Production method and application of composite raw material insect-repelling and disease-preventing agricultural enzyme

Abstract

The application relates to the technical field of enzyme preparation, and discloses a production method of a composite raw material insect-repelling and disease-preventing agricultural enzyme, which comprises the following steps: S1, preparing raw materials, which comprises the following raw materials in parts by weight: 6-12 parts of sugarcane molasses, 40-60 parts of water and 38-46 parts of composite raw materials; the composite raw materials are composed of mugwort, garlic, karerawan fruit and tobacco leaves; S2, crushing and mixing: fresh mugwort is crushed to 3-5 cm, fresh garlic and garlic sprouts are crushed to 3-5 cm, fresh karerawan fruit and karerawan tree leaves are crushed, and fresh tobacco leaves are crushed to 3-5 cm, and then all the solid raw materials are mixed; S3, mixing and fermentation: the mixed raw materials, the sugarcane molasses and the water are poured into a fermentation tank for fermentation. The four kinds of plant raw materials with insect-repelling and disease-preventing activities are compounded and fermented for the first time, allicin, nicotine, karerawan fruit and mugwort essential oil form a "multi-target action system", which can not only repel pests but also inhibit bacteria, and solves the problems of narrow application range and weak pertinence of single-component enzyme.

Description

Technical Field

[0001] This invention relates to the field of enzyme preparation technology, specifically to a method and application for producing a compound raw material insect repellent and disease-preventing agricultural enzyme. Background Technology

[0002] In the field of agricultural pest and disease control, there are already single-component enzymes (such as garlic enzymes and tobacco enzymes) or chemical pesticide alternatives. They mainly exert antibacterial or insecticidal effects through single active ingredients (such as allicin and nicotine) and are applied to the green control of diseases and pests in fruits, vegetables and grain crops.

[0003] Existing technological shortcomings: Single-component enzymes are highly targeted but have a narrow range of applications (e.g., they are only effective against a certain type of pest), making it difficult to cope with multiple pest and disease outbreaks. Some active enzyme components are easily decomposed, have a short duration of effect, and require frequent application, increasing planting costs. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention provides a method and application for producing compound raw material insect-repellent and disease-preventing agricultural enzymes. This solves the problems that single-component enzymes are highly targeted but have a narrow range of applications, making them difficult to cope with multiple pest and disease outbreaks; and that some enzyme active ingredients are easily decomposed, have a short duration of effect, and require frequent application, thus increasing planting costs.

[0005] To achieve the above objectives, the present invention provides the following technical solution: A method for producing a compound raw material insect-repellent and disease-preventing agricultural enzyme includes the following steps: S1: Prepare the raw materials, including the following parts by weight: 6-12 parts sugarcane molasses, 40-60 parts water, and 38-46 parts compound raw materials, which consist of mugwort, garlic, neem seeds, and tobacco leaves. S2: Grind and mix. Grind fresh mugwort to 3-5cm, fresh garlic and garlic sprouts to 3-5cm, crush fresh bitter lotus seeds and bitter neem leaves, and crush fresh tobacco leaves to 3-5cm. Then mix all solid ingredients. S3: Mixed fermentation. Pour the mixed raw materials, sugarcane molasses, and water into a fermentation tank. Control the temperature at 20-35℃ and seal for anaerobic fermentation for 180 days. S4: Filtration to remove impurities. After fermentation, filter to remove solid impurities. Take the filtrate and filter it through a filter screen to obtain a compound multifunctional agricultural enzyme.

[0006] As a further embodiment of the present invention, the proportions of mugwort, garlic, neem, and tobacco in the composite raw materials in S1 are equal.

[0007] Furthermore, in S2, the fresh tobacco leaves are crushed together, including the stems, leaves, seeds, and kernels.

[0008] Based on the aforementioned scheme, the fermentation process in S3 is stirred once during the first week, for 4-6 hours each time, followed by continuous sealed anaerobic fermentation.

[0009] Furthermore, the filtrate in S4 is filtered through an 80-100 mesh filter.

