Water replacement method wet sesame residue fermented high-protein feed and preparation method thereof

Abstract

The present application relates to the technical field of feed processing, and discloses a kind of wet sesame dreg fermentation high-protein feed by water replacement method and a preparation method thereof.The method comprises the following steps: mechanically dehydrating wet sesame dregs generated in the production process of water replacement method ground sesame oil to a water content of 38% to 42%, then adding corn flour and bran to mix, to obtain fermentation material; inoculating bacillus subtilis, saccharomyces cerevisiae and enterococcus faecium into the fermentation material at the same time, and carrying out aerobic fermentation under the condition of aeration and turning, then sealing the material after aerobic fermentation to carry out anaerobic fermentation, to obtain the wet sesame dreg fermentation high-protein feed by water replacement method.The present application controls the water content of wet sesame dregs after dehydration by water replacement method, and combines the simultaneous inoculation of three kinds of microorganisms and the phased fermentation of aerobic fermentation first and anaerobic fermentation later, to improve the structure state and fermentation effect of fermentation material, promote protein degradation, and reduce the content of oxalic acid and tannin and other anti-nutritional factors.

Description

Technical Field

[0001] This application relates to the field of feed processing technology, specifically disclosing a water-displacement method for fermenting high-protein sesame residue into a wet feed and its preparation method. Background Technology

[0002] The water-displacement process for producing sesame oil generates a large amount of wet sesame residue, which has a crude protein content of over 40%, making it valuable as a protein feed ingredient. However, compared to sesame meal produced by pressing or leaching, wet sesame residue from water-displacement has a higher moisture content and residual oil content. The material is paste-like or mushy, characterized by strong viscosity, easy clumping, and poor aeration. It is not only prone to rancidity but also contains anti-nutritional factors such as oxalic acid and tannins, resulting in low digestibility and poor palatability when directly fed to the plant. Traditional methods of drying and direct use often suffer from high energy consumption, significant nutrient loss, and difficulty in effectively reducing anti-nutritional factors.

[0003] Currently, microbial solid-state fermentation has been used to improve the feed performance of sesame processing byproducts. Through microbial metabolism, macromolecules and anti-nutritional factors are degraded, producing metabolites such as organic acids. However, existing fermentation processes typically use sesame meal with low moisture content and a relatively loose structure as raw material. When applied to wet sesame residue processed by water displacement, the material easily forms dense clumps, resulting in insufficient internal aeration. Using either a single aerated fermentation method or a completely sealed fermentation method makes it difficult to meet the oxygen requirements of different microorganisms, affecting protein degradation and the removal of anti-nutritional factors. Therefore, how to improve fermentation conditions based on the material characteristics of wet sesame residue processed by water displacement and coordinate the roles of different microorganisms in the fermentation process has become a technical problem that needs to be solved for the feed utilization of wet sesame residue processed by water displacement. Summary of the Invention

[0004] In view of this, this application provides a method for preparing high-protein feed from wet sesame residue fermented by water displacement. By controlling the moisture content of the wet sesame residue after dehydration, and by combining simultaneous inoculation of three microorganisms with a phased fermentation process of first aerobic and then anaerobic, this application improves the structural state and fermentation effect of the fermented material, promotes protein degradation, and reduces the content of anti-nutritional factors such as oxalic acid and tannins.

[0005] The first aspect of this application provides a method for preparing high-protein feed from water-displaced wet sesame residue fermented using a water-displacement method, comprising the following steps: mechanically dehydrating the wet sesame residue produced during the water-displacement process of small-scale sesame oil production to a moisture content of 38% to 42%, then adding corn flour and wheat bran for mixing to obtain fermented material; simultaneously inoculating the fermented material with Bacillus subtilis, Saccharomyces cerevisiae, and Enterococcus faecalis, and carrying out aerobic fermentation under aeration and turning conditions; then sealing the aerobically fermented material for anaerobic fermentation to obtain high-protein feed from water-displaced wet sesame residue fermented using a water-displacement method.

[0006] Preferably, the mechanical dehydration is performed by pressure filtration of wet sesame residue with a water content of 65% to 75% and a residual oil content of 8% to 12% on a dry basis.

