Preparation of a mixed hair growth agent of traditional Chinese medicine exosome and hair papilla cell exosome

Abstract

The application provides a preparation of a mixed hair growth agent of traditional Chinese medicine exosomes and dermal papilla cell exosomes, and relates to the technical field of hair loss prevention and treatment. The mixed hair growth agent of traditional Chinese medicine exosomes and dermal papilla cell exosomes is mainly prepared from radix falcaliae, ligustrum lucidum, ginger, dendrobium, winter mulberry leaves and wormwood by the method of mixing traditional Chinese medicine exosomes and dermal papilla cell exosomes, and then mixed with dipalmitoyl phosphatidylcholine and isopropyl palmitate for high-speed shearing homogenization, and then mixed with carbomer, sodium thiosulfate and sodium carboxymethyl cellulose for high-speed stirring to obtain the mixed hair growth agent. The application overcomes the shortcomings of the prior art, can effectively reduce hair loss, effectively promote hair growth of hair follicles, achieve a good anti-hair-loss effect, reduce the use amount of dermal papilla cell exosomes, reduce production cost, and is convenient for commercial production and preparation.

Description

Technical Field

[0001] This invention relates to the field of hair loss prevention and treatment technology, specifically to the preparation of a hair growth agent that combines traditional Chinese medicine exosomes with dermal papilla cell exosomes. Background Technology

[0002] Hair loss is a prevalent skin condition worldwide. Up to 40% of women and 50% of men have experienced thinning hair. Many factors can contribute to hair loss, including increased sensitivity of hair follicles to dihydrotestosterone (DHT), autoimmune reactions, hormonal imbalances, genetic factors, environmental factors, medications, nutritional deficiencies, and stress.

[0003] Currently, the most effective treatments for hair loss are finasteride and minoxidil. However, their efficacy is controversial, and they have significant side effects. Finasteride is an oral medication, and taking it can cause sexual dysfunction. Minoxidil is a topical application, but short-term use can lead to significant hair loss, and its effectiveness is poor in some individuals.

[0004] Exosomes are small membrane vesicles containing complex RNA and proteins. Existing research has shown that human umbilical cord mesenchymal stem cell exosomes and dermal papilla cell exosomes can promote hair follicle development to a certain extent. However, these types of exosomes are difficult to obtain, while traditional Chinese medicine ingredients are relatively easy to obtain. Whether the preparation of exosomes using traditional Chinese medicine combined with dermal papilla cell exosomes can further improve the treatment of hair loss is a major research direction at present. Summary of the Invention

[0005] To address the shortcomings of existing technologies, this invention provides a method for preparing a hair growth agent that combines traditional Chinese medicine exosomes with dermal papilla cell exosomes. This agent can effectively reduce hair loss and promote hair follicle growth, achieving a good anti-hair loss effect. At the same time, it reduces the amount of dermal papilla cell exosomes required, lowers production costs, and facilitates commercial production.

[0006] To achieve the above objectives, the technical solution of the present invention is implemented through the following technical solution:

[0007] Preparation of a hair growth agent consisting of a mixture of traditional Chinese medicine exosomes and dermal papilla cell exosomes, comprising the following steps:

[0008] S1. Preparation of exosome A from traditional Chinese medicine: Polygonum multiflorum and Ligustrum lucidum were mixed and extracted by ultrasonic extraction with water as solvent, followed by centrifugation to obtain exosome A from traditional Chinese medicine.

[0009] S2. Preparation of exosome B from traditional Chinese medicine: ginger, dendrobium, mulberry leaf and artemisia were selected and extracted by water bath and freeze grinding with water as solvent, and then centrifuged to obtain exosome B from traditional Chinese medicine.

[0010] S3. Raw material premixing: Mix Chinese herbal exosomes A, Chinese herbal exosomes B and dermal papilla cell exosomes, add 2 times the volume of ethanol solution, add dipalmitoylphosphatidylcholine, then remove ethanol by rotary evaporation, add isopropyl palmitate, deionized water and surfactant, perform high-speed shearing and homogenization to obtain a composite emulsion for later use.

[0011] S4. Mixing of excipients: Dissolve carbomer, sodium thiosulfate and sodium carboxymethyl cellulose in deionized water and stir evenly in a water bath to obtain an excipient solution for later use.