[0010] An application of a compound raw material insect repellent and disease prevention agricultural enzyme: After obtaining the agricultural enzyme, it is diluted with water at a ratio of 1:200-1:400 and sprayed during the crop growth period, once every 7-10 days, for 2-3 consecutive times, to inhibit the reproduction of pathogens and repel pests.

[0011] Compared with the prior art, the present invention provides a method and application for producing a compound raw material insect-repellent and disease-preventing agricultural enzyme, which has the following beneficial effects: 1. In this invention, the active ingredients such as eucalyptol and alkaloids in the four raw materials work synergistically to achieve a repellency rate of over 70% against pests such as aphids and spider mites, and an inhibition rate of over 60% against pathogens such as downy mildew and wilt. These ingredients can interfere with the nervous system of pests, inhibit the reproduction of pathogens, and replace chemical pesticides to achieve green prevention and control.

[0012] 2. In this invention, the active substances in the fermentation products can induce crops to produce disease-resistant proteins, enhance plant resistance to stress, reduce yield losses under adverse conditions, and promote crop root development, making the plant stems and vines thick and green, improving the crop's nutrient absorption efficiency, and ensuring stable growth.

[0013] 3. In this invention, the product is easily degradable, leaves no pesticide residue, and has no side effects on crops, soil, or the environment. It can be sprayed or irrigated after dilution and is suitable for various crops such as vegetables, fruit trees, and grains. It is easy to operate and can reduce the cost of pesticide use, helping to achieve "pesticide residue-free planting". The effective period after spraying is 7-10 days, which is 50% longer than the effective period of traditional single enzymes, reducing the application cost for growers.

[0014] 4. The invention features an innovative multifunctional synergistic formula: it is the first to use four plant raw materials with insect-repelling and disease-preventing activities in compound fermentation. Allicin, nicotine, bitter lotus extract, and mugwort essential oil form a "multi-target action system" that can both repel pests and inhibit pathogens, solving the problem of narrow applicability and weak targeting of single-component enzymes. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the steps in the production method of a compound raw material insect repellent and disease prevention agricultural enzyme proposed in this invention.

[0016] Figure 2 This is a comparison chart of the repellency rates of the control group and the compound enzyme group in the production method of a compound raw material insect repellent and disease prevention agricultural enzyme proposed in this invention.

[0017] Figure 3 This is a comparative table showing the insect-repelling effects of a compound raw material method for producing an insect-repelling and disease-preventing agricultural enzyme proposed in this invention.

[0018] Figure 4 This invention presents a comparative table of the disease prevention effects of a compound raw material method for producing an insect-repellent and disease-preventing agricultural enzyme.

[0019] Figure 5 This invention presents a comparative table of the effective duration of a compound raw material insect repellent and disease-preventing agricultural enzyme production method. Detailed Implementation

[0020] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention. Example 1

[0021] Reference Figure 1-5 A method for producing a compound raw material insect-repellent and disease-preventing agricultural enzyme includes the following steps: S1: Prepare the raw materials, including the following parts by weight: 8 parts sugarcane molasses, 52 parts water, and 40 parts compound raw materials. The compound raw materials consist of mugwort, garlic, neem seeds, and tobacco leaves. The proportions of mugwort, garlic, neem seeds, and tobacco leaves in the compound raw materials in S1 are the same. S2: Grind and mix. Grind fresh mugwort to 3cm, fresh garlic and garlic sprouts to 3cm, crush fresh bitter lotus seeds and bitter neem leaves, and crush whole fresh tobacco plants (stalks, leaves, seeds) to 3cm. Then mix all solid ingredients. S3: Mixed fermentation. Pour the mixed raw materials, sugarcane molasses, and water into a fermentation tank. Control the temperature at 22℃ and seal for anaerobic fermentation for 180 days. Stir once a week for 4 hours during the first week of fermentation in S3, and then continue sealed for anaerobic fermentation thereafter. S4: Filtration to remove impurities. After fermentation, filter to remove solid impurities. Take the filtrate and filter it through a filter screen to obtain a compound multifunctional agricultural enzyme. The filtrate in S4 is filtered through an 80-mesh filter screen.

[0022] Processing group An application of a compound raw material insect repellent and disease prevention agricultural enzyme: After obtaining the agricultural enzyme, it is diluted with water at a ratio of 1:200 and sprayed during the crop growth period, once every 7 days for 2 consecutive times, to inhibit the reproduction of pathogens and repel pests.

