Modular multi-component quantitative liquid dispensing microfluidic chip and liquid dispensing method

Abstract

The application discloses a modular multi-component quantitative liquid dispensing micro-fluidic chip and a liquid dispensing method. The chip comprises a driving member, a plurality of quantitative disc modules stacked along an axial direction and a base. The quantitative disc module comprises a fixed outer ring and a rotating inner disc with a quantitative cavity. An interlayer sealing part and a convex ridge for layer-by-layer transmission are arranged between adjacent rotating inner discs. The driving member drives the uppermost rotating inner disc to rotate. The friction and the convex ridge abutment make each rotating inner disc sequentially isolated, connected and form a linkage disc group. The linkage disc group rotates continuously at a preset rotating speed in the same direction, so that the liquid inlet valve plate and the liquid overflow valve plate remain closed when passing through the corresponding alignment area. The following bottom cover rotates with the linkage disc group during mixing and keeps the liquid outlet hole misaligned. After locking, the liquid outlet hole is aligned with the liquid outlet. The application can realize modular quantitative, layer-by-layer connection, closed mixing and delayed liquid outlet.

Description

Technical Field

[0001] This invention belongs to the field of microfluidics technology, specifically relating to a modular multi-component quantitative liquid preparation microfluidic chip and liquid preparation method. Background Technology

[0002] Microfluidic chips can perform operations such as liquid delivery, quantification, mixing and reaction in microscale channels and chambers, and have been applied in fields such as biological detection, chemical analysis and sample processing.

[0003] In multi-component liquid distribution processes, it is usually necessary to quantify each component separately, and then combine, mix, and output the liquids. Existing technologies mostly use independent inlet channels in conjunction with pumps, valves, or fixed-volume chambers to achieve liquid delivery and quantification. Some chips change the direction of liquid flow by sliding, rotating, or switching valve paths.

[0004] However, the quantitative cavity volume, number of flow channels, and connection relationships of existing microfluidic chips are usually fixed. When the number or ratio of components changes, it is often necessary to redesign the chip or add external pipelines and control components, resulting in poor adaptability. With the increase of the number of components, problems such as more drive interfaces, more complex flow path switching, and reduced sealing reliability are also likely to occur.

[0005] Furthermore, existing multi-component microfluidic dispensing structures typically require independent mixing and dispensing control structures after each component has been quantified and connected, making it difficult to simultaneously ensure closed-loop maintenance during continuous mixing and reliable dispensing after mixing. Therefore, existing multi-component microfluidic dispensing technologies still have room for improvement in terms of module selection, sequential connection of multiple quantitative units, and the connection between mixing and dispensing. Summary of the Invention

[0006] The present invention aims to provide a modular multi-component quantitative liquid preparation microfluidic chip and liquid preparation method to solve the problems mentioned in the background art.

[0007] To achieve the above objectives, the present invention adopts the following technical solution.

[0008] This invention provides a modular multi-component quantitative liquid dispensing microfluidic chip, including a driving component and multiple quantitative disk modules stacked along the axial direction. Each quantitative disk module includes a fixed outer ring and a rotating inner disk rotatably disposed within the fixed outer ring. The fixed outer ring is provided with an inlet channel and an overflow cavity. The rotating inner disk is provided with a quantitative cavity inside, and its outer peripheral wall is provided with an inlet and an overflow port respectively communicating with the quantitative cavity. The upper and lower end faces are respectively provided with an upper connecting port and a lower connecting port communicating with the quantitative cavity. The rotating inner disk can rotate relative to the fixed outer ring to switch the metering cavity between a metering state and an isolation state; an interlayer sealing part is provided between adjacent rotating inner disks. In the initial state, the adjacent rotating inner disks are offset along the circumference, so that the lower connecting port of the upper rotating inner disk and the upper connecting port of the lower rotating inner disk are in an offset closed state, and can be aligned and connected as the rotating inner disk rotates. Each of the rotating inner disks has an upper protrusion and a lower protrusion on its upper and lower end faces, respectively. The upper protrusion and the lower protrusion are located in the transmission area on the opposite side of the metering cavity. The driving component is connected to the uppermost rotating inner disk. When the driving component drives the uppermost rotating inner disk to rotate, the adjacent rotating inner disks follow and rotate relative to each other through the friction of the interlayer sealing part, causing the lower rotating inner disk to enter an isolated state; the upper rotating inner disk continues to rotate relative to the lower rotating inner disk until the lower end protrusion of the upper rotating inner disk abuts against the upper end protrusion of the lower rotating inner disk, and pushes each rotating inner disk from top to bottom through the adjacent protrusions, so that the adjacent connecting ports are aligned in sequence and form a linkage disk group.

[0009] Optionally, in the metering state, the liquid inlet channel, liquid inlet, metering chamber, overflow port, and overflow chamber are connected in sequence; In the isolated state, the inlet is misaligned with the inlet channel, and the overflow outlet is misaligned with the overflow chamber, thereby isolating the metering chamber from the inlet channel and the overflow chamber.

[0010] Optionally, the upper and lower end faces of the fixed outer ring are axially recessed relative to the corresponding end faces of the rotating inner disk, and the interlayer sealing part protrudes from the upper and lower end faces of the rotating inner disk, and the interlayer sealing parts of adjacent metering disk modules are directly pressed together. The fixed outer ring is arranged around the rotating inner disk, and the inner circumferential surface of the fixed outer ring is rotatably engaged with the outer circumferential surface of the rotating inner disk.