[0012] S5. Preparation of mixed hair growth agent: Mix the compound emulsion and the excipient solution, stir at high speed, and let stand to remove bubbles to obtain the mixed hair growth agent.

[0013] Preferably, the mass ratio of Polygonum multiflorum and Ligustrum lucidum in step S1 is 2:1.

[0014] Preferably, the preparation of the traditional Chinese medicine exosome A in step S1 specifically includes the following steps:

[0015] S1-1. Mix Polygonum multiflorum and Ligustrum lucidum, add 2 times the volume of deionized water and grind into a slurry. Then, sonicate at 600W for 15-20 minutes and filter to obtain the filtrate.

[0016] S1-2. The above filtrate is centrifuged sequentially at centrifugal forces of 2000-6000g, 10000-15000g, and 80000-120000g for 80-100min respectively. The final precipitate is resuspended in deionized water and the lower layer is taken to obtain the Chinese medicine exosome A.

[0017] Preferably, the mass ratio of ginger, dendrobium, mulberry leaves and mugwort in step S2 is 1:1:2:2.

[0018] Preferably, the preparation of the traditional Chinese medicine exosome B in step S2 specifically includes the following steps:

[0019] S2-1. Mix ginger, dendrobium, mulberry leaves and mugwort, add 2 times the amount of deionized water and grind into a slurry. Keep warm in a water bath at 45-55℃ for 1-2 hours, then freeze at -13℃--15℃ for 4-6 hours. Continue grinding into a slurry and then filter to obtain the filtrate for later use.

[0020] S2-2. Centrifuge the filtrate sequentially at 1200g, 6000g, and 12000g for 20-30 minutes to obtain the supernatant. Centrifuge the supernatant at 120000g for 80 minutes. Take the precipitate, resuspend it in purified water, and then take the lower layer to obtain the Chinese medicine exosome B.

[0021] Preferably, the mass fractions of each raw material in step S3 are as follows: 8-10 parts of Chinese herbal exosome A, 4-5 parts of Chinese herbal exosome B, 1-2 parts of dermal papilla cell exosomes, 0.2-0.6 parts of dipalmitoylphosphatidylcholine, 4-10 parts of isopropyl palmitate, 2-4 parts of surfactant, and 15-30 parts of deionized water.

[0022] Preferably, the high-speed shearing and homogenization in step S3 are performed by using a high-speed shearing machine, wherein the shearing speed is 10,000-20,000 rpm, the shearing time is 60-80 s, the homogenization pressure is 18-30 MPa, and the homogenization time is 10-15 min.

[0023] Preferably, the mass fractions of each raw material in step S4 are as follows: 2-4 parts carbomer, 1-3 parts sodium thiosulfate, 1-3 parts sodium carboxymethyl cellulose, and 15-20 parts deionized water.

[0024] Preferably, the temperature of the water bath stirring in step S4 is 40-50℃.

[0025] Preferably, in step S5, the composite emulsion and the excipient solution are mixed in equal proportions, and the high-speed stirring speed is 1000-1200 r / min, and the stirring time is 20-30 min.

[0026] This invention provides a method for preparing a hair growth agent that combines traditional Chinese medicine exosomes with dermal papilla cell exosomes. Compared with existing technologies, this method has the following advantages:

[0027] This invention utilizes the combined action of traditional Chinese medicine exosomes and dermal papilla cell exosomes to effectively promote hair growth in hair follicles and shorten the hair growth cycle. Simultaneously, a composite emulsion is prepared by high-speed shearing of isopropyl palmitate and deionized water, which is applied topically. This effectively promotes the absorption of exosomes while reducing treatment pain and improving the convenience of treatment. The overall ingredients have low toxicity and side effects while ensuring the prevention and treatment of hair loss. Detailed Implementation

[0028] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0029] Example 1:

[0030] Preparation of exosomes from traditional Chinese medicine:

[0031] 1. Preparation of exosome A from traditional Chinese medicine:

[0032] (1) Mix Polygonum multiflorum and Ligustrum lucidum in a mass ratio of 2:1, add 2 times the volume of deionized water and grind into a slurry, then sonicate at 600W for 18 minutes, and then filter to obtain the filtrate.

[0033] (2) The above filtrate was centrifuged for 80 minutes at centrifugal forces of 4000g, 15000g and 120000g respectively. The final precipitate was resuspended in deionized water and the lower layer was taken to obtain the Chinese medicine exosome A.

