Liver disease blood sample storage system, control method, control device, and storage medium

Abstract

This invention discloses a liver disease blood sample storage system, control method, equipment, and storage medium. The liver disease blood sample storage system includes: a cabinet with an openable and closable door, the cabinet and door together enclosing an internal storage space; a basic cryopreservation box and multiple extended cryopreservation boxes disposed within the internal storage space; the extended cryopreservation boxes being stacked and connected below or above the basic cryopreservation box; a three-dimensional dynamic mixing module disposed within the storage cavities of the basic and extended cryopreservation boxes, performing mixing operations according to vibration intensity and time; a temperature and humidity sensor group disposed within the walls of the basic and extended cryopreservation boxes, detecting temperature and humidity data within the storage space; a controller electrically connected to the three-dimensional dynamic mixing module, and electrically connected to the temperature and humidity sensor group through an electrical contact point group, predicting temperature change trends based on temperature and humidity data and adjusting the temperature of the storage space accordingly. This invention can predict internal temperature changes and adjust the internal temperature in real time, improving the accuracy of storage temperature control.

Description

Technical Field

[0001] This invention relates to the technical field of blood sample storage for liver diseases, and in particular to a blood sample storage system, control method, control equipment, and storage medium for liver diseases. Background Technology

[0002] Blood samples from liver disease patients are typically stored using low-temperature freezing or refrigeration. The collected blood samples are placed in storage containers or cryopreservation boxes to maintain the biological activity of viral nucleic acids (such as HBV DNA and HCV RNA) and antibodies in the blood samples for subsequent clinical testing or research use.

[0003] During blood sample preservation, the temperature control inside the storage device relies on the operator's experience and judgment. The temperature control method is mostly passive real-time monitoring and feedback adjustment, which lacks the ability to predict future temperature changes and makes it difficult to deal with sudden temperature changes and other emergencies in a timely manner, resulting in low accuracy of storage device temperature control. Summary of the Invention

[0004] This invention aims to at least solve one of the technical problems existing in the prior art. To this end, this invention proposes a liver disease blood sample storage system that can predict changes in internal temperature and adjust the internal temperature in real time, thereby improving the accuracy of temperature control in the storage device.

[0005] This invention also proposes a control method for a liver disease blood sample storage system.

[0006] The present invention also proposes a control device for a liver disease blood sample storage system.

[0007] The present invention also proposes a computer-readable storage medium.

[0008] In a first aspect, one embodiment of the present invention provides a liver disease blood sample storage system, comprising:

[0009] The box body is equipped with an openable and closable door, and the box body and the door together enclose an internal storage space;

[0010] A basic cryopreservation box and multiple extended cryopreservation boxes are disposed within the internal storage space. The extended cryopreservation boxes are detachably stacked and connected to the basic cryopreservation box below or above it via a snap-fit ​​mechanism.

[0011] A three-dimensional dynamic mixing module is installed in the storage cavity within the basic cryopreservation box and the extended cryopreservation box, and is used to perform mixing operations according to preset vibration intensity and time.

[0012] A temperature and humidity sensor array is installed in the walls of the basic cryopreservation box and the extended cryopreservation box to detect temperature and humidity data within the storage space.

[0013] The controller is located inside the basic cryogenic storage box and is electrically connected to the control terminal of the three-dimensional dynamic mixing module. It is also electrically connected to the temperature and humidity sensor group through an electrical contact point group. The controller is used to predict the temperature change trend based on the temperature and humidity data and adjust the temperature of the storage space.

[0014] The liver disease blood sample storage system of this invention has at least the following beneficial effects: When it is necessary to increase the storage capacity, the operator only needs to connect the new extended cryopreservation box to the bottom or top of the existing basic cryopreservation box and / or other extended cryopreservation boxes via a snap-fit ​​mechanism; when it is necessary to reduce the storage capacity, the operator only needs to release the snap-fit ​​mechanism to remove the extended cryopreservation box. A three-dimensional dynamic mixing module is used to perform a mixing operation on the sample tubes placed in the storage chamber according to a preset vibration intensity and time to prevent component precipitation and stratification of the blood sample during long-term storage. Temperature and humidity sensor groups are embedded in the walls of both the basic cryopreservation box and the extended cryopreservation box. The sensors are distributed in different areas of the walls of each cryopreservation box to achieve multi-point monitoring of temperature and humidity data at different locations within the internal storage space. Electrical contact point groups are correspondingly provided at the bottom and top of the basic cryopreservation box and the bottom and top of the extended cryopreservation box. When the extended cryopreservation box is stacked and connected to the basic cryopreservation box via a snap-fit ​​mechanism, the elastic pins and contact points contact each other to achieve power supply and signal transmission. During operation, the controller first acquires real-time temperature and humidity data from the temperature and humidity sensor group via electrical connection contact points and constructs a historical temperature and humidity sequence. Subsequently, the controller inputs this historical sequence into a time series prediction model, which then predicts the temperature and humidity trends over a preset time period. Finally, based on the predicted temperature and humidity trends, the controller adjusts the cooling power of the refrigeration unit or activates the heating unit in advance, ensuring that the temperature of the internal storage space remains within the preset target temperature range. This proactive intervention before temperature anomalies occur effectively prevents the degradation of viral nucleic acids (such as HBV DNA and HCV RNA) or antibody inactivation in blood samples due to sudden temperature changes, significantly improving the stability and reliability of liver disease blood sample storage.