[0011] Optionally, an inlet valve plate fixed to the fixed outer ring is provided between the inlet channel and the inlet, and an overflow valve plate fixed to the rotating inner disk is provided between the overflow port and the overflow chamber. The fixed ends of the inlet valve plate and the overflow valve plate are arranged in opposite directions along the circumference. The inlet valve and the overflow valve open under the injection pressure and close under the action of the corresponding mating surfaces during the process of the rotating inner disk changing from the metering state to the isolation state. The rotating inner disk rotates in the same direction at a preset speed during the subsequent mixing process, so that the duration of each time the inlet and the overflow port pass through the corresponding alignment area is less than the response time required for the corresponding valve to open from the closed state to form an effective flow gap, so that the inlet valve and the overflow valve remain closed during the mixing process.

[0012] Optionally, the upper and lower protrusions are respectively long strip protrusions extending radially or narrow fan-shaped protrusions; The lower end protrusion of the upper rotating inner disk and the upper end protrusion of the lower rotating inner disk have an overlapping area in the axial direction, and transmit torque by abutting against the opposite sidewalls along the circumferential direction.

[0013] Optionally, the number of quantitative disk modules and the volume of each quantitative chamber are selected according to the predetermined number of components and the component distribution ratio.

[0014] Optionally, it also includes a base located below the lowest quantitative plate module, the base including a fixed bottom shell and a follower bottom cover rotatably disposed within the fixed bottom shell; The follower bottom cover is provided with a liquid outlet that can be aligned with the lower connecting port of the lowest rotating inner disk and a cantilever elastic piece that can abut against the lower end protrusion of the lowest rotating inner disk. The fixed bottom shell is provided with a bottom cover locking member for locking the follower bottom cover. When the follower bottom cover is in a free rotation state, the lower end protrusion of the lowest rotating inner disk drives the follower bottom cover to rotate together with the linkage disk assembly through the cantilever elastic sheet, so that the lower connecting port of the lowest rotating inner disk is misaligned with the liquid outlet. After the follower bottom cover is locked by the bottom cover locking member, the linkage disk assembly can continue to rotate relative to the follower bottom cover, so that the lower end protrusion of the lowest rotating inner disk pushes against and passes over the cantilever elastic plate until the lower connecting port is aligned with the liquid outlet.

[0015] Optionally, the cantilever elastic plate includes a fixed end fixed to the follower bottom cover and a free end extending toward the lowest rotating inner disk, the free end being provided with a guide slope that cooperates with the lower end protrusion.

[0016] Optionally, the torque required for the free rotation of the follower bottom cover is less than the torque required for the cantilever elastic sheet to undergo over-deformation, and the locking torque provided by the bottom cover locking member is greater than the torque required for the cantilever elastic sheet to undergo over-deformation.

[0017] This invention also provides a method for preparing solutions using the aforementioned modular multi-component quantitative liquid preparation microfluidic chip, comprising the following steps: S1. Select multiple quantitative disk modules according to the predetermined number of components and the component ratio and stack them along the axial direction so that each rotating inner disk is in a quantitative state relative to the corresponding fixed outer ring, and make adjacent rotating inner disks misaligned along the circumferential direction, with the corresponding connecting port and corresponding protrusion in a misaligned state. S2. The corresponding components are injected into the liquid inlet channels of each metering plate module, so that the liquid enters the metering chamber and then enters the overflow chamber after the metering chamber is filled. S3. The uppermost rotating inner disk is driven to rotate by the driving component, so that the adjacent rotating inner disks follow each other and rotate relative to each other through the friction of the interlayer sealing part, so that each rotating inner disk enters the isolation state in sequence. S4. Make the lower end protrusion of the upper rotating inner disk abut against the upper end protrusion of the lower rotating inner disk in sequence, and push each rotating inner disk to rotate in sequence through the adjacent protrusions, so that the adjacent connecting ports are aligned in sequence and form a linkage disk group. S5. The follower bottom cover is in a free rotation state. The lower end protrusion of the bottom rotating inner disk drives the follower bottom cover to rotate together with the linkage disk assembly through the cantilever elastic plate, so that the lower connecting port of the bottom rotating inner disk is misaligned with the liquid outlet. The drive unit drives the linkage disk assembly to rotate continuously in the same direction at a preset speed, so that the duration of each time the liquid inlet and the overflow port pass through the corresponding alignment area is less than the response time required for the liquid inlet valve and the overflow valve to open from the closed state to form an effective flow gap, so that the liquid inlet valve and the overflow valve remain closed, and drive the linkage disk assembly to rotate a preset number of times to mix the components in each metering chamber. S6. After mixing is completed, the bottom cover is locked by the bottom cover locking device, so that the linkage disk group continues to rotate relative to the bottom cover, so that the lower end protrusion of the bottom rotating inner disk pushes against and passes over the cantilever elastic plate until the lower connecting port of the bottom rotating inner disk is aligned with the liquid outlet, and the mixed liquid is discharged through the lower connecting port and the liquid outlet.

