Apparatus and method for preparing chicken bile extract

Abstract

The application discloses a chicken gall paste preparation device and method, which comprises a tank body and a tank cover arranged on the top of the tank body, a heating jacket is arranged outside the tank body, a driving motor is arranged on the tank cover, an output end of the driving motor is connected with a stirring shaft which is inserted into the tank body, a wall scraping frame and helical blades are respectively connected to the stirring shaft, a discharge pipe is arranged at the bottom of the tank body, the discharge pipe is communicated with an inlet of a three-way valve, a first outlet of the three-way valve is communicated with an annular distributor arranged on the upper part of the inner side of the tank body through a circulating pump and a circulating pipe in sequence, and a second outlet of the three-way valve forms a discharge branch line; the circulating pump can convey the concentrated material at the bottom of the tank body to the annular distributor, so that the material forms a downward flowing liquid film along the heated inner wall, and the wall scraping frame and the helical blades are used for scraping the wall and axially turning, thereby reducing the residence time of high-viscosity chicken gall thick paste on the heating wall surface and reducing local overheating and wall sticking.

Description

Technical Field

[0001] This invention relates to the field of extraction of effective components from animal bile and preparation of raw materials for traditional Chinese medicine preparations, and particularly to an apparatus and method for preparing fresh chicken bile paste. Background Technology

[0002] Chicken fresh bile extract is an extract obtained from fresh chicken bile through processes such as filtration, concentration, ethanol reflux extraction, solid-liquid separation, and ethanol recovery. It can be used in pharmaceutical preparations such as pertussis tablets. Chenocholic acid is one of the important indicator components for evaluating the quality of chicken fresh bile extract.

[0003] The current process for preparing fresh chicken bile extract typically requires the separate use of concentration equipment, reflux extraction equipment, and solvent recovery equipment. The material needs to be repeatedly transferred between different devices, which not only prolongs the operation but also easily leads to chicken bile extract residue remaining on the inner walls of the equipment and in the conveying pipelines, increasing material loss and batch-to-batch quality variations.

[0004] Fresh chicken bile has a high initial water content, and its viscosity increases significantly after concentration. When using conventional stirring methods, highly viscous material near the heating wall of the tank tends to remain there for extended periods, leading to adhesion, film formation, and localized overheating. This not only affects heat transfer efficiency but may also adversely impact components in the bile, such as chenodeoxycholic acid.

[0005] Furthermore, some existing processes require drying the concentrated chicken bile extract into a dry paste before ethanol reflux extraction, followed by pulverization and sieving before adding ethanol. This process adds drying, pulverization, and transfer steps, prolongs the material's heating time, and increases energy consumption. A key technical problem to be solved is how to directly extract concentrated chicken bile extract with a certain moisture content using ethanol reflux without drying and pulverization, while ensuring the chenodeoxycholic acid content in the resulting fresh chicken bile extract. Summary of the Invention

[0006] The purpose of this invention is to solve the problems existing in the prior art, and it proposes a device and method for preparing chicken bile paste.

[0007] To achieve the above objectives, the invention adopts the following technical solution: A device for preparing fresh chicken bile paste includes a tank and a lid on top of the tank. A heating jacket is provided on the outside of the tank. A drive motor is mounted on the lid, and the output end of the drive motor is connected to a stirring shaft extending into the tank. A scraper and spiral blades are connected to the stirring shaft. A discharge pipe is located at the bottom of the tank and is connected to the inlet of a three-way valve. The first outlet of the three-way valve is connected to an annular distributor located on the upper side of the tank via a circulation pump and a circulation pipe. The second outlet of the three-way valve forms a discharge branch. The lid is connected to a condenser via a steam pipe. The condensate outlet of the condenser is connected to a collection tank, which is connected to a return pipe and a recovery pipe. The return pipe is connected to the annular distributor.

[0008] Preferably, the scraper includes a transverse connecting part connected to the stirring shaft and scraper parts disposed at both ends of the transverse connecting part. The scraper parts extend vertically along the inner wall of the tank and are in clearance fit or elastic contact with the inner wall of the tank. The spiral blades are located between the two scraper parts.

