Multifunctional synergistic fertilizer based on inorganic-organic-microorganism cooperation and preparation method thereof

Abstract

The application discloses a kind of multifunctional synergistic fertilizer based on inorganic-organic-microorganism cooperation and preparation method thereof, and it relates to the technical field of fertilizer, the fertilizer includes yeast source biochemical potassium fulvate powder, mineral source potassium fulvate powder, potassium dihydrogen phosphate, ammonium sulfate, ethylenediaminetetraacetic acid chelated calcium, ethylenediaminetetraacetic acid chelated magnesium, ethylenediaminetetraacetic acid chelated zinc, ethylenediaminetetraacetic acid chelated iron, disodium octaborate tetrahydrate, compound microbial inoculant, gamma-aminobutyric acid, D-allose, green algae enzymatic extract, succinic acid bisdiethylaminoethanol citrate and polyglutamic acid by weight parts;The compound microbial inoculant includes arbuscular mycorrhizal fungi inoculant, bacillus mucilaginosus inoculant, paenibacillus polymyxa inoculant and bacillus megaterium inoculant.The fertilizer of the application can realize nutrient supply, rhizosphere environment regulation and soil physical and chemical property improvement, and can be used to promote crop root growth, improve stress resistance and improve crop yield and quality.

Description

Technical Field

[0001] This invention relates to the field of fertilizer technology, specifically to a multifunctional synergistic fertilizer based on inorganic-organic-microbial synergy and its preparation method. Background Technology

[0002] With the development of facility agriculture, intensive cultivation, and integrated water and fertilizer technology, the requirements for fertilizers in agricultural production have gradually shifted from simply supplementing nitrogen, phosphorus, and potassium to a focus on comprehensive nutrient supply, rhizosphere environment regulation, and ease of application. During long-term continuous cultivation of greenhouse vegetables, concentrated fertilizer input and frequent irrigation can easily lead to soil degradation, salt accumulation, decreased soil structural stability, and changes in rhizosphere microbial composition. Under partial continuous cropping conditions, the number of beneficial microorganisms in the soil decreases, while soil-borne pathogens accumulate, potentially further affecting crop root growth, nutrient absorption, and yield formation. Therefore, while meeting the macronutrient requirements of crops, the synergistic supply of micronutrients, organic functional components, and beneficial microorganisms has become an important direction for the development of fertilizers in facility agriculture.

[0003] In current agricultural production, macronutrient fertilizers, micronutrient fertilizers, organic functional fertilizers, and microbial inoculants are typically produced and applied separately. Among these, water-soluble macronutrient fertilizers primarily provide nitrogen, phosphorus, and potassium, featuring high nutrient concentrations and convenient application. However, they usually contain little or no organic functional substances and active microorganisms, limiting their regulatory effect on rhizosphere microecology and soil physical properties. Micronutrient fertilizers can supplement the nutrients required by crops, such as calcium, magnesium, iron, zinc, and boron. However, some common inorganic micronutrients are prone to precipitation, fixation, or ion interactions in complex systems containing high concentrations of phosphate and sulfate ions, thus affecting the dispersion stability of the fertilizer system and the effective utilization of the corresponding elements. Organic functional fertilizers such as humic acid, fulvic acid, amino acids, and algal extracts can introduce organic matter or bioactive substances into the fertilizer system, but they usually cannot independently meet the crop's demand for large amounts of nitrogen, phosphorus, and potassium during its rapid growth phase. Microbial inoculants can utilize the effects of beneficial microorganisms during rhizosphere colonization and metabolism, participating in soil nutrient transformation and rhizosphere environment regulation. However, the activity of microbial agents is easily affected by the external environment.

[0004] In recent years, some compound fertilizers have attempted to combine inorganic nutrients, organic functional substances, and microbial agents into the same product. However, most compound fertilizers are simply physically mixed and suffer from problems such as unreasonable microbial compatibility, antagonism between organic and inorganic substances, and inactivation of microorganisms due to high-temperature sterilization. As a result, they cannot achieve the integrated effects of improving soil, promoting root growth, disease resistance, stress resistance, quality improvement, and yield increase. Summary of the Invention

[0005] The purpose of this invention is to provide a multifunctional synergistic fertilizer based on inorganic-organic-microbial synergy. By combining macro-elements, micro-elements, a dual-source fulvic acid system, bioactive components, and specific compound microbial agents, it improves the fertilizer's comprehensive nutrient supply capacity and application adaptability, and addresses the problem of relatively limited functions of simple inorganic fertilizers, organic functional fertilizers, or single microbial agents.

[0006] Another objective of this invention is to provide a method for preparing the aforementioned multifunctional synergistic fertilizer. Through a phased processing approach involving the premixing of inorganic nutrient components, the premixing of organic functional components, the compounding of the two, and the subsequent addition of compound microbial agents, the microorganisms are prevented from participating in the initial pulverization process and their exposure time to localized high salt, high mechanical action, and processing temperature rise environments is reduced. This helps to maintain the biological activity of functional microorganisms in the finished product and the storage stability of the product.

