Multimodal rat application for MRI cryogenic coil non-invasive animal bed

Abstract

The application provides a non-invasive animal bed for a multi-modal rat applied to an MRI cryogenic coil, and relates to the field of magnetic resonance imaging.The non-invasive animal bed for the multi-modal rat applied to the MRI cryogenic coil comprises a front end base and a rear end base which are detachably connected; a head guiding piece, a tooth fixing seat and a head hoop are sequentially arranged on the front end base from front to back; the head guiding piece comprises two symmetrical head guiding seats, a nose pressing part is arranged above the two head guiding seats, a gap is formed between the tooth fixing seat and the front end base, and a tooth groove is arranged in the tooth fixing seat; two symmetrical forelimb through holes and two symmetrical forelimb fixing holes are further arranged on the front end base, the forelimb through holes are located behind the head hoop, and the forelimb fixing holes are located behind the forelimb through holes; two trunk fixing seats and two hindlimb fixing holes are arranged on the rear end base, and a trunk fixing hole is arranged in each trunk fixing seat.The application can stabilize the rat in a conscious state, thereby adapting to a conscious experiment.

Description

Technical Field

[0001] This invention relates to the field of magnetic resonance imaging, and in particular to a non-invasive animal bed for multimodal rats to be used in MRI cryocoil. Background Technology

[0002] In biomedical research, functional magnetic resonance imaging (fMRI) is a widely used tool for studying brain activity. Existing animal beds on the market are typically used for imaging studies under anesthesia, while those suitable for awake rats are often invasive devices used to study their neural activity in response to resting states and various stimuli. Furthermore, apart from standard animal beds with MRI cryocoagulation coils (which can only be used for anesthesia experiments and not for awake experiments), there are no animal beds on the market compatible with MRI cryocoagulation coils.

[0003] Most existing animal imaging bed designs, most similar to this invention, cannot simultaneously achieve both maintaining the animal's consciousness and non-invasive animal restraint. Two main technical solutions are currently available in the animal testing market. The first involves invasive surgery to install a fixation device on the animal's skull, which, when connected and fixed to the animal bed, controls brain movement. The second uses non-invasive animal bite bars and ear rods for positioning, combined with the continuous release of a specific dose of anesthetic gas, to effectively control brain movement. Some designs can fix the head of conscious animals for MRI, but a completely successful solution for cryo-MRI coil platforms is still lacking.

[0004] The drawbacks of existing techniques: Traditionally, most functional magnetic resonance imaging (fMRI) experiments in small animals are performed under anesthesia to minimize motion artifacts. However, the effects of anesthesia on the brain may result in results that do not accurately reflect neuronal activity in the conscious state, thus affecting the validity and generalizability of the research results. Among the proposed techniques, invasive MRI of conscious rats is a widely used approach, which can effectively reduce motion artifacts. However, the damage to the rat's brain caused by the experimenter and the success rate of the surgery are not optimistic. Inflammation and local proliferation in the rat's brain due to the experimenter's surgery can have uncontrollable effects on the MRI data. Furthermore, excessive experimental steps increase the time cost of the experiment.

[0005] Currently, there is a lack of suitable animal beds for non-invasive functional magnetic resonance imaging (fMRI) experiments on awake rats, especially when using cryogenic coils in small animal imaging systems. This limitation hinders researchers' ability to conduct precise scientific research under natural conditions. Summary of the Invention

[0006] To address the current challenge of conducting functional magnetic resonance imaging (fMRI) experiments on awake rats using non-invasive methods, this invention provides a non-invasive animal bed for multimodal rats to use with MRI cryocoil. This bed stabilizes rats in an awake state, enabling more realistic and natural brain function studies. It avoids neural activity deviations caused by anesthesia, prevents potential brain damage in rats that may occur with traditional methods, and is suitable for awake experiments.

[0007] The multimodal rat non-invasive animal bed for MRI cryocoil application provided by this invention includes a detachably connected front base and a rear base. The front base has a head guide, a tooth fixation seat, and a headband arranged sequentially from front to back, with the head guide being furthest from the rear base. The head guide includes two symmetrical head guide seats, with a nasal pressure or anesthesia mask positioned between them. A gap is formed between the tooth fixation seat and the front base, and the tooth fixation seat contains tooth grooves. The headband is arched and forms a gap with the front base. The front base also has two symmetrical forelimb through holes and two symmetrical forelimb fixation holes, with the forelimb through holes located behind the headband and the forelimb fixation holes located behind the forelimb through holes. The rear base has two trunk fixation seats and two hind limb fixation holes, with the trunk fixation seats detachably connected to the rear base. The hind limb fixation holes are furthest from the front base. The trunk fixation seats contain trunk fixation holes.

