-trained medium-sized fish fry are transferred to the deep-sea aquaculture area using live-water trucks and boats, with continuous oxygenation and maintenance of the set water temperature throughout the process. This establishes a seamless transfer and connection system from land-based to deep-sea, ensuring that the fish fry remain in a suitable aquatic environment throughout the entire transfer process. Overall, this embodiment organically combines three processes—grading and screening, water training, and temperature-controlled live water transportation—to form a complete technical closed loop from physical preparation to size uniformity and then to low-stress transportation. This effectively solves the problem of low survival rate caused by insufficient physical preparation, inconsistent size, and transportation stress during the transportation of fish from land to the deep sea.

[0094] In one optional implementation, the set water temperature is 23°C to 25°C, and the second transport density is 6500 ind / m³ to 9000 ind / m³. The water truck and the water boat are equipped with a circulating water filtration and purification system, which includes a mechanical filtration unit, a biological filtration unit, a protein separation unit, and an ultraviolet disinfection unit. During the transfer process, the dissolved oxygen in the transferred water is ≥5mg / L, and the ammonia nitrogen is <0.3mg / L. The water truck and the water boat use a dual oxygen supply method of pure oxygen and air oxygen, and the water is changed once every 4 hours during the transfer.

[0095] It should be noted that a circulating water filtration and purification system refers to a combination of equipment that continuously purifies the transported water. It maintains the cleanliness of the transported water through the synergistic effects of physical filtration, biodegradation, foam separation, and ultraviolet disinfection. The mechanical filtration unit intercepts suspended particulate matter and solid waste in the water using filter screens or filter media, forming the first physical barrier in water purification. The biological filtration unit utilizes beneficial microorganisms attached to the filter media to convert ammonia nitrogen and nitrite in the water into nitrates, a crucial step in maintaining the chemical stability of the water. The protein separation unit separates dissolved organic matter and tiny suspended particles from the water using the principle of air flotation, reducing the accumulation of organic matter. The ultraviolet disinfection unit kills pathogenic microorganisms in the water through ultraviolet irradiation, preventing the spread of pathogens during transportation. A water temperature range of 23 to 25 degrees Celsius is suitable for medium-sized oval pomfret during transportation. Within this temperature range, the fish's metabolic rate is moderate, avoiding both excessive metabolism leading to increased oxygen consumption and excrement, and excessively low temperatures causing decreased fish vitality.

[0096] In this embodiment, this application provides a specific implementation plan for water quality maintenance and parameter control during the transfer of live water trucks and boats. By setting the water temperature to 23°C to 25°C and the second transport density to 6500 fish / cubic meter to 9000 fish / cubic meter, precise temperature control effectively reduces the metabolic rate of fish during transport, reducing oxygen consumption and excrement production. Reasonable transport density ensures transport efficiency while avoiding water quality deterioration and fish overcrowding stress caused by excessive density. By equipping the live water trucks and boats with a circulating water filtration and purification system including a mechanical filtration unit, a biological filtration unit, a protein separation unit, and an ultraviolet disinfection unit, multi-stage purification of the transport water is achieved. Mechanical filtration removes suspended particulate matter, biological filtration converts ammonia nitrogen to nitrate, protein separation removes dissolved organic matter, and ultraviolet disinfection kills pathogenic microorganisms. The synergistic effect of these four processes ensures stable water quality during transport. The water quality standards of dissolved oxygen not lower than 5 mg / L and ammonia nitrogen lower than 0.3 mg / L during transport provide the fish with sufficient dissolved oxygen and a low-ammonia nitrogen growth environment. By employing a dual oxygen supply method of pure oxygen and air oxygen, and changing the water once every 4 hours during the transport time, the dual oxygen supply ensures the reliability and redundancy of dissolved oxygen supply. Regular water changes also promptly remove metabolic products accumulated during transportation, effectively preventing water quality deterioration.

[0097] Overall, this implementation method provides a stable and clean aquatic environment for fish species during long-distance transportation through multiple safeguards such as precise temperature control, circulating water purification, dual oxygen supply, and regular water changes, effectively ensuring the survival rate during transportation.

[0098] Based on the first embodiment of this application, in the fourth embodiment of this application, the content that is the same as or similar to that in the first embodiment can be referred to the above description, and will not be repeated hereafter. Based on this, please refer to... Figure 4 Step S30 includes steps S31 to S38: Step S31: When the current is calm, release the medium-sized fish fry into the aquaculture cage at a second stocking density of 40 ind / m³ to 60 ind / m³. It should be noted that calm currents refer to periods when the periodic horizontal flow of seawater is at a low level, typically occurring during the transition between high and low tides. Releasing fish fry during calm periods reduces the impact of water flow on the fry, minimizing stress and energy expenditure during the initial placement in the cages. The second point refers to the stocking density when medium-sized fish fry are released into deep-sea aquaculture cages. A suitable stocking density range for this size of fish in deep-sea cages is 40 to 60 fry per cubic meter. This density ensures sufficient growth space and a good water quality environment while also maximizing the utilization of the cage culture space.