[0009] Preferably, the annular feeder is arranged around the circumference of the tank body, and the lower part of the annular feeder is provided with a plurality of feed holes facing the inner wall of the tank body, and the plurality of feed holes are distributed at intervals around the circumference of the annular feeder.

[0010] Preferably, the circulation pipe and the return pipe are respectively connected to independent liquid inlets on the annular distributor, and the circulation pipe and the return pipe are respectively equipped with one-way valves to prevent fluid backflow.

[0011] Preferably, the liquid collection tank is installed at a height higher than the annular distributor, and the return pipe is connected to the bottom of the liquid collection tank so that the condensed ethanol in the liquid collection tank enters the annular distributor under the action of gravity.

[0012] Preferably, the collection tank is provided with a vacuum interface for connection to a vacuum device, and the reflux pipe and the recovery pipe are respectively provided with control valves, and the recovery pipe is connected to a sealed ethanol receiving container.

[0013] A method for preparing fresh chicken bile extract using the apparatus according to any one of claims 1-6, characterized by comprising the following steps: S1. After filtering the fresh chicken bile, add it to the tank. Vacuum the tank through the vacuum interface and indirectly heat the fresh chicken bile through the heating jacket. Concentrate under vacuum at a material temperature not exceeding 70°C until the moisture content of the resulting concentrated paste does not exceed 42.24%. S2. Without drying and pulverizing the concentrated paste, add 10 mL of 95% ethanol per gram of dry solids according to the mass of the concentrated paste, and reflux extract under reduced pressure for 1.5 h at a material temperature not exceeding 70°C. After the first reflux extraction is completed, perform solid-liquid separation. S3. Add 95% ethanol to the residue obtained from the first solid-liquid separation at 10 mL per gram of the initial dry solids. Reflux extract under reduced pressure for 1.5 h at a material temperature not exceeding 70°C. After the second reflux extraction is completed, perform solid-liquid separation and combine the extracts obtained from the two solid-liquid separations. S4. Close the reflux tube and open the recovery tube. Recover the ethanol in the extract at a temperature not exceeding 60°C. Then, vacuum concentrate the extract after ethanol recovery into a thick paste to obtain fresh chicken bile paste.

[0014] Preferably, during the vacuum concentration process in step S1, the circulation pump is turned on, so that the material at the bottom of the tank passes through the discharge pipe, the three-way valve, the circulation pump and the circulation pipe in sequence into the annular distributor, and is distributed downward along the inner wall of the tank by the annular distributor.

[0015] Preferably, during the reduced pressure reflux extraction process in steps S2 and S3, the circulation pump is turned off, and the ethanol vapor is condensed sequentially through the steam pipe and condenser before entering the collection tank. It then returns to the concentrated paste along the inner wall of the tank through the reflux pipe and the annular distributor. The solid-liquid separation is performed by centrifugal separation.

[0016] Preferably, the chenodeoxycholic acid content of the fresh chicken bile extract obtained in step S4 is not less than 15.0%.

[0017] The invention has the following advantages compared to the prior art: 1. This invention uses a circulating pump to transport the concentrated material at the bottom of the tank to an annular distributor, so that the material forms a downward flowing liquid film along the heated inner wall. With the help of a scraper and spiral blades, the material is scraped and axially turned, which can reduce the residence time of the high-viscosity chicken bile paste on the heated wall surface and reduce local overheating and sticking.

[0018] 2. This invention connects a ring-shaped distributor to both a material circulation pipe and an ethanol reflux pipe. During the concentration stage, the ring-shaped distributor is used for circulating and distributing the material; during the reflux stage, it is used to evenly distribute the condensed ethanol and flush the inner wall of the tank, allowing the same structure to adapt to different processes, reducing the number of devices and material transfer losses.

