An anti-aging composition, its preparation method and application

Abstract

The application provides an anti-aging composition and a preparation method and application thereof, and relates to the technical field of health products.The anti-aging composition provided by the application takes pyrroloquinoline quinone disodium salt (PQQ), beta-nicotinamide mononucleotide (NMN), ergothioneine, coenzyme Q10 and L-selenium-methyl selenocysteine as core active ingredients, and the five ingredients are combined to be used, so that the skin state and the body function recession can be improved in a synergistic manner, and the anti-aging effect is significantly better than that of simple compounding of single components or part of the components.The composition can be prepared into dosage forms such as tablets, capsules, granules or oral liquids, and the components can achieve a significant synergistic effect at a lower dosage.In addition, the anti-aging composition provided by the application has good stability and is suitable for preparing anti-aging health products.

Description

Technical Field

[0001] This invention relates to the field of health product technology, specifically to an anti-aging composition, its preparation method, and its application. Background Technology

[0002] As people become more health-conscious, the demand for anti-aging products is gradually increasing. However, current single anti-aging ingredients have limitations in their target areas and effects, making it difficult to comprehensively cover multiple core mechanisms in the aging process, such as cell damage, decreased energy metabolism, and oxidative stress imbalance, thus failing to meet users' needs for deep anti-aging.

[0003] Using β-nicotinamide mononucleotide alone only increases NAD + The existing technologies lack a comprehensive anti-aging composition that addresses the deficiencies in cellular energy management. This composition features a multi-component synergistic anti-aging effect, utilizing five components—NMN, PQQ, coenzyme Q10, ergothioneine, and L-selenomethylselenocysteine—to achieve a comprehensive, multi-level, and low-dose synergistic anti-aging effect across five aging-related pathways: energy metabolism, mitochondrial function, antioxidant defense, lipid peroxidation protection, and selenium-containing enzyme synthesis. The composition also addresses the limitations of existing technologies such as mitochondrial repair capacity, pyrroloquinoline quinone disodium salt (which only promotes mitochondrial regeneration and cannot regulate cellular energy), limited antioxidant capacity of coenzyme Q10, and susceptibility to oxidative degradation during storage. Summary of the Invention

[0004] In view of this, the purpose of the present invention is to provide an anti-aging composition comprising five core ingredients: disodium pyrroloquinoline quinone, β-nicotinamide mononucleotide, ergothioneine, coenzyme Q10 and L-seleno-methylselenocysteine, which can simultaneously improve skin aging and physical fatigue.

[0005] To achieve the above-mentioned objectives, the present invention provides the following technical solution: The present invention provides an anti-aging composition comprising the following components in parts by weight: 1-500 parts of disodium pyrroloquinoline quinone, 20-1000 parts of β-nicotinamide mononucleotide, 10-2000 parts of ergothioneine, 10-1500 parts of coenzyme Q10 and 0.015-0.6 parts of L-seleno-methylselenocysteine.

[0006] Preferably, the anti-aging composition comprises the following components in parts by weight: 10-30 parts of disodium pyrroloquinoline quinone, 200-400 parts of β-nicotinamide mononucleotide, 15-45 parts of ergothioneine, 10-40 parts of coenzyme Q10 and 0.03-0.07 parts of L-seleno-methylselenocysteine.

[0007] Preferably, the anti-aging composition further includes excipients; the excipients include at least one of the following components: 20-50 parts of microcrystalline cellulose, 5-50 parts of lactose, and 5-20 parts of magnesium stearate.

[0008] Preferably, the dosage form of the anti-aging composition is at least one of tablets, capsules, and oral liquids.

[0009] The present invention provides a method for preparing the anti-aging composition, comprising the following steps: mixing β-nicotinamide mononucleotide, disodium pyrroloquinoline quinone, ergothioneine, coenzyme Q10 and L-seleno-methylselenocysteine ​​according to the weight parts of the components, and the resulting mixed powder is the anti-aging composition.