[0018] Compared with the prior art, the present invention has at least the following beneficial effects: 1. This invention employs multiple quantitative disk modules stacked along the axial direction, and selects different numbers of quantitative disk modules and quantitative cavities of different volumes according to the predetermined number of components and component ratios to realize multi-component quantitative liquid preparation and improve the chip's adaptability to different liquid preparation requirements.

[0019] 2. This invention utilizes the friction of the interlayer sealing parts to cause adjacent rotating inner disks to follow and rotate relative to each other, causing the lower rotating inner disk to enter an isolated state; subsequently, the protrusions of the adjacent rotating inner disks abut against each other to directly push them, so that each rotating inner disk completes isolation and connection from top to bottom in sequence, reducing the possibility of abnormal connection caused by positional displacement during the transmission of multiple rotating inner disks.

[0020] 3. The present invention provides an upper protrusion and a lower protrusion on the upper and lower end faces of the rotating inner disk, and positions the protrusions in the transmission area on the opposite side of the metering cavity. The adjacent protrusions sequentially abut against and push each rotating inner disk, thereby realizing the layer-by-layer access and linkage of multiple metering disk modules.

[0021] 4. This invention utilizes the engagement of the lower end protrusion of the bottom rotating inner disc with the cantilever elastic plate on the follower bottom cover, allowing the follower bottom cover to rotate freely and together with the linkage disc assembly. This also ensures that the lower connecting port of the bottom rotating inner disc is misaligned with the liquid outlet of the follower bottom cover. After mixing is complete and the follower bottom cover is locked, the lower end protrusion pushes against the cantilever elastic plate, causing elastic deformation and passing over the cantilever elastic plate. This allows the bottom rotating inner disc to continue rotating relative to the follower bottom cover until the lower connecting port aligns with the liquid outlet, achieving closed-loop maintenance during mixing and liquid outlet switching after mixing, thus preventing premature liquid outlet during mixing.

[0022] 5. The present invention enables the linkage disk assembly to rotate continuously in the same direction at a preset speed, shortening the duration of each passage of the liquid inlet and overflow port through the corresponding alignment area, so that the liquid inlet valve plate and the overflow valve plate remain closed during the mixing process, and preventing the metering chamber from re-establishing effective communication with the liquid inlet channel or the overflow chamber during continuous rotation and mixing. Attached Figure Description

[0023] Figure 1 This is a schematic diagram of the overall structure of the modular multi-component quantitative liquid preparation microfluidic chip of the present invention; Figure 2 This is a schematic diagram of the quantitative disk module of the present invention; Figure 3 This is a schematic diagram of the structure of the base of the present invention; Figure 4 for Figure 3 Enlarged structural diagram at point A; Figure 5 This is a schematic diagram showing the installation positions of the inlet valve plate and the overflow valve plate of the present invention.

[0024] In the diagram: 1. Metering disc module; 11. Fixed outer ring; 111. Liquid inlet channel; 112. Overflow chamber; 12. Rotating inner disc; 121. Metering chamber; 122. Liquid inlet; 123. Overflow port; 124. Upper connecting port; 125. Lower connecting port; 126. Upper protrusion; 127. Lower protrusion; 13. Interlayer sealing part; 2. Driving component; 3. Base; 31. Fixed bottom shell; 32. Follow-up bottom cover; 321. Liquid outlet; 322. Cantilever elastic plate; 3221. Fixed end; 3222. Free end; 3223. Guide slope; 33. Bottom cover locking component; 4. Liquid inlet valve plate; 5. Overflow valve plate. Detailed Implementation

[0025] The specific embodiments of the present invention will be further described below with reference to the accompanying drawings. It should be understood that the following embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention; those skilled in the art can make equivalent substitutions or conventional modifications to the components without departing from the concept of the present invention.

[0026] See Figure 1 This embodiment provides a modular multi-component quantitative liquid dispensing microfluidic chip, including a driving component 2 and multiple quantitative disk modules 1 stacked sequentially along the axial direction. In this embodiment, a base 3 is also provided below the bottommost quantitative disk module 1, which is used to close the liquid outlet passage during the mixing process and open the liquid outlet passage after the mixing is completed.

[0027] Each metering disc module 1 may have the same or different metering chambers 121. In use, the appropriate number of metering disc modules 1 and the volume of each metering chamber 121 are selected according to the number of components and the predetermined component ratio. For example, when the ratio of the three components is 1:1:1, three metering disc modules 1 with the same volume of metering chambers 121 can be selected; when the ratio is 1:2:3, three metering disc modules 1 with metering chamber volumes corresponding to the ratio can be selected.

[0028] See Figure 2 and Figure 5 Each quantitative disk module 1 includes a fixed outer ring 11 and a rotating inner disk 12 rotatably disposed within the fixed outer ring 11. The inner circumferential surface of the fixed outer ring 11 rotatably engages with the outer circumferential surface of the rotating inner disk 12. Adjacent fixed outer rings 11 are assembled with a predetermined circumferential angle offset and can be connected to positioning posts, circumferential slots, or other anti-rotation structures through positioning holes, so that multiple fixed outer rings 11 remain relatively fixed during chip operation.