[0019] 3. This invention, through the cooperation between the vacuum interface, condenser, reflux pipe and recovery pipe, can complete ethanol reflux extraction at a temperature not exceeding 70°C and recover ethanol at a temperature not exceeding 60°C. This helps to reduce the thermal impact of bile components such as chenodeoxycholic acid and improve the quality stability of chicken bile paste. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the overall structure of the chicken fresh bile paste preparation device of the present invention; Figure 2 This is a schematic diagram of the connection structure between the wall scraper, the spiral blades, and the stirring shaft of the present invention; Figure 3 This is a schematic diagram of the connection structure of the wall scraper, spiral blades and stirring shaft of the present invention; Figure 4 This is a schematic diagram of the connection structure of the tank body, annular distributor, steam pipe and return pipe of the present invention.

[0021] In the diagram: 1. Tank body; 2. Tank cover; 3. Heating jacket; 4. Drive motor; 5. Stirring shaft; 6. Scraper; 7. Spiral blades; 8. Discharge pipe; 9. Circulation pump; 10. Three-way valve; 11. Circulation pipe; 12. Annular distributor; 13. Steam pipe; 14. Condenser; 15. Collection tank; 16. Return pipe; 17. Recovery pipe; 18. Vacuum interface. Detailed Implementation

[0022] The technical solutions in the embodiments of the invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the invention, and not all embodiments.

[0023] In the description of the invention, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing the invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the invention.

[0024] Reference Figures 1-4 A device for preparing fresh chicken bile paste includes a tank body 1 and a tank lid 2. The tank body 1 is a sealed container capable of withstanding a vacuum, and the tank lid 2 is detachably and sealingly installed on the top of the tank body 1.

[0025] A heating jacket 3 is provided on the outside of the tank body 1. The heating jacket 3 has a heat medium inlet at the bottom and a heat medium outlet at the top. Hot water or low-pressure steam is introduced into the heating jacket 3 to indirectly heat the fresh chicken bile in the tank 1.

[0026] A drive motor 4 is installed on the can lid 2. The output end of the drive motor 4 is connected to the stirring shaft 5. The drive motor 4 is an explosion-proof motor. The stirring shaft 5 and the can lid 2 adopt a mechanical seal structure suitable for vacuum and ethanol environments.

[0027] The stirring shaft 5 is arranged along the central axis of the tank body 1. The wall scraper 6 includes a transverse connecting part and wall scraping parts disposed at both ends of the transverse connecting part. The transverse connecting part is fixedly connected to the stirring shaft 5. The two wall scraping parts extend vertically along the inner wall of the tank body 1 and maintain a scraping gap or elastic contact with the inner wall of the tank body 1.

[0028] The spiral blade 7 is fixedly connected to the outer periphery of the stirring shaft 5 and located between the two scraper sections. When the stirring shaft 5 rotates, the scraper 6 scrapes off the material adhering to the inner wall of the tank 1, and the spiral blade 7 pushes the material at different heights to generate axial flow, preventing the material from rotating only circumferentially with the stirring shaft 5.

[0029] A discharge pipe 8 is provided at the bottom of the tank body 1, and the discharge pipe 8 is connected to the inlet of the three-way valve 10. The first outlet of the three-way valve 10 is connected to the inlet of the circulating pump 9, and the outlet of the circulating pump 9 is connected to the annular distributor 12 through the circulating pipe 11. The second outlet of the three-way valve 10 forms a discharge branch, which is used to transport the material in the tank body 1 to an external centrifuge or receiving container.

[0030] The annular feeder 12 is arranged circumferentially along the inner wall of the tank 1. The lower part of the annular feeder 12 is provided with multiple feeding holes, which are distributed at intervals along the circumference of the annular feeder 12 and are inclined toward the inner wall of the tank 1 respectively.

[0031] During vacuum concentration, the first outlet of the three-way valve 10 is opened and the second outlet is closed. The circulation pump 9 draws material from the bottom of the tank 1 and transports the material to the annular distributor 12 via the circulation pipe 11. The material flows through multiple distribution holes to the inner wall of the tank 1 and forms a downward-flowing material layer along the inner wall of the tank 1.