[0015] According to other embodiments of the liver disease blood sample storage system of the present invention, the liver disease blood sample storage system further includes a multi-level threshold comparator disposed in the basic cryopreservation box and electrically connected to the controller, for comparing the temperature and humidity data with preset warning thresholds and triggering an emergency response of the corresponding level.

[0016] According to other embodiments of the liver disease blood sample storage system of the present invention, the liver disease blood sample storage system further includes: an RFID reader / writer disposed on the basic cryopreservation box and the extended cryopreservation box, electrically connected to the controller through the electrical contact point group, for scanning the RFID tag on the sample storage tube to obtain the basic information of the sample storage tube; wherein, the basic information includes: location information, storage duration and sample type.

[0017] According to other embodiments of the liver disease blood sample storage system of the present invention, the three-dimensional dynamic mixing module includes a rocking oscillation structure, a lifting oscillation structure, and a left-right swinging oscillation structure stacked sequentially from bottom to top. A blood vessel placement seat that can slide relative to each other is provided on the left-right swinging oscillation structure. The rocking oscillation structure is used to drive the lifting oscillation structure, the left-right swinging oscillation structure, and the blood vessel placement seat to reciprocate along a first direction. The lifting oscillation structure is used to drive the left-right swinging oscillation structure and the blood vessel placement seat to reciprocate up and down along a vertical direction. The left-right swinging oscillation structure is used to drive the blood vessel placement seat to slide back and forth along a second direction. The first direction, the vertical direction, and the second direction constitute three mutually orthogonal directions in a three-dimensional spatial coordinate system.

[0018] In a second aspect, one embodiment of the present invention provides a control method for a liver disease blood sample storage system, applied to the liver disease blood sample storage system described in the first aspect, the control method comprising:

[0019] Collect temperature and humidity data and construct historical temperature and humidity sequences;

[0020] The historical temperature and humidity series is input into the time series prediction model to predict the temperature and humidity change trend within a preset time period.

[0021] Adjust the cooling or heating power according to the temperature and humidity change trend.

[0022] According to other embodiments of the present invention, the control method of a liver disease blood sample storage system, wherein adjusting the cooling power or heating power according to the temperature and humidity change trend includes:

[0023] When the temperature and humidity change trend indicates that it will exceed the first temperature threshold within a preset time period, the cooling power will be started in advance or increased.

[0024] When the temperature and humidity change trend indicates that the temperature is below the second temperature threshold within the preset time period, the cooling power is reduced or the heating device is activated; wherein, the first temperature threshold is greater than the second temperature threshold.

[0025] The control method for a liver disease blood sample storage system according to other embodiments of the present invention further includes:

[0026] The three-dimensional dynamic mixing cycle is determined based on the storage time of the sample tube and the sample type.

[0027] The quality change trend of the sample tube under different storage conditions is predicted by simulation algorithm;

[0028] The three-dimensional dynamic mixing cycle is dynamically optimized based on the quality change trend, and a mixing operation is performed when the three-dimensional dynamic mixing cycle is reached.

[0029] According to other embodiments of the present invention, a control method for a liver disease blood sample storage system, wherein determining the three-dimensional dynamic mixing cycle based on the storage duration and sample type of the sample storage tube includes:

[0030] For blood samples whose storage time is less than a first preset time, the three-dimensional dynamic mixing cycle is set as the first cycle.

[0031] For blood samples with a storage duration greater than or equal to the first preset duration, the three-dimensional dynamic mixing cycle is shortened to a second cycle; wherein the second cycle is shorter than the first cycle;

[0032] When the coagulation risk of the sample type is higher than the preset risk level, the three-dimensional dynamic mixing cycle is set to the third cycle; wherein the third cycle is shorter than the second cycle.

[0033] Thirdly, one embodiment of the present invention provides a control device for a liver disease blood sample storage system, comprising:

[0034] At least one processor, and,

[0035] A memory communicatively connected to the at least one processor; wherein,

[0036] The memory stores instructions that can be executed by the at least one processor to enable the at least one processor to perform the control method for the liver disease blood sample storage system as described in the second aspect.

[0037] Fourthly, one embodiment of the present invention provides a computer-readable storage medium storing computer-executable instructions for causing a computer to perform a control method for a liver disease blood sample storage system as described in the second aspect.