[0099] In step S31, after the live-water vessel arrives at the deep-sea aquaculture area, the executing entity determines the current tidal trend based on the tide table and selects a period with calm tidal currents for stocking. The executing entity transfers the fish fry from the live-water tank of the live-water vessel to the aquaculture cages, controlling the stocking density to be within the range of 40 fish / cubic meter to 60 fish / cubic meter.

[0100] In one example, the implementing entity determines, based on the tide table, that the current period is a period of calm tide during the transition period, and begins releasing fish fry. The total number of medium-sized fish fry transported by the live-water vessel is 750,000, which are released into multiple aquaculture cages with a total water volume of 15,000 cubic meters, with an average stocking density of 50 fish per cubic meter.

[0101] In another example, the implementing entity chose to release fish fry during the low tide period at a stocking density of 45 fish per cubic meter.

[0102] Step S32: The medium-sized fish fry are cultivated through the second feeding management; the second feeding management is to feed 2 to 3 times a day, during the slow tide or slack tide period, and the daily feeding amount is 5% to 8% of the fish body weight. The feed is a special extruded compound feed for oval pomfret. It should be noted that the period of slow or slack tide refers to the time when the tidal current speed changes from rapid to slow or the water is basically still. Oval pomfret have the habit of feeding against the current. During slow or slack tides, the current speed is moderate or the water is still, and the feed is not easily dispersed by the current. The fish can effectively feed during the settling and diffusion of the feed, resulting in the highest feeding efficiency. During rapid tides, the current speed is too fast, and the feed is easily dispersed and lost. The fish also experience a decrease in appetite because they need to expend a lot of energy to maintain their position. Oval pomfret-specific extruded compound feed is a granular feed specially formulated according to the nutritional needs of oval pomfret. The extrusion process gives the feed good stability in water.

[0103] In step S32, the executing entity determines the daily feeding time based on tidal conditions, selecting periods of slow or slack tide for feeding. The executing entity adds extruded compound feed specifically for oval pomfret into the net cages, with the feeding amount being 5% to 8% of the total fish weight, fed 2 to 3 times daily. During the feeding process, the executing entity observes the feeding activity of the fish, using the completion of feeding within a specified time as the criterion for determining the appropriate feeding amount.

[0104] In one example, the operator determines the slow tide periods each day as 8:00-9:00 AM and 3:00-4:00 PM based on the local tide table. Feeding is conducted once during each of these periods, with a daily feed amount of 6% of the fish's body weight, evenly distributed each time. The feed used is a specially formulated extruded feed for oval pomfret, with the feed particle size matching the mouth width of the fish.

[0105] In another example, the implementer feeds the fish three times a day, once during the morning low tide, once during the afternoon low tide, and once during the evening slack tide, with a daily feed amount of 7% of the fish's body weight.

[0106] Step S33: Reduce or stop feeding based on weather conditions, water quality, and fish disease status. In step S33, the implementing entity assesses the weather conditions, water quality, and fish health status before daily feeding. If there is continuous rain, high temperature, strong wind, or other severe weather, or if water quality monitoring results show low dissolved oxygen or high ammonia nitrogen, or if the fish show suspected disease symptoms such as decreased feeding or abnormal swimming, the implementing entity will proactively reduce the amount of feed or stop feeding completely, and resume normal feeding after the conditions improve.

[0107] In one example, the implementing entity detected a drop in air pressure and an increase in wind speed before the typhoon arrived, and proactively halved the daily feeding amount. Feeding was stopped during the typhoon, and the feeding amount was gradually restored after the typhoon when the water quality stabilized and the fish activity returned to normal.

[0108] In another example, when the implementer detected water temperatures exceeding 33 degrees Celsius during the summer heat, it proactively reduced the amount of feed to 50%-60% of the normal amount.

[0109] Step S38: When the medium-sized fish grows to a weight of 50g / ind to 125g / ind, the large-sized fish is obtained.

[0110] In step S38, the implementing entity periodically samples and measures the weight of the fish fry during the deep-sea cultivation process to monitor their growth progress. When the sampling measurement results show that the average weight of the fish population reaches the range of 50 grams / fish to 125 grams / fish, the implementing entity confirms that the fish fry have grown to the large-sized stage and can proceed to the hatching and thinning stage.

[0111] In one example, the implementing entity sampled and measured the weight of the fish fry every 10 days during the deep-sea rearing period. At the time of release, the average weight of the medium-sized fish fry was 9 grams per fish. After 60 days of rearing, the sampling measurement showed that the average weight had reached 80 grams per fish, and the implementing entity confirmed that the fish fry had grown to a large size.

[0112] In another example, after 75 days of cultivation, the fish reached an average weight of 100 grams per fish, confirming that they met the standard for large-sized fish.