[0029] The rotating inner disk 12 has a metering cavity 121 inside, which can be a fan-shaped cavity, an arc-shaped cavity, or other fixed-volume cavity. In one embodiment, the metering cavity 121 is a fan-shaped cavity with a central angle of approximately 40°. The outer peripheral wall of the rotating inner disk 12 has an inlet 122 and an overflow port 123 that communicate with the metering cavity 121, respectively. The fixed outer ring 11 has an inlet flow channel 111 corresponding to the inlet 122 and an overflow cavity 112 corresponding to the overflow port 123. The overflow cavity 112 can be an arc-shaped cavity or other cavity structure capable of accommodating overflowing liquid.

[0030] The rotating inner disk 12 can switch between a metering state and an isolation state relative to the fixed outer ring 11. In the metering state, the inlet channel 111, inlet port 122, metering chamber 121, overflow port 123, and overflow chamber 112 are sequentially connected. The corresponding component enters the metering chamber 121 through the inlet channel 111 and inlet port 122. After the metering chamber 121 is full, the excess liquid enters the overflow chamber 112 through the overflow port 123, so that the volume of liquid retained in the metering chamber 121 is determined by the volume of the metering chamber 121. The overflow chamber 112 may be provided with a vent to allow the gas in the metering chamber 121 and the overflow chamber 112 to be discharged during the liquid injection process.

[0031] In the isolated state, the inlet 122 is misaligned with the inlet channel 111, and the overflow outlet 123 is misaligned with the overflow chamber 112, thus isolating the metering chamber 121 from the inlet channel 111 and the overflow chamber 112.

[0032] To facilitate the reset of the metering disc module 1 after use, corresponding alignment marks are provided on the same side of the fixed outer ring 11 and the rotating inner disc 12. These alignment marks can be engraved lines, grooves, raised areas, or arrows. When the rotating inner disc 12 rotates relative to the fixed outer ring 11 until the two alignment marks are aligned, the liquid inlet channel 111 connects to the liquid inlet 122, and the overflow port 123 connects to the overflow chamber 112, restoring the rotating inner disc 12 to its metering state. After dispensing, each rotating inner disc 12 can be reset according to the alignment marks for subsequent reassembly and use.

[0033] In the initial assembly state, each rotating inner disk 12 is in a quantitative state relative to its corresponding fixed outer ring 11. Since the adjacent fixed outer rings 11 are assembled with a predetermined circumferential angle offset, the adjacent rotating inner disks 12 are also offset circumferentially, so that the lower connecting port 125 of the upper rotating inner disk 12 and the upper connecting port 124 of the lower rotating inner disk 12 are in an offset closed state, preventing the quantitative chambers 121 from connecting prematurely during the liquid injection stage.

[0034] See Figure 5 An inlet valve plate 4 is fixed to the fixed outer ring 11 between the inlet channel 111 and the inlet port 122, and an overflow valve plate 5 is fixed to the rotating inner disk 12 between the overflow port 123 and the overflow chamber 112. Both the inlet valve plate 4 and the overflow valve plate 5 can be made of flexible thin sheet structure, and their fixed ends are arranged in opposite directions along the circumference.

[0035] During liquid injection, the inlet valve 4 and the overflow valve 5 open under liquid pressure. When the inner disk 12 is rotated from the metering state to the isolation state, the inlet valve 4 and the overflow valve 5 close due to the action of their corresponding mating surfaces, so as to reduce the liquid in the metering chamber 121 from returning to the inlet channel 111 or continuing to enter the overflow chamber 112.

[0036] During the subsequent mixing process, the drive unit 2 drives the linkage disk group formed by each rotating inner disk 12 to rotate continuously in the same direction at a preset speed. Although the liquid inlet 122 and the overflow port 123 periodically pass through the alignment area corresponding to the liquid inlet channel 111 and the overflow chamber 112 with the rotating inner disk 12, the duration of each passage through the alignment area is less than the response time required for the liquid inlet valve plate 4 and the overflow valve plate 5 to open from the closed state to form an effective flow gap. Therefore, the liquid inlet valve plate 4 and the overflow valve plate 5 remain closed during the continuous rotation mixing process to prevent the metering chamber 121 from re-establishing an effective connection with the liquid inlet channel 111 or the overflow chamber 112.

[0037] The preset rotational speed can be determined based on the circumferential width of the corresponding alignment area and the material, thickness, free end length, and elastic recovery performance of the valve plate, so that the time for the inlet 122 or the overflow 123 to pass through the corresponding alignment area once is less than the opening response time of the corresponding valve plate. The preset rotational speed can be determined by calculation or pre-testing.

[0038] Each rotating inner disk 12 has an upper connecting port 124 and a lower connecting port 125 on its upper and lower end faces, respectively, which communicate with the metering cavity 121. When adjacent rotating inner disks 12 are in an initial misaligned state, the lower connecting port 125 of the upper rotating inner disk 12 is misaligned with the upper connecting port 124 of the lower rotating inner disk 12 and is closed by the mating surface between adjacent rotating inner disks 12. After the adjacent rotating inner disks 12 rotate relative to each other to an interlayer communication state, the lower connecting port 125 and the upper connecting port 124 are aligned, so that the adjacent metering cavities 121 are interconnected.

[0039] An interlayer sealing part 13 is provided between adjacent rotating inner disks 12. The interlayer sealing part 13 may be an annular sealing flange, a sealing lip, an elastic sealing ring, or a partial sealing boss. The upper and lower end faces of the fixed outer ring 11 are axially recessed relative to the corresponding end faces of the rotating inner disks 12, so that after the adjacent metering disk modules 1 are stacked, the interlayer sealing parts 13 on the adjacent rotating inner disks 12 are directly pressed together.