[0007] To solve the above-mentioned technical problems, the technical solution of the present invention is as follows:

[0008] Firstly, a multifunctional synergistic fertilizer based on inorganic-organic-microbial synergy, comprising the following components by weight:

[0009] The following components are included: 6-15 parts of yeast-derived biochemical potassium humate powder, 8-14 parts of mineral-derived potassium humate powder, 25-35 parts of potassium dihydrogen phosphate, 30-40 parts of ammonium sulfate, 0.2-0.7 parts of EDTA-chelated calcium, 1-3 parts of EDTA-chelated magnesium, 0.2-0.7 parts of EDTA-chelated zinc, 0.6-1.5 parts of EDTA-chelated iron, 0.1-0.5 parts of disodium octaborate tetrahydrate, 2-6 parts of compound microbial inoculant, 0.1-0.5 parts of γ-aminobutyric acid, 0.1-0.4 parts of D-allose, 3-7 parts of enzymatic hydrolysis extract of green algae, 0.1-0.4 parts of diethylaminoethanol succinate citrate, and 2-6 parts of polyglutamic acid.

[0010] The compound microbial inoculant includes arbuscular mycorrhizal fungi inoculant, Bacillus mucilaginosus inoculant, Bacillus polymyxa inoculant, and Bacillus megaterium inoculant.

[0011] Preferably, the total effective viable count of *Bacillus mucilaginosus*, *Bacillus polymyxa*, and *Bacillus megaterium* in the compound microbial agent is not less than 7.5 × 10⁻⁶. 8 CFU / g, wherein the effective propagule content in the arbuscular mycorrhizal fungal inoculum is ≥50 cells / g of inoculum.

[0012] In this invention, the effective propagules of arbuscular mycorrhizal fungi are determined using the maximum probable number method (MPN infection potential method). Clover is selected as the indicator plant, sterilized river sand is used as the substrate, and the cultivation period is 45 days. The number of effective propagules is calculated based on the root infection status.

[0013] Preferably, the mass ratio of the arbuscular mycorrhizal fungal agent, Bacillus mucilaginosus agent, Bacillus polymyxa agent, and Bacillus megaterium agent is (5-8):(1-2):(1-2):(0.5-1.5).

[0014] Preferably, the green algae enzymatic hydrolysis extract is a water-soluble or water-dispersible extract obtained by breaking down and enzymatically hydrolyzing green algae raw materials.

[0015] Preferably, the yeast-derived biochemical potassium humate powder is potassium humate powder obtained by separating, concentrating and drying yeast fermentation products; the mineral-derived potassium humate powder is potassium humate powder extracted from lignite, weathered coal or peat as raw materials.

[0016] Secondly, a method for preparing a multifunctional synergistic fertilizer based on inorganic-organic-microbial synergy includes the following steps:

[0017] S1. Mix potassium dihydrogen phosphate, ammonium sulfate, calcium chelated with ethylenediaminetetraacetic acid (EDTA), magnesium chelated with EDTA, zinc chelated with EDTA, iron chelated with EDTA, and disodium octaborate tetrahydrate, pulverize and pass through an 80-mesh sieve to obtain an inorganic nutrient mixture.

[0018] S2. Mix yeast-derived biochemical potassium humate powder, green algae enzymatic hydrolysis extract, γ-aminobutyric acid, D-aloose, mineral-derived potassium humate powder, diethylaminoethanol succinate citrate, and polyglutamic acid to obtain an organic functional mixture.

[0019] S3. Stir and mix the inorganic nutrient mixture with the organic functional mixture to obtain a premix;

[0020] S4. Add compound microbial inoculant to the premix, stir and mix, and finally package to obtain a multifunctional and efficient fertilizer.

[0021] Preferably, in step S2, the mixing time is 20-30 min and the stirring speed is 40-60 r / min.

[0022] Preferably, in step S3, the mixing time is 15-25 min and the stirring speed is 12-20 r / min.

[0023] Preferably, in step S4, the mixing time is 8-15 min and the stirring speed is 8-15 r / min.

[0024] Preferably, the material temperature in steps S2 to S4 is 15 to 30°C, and the highest material temperature during the preparation process does not exceed 35°C.

[0025] By adopting the above technical solution, the present invention has at least the following beneficial effects:

[0026] This invention combines potassium dihydrogen phosphate, ammonium sulfate, chelated calcium, magnesium, zinc, iron, and boron sources to provide fertilizers that supply nitrogen, phosphorus, potassium, and various micronutrients. The calcium, magnesium, zinc, and iron are in the form of ethylenediaminetetraacetic acid chelates, which reduces the occurrence of insoluble substances forming directly in phosphorus-containing systems and improves the dispersion stability of micronutrients in fertilizer solutions.

[0027] The fertilizer of this invention contains enzymatically hydrolyzed green algae extract, γ-aminobutyric acid (GABA), D-allose, diethylaminoethanol succinate citrate, and polyglutamic acid organic components. These components provide crops and functional microorganisms with amino acids, small-molecule organic matter, and bioactive ingredients, which are beneficial for promoting root development, maintaining leaf photosynthetic capacity, and improving crop adaptability to adverse environments such as low temperature, low light, and salt stress. Polyglutamic acid also enhances the water and fertilizer retention capacity of the fertilizer system, reduces nutrient leaching, and promotes the formation and stability of soil aggregates.

[0028] This invention utilizes arbuscular mycorrhizal fungi, Bacillus mucilaginosus, Bacillus polymyxa, and Bacillus megaterium to construct a composite microbial inoculant. Different functional microorganisms can respectively promote root absorption, activate poorly utilized phosphorus and potassium nutrients in the soil, improve the rhizosphere microecology, and inhibit soil-borne harmful microorganisms. By adjusting the ratio between different inoculants, this invention helps reduce nutrient competition and metabolic antagonism among microbial species, expands the functional range of the microbial inoculant, and improves the adaptability and functional stability of the composite microbial community in the soil environment.

