A centrifugation container, a control method and a control system for the container

Abstract

The application provides a centrifugal container, a control method and a control system, relates to the technical field of tissue cell separation, and the container body is a conical revolving body, which forms an inner cavity and an outer cavity from inside to outside, is connected through a communication opening at the largest diameter, and is provided with a collection chamber; a connecting pipeline is arranged at the central axis, and the connecting pipeline comprises a liquid inlet pipeline, a liquid outlet pipeline and a flow cavity between the two; the control system comprises a driving module, a sensing module and a control part, and the liquid inlet state, the pipeline pressure, the centrifugal speed and the vibration are monitored and closed-loop controlled in real time; the control method comprises the steps of liquid inlet, centrifugal separation, waste liquid discharge, cleaning, clean liquid discharge and extraction, a resuspension action is performed in the cleaning stage, the clean liquid is spun into the outer cavity at a large speed to resuspend the matrix components, the cleaning is completed, and automatic enrichment, sufficient cleaning and full-process automatic control of the matrix components are realized.

Description

Technical Field

[0001] This invention relates to the field of tissue and cell separation technology, and in particular to a centrifuge container, a control method for the container, and a control system for the system. Background Technology

[0002] Adipose tissue transplantation is an important technique in plastic surgery and regenerative medicine, and the separation and purification of the adipose matrix components is a crucial step. Currently, centrifugation equipment is commonly used to process adipose tissue, utilizing density differences to separate different components. The separated matrix components must be washed to remove impurities before they can be used for transplantation.

[0003] However, with current traditional centrifugal separation equipment, the matrix components and waste liquid are mixed in the same chamber after centrifugation, making it difficult to achieve effective enrichment and separation extraction. Although some equipment has a separation structure, the cleaning liquid cannot directly contact the enriched matrix components during the cleaning process, resulting in insufficient cleaning and insufficient purity. At the same time, the centrifugation process relies heavily on manual operation and lacks automatic monitoring and closed-loop control of parameters such as liquid inlet status, pipeline pressure, centrifugation speed and vibration, making it difficult to guarantee processing accuracy and consistency.

[0004] Therefore, there is an urgent need for a centrifuge container that can automatically enrich and clean matrix components and has fully automated control of the entire process. Summary of the Invention

[0005] This invention provides a centrifuge container, specifically comprising: a container body, connecting pipes, and a connector. The connecting pipes are disposed at the central axis of the container body, and the end of the connecting pipes away from the container body is connected to the connector. The container body is a conical rotating body with the largest diameter at its waist and tapering at both ends. The container body forms an inner cavity and an outer cavity sequentially from the inside to the outside. The outer cavity has a collection chamber on the corresponding side of the bottom of the inner cavity. The inner cavity and the outer cavity are connected through a connecting opening located at the point of maximum diameter of the container body. The connecting pipes include an inlet pipe and an outlet pipe. The inlet pipe and the outlet pipe are a sleeve structure. The inlet pipe is in fluid communication with the inner cavity. The outlet pipe is in fluid communication with the collection chamber.

[0006] Preferably, the liquid inlet pipe is located on the outside, and the liquid outlet pipe is sleeved inside the liquid inlet pipe, forming a flow cavity between the liquid inlet pipe and the liquid outlet pipe, so that the liquid inlet pipe and the liquid outlet pipe are integrated on the same central axis, which simplifies the pipe structure and provides an independent flow space for liquid inlet.

[0007] Preferably, the flow chamber is provided with a connecting hole at the bottom, and the flow chamber is in fluid communication with the inner cavity through the connecting hole, so that the mixed liquid or cleaning liquid flows into the flow chamber through the liquid inlet pipe and is then introduced into the inner cavity through the bottom hole, forming a smooth liquid inlet channel.

[0008] Preferably, the outer cavity is provided with at least one collection channel, one end of which opens into the connecting opening and the other end opens into the collection chamber, so that the matrix components thrown out through the connecting opening are concentrated and collected into the collection chamber along the collection channel, thereby improving the enrichment efficiency of the matrix components.

[0009] Preferably, a filter assembly is provided at the connecting opening. The filter assembly is used to unidirectionally screen matrix components to prevent non-target components from entering the outer cavity and improve collection purity.

