Slow-release compound feed additive for livestock and poultry stress resistance and preparation method and application thereof

Abstract

The application belongs to the technical field of feed additives, and discloses a slow-release type livestock and poultry anti-stress compound feed additive as well as a preparation method and application thereof, and solves the problems of single efficacy, easy loss of activity and limited application scenarios of the existing products. The raw materials of the application include, by weight, 1-5 parts of star anise extract, 1-5 parts of enzymatic yeast polysaccharide, 1-5 parts of glucose oxidase, 1-5 parts of compound vitamin, 1-5 parts of taurine and 1-5 parts of butyric acid clostridium. The slow-release type livestock and poultry anti-stress compound feed additive provided by the application has an addition amount of 500 mg / kg in a full-price daily ration of livestock and poultry, and is suitable for chickens, ducks, geese, pigs and cattle. The application constructs a multi-target point synergistic regulation system, simultaneously realizes whole-body stress protection, intestinal barrier repair, immune enhancement and growth promotion of livestock and poultry, completely retains active substances through a low-temperature slow-release processing technology, widens the suitable product category of livestock and poultry, and comprehensively improves the comprehensive income of breeding.

Description

Technical Field

[0001] This invention belongs to the field of feed additive technology, and particularly relates to a slow-release compound feed additive for livestock and poultry to combat stress, its preparation method and application. Background Technology

[0002] In modern large-scale livestock and poultry farming, various factors such as nutritional fluctuations, environmental temperature differences, sustained high temperatures, relocation and capture, long-distance transportation, high-density feeding, frequent vaccinations, and peripartum cycles in dairy cows can all induce stress responses in livestock and poultry. When livestock and poultry are under stress, their redox balance is disrupted, inflammatory factors are abnormally secreted in large quantities, the intestinal mucosa is damaged, the flora is disordered, and the development of immune organs is inhibited. The direct manifestations are decreased feed intake, slower growth rate, increased feed conversion ratio, and increased mortality rate. At the same time, muscles are prone to oxidative deterioration, significantly reducing farming profits.

[0003] Currently, mainstream anti-stress products on the market have several shortcomings: single vitamin C and succinate products have a single mechanism of action, which can only temporarily and mildly relieve stress symptoms and cannot simultaneously address multiple needs such as anti-oxidation, intestinal repair, immune enhancement, and growth promotion; some probiotic compound products lack functional nutritional ingredients that target and regulate systemic stress, and have limited effects on improving the long-distance transport of beef cattle, the peripartum period of dairy cows, and the continuous heat stress of poultry. In addition, there is competition for oxygen in the intestine between aerobic and anaerobic microorganisms in the system, which reduces the colonization efficiency of live bacteria; conventional powder and high-temperature granulation products lack sustained-release carriers, and the active substances dissolve rapidly in the stomach and are easily decomposed by gastric acid. Glucose oxidase and anaerobic butyric acid clostridium are both heat-sensitive materials, and high-temperature processing will cause a large amount of activity loss, resulting in a serious decline in efficacy over the shelf life of the finished product; most compound products do not specifically replenish vitamins lost under stress, and it is difficult to continuously repair oxidative damage in the body under long-term stress conditions. Summary of the Invention

[0004] One objective of this invention is to provide a slow-release compound feed additive for livestock and poultry to combat stress, effectively solving the problems of existing products having single efficacy, easy loss of activity, and limited application scenarios.

[0005] To solve the above-mentioned technical problems, the technical solution adopted by the present invention is as follows: A slow-release compound feed additive for livestock and poultry to combat stress is prepared from the following raw materials in parts by weight: 1-5 parts star anise extract, 1-5 parts enzymatically hydrolyzed yeast polysaccharide, 1-5 parts glucose oxidase, 1-5 parts compound vitamins, 1-5 parts taurine, and 1-5 parts Clostridium butyricum.

[0006] Furthermore, it is prepared from the following raw materials in parts by weight: 3 parts star anise extract, 2 parts enzymatically hydrolyzed yeast polysaccharide, 2 parts glucose oxidase, 3 parts compound vitamins, 2 parts taurine, and 2 parts Clostridium butyricum.

[0007] Furthermore, it is prepared from the following raw materials in parts by weight: 4 parts star anise extract, 3 parts enzymatically hydrolyzed yeast polysaccharide, 3 parts glucose oxidase, 2 parts compound vitamins, 3 parts taurine, and 3 parts Clostridium butyricum.