[0038] Other features and advantages of this application will be set forth in the following description and will be apparent in part from the description or may be learned by practicing the application. The objectives and other advantages of this application may be realized and obtained by means of the structures particularly pointed out in the description and the accompanying drawings. Attached Figure Description

[0039] Figure 1 This is a schematic diagram of a specific embodiment of the liver disease blood sample storage system in this invention;

[0040] Figure 2 This is a schematic flowchart of a specific embodiment of the control method in this invention;

[0041] Figure 3 yes Figure 2 A schematic diagram of a specific embodiment of step 203;

[0042] Figure 4 This is a schematic flowchart of a specific embodiment of the control method in this invention;

[0043] Figure 5 yes Figure 4 A schematic flowchart of a specific embodiment of step 401.

[0044] Explanation of reference numerals in the attached figures:

[0045] Box 1, Box door 2, Basic cryopreservation box 3, Extended cryopreservation box 4. Detailed Implementation

[0046] The following will describe the concept and technical effects of the present invention clearly and completely with reference to embodiments, so as to fully understand the purpose, features and effects of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are all within the scope of protection of the present invention.

[0047] In the description of this invention, if directional descriptions are involved, such as "up," "down," "front," "back," "left," "right," etc., indicating the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, it is only for the convenience of describing the invention and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the invention. If a feature is referred to as "set," "fixed," "connected," or "installed" on another feature, it can be directly set, fixed, or connected to the other feature, or it can be indirectly set, fixed, connected, or installed on the other feature.

[0048] In the description of the embodiments of the present invention, the term "several" means one or more, and the term "multiple" means two or more. The terms "greater than," "less than," and "exceeding" should be understood as excluding the stated number, while the terms "above," "below," and "within" should be understood as including the stated number. The terms "first" and "second" should be understood as distinguishing technical features, and not as indicating or implying relative importance, the number of indicated technical features, or the order of the indicated technical features.

[0049] Blood samples from liver disease patients are typically stored using low-temperature freezing or refrigeration. The collected blood samples are placed in storage containers or cryopreservation boxes to maintain the biological activity of viral nucleic acids (such as HBV DNA and HCV RNA) and antibodies in the blood samples for subsequent clinical testing or research use.

[0050] During blood sample preservation, the temperature control inside the storage device relies on the operator's experience and judgment. The temperature control method is mostly passive real-time monitoring and feedback adjustment, which lacks the ability to predict future temperature changes and makes it difficult to deal with sudden temperature changes and other emergencies in a timely manner, resulting in low accuracy of storage device temperature control.

[0051] This invention aims to at least solve one of the technical problems existing in the prior art. To this end, this invention proposes a liver disease blood sample storage system that can predict changes in internal temperature and adjust the internal temperature in real time, thereby improving the accuracy of temperature control in the storage device.

[0052] This application discloses a blood sample storage system for liver disease. Please refer to the embodiments below. Figure 1 , Figure 1 This is a schematic diagram of a liver disease blood sample storage system according to an embodiment of the present invention. In some embodiments, the liver disease blood sample storage system includes: a housing 1, a basic cryopreservation box 3, multiple extended cryopreservation boxes 4, a three-dimensional dynamic mixing module, a temperature and humidity sensor group, and a controller. The housing 1 is provided with an openable and closable door 2, and the housing 1 and the door 2 together enclose an internal storage space. The basic cryopreservation box 3 and the multiple extended cryopreservation boxes 4 are disposed within the internal storage space, and the extended cryopreservation boxes 4 are detachably stacked and connected to the bottom or top of the basic cryopreservation box 3 via a snap-fit ​​mechanism. The three-dimensional dynamic mixing module is disposed in the storage cavity within the basic cryopreservation box 3 and the extended cryopreservation boxes 4, and is used to perform mixing operations according to a preset vibration intensity and time. The temperature and humidity sensor group is disposed within the walls of the basic cryopreservation box 3 and the extended cryopreservation boxes 4, and is used to detect temperature and humidity data within the storage space. The controller is disposed within the basic cryopreservation box 3 and is electrically connected to the control terminal of the three-dimensional dynamic mixing module. It is electrically connected to the temperature and humidity sensor group through an electrical connection contact group, and is used to predict temperature change trends and adjust the temperature of the storage space based on temperature and humidity data.

[0053] When storage capacity needs to be increased, the operator simply connects the new extended cryopreservation box 4 to the base cryopreservation box 3 and / or other extended cryopreservation boxes 4 via a snap-fit ​​mechanism, either below or above. When storage capacity needs to be reduced, the operator simply releases the snap-fit ​​mechanism to remove the extended cryopreservation box 4. A three-dimensional dynamic mixing module is used to perform mixing operations on the sample tubes placed in the storage chamber according to preset vibration intensity and time, to prevent component precipitation and stratification of blood samples during long-term storage. Temperature and humidity sensor groups are embedded in the walls of both the base cryopreservation box 3 and the extended cryopreservation box 4. The sensors are distributed in different areas of the walls of each cryopreservation box to achieve multi-point monitoring of temperature and humidity data at different locations within the internal storage space. Electrical contact point groups are correspondingly provided at the bottom and top of the base cryopreservation box 3 and the bottom and top of the extended cryopreservation box 4. When the extended cryopreservation box 4 is stacked and connected to the base cryopreservation box 3 via the snap-fit ​​mechanism, the elastic probe and the contact point contact piece contact each other to achieve power supply and signal transmission. During operation, the controller first acquires real-time temperature and humidity data from the temperature and humidity sensor group via electrical connection contact points and constructs a historical temperature and humidity sequence. Subsequently, the controller inputs this historical sequence into a time series prediction model, which then predicts the temperature and humidity trends over a preset time period. Finally, based on the predicted temperature and humidity trends, the controller adjusts the cooling power of the refrigeration unit or activates the heating unit in advance, ensuring that the temperature of the internal storage space remains within the preset target temperature range. This proactive intervention before temperature anomalies occur effectively prevents the degradation of viral nucleic acids (such as HBV DNA and HCV RNA) or antibody inactivation in blood samples due to sudden temperature changes, significantly improving the stability and reliability of liver disease blood sample storage.