[0034] 2. Preparation of exosomes B from traditional Chinese medicine:

[0035] (1) Mix ginger, dendrobium, mulberry leaves and mugwort in a mass ratio of 1:1:2:2, add 2 times the amount of deionized water and grind into a slurry. Keep warm in a 50°C water bath for 1.5 hours, freeze at -13°C for 5 hours, continue grinding into a slurry and then filter to obtain the filtrate for later use.

[0036] (2) The filtrate was centrifuged at 1200g, 6000g and 12000g for 25min in sequence to obtain the supernatant; the supernatant was centrifuged at 120000g for 80min, the precipitate was resuspended in purified water and the lower layer was taken to obtain the Chinese medicine exosome B.

[0037] 3. Preparation of exosomes C from traditional Chinese medicine:

[0038] (1) Mix Polygonum multiflorum, Ligustrum lucidum, ginger, Dendrobium nobile, mulberry leaf and Artemisia argyi in a mass ratio of 2:1:0.5:0.5:1:1, add 2 times the volume of deionized water and grind into a paste, then sonicate at 600W for 18 minutes, and then filter to obtain the filtrate.

[0039] (2) The above filtrate was centrifuged for 80 minutes at centrifugal forces of 4000g, 15000g and 120000g respectively. The final precipitate was resuspended in deionized water and the lower layer was taken to obtain the Chinese medicine exosome A.

[0040] 4. Preparation of exosomes D from traditional Chinese medicine:

[0041] (1) Mix Polygonum multiflorum, Ligustrum lucidum, ginger, Dendrobium nobile, mulberry leaf and Artemisia argyi in a mass ratio of 2:1:0.5:0.5:1:1, add 2 times the amount of deionized water and grind into a slurry. Keep warm in a water bath at 50℃ for 1.5h, freeze at -13℃ for 5h, continue grinding into a slurry and then filter to obtain the filtrate for later use.

[0042] (2) The filtrate was centrifuged at 1200g, 6000g and 12000g for 25min in sequence to obtain the supernatant; the supernatant was centrifuged at 120000g for 80min, the precipitate was resuspended in purified water and the lower layer was taken to obtain the Chinese medicine exosome D.

[0043] Example 2:

[0044] Preparation of hair growth products:

[0045] (1) Material preparation:

[0046] The main ingredients are prepared according to the following weight proportions: 9 parts of Chinese herbal exosome A, 4.5 parts of Chinese herbal exosome B, 1.5 parts of dermal papilla cell exosomes, 0.4 parts of dipalmitoylphosphatidylcholine, 6 parts of isopropyl palmitate, 3 parts of sodium dodecylbenzenesulfonate, and 15-30 parts of deionized water.

[0047] The excipients are prepared according to the following parts by weight: 3 parts carbomer, 2 parts sodium thiosulfate, 2 parts sodium carboxymethyl cellulose, and 18 parts deionized water.

[0048] (2) Mix Chinese herbal exosomes A, Chinese herbal exosomes B and dermal papilla cell exosomes, add 2 times the volume of ethanol solution, add dipalmitoylphosphatidylcholine, remove ethanol by rotary evaporation, add isopropyl palmitate, deionized water and sodium dodecylbenzene sulfonate, and shear using a high-speed shearing machine with a shearing speed of 150,000 rpm and a shearing time of 70 s. The homogenization pressure is 22 MPa and the homogenization time is 12 min to obtain a composite emulsion.

[0049] (3) Mix carbomer, sodium thiosulfate and sodium carboxymethyl cellulose in deionized water and stir evenly in a water bath at 45°C to obtain an excipient solution for later use.

[0050] (4) Mix the composite emulsion and the excipient solution in equal proportions, stir at 1100r / min for 25min, and then let stand to remove bubbles to obtain the mixed hair growth agent.

[0051] Comparative Example 1:

[0052] Preparation of hair growth products:

[0053] (1) Material preparation:

[0054] The main ingredients are prepared according to the following weight proportions: 3.5 parts of Chinese herbal exosomes C1, 1.5 parts of dermal papilla cell exosomes, 0.4 parts of dipalmitoylphosphatidylcholine, 6 parts of isopropyl palmitate, 3 parts of sodium dodecylbenzenesulfonate, and 15-30 parts of deionized water.