[0029] The fertilizer of this invention employs a staged mixing method involving inorganic nutrients, organic functional components, and a compound microbial agent. First, an inorganic nutrient mixture and an organic functional mixture are prepared separately, then compounded, with the compound microbial agent added in the final stage of preparation. Compared to a one-time high-speed mixing of all raw materials, this invention reduces the time that functional microorganisms are in direct contact with locally high-salt materials, avoiding damage to microbial activity caused by crushing, high-speed stirring, and material heating. This is beneficial for increasing the number of effective viable bacteria in the finished product and the viability retention rate during storage. Furthermore, this invention adds the compound microbial agent at a lower temperature and lower stirring speed, and controls the maximum material temperature during preparation to not exceed 35°C. This reduces the damage to Bacillus and arbuscular mycorrhizal fungi propagules caused by mechanical shearing and temperature rise, improving the fertilizer's dispersion uniformity and storage stability. Detailed Implementation

[0030] To better understand the above-mentioned objectives, features, and advantages of the present invention, the solutions of the present invention will be further described below. It should be noted that, unless otherwise specified, the embodiments of the present invention and the features thereof can be combined with each other.

[0031] Many specific details are set forth in the following description in order to provide a full understanding of the invention, but the invention may also be practiced in other ways different from those described herein; obviously, the embodiments in the specification are only some embodiments of the invention, and not all embodiments.

[0032] The raw materials used in the following examples and comparative examples are all commercially available products. Unless otherwise specified, each raw material meets the corresponding product quality standards.

[0033] The yeast-derived biochemical potassium fulvate powder was provided by Shandong Puyuan Bioengineering Co., Ltd., with fulvate content ≥50%, potassium content ≥10%, and water-insoluble matter ≤5%.

[0034] The mineral-derived potassium fulvic acid powder was provided by Hongtong County Wanhuai Humic Acid Processing Co., Ltd., with a fulvic acid content ≥55%, a potassium content ≥8%, and water-insoluble matter ≤3%.

[0035] The enzymatic hydrolysis extract of green algae was provided by Qingdao Chengyi Marine Biotechnology Co., Ltd., with a free amino acid content of ≥15% and an alginic acid content of ≥5%.

[0036] Polyglutamic acid: γ-polyglutamic acid, purchased from Shandong Jingwei Chemical Co., Ltd., with a molecular weight of 100,000-300,000;

[0037] Calcium chelated with ethylenediaminetetraacetic acid (EDTA): calcium content 9.65%; magnesium chelated with EDTA: purchased from Shandong Siyang Biotechnology Co., Ltd.: Mg content 5.98%; zinc chelated with EDTA: Zn content 14.95%; iron chelated with EDTA: Fe content 13.02%.

[0038] Diethylaminoethanol succinate citrate: 98% purity, purchased from Kaifeng Jiangshuo Agricultural Technology Co., Ltd.;

[0039] The sources of each microbial agent in the compound microbial agent are as follows:

[0040] Bacillus mucilaginosus inoculum: purchased from Shandong Jingwei Chemical Co., Ltd. (item number 06), effective viable count ≥ 5 billion CFU / g;

[0041] Bacillus polymyxa inoculum: purchased from Shandong Zhongyi Chemical Technology Co., Ltd. (item number 111), effective viable count ≥10 billion CFU / g;

[0042] Bacillus megaterium inoculum, purchased from Shandong Jingwei Chemical Co., Ltd. (item number 2354), with an effective viable count ≥10 billion CFU / g;

[0043] Arbuscular mycorrhizal fungi: Shandong Chenhe Biotechnology Co., Ltd., Product Code: CH-AM-001.

[0044] Unless otherwise stated, all other raw materials are commercially available.

[0045] The formulations of the following embodiments are shown in Table 1, by weight:

[0046] Table 1

[0047]

[0048] In Example 1, the mass ratio of arbuscular mycorrhizal fungi inoculant, Bacillus mucilaginosus inoculant, Bacillus polymyxa inoculant, and Bacillus megaterium inoculant was 6:1.5:1.5:1;

[0049] In Example 2, the mass ratio of arbuscular mycorrhizal fungi inoculant, Bacillus mucilaginosus inoculant, Bacillus polymyxa inoculant, and Bacillus megaterium inoculant was 5:1:1:0.5.

[0050] In Example 3, the mass ratio of arbuscular mycorrhizal fungi inoculant, Bacillus mucilaginosus inoculant, Bacillus polymyxa inoculant, and Bacillus megaterium inoculant was 8:2:2:1.5;

[0051] In Example 4, the mass ratio of arbuscular mycorrhizal fungi inoculant, Bacillus mucilaginosus inoculant, Bacillus polymyxa inoculant, and Bacillus megaterium inoculant was 7:2:1.5:1;

[0052] In Example 5, the mass ratio of arbuscular mycorrhizal fungi inoculant, Bacillus mucilaginosus inoculant, Bacillus polymyxa inoculant, and Bacillus megaterium inoculant was 6:1.5:1.5:1.

[0053] Example 1

[0054] A method for preparing a multifunctional synergistic fertilizer based on inorganic-organic-microbial synergy includes the following steps:

[0055] S1. Weigh out potassium dihydrogen phosphate, ammonium sulfate, calcium chelated with ethylenediaminetetraacetic acid (EDTA), magnesium chelated with EDTA, zinc chelated with EDTA, iron chelated with EDTA, and disodium octaborate tetrahydrate according to the weight parts of Example 1 in Table 1. Add each component to a mixing device for preliminary mixing, then pulverize and pass through an 80-mesh sieve to obtain an inorganic nutrient mixture.