[0054] It should be noted that the snap-fit ​​mechanism, the three-dimensional dynamic mixing module, the temperature and humidity sensor, and the controller are all drawn in the figure.

[0055] Furthermore, in some embodiments, the liver disease blood sample storage system also includes a multi-level threshold comparator, which is set in the basic cryopreservation box and electrically connected to the controller, for comparing temperature and humidity data with preset warning thresholds and triggering an emergency response of the corresponding level.

[0056] It should be noted that the multi-level threshold comparator has multiple preset warning thresholds, including a first-level warning threshold (e.g., temperature deviation from the target value ±2℃), a second-level warning threshold (e.g., temperature deviation from the target value ±5℃), and a third-level warning threshold (e.g., temperature deviation from the target value ±8℃). These warning thresholds can be flexibly set according to the storage requirements of different liver disease blood samples.

[0057] During operation, the multi-level threshold comparator receives real-time temperature and humidity data collected by the temperature and humidity sensor group and / or temperature and humidity prediction values ​​predicted by the time series prediction model in the controller. It then compares these data with multiple preset warning thresholds one by one. Based on the comparison results, the multi-level threshold comparator triggers the corresponding level of emergency response.

[0058] Specifically, when the real-time temperature and humidity data or the predicted temperature and humidity value reaches the first-level warning threshold, the multi-level threshold comparator sends the first-level trigger signal to the controller. After receiving the first-level trigger signal, the controller triggers a local audible and visual alarm to remind on-site operators to pay attention to the abnormal temperature.

[0059] When the real-time temperature and humidity data or the predicted temperature and humidity value reaches the second-level warning threshold, the multi-level threshold comparator sends a second-level trigger signal to the controller. After receiving the second-level trigger signal, the controller sends a notification message to the remote terminal through the wireless communication module, so that the managers can be informed of the abnormal situation as soon as possible.

[0060] When the real-time temperature and humidity data or the predicted temperature and humidity value reaches the third-level warning threshold, the multi-level threshold comparator sends a third-level trigger signal to the controller. After receiving the third-level trigger signal, the controller immediately starts the backup power supply and performs emergency cooling operation to maintain the low temperature environment of the internal storage space in the event of failure of the main cooling device, and to prevent the blood sample from being damaged due to excessive temperature.

[0061] Furthermore, in some embodiments, the liver disease blood sample storage system further includes: an RFID reader / writer, installed on the basic cryopreservation box and the extended cryopreservation box, electrically connected to the controller via an electrical connection contact group, used to scan the RFID tag on the sample tube to obtain the basic information of the sample tube; wherein, the basic information includes: location information, storage duration and sample type.

[0062] Each sample storage tube is equipped with an RFID tag. The RFID tag is a passive UHF RFID tag, which stores a unique identifier for the sample storage tube and associated basic sample information, including location information (e.g., cryopreservation box number, slot number), storage duration (e.g., collection time, storage time), and sample type (e.g., whole blood, serum, plasma).

[0063] An RFID reader / writer includes a radio frequency transceiver module and an antenna. The radio frequency transceiver module generates radio frequency signals and transmits them outward through the antenna. The antenna covers RFID tags within a certain range and receives the signals they return. Depending on the actual needs, the antenna can be installed at the bottom of the cryopreservation box rack, on the side walls of the cryopreservation box rack, or in the middle partition of the cryopreservation box rack.

[0064] During operation, the RFID reader transmits radio frequency signals via its antenna to activate RFID tags within its coverage area. The activated RFID tags then transmit their stored basic information back to the RFID reader via backscatter modulation. Upon receiving the transmitted signal, the RFID reader demodulates and decodes it to obtain the basic information of the sample storage tube, and transmits this information to the controller via electrical contact points. The controller receives this basic information, compares it with records in its database, and updates it, enabling precise location and full lifecycle tracking of each sample storage tube.