[0113] In this embodiment, this application provides a specific implementation plan for the stocking and feeding management of medium-sized fish fry during the deep-sea cultivation stage. Firstly, medium-sized fish fry are stocked at a second stocking density of 40 to 60 fish per cubic meter during periods of calm tides. Stocking during calm tides reduces the impact of water flow and stress on the fry in the initial stage of entering the cages. The reasonable stocking density provides ample growth space and a good water quality environment for the fry, balancing aquaculture efficiency and healthy fish growth. Then, a second feeding management plan is implemented, feeding 2 to 3 times daily, during periods of slow or equidistant tides, with a daily feed amount of 5% to 8% of the fish's body weight. This plan incorporates the behavioral principles of oval pomfret feeding upstream. Feeding during slow or equidistant tides ensures that the feed is fully dispersed within the cages and effectively consumed by the fry, avoiding feed loss and low feeding efficiency caused by feeding during periods of rapid tides. Specialized extruded compound feed provides the fry with a comprehensive and balanced nutritional supply. Furthermore, by flexibly reducing or stopping feeding based on weather conditions, water quality, and fish disease status, we avoided feed waste and increased water pollution caused by feeding under unfavorable conditions, as well as the problem of increasing the digestive burden on fish during illness. Finally, by identifying large-sized fish when medium-sized fry grow to 50-125 grams per fish, we established clear stage transition standards.

[0114] Overall, this embodiment provides comprehensive protection for the rapid and healthy growth of fish fry during the deep-sea cultivation stage through scientific stocking density, feeding management based on tidal rhythms, and flexible measures to deal with abnormal situations.

[0115] Based on the first embodiment of this application, in the fifth embodiment of this application, the same or similar content as the first embodiment can be referred to the above description, and will not be repeated hereafter.

[0116] Based on this, the deep-sea aquaculture area is ≥10km from the shore, with an average water depth ≥13m, a current velocity of 0.2m / s to 1.0m / s, a seawater salinity of 18 to 32, a water temperature of 23℃ to 32℃, and dissolved oxygen ≥5 mg / L; The aquaculture cage is set as an HDPE gravity cage, including an HDPE frame system, a PE netting system and an anchoring system. The mesh size of the HDPE gravity cage is 2cm to 4cm. The arrangement direction of the multiple aquaculture cages is consistent with the tidal flow direction, and the distance between adjacent aquaculture cages is ≥100 m.

[0117] It should be noted that high-density polyethylene (HDPE) gravity net cages refer to deep-sea aquaculture net cages that utilize a buoyancy frame made of HDPE material to provide buoyancy, relying on their own weight to keep the netting in an extended state in the water. HDPE material has advantages such as UV resistance, seawater corrosion resistance, high strength, and good toughness, making it suitable for long-term use in harsh deep-sea environments. The polyethylene netting refers to netting woven from polyethylene material, which is characterized by high strength, low water absorption, and corrosion resistance. The mesh size of the netting is 2 to 4 centimeters, a range that matches the body size of medium to large-sized fish, preventing escape while ensuring sufficient water exchange between the inside and outside of the net cage.

[0118] In this embodiment, this application provides a specific implementation plan for the environmental conditions and cage facility configuration of the deep-sea aquaculture area. By setting environmental conditions for the deep-sea aquaculture area with a distance from the shore of not less than 10 kilometers, an average water depth of not less than 13 meters, a current velocity of 0.2 m / s to 1.0 m / s, a salinity of 18 to 32, a water temperature of 23 to 32 degrees Celsius, and dissolved oxygen of not less than 5 mg / L, the cultivation area is ensured to have excellent environmental characteristics such as clean water, sufficient dissolved oxygen, and suitable current velocity, far away from nearshore eutrophic areas, fundamentally reducing the environmental risk of disease occurrence. By adopting high-density polyethylene gravity cages and configuring a high-density polyethylene frame system, a polyethylene net system, and an anchoring system with a mesh size of 2 cm to 4 cm, a stable aquaculture facility suitable for the deep-sea environment is provided. The matching of the net mesh size with the size of the fish species prevents escape and ensures water exchange. By arranging multiple aquaculture cages in the same direction as the tidal current and maintaining a minimum distance of 100 meters between adjacent cages, the impact of the tidal current on the cage structure was reduced. This also ensured uninterrupted water exchange between cages and reduced the risk of cross-infection of diseases. Overall, this embodiment provides suitable environmental conditions and stable facilities for the deep-sea cultivation stage, ensuring the healthy growth of fish fry in the deep-sea environment.