[0056] S2. Weigh out yeast-derived biochemical potassium fulvate powder, mineral-derived potassium fulvate powder, green algae enzymatic hydrolysis extract, γ-aminobutyric acid, D-aloose, diethylaminoethanol succinate citrate and polyglutamic acid, add them to the mixing equipment at a material temperature of 20℃, and stir at 50r / min for 25min to obtain an organic functional mixture.

[0057] S3. Mix the inorganic nutrient mixture obtained in step S1 with the organic functional mixture obtained in step S2, and stir at 16 r / min at 20°C for 20 min to fully disperse the inorganic nutrient components and organic functional components to obtain a premix.

[0058] S4. Control the temperature of the premix to not exceed 30℃, add compound microbial agent to the premix, and stir at 10r / min for 10min; after mixing, seal and package to obtain multifunctional and efficient fertilizer.

[0059] Example 2

[0060] A method for preparing a multifunctional synergistic fertilizer based on inorganic-organic-microbial synergy includes the following steps:

[0061] S1. Weigh each component according to the weight parts of Example 2 in Table 1, and other conditions are the same as S1 in Example 1;

[0062] S2. Weigh out yeast-derived biochemical potassium fulvate powder, mineral-derived potassium fulvate powder, green algae enzymatic hydrolysis extract, γ-aminobutyric acid, D-allose, diethylaminoethanol succinate citrate and polyglutamic acid, add them to the mixing equipment at a material temperature of 20℃, and stir at 40r / min for 30min to obtain an organic functional mixture.

[0063] S3. Mix the inorganic nutrient mixture obtained in step S1 with the organic functional mixture obtained in step S2, and stir at 12 r / min at 25°C for 25 min to fully disperse the inorganic nutrient components and organic functional components to obtain a premix.

[0064] S4. Control the temperature of the premix to not exceed 30℃, add compound microbial agent to the premix, and stir at 8r / min for 15min; after mixing, seal and package to obtain multifunctional and efficient fertilizer.

[0065] Example 3

[0066] A method for preparing a multifunctional synergistic fertilizer based on inorganic-organic-microbial synergy includes the following steps:

[0067] S1. Weigh each component according to the weight parts of Example 3 in Table 1, and other conditions are the same as S1 in Example 1;

[0068] S2. Weigh out yeast-derived biochemical potassium fulvate powder, mineral-derived potassium fulvate powder, green algae enzymatic hydrolysis extract, γ-aminobutyric acid, D-allose, diethylaminoethanol succinate citrate and polyglutamic acid, add them to the mixing equipment at a material temperature of 25℃, and stir at 60r / min for 20min to obtain an organic functional mixture.

[0069] S3. Mix the inorganic nutrient mixture obtained in step S1 with the organic functional mixture obtained in step S2, and stir at 20 r / min at 25°C for 15 min to fully disperse the inorganic nutrient components and organic functional components to obtain a premix.

[0070] S4. Control the temperature of the premix to not exceed 30℃, add compound microbial agent to the premix, and stir at 15r / min for 8min; after mixing, seal and package to obtain multifunctional and efficient fertilizer.

[0071] Example 4

[0072] A method for preparing a multifunctional synergistic fertilizer based on inorganic-organic-microbial synergy includes the following steps:

[0073] S1. Weigh each component according to the weight parts of Example 4 in Table 1, and other conditions are the same as S1 in Example 1.

[0074] S2. Weigh out yeast-derived biochemical potassium fulvate powder, mineral-derived potassium fulvate powder, green algae enzymatic hydrolysis extract, γ-aminobutyric acid, D-aloose, diethylaminoethanol succinate citrate and polyglutamic acid, add them to the mixing equipment at a material temperature of 20℃, and stir at a speed of 45r / min for 28min to obtain an organic functional mixture.

[0075] S3. Mix the inorganic nutrient mixture obtained in step S1 with the organic functional mixture obtained in step S2, and stir at 18 r / min at 25°C for 18 min to fully disperse the inorganic nutrient components and organic functional components to obtain a premix.

[0076] S4. Control the temperature of the premix to not exceed 30°C, add compound microbial agent to the premix, and stir at 12r / min for 12min; after mixing, seal and package to obtain multifunctional and efficient fertilizer.

[0077] Example 5

[0078] A method for preparing a multifunctional synergistic fertilizer based on inorganic-organic-microbial synergy includes the following steps:

[0079] S1. Weigh each component according to the weight parts of Example 5 in Table 1, and other conditions are the same as S1 in Example 1;

[0080] S2. Weigh out yeast-derived biochemical potassium fulvate powder, mineral-derived potassium fulvate powder, green algae enzymatic hydrolysis extract, γ-aminobutyric acid, D-aloose, diethylaminoethanol succinate citrate and polyglutamic acid, add them to the mixing equipment at a material temperature of 20℃, and stir at 50r / min for 25min to obtain an organic functional mixture.

[0081] S3. Mix the inorganic nutrient mixture obtained in step S1 with the organic functional mixture obtained in step S2, and stir at 16 r / min at 20°C for 20 min to fully disperse the inorganic nutrient components and organic functional components to obtain a premix.

[0082] S4. Control the temperature of the premix to not exceed 30℃, add compound microbial agent to the premix, and stir at 10r / min for 10min; after mixing, seal and package to obtain multifunctional and efficient fertilizer.

[0083] Comparative Example 1

[0084] Conventional inorganic fertilizer with the same N, P2O5, and K2O input levels as in Example 1 was used, and a commercially available compound microbial agent with the same mass as the microbial agent added in Example 1 was added. The commercially available compound microbial agent was a mixture of Bacillus subtilis and Bacillus licheniformis in a 1:1 mass ratio, and its total effective viable bacteria count was adjusted to be the same as that in the compound microbial agent of Example 1. All materials were added to a mixing device at once, stirred at 50 rpm for 25 minutes at 20°C, and then sealed and packaged.