[0065] Furthermore, in some embodiments, the three-dimensional dynamic mixing module includes a rocking oscillation structure, a lifting oscillation structure, and a left-right swinging oscillation structure stacked sequentially from bottom to top. A blood vessel placement seat that can slide relative to the left-right swinging oscillation structure is provided on the left-right swinging oscillation structure. The rocking oscillation structure is used to drive the lifting oscillation structure, the left-right swinging oscillation structure, and the blood vessel placement seat to swing back and forth along a first direction. The lifting oscillation structure is used to drive the left-right swinging oscillation structure and the blood vessel placement seat to move up and down along a vertical direction. The left-right swinging oscillation structure is used to drive the blood vessel placement seat to slide back and forth along a second direction. The first direction, the vertical direction, and the second direction constitute three mutually orthogonal directions in a three-dimensional spatial coordinate system.

[0066] The oscillating structure is located at the bottom of the storage cavity and includes an oscillating drive motor and an oscillating transmission mechanism (e.g., an eccentric wheel mechanism or a crank-rocker mechanism). The output shaft of the oscillating drive motor is connected to the input end of the oscillating transmission mechanism, and the output end of the oscillating transmission mechanism is connected to the bottom of the lifting oscillating structure. When the oscillating drive motor is started, its output shaft drives the oscillating transmission mechanism to move. The oscillating transmission mechanism drives the lifting oscillating structure, the left-right oscillating oscillating structure, and the blood vessel placement seat to oscillate back and forth along a first direction (e.g., the X-axis direction). The oscillation amplitude and frequency can be adjusted by controlling the speed and direction of the oscillating drive motor.

[0067] The lifting oscillation structure is mounted on top of the swinging oscillation structure and includes a lifting drive motor and a lifting transmission mechanism (such as a lead screw and nut mechanism or a cam mechanism). The output shaft of the lifting drive motor is connected to the input end of the lifting transmission mechanism, and the output end of the lifting transmission mechanism is connected to the bottom of the left-right swinging oscillation structure. When the lifting drive motor is started, its output shaft drives the lifting transmission mechanism to move, which in turn drives the left-right swinging oscillation structure and the blood vessel placement seat to reciprocate up and down in the vertical direction (such as the Z-axis). The lifting amplitude and frequency can be adjusted by controlling the speed and direction of the lifting drive motor.

[0068] A left-right oscillating oscillation structure is mounted on top of a lifting oscillation structure. It includes a left-right oscillating drive motor and a left-right oscillating transmission mechanism (e.g., a rack and pinion mechanism or a linkage mechanism). The output shaft of the left-right oscillating drive motor is connected to the input end of the left-right oscillating transmission mechanism, and the output end of the left-right oscillating transmission mechanism is connected to the blood vessel placement seat. When the left-right oscillating drive motor is started, its output shaft drives the left-right oscillating transmission mechanism to move. The left-right oscillating transmission mechanism drives the blood vessel placement seat to slide back and forth along a second direction (e.g., the Y-axis direction). The sliding amplitude and frequency can be adjusted by controlling the speed and direction of the left-right oscillating drive motor.

[0069] The vascular placement seat has multiple slots, each for holding a sample collection tube. The vascular placement seat can slide relative to a left-right oscillating structure, allowing the sample collection tubes placed on the vascular placement seat to achieve a reciprocating mixing effect in a second direction.

[0070] Reference Figure 2 , Figure 2 A flowchart illustrating a control method for a liver disease blood sample storage system according to an embodiment of the present invention is shown. In some embodiments, the control method applied to the liver disease blood sample storage system in any of the above embodiments may include, but is not limited to, steps 201 to 203:

[0071] Step 201: Collect temperature and humidity data and construct a historical temperature and humidity sequence.

[0072] Step 202: Input the historical temperature and humidity series into the time series prediction model to predict the temperature and humidity change trend within the preset time period.

[0073] In step 202, the time series prediction model can be a statistical ARIMA model (autoregressive integral moving average model), a machine learning-based LSTM neural network model (long short-term memory network), or a deep learning-based Transformer model, etc.

[0074] Step 203: Adjust the cooling or heating power according to the temperature and humidity change trend.

[0075] Steps 201 to 203 of this embodiment involve collecting temperature and humidity data at the location according to a preset sampling frequency. After receiving the real-time temperature and humidity data, preprocessing is performed, and the preprocessed data is then stored in local storage or a cloud database in chronological order to construct a historical temperature and humidity sequence. This historical sequence is then input into an integrated time-series prediction model. The time-series prediction model analyzes and learns from the input historical temperature and humidity sequence, extracting periodic, trend, and seasonal patterns. Based on these patterns, it predicts the temperature and humidity trends over a preset future time period. When the predicted temperature will exceed the preset target temperature limit within the preset future time period, the cooling power of the refrigeration device is activated or increased in advance to effectively control the temperature of the internal storage space before the predicted temperature rise occurs. When the predicted temperature will fall below the preset target temperature limit within the preset future time period, the cooling power of the refrigeration device is reduced or the heating device is activated in advance to prevent the blood sample from freezing and being damaged due to excessively low temperatures in the internal storage space. When the predicted temperature will remain within the target temperature range for a preset period of time, the current cooling or heating power is maintained to achieve energy-saving operation. This effectively avoids the adverse effects of temperature fluctuations on viral nucleic acid and antibody activity in liver disease blood samples, and improves the quality and reliability of blood sample storage.