[0010] Preferably, the mixed powder is filled into capsule shells, and the dosage form is capsules.

[0011] Preferably, the mixed powder is prepared into granules, tablets or oral liquid after being mixed with excipients.

[0012] This invention provides an application of the aforementioned anti-aging composition in the preparation of anti-aging products.

[0013] Preferably, the aging includes skin aging and / or decline in bodily functions.

[0014] Preferably, the anti-aging includes improving facial skin radiance and / or relieving physical fatigue associated with aging.

[0015] The anti-aging composition provided by this invention uses pyrrolizidine quinoline quinone disodium salt (PQQ), β-nicotinamide mononucleotide (NMN), ergothioneine, coenzyme Q10, and L-selenomethylselenocysteine ​​as its core active ingredients. Among them, NMN acts as an NAD+... + Its direct precursor can efficiently increase NAD in the body. +This formula activates energy metabolism pathways and provides essential coenzymes for cell repair. PQQ specifically promotes mitochondrial biogenesis, repairs damaged mitochondria, and enhances cellular energy supply. Ergothioneine, a natural and potent antioxidant, can scavenge excess reactive oxygen species in the body, reducing oxidative stress damage to cellular DNA and organelles. Coenzyme Q10 directly participates in electron transport in the mitochondrial electron transport chain and is an indispensable electron carrier in ATP synthesis, while also inhibiting lipid peroxidation and protecting the integrity of cell membrane structure. L-Selenomethylselenocysteine, as a highly efficient organic selenium source, is an important precursor for the synthesis of key selenium-containing enzymes such as glutathione peroxidase in the body. It can specifically catalyze the conversion of harmful peroxides into harmless substances by reduced glutathione, forming a dual scavenging system of water-soluble free radicals and peroxides with ergothioneine, synergistically enhancing cellular antioxidant defense capabilities. These five components act on five major aging-related physiological pathways: energy metabolism, mitochondrial function, antioxidant defense, peroxide scavenging, and cell repair and regeneration, forming a multi-target, multi-level synergistic anti-aging system that fully leverages the synergistic effects between the various active ingredients. Attached Figure Description

[0016] Figure 1 The results of the subjects' facial skin radiance and body feedback scores were obtained after 14 and 28 days of taking the anti-aging composition. Detailed Implementation

[0017] The present invention provides an anti-aging composition comprising the following components in parts by weight: 1-500 parts of disodium pyrroloquinoline quinone, 20-1000 parts of β-nicotinamide mononucleotide, 10-2000 parts of ergothioneine, 10-1500 parts of coenzyme Q10 and 0.015-0.6 parts of L-seleno-methylselenocysteine.

[0018] In this invention, the preferred weight percentage of the pyrroloquinoline quinone disodium salt (PQQ) is 5-300 parts, but it can be 10-100 parts, 10-30 parts, 15 parts, 20 parts, 25 parts, or 28 parts. PQQ specifically stimulates mitochondrial biogenesis, promotes mitochondrial regeneration and repair, enhances the energy supply capacity of the cell, and also possesses certain direct antioxidant activity. In the anti-aging composition of this invention, PQQ, as a mitochondrial function-enhancing component, is precisely matched to the metabolic requirements of NMN, ensuring that damaged mitochondria in aging cells are promptly cleared and replaced, thus providing NAD+. + The enhanced energy metabolism provides a physical carrier.

[0019] In this invention, the weight percentage of β-nicotinamide mononucleotide (NMN) is preferably 100-500 parts, but can be 100-600 parts, 200-400 parts, 240 parts, 280 parts, 320 parts, or 360 parts. The NMN serves as coenzyme I (NAD). + A key precursor to NAD+, its core function is to efficiently increase the body's NAD+ levels. + This level activates the longevity protein Sirtuins, improves cellular energy metabolism, repairs damaged DNA, and fundamentally slows down the cellular aging process. In the anti-aging composition described in this invention, NMN serves as a core energy metabolism regulating component, providing fundamental efficacy for the entire anti-aging system. It addresses the limitations of other components such as PQQ and coenzyme Q10 in NAD+ regulation. + The problem of insufficient regulatory capacity in metabolic pathways needs to be addressed to ensure that cells have enough energy for subsequent repair.