[0119] Based on the first embodiment of this application, in the sixth embodiment of this application, the same or similar content as the first embodiment described above can be referred to the above description, and will not be repeated hereafter. Based on this, please refer to... Figure 5 In step S30, the step of further cultivating the medium-sized fish fry to large-sized fish fry also includes daily management steps S34~S37: Step S34: Inspect and replace or clean the mesh every 15 to 30 days. The cleaning method is to spray with a high-pressure water gun or expose to the sun and beat it to remove the mesh. It should be noted that netting replacement or cleaning refers to the regular maintenance of the netting in aquaculture cages. In deep-sea environments, the netting is immersed in seawater for extended periods, and its surface gradually accumulates biofouling materials such as algae, shellfish larvae, and hydra. These biofouling materials clog the mesh, reducing the exchange efficiency of water inside and outside the cage, and affecting the supply of dissolved oxygen and the removal of metabolic waste. High-pressure water jet cleaning refers to a physical cleaning method that uses high-pressure water jets to impact the surface of the netting to remove biofouling materials. This method is suitable for in-situ cleaning when there are few biofouling materials. Sun-drying and beating removal refers to a method where the netting is lifted out of the water and exposed to sunlight to dry and remove biofouling materials before beating them off. This method is suitable for thorough cleaning when there are many biofouling materials.

[0120] In step S34, the implementing entity regularly checks the attachment status of the nets during the deep-sea cultivation process. The implementing entity conducts a comprehensive inspection of the nets every 15 to 30 days to assess the quantity and type of attachments. When there are few attachments, the implementing entity uses a high-pressure water gun to spray and wash the nets in situ, controlling the pressure within an appropriate range to avoid damaging the nets. When there are many attachments, the implementing entity pre-installs spare nets, transfers the fish to the spare nets, then lifts the old nets out of the water, exposes them to the sun, and then beats them to remove the attachments.

[0121] In one example, the implementing entity inspects and cleans the mesh every 25 days. When there is little residue, a high-pressure water gun is used for in-situ cleaning at a pressure of 0.4 MPa, rinsing the mesh surface row by row from top to bottom until the residue is completely removed. When there is a lot of residue, the implementing entity replaces the mesh with a spare and then exposes the old mesh to direct sunlight for 24 hours and beats it to remove the residue.

[0122] In another example, the implementing entity inspects the netting every 15 days and replaces and cleans the netting when it finds a large amount of attached material.

[0123] Step S35: During the disease outbreak season, add an immune enhancer to the feed and feed it to the animals. It should be noted that the disease epidemic season refers to a specific period determined based on past aquaculture experience and the epidemiological patterns of the sea area, during which the incidence of specific diseases is significantly higher than at other times. Immunostimulants are bioactive substances that can enhance the non-specific immune function of farmed fish, including vitamin C, vitamin E, glucan, and peptidoglycan. They improve the fish's disease resistance by activating immune cells, promoting antibody production, or enhancing complement activity.

[0124] In step S35, the implementing entity predicts the disease epidemic season based on the disease occurrence patterns and environmental conditions of previous years. After the disease epidemic season begins, the implementing entity regularly adds immune enhancers to the feed and feeds it to the fish. After feeding for a certain number of consecutive days, it pauses for a period of time before starting the next round of feeding.

[0125] In one example, the implementing entity determines from past experience that July to September is the peak season for streptococcal disease. Starting in late June, vitamin C and multivitamin preparations are added to the feed every 15 days. After feeding for 7 consecutive days, the feeding is paused for 7 days before the next round of feeding is carried out until the end of the peak season.

[0126] Step S36: When parasitic diseases occur, freshwater immersion and / or drug immersion shall be used for treatment; wherein, the parasitic diseases include Cryptocaryon irritans disease, Amylodinosis, and Benedenia disease; It should be noted that freshwater immersion refers to a treatment method in which diseased fish are transferred from a marine environment to a freshwater environment for a short period of immersion. Freshwater immersion utilizes the osmotic pressure difference between freshwater and seawater, causing parasites living on the fish's body surface or gills to detach due to the dramatic change in osmotic pressure, thus achieving the purpose of eliminating parasites. Medicated immersion refers to a treatment method in which specific drugs are added to the freshwater for immersion; the drug components can further enhance the killing effect on parasites.

[0127] In step S36, the implementing entity observes the fish population during routine patrols for typical symptoms of parasitic diseases, including fish rubbing against the netting, increased mucus on the body surface, abnormal swimming, and gill lesions. When a parasitic disease is confirmed or highly suspected, the implementing entity selects the appropriate treatment method based on the type of pathogen. For example, Cryptocaryon irritans infection can be treated with a freshwater bath followed by soaking in a mixed solution of copper sulfate and magnesium sulfate; Amylodinium infection can be treated with various methods such as freshwater soaking, potassium permanganate solution soaking, or copper sulfate solution soaking; Benedenia infection can be treated with freshwater soaking.

[0128] In one example, the implementers observed that the fish in the net cages exhibited symptoms such as frequent rubbing against the netting and the appearance of white mucus patches on their bodies, which were confirmed by microscopic examination to be caused by Cryptocaryon irritans infection. After removing the fish from the net cages, the implementers placed them in a freshwater container for an 8-minute freshwater bath. Following the bath, the fish were transferred to a mixed solution of copper sulfate and magnesium sulfate at a mass-to-volume ratio of 1:50000 and immersed for 25 minutes. After treatment, the fish were returned to the net cages.