[0040] The chip casing or axial clamping structure can apply axial preload to multiple metering disk modules 1 to maintain the sealing contact of the interlayer sealing part 13 and to form a frictional effect between adjacent rotating inner disks 12.

[0041] When the upper rotating inner disk 12 rotates, the interlayer sealing part 13 drives the lower rotating inner disk 12 to move along with it through friction. Due to the rotational resistance between the lower rotating inner disk 12 and the fixed outer ring 11, the sealing contact resistance, and the load of the structure below, the adjacent rotating inner disks 12 rotate circumferentially relative to each other during the following process.

[0042] See Figure 2 Each rotating inner disk 12 has an upper protrusion 126 on its upper end face and a lower protrusion 127 on its lower end face. The upper protrusion 126 and lower protrusion 127 can be either long, radially extending protrusions or narrow, fan-shaped protrusions. The upper protrusion 126 and lower protrusion 127 are located in the transmission area on opposite sides of the metering cavity 121, and are situated on the same radial axis and axially corresponding. To facilitate simultaneous display of the upper protrusion 126 and lower protrusion 127 located on different end faces, Figure 2 The two are schematically staggered, but their actual circumferential positions correspond to each other along the axial direction.

[0043] In adjacent rotating inner disks 12, the lower end protrusion 127 of the upper rotating inner disk 12 and the upper end protrusion 126 of the lower rotating inner disk 12 have an overlapping area in the axial direction, and can transmit torque through the abutment of the opposite sidewalls along the circumferential direction. Since the adjacent rotating inner disks 12 are initially misaligned along the circumferential direction, the corresponding lower end protrusion 127 and upper end protrusion 126 are also in a circumferential misalignment state.

[0044] The axial protrusion height of the interlayer sealing part 13 creates a transmission gap between the corresponding end faces of adjacent rotating inner disks 12. The upper protrusion 126 and the lower protrusion 127 are located within the transmission gap and transmit torque through the circumferential sidewall.

[0045] The drive unit 2 is connected to the uppermost rotating inner disk 12 via a drive section, a plug-in shaft, or a drive groove. The drive unit 2 first drives the uppermost rotating inner disk 12 to rotate, and the lower rotating inner disk 12 follows under the friction of the interlayer sealing part 13, and rotates circumferentially relative to the uppermost rotating inner disk 12, so that the lower rotating inner disk 12 changes from a quantitative state to an isolated state.

[0046] The uppermost rotating inner disk 12 continues to rotate relative to the lower rotating inner disk 12 until its lower end protrusion 127 abuts against the upper end protrusion 126 of the lower rotating inner disk 12. The circumferential positions of adjacent protrusions and corresponding connecting ports are arranged so that when adjacent protrusions abut, the lower connecting port 125 of the uppermost rotating inner disk 12 is aligned with the upper connecting port 124 of the lower rotating inner disk 12.

[0047] After adjacent protrusions abut, the uppermost rotating inner disk 12 pushes the lower rotating inner disk 12 to rotate through the protrusions, so that the corresponding lower connecting port 125 and upper connecting port 124 are aligned. The rotating inner disks 12 that have established a protrusion transmission relationship rotate together, and continue to drive the next lower rotating inner disk 12 through the lower end protrusion 127 of the lower rotating inner disk 12.

[0048] The next layer of rotating inner disk 12 moves along with the interlayer sealing part 13 under friction and enters the isolation state. Then, its upper protrusion 126 abuts against the lower protrusion 127 of the upper layer of rotating inner disk 12, and at the same time, the corresponding upper connecting port 124 and lower connecting port 125 are aligned.

[0049] According to the above process, the adjacent protrusions abut against each rotating inner disk 12 from top to bottom and push them to rotate, and the corresponding adjacent connecting ports are aligned in sequence, finally forming a linkage disk group.

[0050] After the linkage disk assembly is formed, the drive component 2 drives the linkage disk assembly to rotate continuously in the same direction at a preset speed, so that the liquid in each connected metering chamber 121 flows and is mixed. The mixing time or number of rotations can be set according to the component volume, viscosity and mixing requirements.

[0051] See Figure 3 The base 3 includes a fixed base shell 31 and a follower base cover 32 rotatably disposed within the fixed base shell 31. The follower base cover 32 is coaxially disposed with the lowest rotating inner disk 12 and can rotate relative to the fixed base shell 31. The fixed base shell 31 may adopt a ring support structure, or an open area may be left in the drainage path of the liquid outlet 321 to avoid obstructing the drainage of the liquid outlet 321.

[0052] The follower bottom cover 32 is located below the lowest rotating inner disk 12, and its side facing the lowest rotating inner disk 12 is rotatably engaged with the lower end face of the lowest rotating inner disk 12. The follower bottom cover 32 is provided with a liquid outlet 321, and the lower connecting port 125 of the lowest rotating inner disk 12 also serves as the bottom liquid outlet. When the lower connecting port 125 is misaligned with the liquid outlet 321, the lower connecting port 125 is closed by the solid disk surface of the follower bottom cover 32; when the lower connecting port 125 is aligned with the liquid outlet 321, the bottom liquid outlet passage is opened.