[0008] Furthermore, it is prepared from the following raw materials in parts by weight: 3 parts star anise extract, 3 parts enzymatically hydrolyzed yeast polysaccharide, 2 parts glucose oxidase, 2 parts compound vitamins, 2 parts taurine, and 3 parts Clostridium butyricum.

[0009] Furthermore, it is prepared from the following raw materials in parts by weight: 4 parts star anise extract, 2 parts enzymatically hydrolyzed yeast polysaccharide, 3 parts glucose oxidase, 3 parts compound vitamins, 3 parts taurine, and 2 parts Clostridium butyricum.

[0010] Furthermore, the content of trans-anisole in star anise extract is ≥3800mg / kg, and the compound vitamins include vitamins A, C, E and B vitamins; the dosage form of the slow-release livestock and poultry anti-stress compound feed additive is powder, premix or 20-40 mesh slow-release granules.

[0011] Another objective of this invention is to provide a method for preparing the slow-release compound feed additive for livestock and poultry to combat stress as described in the above embodiments. The method involves weighing each raw material according to the corresponding weight proportions, mixing them at 15-25°C, then adding a slow-release skeletal excipient obtained by compounding maltodextrin and corn starch at a mass ratio of 1:1. The compounding and slow-release granulation are completed at a low temperature of 40-50°C to produce granules with a particle size of 20-40 mesh. These granules are then graded and sieved to obtain the slow-release compound feed additive for livestock and poultry to combat stress.

[0012] Another object of the present invention is to provide the application of the slow-release livestock and poultry anti-stress compound feed additive described in the above embodiments or the slow-release livestock and poultry anti-stress compound feed additive prepared by the preparation method described in the above embodiments, for the preparation of livestock and poultry anti-stress compound feed.

[0013] Furthermore, the dosage of slow-release compound feed additives for combating stress in livestock and poultry is 500-800 mg / kg in complete daily rations for livestock and poultry, and is applicable to chickens, ducks, geese, pigs, and cattle.

[0014] Compared with the prior art, the beneficial technical effects of the present invention are: (1) Synergistic effect of multiple target components to comprehensively cover various stress damages: Six types of functional raw materials are scientifically compounded, while taking into account the body's overall metabolic regulation, intestinal flora balance, mucosal immunity enhancement, antioxidant and anti-inflammatory effects, and stress nutrition supplementation; it can stably play an improving role in various breeding stress scenarios such as heat stress, regrouping and transportation, weaning, perinatal, and nutritional deficiency, with richer conditioning dimensions.

[0015] (2) Exclusive intestinal anaerobic microenvironment construction to improve the utilization efficiency of probiotics: Through glucose oxidation, the oxygen in the intestine is continuously consumed, creating a suitable living environment for Clostridium butyricum. It can ensure the colonization of intestinal probiotics without the need for aerobic spores, avoiding the problem of decreased activity caused by oxygen competition among the bacterial community, and the intestinal repair effect is stable and long-lasting.

[0016] (3) Low-temperature sustained-release integrated process, double protection of heat-sensitive active substances: The entire production process is controlled in the mild range of 40-50℃ to avoid the inactivation of enzymes and live bacteria caused by high temperature; the sustained-release porous skeleton isolates gastric acid and realizes long-term drug release in the intestine. Compared with conventional processing methods, the retention rate of effective ingredients is increased by 25%, the intestinal action time is extended by 30%, and the raw material utilization efficiency is significantly improved.

[0017] (4) It is applicable to a wide range of livestock and poultry species, covering poultry and ruminants. Its efficacy is suitable for chickens, ducks, geese, pigs, beef cattle and dairy cattle. It can solve the problems of high-density heat stress in poultry, weaning stress in piglets, long-distance transportation of cattle and sheep and peripartum stress in dairy cattle, and fill the gap that single preparations cannot take into account the use of poultry and ruminants.

[0018] (5) Simultaneously improve production performance and meat quality: continuously alleviate growth inhibition caused by stress, increase average daily weight gain and slaughter weight of livestock and poultry, reduce feed conversion ratio and mortality rate; at the same time, reduce oxidative damage to muscle, increase meat color redness, reduce muscle brightness, extend fresh meat shelf life, and improve slaughter output and product value.