[0129] In another example, after the subject was diagnosed with amyloidosis, it was treated by soaking in fresh water for 8 minutes.

[0130] Step S37: When bacterial diseases occur, medicated feed is mixed with feed for treatment; wherein, the bacterial diseases include streptococcal disease and bacterial enteritis.

[0131] It should be noted that medicated feed refers to feed containing therapeutic drugs mixed into the feed in a predetermined ratio. By administering medicated feed, the fish ingest the medication, thereby inhibiting and killing bacterial pathogens within their bodies. Mixing feed refers to the process of thoroughly mixing the medication with the feed, ensuring that the medication is evenly distributed throughout the feed.

[0132] In step S37, the operator observes the fish population during routine patrols for typical symptoms of bacterial diseases, including bulging eyes, corneal opacity, surface bleeding, unbalanced swimming, cessation of feeding, and redness and swelling of the anus. When a bacterial disease is diagnosed, the operator selects the appropriate treatment drug based on the type of pathogen, calculates the dosage according to the total weight of the fish population, mixes the drug with the feed to make medicated feed, and then feeds the fish.

[0133] In one example, the operator discovered that the fish in the net cage exhibited symptoms such as bulging eyes, corneal opacity, and surface bleeding. Pathogen isolation and identification confirmed streptococcal infection. After estimating the total weight of the fish, the operator selected doxycycline hydrochloride and mixed the drug with the feed according to the dosage recommended in the drug's instructions to prepare medicated feed. This feed was administered for 5 consecutive days, during which the activity of the fish was closely observed.

[0134] In another example, after the subject was diagnosed with bacterial enteritis, enrofloxacin was mixed with feed and fed for 5 consecutive days.

[0135] In this embodiment, this application provides a specific implementation plan for daily management during deep-sea aquaculture. First, the netting is inspected and replaced or cleaned every 15 to 30 days using high-pressure water jets or sun-drying and beating to remove biological fouling. This regular cleaning process maintains unobstructed water exchange inside and outside the net cages, ensuring the stability of the aquaculture environment and preventing problems such as decreased dissolved oxygen and accumulation of metabolic waste due to clogged mesh. Then, during disease outbreaks, immune enhancers are added to the feed. The non-specific immune activation effect of these enhancers improves the overall disease resistance of the fish population, achieving proactive disease prevention. Furthermore, when parasitic diseases such as Cryptocaryon irritans, Amylodinium oocystis, and Benedeniasis occur, freshwater immersion and / or medicated immersion are used. The osmotic pressure difference between freshwater and seawater helps the parasites detach from the fish, and the synergistic effect of medication enhances the deworming effect, achieving effective treatment of parasitic diseases. Finally, when bacterial diseases such as streptococcal disease and bacterial enteritis occur, medicated feed is used for treatment, allowing the therapeutic drugs to enter the fish's body through ingestion, effectively inhibiting and killing bacterial pathogens within the body. Overall, this embodiment, through multi-level management measures including regular netting maintenance, immune enhancer prevention, freshwater immersion for parasitic diseases, and medicated feed treatment for bacterial diseases, constructs a complete disease control system covering prevention and treatment, effectively ensuring the healthy growth of fish populations during the deep-sea cultivation stage.

[0136] Based on the first embodiment of this application, in the seventh embodiment of this application, the same or similar content as the first embodiment described above can be referred to the above description, and will not be repeated hereafter. Based on this, please refer to... Figure 6 Following step S30, the method for cultivating large-sized oval pomfret fry in deep-sea and land-sea relay also includes steps S41-S44: Step S41: Stop feeding the large-sized fish for 1 day, gather them together with a net, and then divide the fish. It should be noted that "stopping feeding" refers to ceasing feeding before the fish are separated and thinned out. Stopping feeding for one day allows the fish to fully empty any remaining food from their digestive tracts, reducing water pollution caused by the accumulation of excrement during separation and transportation. "Netting and gathering" refers to using fishing nets to gradually drive the fish in the cages and gather them in a corner or specific area of ​​the cage, facilitating subsequent separation and harvesting.

[0137] In step S41, after determining that the large-sized fish fry have reached the hatching standard, the executing entity stops feeding them one day before the scheduled hatching date. During the period of withholding feeding, the executing entity maintains normal water exchange and management operations in the net cage, but no further feeding is provided. One day after withholding feeding, the executing entity slowly advances the net cage from one end to the other using a fishing net, gradually driving the fish and gathering them in the designated area of ​​the net cage. After the fish have gathered, the executing entity performs a fish separation operation, catching the fish in batches.

[0138] In one example, the operator stopped feeding the fish at 8:00 AM the day before the scheduled hatching date, and the feeding period lasted for a full 24 hours until the fish were distributed the following day. During the feeding stoppage, the operator maintained normal water quality management in the net cages. The following morning, the operator used fishing nets to gather the fish into a corner of the net cage and then scooped them out in batches for distribution.