[0085] Comparative Example 2

[0086] The difference between this example and Example 1 is that: the homologous inactivation carrier of Bacillus megaterium inoculant is used to replace Bacillus megaterium inoculant in an equal amount; the mass ratio of arbuscular mycorrhizal fungi inoculant, Bacillus mucilaginosus inoculant, and Bacillus polymyxa inoculant is 6:1.5:1.5; and the amount of compound microbial inoculant and other conditions are the same as in Example 1.

[0087] Comparative Example 3

[0088] The difference between this example and Example 1 is that: the homologous inactivation carrier of Bacillus polymyxa inoculant is used to replace Bacillus polymyxa inoculant in an equal amount; the mass ratio of arbuscular mycorrhizal fungi inoculant, Bacillus mucilaginosus inoculant, and Bacillus megaterium inoculant is 6:1.5:1; and the amount of compound microbial inoculant and other conditions are the same as in Example 1.

[0089] Comparative Example 4

[0090] The difference between this example and Example 1 is that: the homologous inactivation carrier of Bacillus mucilaginosus agent is used to replace the Bacillus mucilaginosus agent in an equal amount; the mass ratio of arbuscular mycorrhizal fungi agent, Bacillus polymyxa agent, and Bacillus megaterium agent is 6:1.5:1; and the amount of compound microbial agent and other conditions are the same as in Example 1.

[0091] Comparative Example 5

[0092] The difference between this example and Example 1 is that: an equal amount of homologous inactivated carrier of arbuscular mycorrhizal fungi agent is used to replace the arbuscular mycorrhizal fungi agent; the mass ratio of Bacillus mucilaginosus agent, Bacillus polymyxa agent, and Bacillus megaterium agent is 1.5:1.5:1; and the amount of compound microbial agent and other conditions are the same as in Example 1.

[0093] Comparative Example 6

[0094] The difference between this example and Example 1 is that, based on Example 1, no compound microbial agent is added, while other conditions are the same as in Example 1.

[0095] Comparative Example 7

[0096] The difference between this example and Example 1 is that, during preparation, all components are added to the mixing device at once, and stirred continuously at 50 r / min for 25 min at 20°C. After mixing, the mixture is sealed and packaged. Other conditions are the same as in Example 1.

[0097] Test example:

[0098] All examples and comparative examples were performed in triplicate. The overall field trial design followed NY / T 1536-2025 "Technical Specifications for Field Trials and Effect Evaluation of Microbial Fertilizers" and NY / T 2544-2014 "General Requirements for Fertilizer Effect Testing and Evaluation". To minimize the impact of differences in basal nutrient input between treatments, crop variety, planting density, irrigation system, cultivation management, and environmental conditions were kept consistent across treatments, except for the application of the tested fertilizer. When additional nitrogen, phosphorus, and potassium fertilizers were required, they were supplemented according to the principle of nutrient balance to ensure consistent basal nitrogen, phosphorus, and potassium input levels across all treatments throughout their growth period.

[0099] I. Storage stability test:

[0100] The fertilizers from the above embodiments and comparative examples were sealed in the same packaging material and stored in an environment at 25±2℃ and relative humidity not exceeding 60%. The total effective viable number of Bacillus bacteria was measured at 0 months and 6 months of storage, and the root infection ability of arbuscular mycorrhizal fungi was also tested.

[0101] (1) The effective viable count of Bacillus bacteria was determined according to NY / T 3264-2018 "Determination of Bacillus in Agricultural Microbial Agents" and NY / T 2321-2013 "Inspection Procedures for Microbial Fertilizer Products". A bacterial suspension was prepared from the fertilizer sample to be tested, and after serial dilution, it was plate-cultured on a suitable culture medium. Bacillus mucilaginosus, Bacillus polymyxa, and Bacillus megaterium were cultured under conventional identification culture conditions adapted to their growth characteristics. For colonies whose morphology could be clearly distinguished, a preliminary judgment was made based on colony morphology, color, transparency, and culture characteristics; for colonies that could not be reliably distinguished solely by colony morphology, further physiological and biochemical characteristics or molecular identification were used to confirm the species. The total effective viable count of Bacillus mucilaginosus, Bacillus polymyxa, and Bacillus megaterium was calculated by summing the counts and expressed as CFU / g.

[0102] The 6-month viable cell retention rate is calculated using the following formula:

[0103] Viable bacteria retention rate (%) = Total number of effective viable bacteria after 6 months of storage / Total number of effective viable bacteria after 0 months of storage × 100%.

[0104] (2) The bioactivity of arbuscular mycorrhizal fungi after storage was evaluated by host plant trapping culture combined with root infection detection. The specific method was as follows: 10g of the fertilizer sample to be tested was mixed with 90g of sterilized river sand (average particle size 1.5mm, high-pressure steam sterilization at 121℃ for 1h) and placed in seedling pots; 50 seeds were sown in each pot using clover as the host plant and the seedlings were grown at 25±2℃ and light intensity 200μmol·m -2 ·s -1 The roots were cultured for 45 days under a 16h / 8h photoperiod. Half Hoogland's nutrient solution was added every 3 days during the culture period. After the culture was completed, newly formed fine roots of the host were randomly collected, washed, and incubated in a 90℃ water bath with 10wt% KOH for 60 min to allow them to clear. They were then neutralized with 1wt% HCl solution and stained with 0.05wt% tribenzene blue at 90℃ for 15 min, and observed under a stereomicroscope. At least 100 root segments were randomly observed in each replicate. A positive result was defined as the observation of arbuscular mycelium, vesicles, or typical hyphal structures in the root segments.