[0076] Reference Figure 3 , Figure 3 A flowchart illustrating the control method of a liver disease blood sample storage system according to an embodiment of the present invention is shown. In some embodiments, adjusting the cooling power or heating power according to the temperature and humidity change trend specifically includes, but is not limited to, steps 301 to 302:

[0077] Step 301: When the temperature and humidity change trend indicates that the temperature will exceed the first temperature threshold within a preset time period, start the cooling power in advance or increase it.

[0078] Step 302: When the temperature and humidity change trend indicates that the temperature is below the second temperature threshold within a preset time period, reduce the cooling power or start the heating device. The first temperature threshold is greater than the second temperature threshold.

[0079] In steps 301 to 302 of this embodiment, when the prediction result of the time series prediction model indicates that the temperature of the internal storage space will exceed the first temperature threshold T1 within a preset future time period, it is determined that an overheating situation is about to occur. At this time, before the actual temperature rises, the cooling device is started in advance (if the cooling device is not currently running) or the cooling power of the cooling device is increased (if the cooling device is currently running). By intervening in advance, the temperature of the internal storage space begins to decrease before the predicted temperature rise arrives, thereby controlling the actual temperature within the target temperature range.

[0080] When the time series forecasting model predicts that the temperature of the internal storage space will fall below the second temperature threshold T2 within a preset future time period, it is determined that an excessively low temperature situation is imminent. At this point, before the actual temperature drop, the cooling power of the refrigeration unit is reduced (if the refrigeration unit is currently running) or the heating unit is activated (if the heating unit is not currently running). Through this early intervention, the temperature of the internal storage space begins to rise before the predicted temperature drop occurs, thereby controlling the actual temperature within the target temperature range.

[0081] When the prediction result of the time series forecasting model indicates that the temperature of the internal storage space will remain between the first temperature threshold T1 and the second temperature threshold T2 within a preset time period in the future, the current cooling power or heating power will remain unchanged to achieve energy-saving operation.

[0082] It should be noted that the controller has a preset first temperature threshold T1 and a second temperature threshold T2, where T1 > T2. The first temperature threshold T1 is the upper limit of the target temperature range, and the second temperature threshold T2 is the lower limit of the target temperature range.

[0083] Reference Figure 4 , Figure 4 A flowchart illustrating the control method for a liver disease blood sample storage system according to an embodiment of the present invention is shown. In some embodiments, the optical cable monitoring and change identification method is applied to the optical cable monitoring and change identification system described above, and the control method may further include, but is not limited to, steps 401 to 403:

[0084] Step 401: Determine the three-dimensional dynamic mixing cycle based on the storage time of the sample tube and the sample type.

[0085] Step 402: Predict the quality change trend of the sample tube under different storage conditions using simulation algorithms.

[0086] In step 402, the digital twin is a virtual model built within the controller or on a cloud server. It uses simulation algorithms (such as heat transfer simulation based on finite element analysis, or blood sample component sedimentation simulation based on molecular dynamics) to predict the quality change trend of blood samples in the storage tube under different storage conditions. The quality change trend can be represented as a quality degradation curve, which reflects the changes in key indicators in the blood sample (such as viral nucleic acid concentration, antibody titer, etc.) over storage time.

[0087] Step 403: Dynamically optimize the three-dimensional dynamic mixing cycle according to the quality change trend, and perform the mixing operation when the three-dimensional dynamic mixing cycle is reached.

[0088] In steps 401 to 403 of this embodiment, the basic information stored in the RFID tag on the sample storage tube is read by an RFID reader to obtain the storage duration and sample type of the sample storage tube. Based on the storage duration and sample type, the three-dimensional dynamic mixing cycle of the sample storage tube is determined according to a preset rule.

[0089] Real-time environmental data (including temperature, humidity, mixing history, etc.) and operation records (including entry and exit records, freeze-thaw cycles, etc.) of each sample storage tube are synchronized to the digital twin via a wireless communication module. Based on the real-time environmental data and historical operation records of each sample storage tube, the digital twin simulates the quality degradation curve of each sample storage tube under different mixing cycles using simulation algorithms.

[0090] Specifically, the digital twin simulated the changes in blood sample quality over storage time with different mixing periods of 6 hours, 12 hours, 24 hours, and 48 hours. Then, the digital twin selected the mixing period that minimized the slope of the quality degradation curve from the simulation results as the optimized mixing period—a smaller slope indicates a slower decline in blood sample quality, meaning the mixing period provides better protection for the blood sample quality. The digital twin fed back the optimized mixing period to the controller, which then updated the mixing parameters accordingly.

[0091] Reference Figure 5 , Figure 5 A flowchart illustrating the control method of a liver disease blood sample storage system according to an embodiment of the present invention is shown. In some embodiments, determining the three-dimensional dynamic mixing cycle based on the storage duration and sample type of the sample tube specifically includes, but is not limited to, steps 501 to 503:

[0092] Step 501: For blood samples with a storage time shorter than the first preset time, the three-dimensional dynamic mixing cycle is set as the first cycle.