[0020] In this invention, the preferred weight percentage of ergothioneine is 10-1000 parts, but it can be 10-100 parts, 15-45 parts, 25 parts, 30 parts, 35 parts, or 40 parts. Ergothioneine, as a natural and potent antioxidant, can be specifically transported to sites of high oxidative stress, such as mitochondria, via a dedicated transporter protein, effectively scavenging reactive oxygen species and protecting cellular DNA and organelles from oxidative damage. In the anti-aging composition of this invention, ergothioneine serves as the core of the antioxidant defense, compensating for the shortcomings of NMN and PQQ in directly scavenging free radicals, reducing secondary damage to newly formed mitochondria and DNA caused by oxidative stress. Furthermore, ergothioneine possesses extremely high chemical stability, reducing the degradation of NMN and Coenzyme Q10 in the formulation due to oxidation during production and storage. Simultaneously, by scavenging free radicals in vivo, it provides a stable internal environment for the efficacy of NMN and PQQ, ensuring the longevity of the overall anti-aging effect of the composition.

[0021] In this invention, the preferred weight percentage of coenzyme Q10 is 10-750 parts, but it can be 10-100 parts, 10-40 parts, or even 20, 25, 30, or 35 parts. Coenzyme Q10 is an essential hydrogen carrier for ATP synthesis in the mitochondrial electron transport chain and can also inhibit lipid peroxidation on the cell membrane, protecting the structural integrity of blood vessels and skin cell membranes. In the anti-aging composition of this invention, coenzyme Q10, as an energy synthesis aid and lipid antioxidant component, can assist PQQ in enhancing mitochondrial electron transport efficiency. Simultaneously, utilizing its lipid solubility, it exerts its antioxidant effect in the cell membrane lipid layer, which is inaccessible to the water-soluble antioxidant ergothione. Furthermore, its reduced form (panthenol) can be recycled and regenerated by other antioxidant systems in the body, forming a comprehensive water-lipid biphasic protective network.

[0022] In this invention, the weight percentage of L-seleno-methylselenocysteine ​​is preferably 0.015-0.1 parts, but can be 0.03-0.07 parts, or 0.004 parts, 0.005 parts, or 0.006 parts. L-seleno-methylselenocysteine ​​serves as an organic selenium source. Selenium is the active center of glutathione peroxidase (GPx), possessing a strong ability to scavenge free radicals; it also promotes cell repair and regeneration, maintains homeostasis by regulating metabolism, and reduces the accumulation of harmful waste. In the anti-aging composition of this invention, L-seleno-methylselenocysteine ​​acts as a metabolic regulator and regenerative repair component, repairing damaged tissues and removing metabolic waste, thus forming a perfect closed loop in the anti-aging system.

[0023] In this invention, the anti-aging composition preferably further includes excipients. The excipients preferably include at least one of the following components: 20-50 parts microcrystalline cellulose, 5-50 parts lactose, and 5-20 parts magnesium stearate. In this invention, the excipients are a key component in ensuring product molding, stability, and quality control.