[0105] Root infection rate is calculated using the following formula:

[0106] Root infection rate (%) = number of root segments infected by arbuscular mycorrhizal fungi / total number of observed root segments × 100%. The test results are shown in Table 2.

[0107] Table 2

[0108]

[0109] As shown in Table 2, the viability retention rate of the three functional bacteria in Examples 1-5 after 6 months of storage was approximately 79.6%-84.6%, while that of Comparative Example 7, which used the same formulation as Example 1 but was mixed in a single batch, was only 50.0%. The AMF root infection rate of Example 1 decreased from 68.0% at 0 months to 54.2% at 6 months, but was still significantly higher than the 25.4% of Comparative Example 7. These results indicate that adding the compound microbial agent later and using a mixing method with lower rotation speed and lower temperature conditions helps reduce the impact of processing on microbial activity and improves the viability retention level after storage.

[0110] II. Fertilizer Dispersion Stability:

[0111] Weigh 20g of fertilizer sample, add 200mL of deionized water, stir at 200r / min for 30min at 25℃, then let stand for 24h, observe the appearance of the solution, and record whether layering, turbidity, or precipitation occurs; the conductivity of the supernatant is measured using a conductivity meter; the method for determining the amount of precipitate is as follows: wash the precipitate at the bottom of the container three times with deionized water, dry it at 105℃ to constant weight, and weigh it; each treatment is repeated three times, and the average value is taken. The test results are shown in Table 3.

[0112] Table 3

[0113]

[0114] As shown in Table 3, the 24-hour dry matter precipitation in Examples 1-5 was 0.02-0.05 g / 200 mL. The dry matter precipitation in Comparative Example 1 was 1.85 g / 200 mL, significantly higher than in the other examples. Example 1 and Comparative Example 7 had the same formulation, but the dry matter precipitation in Comparative Example 7 was 0.12 g / 200 mL. These results indicate that a staged mixing process for different components helps maintain good water dispersion of the product and reduces agglomeration and sedimentation that occur under single-stage mixing conditions.

[0115] III. Soil Experiment with Continuous Crop Tomatoes:

[0116] A greenhouse where tomatoes had been continuously grown for three years was selected as the experimental site, and each treatment was set up with three replicates using a randomized block design. The control group used the fertilizers from each comparative example, while the experimental groups used the fertilizers from each example. Except for the fertilizer treatments, the tomato varieties, planting densities, irrigation systems, and other cultivation management conditions used in each treatment were kept consistent. To reduce the impact of differences in basal nutrient input between different treatments on the experimental results, the nitrogen, P2O5, and K2O inputs for each treatment group were calculated based on the actual nutrient composition of the fertilizers tested, and supplemented with basal fertilizers from the same source to ensure that the basal nitrogen, phosphorus, and potassium supply levels were consistent across all treatment groups within the same field experiment.

[0117] The specific procedures for fertilizer application are as follows:

[0118] (1) Application during the transplanting and survival period: After the tomato seedlings have been transplanted and survived, apply the multifunctional synergistic fertilizer to the soil around the roots of the tomato plants by irrigation. The irrigation amount of the multifunctional synergistic fertilizer is 2g / m². 2 Each time, prepare fertilizer solution at a mass ratio of 1:300; at the same time, dilute the multifunctional synergistic fertilizer with water 300 times and then spray it on the leaves. The amount of fertilizer applied on the leaves is 100g / mu based on the raw materials of the fertilizer. During the survival period of tomato transplanting, apply the above-mentioned fertigation and foliar spraying methods once every 7 days until the tomatoes enter the flower bud differentiation period.

[0119] (2) Application during flower bud differentiation and flowering and fruit setting: After the tomatoes enter the flower bud differentiation period, apply the multifunctional synergistic fertilizer to the soil around the roots of the tomato plants at a rate of 3 g / m², and prepare the fertilizer solution at a mass ratio of 1:300 each time; at the same time, dilute the multifunctional synergistic fertilizer with water 300 times and then spray it on the leaves, with a foliar spraying rate of 200 g / mu based on the fertilizer raw materials, and apply phosphorus and potassium fertilizers according to the growth needs of tomatoes; before the tomatoes flower, spray the leaves again at a rate of 200 g / mu based on the fertilizer raw materials, and apply nitrogen and potassium fertilizers to meet the nutrient needs of tomatoes during flowering, fruit setting and plant growth and development; during the flower bud differentiation and flowering and fruit setting stage, apply the multifunctional synergistic fertilizer once every 10 days using the above-mentioned combination of root-side application and foliar spraying.

[0120] (3) Application during the young fruit enlargement stage: After the tomatoes enter the young fruit enlargement stage, apply the multifunctional synergistic fertilizer to the soil around the roots of the tomato plants at a rate of 4 g / m², and prepare the fertilizer solution at a mass ratio of 1:300 each time; at the same time, dilute the multifunctional synergistic fertilizer with water 300 times and then spray it on the leaves. The amount of fertilizer applied on the leaves is 200 g / mu based on the fertilizer raw materials, and nitrogen fertilizer, phosphorus fertilizer and potassium fertilizer are applied in combination according to the needs of plant growth and fruit enlargement; during the young fruit enlargement stage, apply the fertilizer once every 10 days by combining the above-mentioned root-side irrigation and foliar spraying methods.