[0007] Preferably, the feed pressure of the filter press is 0.4~0.6MPa, the holding pressure is 0.8~1.0MPa, and the filter press time is 40~60min.

[0008] Preferably, based on the quality of the dehydrated wet sesame residue obtained by water displacement, the amount of corn flour and wheat bran added is 1% to 10%.

[0009] Preferably, based on the quality of the dehydrated wet sesame residue obtained by water displacement, the inoculation amount of Bacillus subtilis is 0.01%~0.05%, the inoculation amount of Saccharomyces cerevisiae is 0.02%~0.04%, and the inoculation amount of Enterococcus faecalis is 0.01%~0.2%.

[0010] Preferably, the inoculation amounts of Bacillus subtilis, Saccharomyces cerevisiae, and Enterococcus faecalis are 0.03%, 0.03%, and 0.1%, respectively.

[0011] Preferably, the Bacillus subtilis, the Saccharomyces cerevisiae, and the Enterococcus faecium are Bacillus subtilis CICC 10073, Saccharomyces cerevisiae CICC 31077, and Enterococcus faecium ATCC 19434, respectively.

[0012] Preferably, the aerobic fermentation environment temperature is 34~36℃ and the time is 24h.

[0013] Preferably, during the aerobic fermentation process, the material layer thickness is 30-40cm, natural ventilation is used, and the turning frequency is once every 6 hours; the anaerobic fermentation time is 48 hours.

[0014] The second aspect of this application provides a water-displacement method for fermenting high-protein feed from wet sesame residue, which is prepared by the aforementioned method for preparing high-protein feed from wet sesame residue fermented with water.

[0015] This application does not involve directly fermenting ordinary sesame meal with compound microorganisms. Instead, it addresses the characteristics of water-displaced wet sesame residue, such as high moisture content, high residual oil content, strong viscosity, easy clumping, and poor aeration. Mechanical dehydration and the addition of corn flour and wheat bran are used to adjust the material state, making it suitable for the aeration requirements of subsequent aerobic fermentation. Based on this, Bacillus subtilis, Saccharomyces cerevisiae, and Enterococcus faecium are simultaneously inoculated. Aerobic fermentation conditions are established through aeration and turning, followed by a sealed transition to anaerobic fermentation conditions, allowing the three microorganisms to play their respective roles at different fermentation stages. Compared to fully sealed fermentation, this application can increase the proportion of acid-soluble proteins in the fermentation product, reduce oxalic acid and tannin content, and improve the texture and odor of the fermentation product. By coordinating the control of the material state of water-displaced wet sesame residue with the transition of fermentation environment stages, the efficiency of protein degradation and removal of anti-nutritional factors is improved, thereby enhancing the feed value of water-displaced wet sesame residue. Detailed Implementation

[0016] The technical solutions in the embodiments of this application will be clearly and completely described below with reference to the embodiments of this application. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application. In addition, it should be understood that the specific implementation methods described herein are only for illustration and explanation of this application and are not intended to limit this application.

[0017] In the description of this application, the term "comprising" means "including but not limited to". The terms first, second, third, etc. are used merely as illustrative purposes and do not impose numerical requirements or establish an order.

[0018] In this application, "and / or" describes the relationship between related objects, indicating that three relationships can exist. For example, A and / or B can represent: A existing alone, A and B existing simultaneously, or B existing alone. A and B can be singular or plural.

[0019] In this application, "at least one" means one or more, and "more than one" means two or more. "One or more", "at least one of the following", or similar expressions refer to any combination of these items, including any combination of single or multiple items. For example, "at least one of a, b, or c", or "at least one of a, b, and c" can both mean: a, b, c, ab (i.e., a and b), ac, bc, or abc, where a, b, and c can be single or multiple.

