Facility fruit and vegetable soil-borne disease antagonistic microorganism compound disease-preventing microbial agent

Abstract

The application discloses a facility fruit and vegetable soil-borne disease antagonistic microbial compound disease-preventing agent, which comprises a functional microbial composition, a nutrient carrier and an additive; wherein the component ratio is as follows: the functional microbial composition is 5-15 parts, the nutrient carrier is 60-85 parts, and the additive is 0.5-5 parts; the functional microbial composition is at least two kinds of Bacillus microbial, Trichoderma microbial and Pseudomonas microbial mixed according to a mass ratio; the nutrient carrier is obtained by mixing organic matter matrix and inorganic mineral matrix according to a mass ratio of 3-7:7-3; and the additive comprises a dispersing agent and a water-retaining agent, and the mass ratio of the dispersing agent to the water-retaining agent is 1-3:1-2. Compared with the prior art, the application has the advantages that a facility fruit and vegetable soil-borne disease antagonistic microbial compound disease-preventing agent with multiple strains synergistically antagonizing and stable field prevention effect is provided.

Description

Technical Field

[0001] This invention relates to the field of biological control technology, specifically to a compound antibacterial agent containing antagonistic microorganisms against soil-borne diseases in greenhouse fruits and vegetables. Background Technology

[0002] Due to continuous cropping obstacles, high humidity, and enclosed soil, greenhouse fruit and vegetable cultivation suffers from severe soil-borne diseases, mainly including Fusarium wilt, root rot, Verticillium wilt, and damping-off. The main pathogens are Fusarium, Rhizoctonia, Pythium, and Phytophthora. While chemical pesticides are effective in controlling soil-borne diseases, long-term and excessive use leads to increased pathogen resistance, excessive pesticide residues, soil microecological imbalance, and environmental pollution, seriously threatening the sustainable production of greenhouse fruits and vegetables.

[0003] Microbial control is a green control method that uses beneficial microorganisms and their metabolites to inhibit or kill pathogens. It has advantages such as being environmentally friendly, not easily developing drug resistance, and promoting plant growth.

[0004] Existing microbial agents are difficult to control multiple soil-borne pathogens simultaneously, have poor field colonization ability, and are easily inactivated by soil environmental stress after application, affecting the uniform distribution and persistence of the agents in the soil. Summary of the Invention

[0005] The technical problem to be solved by this invention is to overcome the above-mentioned technical defects and provide a compound microbial agent for preventing soil-borne diseases of greenhouse fruits and vegetables with multi-strain synergistic antagonism and stable field control efficacy.

[0006] To solve the above-mentioned technical problems, the technical solution provided by the present invention is: a compound antibacterial agent for soil-borne diseases of greenhouse fruits and vegetables, comprising a functional microbial composition, a nutrient carrier, and adjuvants; The proportions of each component are as follows: 5-15 parts of functional microbial composition, 60-85 parts of nutrient carrier, and 0.5-5 parts of adjuvant. The functional microbial composition is obtained by mixing at least two of the following microorganisms in a certain mass ratio: Bacillus, Trichoderma, and Pseudomonas. The nutrient carrier is obtained by mixing an organic matrix and an inorganic mineral matrix in a mass ratio of 3-7:7-3. The additives include dispersants and water-retaining agents, with a mass ratio of dispersant:water-retaining agent = 1-3:1-2.

[0007] Preferably, the Bacillus microorganism is at least one of Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus natto, and Bacillus coagulans. The Trichoderma species mentioned are at least one of Trichoderma harzianum, Trichoderma viride, Trichoderma longibranchii, and Trichoderma echinosporum. The Pseudomonas species mentioned are at least one of Pseudomonas fluorescens, Pseudomonas aeruginosa, and Pseudomonas putida.

[0008] Preferably, the total number of viable bacteria in the functional microbial composition is greater than 2.0 × 10⁻⁶. 9 CFU / g; in: The effective viable count of Bacillus spp. is 1.0 × 10⁻⁶. 9 -5.0×10 9 CFU / g; The effective spore count of Trichoderma species is 1.0 × 10⁻⁶. 8 -1.0×10 9 CFU / g; The effective viable count of Pseudomonas spp. was 5.0 × 10⁻⁶. 8 -2.0×10 9 CFU / g.

[0009] Preferably, the organic matrix is ​​at least one of well-rotted farmyard manure, humic acid, amino acid fermentation broth, molasses, yeast extract powder, and soybean meal powder; The inorganic mineral matrix is ​​at least one of diatomaceous earth, zeolite powder, bentonite, vermiculite, kaolin, and attapulgite. The organic matrix has a carbon-to-nitrogen ratio of 15-30:1 and a water content of less than 20%.