[0093] Step 502: For blood samples with a storage duration greater than or equal to a first preset duration, the three-dimensional dynamic mixing cycle is shortened to a second cycle. The second cycle is shorter than the first cycle.

[0094] Step 503: When the coagulation risk of the sample type is higher than the preset risk level, the three-dimensional dynamic mixing cycle is set to the third cycle. The third cycle is shorter than the second cycle.

[0095] In step 503, the controller has a pre-set coagulation risk level database, which stores the coagulation risk level corresponding to different sample types. For example, whole blood samples with high platelet counts are marked as high-risk, EDTA-anticoagulated whole blood is marked as medium-risk, and serum samples are marked as low-risk.

[0096] In steps 501 to 503 of this embodiment, when the storage time t of the sample tube is less than the first preset time T0, it indicates that the sample tube has been stored for a short time and the components in the blood sample have not yet undergone significant precipitation and stratification. At this time, the three-dimensional dynamic mixing cycle is set to the first cycle P1 (e.g., 24 hours). That is, a mixing operation is performed every 24 hours, which can prevent blood sample components from precipitating and avoid unnecessary mechanical disturbance to the blood sample caused by overly frequent mixing operations.

[0097] When the storage time t of the sample tube is greater than or equal to the first preset time T0, it indicates that the sample tube has been stored for a relatively long time, and the components in the blood sample have undergone a certain degree of precipitation and stratification. More frequent mixing is required to maintain the homogeneity of the blood sample. At this time, the three-dimensional dynamic mixing cycle is shortened from the first cycle P1 to the second cycle P2 (e.g., 12 hours). That is, a mixing operation is performed every 12 hours to mix the blood sample more frequently and prevent irreversible precipitation of blood sample components caused by long-term storage.

[0098] Once the sample type of the storage tube is obtained, the coagulation risk level database is queried to determine whether the coagulation risk of this sample type is higher than the preset risk level (e.g., higher than the "medium risk" level). If so, it indicates that the blood sample in the storage tube has a high coagulation risk and requires more frequent mixing to prevent coagulation. In this case, the three-dimensional dynamic mixing cycle is set to the third cycle P3 (e.g., 6 hours), where the third cycle P3 is shorter than the first cycle P1 and the second cycle P2. That is, a mixing operation is performed every 6 hours to mix the high-coagulation-risk blood sample at the highest frequency, maximizing the prevention of coagulation.

[0099] Another embodiment of the present invention discloses a control device for a liver disease blood sample storage system, comprising: at least one processor, and a memory communicatively connected to the at least one processor. The memory stores instructions executable by the at least one processor, which, when executed by the at least one processor, enables the at least one processor to perform actions such as... Figure 2 Control method steps S201 to S203 Figure 3 Control method steps S301 and S302 Figure 4 The control method steps S401 to S403 and Figure 5 The control method for the liver disease blood sample storage system in steps S501 to S503.

[0100] Another embodiment of the present invention discloses a computer-readable storage medium, the storage medium comprising: storing computer-executable instructions for causing a computer to perform... Figure 2 Control method steps S201 to S203 Figure 3Control method steps S301 and S302 Figure 4 The control method steps S401 to S403 and Figure 5 The control method for the liver disease blood sample storage system in steps S501 to S503.

[0101] The device embodiments described above are merely illustrative. The units described as separate components may or may not be physically separate; that is, they may be located in one place or distributed across multiple network units. Some or all of the modules can be selected to achieve the purpose of this embodiment according to actual needs.

[0102] It will be understood by those skilled in the art that all or some of the steps and systems in the methods disclosed above can be implemented as software, firmware, hardware, and suitable combinations thereof. Some or all of the physical components can be implemented as software executed by a processor, such as a central processing unit, digital signal processor, or microprocessor, or as hardware, or as an integrated circuit, such as an application-specific integrated circuit. Such software can be distributed on a computer-readable medium, which can include computer storage media (or non-transitory media) and communication media (or transient media). As is known to those skilled in the art, the term computer storage media includes volatile and non-volatile, removable and non-removable media implemented in any method or technology for storing information (such as computer-readable instructions, data structures, program modules, or other data). Computer storage media includes, but is not limited to, RAM, ROM, EEPROM, flash memory or other memory technologies, CD-ROM, digital versatile disc (DVD) or other optical disc storage, magnetic cartridges, magnetic tape, disk storage or other magnetic storage devices, or any other medium that can be used to store desired information and is accessible to a computer. Furthermore, as is known to those skilled in the art, communication media typically contain computer-readable instructions, data structures, program modules, or other data in modulated data signals such as carrier waves or other transmission mechanisms, and may include any information delivery medium.

[0103] The embodiments of the present invention have been described in detail above with reference to the accompanying drawings. However, the present invention is not limited to the above embodiments, and various changes can be made within the scope of knowledge possessed by those skilled in the art without departing from the spirit of the present invention. Furthermore, the embodiments of the present invention and the features thereof can be combined with each other unless otherwise specified.