[0024] In this invention, the microcrystalline cellulose can be 25 parts, 30 parts, 35 parts, or 40 parts. Microcrystalline cellulose is a multifunctional excipient widely used in pharmaceuticals, health foods, and functional foods. In this invention, the microcrystalline cellulose mainly serves as a main filler, increasing the total volume of the contents to ensure that each capsule, tablet, or unit dose meets the specifications suitable for production and processing. Simultaneously, microcrystalline cellulose has strong water absorption and swelling properties, rapidly absorbing water and swelling in the digestive tract, causing capsule shells to rupture or tablets to disintegrate, quickly dispersing the contents into fine particles, increasing the contact area between the active ingredients and the gastrointestinal mucosa, thereby promoting dissolution and absorption; its porous structure can also adsorb active ingredients, forming a uniform suspension upon disintegration, preventing the active ingredients from agglomerating and precipitating. Furthermore, the rough surface and regular shape of the microcrystalline cellulose particles improve the overall flowability of the powder, facilitating smooth mixing in the hopper, capsule filling, or tableting processes. It also has antistatic properties, reducing powder loss caused by electrostatic adsorption onto the inner walls of equipment, ensuring production yield. Microcrystalline cellulose is chemically stable and does not react chemically with the active ingredients in the anti-aging combination of this invention, thus not affecting the health benefits of the product. It is also suitable for various dosage forms such as capsules, tablets, and granules, providing a basis for the diversification of dosage forms of the composition of this invention.

[0025] In this invention, the lactose can be 10 parts, 20 parts, 30 parts, or 40 parts. The lactose primarily serves as a water-soluble filler to compensate for the insufficient water solubility of microcrystalline cellulose. Lactose dissolves rapidly in water, helping the contents to quickly wet and disperse in gastrointestinal fluids, thus increasing the dissolution rate of the active ingredients. When used in combination with microcrystalline cellulose in tablets, it can adjust the tablet's hardness and disintegration time, preventing excessively hard tablets from causing slow disintegration. Simultaneously, lactose has a natural sweetness that can mask some of the bitterness or off-flavors that may exist in the active ingredients, improving the palatability of the product, especially suitable for oral liquids, chewable tablets, and other dosage forms. In pharmaceutical manufacturing, an appropriate amount of lactose can improve powder flowability, reduce friction between particles, and facilitate uniform mixing and precise dispensing; it also has a certain degree of hygroscopicity, and adding an appropriate amount can adjust powder humidity, preventing static electricity caused by excessive drying, and preventing clumping during storage. Lactose has mild chemical properties and does not affect the long-term stability of the composition; when used in combination with microcrystalline cellulose, it can impart suitable hardness to the contents while maintaining disintegration properties. In addition, lactose is derived from cow's milk, is a natural excipient, has high safety, and is a recognized safe pharmaceutical excipient and food additive, meeting the raw material requirements for health products.

[0026] In this invention, microcrystalline cellulose and lactose are used in the above-mentioned proportions to form a "skeleton-water-soluble" biphase filling system. Microcrystalline cellulose provides a rigid skeleton to support disintegration, while lactose provides water-soluble channels to accelerate wetting. The synergy between the two gives the tablet / capsule contents both good formability and rapid release characteristics, providing a formulation guarantee for the timely absorption of active ingredients such as NMN, ergothioneine, coenzyme Q10, and PQQ in the composition.

[0027] In this invention, the magnesium stearate can be 8 parts, 12 parts, 15 parts, or 18 parts. The magnesium stearate primarily functions as a lubricant and flow aid. It forms an extremely thin hydrophobic lubricating film on the surface of the powder particles, significantly reducing friction between particles and between particles and equipment. This ensures smooth material flow and accurate dosage during capsule filling or tableting, reduces mechanical wear, extends equipment life, and prevents powder bridging or clogging in the hopper, ensuring continuous and stable production. In the tableting process, magnesium stearate prevents material from adhering to the punch and die surfaces, ensuring a smooth tablet surface and acceptable appearance. In capsule filling, it prevents powder from adhering to the filling rod and the inner wall of the capsule shell, reducing losses during filling. Furthermore, the hydrophobic film of magnesium stearate blocks moisture intrusion, reducing the risk of degradation of active ingredients due to moisture absorption, helping to extend the product's shelf life. It also prevents powder from clumping due to moisture or pressure during storage, maintaining good dispensing performance. Magnesium stearate is an internationally recognized safe pharmaceutical excipient, widely used in pharmaceuticals, health foods, and ordinary foods. It has good safety with long-term use and no risk of cumulative toxicity.