[0010] The present invention also provides a control system for a centrifuge container, for controlling the aforementioned centrifuge container, including a drive module and a sensing module; the drive module includes a centrifuge motor, an inlet pump, an outlet pump, and an extraction pump; the centrifuge motor is used to drive the container body to rotate; the inlet pump is connected to the inlet pipe and is used to input a mixed liquid or cleaning liquid into the inner cavity through the inlet pipe; the outlet pump is connected to the inlet pipe and is used to extract waste liquid or cleaning liquid from the inner cavity in reverse through the inlet pipe; the extraction pump is connected to the outlet pipe and is used to extract matrix components from the collection chamber through the outlet pipe; it also includes a control unit, which is connected to the drive module and the sensing module respectively, for controlling the drive module to execute the centrifugation process of the centrifuge container according to the feedback of the sensing module.

[0011] Preferably, the centrifugal motor adopts PID closed-loop speed regulation, with speed fluctuation less than ±1%, so that the speed is stable and controllable during the centrifugation process, ensuring the consistency of the separation effect.

[0012] Preferably, the inlet flow rate of the inlet pump is greater than the extraction flow rate of the extraction pump, so that the inlet process is completed quickly while the extraction process is carried out slowly, which improves the inlet efficiency and avoids the loss of matrix components due to being drawn away too quickly during extraction.

[0013] Preferably, the sensing module includes an optical sensor, a pressure sensor, and a speed and vibration sensor to achieve multi-dimensional monitoring of material status, pipeline pressure, and motor operating status.

[0014] Preferably, the optical sensor is installed in the liquid inlet pipe to identify the transition of the mixture to an oil state, thereby accurately determining the timing of stopping the liquid inlet and avoiding over-injection.

[0015] Preferably, the pressure sensors are respectively installed on the inlet pipe and the outlet pipe to monitor pipe blockage or rupture, and to promptly alarm and shut down the machine when the pipe is abnormal, so as to ensure safe operation.

[0016] Preferably, the speed and vibration sensors are used to monitor the speed and vibration of the centrifugal motor, and automatically stop the machine when the vibration is abnormal to prevent equipment damage.

[0017] The present invention also provides a control method for a centrifuge container, executed by the aforementioned control system, for controlling the aforementioned centrifuge container, comprising the following steps: S1 Liquid Inlet: The mixed liquid is injected into the inner cavity through the liquid inlet pipe; S2 centrifugal separation: The container body is rotated, and the mixture rises along the inner wall of the inner cavity. The matrix component with higher density enters the outer cavity through the connecting opening and is enriched in the collection chamber, while the waste liquid with lower density remains in the inner cavity. S3 Waste Liquid Discharge: After rotation stops, the waste liquid in the inner cavity settles to the bottom and is discharged in reverse through the liquid inlet pipe; S4 Cleaning: Cleaning fluid is injected into the inner cavity through the liquid inlet pipe, and a resuspension action is performed to mix the cleaning fluid with the matrix components. The container body is rotated again to re-enrich the matrix components in the collection chamber, thus completing the cleaning of the matrix components. S5 Discharge Cleaning Fluid: The cleaning fluid is discharged in reverse through the inlet pipe; S6 Extraction: The matrix components are extracted from the collection chamber through the liquid outlet pipeline.

[0018] Preferably, in step S2, after the matrix component enters the outer cavity through the connecting opening, it is collected in the collection chamber along the collection channel inside the outer cavity, so that the matrix component is concentrated and enriched, and dispersion is avoided.

[0019] Preferably, the resuspension action in S4 is as follows: a "centrifugation-stop-centrifugation" cycle is performed for a short time, and the cleaning liquid is thrown into the outer cavity at a relatively high speed, so that the cleaning liquid is mixed with the matrix components enriched in the collection chamber, and the matrix components adhering to the wall and settling to the bottom are resuspended to complete the cleaning and ensure the cleaning effect.

[0020] Preferably, in step S1, the injection is stopped when the mixture switches to an oil state, or when the injection reaches a set volume, by using the optical sensor to identify the change, thereby achieving precise control of the injection volume.

[0021] Preferably, in step S2, the container body is controlled to accelerate uniformly to a first rotational speed and maintain it for a set time, and then decelerate uniformly to zero, so that the centrifugation process is smoothly transitioned and the material disturbance is reduced.

[0022] Preferably, the method further includes: monitoring pipeline pressure using the pressure sensor in S1, S3, and S6, and alarming and shutting down when the pressure exceeds a threshold; and monitoring the rotational speed and vibration of the container body using the rotational speed and vibration sensor in S2, and automatically shutting down when the vibration exceeds a threshold, thereby achieving full-process safety monitoring.