[0032] The tank cover 2 is connected to the condenser 14 via the steam pipe 13, and the condensate outlet of the condenser 14 is connected to the collection tank 15. The installation height of the collection tank 15 is higher than that of the annular distributor 12, and the bottom of the collection tank 15 is connected to the annular distributor 12 via the return pipe 16.

[0033] The collection tank 15 is also connected to a recovery pipe 17, and control valves are respectively installed on the return pipe 16 and the recovery pipe 17. The collection tank 15 is equipped with a vacuum interface 18, which is connected to a vacuum device, so that the tank body 1, the steam pipe 13, the condenser 14 and the collection tank 15 form a closed pressure reduction system.

[0034] During ethanol reflux extraction, the reflux pipe 16 is opened and the recovery pipe 17 is closed. The ethanol vapor generated in the tank 1 enters the condenser 14 through the vapor pipe 13, condenses into liquid ethanol, and then enters the collection tank 15. Since the collection tank 15 is higher than the annular distributor 12, the condensed ethanol enters the annular distributor 12 through the reflux pipe 16 under the action of gravity and flows downward along the inner wall of the tank 1.

[0035] The refluxed condensed ethanol can wash away the adhering material on the inner wall of tank 1 and gradually enter the concentrated chicken bile paste, so that the ethanol and the concentrated paste come into uniform contact, reducing local agglomeration caused by a one-time concentrated addition of ethanol.

[0036] When recovering ethanol, the reflux pipe 16 is closed and the recovery pipe 17 is opened. Ethanol vapor is condensed by the condenser 14 and enters the collection tank 15, and then enters the sealed ethanol receiving container through the recovery pipe 17. By changing the on / off state of the reflux pipe 16 and the recovery pipe 17, the ethanol reflux state and the ethanol recovery state can be switched without disassembling the pipeline.

[0037] The tank 1, vapor pipe 13, condenser 14, collection tank 15, return pipe 16, and recovery pipe 17, which come into contact with ethanol in the device, are all connected in a sealed manner and are equipped with electrostatic grounding. Before adding ethanol into tank 1, the air inside tank 1 can be replaced with nitrogen to reduce the possibility of ethanol vapor forming an explosive mixture with air.

[0038] Working principle: At the start of the preparation process, filtered fresh chicken bile is added to tank 1. The vacuum equipment, heating jacket 3, and drive motor 4 are then activated to concentrate the fresh chicken bile under reduced pressure and stirring conditions.

[0039] As the water content in the fresh chicken bile decreases, the viscosity of the material gradually increases. At this point, the circulation pump 9 is turned on, so that the material at the bottom of the tank 1 is transported to the annular distributor 12 through the circulation pipe 11 and flows down the inner wall of the tank 1 again.

[0040] The scraper 6 rotates with the stirring shaft 5 to scrape off the adhering material on the inner wall of the tank 1; the spiral blades 7 push the material to axially tumble; the circulating material flows downward along the inner wall of the tank 1 and carries away the material close to the heating wall. The combination of these three functions can reduce the retention of highly viscous materials on the heating wall.

[0041] After concentrating fresh chicken bile to a moisture content of no more than 42.24%, the circulation pump 9 is turned off. The resulting concentrated paste is not vacuum dried, pulverized, or sieved; instead, 95% ethanol is added directly based on the mass of the dry solids it contains.

[0042] During the reduced pressure reflux extraction process, ethanol vapor is condensed by condenser 14 and enters the collection tank 15, then returns to the tank body 1 through reflux pipe 16 and annular distributor 12. After the first reflux extraction is completed, centrifugation is performed, and ethanol is added again to the separated residue for a second reflux extraction.

[0043] The extracts obtained from the two centrifugation separations were combined, the reflux tube 16 was closed and the recovery tube 17 was opened, and the ethanol was recovered at a temperature not exceeding 60°C. After the ethanol recovery was completed, vacuum concentration was carried out to obtain fresh chicken bile paste.

[0044] Example 1: Direct ethanol reflux extraction of concentrated paste Take 7.680 kg of fresh chicken bile, filter it through a No. 5 sieve, and add it to tank 1. The water content of the fresh chicken bile is 89.80%, and the chenodeoxycholic acid content is 18.2%, on a dry basis.