[0053] See Figure 4 The follower bottom cover 32 is also provided with a cantilever elastic plate 322, which can abut against the lower end protrusion 127 of the lowest rotating inner disk 12. The cantilever elastic plate 322 includes a fixed end 3221 fixed to the follower bottom cover 32 and a free end 3222 extending toward the lowest rotating inner disk 12. The free end 3222 is provided with a guide slope 3223 that cooperates with the lower end protrusion 127.

[0054] The cantilever elastic sheet 322 can be made of metal or elastic polymer. When the lower protrusion 127 pushes against the guide slope 3223, the cantilever elastic sheet 322 can bend elastically to allow the lower protrusion 127 to pass over the cantilever elastic sheet 322.

[0055] See Figure 3 The side wall of the fixed base shell 31 is provided with a radially extending threaded hole. The bottom cover locking member 33 is a pre-tightening bolt threaded into the threaded hole, with the inner end of the pre-tightening bolt facing the outer peripheral surface of the follower bottom cover 32. When the pre-tightening bolt is loosened, its inner end separates from or releases pressure from the outer peripheral surface of the follower bottom cover 32, allowing the follower bottom cover 32 to rotate freely relative to the fixed base shell 31. When the pre-tightening bolt is tightened, its inner end presses against the outer peripheral surface of the follower bottom cover 32, applying a radial pre-tightening force to the follower bottom cover 32, and using the friction between the pre-tightening bolt and the follower bottom cover 32 to restrict the rotation of the follower bottom cover 32 relative to the fixed base shell 31.

[0056] To prevent the pre-tightening bolts from causing significant wear to the outer circumferential surface of the follower bottom cover 32, the inner end of the pre-tightening bolts may be provided with an elastic pressure pad, a friction pad, or an arc-shaped pressure head. The elastic pressure pad or friction pad may be made of rubber, silicone, or other materials with elastic and frictional properties.

[0057] When the pre-tightening bolts are in the loosened state, the torque required for the follower bottom cover 32 to rotate freely is less than the torque required for the cantilever elastic plate 322 to undergo over-deformation. Therefore, when the lower end protrusion 127 of the lowest rotating inner disk 12 pushes against the cantilever elastic plate 322, the cantilever elastic plate 322 remains in contact with the lower end protrusion 127 and drives the follower bottom cover 32 to rotate together.

[0058] During the mixing process, the follower bottom cover 32 rotates together with the linkage disk assembly. The circumferential relative position between the bottom rotating inner disk 12 and the follower bottom cover 32 remains unchanged, so that the lower connecting port 125 and the liquid outlet 321 are misaligned, and the bottom liquid outlet passage is in a closed state.

[0059] After mixing is complete, tighten the preload bolt so that its inner end presses against the outer circumferential surface of the follower bottom cover 32. The locking torque generated by the preload bolt on the follower bottom cover 32 is greater than the torque required to cause the cantilever elastic plate 322 to over-deform, thus keeping the follower bottom cover 32 in a locked state.

[0060] After the follower bottom cover 32 is locked, the drive unit 2 continues to drive the linkage disk assembly to rotate relative to the follower bottom cover 32. The lower end protrusion 127 of the bottom rotating inner disk 12 pushes against the cantilever elastic plate 322 along the guide slope 3223, causing the cantilever elastic plate 322 to bend and allowing the lower end protrusion 127 to pass over the cantilever elastic plate 322 until the lower connecting port 125 of the bottom rotating inner disk 12 is aligned with the liquid outlet 321 of the follower bottom cover 32.

[0061] After the lower connecting port 125 is aligned with the liquid outlet 321, the mixture is directly discharged through the lower connecting port 125 and the liquid outlet 321.

[0062] The solution preparation method in this embodiment includes the following steps: S1, select multiple quantitative disk modules 1 according to the predetermined number of components and the component ratio and stack them along the axial direction, so that each rotating inner disk 12 is in a quantitative state relative to the corresponding fixed outer ring 11, and make adjacent rotating inner disks 12 misaligned along the circumferential direction, with the corresponding connecting port and the corresponding protrusion in a misaligned state. S2, the corresponding components are injected through the liquid inlet channel 111 of each metering plate module 1, so that the liquid enters the metering chamber 121 and enters the overflow chamber 112 after filling the metering chamber 121; S3, the uppermost rotating inner disk 12 is driven to rotate by the driving component 2, so that the adjacent rotating inner disks 12 follow and rotate relative to each other through the friction of the interlayer sealing part 13, so that each rotating inner disk 12 sequentially enters the isolation state. S4, so that the lower end protrusion 127 of the upper rotating inner disk 12 abuts against the upper end protrusion 126 of the lower rotating inner disk 12 in sequence, and pushes each rotating inner disk 12 to rotate in sequence through the adjacent protrusions, so that the adjacent connecting ports are aligned in sequence and form a linkage disk group. S5, the pre-tightening bolts are loosened, allowing the follower bottom cover 32 to rotate freely relative to the fixed bottom shell 31; the lower end protrusion 127 of the lowest rotating inner disk 12 drives the follower bottom cover 32 to rotate together with the linkage disk assembly through the cantilever elastic plate 322, so that the lower connecting port 125 of the lowest rotating inner disk 12 is misaligned with the liquid outlet 321; the drive member 2 drives the linkage disk assembly to rotate continuously in the same direction at a preset speed, so that the duration of each passage of the liquid inlet 122 and the overflow port 123 through the corresponding alignment area is less than the response time required for the liquid inlet valve plate 4 and the overflow valve plate 5 to open from the closed state to form an effective flow gap, so that the liquid inlet valve plate 4 and the overflow valve plate 5 remain closed, and drive the linkage disk assembly to rotate a preset number of times to mix the components in each metering chamber 121; S6. After mixing is completed, tighten the pre-tightening bolt so that the inner end of the pre-tightening bolt presses against the outer circumferential surface of the follower bottom cover 32 and locks the follower bottom cover 32. Drive the linkage disc assembly to continue rotating relative to the follower bottom cover 32, so that the lower end protrusion 127 of the bottom rotating inner disc 12 pushes against and passes over the cantilever elastic plate 322 until the lower connecting port 125 of the bottom rotating inner disc 12 is aligned with the liquid outlet 321, and the mixture is discharged through the lower connecting port 125 and the liquid outlet 321.