[0023] Preferably, in step S6, the matrix components are discharged in excess into the collection container at a third flow rate lower than that injected in step S1 to avoid matrix component residue and improve the extraction rate.

[0024] Preferably, steps S4 to S5 are repeated N times, where N is a positive integer, to improve the purity of the matrix components through multiple rounds of cleaning.

[0025] Beneficial effects 1. In this invention, the container body is a conical rotating body, forming an inner cavity and an outer cavity from the inside out. The inner cavity and the outer cavity are connected by a connecting opening located at the maximum diameter of the container body. The outer cavity is provided with a collection chamber. Under the action of centrifugal force, the matrix component with a higher density automatically enters the outer cavity through the connecting opening and is collected in the collection chamber along the collection channel, thereby achieving effective separation and automatic enrichment of the matrix component and the waste liquid. The liquid inlet pipe and the liquid outlet pipe adopt a sleeve structure to form a flow cavity. The bottom of the flow cavity is provided with a connecting hole that connects to the inner cavity, so that the liquid is injected into the inner cavity through the central axis to form a smooth liquid inlet flow channel, which simplifies the pipe structure and realizes the integration of liquid inlet and liquid outlet. 2. In this invention, optical sensors are used to accurately determine when to stop the liquid inlet, pressure sensors are used to monitor pipe blockage or rupture, and speed and vibration sensors are used to monitor the speed fluctuation and dynamic balance of the centrifugal motor, thus achieving full-process multi-dimensional automated safety monitoring. The control method performs a resuspension action during the cleaning stage. Through a short "centrifugation-stop-centrifugation" cycle and a large speed, the cleaning liquid is thrown into the outer cavity, so that the cleaning liquid mixes with the matrix components enriched in the collection chamber, and the matrix components attached to the wall and settled at the bottom are resuspended to complete the cleaning, which effectively improves the cleaning effect and matrix purity. Attached Figure Description

[0026] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings of the embodiments will be briefly described below.

[0027] The accompanying drawings described below are only related to some embodiments of the invention and are not intended to limit the invention.

[0028] In the attached diagram: Figure 1 An overall isometric view of a centrifuge container according to an embodiment of the present invention is shown.

[0029] Figure 2 A cross-sectional view of a centrifuge container according to an embodiment of the present invention is shown.

[0030] Figure 3 A complete cross-sectional axonometric view of a centrifuge container according to an embodiment of the present invention is shown.

[0031] Figure 4 A centrifuge container according to an embodiment of the present invention is shown. Figure 3A magnified view of a portion of point A shown.

[0032] Figure 5 A centrifuge container according to an embodiment of the present invention is shown. Figure 3 A magnified view of a portion of point B shown.

[0033] Figure 6 An assembly diagram of a centrifuge container according to an embodiment of the present invention is shown.

[0034] Figure 7 A flowchart of a control method for a centrifuge container according to an embodiment of the present invention is shown.

[0035] Figure 8 A connection diagram of the control system of a centrifuge container according to an embodiment of the present invention is shown.

[0036] List of main reference numerals 1. Container body; 101. Inner cavity; 102. Outer cavity; 1021. Connecting opening; 1022. Collection channel; 103. Collection chamber; 2. Connecting pipes; 201. Liquid inlet pipe; 2011. Connecting through hole; 202. Liquid outlet pipe; 203. Flow chamber; 3. Connector; 4. Drive module; 401. Centrifugal motor; 402. Inlet pump; 403. Outlet pump; 404. Extraction pump; 5. Sensing module; 501. Optical sensor; 502. Pressure sensor; 503. Speed ​​and vibration sensor. Detailed Implementation

[0037] To make the objectives, technical solutions, and advantages of this application clearer, the technical solutions of this application will be clearly and completely described below in conjunction with specific embodiments and corresponding drawings. Obviously, the described embodiments are only a part of the embodiments of this application, and not all of them. Based on the embodiments in this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0038] In this document, “illustrative” means “serving as an example, illustration or description”, and any illustration or implementation described herein as “illustrative” should not be construed as a more preferred or advantageous technical solution.

[0039] To keep the drawings concise, only the parts relevant to this application are shown schematically in each drawing, and they do not represent the actual structure of the product. In addition, to make the drawings concise and easy to understand, in some drawings, only one of the components with the same structure or function is shown schematically, or only one of them is labeled.