[0019] (6) Green and safe for long-term large-scale feeding: All raw materials meet the relevant standards of the national feed raw materials and additives catalog, do not contain antibiotics or chemical anti-stress drugs, do not accumulate drugs or develop drug resistance, and can be added to young livestock, pregnant animals and lactating ruminants for a long time.

[0020] (7) Simple processing technology, suitable for industrial mass production: The mixing, granulation and screening processes are highly integrated, the production process is simplified, energy consumption is low, the process parameters are stable and controllable, continuous large-scale production can be realized, the production cost is controllable, and it is convenient for feed companies and large-scale farms to use in batches. Detailed Implementation

[0021] Example 1: A slow-release compound feed additive for livestock and poultry to combat stress, prepared from the following raw materials in parts by weight: 3 parts star anise extract, 2 parts enzymatically hydrolyzed yeast polysaccharide, 2 parts glucose oxidase, 3 parts compound vitamins, 2 parts taurine, and 2 parts Clostridium butyricum. The content of trans-anisole in the star anise extract is ≥3800 mg / kg.

[0022] The compound vitamins include vitamins A, C, E, and B vitamins. The compound vitamin used in this embodiment is Taigao Youtaiwei VM007.

[0023] The preparation method of the slow-release compound feed additive for livestock and poultry anti-stress provided in this embodiment is as follows: weigh each raw material according to the corresponding weight parts, put all raw materials into a mixer and stir at room temperature for 20 minutes to mix evenly; then add the slow-release skeleton excipient obtained by compounding maltodextrin and corn starch in a mass ratio of 1:1, and complete the compounding, slow-release granulation in one step at 45°C to make granules with a particle size of 30 mesh, and then sieve them through an 80 mesh sieve to obtain the slow-release compound feed additive for livestock and poultry anti-stress.

[0024] This embodiment innovates a low-temperature integrated sustained-release molding process, which differs from the traditional simple mixing and high-temperature granulation processing mode. After the mixed raw materials are combined with the sustained-release framework, a porous encapsulation structure is formed, simultaneously and stably encapsulating the fat-soluble star anise extract, water-soluble taurine, enzymatically hydrolyzed yeast polysaccharides, compound vitamins, heat-sensitive enzyme preparations, and anaerobic probiotics. After the granules reach the stomach, they slowly swell, reducing the damage of gastric acid to the active substances. After entering the intestines, the framework gradually degrades, and the effective ingredients are released at a uniform rate and continuously, prolonging the intestinal action time. Compared with conventional processing methods, the retention rate of effective ingredients can be increased by 25%, and the intestinal action time can be extended by 30%. The low-temperature processing throughout the process can significantly reduce the loss of glucose oxidase and Clostridium butyricum activity.

[0025] The efficacy and compatibility principle of each raw material in this embodiment are as follows: (1) Star anise extract: rich in high content of trans-anisole, which effectively removes reactive oxygen species generated by stress, inhibits the excessive release of pro-inflammatory factors such as TNF-α and IL-1β, and reduces the oxidative damage to the body tissues caused by various stresses from the source. (2) Taurine: a unique anti-stress functional amino acid that stabilizes cell membranes, downregulates stress cortisol, protects the liver, heart and other organs of livestock and poultry, improves metabolic disorders caused by long-distance transportation, high temperature and perinatal, and forms a dual antioxidant system with star anise extract. (3) Glucose oxidase: catalyzes the consumption of oxygen by intestinal glucose, continuously creates an anaerobic microenvironment suitable for the survival of Clostridium butyricum, reduces the proliferation of aerobic harmful bacteria in the intestine, and increases the colonization quantity and duration of action of Clostridium butyricum. (4) Clostridium butyricum: an anaerobic functional probiotic that continuously secretes butyric acid after colonizing the intestine, provides energy for intestinal epithelial cells, repairs the intestinal barrier damaged by stress, regulates the balance of intestinal flora, and reduces the entry of endotoxins into the blood circulation. (5) Enzymatic hydrolysis of yeast polysaccharides: Targets and activates intestinal mucosal immune cells, enhances the development of the thymus, spleen, and bursa of Fabricius, and reduces stress-induced immunosuppression. (6) Multivitamins: Replenishes the large amounts of vitamin C, vitamin E, and B vitamins consumed during stress, assists in the regeneration of antioxidant enzymes in the body, and makes up for the nutritional gap caused by an unbalanced diet.