[0010] Preferably, the dispersant is at least one selected from sodium lignosulfonate, sodium dodecylbenzenesulfonate, sodium dodecyl sulfate, polyethylene glycol, polyvinylpyrrolidone, and sodium carboxymethyl cellulose. The water-retaining agent is at least one of polyacrylamide, sodium polyacrylate, sodium alginate, sodium carboxymethyl cellulose, xanthan gum, and guar gum.

[0011] Preferably, the adjuvant further includes a pH adjuster and a biocontrol synergist; The pH adjuster is at least one of potassium dihydrogen phosphate, dipotassium hydrogen phosphate, calcium carbonate, calcium oxide, citric acid, and humic acid, and is added to maintain the pH value of the bacterial agent at 5.5-7.5. The biocontrol synergistic substance is at least one of chitin, chitosan, salicylic acid, methyl jasmonic acid, oligosaccharide and acetylsalicylic acid.

[0012] Preferably, the microbial agent further includes trace element components, the total mass of which accounts for 0.5%-2% of the total mass of the microbial agent.

[0013] Preferably, the trace element component is obtained by mixing at least two of zinc sulfate, ferrous sulfate, manganese sulfate, borax, sodium molybdate and copper sulfate in a mass ratio of 1-2:1-2:0.5-1.

[0014] The advantages of this invention compared to the prior art are: By combining Bacillus, Trichoderma, and Pseudomonas, synergistic antagonism among multiple strains is achieved, the antagonistic spectrum is broadened, and the nutrient carrier provides microorganisms with continuous nutrition and a suitable habitat, thus extending shelf life and field colonization capacity. This invention overcomes the limitations of single-strain control, the easy inactivation of inoculants, and the single function of inoculants, and achieves efficient and green control of soil-borne diseases in greenhouse fruits and vegetables. Detailed Implementation

[0015] The present invention will now be described in further detail.

[0016] The compound microbial agent for preventing soil-borne diseases in greenhouse fruits and vegetables includes a functional microbial composition, a nutrient carrier, and adjuvants, wherein the proportions of each component are: 5-15 parts of functional microbial composition, 60-85 parts of nutrient carrier, and 0.5-5 parts of adjuvants.

[0017] The functional microbial composition is obtained by mixing at least two of the following microorganisms in a mass ratio: Bacillus, Trichoderma, and Pseudomonas.

[0018] The Bacillus species is at least one of Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus natto, and Bacillus coagulans; the Trichoderma species is at least one of Trichoderma harzianum, Trichoderma viride, Trichoderma longibranchii, and Trichoderma echinosporum; and the Pseudomonas species is at least one of Pseudomonas fluorescens, Pseudomonas aeruginosa, and Pseudomonas putida.

[0019] The total effective viable count of the functional microbial composition is greater than 2.0 × 10⁻⁶. 9 CFU / g. The effective viable count of Bacillus spp. was 1.0 × 10⁻⁶. 9 -5.0×10 9 The effective spore count of Trichoderma species is 1.0 × 10⁻⁶ CFU / g. 8 -1.0×10 9 The effective viable count of Pseudomonas spp. was 5.0 × 10⁻⁶ CFU / g. 8 -2.0×10 9 CFU / g.

[0020] The nutrient carrier is obtained by mixing an organic matrix and an inorganic mineral matrix in a mass ratio of 3-7:7-3. The organic matrix is ​​at least one of well-rotted farmyard manure, humic acid, amino acid fermentation broth, molasses, yeast extract powder, and soybean meal powder. The carbon-nitrogen ratio of the organic matrix is ​​15-30:1, and the water content is less than 20%. The inorganic mineral matrix is ​​at least one of diatomaceous earth, zeolite powder, bentonite, vermiculite, kaolin, and attapulgite.

[0021] The additives include a dispersant and a water-retaining agent, with a mass ratio of dispersant:water-retaining agent = 1-3:1-2. The dispersant is at least one of sodium lignosulfonate, sodium dodecylbenzenesulfonate, sodium dodecyl sulfate, polyethylene glycol, polyvinylpyrrolidone, and sodium carboxymethyl cellulose. The water-retaining agent is at least one of polyacrylamide, sodium polyacrylate, sodium alginate, sodium carboxymethyl cellulose, xanthan gum, and guar gum.

[0022] In one embodiment, the adjuvant further includes a pH adjuster and a biocontrol synergist. The pH adjuster is at least one of potassium dihydrogen phosphate, dipotassium hydrogen phosphate, calcium carbonate, calcium oxide, citric acid, and humic acid, added to maintain the pH value of the inoculant at 5.5-7.5. The biocontrol synergist is at least one of chitin, chitosan, salicylic acid, methyl jasmonic acid, oligosaccharide, and acetylsalicylic acid.