[0139] Step S42: The large-sized fish fry are transported to the rearing cages using a live-water boat, and adult fish are raised in the rearing cages; the transport density of the live-water boat is 120 ind / m³ to 140 ind / m³. It should be noted that "adultery cages" refer to cage facilities used for raising adult fish. They are typically large in size and located in sea areas suitable for adult fish growth. A transport density of 120 to 140 fish per cubic meter is the suitable density range for transporting large-sized fish on live-water vessels. At this density, the fish have ample space to move around, and the water quality of the transport water can remain stable.

[0140] In step S42, the executing entity loads the larger fish fry after separation into the live water tank of the live water vessel, and activates the vessel's circulating water system and oxygenation system to transport the fish fry from the deep-sea rearing cages to the adult rearing cages. The transport density is controlled within the range of 120 to 140 fish fry per cubic meter. Upon arrival at the adult rearing cages, the executing entity releases the fish fry into the cages, beginning the adult fish farming stage.

[0141] In one example, the operator loads the larger fish fry, after separation, into a live-water vessel. The live-water vessel has a 100-cubic-meter live-water compartment, carrying 13,000 large-sized fish fry at a transport density of 130 fish per cubic meter. The live-water vessel transports the fry from the rearing cages to the adult rearing cages, a process that takes 2 hours. Upon arrival, the operator releases the fry into the adult rearing cages for further cultivation.

[0142] In another example, the operator loads large-sized fish fry onto ships and transports them to rearing cages at a transport density of 125 fish per cubic meter.

[0143] Step S43: Before the large-sized fish fry are hatched, a second quality test is performed to obtain the second quality test result; It should be noted that the second quality inspection refers to a comprehensive inspection and evaluation of the fish population's quality before the large-sized fish fry are separated and separated. Its purpose is to ensure that the quality of the fry meets the requirements for adult fish farming.

[0144] In step S43, the executing entity conducts quality testing on the fish population before the large-sized fish fry are released. The executing entity randomly selects a certain number of samples from the fish population and examines the samples for body shape, body color, fin integrity, scale integrity, physical condition, and reaction speed. Samples are also taken for pathogen testing, with a focus on detecting whether they carry neuronecrosis virus, Cryptocaryon irritans, and Benedenella.

[0145] In one example, the implementing entity randomly selected 100 fish from a large-sized fry population for quality testing before hatching. The test results showed that all samples had normal body shape and color, intact fins and scales, robust constitution, and sensitive reactions. Pathogen detection did not detect rhabdovirus, neuronecrosis virus, Cryptocaryon irritans, or Benedenella. The second quality test result was qualified.

[0146] Step S44: If the second quality inspection result is qualified, then the large-sized fish fry are harvested.

[0147] In step S44, the executing entity makes a judgment based on the second quality inspection results. If all inspection indicators are within the qualified standard range, the quality of the batch of large-sized fish is confirmed to be qualified, and they can be harvested and released into the rearing cages for adult fish farming.

[0148] In one example, after the implementing entity confirms that the second quality inspection result is qualified, all the large-sized fish fry are transferred to the rearing cages to begin the adult fish farming stage.

[0149] In another example, the detection process revealed that some fish species had fin damage. These individuals were removed, and the remaining qualified fish species were harvested and transferred.

[0150] In this embodiment, this application provides a specific implementation plan for the separation and distribution of large-sized fish fry. First, by withholding feed from the large-sized fish fry for one day, residual feed in their digestive tracts is fully emptied, effectively reducing the risk of water pollution caused by the accumulation of excrement during separation and transportation, and ensuring the cleanliness of the transport water. Then, the fish are separated after being gathered by a seine net. Using a live-water vessel, the large-sized fish fry are transported to rearing cages at a density of 120 to 140 fish per cubic meter for adult rearing. This reasonable transport density ensures that the fish fry have ample space to move and a good water quality environment during transportation. The continuous oxygenation and circulating water purification provided by the live-water vessel ensure the physiological health of the fish fry during transportation. Furthermore, due to the consistency between the deep-sea rearing environment and the rearing cage environment, the fish fry do not need to undergo secondary environmental adaptation after transfer and can directly enter a rapid growth period. Furthermore, by conducting a second quality inspection covering multiple dimensions such as body shape, body color, fin integrity, scale integrity, physical condition, reaction speed, and pathogen status before large-sized fish fry are released, strict quality control standards for fry release have been established to ensure that the fry do not carry specific pathogens such as rhabdovirus, neuronecrosis virus, Cryptocaryon irritans, and Benedenella. Finally, large-sized fish fry are harvested only after passing the inspection, achieving full-chain quality control from fry to medium-sized fish fry to large-sized fish fry. Overall, this embodiment, through multiple safeguards such as withholding feed and emptying the tanks, temperature-controlled live-water transport, and quality inspection, has achieved efficient and low-loss transfer of large-sized fish fry to rearing cages, laying a solid foundation for rapid growth and high-efficiency production in the subsequent adult fish farming stage.