[0026] The six raw materials have clear functions and complement each other, forming a complete regulatory chain of "systemic metabolic protection + natural anti-inflammatory and antioxidant + intestinal microenvironment regulation + intestinal barrier repair + mucosal immune activation + stress nutrition supply," which simultaneously alleviates various stress injuries in livestock and poultry through multiple pathways. The six raw materials work synergistically, and each functional pathway supports the other. The absence of any one raw material will significantly reduce the overall effect of anti-stress and growth promotion.

[0027] Example 2: The difference between this example and Example 1 is that the weight proportions of each raw material are as follows: 4 parts star anise extract, 3 parts enzymatically hydrolyzed yeast polysaccharide, 3 parts glucose oxidase, 2 parts compound vitamins, 3 parts taurine, and 3 parts Clostridium butyricum; the other raw material indicators and preparation parameters are consistent with those of Example 1.

[0028] Example 3: The difference between this example and Example 1 is that the weight proportions of each raw material are as follows: 3 parts star anise extract, 3 parts enzymatically hydrolyzed yeast polysaccharide, 2 parts glucose oxidase, 2 parts compound vitamins, 2 parts taurine, and 3 parts Clostridium butyricum; the other raw material indicators and preparation parameters are consistent with those of Example 1.

[0029] Example 4: The difference between this example and Example 1 is that the weight proportions of each raw material are as follows: 4 parts star anise extract, 2 parts enzymatically hydrolyzed yeast polysaccharide, 3 parts glucose oxidase, 3 parts compound vitamins, 3 parts taurine, and 2 parts Clostridium butyricum; the other raw material indicators and preparation parameters are consistent with those of Example 1.

[0030] The preferred weight ratios of the raw materials provided in Examples 1-4 can be flexibly selected according to the species being raised and the intensity of stress. The slow-release compound feed additives for livestock and poultry stress resistance provided in Examples 1-4 can be processed into slow-release granules, powders, and premixes, adaptable to different farm feeding equipment.

[0031] The slow-release compound feed additives for livestock and poultry provided in Examples 1-4 can be added to complete feeds for chickens, ducks, geese, pigs, beef cattle, and dairy cattle. The recommended addition amount is 500 mg / kg of diet. It can be used in regular nutritional diets as well as low-nutrition stress diets. Long-term feeding has no side effects and no risk of drug residues.

[0032] Experimental Example 1: This experimental example uses the slow-release compound feed additive for livestock and poultry anti-stress prepared in Example 1 (hereinafter referred to as "additive") as the test sample, and broilers as the breeding object to verify the application effect of the slow-release compound feed additive for livestock and poultry prepared in Example 1 in terms of antioxidant performance, immune function, production performance and slaughter performance.

[0033] 1. Experimental animals and grouping.

[0034] 1440 healthy AA broiler chickens aged 1 day were randomly divided into 4 treatment groups, with 9 replicates in each group and 40 chickens in each replicate.

[0035] Treatment 1 (positive control group): fed a diet with normal nutritional levels, without the additives prepared in Example 1.

[0036] Treatment 2 (negative control group): fed a low-nutrient diet (metabolizable energy reduced by 30 kcal / kg, crude protein reduced by 0.5%), without the additives prepared in Example 1.

[0037] Treatment 3: Feed a diet with normal nutritional levels, with the additive prepared in Example 1 added at 500 mg / kg of diet.

[0038] Treatment 4: Feed a diet with low nutritional levels (metabolizable energy reduced by 30 kcal / kg, crude protein reduced by 0.5%), and add 500 mg / kg of the additive prepared in Example 1 to the diet.

[0039] The experiment adopted a cage rearing model, divided into two feeding stages: broiler chick stage (0-21d) and broiler adult stage (22-42d). The experimental chickens had free access to feed and water, and the feeding environment conditions were kept consistent across all groups.

[0040] 2. Measurement indicators.