[0008] In one feasible embodiment of the present invention, the nasal pressure is replaced by an anesthesia mask, which is disposed between two head guide seats and is detachably connected to the head guide seats. The anesthesia mask is provided with a mask groove for placing the rat's nose, and the anesthesia mask is also provided with an anesthesia channel, which is connected to the mask groove.

[0009] In one feasible embodiment of the present invention, the front base is further provided with a shim, the shim being located below the headband, and the shim being used to elevate the rat's throat.

[0010] In one feasible embodiment of the present invention, the front end base is further provided with a first adaptation hole and a second adaptation hole, the first adaptation hole and the second adaptation hole being located at the front and rear of the shim block and adjacent to the shim block, respectively.

[0011] In one feasible embodiment of the present invention, the head guide seat includes a connecting part and an extension part connected in sequence. The connecting part is connected to the front end base, and the extension part extends away from the front end base. A buckle is provided on the end of the extension part away from the front end base, and the buckle is used to cooperate with the slot in the inner shell of the freezing coil.

[0012] In one feasible embodiment of the present invention, a transfer bed detachably connected to the rear base is also included, wherein the front base, the rear base and the transfer bed are arranged sequentially.

[0013] In one feasible embodiment of the present invention, the conveyor bed includes a conveyor plate and a conveyor housing disposed on a portion of the conveyor plate. The conveyor plate is detachably connected to the rear base, and the outer wall of the conveyor housing is adapted to fit against the inner wall of the inner housing of the refrigeration coil.

[0014] In one feasible embodiment of the present invention, an intermediate connecting plate is provided between the rear base and the conveyor bed, and the intermediate connecting plate is detachably connected to the rear base and the conveyor bed respectively.

[0015] In one feasible embodiment of the present invention, the head guide seat, the tooth fixation seat and the front base are all made of PEEK, the rear base is made of aluminum alloy, and the nose clamp and the headband are made of polymer resin.

[0016] This invention also provides a method for using a multimodal rat in a non-invasive animal bed for MRI cryocoil, comprising the following steps:

[0017] Step 1) Make the rat bite down on the tooth fixation seat, then fix the nasal pressure or anesthesia mask in sequence, and then fix the headband;

[0018] Step 2) Use a flexible fixation device to fix the rat's trunk through the trunk fixation hole;

[0019] Step 3) Use a flexible fixation device to fix the rat's hind limb through the hind limb fixation hole;

[0020] Step 4) Pass the rat's forelimb through the forelimb through hole, and then use a flexible fastener through the forelimb fixation hole to fix the rat's forelimb.

[0021] The multimodal rat provided in this invention, applied to a non-invasive animal bed with an MRI cryocoil, has the following beneficial effects:

[0022] 1) This invention uses a combination of nasal pressure, dental fixation and headband, which can stabilize rats in a conscious state, thereby conducting more realistic and natural brain function studies, avoiding the deviation of neural activity caused by anesthesia, avoiding the brain damage that may occur in rats in traditional methods, and adapting to conscious experiments.

[0023] 2) This invention can reduce motion artifacts. The three-point head fixation of nasal pressure, dental fixation seat and headband can reduce motion artifacts and improve MRI imaging quality. Moreover, the device has wide applicability and can be used for rats of different sizes and weights. At the same time, it simplifies the operation process, reduces human error and improves experimental efficiency.

[0024] 3) This invention is multifunctional and flexible, and can be compatible with anesthesia experiments. Without changing other equipment, the nasal pressure can be replaced with an anesthesia mask, which can be adapted to research under anesthesia conditions, thus enhancing the multifunctionality and flexibility of the equipment.

[0025] 4) This invention provides experimental comfort. When fixing rats, innovative designs such as Velcro straps are used to reduce the stress and discomfort of the rats, ensuring a more natural physiological state and reliable experimental conditions.

[0026] 5) This invention has wide applicability, and its adjustable design makes the device suitable for rats of different sizes and weights, improving the versatility and convenience of the device.