[0040] In this document, unless otherwise expressly specified and limited, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance; unless otherwise specified or explained, the term "multiple" refers to two or more; the terms "connected," "fixed," etc., should be interpreted broadly. For example, "connected" can be a fixed connection, a detachable connection, an integral connection, or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this application according to the specific circumstances.

[0041] Example: Please refer to Figures 1 to 8 : This invention proposes a centrifuge container, comprising: a container body 1, a connecting pipe 2, and a connector 3; wherein, the container body 1 is a conical rotating body with the largest diameter at its waist and tapering at both ends; the connecting pipe 2 is provided at the central axis of the container body 1, and the end of the connecting pipe 2 away from the container body 1 is connected to the connector 3; the connector 3 is a universal connecting component, which has been separately patented, and its specific structure will not be described in detail in this case.

[0042] like Figure 1 , Figure 2 and Figure 3 As shown, in this embodiment, the container body 1 is a conical rotating body with the largest diameter at its waist and tapering at both ends. The container body 1 forms an inner cavity 101 and an outer cavity 102 from the inside out. The outer cavity 102 has a collection chamber 103 on the side corresponding to the bottom of the inner cavity 101. The inner cavity 101 and the outer cavity 102 are connected by a connecting opening 1021 located at the largest diameter of the container body 1. The outer cavity 102 has at least one collection channel 1022, with one end of the collection channel 1022 opening into the connecting opening 1021 and the other end opening into the collection chamber 103. A filter assembly, which is a filter membrane, can be provided at the connecting opening 1021 for unidirectional screening of matrix components. The rotation of the container body 1 generates centrifugal force. Under the action of centrifugal force, the matrix components with higher density in the mixture are thrown towards the largest diameter of the container body 1, enter the outer cavity 102 through the connecting opening 1021, and collect in the collection chamber 103 along the collection channel 1022 for enrichment. The waste liquid with lower density is retained in the inner layer of the inner cavity 101 near the central axis.

[0043] like Figure 1 , Figure 3 and Figure 4As shown, in this embodiment, the connecting pipe 2 is located at the central axis of the container body 1, and includes an inlet pipe 201 and an outlet pipe 202. The inlet pipe 201 is located on the outside, and the outlet pipe 202 is sleeved inside the inlet pipe 201, forming a flow cavity 203 between the inlet pipe 201 and the outlet pipe 202. The bottom of the flow cavity 203 is provided with a connecting hole 2011, and the flow cavity 203 is in fluid communication with the inner cavity 101 through the connecting hole 2011. The inlet pipe 201 is in fluid communication with the inner cavity 101 and is used to inject the mixed liquid or cleaning liquid into the inner cavity 101 and to extract waste liquid or cleaning liquid from the inner cavity 101 in the reverse direction. The outlet pipe 202 is in fluid communication with the collection chamber 103 and is used to extract the matrix component from the collection chamber 103. A sealing section is provided between the inlet pipe 201 and the outlet pipe 202, and the sealing section is used to isolate the inlet pipe 201 and the outlet pipe 202 to prevent crossflow.

[0044] like Figure 6 As shown, in this embodiment, the connector 3 is connected to the end of the connecting pipe 2 away from the container body 1. It is a universal connecting component that integrates the container rotation and liquid inlet / outlet functions.

[0045] The present invention also proposes a control system for a centrifuge container, which is used to control the centrifuge container, including: a drive module 4, a sensing module 5 and a control unit. The control unit is connected to the drive module 4 and the sensing module 5 respectively, and is used to control the drive module 4 to execute the centrifugation process of the centrifuge container according to the feedback of the sensing module 5.

[0046] like Figure 8 As shown, in this embodiment, the drive module 4 includes a centrifugal motor 401, an inlet pump 402, an outlet pump 403, an extraction pump 404, and valves. The centrifugal motor 401 drives the container body 1 to rotate, employing PID closed-loop speed regulation with a speed fluctuation of less than ±1%. The inlet pump 402 is connected to the inlet pipe 201 and is used to input the mixed liquid or cleaning liquid into the inner cavity 101 via the inlet pipe 201. The inlet flow rate of the inlet pump 402 is greater than the extraction flow rate of the extraction pump 404. The discharge pump 403 is connected to the inlet pipe 201 and is used to extract waste liquid or cleaning liquid from the inner cavity 101 in reverse through the inlet pipe 201; the extraction pump 404 is connected to the outlet pipe 202 and is used to extract matrix components from the collection chamber 103 through the outlet pipe 202; the valves include an inlet channel valve, a drain channel valve and an extraction channel valve, which are respectively installed on the corresponding pipes of the inlet pipe 201 and the outlet pipe 202, and are controlled by the control unit to switch the on / off state of each channel.