[0020] Various embodiments of this application may exist in the form of a range; it should be understood that the description in the form of a range is merely for convenience and brevity and should not be construed as a rigid limitation on the scope of this application; therefore, it should be considered that the range description has specifically disclosed all possible sub-ranges and single numerical values ​​within that range. For example, it should be considered that the range description from 1 to 6 has specifically disclosed sub-ranges such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6, etc., and single numbers within the range, such as 1, 2, 3, 4, 5, and 6, regardless of the range. Furthermore, whenever a numerical range is referred to herein, it means including any referenced number (fraction or integer) within the referred range.

[0021] The present application will be specifically described below through specific embodiments. The following embodiments are only some embodiments of the present application and are not intended to limit the present application.

[0022] In this application, "water-displacement wet sesame residue" refers to the wet byproduct generated during the water-displacement process of small-scale sesame oil production, distinguished from sesame meal obtained by pressing or solvent extraction. The residual oil content of water-displacement wet sesame residue is characterized by its crude fat content on a dry basis. The raw material characteristics of water-displacement wet sesame residue and sesame meal obtained by pressing or solvent extraction are shown in Table 1.

[0023] Table 1. Raw material characteristics of wet sesame residue from water displacement method and sesame meal from pressing or leaching method.

[0024] Table 1 shows that the water-displacement method for wet sesame residue has a high moisture content and residual oil content. The protein, oil, and colloids are tightly bound, resulting in high material viscosity. Direct fermentation easily leads to the formation of dense clumps, hindering internal aeration. Furthermore, if fermentation is not properly controlled, the oil in the water-displacement wet sesame residue is prone to oxidation, producing a rancid odor and affecting the palatability of the resulting feed. Therefore, the fermentation conditions for sesame meal produced by conventional pressing or leaching methods cannot be directly applied.

[0025] In a first aspect, this application provides a method for preparing high-protein feed from fermented wet sesame residue using a water-displacement method, comprising the following steps: The wet sesame residue produced during the water-displacement process of small-batch sesame oil production is mechanically dehydrated to a moisture content of 38% to 42%, and then mixed with corn flour and wheat bran to obtain fermented material.

[0026] Bacillus subtilis, Saccharomyces cerevisiae, and Enterococcus faecalis were simultaneously inoculated into the fermentation material. Aerobic fermentation was carried out under aeration and turning conditions. The material after aerobic fermentation was then sealed for anaerobic fermentation to obtain high-protein feed from wet sesame residue fermented by water displacement method.

[0027] It should be noted that, in response to the characteristics of the wet sesame residue from the water-displacement method—high moisture content, high viscosity, and a tendency to clump—this application first mechanically dehydrates it to a moisture content of 38%–42% to reduce material viscosity and improve its structural state. Then, corn flour and wheat bran are added to adjust the carbon source composition and looseness of the material, resulting in a fermentation material with a state suitable for solid-state fermentation. Based on this, Bacillus subtilis, Saccharomyces cerevisiae, and Enterococcus faecalis are simultaneously inoculated into the fermentation material. Through initial aeration, turning, and subsequent sealing, the fermentation material is subjected to both aerobic and anaerobic fermentation conditions sequentially.

[0028] In some embodiments, mechanical dewatering includes pressure filtration of wet sesame residue with a water content of 65% to 75% and a residual oil content of 8% to 12% on a dry basis.

[0029] The feed pressure for the filter press is 0.4~0.6MPa, the holding pressure is 0.8~1.0MPa, and the filter press time is 40~60min. The wet sesame residue from the water-displacement method, after filter press, is a loose cake with a moisture content of 38%~42%. This moisture content refers to the moisture content of the wet sesame residue from the water-displacement method after mechanical dehydration, before the addition of corn flour and bran.

[0030] In one specific embodiment, the filter press is performed using a plate and frame hydraulic filter press of model XMYZ50 / 800-UB.

[0031] It should be noted that the moisture content of the wet sesame residue after mechanical dehydration via water displacement will affect the material's structural state and subsequent microbial fermentation process. When the moisture content is too high, the material becomes more viscous and prone to clumping, which in turn affects the oxygen supply during the aerobic fermentation stage; when the moisture content is too low, the material is too dry, which is not conducive to the growth and metabolism of microorganisms.