[0027] 6) This invention can improve experimental efficiency. The standardized and automated data processing design simplifies the operation process, reduces manual steps and human error, and improves the efficiency of the entire experiment. Attached Figure Description

[0028] Figure 1 This is a schematic diagram of the overall structure of the nasal pressure device in this invention.

[0029] Figure 2 This is a schematic diagram of the front base and the rear base in this invention.

[0030] Figure 3 This refers to the front base and various components on the front base in this invention.

[0031] Figure 4 This is a schematic diagram of the dental fixation device in this invention.

[0032] Figure 5 This is a schematic diagram of the front base in this invention.

[0033] Figure 6 This is a schematic diagram of the overall structure of the present invention with an anesthesia mask installed.

[0034] Figure 7 This is a schematic diagram of the overall structure of the anesthesia mask in this invention.

[0035] Figure Labels

[0036] Front base 1

[0037] Forelimb through hole 11

[0038] Forelimb fixation hole 12

[0039] 13 raised blocks

[0040] First adaptation hole 14

[0041] Second adaptation hole 15

[0042] Rear Base 2

[0043] Hind limb fixation hole 21

[0044] Head guide 3

[0045] Head guide seat 31

[0046] Connecting part 31.1

[0047] Extension 31.2

[0048] 31.3 buckle

[0049] Dental Fixation 4

[0050] Tooth groove 41

[0051] Headband 5

[0052] Nasal pressure 6

[0053] Torso fixation 7

[0054] Torso fixation hole 71

[0055] 8 anesthesia masks

[0056] Mask groove 81

[0057] Anesthesia Channel 82

[0058] Conveyor Bed 9

[0059] Conveyor Plate 91

[0060] Conveyor housing 92

[0061] Intermediate connecting plate 93 Detailed Implementation

[0062] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention. In the description of the present invention, it should be noted that the terms "front and back," "above," "below," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the specification, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present invention. In addition, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0063] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0064] Furthermore, in the description of this invention, unless otherwise stated, "a plurality of" means two or more.

[0065] Example 1

[0066] This invention provides a non-invasive animal bed for multimodal rats to be used in MRI cryocoil, in conjunction with reference to [reference needed]. Figures 2-3 The device includes a detachably connected front base 1 and a rear base 2, which are typically plate-shaped. The front base 1 has, from front to back, a head guide 3, a tooth retainer 4, and a headband 5. The head guide 3 is furthest from the rear base 2. The head guide 3 is used to secure part of the rat's head, such as its mouth. The tooth retainer 4 is used to secure the rat's teeth. The headband 5 is used to wrap around the rat's head. (Continue reading...) Figures 2-3 The head guide 3 includes two symmetrical head guide seats 31. Symmetry can be understood as the two head guide seats 31 being symmetrical about the central axis of the front base 1. A nasal pressure 6 is provided between the two head guide seats 31. The nasal pressure 6 is typically detachably connected to the head guide seats 31, and a gap is left between the nasal pressure 6 and the front base 1. The nasal pressure 6 is used to fix the rat's nose. A gap is formed between the tooth fixation seat 4 and the front base 1. The tooth fixation seat 4 has tooth grooves 41, allowing the rat's upper jaw to pass over the top of the tooth fixation seat 4, and the rat's lower jaw to pass through the gap between the tooth fixation seat 4 and the front base 1, thus enabling the rat to bite the tooth fixation seat 4 and position its upper and lower incisors in the tooth grooves 41. The headband 5 is arched and forms a gap with the front base 1. Most of the rat's head is located in the gap between the headband 5 and the front base 1 and is secured by the headband 5. (Continue reading...) Figure 2 and Figure 3The front base 1 is also provided with two symmetrical forelimb passage holes 11 and two symmetrical forelimb fixing holes 12. The forelimb passage holes 11 are located behind the headband 5, and the forelimb fixing holes 12 are located behind the forelimb passage holes 11. The forelimb passage holes 11 are usually round holes, and their function is to allow the rat's forelimbs to pass through the front base 1 and thus be located on the other side of the front base 1. The forelimb fixing holes 12 are usually elliptical holes, and their function is to allow flexible fasteners, such as adhesive tape or Velcro, to pass through. The flexible fasteners can repeatedly pass through the forelimbs to fix and wrap around the rat's forelimbs, thereby fixing the rat's forelimbs to the front base 1. Continue reading Figure 2 and Figure 3 The rear base 2 is provided with two trunk fixation seats 7 and two hind limb fixation holes 21. The hind limb fixation holes 21 allow flexible fasteners, such as adhesive tape or Velcro, to pass through. These flexible fasteners can repeatedly pass through the forelimbs to fix and wrap around the rat's hind limbs, thereby fixing the rat's hind limbs to the front base 1. The trunk fixation seats 7 are detachably connected to the rear base 2. The hind limb fixation holes 21 are furthest from the front base 1. The trunk fixation seats 7 are provided with trunk fixation holes 71. The trunk fixation holes 71 allow flexible fasteners, such as adhesive tape or Velcro, to pass through. These flexible fasteners can repeatedly pass through the trunk fixation holes 71 and wrap around the rat's trunk, thereby fixing the rat's trunk to the rear base 2. Additionally, this invention is specifically designed to be compatible with the Bruker 9.4T MRI device. This invention uses a combination of nasal pressure 6, dental fixation seat 4, and headband 5 to stabilize rats in a conscious state, thereby enabling more realistic and natural brain function studies. It avoids neural activity deviations caused by anesthesia, avoids potential rat brain damage in traditional methods, and is suitable for conscious experiments.