[0047] like Figure 8As shown, in this embodiment, the sensing module 5 includes an optical sensor 501, a pressure sensor 502, and a speed and vibration sensor 503. The optical sensor 501 is installed in the liquid inlet pipe 201 to identify the change in the liquid state from a mixture state to an oil state in the liquid inlet pipe 201, so as to determine that the mixture delivery is complete. Since the liquid state needs to be detected in real time during the liquid inlet, the pumping speed of the liquid inlet pump 402 cannot be too fast—too fast will cause the speed of the fluid flowing through the sensor to exceed the response time of the system signal processing and valve mechanical action, resulting in an uncontrollable volume of excess oil being inertially pushed into the inner cavity 101 before the system completely closes the liquid inlet channel valve, thereby destroying the extraction purity. It also cannot be too slow—too slow will... To increase processing time, the inlet pump 402 is set at a medium speed, between too fast and too slow. Pressure sensors 502 are installed on the corresponding pipes of the inlet pipe 201 and the outlet pipe 202 to monitor the rotary seal structure or pipe blockage or rupture. When the inlet pressure exceeds the threshold (e.g., 110% of the reference value and held for 1 second), it is determined that the rotary seal structure or pipe is blocked, triggering an alarm and stopping the machine. Speed ​​and vibration sensors 503 are used to monitor the speed and vibration of the centrifugal motor 401. The speed monitoring uses closed-loop PID control to ensure that the speed fluctuation is less than ±1%. Vibration monitoring is used to monitor the dynamic balance of the centrifugal motor 401. When the amplitude exceeds the limit (e.g., 110% of the reference value), the system automatically stops.

[0048] like Figure 8 As shown, in this embodiment, the control unit is connected to the drive module 4 and the sensing module 5 respectively, and is used to control the drive module 4 to execute the centrifugation process of the centrifuge container according to the feedback of the sensing module 5. Specifically, the control unit controls the start and stop of the liquid inlet pump 402 and the opening and closing of the liquid inlet channel valve according to the state switching signal of the optical sensor 501 or the liquid inlet volume setting threshold, controls the opening and closing of each channel valve according to the pressure signal of the pressure sensor 502 and alarms and stops the machine when the threshold is exceeded, and adjusts the speed of the centrifuge motor 401 according to the closed loop of the speed and vibration sensor 503 and automatically stops the machine when the vibration exceeds the limit.

[0049] The present invention also proposes a control method for a centrifuge container, which is executed by the aforementioned control system to control the centrifuge container.