[0032] Specifically, to screen the suitable moisture content of mechanically dehydrated wet sesame residue produced by the water-displacement method, three experimental groups were set up with moisture contents of 35%, 40%, and 45%. For each group, 1000g of the same batch of dehydrated wet sesame residue was taken. Based on the weight of the dehydrated wet sesame residue, 5% corn flour, 8% wheat bran, 0.03% Bacillus subtilis inoculant, 0.03% Saccharomyces cerevisiae inoculant, and 0.1% Enterococcus faecalis inoculant were added and mixed thoroughly. Except for the moisture content, the other raw material ratios and fermentation conditions were the same for all experimental groups.

[0033] Each experimental group was placed in an environment of 35℃ for aerobic fermentation. Natural ventilation was used during the aerobic fermentation process, and the piles were turned over every 6 hours. After 24 hours of aerobic fermentation, samples were taken to test the viable count of Bacillus subtilis, coliform bacteria, and Salmonella in each experimental group.

[0034] In some embodiments, the viable count of Bacillus subtilis was determined using the selective plate count method. The test sample was serially diluted and plated onto Bacillus subtilis selective medium, incubated at 37°C for 24 hours, and then colony counting was performed. The results are expressed as CFU / g and calculated on a wet fermentation basis. Coliform bacteria were detected according to GB 4789.3-2016, and Salmonella was detected according to GB 4789.4-2024.

[0035] The test results showed that the material in the experimental group with a moisture content of 35% was relatively dry, and the viable count of Bacillus subtilis was [missing information]. CFU / g, coliforms and Salmonella were not detected; the material in the test group with a moisture content of 45% was more viscous, and the viable Bacillus subtilis count was [missing information]. CFU / g, coliform count: CFU / g, Salmonella not detected; Bacillus subtilis viable count reached [value missing] in the experimental group with 40% moisture content. CFU / g, coliform bacteria and Salmonella were not detected.

[0036] The results above show that among the three moisture content test groups set up in this experiment, the test group with a moisture content of 40% performed better. Considering the actual control precision of moisture content during the pressure filtration process, the moisture content of the wet sesame residue after mechanical dewatering by water displacement should be controlled at 38%~42%, preferably 40%.

[0037] In some embodiments, based on the mass of the dehydrated wet sesame residue obtained by water displacement, the amount of corn flour and wheat bran added is 1% to 10%, respectively.

[0038] Corn flour is used to provide fermentable carbohydrates and promote the growth of brewer's yeast and Enterococcus faecium. When the amount of corn flour added is less than 1%, the fermentable carbon source in the material is insufficient, and the microbial metabolism is slow; when the amount of corn flour added is more than 10%, the permeability of the material decreases, and clumping is likely to occur.

[0039] Wheat bran provides dietary fiber and growth factors, and improves the structure of fermented materials. When the amount of wheat bran added is less than 1%, its effect on improving the material structure is insufficient; when the amount of wheat bran added is more than 10%, it is easy to increase the crude fiber content of the resulting feed.

[0040] In a preferred embodiment of this application, based on the mass of the dehydrated wet sesame residue obtained by water displacement, the amount of corn flour added can be 5%, and the amount of wheat bran added can be 8%.

[0041] In some embodiments, based on the quality of the dehydrated wet sesame residue obtained by water displacement, the inoculation amount of Bacillus subtilis is 0.01%~0.05%, the inoculation amount of Saccharomyces cerevisiae is 0.02%~0.04%, and the inoculation amount of Enterococcus faecalis is 0.01%~0.2%.

[0042] In a preferred embodiment of this application, the inoculation amounts of Bacillus subtilis, Saccharomyces cerevisiae, and Enterococcus faecalis can be 0.03%, 0.03%, and 0.1%, respectively.

[0043] In some embodiments, Bacillus subtilis, Saccharomyces cerevisiae, and Enterococcus faecalis are Bacillus subtilis CICC10073, Saccharomyces cerevisiae CICC 31077, and Enterococcus faecalis ATCC 19434, respectively.

[0044] In one specific embodiment, the specific information of the three microbial agents is shown in Table 2.