[0067] As a supplementary explanation, the bottom of the front base and the bottom of the rear base are provided with connecting ribs, which can be connected by screws to realize the connection between the front base and the rear base.

[0068] In the non-invasive animal bed provided in the embodiments of the present invention, see... Figure 5 The front base 1 is also equipped with a shim 13, which is located below the headband 5. The shim 13 is used to elevate the rat's throat. In this invention, the rat is fixed in a prone position on a non-invasive animal bed. When the rat bites the tooth fixation seat 4, there is a certain angle between the rat's torso and its lower jaw, causing the rat's throat to be suspended in the air, with its center of gravity completely pressing on its teeth. This causes discomfort and puts a certain burden on the rat's body. Therefore, the shim 13 can effectively elevate the rat's throat, so that its throat is no longer suspended in the air, thereby relieving the rat's physical burden and reducing its discomfort.

[0069] In the non-invasive animal bed provided in the embodiments of the present invention, see... Figure 5 The front base 1 is further provided with a first adaptation hole 14 and a second adaptation hole 15, which are located at the front and rear of the raised block 13 and are close to the raised block 13, respectively. The first adaptation hole 14 is elliptical in shape, allowing the rat's chin to rest within it, preventing the rat's chin from being rubbed raw and causing inflammation. The second adaptation hole 15 is elliptical in shape, allowing the rat to maintain a natural posture and reducing the rat's stress. In a specific embodiment, the raised block 13 is U-shaped, and the first adaptation hole 14 is located at the notch of the U-shape.

[0070] In the non-invasive animal bed provided in the embodiments of the present invention, in conjunction with reference to Figure 2 and Figure 3 The head guide seat 31 includes a connecting portion 31.1 and an extension portion 31.2 connected in sequence. The connecting portion 31.1 is connected to the front base 1, and the extension portion 31.2 extends away from the front base 1. A buckle 31.3 is provided on the end of the extension portion 31.2 away from the front base 1. The buckle 31.3 is used to cooperate with a groove in the inner shell of the cryogenic coil. For illustration, the cryogenic coil typically includes an outer shell and an inner shell arranged concentrically with the outer shell and located inside the outer shell. The inner wall of the outer shell contacts the outer wall of the inner shell. A groove is provided in the inner wall of the inner shell, and the buckle 31.3 can match the groove to fix the head fixing seat, thereby preventing the non-invasive animal bed from rotating and moving radially in the inner shell.

[0071] In the non-invasive animal bed provided in the embodiments of the present invention, see... Figure 1 The system also includes a transfer bed 9 detachably connected to the rear base 2. The front base 1, rear base 2, and transfer bed 9 are arranged sequentially. The transfer bed 9 typically has a certain length to match the length of the freezing coil. Currently, an external push rod is needed to push the animal bed into the freezing coil to a certain depth. Therefore, the transfer bed 9 facilitates the worker in pushing the front base 1 and rear base 2 into the freezing coil to a certain depth. Furthermore, the transfer bed 9 includes a transfer plate 91 and a transfer housing 92 disposed on a portion of the transfer plate 91. The transfer plate 91 is detachably connected to the rear base 2. The outer wall of the transfer housing 92 is adapted to fit against the inner wall of the inner housing of the freezing coil to increase the friction between them, making it less likely for the front base 1 and rear base 2 to rotate when the transfer bed 9 is pushed.