[0050] like Figure 7 As shown, in this embodiment, the control method includes the following steps: S1 Liquid Injection: The control unit opens the liquid inlet channel valve and starts the liquid inlet pump 402 at a medium speed (e.g., 200 ml / min), injecting the mixed liquid into the inner cavity 101 through the liquid inlet pipe 201, and recording the running time of the liquid inlet pump 402. To calculate the influent volume (= medium speed × When the optical sensor 501 detects that the liquid state in the liquid inlet pipe 201 has switched from a mixture state to an oil state, it determines that the mixture delivery is complete, controls the liquid inlet pump 402 to stop and closes the liquid inlet channel valve; or when the liquid inlet volume... Reaching the set threshold When the volume is 300ml, the inlet pump 402 is stopped and the inlet channel valve is closed. The pump speed of the inlet pump 402 is selected as medium speed to balance detection accuracy and processing efficiency. If the pump speed is too fast, the speed at which the fluid flows through the sensor will exceed the response time of the system signal processing and valve mechanical action. This will cause an uncontrollable volume of excess oil to be pushed into the inner cavity 101 by inertia before the inlet channel valve is completely closed, thus destroying the extraction purity. If the pump speed is too slow, the processing time will be increased. The inlet pressure is monitored in real time by the pressure sensor 502. When the inlet pressure exceeds the threshold (e.g., 110% of the reference value and held for 1 second), it is determined that the rotary seal structure or pipeline is blocked, triggering an alarm and stopping the machine. S2 Centrifugal Separation: The control unit controls the centrifugal motor 401 to accelerate to the first speed (separation setting, e.g., 3000 rpm) at a set acceleration (e.g., uniformly accelerating from 0 to 3000 rpm within 30 seconds), and maintains the first speed for a set separation time (e.g., 10 minutes). After the separation time ends, the control unit controls the centrifugal motor 401 to decelerate to zero at a set acceleration (e.g., uniformly decelerating from 3000 rpm to 0 within 30 seconds). Under continuous centrifugal force, based on density differences, the densest fatty matrix component is thrown towards the two sides of the container body 1 where the diameter is largest. After entering the outer cavity 102 through the connecting opening 1021, the waste liquid is collected in the collection chamber 103 along the collection channel 1022 and enriched. The waste liquid with lower density (free lipid droplets, water, enzymes, etc.) is suspended and retained in the inner layer of the inner cavity 101 near the central axis. During the deceleration process, the lipid matrix components fall due to gravity and gather in the collection chamber 103 on the corresponding side of the bottom of the outer cavity 102. The closed-loop PID control of the speed and vibration sensor 503 ensures that the speed fluctuation is less than ±1% and monitors the dynamic balance of the centrifugal motor 401. When the amplitude exceeds the limit (e.g., 110% of the reference value), the system automatically stops. S3 Waste Liquid Discharge: After rotation stops, the remaining waste liquid in the inner cavity 101 settles to the bottom. The control unit opens the drain channel valve and starts the outlet pump 403 to draw the waste liquid in reverse at high speed (e.g., 300 ml / min) and discharge it through the inlet pipe 201. The discharge time is as follows: or — (High-speed) calculation; monitors pipeline pressure via pressure sensor 502, and alarms and shuts down when the pressure exceeds the threshold; S4 Cleaning: The control unit opens the inlet channel valve and starts the inlet pump 402 to inject cleaning fluid into the inner cavity 101 at high speed (e.g., 300 ml / min) through the inlet pipe 201. Pump running time Calculated based on the maximum amount as [( - [High speed]; then perform the resuspension action—perform a "centrifugation-stop-centrifugation" cycle in a short time, for example, accelerate from 0 to 1200 rpm within 2 seconds, then decelerate from 1200 rpm to 0 within 2 seconds, repeat 15 times, and throw the cleaning fluid into the outer cavity 102 at a higher speed, so that the cleaning fluid mixes with the matrix components enriched in the collection chamber 103, and the matrix components adhering to the wall and settling to the bottom are resuspended to complete the cleaning; since the cleaning fluid just added in scheme 2 does not directly contact the fatty matrix components in the collection chamber 103, A high-speed resuspension action is required to throw the cleaning fluid into the outer cavity 102 and mix it with the fatty matrix components. After the resuspension action is completed, the centrifuge motor 401 is controlled to accelerate to the first speed at a set acceleration (e.g., from 0 to 3000 rpm within 30 seconds) and maintain it for a set separation time (e.g., 10 minutes). Then, it is decelerated to zero, so that the cleaned matrix components re-enter the outer cavity 102 through the connecting opening 1021 and are collected in the collection chamber 103 for enrichment. S4 can be repeated N times, where N is a positive integer (e.g., 3 times). S5 Discharge Cleaning Fluid: The cleaning fluid is discharged in reverse through the inlet pipe 201. The action sequence is the same as that of S3 Discharge Waste Liquid—open the discharge channel valve, start the outlet pump 403 to draw the cleaning fluid in reverse at high speed and discharge it through the inlet pipe 201; monitor the pipeline pressure through the pressure sensor 502, and alarm and stop when the pressure exceeds the threshold. S6 Extraction: The control unit opens the extraction channel valve and starts the extraction pump 404 at an ultra-low speed (e.g., 30 ml / min) to extract the matrix components from the collection chamber 103 through the outlet pipeline 202 and transport them to the collection container. Pump running time It can be set to over-discharge (e.g., =150% × ( ( / ultra-low speed) to avoid fatty matrix components remaining in the container body 1; the pressure sensor 502 monitors the pipeline pressure, and an alarm will be triggered and the machine will stop when the pressure exceeds the threshold.