[0045] Table 2. Detailed Information on Compound Microbial Agents

[0046] It should be noted that this application uses three types of microorganisms for simultaneous inoculation, and proceeds with aerobic fermentation and anaerobic fermentation in sequence, so that different microorganisms play their roles in the corresponding fermentation stages.

[0047] Specifically, during the aerobic fermentation stage, oxygen conditions are provided for the growth of Bacillus subtilis through aeration and turning, enabling Bacillus subtilis to grow and reproduce and produce proteases and amylases, which degrade macromolecular proteins and starches in the wet sesame residue from the water-displacement method, and provide small molecule nutrients for the subsequent fermentation of brewer's yeast and Enterococcus faecalis.

[0048] During the anaerobic fermentation stage, sealing the material after aerobic fermentation reduces the exchange between the fermentation material and the outside air, allowing the brewing yeast and Enterococcus faecalis to continue their metabolism using the available nutrients formed during the aerobic fermentation stage. This promotes a decrease in the pH of the fermentation system, which in turn facilitates the stable progress of anaerobic fermentation and reduces the content of anti-nutritional factors such as oxalic acid and tannins.

[0049] In some embodiments, the ambient temperature for both aerobic and anaerobic fermentation is 34~36℃. The aerobic fermentation time is 24h, and the anaerobic fermentation time is 48h.

[0050] In a preferred embodiment of this application, the ambient temperature for both aerobic and anaerobic fermentation is set to 35°C.

[0051] In some embodiments, during aerobic fermentation, the material layer thickness is 30-40 cm, natural ventilation is used, and the turning frequency is once every 6 hours.

[0052] In one specific embodiment, the process parameters for staged fermentation are shown in Table 3.

[0053] Table 3. Process parameters for staged fermentation

[0054] Specifically, after inoculating with the three microbial agents simultaneously, the fermentation material is first placed under aeration for aerobic fermentation, and the fermentation material is turned at a set frequency. After the aerobic fermentation is completed, the fermentation material is sealed and transferred to the anaerobic fermentation stage. The total time for aerobic and anaerobic fermentation is 72 hours.

[0055] In a preferred embodiment of this application, after fermentation, a high-protein feed made from wet sesame residue fermented using the wet water displacement method is obtained. Depending on the product form and storage and transportation requirements, the obtained fermented material can also be dried, pulverized, and packaged.

[0056] Secondly, this application provides a water-displacement method for fermenting high-protein feed from wet sesame residue, which is prepared by the water-displacement method for fermenting high-protein feed from wet sesame residue.

[0057] Example 1: This example provides a method for preparing high-protein feed from fermented wet sesame residue using a water-displacement method, specifically including the following steps: 500 kg of wet sesame residue from the water-displacement process of sesame oil production was collected. The moisture content, crude protein content, crude fat content, crude fiber content, oxalic acid content, tannin content, and pH of the wet sesame residue were tested. Specifically, moisture content was determined according to GB 5009.3-2016, crude protein content according to GB / T 6432-2018, crude fat content according to GB 5009.6-2016, crude fiber content according to GB / T 6434-2022, and tannin content according to GB / T 27985-2011. Oxalic acid content was determined using high-performance liquid chromatography (HPLC), and pH was determined using a calibrated pH meter. The test results are shown in Table 4.

[0058] Table 4. Raw material test results of wet sesame residue processed by water displacement method

[0059] A plate and frame hydraulic filter press (model XMYZ50 / 800-UB) was used to filter wet sesame residue produced by the water-displacement method. The feed pressure was 0.4~0.6 MPa, the holding pressure was 0.8~1.0 MPa, and the filtration time was 50 min. During filtration, some water was discharged, and a small amount of fine solids were discharged with the filtrate. After filtration, approximately 251 kg of dewatered wet sesame residue was obtained, with a moisture content of approximately 40.0%, and the material was in a loose cake-like form. Based on the material mass and moisture content before and after filtration, the dry matter mass of the wet sesame residue before filtration was approximately 158.5 kg, and the dry matter mass after filtration was approximately 150.6 kg, with a dry matter loss rate of approximately 5.0%.