[0055] The excipients are prepared according to the following parts by weight: 3 parts carbomer, 2 parts sodium thiosulfate, 2 parts sodium carboxymethyl cellulose, and 18 parts deionized water.

[0056] (2) Mix the Chinese herbal exosome C and dermal papilla cell exosomes, add 2 times the volume of ethanol solution, add dipalmitoylphosphatidylcholine, remove the ethanol by rotary evaporation, add isopropyl palmitate, deionized water and sodium dodecylbenzene sulfonate, and shear using a high-speed shearing machine with a shearing speed of 150,000 rpm and a shearing time of 70 s. The homogenization pressure is 22 MPa and the homogenization time is 12 min to obtain a composite emulsion.

[0057] (3) Mix carbomer, sodium thiosulfate and sodium carboxymethyl cellulose in deionized water and stir evenly in a water bath at 45°C to obtain an excipient solution for later use.

[0058] (4) Mix the composite emulsion and the excipient solution in equal proportions, stir at 1100r / min for 25min, and then let stand to remove bubbles to obtain the hair growth agent.

[0059] Comparative Example 2:

[0060] Preparation of hair growth products:

[0061] (1) Material preparation:

[0062] The main ingredients are prepared according to the following weight proportions: 3.5 parts of Chinese herbal exosome D1, 1.5 parts of dermal papilla cell exosomes, 0.4 parts of dipalmitoylphosphatidylcholine, 6 parts of isopropyl palmitate, 3 parts of sodium dodecylbenzenesulfonate, and 15-30 parts of deionized water.

[0063] The excipients are prepared according to the following parts by weight: 3 parts carbomer, 2 parts sodium thiosulfate, 2 parts sodium carboxymethyl cellulose, and 18 parts deionized water.

[0064] (2) Mix the Chinese herbal exosomes D and dermal papilla cell exosomes, add 2 times the volume of ethanol solution, add dipalmitoylphosphatidylcholine, remove the ethanol by rotary evaporation, add isopropyl palmitate, deionized water and sodium dodecylbenzene sulfonate, and shear using a high-speed shearing machine with a shearing speed of 150,000 rpm and a shearing time of 70 s. The homogenization pressure is 22 MPa and the homogenization time is 12 min to obtain a composite emulsion.

[0065] (3) Mix carbomer, sodium thiosulfate and sodium carboxymethyl cellulose in deionized water and stir evenly in a water bath at 45°C to obtain an excipient solution for later use.

[0066] (4) Mix the composite emulsion and the excipient solution in equal proportions, stir at 1100r / min for 25min, and then let stand to remove bubbles to obtain the hair growth agent.

[0067] Comparative Example 3:

[0068] Preparation of hair growth products:

[0069] (1) Material preparation:

[0070] The main ingredients are prepared according to the following weight proportions: 9 parts of Chinese herbal exosome A, 6 parts of Chinese herbal exosome B, 0.4 parts of dipalmitoylphosphatidylcholine, 6 parts of isopropyl palmitate, 3 parts of sodium dodecylbenzenesulfonate, and 15-30 parts of deionized water.

[0071] The excipients are prepared according to the following parts by weight: 3 parts carbomer, 2 parts sodium thiosulfate, 2 parts sodium carboxymethyl cellulose, and 18 parts deionized water.

[0072] (2) Mix Chinese herbal exosomes A and B and add 2 times the volume of ethanol solution, then add dipalmitoylphosphatidylcholine, then remove the ethanol by rotary evaporation, then add isopropyl palmitate, deionized water and sodium dodecylbenzenesulfonate, and shear using a high-speed shearing machine, where the shearing speed is 150,000 rpm, the shearing time is 70 s, the homogenization pressure is 22 MPa, and the homogenization time is 12 min to obtain a composite emulsion;

[0073] (3) Mix carbomer, sodium thiosulfate and sodium carboxymethyl cellulose in deionized water and stir evenly in a water bath at 45°C to obtain an excipient solution for later use.

[0074] (4) Mix the composite emulsion and the excipient solution in equal proportions, stir at 1100r / min for 25min, and then let stand to remove bubbles to obtain the mixed hair growth agent.

[0075] Comparative Example 4:

[0076] (1) Material preparation:

[0077] The main ingredients are prepared according to the following weight proportions: 15 parts of dermal papilla cell exosomes, 0.4 parts of dipalmitoylphosphatidylcholine, 6 parts of isopropyl palmitate, 3 parts of sodium dodecylbenzenesulfonate, and 15-30 parts of deionized water.