[0051] The specific usage and function of this embodiment: The control unit opens the inlet channel valve and starts the inlet pump 402 to inject the mixture into the inner cavity 101 at a medium speed through the inlet pipe 201. The injection stops when the optical sensor 501 detects that the liquid state has switched from a mixture state to an oil state, or when the liquid has been injected to a set volume. The centrifugal motor 401 is controlled to accelerate uniformly to the first rotational speed at a set acceleration and maintain the set separation time. The mixture rises along the inner wall of the inner cavity 101. The densest fatty matrix component is thrown towards the largest diameter of the container body 1 and enters the outer cavity 102 through the connecting opening 1021. It is collected in the collection chamber 103 along the collection channel 1022 and enriched. The less dense waste liquid is suspended and retained in the inner layer of the inner cavity 101. During the deceleration process, the fatty matrix component falls due to gravity and gathers in the collection chamber 103. After the rotation stops, the drain channel valve is opened and the outlet pump 403 is started to draw the waste liquid in the inner cavity 101 at high speed and discharge it through the inlet pipe 201. The inlet channel valve is opened and the inlet pump 402 is started to inject the cleaning liquid at high speed to perform the resuspension action. —The process involves a short cycle of centrifugation-stop-centrifugation, with the cleaning solution being thrown into the outer chamber 102 at a high speed to mix with the matrix components. This resuspends the matrix components that are attached to the walls and settled at the bottom, completing the cleaning process. Centrifugation is then performed again to collect the cleaned matrix components in the collection chamber 103. This process can be repeated N times from S4 to S5. After cleaning, the cleaning solution is discharged in reverse at high speed through the inlet pipe 201. Finally, the extraction channel valve is opened, and the extraction pump 404 is started to extract the excess matrix components from the collection chamber 103 through the outlet pipe 202 at an ultra-low speed, which are then discharged into the collection container. Throughout the process, the pressure sensor 502 monitors the pipeline pressure and alarms to stop the machine when it is abnormal. The speed and vibration sensor 503 monitors the speed fluctuations and dynamic balance abnormalities and automatically stops the machine, thus achieving safety monitoring.

[0052] The above description is merely a specific embodiment of this application. Under the teachings of this application, those skilled in the art can make other improvements or modifications based on the above embodiments. Those skilled in the art should understand that the above specific description is only to better explain the purpose of this application, and the scope of protection of this application should be determined by the scope of the claims.

Claims

1. A centrifuge container, comprising a container body (1), a connecting pipe (2), and a connector (3), wherein the connecting pipe (2) is disposed at the central axis of the container body (1), and the end of the connecting pipe (2) away from the container body (1) is connected to the connector (3), characterized in that: The container body (1) is a conical rotating body with the largest diameter at its waist and tapering at both ends; the container body (1) forms an inner cavity (101) and an outer cavity (102) from the inside to the outside; the outer cavity (102) has a collection chamber (103) on the side corresponding to the bottom of the inner cavity (101); the inner cavity (101) and the outer cavity (102) are connected by a connecting opening (1021) located at the largest diameter of the container body (1); The connecting pipeline (2) includes an inlet pipeline (201) and an outlet pipeline (202); the inlet pipeline (201) and the outlet pipeline (202) are sleeve structures; the inlet pipeline (201) is in fluid communication with the inner cavity (101); the outlet pipeline (202) is in fluid communication with the collection chamber (103).

2. A centrifuge container according to claim 1, characterized in that, The liquid inlet pipe (201) is located on the outside, and the liquid outlet pipe (202) is sleeved inside the liquid inlet pipe (201). A flow cavity (203) is formed between the liquid inlet pipe (201) and the liquid outlet pipe (202).

3. A centrifuge container according to claim 2, characterized in that, The bottom of the flow cavity (203) is provided with a through hole (2011), and the flow cavity (203) is in fluid communication with the inner cavity (101) through the through hole (2011).

4. A centrifuge container according to claim 1, characterized in that, The outer cavity (102) is provided with at least one collection channel (1022), one end of which opens into the connecting opening (1021) and the other end opens into the collection chamber (103).

5. A centrifuge container according to claim 1, characterized in that, A filter assembly is provided at the connecting opening (1021), which is used for unidirectional screening of matrix components.

6. A control system for a centrifuge container, used to control the centrifuge container as described in any one of claims 1-5, characterized in that, The system includes a drive module (4) and a sensing module (5). The drive module (4) includes a centrifugal motor (401), an inlet pump (402), an outlet pump (403), and an extraction pump (404). The centrifugal motor (401) is used to drive the container body (1) to rotate. The inlet pump (402) is connected to the inlet pipe (201) and is used to input a mixed liquid or cleaning liquid into the inner cavity (101) through the inlet pipe (201). The outlet pump (403) is connected to the inlet pipe (201) and is used to extract waste liquid or cleaning liquid from the inner cavity (101) in reverse through the inlet pipe (201). The extraction pump (404) is connected to the outlet pipe (202) and is used to extract matrix components from the collection chamber (103) through the outlet pipe (202). It also includes a control unit, which is connected to the drive module (4) and the sensing module (5) respectively, and is used to control the drive module (4) to execute the centrifugation process of the centrifuge container according to the feedback of the sensing module (5).