[0041] Feed intake and mortality were recorded throughout the process. Chickens were weighed on an empty stomach at 21 and 42 days of age to calculate average daily gain (ADG), average daily feed intake (ADFI), feed conversion ratio (F / G), and body weight (BW). Random samples were taken from each group at 21 and 42 days of age for slaughter to determine slaughter performance, including semi-eviscerated yield, fully eviscerated yield, breast muscle percentage, and leg muscle percentage. The weights of the thymus, spleen, and bursa of Fabricius were measured, and the immune organ index was calculated. Serum, liver, breast muscle, and leg muscle tissue were collected from the experimental chickens to detect inflammatory factors (TNF-α, IL-1β, IL-6, IL-10) and antioxidant indicators (SOD, GSH-Px, MDA).

[0042] 3. Experimental results.

[0043] 3.1 Production performance: As shown in Table 1, the experimental data for the whole period (1-42d) show that the addition of the additive prepared in Example 1 to the diet can significantly improve the average daily weight gain of broilers and reduce the feed conversion ratio (P<0.05); when faced with nutritional stress caused by low-nutrient diet, the additive prepared in Example 1 can effectively counteract the growth inhibition caused by nutrient reduction.

[0044] During the 22-42 day age period, the slaughter weight of broilers in treatment 3 was significantly increased, and the feed conversion ratio was significantly decreased; the additive prepared in Example 1 also showed a certain promoting effect on the early weight gain of broilers. Low-nutrient diets can cause broiler growth to slow down and feed utilization to decrease. Adding the additive prepared in Example 1 can effectively solve the above problems.

[0045] Table 1 Comparison of broiler production performance Note: In the table, SEM represents the standard error of the mean, P-value - additives represents the P-value between treatment 1 and treatment 3, P-value - nutrient level represents the P-value between treatment 1 and treatment 2, and P-value - interaction represents the P-value between treatment 2 and treatment 4.

[0046] 3.2 Slaughter performance: As shown in Table 2, the addition of the additive prepared in Example 1 significantly improved the leg muscle percentage of broilers at 21 days (P < 0.05) and showed a trend of increasing the semi-eviscerated percentage at 21 days; it had no adverse effects on any slaughter indicators at 42 days. There was no negative interaction between changes in dietary nutrient levels and the additive prepared in Example 1.

[0047] Table 2 Comparison of slaughter performance (%) Note: In the table, SEM represents the standard error of the mean, P-value - additives represents the P-value between treatment 1 and treatment 3, P-value - nutrient level represents the P-value between treatment 1 and treatment 2, and P-value - interaction represents the P-value between treatment 2 and treatment 4.

[0048] 3.3 Immune Function: As shown in Table 3, the addition of the additive prepared in Example 1 had no adverse effect on the organ index of broilers. As shown in Table 4, the addition of the additive prepared in Example 1 significantly downregulated the levels of inflammatory factors such as TNF-α, IL-6, IL-1β, and IL-10 in serum (P < 0.05), effectively inhibiting the body's inflammatory response, alleviating stress-induced immune disorders, and protecting the normal development of immune organs under nutritional stress conditions.

[0049] Table 3 Comparison of Organ Index (%) in Broilers Note: In the table, SEM represents the standard error of the mean, P-value - additives represents the P-value between treatment 1 and treatment 3, P-value - nutrient level represents the P-value between treatment 1 and treatment 2, and P-value - interaction represents the P-value between treatment 2 and treatment 4.

[0050] Table 4 Comparison of cytokines (ng / L) in broilers Note: In the table, SEM represents the standard error of the mean, P-value - additives represents the P-value between treatment 1 and treatment 3, P-value - nutrient level represents the P-value between treatment 1 and treatment 2, and P-value - interaction represents the P-value between treatment 2 and treatment 4.

[0051] 3.4 Antioxidant properties.

[0052] 3.4.1 Superoxide dismutase (SOD): As shown in Table 5, the additive prepared in Example 1 significantly increased the SOD activity of pectoral muscles, leg muscles and liver at 21 days and 42 days (P < 0.05), enhancing the body's ability to scavenge oxygen free radicals.

[0053] Table 5 Comparison of SOD activity (nmol / (mg·prot)) in broilers Note: In the table, SEM represents the standard error of the mean, P-value - additives represents the P-value between treatment 1 and treatment 3, P-value - nutrient level represents the P-value between treatment 1 and treatment 2, and P-value - interaction represents the P-value between treatment 2 and treatment 4.