[0023] Control group: Garlic enzyme was applied normally. Example 2

[0024] Reference Figure 1-5 A method for producing a compound raw material insect-repellent and disease-preventing agricultural enzyme includes the following steps: S1: Prepare the raw materials, including the following parts by weight: 10 parts sugarcane molasses, 46 parts water, and 44 parts compound raw materials. The compound raw materials consist of mugwort, garlic, neem seeds, and tobacco leaves. The proportions of mugwort, garlic, neem seeds, and tobacco leaves in the compound raw materials in S1 are the same. S2: Grind and mix. Grind fresh mugwort to 5cm, fresh garlic and garlic sprouts to 5cm, crush fresh bitter lotus seeds and bitter neem leaves, and crush fresh tobacco leaves (seeds, leaves, seeds) to 5cm. Then mix all solid ingredients. S3: Mixed fermentation. Pour the mixed raw materials, sugarcane molasses, and water into a fermentation tank. Control the temperature at 35℃ and seal for anaerobic fermentation for 180 days. Stir once a week for 6 hours during the first week of fermentation in S3, and then continue sealed for anaerobic fermentation thereafter. S4: Filtration to remove impurities. After fermentation, filter to remove solid impurities. Take the filtrate and filter it through a filter screen to obtain a compound multifunctional agricultural enzyme. The filtrate in S4 is filtered through a 00 mesh filter screen.

[0025] Processing group An application of a compound raw material insect repellent and disease prevention agricultural enzyme: After obtaining the agricultural enzyme, it is diluted with water at a ratio of 1:400 and sprayed during the crop growth period, once every 10 days for 3 consecutive times, to inhibit the reproduction of pathogens and repel pests.

[0026] Control group: Normal application of tobacco enzymes.

[0027] In the description herein, it should be noted that relational terms such as "first" and "second" are used merely to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0028] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A method for producing a compound raw material insect-repellent and disease-preventing agricultural enzyme, characterized in that, Includes the following steps: S1: Prepare the raw materials, including the following parts by weight: 6-12 parts sugarcane molasses, 40-60 parts water, and 38-46 parts compound raw materials, which consist of mugwort, garlic, neem seeds, and tobacco leaves. S2: Grind and mix. Grind fresh mugwort to 3-5cm, fresh garlic and garlic sprouts to 3-5cm, crush fresh bitter lotus seeds and bitter neem leaves, and crush fresh tobacco leaves to 3-5cm. Then mix all solid ingredients. S3: Mixed fermentation. Pour the mixed raw materials, sugarcane molasses, and water into a fermentation tank. Control the temperature at 20-35℃ and seal for anaerobic fermentation for 180 days. S4: Filtration to remove impurities. After fermentation, filter to remove solid impurities. Take the filtrate and filter it through a filter screen to obtain a compound multifunctional agricultural enzyme.

2. The method for producing a compound raw material insect-repellent and disease-preventing agricultural enzyme according to claim 1, characterized in that, In S1, the proportions of mugwort, garlic, neem, and tobacco in the composite raw materials are equal.

3. The method for producing a compound raw material insect-repellent and disease-preventing agricultural enzyme according to claim 1, characterized in that, In S2, the fresh tobacco leaves are crushed together, including the stems, leaves, seeds, and kernels.

4. The method for producing a compound raw material insect-repellent and disease-preventing agricultural enzyme according to claim 1, characterized in that, In the S3 fermentation process, the mixture is stirred once during the first week, for 4-6 hours each time, and then continuously sealed for anaerobic fermentation.

5. The method for producing a compound raw material insect-repellent and disease-preventing agricultural enzyme according to claim 1, characterized in that, The filtrate in S4 is filtered through an 80-100 mesh filter.

6. The application of a compound raw material insect-repelling and disease-preventing agricultural enzyme, characterized in that, After obtaining the agricultural enzyme, dilute it with water at a ratio of 1:200-1:400 and spray it during the crop growth period. Spray once every 7-10 days for 2-3 consecutive times to inhibit the reproduction of pathogens and repel pests.

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