[0023] The microbial agent also includes trace element components, the total mass of which accounts for 0.5%-2% of the total mass of the microbial agent. The trace element components are obtained by mixing at least two of zinc sulfate, ferrous sulfate, manganese sulfate, borax, sodium molybdate and copper sulfate in a mass ratio of 1-2:1-2:0.5-1.

[0024] In specific implementation, this invention includes: separately culturing each functional microorganism, collecting bacterial cells or spores, and drying to obtain raw powder; mixing organic matrix and inorganic mineral matrix in a certain proportion and drying to obtain nutrient carrier; mixing the nutrient carrier, adjuvants, and trace element components evenly first, then adding the functional microbial composition, and finally adding an aqueous solution of pH adjuster and biocontrol synergist, stirring evenly, granulating, and drying at low temperature to obtain the finished microbial agent.

[0025] In one embodiment: The functional microbial composition consists of a compound of Bacillus and Trichoderma in a mass ratio of 6:4. The selected Bacillus species is Bacillus subtilis, with an effective viable count of 3.5 × 10⁻⁶. 9 CFU / g. The selected Trichoderma species was *Trichoderma harzianum*, with an effective spore count of 6.0 × 10⁻⁶. 8 The total effective viable count of the functional microbial composition was 2.3 × 10⁻⁶ CFU / g. 9 CFU / g, dosage is 12 portions; The organic matrix of the nutrient carrier is a mixture of well-rotted farmyard manure and molasses at a mass ratio of 5:1, the inorganic mineral matrix is ​​a mixture of diatomaceous earth and zeolite powder at a mass ratio of 2:1, and the organic matrix and inorganic mineral matrix are mixed at a mass ratio of 5:5, with a dosage of 75 parts. Sodium lignosulfonate was selected as the dispersant in the additives, polyacrylamide was selected as the water-retaining agent, the mass ratio of dispersant to water-retaining agent was 2:1, the amount of basic additives was 3 parts, potassium dihydrogen phosphate was selected as the pH adjuster, the amount was 1.5 parts, and chitosan was selected as the biocontrol synergist, the amount was 1 part. The trace element components were prepared by mixing zinc sulfate, ferrous sulfate, and borax in a mass ratio of 2:2:1, with a dosage of 1 part. The finished product had a pH of 6.8 and a total effective viable bacteria count of 2.1 × 10⁻⁶. 9 CFU / g.

[0026] In one embodiment: The functional microbial composition is a ternary compound of Bacillus, Trichoderma, and Pseudomonas, in a mass ratio of 5:2:3. The selected Bacillus species is *Bacillus amyloliquefaciens*, with an effective viable count of 4.0 × 10⁻⁶. 9 CFU / g, Trichoderma species selected: *Trichoderma viride*, effective spore count 5.0 × 10⁻⁶ 8 CFU / g, for Pseudomonas spp., fluorescent Pseudomonas was selected, with an effective viable count of 1.2 × 10⁻⁶. 9 The total effective viable count of the functional microbial composition was 2.8 × 10⁻⁶ CFU / g. 9 CFU / g, dosage is 10 parts; The organic matrix of the nutrient carrier is a mixture of humic acid and yeast extract powder in a mass ratio of 3:1, the inorganic mineral matrix is ​​a mixture of bentonite and vermiculite in a mass ratio of 1:1, and the organic matrix and inorganic mineral matrix are mixed in a mass ratio of 4:6, with a dosage of 80 parts. Sodium dodecylbenzenesulfonate was selected as the dispersant and sodium alginate as the water-retaining agent. The mass ratio of dispersant to water-retaining agent was 3:2. The dosage of the basic additive was 2.5 parts. Humic acid was selected as the pH adjuster and the dosage was 1 part. Chitin and salicylic acid were mixed in a mass ratio of 1:1 and the dosage was 1.5 parts. The trace element components were prepared by mixing manganese sulfate, borax, and sodium molybdate in a mass ratio of 2:1:0.5, with a dosage of 0.8 parts. The finished product had a pH of 6.2 and a total effective viable bacteria count of 2.5 × 10⁻⁶. 9 CFU / g.

[0027] The contents not described in detail in this specification are existing technologies known to those skilled in the art.

[0028] The working principle of this invention: After the compound antibacterial agent of this invention is applied to the soil, Bacillus, Trichoderma and Pseudomonas in the functional microbial composition rapidly colonize and reproduce in the carbon source, nitrogen source and mineral microporous environment provided by the nutrient carrier, and inhibit soil-borne pathogens through multiple mechanisms such as competing for ecological niches, secreting antibacterial substances and inducing plant systemic resistance. The organic matter and inorganic mineral compound carrier continuously releases nutrients and retains water and fertilizer, prolongs microbial activity, the dispersant promotes the uniform dispersion of the inoculant in the soil, the water-retaining agent maintains a moist microenvironment in the rhizosphere, the pH regulator maintains a suitable acidity and alkalinity, and the biocontrol synergist activates the plant's defense response. Micronutrient supplementation promotes microbial metabolism and plant growth, forming a green prevention and control system with synergistic interaction between microorganisms, plants, and soil.