[0121] (4) Application during flowering and fruiting period: After the tomatoes enter the flowering, fruiting and fruit coloring period, apply the multifunctional synergistic fertilizer to the soil around the roots of the tomato plants at a rate of 7.5 g / m², and prepare the fertilizer solution at a mass ratio of 1:300 each time; at the same time, dilute the multifunctional synergistic fertilizer with water 300 times and then spray it on the leaves. The amount of fertilizer applied on the leaves is 200 g / mu based on the amount of fertilizer raw materials, and nitrogen fertilizer, phosphorus fertilizer and potassium fertilizer are applied in combination according to the growth of the tomato plants and the development of the fruit; during the flowering and fruiting period, apply the fertilizer once every 15 days according to the above-mentioned root-side irrigation and foliar spraying methods.

[0122] (1) Root index: During the test, at least 5 representative plants with good growth were randomly selected for each replicate. The roots were dug up completely and washed with clean water. The number of identifiable roots with a length of not less than 1 cm was counted under a unified judgment standard, and the length of the longest root was measured.

[0123] (2) Incidence of soil-borne diseases: Fusarium wilt and root rot of tomatoes were used as evaluation indicators. No less than 50 plants were randomly surveyed in each replicate, and the disease status was determined based on typical symptoms.

[0124] Disease incidence rate (%) = Number of diseased plants / Total number of plants surveyed × 100%.

[0125] (3) Fruit setting rate determination: Select plants with uniform growth in each replicate and label them with no less than 50 normally open flowers. After the fruit setting is stable, count the number of normal young fruits formed:

[0126] Fruit set rate (%) = Number of normal fruits / Number of flowers surveyed × 100%.

[0127] (4) Average weight of a single fruit: Under the same harvest maturity, no less than 30 representative normal fruits were randomly selected for each replicate and weighed for calculation.

[0128] (5) Soluble solids in fruit: determined according to NY / T 2637-2014 "Determination of soluble solids content in fruits and vegetables by refractometer method".

[0129] (6) Soil bulk density: The bulk density of soil was determined by the ring cutter method in accordance with NY / T 1121.4-2006 Soil Testing Part 4: Determination of Soil Bulk Density.

[0130] (7) Soil water-stable aggregates: The determination was carried out in accordance with NY / T 1121.19-2008 "Soil Testing Part 19: Determination of the composition of soil water-stable macroaggregates". In this test, the proportion of water-stable aggregates with a particle size greater than 0.25 mm to the mass of the soil sample was used as the content of water-stable aggregates. The test results are shown in Table 4.

[0131] Table 4

[0132]

[0133] As shown in Table 4, the average number of roots per plant in Examples 1-5 was 55.6, an increase of approximately 32.4% compared to 42 roots per plant in Comparative Example 1; the average maximum root length was 23.1 cm, an increase of approximately 26.9% compared to Comparative Example 1. The average incidence of soil-borne diseases in Examples 1-5 was 7.86%, a decrease of approximately 13.14 percentage points compared to Comparative Example 1; the average fruit set rate was approximately 72.87%, an increase of approximately 10.87 percentage points compared to Comparative Example 1.

[0134] The average weight of a single fruit in Examples 1-5 was approximately 189.01 g, an increase of approximately 14.6% compared to Comparative Example 1; the average soluble solids content was 5.44%, an increase of approximately 0.94 percentage points compared to Comparative Example 1. The average soil bulk density in Examples 1-5 was approximately 1.254 g / cm³, a decrease of approximately 11.7% compared to Comparative Example 1; the average water-stable aggregate content was approximately 39.98%, an increase of approximately 7.98 percentage points compared to Comparative Example 1. Comparative Examples 2-5, lacking one functional microorganism respectively, showed lower performance in multiple indicators compared to Example 1 with its complete composite microbial system. Comparative Example 6, lacking active microorganisms, further reduced its overall effect; although Comparative Example 7 had the same formulation as Example 1, it used a one-time mixing of all raw materials, resulting in lower performance in root system, disease incidence, fruit set, single fruit weight, and soil properties compared to Example 1.

[0135] IV. Soil test for continuous cropping of eggplant:

[0136] Experimental conditions: Eggplant greenhouse fields that had been continuously planted for 3 years were selected as the experimental sites. A control group (fertilizers used in Comparative Examples 1-7) and an experimental group (products from Examples 1-5 of this invention) were set up. Except for the fertilizers used, the planting varieties, planting densities, water and fertilizer management, temperature management, and other cultivation management conditions were kept consistent across all groups. The specific application methods were the same as those used in the tomato experiments described above.

[0137] (1) The number of roots and the length of the main root were determined according to the aforementioned root sampling method.

[0138] (2) Soil-borne disease rate: Eggplant Verticillium wilt and Fusarium wilt were used as evaluation indicators, and the investigation method was the same as that of tomato experiment.

[0139] (3) Total yield: The total yield was calculated using the total harvest method of each plot. The total weight of the fruits that met the harvesting standard during the entire harvesting period of each repeated plot was accumulated and converted into kg / mu based on the actual plot area.

[0140] Other indicators were measured using the same method as in the tomato experiment. The test results are shown in Table 5.