[0054] 3.4.2 Malondialdehyde (MDA): MDA is a marker of oxidative damage. As shown in Table 6, the addition of the additive prepared in Example 1 significantly reduced the MDA content in the liver, pectoral muscles, and leg muscles (P < 0.05), thus alleviating tissue oxidative damage.

[0055] Table 6 Comparison of MDA content in broiler chickens Note: In the table, SEM represents the standard error of the mean, P-value - additives represents the P-value between treatment 1 and treatment 3, P-value - nutrient level represents the P-value between treatment 1 and treatment 2, and P-value - interaction represents the P-value between treatment 2 and treatment 4.

[0056] 3.4.3 Glutathione peroxidase (GSH-Px): As shown in Table 7, after adding the additive prepared in Example 1, the GSH-Px activity in the liver and leg muscles increased significantly (P<0.05), and the overall antioxidant system of the body was comprehensively enhanced. Table 7 Comparison of GSH-Px activity (nmol / (mg·prot)) in broiler chickens Note: In the table, SEM represents the standard error of the mean, P-value - additives represents the P-value between treatment 1 and treatment 3, P-value - nutrient level represents the P-value between treatment 1 and treatment 2, and P-value - interaction represents the P-value between treatment 2 and treatment 4.

[0057] 4. Experimental Summary: Adding the additive prepared in Example 1 to broiler diets effectively alleviates nutritional stress, enhances immune function, inhibits inflammatory responses, and comprehensively strengthens the body's antioxidant capacity; it also improves nutrient utilization, accelerates growth and weight gain, reduces feed conversion ratio, and improves slaughter performance; even under low-nutrient diet conditions, it can still exert stable effects, improving quality and efficiency, and enhancing the economic benefits of poultry farming. Experiments revealed that compared to ordinary powders and conventional granules, the sustained-release granule formulation prepared in Example 1 has a higher retention rate of active ingredients (approximately 25%) and a longer intestinal action time (approximately 30%), and at the same dosage, its anti-stress and growth-promoting effects are more stable.

[0058] Experimental Example 2: This experimental example uses the slow-release compound feed additive for livestock and poultry anti-stress prepared in Example 1 (hereinafter referred to as "additive") as the test sample, and uses meat ducks as the breeding object to verify the application effect of the additive prepared in Example 1 in terms of production performance, slaughter performance and meat quality improvement.

[0059] 1. Experimental animals and grouping.

[0060] 40,000 healthy 1-day-old ducks were randomly divided into two groups: (1) Control group: 20,000 ducks were fed a basic diet without the additive prepared in Example 1 and were slaughtered after 28 days. (2) Experimental group: 20,000 ducks were fed a basic diet with 500 mg / kg of the additive prepared in Example 1 and were slaughtered after 28 days.

[0061] All ducks are raised on wire mesh, and the diet composition and feeding environment of each group are completely consistent.

[0062] 2. Measurement indicators.

[0063] Record feed intake and survival rate, calculate daily weight gain and feed conversion ratio; determine semi-eviscerated rate, fully eviscerated rate, breast muscle rate, and leg muscle rate during slaughter; test breast muscle color (L value, a value, b value) and meat quality score.

[0064] 3. Experimental results.

[0065] 3.1 Production performance: As shown in Table 8, the comparison of simultaneous slaughter at 28 days showed that the slaughter weight of the experimental group increased by 1.6%, the daily weight gain increased by 1.7%, the feed intake decreased by 0.3%, the feed conversion ratio decreased by 1.9%, and the survival rate increased by 1.4%. The results indicate that the additive prepared in Example 1 can significantly improve the weight gain rate, feed utilization rate, and survival rate of meat ducks.

[0066] Table 8 Comparison of meat duck production performance 3.2 Slaughter performance: As shown in Table 9, there was no significant difference in the semi-eviscerated and fully eviscerated rates between the two groups of ducks (P>0.05); the breast muscle rate of the experimental group increased by 2.7% and the leg muscle rate increased by 2.2%, which has the effect of improving the meat production performance of ducks.

[0067] Table 9 Comparison of slaughter performance of meat ducks Note: SEM in the table represents the standard error of the mean.

[0068] 3.3 Meat quality indicators: As shown in Table 10, after adding the additive prepared in Example 1, the brightness value (L value) of the breast muscle of duck meat decreased significantly, the redness value (a value) increased significantly (P<0.05), and the overall meat quality score increased by 37.4%, effectively optimizing the appearance of meat color and improving the commercial quality of duck meat.