Claims

1. A modular multi-component quantitative liquid dispensing microfluidic chip, comprising a driving component (2) and multiple quantitative disk modules (1) stacked along the axial direction, characterized in that: Each of the metering disc modules (1) includes a fixed outer ring (11) and a rotating inner disc (12) rotatably disposed within the fixed outer ring (11). The fixed outer ring (11) is provided with an inlet channel (111) and an overflow chamber (112). The rotating inner disc (12) is provided with a metering chamber (121) inside. The outer peripheral wall is provided with an inlet (122) and an overflow port (123) respectively communicating with the metering chamber (121). The upper and lower end faces are respectively provided with an upper connecting port (124) and a lower connecting port (125) communicating with the metering chamber (121). The rotating inner disk (12) can rotate relative to the fixed outer ring (11) so that the metering cavity (121) can switch between metering state and isolation state; an interlayer sealing part (13) is provided between adjacent rotating inner disks (12). In the initial state, the adjacent rotating inner disks (12) are misaligned in the circumferential direction so that the lower connecting port (125) of the upper rotating inner disk (12) and the upper connecting port (124) of the lower rotating inner disk (12) are in a misaligned closed state, and can rotate with the rotating inner disk (12) to the aligned connecting state; Each of the rotating inner disks (12) has an upper protrusion (126) and a lower protrusion (127) on its upper and lower end faces, respectively. The upper protrusion (126) and the lower protrusion (127) are located in the transmission area on the opposite side of the metering cavity (121). The driving member (2) is connected to the uppermost rotating inner disk (12). When the driving component (2) drives the uppermost rotating inner disk (12) to rotate, the adjacent rotating inner disks (12) follow the friction of the interlayer sealing part (13) and rotate relative to each other, so that the lower rotating inner disk (12) enters the isolation state; the upper rotating inner disk (12) continues to rotate relative to the lower rotating inner disk (12) until the lower end protrusion (127) of the upper rotating inner disk (12) abuts against the upper end protrusion (126) of the lower rotating inner disk (12), and pushes each rotating inner disk (12) from top to bottom through the adjacent protrusions, so that the adjacent connecting ports are aligned in sequence and form a linkage disk group.

2. The modular multi-component quantitative liquid preparation microfluidic chip according to claim 1, characterized in that: In the quantitative state, the liquid inlet channel (111), liquid inlet (122), quantitative chamber (121), overflow port (123) and overflow chamber (112) are connected in sequence; In the isolated state, the inlet (122) is misaligned with the inlet channel (111), and the overflow port (123) is misaligned with the overflow chamber (112), thereby isolating the metering chamber (121) from the inlet channel (111) and the overflow chamber (112).

3. The modular multi-component quantitative liquid preparation microfluidic chip according to claim 1, characterized in that: The upper and lower end faces of the fixed outer ring (11) are axially recessed relative to the corresponding end faces of the rotating inner disk (12), and the interlayer sealing part (13) is protruding on the upper and lower end faces of the rotating inner disk (12), and the interlayer sealing parts (13) of adjacent quantitative disk modules (1) are directly pressed together. The fixed outer ring (11) is arranged around the rotating inner disk (12), and the inner circumferential surface of the fixed outer ring (11) is rotatably engaged with the outer circumferential surface of the rotating inner disk (12).

4. The modular multi-component quantitative liquid preparation microfluidic chip according to claim 1, characterized in that: An inlet valve plate (4) fixed to the fixed outer ring (11) is provided between the inlet channel (111) and the inlet (122), and an overflow valve plate (5) fixed to the rotating inner disk (12) is provided between the overflow port (123) and the overflow chamber (112). The fixed ends of the inlet valve plate (4) and the overflow valve plate (5) are arranged in opposite directions along the circumference. The inlet valve (4) and the overflow valve (5) open under the action of the injection pressure and close under the action of the corresponding mating surface during the process of the rotating inner disk (12) changing from the metering state to the isolation state; the rotating inner disk (12) rotates in the same direction at a preset speed during the subsequent mixing process, so that the duration of each time the inlet (122) and the overflow (123) pass through the corresponding alignment area is less than the response time required for the corresponding valve to open from the closed state to form an effective flow gap, so that the inlet valve (4) and the overflow valve (5) remain closed during the mixing process.