[0072] In the non-invasive animal bed provided in the embodiments of the present invention, see... Figure 1An intermediate connecting plate 93 is provided between the rear base 2 and the conveyor bed 9, and the intermediate connecting plate 93 is detachably connected to the rear base 2 and the conveyor bed 9 respectively. In a specific embodiment, the rear base 2 is provided with a connecting terminal, which can be fixed by sequentially inserting screws into the connecting terminal, the intermediate connecting plate 93 and the conveyor bed 9.

[0073] In the non-invasive animal bed provided in this embodiment of the invention, the head guide seat 31, tooth retainer 4, and front base 1 are all made of PEEK, the rear base 2 is made of aluminum alloy, and the nasal pressure plate 6 and headband 5 are made of polymer resin. The nasal pressure plate 6 and headband 5 can be fabricated using SLA printing technology. The high precision and good surface quality of SLA printing technology are suitable for small parts with complex geometries, while also providing a non-magnetic material, making it suitable for MRI applications. PEEK material is an ideal choice due to its non-magnetic properties, chemical resistance, high-temperature stability, good biocompatibility, and mechanical strength, while the non-magnetic, lightweight, and corrosion-resistant properties of pure aluminum are suitable for processing components such as the transfer bed 9. Therefore, this invention possesses high precision and repeatability, using advanced materials and refined manufacturing processes to improve the accuracy and stability of the equipment, thereby enhancing the repeatability and reliability of experimental results.

[0074] This invention also provides a method for using multimodal rats in a non-invasive animal bed for MRI cryocoil, comprising the following steps:

[0075] Step 1) Make the rat bite the tooth fixation seat 4, then fix the nasal pressure 6 in sequence, and then fix the headband 5; furthermore, the nasal pressure 6 can be fixed to the head fixation seat with screws, and the headband 5 can also be fixed to the front base 1 with screws.

[0076] Step 2) Use a flexible fastener through the trunk fixation hole 71 to fix the rat's trunk. The flexible fastener can be adhesive tape or Velcro.

[0077] Step 3) Use a flexible fastener through the hind limb fixation hole 21 to fix the rat's hind limb. The flexible fastener can be adhesive tape or Velcro.

[0078] Step 4) Pass the rat's forelimb through the forelimb through hole 11, and then use a flexible fastener through the forelimb fixation hole 12 to fix the rat's forelimb. The flexible fastener can be adhesive tape or Velcro.

[0079] If you want to further secure the rat, you can also directly wrap the flexible fastener around the rat's torso to fix it to the front base 1 or the rear base 2.

[0080] Example 2: Compatible Anesthesia Experimental Protocol

[0081] In the non-invasive animal bed provided in the embodiments of the present invention, see... Figure 5 and Figure 6 In this embodiment, the nasal pressure 6 is replaced by an anesthesia mask 8. The anesthesia mask 8 is located between two head guide seats 31 and is detachably connected to the head guide seats 31. The anesthesia mask 8 has a mask groove 81 for placing the rat's nose, and an anesthesia channel 82 is also provided in the anesthesia mask 8, which is connected to the mask groove 81. In this embodiment, the nasal pressure 6 is replaced by an anesthesia mask 8, which is suitable for research under anesthesia conditions and enhances the versatility and flexibility of the equipment. The anesthesia experiment uses two-point restraint, using the dental fixation seat 4 and the headband 5 to control the head's degree of freedom. The anesthesia mask 8 is used to anesthetize the rat. The rat's torso is restrained by flexible fasteners such as adhesive tape or Velcro to accommodate different body shapes and reduce animal discomfort. The method of using the compatible anesthesia experiment is roughly the same as that of the embodiment one, the only difference being that after the rat is fixed, anesthetic gas needs to be introduced into the anesthesia mask 8 to anesthetize the rat.

[0082] As a supplementary explanation, the anesthesia channel 82 is typically used to introduce external anesthetic gas, and the anesthesia mask 8 is preferably made of polymer resin and can be prepared by SLA printing technology.