[0151] For example, to help understand the implementation process of the method for cultivating large-sized oval pomfret fry in deep-sea and land-sea relays, which is obtained by combining this embodiment with any of the above embodiments, please refer to... Figure 7 , Figure 7 A simplified flowchart is provided for a method of cultivating large-sized oval pomfret fry in deep-sea, land-sea relay farming. Specifically: First, oval pomfret fry were released into the land-based breeding facility. The oval pomfret fry had a size of 0.4g / fish to 0.6g / fish. Secondly, in land-based cultivation facilities (i.e., land-based ponds), oval pomfret fry are raised to medium-sized fish of 8g / fish to 10g / fish; Then, the medium-sized fish species were graded, screened, and trained in water in sequence. Next, the medium-sized fish fry that have been graded, screened, and acclimated in water are transported to the deep-sea aquaculture area using temperature-controlled circulating water via live water trucks and live water boats. Afterwards, medium-sized fish fry continued to be raised in aquaculture cages in deep-sea aquaculture areas (i.e., a two-stage culturing process). Finally, large-sized fish ranging from 50g to 125g were bred.

[0152] It should be noted that the above examples are only for understanding this application and do not constitute a limitation on the method for cultivating large-sized oval pomfret in deep-sea land-sea relay. Any simple modifications based on this technical concept are within the scope of protection of this application.

[0153] The above description is merely an exemplary embodiment of this application and does not limit the patent scope of this application. Any equivalent structural transformations made based on the technical concept of this application and the contents of the specification and drawings of this application, or direct / indirect applications in other related technical fields, are included within the patent protection scope of this application.

Claims

1. A method for cultivating large-sized oval pomfret fry in deep-sea and land-sea relay systems, characterized in that, include: Oval pomfret fry are released into a land-based aquaculture facility and then raised to medium-sized fingerlings. After the medium-sized fish fry are graded, screened, and trained in water, they are transferred to the deep-sea aquaculture area. The medium-sized fish fry are released into the aquaculture cages in the deep-sea aquaculture area and then further cultivated into large-sized fish fry.

2. The method for cultivating large-sized oval pomfret fry in deep-sea and land-sea relay as described in claim 1, characterized in that, The step of releasing oval pomfret fry into a land-based cultivation facility and cultivating the oval pomfret fry to medium-sized fingerlings includes: Obtain qualified oval pomfret fry; the oval pomfret fry have a size of greater than or equal to 0.4 g / ind and less than or equal to 0.6 g / ind; After cleaning, disinfecting, and filling the land-based cultivation facility with water, the oval pomfret fry are released at the first stocking density; The oval pomfret fry were cultivated in accordance with the first feeding management plan and the first water quality management plan; When the oval pomfret fry grow to a weight greater than or equal to 8 g / ind and less than or equal to 10 g / ind, the medium-sized fish species are obtained.

3. The method for cultivating large-sized oval pomfret fry in deep-sea and land-sea relay farming as described in claim 2, characterized in that, The oval pomfret fry are at least free of specific pathogens, which include at least rhabdovirus, neuronecrosis virus, Cryptocaryon irritans, and Amylodinium oogonium; the land-based cultivation facility is a pond; The step of cleaning, disinfecting, and filling the land-based cultivation facility with water, and then releasing the oval pomfret fry at a first stocking density, includes: After drying the pond and exposing it to the sun for 10 to 15 days, disinfect the entire pond by spraying it with chlorinated lime. After cleaning the pond for 7 days, fill it with water. Before stocking, take a sample of the water body to be stocked, and put the same type of fish fry into the water sample and observe for 24 hours to test the water quality. After confirming through water testing that the same type of fish fry are active and feeding normally, the oval pomfret fry are released at a first stocking density of 12,000 to 20,000 fry / mu, with a stocking water temperature ≥23℃ and a temperature difference of ≤2℃ between the stocking water temperature and the transport water temperature.

4. The method for cultivating large-sized oval pomfret fry in deep-sea and land-sea relay as described in claim 2, characterized in that, The first feeding management involves feeding the fish 3 to 4 times a day, with a daily feed amount of 8% to 12% of the fish's body weight. The first water quality management plan includes: not changing the water in the early stage of stocking, only adding a small amount of water every day; after 7 days of stocking, changing 10% to 20% of the water every day; during the breeding process, adjusting the water quality by turning on the aerator, changing the water, or sprinkling water quality regulators according to the water color, water temperature, and dissolved oxygen of the pond. The first feeding management plan includes adding at least one of allicin, immunopolysaccharide, or traditional Chinese medicine preparation to the feed; And / or, the step of obtaining the medium-sized fish fry when the oval pomfret fry grow to a weight greater than or equal to 8 g / ind and less than or equal to 10 g / ind includes: When the oval pomfret fry grow to a weight greater than or equal to 8 g / ind and less than or equal to 10 g / ind, a first quality test is performed to obtain the first quality test result; the first quality test includes at least the following: body shape, body color, fin integrity, scale integrity, physical condition, reaction speed, deformity rate, injury rate, and pathogen status; If the first quality test result is qualified, then the medium-sized fish species is obtained.