[0078] The excipients are prepared according to the following parts by weight: 3 parts carbomer, 2 parts sodium thiosulfate, 2 parts sodium carboxymethyl cellulose, and 18 parts deionized water.

[0079] (2) Add 2 times the volume of ethanol solution to the exosomes of dermal papilla cells, then add dipalmitoylphosphatidylcholine, then remove the ethanol by rotary evaporation, then add isopropyl palmitate, deionized water and sodium dodecylbenzene sulfonate, and shear using a high-speed shearing machine, wherein the shearing speed is 150,000 rpm, the shearing time is 70 s, the homogenization pressure is 22 MPa, and the homogenization time is 12 min to obtain a composite emulsion.

[0080] (3) Mix carbomer, sodium thiosulfate and sodium carboxymethyl cellulose in deionized water and stir evenly in a water bath at 45°C to obtain an excipient solution for later use.

[0081] (4) Mix the composite emulsion and the excipient solution in equal proportions, stir at 1100r / min for 25min, and then let stand to remove bubbles to obtain the mixed hair growth agent.

[0082] Detection:

[0083] The control group was designed with physiological saline as control group 1 and 2% minoxidil solution as control group 2.

[0084] 1. Take 35 male and 35 female C57BL / 6 mice at 6 weeks of age and randomly divide them into 7 groups, with 5 male mice and 5 female mice in each group. Remove the hair on the back of the mice and control the hair removal area to be 4cm×4cm. Feed them normally.

[0085] On the day following hair removal, the hair growth agents prepared in Example 2 and Comparative Examples 1-4, physiological saline, and 2% minoxidil solution were applied to the backs of mice in each group at a rate of 3 ml / kg, twice daily (morning and evening) for 10 consecutive days. The mice were observed for redness, swelling, breakage, or other lesions on their backs during the application process. On day 10, skin samples from the hair-removed areas of the mice's backs were taken and prepared for pathological examination to observe hair follicle growth. The specific results are shown in Table 1 below.

[0086] Table 1

[0087]

[0088] 2. Take 14 male and 14 female C57BL / 6 mice at 6 weeks of age and randomly divide them into 7 groups, with 2 male mice and 2 female mice in each group. Remove the hair on the back of the mice and control the hair removal area to be 4cm×4cm. Feed them normally.

[0089] On the day following hair removal, the hair growth agents prepared in Example 2 and Comparative Examples 1-4, physiological saline, and 2% minoxidil solution were applied to the backs of mice in each group at a rate of 3 ml / kg, twice daily (morning and evening). On days 15, 18, and 21 after application, five hairs (with follicles and no breakage) were randomly plucked from the application site of the mice, and their lengths were measured. The average length was then calculated. The specific results are shown in Table 2 below.

[0090] Table 2

[0091]

[0092] As can be seen from the above tests, the hair growth agent prepared in Example 2 of this application can effectively promote hair follicle growth, and the hair growth effect of the hair growth agent can be effectively improved by using different solvents to extract exosomes from different Chinese medicines and then compounding them.

[0093] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. Preparation of a hair growth agent consisting of a mixture of traditional Chinese medicine exosomes and dermal papilla cell exosomes, characterized in that, The preparation of the mixed hair growth agent includes the following steps: S1. Preparation of exosome A from traditional Chinese medicine: Polygonum multiflorum and Ligustrum lucidum were mixed and extracted by ultrasonic extraction with water as solvent, followed by centrifugation to obtain exosome A from traditional Chinese medicine. S2. Preparation of exosome B from traditional Chinese medicine: ginger, dendrobium, mulberry leaf and artemisia were selected and extracted by water bath and freeze grinding with water as solvent, and then centrifuged to obtain exosome B from traditional Chinese medicine. S3. Raw material premixing: Mix Chinese herbal exosomes A, Chinese herbal exosomes B and dermal papilla cell exosomes, add 2 times the volume of ethanol solution, add dipalmitoylphosphatidylcholine, then remove ethanol by rotary evaporation, add isopropyl palmitate, deionized water and surfactant, perform high-speed shearing and homogenization to obtain a composite emulsion for later use. S4. Mixing of excipients: Dissolve carbomer, sodium thiosulfate and sodium carboxymethyl cellulose in deionized water and stir evenly in a water bath to obtain an excipient solution for later use. S5. Preparation of mixed hair growth agent: Mix the compound emulsion and the excipient solution, stir at high speed, and let stand to remove bubbles to obtain the mixed hair growth agent.