[0083] The usage method of this embodiment is the same as that of Embodiment 1. The nasal pressure 6 can be replaced by an anesthesia mask 8. In addition, if the anesthesia mask 8 is used for fixation, anesthesia gas needs to be introduced to anesthetize the rat after fixation.

[0084] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and substitutions can be made without departing from the technical principles of the present invention, and these improvements and substitutions should also be considered within the scope of protection of the present invention.

Claims

1. A non-invasive animal bed for multimodal rats used in MRI cryocoil, characterized in that: Includes a detachably connected front base (1) and a rear base (2); The front base (1) is provided with a head guide (3), a tooth fixation seat (4) and a headband (5) in sequence from front to back. The head guide (3) is furthest away from the rear base (2). The head guide (3) includes two symmetrical head guide seats (31). A nasal pressure (6) or anesthesia mask (8) is provided between the two head guide seats (31). A gap is formed between the tooth fixation seat (4) and the front base (1). A tooth groove (41) is provided in the tooth fixation seat (4). The headband (5) is arched and forms a gap with the front base (1). The front base (1) is also provided with two symmetrical forelimb through holes (11) and two symmetrical forelimb fixing holes (12). The forelimb through holes (11) are located after the headband (5), and the forelimb fixing holes (12) are located after the forelimb through holes (11). The rear base (2) is provided with two torso fixing seats (7) and two hind limb fixing holes (21). The torso fixing seats (7) are detachably connected to the rear base (2). The hind limb fixing holes (21) are furthest away from the front base (1). The torso fixing seats (7) are provided with torso fixing holes (71).

2. The non-invasive animal bed according to claim 1, characterized in that: The anesthesia mask (8) is provided with a mask groove (81) for placing the rat's nose, and the anesthesia mask (8) is also provided with an anesthesia channel (82), which is connected to the mask groove (81).

3. The non-invasive animal bed according to claim 1 or 2, characterized in that: The front base (1) is also provided with a shim (13), which is located below the headband (5) and is used to elevate the rat's throat.

4. The non-invasive animal bed according to claim 3, characterized in that: The front base (1) is also provided with a first adaptation hole (14) and a second adaptation hole (15), which are located at the front and rear of the shim block (13) and close to the shim block (13), respectively.

5. The non-invasive animal bed according to claim 1 or 2, characterized in that: The head guide seat (31) includes a connecting part (31.1) and an extension part (31.2) connected in sequence. The connecting part (31.1) is connected to the front base (1). The extension part (31.2) extends away from the front base (1). A buckle (31.3) is provided on the end of the extension part (31.2) away from the front base (1). The buckle (31.3) is used to cooperate with the slot in the inner shell of the freezing coil.

6. The non-invasive animal bed according to claim 1 or 2, characterized in that: It also includes a transfer bed (9) that is detachably connected to the rear base (2), wherein the front base (1), the rear base (2) and the transfer bed (9) are arranged in sequence.

7. The non-invasive animal bed according to claim 6, characterized in that: The conveyor bed (9) includes a conveyor plate (91) and a conveyor housing (92) disposed on a portion of the conveyor plate (91). The conveyor plate (91) is detachably connected to the rear base (2). The outer wall of the conveyor housing (92) is adapted to fit against the inner wall of the inner housing of the refrigeration coil.

8. The non-invasive animal bed according to claim 4, characterized in that: An intermediate connecting plate (93) is provided between the rear base (2) and the conveyor bed (9), and the intermediate connecting plate (93) is detachably connected to the rear base (2) and the conveyor bed (9) respectively.

9. The non-invasive animal bed according to claim 1, characterized in that: The head guide seat (31), the tooth fixation seat (4) and the front base (1) are all made of PEEK, the rear base (2) is made of aluminum alloy, and the nose pressure (6) and the headband (5) are made of polymer resin.

10. A method of using a multimodal rat as described in any one of claims 1 to 9 in a non-invasive animal bed for MRI cryocoil, comprising the following steps: Step 1) Make the rat bite the tooth fixation seat (4), then fix the nasal pressure (6) or the anesthesia mask (8) in sequence, and then fix the headband (5); Step 2) Use a flexible fixation device to fix the rat's trunk through the trunk fixation hole (71); Step 3) Use a flexible fixator to fix the rat's hind limb through the hind limb fixation hole (21); Step 4) Pass the rat’s forelimb through the forelimb through hole (11), and then use a flexible fastener through the forelimb fixation hole (12) to fix the rat’s forelimb.

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