[0028] In this invention, the dosage form of the anti-aging composition is preferably at least one of tablets, capsules, and oral liquids. The preparation methods of the capsules, tablets, and oral liquids all use the anti-aging composition of this invention as the active ingredient, and produce the corresponding dosage forms through different formulation processes. Each dosage form can select a suitable combination of excipients according to actual production needs. For example, capsules and tablets use a combination of microcrystalline cellulose, lactose, and magnesium stearate, while oral liquids use microcrystalline cellulose and lactose (without the need for magnesium stearate), and suitable flavoring agents can be added according to taste requirements.

[0029] This invention provides a method for preparing the anti-aging composition, comprising the following steps: mixing β-nicotinamide mononucleotide, disodium pyrroloquinoline quinone, ergothioneine, coenzyme Q10, and L-selenomethylselenocysteine ​​according to the weight parts of the components, and obtaining a mixed powder as the anti-aging composition. Before mixing, it is preferable to pass each component through an 80-mesh sieve and collect the sieve residue for later use. The method for achieving uniform mixing is preferably to use a hopper mixer. Each component is added to the hopper mixer and mixed at a speed of 10-20 r / min for 20-40 minutes until uniform, thus obtaining the mixed powder. Since the amount of L-selenomethylselenocysteine ​​used is relatively low, it is preferable to first premix L-selenomethylselenocysteine ​​with the excipients using an equal-volume incremental method to obtain a premix, and then add the premix and the remaining active ingredients to the hopper mixer for final mixing.

[0030] In this invention, when the anti-aging composition is in capsule form, the outer shell of the capsule is preferably a #1 capsule shell. The capsule preparation method preferably includes the following steps: filling the mixed powder into a #1 capsule shell using a capsule filling machine. The volume of the #1 capsule shell is approximately 0.50 ml, which is sufficient to hold an effective dose of contents while facilitating swallowing. The ambient temperature during the filling process is preferably controlled at 18-26°C, but can be 21-25°C, or even 22°C, 23°C, or 24°C. The relative humidity is preferably no more than 45%.

[0031] In this invention, the mixed powder can also be mixed with excipients to form granules, tablets, or oral liquids. When the dosage form of the anti-aging composition is tablets, it is preferable to granulate the mixed powder using wet granulation or dry granulation, and then compress it into tablets to obtain tablets. For wet granulation, it is preferable to mix β-nicotinamide mononucleotide, disodium pyrrolidone, ergothioneine, coenzyme Q10, and L-selenomethylselenocysteine ​​with microcrystalline cellulose and lactose, add an appropriate amount of binder to form a soft mass, sieve and granulate, dry and granulate, and then add magnesium stearate and mix well. For dry granulation, the above mixed powder is granulated using a dry granulation device, and then magnesium stearate is added and mixed well to obtain total mixed granules. The total mixed granules are then placed in a tablet press and compressed into tablets to obtain tablets. When the dosage form of the anti-aging composition is oral liquid, it is preferable to dissolve each active ingredient in purified water, add flavoring agents, preservatives, and other excipients, filter, and then fill into oral liquid bottles to obtain the final product.

[0032] This invention provides an application of the aforementioned anti-aging composition in the preparation of anti-aging products.

[0033] In this invention, aging preferably includes skin aging and / or decline in bodily functions. Skin aging refers to the degenerative changes in structure and function of the skin that occur with age or due to internal and external factors such as oxidative stress and ultraviolet radiation. Specifically, it manifests as decreased skin luster, dull skin tone, reduced skin elasticity, increased fine lines and wrinkles, increased skin roughness, and decreased skin hydration. The anti-aging composition provided by this invention, through the synergistic effect of five active ingredients, can enhance NAD5 levels. + This product works by simultaneously promoting cellular energy metabolism, scavenging excess free radicals to reduce oxidative damage, and promoting mitochondrial regeneration and repair, thus delaying skin aging. The synergistic effect of its components is significantly superior to that of a single component or a simple combination of components. The aforementioned decline in bodily function refers to the gradual decrease in the physiological functions of various organ systems as we age, specifically manifested as decreased physical strength, lack of energy, fatigue, and reduced daily activity capacity.