7. The control system for a centrifuge container according to claim 6, characterized in that, The centrifugal motor (401) adopts PID closed-loop speed regulation, and the speed fluctuation is less than ±1%.

8. The control system for a centrifuge container according to claim 6, characterized in that, The inlet flow rate of the inlet pump (402) is greater than the extraction flow rate of the extraction pump (404).

9. The control system for a centrifuge container according to claim 6, characterized in that, The sensing module (5) includes an optical sensor (501), a pressure sensor (502), and a speed and vibration sensor (503).

10. The control system for a centrifuge container according to claim 9, characterized in that, The optical sensor (501) is disposed in the liquid inlet pipe (201) and is used to identify the switching of the mixture to the oil state.

11. The control system for a centrifuge container according to claim 9, characterized in that, The pressure sensors (502) are respectively installed on the pipelines corresponding to the liquid inlet pipeline (201) and the liquid outlet pipeline (202) to monitor pipeline blockage or rupture.

12. The control system for a centrifuge container according to claim 9, characterized in that, The speed and vibration sensor (503) is used to monitor the speed and vibration of the centrifugal motor (401).

13. A method for controlling a centrifuge container, executed by a control system as described in claims 6-12, for controlling a centrifuge container as described in any one of claims 1-5, characterized in that, Includes the following steps: S1 Liquid Inlet: The mixture is injected into the inner cavity (101) through the liquid inlet pipe (201); S2 Centrifugal separation: The container body (1) is rotated, and the mixture rises along the inner wall of the inner cavity (101). The matrix component with higher density enters the outer cavity (102) through the connecting opening (1021) and is enriched in the collection chamber (103), while the waste liquid with lower density remains in the inner cavity (101). S3 Waste liquid discharge: After the rotation stops, the waste liquid in the inner cavity (101) settles to the bottom and is discharged in the reverse direction through the liquid inlet pipe (201); S4 Cleaning: Cleaning fluid is injected into the inner cavity (101) through the liquid inlet pipe (201), and a resuspension action is performed to mix the cleaning fluid with the matrix components. The container body (1) is rotated again to re-enrich the matrix components in the collection chamber (103), thus completing the cleaning of the matrix components. S5 Discharge cleaning fluid: The cleaning fluid is discharged in reverse through the inlet pipe (201); S6 Extraction: The matrix components are extracted from the collection chamber (103) through the liquid outlet pipe (202).

14. The control method for a centrifuge container according to claim 13, characterized in that, In S2, the matrix component enters the outer cavity (102) through the connecting opening (1021) and then gathers into the collection chamber (103) along the collection channel (1022) in the outer cavity (102).

15. The control method for a centrifuge container according to claim 13, characterized in that, The resuspension action in S4 is as follows: a "centrifugation-stop-centrifugation" cycle is performed in a short time, and the cleaning liquid is thrown into the outer cavity (102) at a large speed, so that the cleaning liquid is mixed with the matrix components enriched in the collection chamber (103), and the matrix components attached to the wall and settled at the bottom are resuspended to complete the cleaning.

16. The control method for a centrifuge container according to claim 13, characterized in that, In step S1, the injection stops when the mixture is switched to an oil state by the optical sensor (501), or when the mixture is injected to a set volume.

17. The control method for a centrifuge container according to claim 13, characterized in that, In step S2, the container body (1) is controlled to accelerate uniformly to a first rotational speed and maintain it for a set duration, and then decelerate uniformly to zero.

18. A control method for a centrifuge container according to claim 13, characterized in that, Also includes: In S1, S3, and S6, the pressure in the pipeline is monitored by the pressure sensor (502), and an alarm is triggered and the machine is shut down when the pressure exceeds the threshold. In S2, the rotation speed and vibration of the container body (1) are monitored by the rotation speed and vibration sensor (503), and the machine is automatically shut down when the vibration exceeds the threshold.

19. A control method for a centrifuge container according to claim 13, characterized in that, In step S6, the matrix components are discharged in excess into the collection container at a third flow rate lower than that injected in step S1.

20. A control method for a centrifuge container according to claim 13, characterized in that, S4 to S5 are executed N times, where N is a positive integer.

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