[0045] Start the drive motor 4 to rotate the stirring shaft 5, the scraper frame 6, and the spiral blades 7. Evacuate the tank 1 through the vacuum port 18 and introduce hot water into the heating jacket 3, controlling the water bath temperature to 70℃, while ensuring the material temperature does not exceed 70℃.

[0046] During the concentration process, the circulation pump 9 is turned on, so that the material at the bottom of the tank 1 is transported to the annular distributor 12 through the circulation pipe 11, and then circulates along the inner wall of the tank 1 through the annular distributor 12.

[0047] After concentration, 1.372 kg of concentrated paste was obtained, with a moisture content of 42.24% and a solid content of 57.76%. The concentrated paste was not dried or pulverized and was directly used for ethanol reflux extraction.

[0048] Take 207.8g of concentrated paste, the mass of which is approximately: 207.8 × 57.76% = 120.03g.

[0049] Based on the calculation of adding 10 mL of ethanol per gram of dry solids, 1200 mL of 95% ethanol was added for the first extraction. The mixture was refluxed under reduced pressure for 1.5 h, with the material temperature controlled not to exceed 70℃. After the first reflux extraction, centrifugation was performed to obtain the first supernatant and the first residue.

[0050] Add 1200 mL of 95% ethanol to the residue from the first extraction and perform a second reflux extraction under reduced pressure under the same conditions for 1.5 h. After the second reflux extraction, centrifuge to obtain the second supernatant.

[0051] The first and second supernatants were combined, and ethanol was recovered at 60°C. The supernatants were then concentrated and dried under vacuum to obtain 98.5g of alcohol extract for quality testing.

[0052] The 207.8g of concentrated extract was equivalent to 1163.1g of fresh chicken bile. Based on the corresponding mass of fresh chicken bile, the yield was 8.47%. The chenodeoxycholic acid content in the obtained alcohol extract was 26.1%.

[0053] The above results indicate that concentrated chicken bile extract with a moisture content of 42.24% can be directly extracted by ethanol reflux without prior drying and pulverization.

[0054] Comparative Example 1: Ethanol reflux extraction of chicken bile extract Chicken bile concentrate was vacuum dried at a temperature below 70°C, then pulverized and passed through a No. 2 sieve to obtain dry chicken bile extract.

[0055] Take 101.2g of dried chicken bile extract, with a moisture content of 3.5%, the mass of its dry solids is approximately: 101.2 × (1 - 3.5%) = 97.66 g.

[0056] Based on the calculation of adding 10 mL of ethanol per gram of dry solids, the first addition was approximately 977 mL of 95% ethanol. The mixture was then refluxed under reduced pressure for 1.5 h, followed by centrifugation after extraction.

[0057] Add approximately 977 mL of 95% ethanol to the residue obtained from the first centrifugation and perform a second vacuum reflux extraction under the same conditions for 1.5 h, followed by centrifugation.

[0058] The supernatants obtained from the two centrifugations were combined, and the ethanol was recovered at 60°C and dried under vacuum to obtain 86.5g of alcohol extract.

[0059] The 101.2g of dried chicken bile extract is equivalent to 931.1g of fresh chicken bile. Based on the corresponding mass of fresh chicken bile, the yield is 9.29%. The chenodeoxycholic acid content in the obtained alcohol extract is 24.9%.

[0060] Comparison of test results In Example 1, a concentrated paste with a water content of 42.24% was directly extracted by ethanol reflux. The resulting dry extract contained 26.1% chenodeoxycholic acid and had a yield of 8.47%.

[0061] In Comparative Example 1, chicken bile concentrate was pre-dried, pulverized, and then extracted by ethanol reflux. The resulting ethanol extract contained 24.9% chenodeoxycholic acid and had a yield of 9.29%.