[0060] 12.55 kg of corn flour and 20.08 kg of wheat bran were added to approximately 251 kg of dehydrated wet sesame residue produced by the water-displacement method, and mixed evenly to obtain fermented material. The amount of corn flour added was 5% and the amount of wheat bran added was 8% based on the mass of the dehydrated wet sesame residue produced by the water-displacement method.

[0061] Add 0.0753 kg of Bacillus subtilis inoculant, 0.0753 kg of Saccharomyces cerevisiae inoculant, and 0.251 kg of Enterococcus faecium inoculant to the fermentation material and mix thoroughly. Based on the mass of the dehydrated wet sesame residue from the water-displacement method, the addition amounts of Bacillus subtilis inoculant, Saccharomyces cerevisiae inoculant, and Enterococcus faecium inoculant were 0.03%, 0.03%, and 0.1%, respectively. The Bacillus subtilis strain was Bacillus subtilis CICC 10073, the Saccharomyces cerevisiae strain was Saccharomyces cerevisiae CICC 31077, and the Enterococcus faecium strain was Enterococcus faecium ATCC19434.

[0062] The inoculated fermentation material was placed into a plastic fermentation tank with ventilation holes, and the material layer thickness was controlled at 30-40 cm. Aerobic fermentation was carried out at an ambient temperature of 35℃. Natural ventilation was used during the aerobic fermentation process, and the material was turned over every 6 hours.

[0063] After 24 hours of aerobic fermentation, the material was not transferred and was directly sealed inside the original fermentation tank: first, a PE film was applied to cover the surface of the material and extended to the inside of the tank wall; then, the vent holes on the fermentation tank wall were sealed with PE film; finally, the entire PE film was used to cover the upper edge of the fermentation tank and the edges of the PE film were compacted to make the fermentation tank a sealed state; then, anaerobic fermentation was carried out at an ambient temperature of 35℃ for 48 hours to obtain high-protein feed from wet sesame residue fermented by water displacement method.

[0064] The resulting high-protein feed from water-displaced wet sesame residue fermentation is light brown in color, loose in texture, has a fermented aroma, a moisture content of approximately 38.2%, and a pH of 4.3.

[0065] Comparative Example 1: This comparative example provides a method for preparing wet sesame residue fermented feed using the water displacement method.

[0066] The water-displacement method for wet sesame residue, the pressure filtration method, the amount of corn flour and wheat bran added, the types and amounts of the three microbial agents, the fermentation temperature, and the total fermentation time used in this comparative example are all the same as in Example 1.

[0067] The difference between this comparative example and Example 1 is that after the three microbial agents were inoculated onto the fermentation material, a PE film was attached to cover the surface of the material to block the air vents on the fermentation tank wall, and the upper edge of the fermentation tank was covered with a PE film to form a sealed state. The fermentation was carried out in a sealed environment at 35°C for 72 hours, without ventilation or turning the pile during the fermentation process.

[0068] After fermentation, the resulting fermented material is dark brown with some clumps, has a slightly rancid smell, a moisture content of 39.5%, and a pH of 5.1.

[0069] The test results of the examples and comparative examples were tested on the raw material, wet sesame residue obtained by water displacement method in Example 1, the high-protein feed fermented from wet sesame residue obtained by water displacement method in Example 1, and the fermented material obtained in Comparative Example 1. Specifically, the dry basis crude protein content was determined according to GB / T 6432-2018, the proportion of acid-soluble protein to crude protein was determined according to NY / T 3801-2020, the tannin content was determined according to GB / T 27985-2011, the oxalic acid content was determined by high performance liquid chromatography, and the pH was determined using a calibrated pH meter. The test results are shown in Table 5.

[0070] Table 5. Test results of wet sesame residue obtained by water displacement method and materials obtained by different fermentation methods.

[0071] As shown in Table 5, under the same conditions of raw material, corn flour and bran addition amount, microbial agent type and inoculation amount, fermentation environment temperature and total fermentation time, compared with the fully sealed fermentation method used in Comparative Example 1, Example 1 adopted a staged fermentation method of aeration and turning in the early stage and sealing in the later stage. The resulting fermented material had higher dry basis crude protein content and acid-soluble protein ratio, and lower oxalic acid content, tannin content and pH.