5. The modular multi-component quantitative liquid preparation microfluidic chip according to claim 1, characterized in that: The upper rib (126) and the lower rib (127) are respectively long strip ribs or narrow fan-shaped ribs extending radially; The lower end protrusion (127) of the upper rotating inner disk (12) and the upper end protrusion (126) of the lower rotating inner disk (12) have an overlapping area in the axial direction, and transmit torque by abutting against the opposite sidewalls in the circumferential direction.

6. The modular multi-component quantitative liquid preparation microfluidic chip according to claim 1, characterized in that: The number of quantitative disk modules (1) and the volume of each quantitative cavity (121) are selected according to the predetermined number of components and the component ratio.

7. The modular multi-component quantitative liquid preparation microfluidic chip according to claim 1, characterized in that: It also includes a base (3) located below the lowest quantitative plate module (1), the base (3) including a fixed bottom shell (31) and a follower bottom cover (32) rotatably disposed in the fixed bottom shell (31); The following bottom cover (32) is provided with a liquid outlet (321) that can be aligned with the lower connecting port (125) of the lowest rotating inner disk (12) and a cantilever elastic plate (322) that can abut against the lower end protrusion (127) of the lowest rotating inner disk (12). The fixed bottom shell (31) is provided with a bottom cover locking member (33) for locking the following bottom cover (32). When the follower bottom cover (32) is in a free rotation state, the lower end protrusion (127) of the lowest rotating inner disk (12) drives the follower bottom cover (32) to rotate together with the linkage disk group through the cantilever elastic sheet (322), so that the lower connecting port (125) of the lowest rotating inner disk (12) and the liquid outlet (321) remain misaligned; After the follower bottom cover (32) is locked by the bottom cover locking member (33), the linkage disk assembly can continue to rotate relative to the follower bottom cover (32), so that the lower end protrusion (127) of the lowest rotating inner disk (12) pushes against and passes over the cantilever elastic plate (322) until the lower connecting port (125) is aligned with the liquid outlet (321).

8. The modular multi-component quantitative liquid preparation microfluidic chip according to claim 7, characterized in that: The cantilever elastic plate (322) includes a fixed end (3221) fixed to the follower bottom cover (32) and a free end (3222) extending toward the lowest rotating inner disk (12). The free end (3222) is provided with a guide slope (3223) that cooperates with the lower end protrusion (127).

9. The modular multi-component quantitative liquid preparation microfluidic chip according to claim 7, characterized in that: The torque required for the free rotation of the follower bottom cover (32) is less than the torque required for the cantilever elastic plate (322) to undergo over-deformation, and the locking torque provided by the bottom cover locking member (33) is greater than the torque required for the cantilever elastic plate (322) to undergo over-deformation.

10. A method for dispensing liquid using a modular multi-component quantitative liquid dispensing microfluidic chip according to any one of claims 7 to 9, wherein the modular multi-component quantitative liquid dispensing microfluidic chip further comprises the inlet valve (4) and overflow valve (5) according to claim 4, characterized in that, Includes the following steps: S1. Select multiple quantitative disk modules (1) according to the predetermined number of components and the component ratio and stack them along the axial direction so that each rotating inner disk (12) is in a quantitative state relative to the corresponding fixed outer ring (11), and make adjacent rotating inner disks (12) misaligned along the circumferential direction, with the corresponding connecting port and the corresponding protrusion in a misaligned state. S2. The corresponding components are injected through the liquid inlet channel (111) of each metering plate module (1) so that the liquid enters the metering chamber (121) and enters the overflow chamber (112) after filling the metering chamber (121). S3. Drive the uppermost rotating inner disk (12) to rotate through the driving component (2), so that the adjacent rotating inner disks (12) follow each other and rotate relative to each other through the friction of the interlayer sealing part (13), so that each rotating inner disk (12) sequentially enters the isolation state. S4. Make the lower end protrusion (127) of the upper rotating inner disk (12) abut against the upper end protrusion (126) of the lower rotating inner disk (12) in sequence, and push each rotating inner disk (12) to rotate in sequence through the adjacent protrusions, so that the adjacent connecting ports are aligned in sequence and form a linkage disk group. S5. The follower bottom cover (32) is in a free rotation state. The lower end protrusion (127) of the lowest rotating inner disk (12) drives the follower bottom cover (32) to rotate together with the linkage disk group through the cantilever elastic plate (322), so that the lower connecting port (125) of the lowest rotating inner disk (12) and the liquid outlet (321) are misaligned. The drive member (2) drives the linkage disk group to rotate continuously in the same direction at a preset speed, so that the duration of each time the liquid inlet (122) and the overflow port (123) pass through the corresponding alignment area is less than the response time required for the liquid inlet valve plate (4) and the overflow valve plate (5) to open from the closed state to form an effective flow gap, so that the liquid inlet valve plate (4) and the overflow valve plate (5) remain closed, and drive the linkage disk group to rotate a preset number of times to mix the components in each metering chamber (121). S6. After mixing is completed, the follower bottom cover (32) is locked by the bottom cover locking member (33), so that the linkage disk group continues to rotate relative to the follower bottom cover (32), so that the lower end protrusion (127) of the bottom rotating inner disk (12) pushes against and passes over the cantilever elastic plate (322) until the lower connecting port (125) of the bottom rotating inner disk (12) is aligned with the liquid outlet (321), and the mixture is discharged through the lower connecting port (125) and the liquid outlet (321).

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