[0062] The chenodeoxycholic acid content and yield of the alcohol-extracted dry extracts obtained from the two groups of experiments did not show significant differences, indicating that under the process conditions specified in this invention, when the moisture content of the concentrated chicken bile extract is not higher than 42.24%, the drying, pulverizing and sieving steps can be omitted, and ethanol can be directly added for vacuum reflux extraction.

[0063] The content of chenodeoxycholic acid in the ethanol extract obtained from the direct ethanol extraction group was not lower than that in the dry extract feeding group, indicating that the moisture content of the concentrated ethanol extract did not have a significant adverse effect on the ethanol reflux extraction under the above conditions.

[0064] Chicken Fresh Bile Extract Quality Adaptation Instructions The prescription for pertussis tablets contains 65g of fresh chicken bile extract, 15g of calcium carbonate, 30g of starch, 0.033g of ethylparaben, and 1.1g of magnesium stearate per 1000 tablets, with each tablet weighing 0.1g.

[0065] Based on a total weight of 100g for 1000 tablets and a moisture content of 5%, the total mass of excipients and moisture is: 15+30+0.033+1.1+100×5%=51.133g.

[0066] The theoretical mass of the dry solids derived from fresh chicken bile extract in the preparation is: 100 - 51.133 = 48.867g.

[0067] Based on a feed intake of 65g of fresh chicken bile extract, its theoretical moisture content is: (65-48.867)÷65×100%=24.82%.

[0068] Each tablet must contain no less than 6.0 mg of chenodeoxycholic acid, therefore, the total amount of chenodeoxycholic acid in 1000 tablets must be no less than 6.0 g. The theoretical lower limit of chenodeoxycholic acid content in fresh chicken bile extract dried solids is: 6.0 ÷ 48.867 × 100% = 12.28%.

[0069] The chenodeoxycholic acid content in the alcohol extract obtained in Example 1 was 26.1%. Based on the theoretical moisture content of 24.82% for fresh chicken bile extract, the chenodeoxycholic acid content is approximately: 26.1% × (1 - 24.82%) = 19.62%.

[0070] The chenodeoxycholic acid content in the alcohol extract obtained in Comparative Example 1 was 24.9%. Adjusted for the same moisture content, the chenodeoxycholic acid content is approximately: 24.9% × (1 - 24.82%) = 18.72%.

[0071] The above conversion results are all higher than the quality requirement of chenodeoxycholic acid content of not less than 15.0% in fresh chicken bile extract, indicating that the fresh chicken bile extract prepared by the method of the present invention can meet the raw material quality requirements of the corresponding preparation.

[0072] It should be noted that the 24.82% mentioned above is the target moisture content of fresh chicken bile extract obtained through theoretical calculation based on the formulation formula and moisture content, and is not the moisture data directly detected in the examples. In actual production, the concentration endpoint and moisture content should be detected and controlled according to the quality standards for fresh chicken bile extract.

[0073] The above description is merely a preferred embodiment of the invention, but the scope of protection of the invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the invention, based on the technical solution and inventive concept of the invention, should be included within the scope of protection of the invention.

Claims

1. A device for preparing fresh chicken bile paste, comprising a tank (1) and a lid (2) disposed on the top of the tank (1), characterized in that: A heating jacket (3) is provided on the outside of the tank body (1), and a drive motor (4) is provided on the tank cover (2). The output end of the drive motor (4) is connected to a stirring shaft (5) that extends into the tank body (1). A wall scraper (6) and a spiral blade (7) are respectively connected to the stirring shaft (5). A discharge pipe (8) is provided at the bottom of the tank body (1). The discharge pipe (8) is connected to the inlet of a three-way valve (10). The first outlet of the three-way valve (10) is circulated sequentially. The pump (9) and the circulation pipe (11) are connected to the annular distributor (12) located on the upper inner side of the tank body (1). The second outlet of the three-way valve (10) forms a discharge branch. The tank cover (2) is connected to the condenser (14) through the steam pipe (13). The condensate outlet of the condenser (14) is connected to the collection tank (15). The collection tank (15) is connected to the return pipe (16) and the recovery pipe (17). The return pipe (16) is connected to the annular distributor (12).