Claims

1. A blood sample storage system for liver disease, characterized in that, include: The box body is equipped with an openable and closable door, and the box body and the door together enclose an internal storage space; A basic cryopreservation box and multiple extended cryopreservation boxes are disposed within the internal storage space. The extended cryopreservation boxes are detachably stacked and connected to the basic cryopreservation box below or above it via a snap-fit ​​mechanism. A three-dimensional dynamic mixing module is installed in the storage cavity within the basic cryopreservation box and the extended cryopreservation box, and is used to perform mixing operations according to preset vibration intensity and time. A temperature and humidity sensor array is installed in the walls of the basic cryopreservation box and the extended cryopreservation box to detect temperature and humidity data within the storage space. The controller is located inside the basic cryogenic storage box and is electrically connected to the control terminal of the three-dimensional dynamic mixing module. It is also electrically connected to the temperature and humidity sensor group through an electrical contact point group. The controller is used to predict the temperature change trend based on the temperature and humidity data and adjust the temperature of the storage space.

2. The liver disease blood sample storage system according to claim 1, characterized in that, The liver disease blood sample storage system also includes a multi-level threshold comparator, which is installed in the basic cryopreservation box and electrically connected to the controller. It is used to compare the temperature and humidity data with preset warning thresholds and trigger the corresponding level of emergency response.

3. The liver disease blood sample storage system according to claim 1, characterized in that, The liver disease blood sample storage system further includes an RFID reader / writer, which is installed on the basic cryopreservation box and the extended cryopreservation box, and is electrically connected to the controller through the electrical contact point group. The reader / writer is used to scan the RFID tag on the sample storage tube to obtain the basic information of the sample storage tube. The basic information includes: location information, storage duration, and sample type.

4. The liver disease blood sample storage system according to claim 1, characterized in that, The three-dimensional dynamic mixing module includes a rocking oscillation structure, a lifting oscillation structure, and a left-right swinging oscillation structure stacked sequentially from bottom to top. A blood vessel placement seat that can slide relative to the left-right swinging oscillation structure is provided on the left-right swinging oscillation structure. The rocking oscillation structure is used to drive the lifting oscillation structure, the left-right swinging oscillation structure, and the blood vessel placement seat to swing back and forth along a first direction. The lifting oscillation structure is used to drive the left-right swinging oscillation structure and the blood vessel placement seat to move up and down along a vertical direction. The left-right swinging oscillation structure is used to drive the blood vessel placement seat to slide back and forth along a second direction. The first direction, the vertical direction, and the second direction constitute three mutually orthogonal directions in a three-dimensional spatial coordinate system.

5. A control method for a liver disease blood sample storage system, characterized in that, The control method, applied to the liver disease blood sample storage system according to any one of claims 1 to 4, comprises: Collect temperature and humidity data and construct historical temperature and humidity sequences; The historical temperature and humidity series is input into the time series prediction model to predict the temperature and humidity change trend within a preset time period. Adjust the cooling or heating power according to the temperature and humidity change trend.

6. The control method according to claim 5, characterized in that, The adjustment of cooling or heating power based on the temperature and humidity change trend includes: When the temperature and humidity change trend indicates that it will exceed the first temperature threshold within a preset time period, the cooling power will be started in advance or increased. When the temperature and humidity change trend indicates that the temperature is below the second temperature threshold within the preset time period, the cooling power is reduced or the heating device is activated; wherein, the first temperature threshold is greater than the second temperature threshold.

7. The control method according to claim 5, characterized in that, Also includes: The three-dimensional dynamic mixing cycle is determined based on the storage time of the sample tube and the sample type. The quality change trend of the sample tube under different storage conditions is predicted by simulation algorithm; The three-dimensional dynamic mixing cycle is dynamically optimized based on the quality change trend, and a mixing operation is performed when the three-dimensional dynamic mixing cycle is reached.

8. The control method according to claim 7, characterized in that, The determination of the three-dimensional dynamic mixing cycle based on the storage time and sample type of the sample storage tube includes: For blood samples whose storage time is less than a first preset time, the three-dimensional dynamic mixing cycle is set as the first cycle. For blood samples with a storage duration greater than or equal to the first preset duration, the three-dimensional dynamic mixing cycle is shortened to a second cycle; wherein the second cycle is shorter than the first cycle; When the coagulation risk of the sample type is higher than the preset risk level, the three-dimensional dynamic mixing cycle is set to the third cycle; wherein the third cycle is shorter than the second cycle.

9. A control device for a liver disease blood sample storage system, characterized in that, include: At least one processor, and, A memory communicatively connected to the at least one processor; wherein, The memory stores instructions executable by the at least one processor, which, when executed by the at least one processor, enables the at least one processor to perform the control method for the liver disease blood sample storage system as described in any one of claims 5 to 8.

10. A computer-readable storage medium, characterized in that, The computer-readable storage medium stores computer-executable instructions for causing a computer to perform a control method for the liver disease blood sample storage system as described in any one of claims 5 to 8.

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