[0034] In this invention, the anti-aging effect preferably includes improving facial skin radiance and / or alleviating physical fatigue associated with aging. Improving facial skin radiance refers to eliminating excess free radicals in skin cells, reducing oxidative damage, and promoting skin cell energy metabolism, resulting in a brighter, more translucent complexion and reduced dullness, which, from the user's subjective perspective, manifests as improved skin condition and a better complexion. Alleviating physical fatigue associated with aging refers to increasing the body's NAD+ levels. + It improves the level of mitochondrial function, optimizes energy metabolism, and effectively alleviates common subjective discomforts in middle-aged and elderly people, such as physical weakness, low spirits, and fatigue, making users more energetic and significantly improving their physical and mental state in daily work and life.

[0035] In this embodiment of the invention, after 28 days of oral administration of the anti-aging composition provided by this invention, 90% of the subjects scored ≥80 points in facial skin radiance, significantly higher than the 70% in the control group (anti-aging composition without L-seleno-methylselenocysteine); 85% of the subjects scored ≥80 points in body feedback, significantly higher than the 65% in the control group. This indicates that the composition of this invention is superior to the anti-aging composition lacking L-seleno-methylselenocysteine ​​in improving skin radiance and relieving physical fatigue, fully demonstrating the synergistic effect of the five components used in combination.

[0036] Unless otherwise specified, the materials used in this invention are commercially available products in the field.

[0037] To further illustrate the present invention, the solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0038] The sources of the above-mentioned raw materials in this invention are shown in Table 1.

[0039] Table 1. Sources of raw materials

[0040] Example 1 A method for preparing an anti-aging composition includes the following steps: Weigh out 1000g of NMN, 10g of PQQ, 100g of ergothioneine, 50g of coenzyme Q10 and 15mg of L-seleno-methylselenocysteine, mix them evenly using a hopper mixer, and then fill them into No. 1 capsule shells.

[0041] Example 2 A method for preparing an anti-aging composition, comprising the following steps, by weight: Weigh out 200g of NMN, 100g of PQQ, 10g of ergothioneine, 100g of coenzyme Q10 and 30mg of L-seleno-methylselenocysteine, mix them evenly using a hopper mixer, and then fill them into No. 1 capsule shells.

[0042] Example 3 A method for preparing an anti-aging composition, comprising the following steps, by weight: Weigh out 300g of NMN, 20g of PQQ, 30g of ergothioneine, 25g of coenzyme Q10 and 50mg of L-seleno-methylselenocysteine, mix them evenly using a hopper mixer, and then fill them into No. 1 capsule shells.

[0043] Example 4 A method for preparing an anti-aging composition, comprising the following steps, by weight: Weigh out 500g of NMN, 250g of PQQ, 1000g of ergothioneine, 750g of coenzyme Q10 and 100mg of L-seleno-methylselenocysteine, mix them evenly using a hopper mixer, and then fill them into No. 1 capsule shells.

[0044] Example 5 A method for preparing an anti-aging composition, comprising the following steps, by weight: Weigh out 1g of NMN, 500g of PQQ, 2000g of ergothioneine, 1500g of coenzyme Q10, and 200mg of L-seleno-methylselenocysteine. Mix them evenly using a hopper mixer and then fill them into No. 1 capsule shells.