5. The method for cultivating large-sized oval pomfret fry in deep-sea and land-sea relay as described in claim 1, characterized in that, The step of sequentially grading, screening, and acclimating the medium-sized fish fry before transferring them to a deep-sea aquaculture area includes: The medium-sized fish species are divided into 2 to 3 size grades according to their body weight, so that the size of fish species within the same grade remains consistent. After grading, the medium-sized fish fry were placed in a directional water flow for 30 to 60 minutes to allow them to empty their feces, reduce the amount of mucus on their bodies, and adapt to the dense environment. The medium-sized fish fry, after being exercised in the water, are transferred to the deep-sea aquaculture area at a second transport density using a live water truck and a live water boat in sequence; wherein, the live water truck and the live water boat continuously supply oxygen and maintain a set water temperature during the transfer process.

6. The method for cultivating large-sized oval pomfret fry in deep-sea and land-sea relay farming as described in claim 5, characterized in that, The set water temperature is 23°C to 25°C, and the second transport density is 6500 ind / m³ to 9000 ind / m³; The water truck and the water boat are equipped with a circulating water filtration and purification system, which includes a mechanical filtration unit, a biological filtration unit, a protein separation unit, and an ultraviolet disinfection unit. During the transfer process, the dissolved oxygen in the transferred water is ≥5mg / L, and the ammonia nitrogen is <0.3mg / L. The water truck and the water boat use a dual oxygen supply method of pure oxygen and air oxygen, and the water is changed once every 4 hours during the transfer.

7. The method for cultivating large-sized oval pomfret fry in deep-sea and land-sea relay as described in claim 1, characterized in that, The step of releasing the medium-sized fish fry into the aquaculture cages in the deep-sea aquaculture area and further cultivating the medium-sized fish fry to large-sized fish fry includes: When the current is calm, the medium-sized fish fry are released into the aquaculture cages at a second stocking density of 40 ind / m³ to 60 ind / m³. The medium-sized fish species are cultivated through a second feeding management method. The second feeding management method involves feeding the fish 2 to 3 times a day, during periods of slow or calm tides, with a daily feeding amount of 5% to 8% of the fish's body weight. The feed used is a special extruded compound feed for oval pomfret. Depending on the weather, water quality, and the fish's condition, reduce or stop feeding. When the medium-sized fish grows to a weight of 50g / ind to 125g / ind, the large-sized fish is obtained.

8. The method for cultivating large-sized oval pomfret fry in deep-sea and land-sea relay as described in claim 1, characterized in that, The deep-sea aquaculture area is located ≥10km from the shore, with an average water depth ≥13m, a current velocity of 0.2m / s to 1.0m / s, a seawater salinity of 18 to 32, a water temperature of 23℃ to 32℃, and dissolved oxygen ≥5 mg / L. The aquaculture cage is set as an HDPE gravity cage, including an HDPE frame system, a PE netting system and an anchoring system. The mesh size of the HDPE gravity cage is 2cm to 4cm. The arrangement direction of the multiple aquaculture cages is consistent with the tidal flow direction, and the distance between adjacent aquaculture cages is ≥100m.

9. The method for cultivating large-sized oval pomfret fry in deep-sea and land-sea relay farming as described in any one of claims 1 to 8, characterized in that, The step of further cultivating the medium-sized fish fry to large-sized fish fry also includes daily management steps: Inspect and replace or clean the netting of the aquaculture cages every 15 to 30 days. The cleaning method is to spray with a high-pressure water gun or expose to the sun and beat it to remove the netting. During disease outbreak seasons, add immune enhancers to the feed before feeding; When parasitic diseases occur, freshwater immersion and / or drug immersion shall be used for treatment; wherein, the parasitic diseases include Cryptocaryon irritans infection, Amylodinosis, and Benedenia infection; When bacterial diseases occur, medicated feed is used for treatment; the bacterial diseases include streptococcal disease and bacterial enteritis.

10. The method for cultivating large-sized oval pomfret fry in deep-sea and land-sea relay farming as described in any one of claims 1 to 8, characterized in that, The method for cultivating large-sized oval pomfret fry in deep-sea and land-sea relay also includes: For the large-sized fish, stop feeding for 1 day, gather them together with nets, and then divide the fish. The large-sized fish fry are transported to rearing cages using live-water vessels, where they are then cultured as adult fish; the transport density of the live-water vessels is 120 ind / m³ to 140 ind / m³. A second quality test was conducted before the large-sized fish fry were hatched, and the results of the second quality test were obtained. If the second quality inspection result is qualified, the large-sized fish fry will be harvested.

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