2. The preparation of a hair growth agent consisting of a mixture of traditional Chinese medicine exosomes and dermal papilla cell exosomes according to claim 1, characterized in that: In step S1, the mass ratio of Polygonum multiflorum and Ligustrum lucidum is 2:

1.

3. The preparation of a hair growth agent consisting of a mixture of traditional Chinese medicine exosomes and dermal papilla cell exosomes according to claim 1, characterized in that, The preparation of the traditional Chinese medicine exosome A in step S1 specifically includes the following steps: S1-1. Mix Polygonum multiflorum and Ligustrum lucidum, add 2 times the volume of deionized water and grind into a slurry. Then, sonicate at 600W for 15-20 minutes and filter to obtain the filtrate. S1-2. The above filtrate is centrifuged sequentially at centrifugal forces of 2000-6000g, 10000-15000g, and 80000-120000g for 80-100min respectively. The final precipitate is resuspended in deionized water and the lower layer is taken to obtain the Chinese medicine exosome A.

4. The preparation of a hair growth agent consisting of a mixture of traditional Chinese medicine exosomes and dermal papilla cell exosomes according to claim 1, characterized in that: In step S2, the mass ratio of ginger, dendrobium, mulberry leaves and mugwort is 1:1:2:

2.

5. The preparation of a hair growth agent consisting of a mixture of traditional Chinese medicine exosomes and dermal papilla cell exosomes according to claim 1, characterized in that, The preparation of the traditional Chinese medicine exosome B in step S2 specifically includes the following steps: S2-1. Mix ginger, dendrobium, mulberry leaves and mugwort, add 2 times the amount of deionized water and grind into a slurry. Keep warm in a water bath at 45-55℃ for 1-2 hours, then freeze at -13℃--15℃ for 4-6 hours. Continue grinding into a slurry and then filter to obtain the filtrate for later use. S2-2. Centrifuge the filtrate sequentially at 1200g, 6000g, and 12000g for 20-30 minutes to obtain the supernatant. Centrifuge the supernatant at 120000g for 80 minutes. Take the precipitate, resuspend it in purified water, and then take the lower layer to obtain the Chinese medicine exosome B.

6. The preparation of a hair growth agent consisting of a mixture of traditional Chinese medicine exosomes and dermal papilla cell exosomes according to claim 1, characterized in that, The mass fractions of each raw material in step S3 are as follows: 8-10 parts of Chinese herbal exosome A, 4-5 parts of Chinese herbal exosome B, 1-2 parts of dermal papilla cell exosomes, 0.2-0.6 parts of dipalmitoylphosphatidylcholine, 4-10 parts of isopropyl palmitate, 2-4 parts of surfactant, and 15-30 parts of deionized water.

7. The preparation of a hair growth agent consisting of a mixture of traditional Chinese medicine exosomes and dermal papilla cell exosomes according to claim 1, characterized in that, The high-speed shearing and homogenization in step S3 are performed as follows: a high-speed shearing machine is used for shearing, with a shearing speed of 10,000-20,000 rpm and a shearing time of 60-80 seconds. The homogenization pressure is 18-30 MPa and the homogenization time is 10-15 minutes.

8. The preparation of a hair growth agent consisting of a mixture of traditional Chinese medicine exosomes and dermal papilla cell exosomes according to claim 1, characterized in that, The mass fractions of each raw material in step S4 are as follows: 2-4 parts carbomer, 1-3 parts sodium thiosulfate, 1-3 parts sodium carboxymethyl cellulose, and 15-20 parts deionized water.

9. The preparation of a hair growth agent consisting of a mixture of traditional Chinese medicine exosomes and dermal papilla cell exosomes according to claim 1, characterized in that, The temperature of the water bath stirring in step S4 is 40-50℃.

10. The preparation of a hair growth agent consisting of a mixture of traditional Chinese medicine exosomes and dermal papilla cell exosomes according to claim 1, characterized in that, In step S5, the composite emulsion and the excipient solution are mixed in equal proportions, and the high-speed stirring speed is 1000-1200 r / min, and the stirring time is 20-30 min.

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