[0072] Furthermore, the fermented material obtained in Example 1 was light brown, loose in texture, and had a fermented aroma; the fermented material obtained in Comparative Example 1 was dark brown, with localized clumps and a slightly rancid odor. These results indicate that the staged fermentation method employed in this application is beneficial for promoting protein degradation, reducing the content of anti-nutritional factors such as oxalic acid and tannins, and simultaneously improving the texture and odor of the fermented material.

[0073] The above are all preferred embodiments of the present invention and are not intended to limit the scope of protection of the present invention. Therefore, all equivalent modifications, equivalent improvements or equivalent substitutions made based on the formula, process, structure and principle of the present invention should be included within the scope of protection of the present invention.

Claims

1. A method for preparing high-protein feed from fermented wet sesame residue using a water-displacement method, characterized in that, Includes the following steps: The wet sesame residue produced during the water-displacement process of small-batch sesame oil production is mechanically dehydrated to a moisture content of 38% to 42%, and then mixed with corn flour and wheat bran to obtain fermented material. Bacillus subtilis, Saccharomyces cerevisiae, and Enterococcus faecalis are simultaneously inoculated into the fermentation material. Aerobic fermentation is carried out under aeration and turning conditions. The material after aerobic fermentation is then sealed for anaerobic fermentation to obtain high-protein feed from wet sesame residue fermented by water displacement method.

2. The method for preparing high-protein feed from wet sesame residue fermented using the water displacement method according to claim 1, characterized in that, The mechanical dehydration involves pressing wet sesame residue with a water-displacement method, having a moisture content of 65% to 75% and a residual oil content of 8% to 12% on a dry basis.

3. The method for preparing high-protein feed from wet sesame residue fermented using the water displacement method according to claim 2, characterized in that, The feed pressure of the filter press is 0.4~0.6MPa, the holding pressure is 0.8~1.0MPa, and the filter press time is 40~60min.

4. The method for preparing high-protein feed from wet sesame residue fermented using the water displacement method according to claim 1, characterized in that, Based on the quality of the dehydrated wet sesame residue obtained by water displacement, the amount of corn flour and wheat bran added is 1% to 10%.

5. The method for preparing high-protein feed from wet sesame residue fermented using the water displacement method according to claim 1, characterized in that, Based on the quality of the dehydrated wet sesame residue obtained by water displacement, the inoculation amount of Bacillus subtilis is 0.01%~0.05%, the inoculation amount of Saccharomyces cerevisiae is 0.02%~0.04%, and the inoculation amount of Enterococcus faecalis is 0.01%~0.2%.

6. The method for preparing high-protein feed from wet sesame residue fermented using the water displacement method according to claim 5, characterized in that, The inoculation amounts of Bacillus subtilis, Saccharomyces cerevisiae, and Enterococcus faecalis were 0.03%, 0.03%, and 0.1%, respectively.

7. The method for preparing high-protein feed from wet sesame residue fermented using the water displacement method according to claim 5, characterized in that, The Bacillus subtilis, the Saccharomyces cerevisiae, and the Enterococcus faecium are Bacillus subtilis CICC 10073, Saccharomyces cerevisiae CICC 31077, and Enterococcus faecium ATCC 19434, respectively.

8. The method for preparing high-protein feed from wet sesame residue fermented using the water displacement method according to claim 1, characterized in that, The aerobic fermentation environment is 34~36℃ for 24 hours.

9. The method for preparing high-protein feed from wet sesame residue fermented using the water displacement method according to claim 8, characterized in that, During the aerobic fermentation process, the material layer thickness is 30-40cm, natural ventilation is used, and the turning frequency is once every 6 hours; the anaerobic fermentation time is 48 hours.

10. A high-protein feed made from fermented wet sesame residue using a water-displacement method, characterized in that, It is prepared by the water-displacement method for fermenting wet sesame residue into high-protein feed as described in any one of claims 1 to 9.

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