2. The apparatus for preparing fresh chicken bile paste according to claim 1, characterized in that: The scraper (6) includes a transverse connecting part connected to the stirring shaft (5) and scraper parts disposed at both ends of the transverse connecting part. The scraper parts extend vertically along the inner wall of the tank (1) and are in clearance fit or elastic contact with the inner wall of the tank (1). The spiral blade (7) is located between the two scraper parts.

3. The apparatus for preparing fresh chicken bile paste according to claim 1, characterized in that: The annular feeder (12) is arranged around the circumference of the tank (1). The lower part of the annular feeder (12) is provided with a plurality of feed holes facing the inner wall of the tank (1). The plurality of feed holes are distributed at intervals around the circumference of the annular feeder (12).

4. The apparatus for preparing fresh chicken bile paste according to claim 1, characterized in that: The circulation pipe (11) and return pipe (16) are respectively connected to the independent liquid inlet on the annular distributor (12), and the circulation pipe (11) and return pipe (16) are respectively equipped with one-way valves to prevent fluid backflow.

5. The apparatus for preparing fresh chicken bile paste according to claim 1, characterized in that: The installation height of the collection tank (15) is higher than that of the annular distributor (12), and the return pipe (16) is connected to the bottom of the collection tank (15) so that the condensed ethanol in the collection tank (15) enters the annular distributor (12) under the action of gravity.

6. The apparatus for preparing fresh chicken bile paste according to claim 1, characterized in that: The collection tank (15) is provided with a vacuum interface (18) for connecting to a vacuum device. The return pipe (16) and the recovery pipe (17) are respectively provided with control valves. The recovery pipe (17) is connected to a sealed ethanol receiving container.

7. A method for preparing fresh chicken bile extract using the apparatus according to any one of claims 1-6, characterized in that, Includes the following steps: S1. After filtering the fresh chicken bile, add it to the tank (1). Vacuum the tank (1) through the vacuum interface (18) and indirectly heat the fresh chicken bile through the heating jacket (3). Vacuum concentrate the material at a temperature not higher than 70°C until the moisture content of the concentrated paste is not higher than 42.24%. S2. Without drying and pulverizing the concentrated paste, add 10 mL of 95% ethanol per gram of dry solids according to the mass of the concentrated paste, and reflux extract under reduced pressure for 1.5 h at a material temperature not exceeding 70°C. After the first reflux extraction is completed, perform solid-liquid separation. S3. Add 95% ethanol to the residue obtained from the first solid-liquid separation at 10 mL per gram of the initial dry solids. Reflux extract under reduced pressure for 1.5 h at a material temperature not exceeding 70°C. After the second reflux extraction is completed, perform solid-liquid separation and combine the extracts obtained from the two solid-liquid separations. S4. Close the reflux tube (16) and open the recovery tube (17). Recover the ethanol in the extract at a temperature not higher than 60°C. Vacuum concentrate the extract after ethanol recovery into a thick paste to obtain fresh chicken bile paste.

8. The method for preparing fresh chicken bile extract according to claim 7, characterized in that: During the vacuum concentration process in step S1, the circulation pump (9) is turned on, so that the material at the bottom of the tank (1) passes through the discharge pipe (8), the three-way valve (10), the circulation pump (9) and the circulation pipe (11) in sequence and enters the annular distributor (12), and is distributed downward along the inner wall of the tank (1) through the annular distributor (12).

9. The method for preparing fresh chicken bile extract according to claim 7, characterized in that: During the depressurized reflux extraction process in steps S2 and S3, the circulation pump (9) is turned off, and the ethanol vapor is condensed in sequence through the steam pipe (13) and the condenser (14) and then enters the collection tank (15), and then returns to the concentrated paste along the inner wall of the tank (1) through the reflux pipe (16) and the annular distributor (12); the solid-liquid separation is carried out by centrifugal separation.

10. The method for preparing fresh chicken bile extract according to claim 7, characterized in that: The chenodeoxycholic acid content of the fresh chicken bile extract obtained in step S4 shall not be less than 15.0%.

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