[0045] Example 6 Mixing uniformity test Comparatively, the mixtures prepared in Examples 1-5 were analyzed at three points to assess their mixing homogeneity using HPLC. The PQQ column was a C18 column, eluted with methanol-water at a detection wavelength of 365.8 nm; the NMN column was a C18 column, eluted with phosphate buffer / phosphate solution-acetonitrile system at a detection wavelength of 260 nm; the ergothioneine column was a HILIC column, eluted with acetonitrile-ammonium acetate / formic acid aqueous solution system at a detection wavelength of 254 nm; the coenzyme Q10 column was a C18 column, eluted with anhydrous ethanol-water at a detection wavelength of 275 nm; and the L-seleno-methylselenocysteine ​​column was a chiral column, eluted with copper sulfate aqueous solution at a detection wavelength of 240 nm. The results for each component are shown in Table 2. The results indicate that the RSD values ​​of all components in Examples 1-5 of this invention are ≤1.6%, indicating good mixing homogeneity.

[0046] Table 2 HPLC detection results of Examples 1-5

[0047] Comparative Example 1 A method for preparing an anti-aging composition, comprising the following steps, by weight: Weigh 20g of PQQ and fill it into capsule shell #1.

[0048] Comparative Example 2 A method for preparing an anti-aging composition, comprising the following steps, by weight: Weigh 20g of PQQ and 300g of NMN, mix them evenly using a hopper mixer, and fill them into #1 capsule shells.

[0049] Comparative Example 3 A method for preparing an anti-aging composition, comprising the following steps, by weight: Weigh out 20g of PQQ, 300g of NMN, 30g of ergothioneine, and 25g of coenzyme Q10, mix them evenly using a hopper mixer, and fill them into #1 capsule shells.

[0050] Comparative Example 4 A method for preparing an anti-aging composition, comprising the following steps, by weight: Weigh out 20g of PQQ, 25g of ginsenosides, 30g of ergothioneine, 50g of GABA and 100mg of L-seleno-methylselenocysteine, mix them evenly in a hopper mixer, and then fill them into No. 1 capsule shells.

[0051] Comparative Example 5 A method for preparing an anti-aging composition, comprising the following steps, by weight: Weigh out 300g of PQQ, 20g of NMN, 25g of ginsenosides and 25g of coenzyme Q10, mix them evenly using a hopper mixer, and then fill them into #1 capsule shells.

[0052] Example 7 Accelerated stability assessment The capsules prepared in Examples 1-5 and Comparative Examples 1-5 were placed in high-density polyethylene bottles and stored under accelerated conditions (40℃±2℃, 75%RH±5%RH) for 3 months. The contents of PQQ, NMN, ergothioneine, coenzyme Q10 and L-seleno-methylselenocysteine ​​were then detected by HPLC. The results are shown in Tables 3 and 4.

[0053] As shown in Tables 3 and 4, the content of each component in the embodiments of the present invention was ≥98.6% after being placed under accelerated conditions for 3 months, which was higher than the content of the corresponding components in the comparative example. This indicates that the multi-component compound composition has better formulation stability than single or partial component combinations.

[0054] Table 3. HPLC detection results of Examples 1-5 after 3 months of acceleration

[0055] Table 4. HPLC detection results of Comparative Examples 1-5 after 3 months of accelerated processing.

[0056] Example 8 Human trial evaluation Using facial skin radiance and body feedback as the core evaluation dimensions, 100 male and 100 female subjects were randomly selected, aged 35-50 years and weighing 50-80 kg. All participants had to meet at least two of the following signs of aging: significantly decreased facial skin radiance, reduced elasticity, increased fine lines / wrinkles, and fatigue or decreased physical strength. Exclusion criteria included: those who had taken similar anti-aging or antioxidant supplements within the past 3 months; those with severe cardiovascular, cerebrovascular, liver, kidney, or metabolic diseases; pregnant or breastfeeding women; and those allergic to any component of this invention.