[0029] The present invention and its embodiments have been described above, and such description is not restrictive. In conclusion, if those skilled in the art are inspired by this description and design similar structures and embodiments without departing from the spirit of the invention, such designs should fall within the protection scope of the present invention.

Claims

1. A compound microbial agent for preventing soil-borne diseases in greenhouse fruits and vegetables, characterized in that: This includes functional microbial compositions, nutrient carriers, and adjuvants; The proportions of each component are as follows: 5-15 parts of functional microbial composition, 60-85 parts of nutrient carrier, and 0.5-5 parts of adjuvant. The functional microbial composition is obtained by mixing at least two of the following microorganisms in a certain mass ratio: Bacillus, Trichoderma, and Pseudomonas. The nutrient carrier is obtained by mixing an organic matrix and an inorganic mineral matrix in a mass ratio of 3-7:7-3. The additives include dispersants and water-retaining agents, with a mass ratio of dispersant:water-retaining agent = 1-3:1-2.

2. The compound antifungal agent for soil-borne diseases of greenhouse fruits and vegetables according to claim 1, characterized in that: The Bacillus species mentioned are at least one of Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus natto, and Bacillus coagulans. The Trichoderma species mentioned are at least one of Trichoderma harzianum, Trichoderma viride, Trichoderma longibranchii, and Trichoderma echinosporum. The Pseudomonas species mentioned are at least one of Pseudomonas fluorescens, Pseudomonas aeruginosa, and Pseudomonas putida.

3. The compound antifungal agent for soil-borne diseases of greenhouse fruits and vegetables according to claim 2, characterized in that: The total effective viable count of the functional microbial composition is greater than 2.0 × 10⁻⁶. 9 CFU / g; in: The effective viable count of Bacillus spp. is 1.0 × 10⁻⁶. 9 -5.0×10 9 CFU / g; The effective spore count of Trichoderma species is 1.0 × 10⁻⁶. 8 -1.0×10 9 CFU / g; The effective viable count of Pseudomonas spp. was 5.0 × 10⁻⁶. 8 -2.0×10 9 CFU / g.

4. The compound antifungal agent for soil-borne diseases of greenhouse fruits and vegetables according to claim 1, characterized in that: The organic matrix is ​​at least one of well-rotted farmyard manure, humic acid, amino acid fermentation liquid, molasses, yeast extract powder and soybean meal powder. The inorganic mineral matrix is ​​at least one of diatomaceous earth, zeolite powder, bentonite, vermiculite, kaolin, and attapulgite. The organic matrix has a carbon-to-nitrogen ratio of 15-30:1 and a water content of less than 20%.

5. The compound antifungal agent for soil-borne diseases of greenhouse fruits and vegetables according to claim 1, characterized in that: The dispersant is at least one of sodium lignosulfonate, sodium dodecylbenzenesulfonate, sodium dodecyl sulfate, polyethylene glycol, polyvinylpyrrolidone, and sodium carboxymethyl cellulose. The water-retaining agent is at least one of polyacrylamide, sodium polyacrylate, sodium alginate, sodium carboxymethyl cellulose, xanthan gum, and guar gum.

6. The compound microbial agent for preventing soil-borne diseases of greenhouse fruits and vegetables according to claim 1, characterized in that: The adjuvants also include pH adjusters and biocontrol synergists; The pH adjuster is at least one of potassium dihydrogen phosphate, dipotassium hydrogen phosphate, calcium carbonate, calcium oxide, citric acid, and humic acid, and is added to maintain the pH value of the bacterial agent at 5.5-7.

5. The biocontrol synergistic substance is at least one of chitin, chitosan, salicylic acid, methyl jasmonic acid, oligosaccharide and acetylsalicylic acid.

7. The compound microbial agent for preventing soil-borne diseases of greenhouse fruits and vegetables according to claim 1, characterized in that: The microbial agent also includes trace element components, the total mass of which accounts for 0.5%-2% of the total mass of the microbial agent.

8. The compound microbial agent for preventing soil-borne diseases of greenhouse fruits and vegetables according to claim 7, characterized in that: The trace element component is obtained by mixing at least two of zinc sulfate, ferrous sulfate, manganese sulfate, borax, sodium molybdate and copper sulfate in a mass ratio of 1-2:1-2:0.5-1.