[0069] Table 10 Comparative Analysis of Color Values ​​of Duck Breast Muscle Note: SEM in the table represents the standard error of the mean.

[0070] 4. Experimental Summary: Adding the additive prepared in Example 1 to the diet of broiler ducks can stably increase daily weight gain, slaughter weight, and survival rate, while reducing the feed conversion ratio; it also slightly increases the output of breast and leg muscles. Simultaneously, it significantly improves meat color and optimizes meat quality, comprehensively enhancing the profitability and product quality of broiler duck farming. The slow-release formulation effectively avoids the problems of active ingredient inactivation and rapid metabolism, further improving the stability of the product's application in broiler duck farming.

[0071] Of course, the above description is not a limitation of the present invention, and the present invention is not limited to the examples given above. Any changes, modifications, additions or substitutions made by those skilled in the art within the scope of the present invention should also fall within the protection scope of the present invention.

Claims

1. A slow-release compound feed additive for livestock and poultry to combat stress, characterized in that, It is prepared from the following raw materials in parts by weight: 1-5 parts star anise extract, 1-5 parts enzymatically hydrolyzed yeast polysaccharide, 1-5 parts glucose oxidase, 1-5 parts compound vitamins, 1-5 parts taurine, and 1-5 parts Clostridium butyricum.

2. The slow-release compound feed additive for livestock and poultry to combat stress according to claim 1, characterized in that, It is prepared from the following raw materials in parts by weight: 3 parts star anise extract, 2 parts enzymatically hydrolyzed yeast polysaccharide, 2 parts glucose oxidase, 3 parts compound vitamins, 2 parts taurine, and 2 parts Clostridium butyricum.

3. The slow-release compound feed additive for livestock and poultry to combat stress according to claim 1, characterized in that, It is prepared from the following raw materials in parts by weight: 4 parts star anise extract, 3 parts enzymatically hydrolyzed yeast polysaccharide, 3 parts glucose oxidase, 2 parts compound vitamins, 3 parts taurine, and 3 parts Clostridium butyricum.

4. The slow-release compound feed additive for livestock and poultry to combat stress according to claim 1, characterized in that, It is prepared from the following raw materials in parts by weight: 3 parts star anise extract, 3 parts enzymatically hydrolyzed yeast polysaccharide, 2 parts glucose oxidase, 2 parts compound vitamins, 2 parts taurine, and 3 parts Clostridium butyricum.

5. The slow-release compound feed additive for livestock and poultry to combat stress according to claim 1, characterized in that, It is prepared from the following raw materials in parts by weight: 4 parts star anise extract, 2 parts enzymatically hydrolyzed yeast polysaccharide, 3 parts glucose oxidase, 3 parts compound vitamins, 3 parts taurine, and 2 parts Clostridium butyricum.

6. The slow-release compound feed additive for livestock and poultry anti-stress according to any one of claims 1-5, characterized in that, The content of trans-anisole in star anise extract is ≥3800mg / kg, and the complex vitamins include vitamins A, C, E and B vitamins; The dosage forms of slow-release compound feed additives for livestock and poultry to combat stress are powder, premix, or 20-40 mesh slow-release granules.

7. The method for preparing the slow-release compound feed additive for livestock and poultry anti-stress according to any one of claims 1-6, characterized in that, Weigh each ingredient according to the corresponding weight parts, stir and mix at 15-25℃; then add the slow-release skeleton excipient obtained by compounding maltodextrin and corn starch in a mass ratio of 1:1, and complete the compounding and slow-release granulation at a low temperature of 40-50℃ to make granules with a particle size of 20-40 mesh, and then grade and screen them to obtain the slow-release livestock and poultry anti-stress compound feed additive.

8. The application of the slow-release compound feed additive for livestock and poultry anti-stress as described in any one of claims 1-6, or the slow-release compound feed additive for livestock and poultry prepared by the preparation method of claim 7, characterized in that, Used to prepare stress-resistant compound feed for livestock and poultry.

9. The application according to claim 8, characterized in that, The dosage of slow-release compound feed additives for combating stress in livestock and poultry is 500-800 mg / kg in complete daily rations for livestock and poultry. Applicable livestock and poultry include chickens, ducks, geese, pigs, and cattle.

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