[0057] Two hundred participants were numbered sequentially from 1# to 200#, and the Visual Analogue Scale (VAS) was used to evaluate facial skin radiance and sensory feedback. The core principle of the VAS method is to transform the subjective, complex, and difficult-to-quantify experiences (such as taste) of the participants into numerical values ​​that are easy to statistically analyze, using a 10-centimeter-long visual line segment. In this study, VAS scores were divided into six levels: Very Poor (0 points), Poor (20 points), Neutral (40 points), Good (60 points), Excellent (80 points), and Very Good (100 points). Participants marked corresponding positions on the line segment based on their own feelings. Evaluators measured the distance of the marked point from the left end and converted it into a score from 0 to 100, with a score ≥80 defined as "significant improvement." The evaluation criteria are shown in Tables 5 and 6.

[0058] Table 5 Facial Skin Glow (Visual Dimension)

[0059] Table 6. Sensory Feedback (Dimensions of Subjective Feelings Throughout the Body)

[0060] Subjects numbered 1#~50# (males) and 101#~150# (females) took one capsule twice daily for 28 days, following the sample from Example 3. Subjects numbered 51#~100# (males) and 151#~200# (females) took one capsule twice daily for 28 days, following the sample from Comparative Example 3. Skin radiance and overall comfort were observed after 14 and 28 days of oral administration. A questionnaire was administered to assess product satisfaction. Results are shown below. Figure 1 .

[0061] The results showed that after 28 days of oral administration, 90% of the subjects in Example 3 scored ≥80 points in facial skin radiance, significantly higher than 70% in Comparative Example 3; and 85% of the subjects scored ≥80 points in body feedback, significantly higher than 65% in Comparative Example 3. This indicates that the composition of the present invention (Example 3) is superior to the composition lacking L-seleno-methylselenocysteine ​​(Comparative Example 3) in improving skin radiance and relieving physical fatigue, fully demonstrating the synergistic effect of the five components used in combination.

[0062] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. An anti-aging composition, characterized in that, It includes the following components by weight: 1-500 parts of disodium pyrroloquinoline quinone, 20-1000 parts of β-nicotinamide mononucleotide, 10-2000 parts of ergothioneine, 10-1500 parts of coenzyme Q10 and 0.015-0.6 parts of L-seleno-methylselenocysteine.

2. The anti-aging composition according to claim 1, characterized in that, It includes the following components by weight: 10-30 parts of disodium pyrroloquinoline quinone, 200-400 parts of β-nicotinamide mononucleotide, 15-45 parts of ergothioneine, 10-40 parts of coenzyme Q10 and 0.03-0.07 parts of L-seleno-methylselenocysteine.

3. The anti-aging composition according to claim 1 or 2, characterized in that, The anti-aging composition also includes excipients; The excipients include at least one of the following components: 20-50 parts microcrystalline cellulose, 5-50 parts lactose, and 5-20 parts magnesium stearate.

4. The anti-aging composition according to any one of claims 1 to 3, characterized in that, The dosage form of the anti-aging composition is at least one of tablets, capsules, granules, and oral liquids.

5. A method for preparing the anti-aging composition according to any one of claims 1 to 4, characterized in that, Includes the following steps: The mixed powder obtained by mixing β-nicotinamide mononucleotide, disodium pyrroloquinoline quinone, ergothioneine, coenzyme Q10 and L-seleno-methylselenocysteine ​​according to the weight parts of the components is an anti-aging composition.

6. The preparation method according to claim 5, characterized in that, When the mixed powder is filled into capsule shells, the dosage form is capsules.

7. The preparation method according to claim 5, characterized in that, The mixed powder and excipients are then mixed to form granules, tablets, or oral liquids.

8. The use of the anti-aging composition according to any one of claims 1 to 4 in the preparation of anti-aging products.

9. The application according to claim 8, characterized in that, The aging process includes skin aging and / or decline in bodily functions.

10. The application according to claim 8 or 9, characterized in that, The anti-aging benefits include improving facial skin radiance and / or relieving physical fatigue associated with aging.

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