[0141] Table 5

[0142]

[0143] As shown in Table 5, the average number of roots per plant in Examples 1-5 was 52.4, an increase of approximately 31.0% compared to Comparative Example 1; the average taproot length was 21.1 cm, an increase of approximately 25.6%. The average incidence of soil-borne diseases in Examples 1-5 was 8.66%, a decrease of approximately 12.84 percentage points compared to Comparative Example 1; the average fruit set rate was 70.26%, an increase of approximately 10.06 percentage points. The average weight of a single fruit was 175.68 g, an increase of 13.6% compared to Comparative Example 1; the average total yield was 4561.62 kg / mu, an increase of 20.1% compared to Comparative Example 1; the average soluble solids content was 5.13%, an increase of 0.85 percentage points compared to Comparative Example 1; the average soil bulk density was 1.266 g / cm³, a decrease of 11.5% compared to Comparative Example 1; and the average water-stable aggregate content was 37.46%, an increase of 6.96 percentage points compared to Comparative Example 1. The above results are consistent with the overall trend of the tomato experiment, indicating that the fertilizer of the present invention has shown good comprehensive application effect in different facilities for continuous cropping of solanaceous vegetables.

[0144] V. Cucumber stress resistance test:

[0145] Cucumber greenhouses that had been continuously cultivated for three years were selected for a comparative experiment using early spring greenhouse cucumbers. The control group was treated with the fertilizer prepared in the comparative proportion, while the experimental group was treated with the multifunctional synergistic fertilizer prepared according to the embodiments of this invention. Except for the type of fertilizer applied, the cucumber varieties, planting densities, cultivation methods, irrigation conditions, field management measures, and environmental conditions such as temperature and humidity were kept consistent for each treatment group. The specific application method was the same as that for tomato cultivation described above.

[0146] (1) Incidence of low temperature damage: During the experiment, the temperature inside the greenhouse was continuously recorded using a temperature and humidity recorder. A typical natural low temperature process was set: the lowest temperature inside the greenhouse was 5.8℃, the cumulative duration of temperatures below 10℃ was about 14 hours, and the cumulative duration of temperatures below 8℃ was about 6 hours. Low temperature damage was investigated 24 to 48 hours after the end of the low temperature period. All other planting and water and fertilizer management were kept consistent, and no less than 50 plants were randomly surveyed in each replicate. Plants were identified as damaged plants when they showed one or more of the following symptoms of low temperature damage: obvious leaf margin chlorosis, wilting, water-soaked spots, leaf necrosis, or damage to the growing point.

[0147] Incidence of low temperature injury (%) = Number of plants with low temperature injury / Total number of plants surveyed × 100%.

[0148] (2) Leaf SPAD value: The SPAD value was measured using a calibrated portable chlorophyll meter. At least 10 plants were randomly selected for each replicate. Fully expanded functional leaves of the same leaf position were selected from each plant. Three points were measured on each leaf, avoiding the midrib. The average value was taken as the SPAD value of the leaf.

[0149] (3) Growth period: The number of days from planting to the first harvest is calculated from the date of planting until the date when the standard for commercial harvest is first reached.

[0150] (3) Yield: The yield was calculated by continuously harvesting the entire experimental plot and then converting it to kg / mu.

[0151] (4) The commercial yield of melon strips is calculated according to the following formula:

[0152] The commercial yield of melons (%) = weight of fruits that meet the appearance and specifications requirements for commercial melons / total weight of harvested fruits × 100%.

[0153] The specific experimental results are shown in Table 6.

[0154] Table 6

[0155]

[0156] As shown in Table 6, the average incidence of low-temperature damage in Examples 1-5 was 19.21%, a decrease of 13.79 percentage points compared to Comparative Example 1. The average SPAD value of Examples 1-5 was 35.58, an increase of 16.3% compared to Comparative Example 1. The average time from transplanting to first harvest in Examples 1-5 was 26.4 days, 3.6 days earlier than Comparative Example 1. The average yield was 2889.6 kg / mu, an increase of 27.9% compared to Comparative Example 1; the average marketable fruit rate was 81.72%, an increase of 9.72 percentage points compared to Comparative Example 1. These results indicate that under natural low-temperature conditions, the fertilizer of this invention can reduce the incidence of low-temperature damage in plants and maintain a high leaf SPAD level and yield formation capacity.

[0157] VI. Low Temperature and Low Light Resistance Test of Chili Peppers:

[0158] Experimental Period: A greenhouse where chili peppers had been continuously grown for three years was selected as the experimental site. This greenhouse was prone to adverse environmental conditions such as low temperatures, insufficient sunlight, and late spring frosts in early spring. A control group and an experimental group were set up. The control group received the fertilizer prepared in the comparative proportion, while the experimental group received the multifunctional synergistic fertilizer prepared according to the embodiments of this invention. During the adverse early spring environmental period, the lowest temperature inside the greenhouse was set at 6.2℃, and the temperature was below 10℃ for a cumulative period of 12 hours. The average daily photosynthetically active radiation was approximately 35%–45% of that during the same period of normal sunny days for three consecutive days. After this period, the incidence of low-temperature damage, SPAD value, early spring yield, and marketable fruit rate were uniformly evaluated. Except for the type of fertilizer applied, all treatment groups maintained the same chili pepper varieties, planting density, irrigation methods, water and fertilizer management, temperature and humidity control, and other field cultivation management conditions. The specific application method was the same as that used for tomato cultivation.

[0159] The incidence of low-temperature damage, leaf chlorophyll content, early spring yield, and fruit marketability were measured. The methods for measuring the incidence of low-temperature damage and SPAD value were the same as in the cucumber experiment. Early spring yield was calculated as the yield of marketable fruit from transplanting to the first month of harvest. Fruit marketability was calculated as the proportion of marketable fruit weight to the total harvest weight. The experimental results are shown in Table 7.

[0160] Table 7

[0161]

[0162] As shown in Table 7, the average incidence of low-temperature damage in Examples 1-5 was 19.28%, a decrease of 13.82 percentage points compared to Comparative Example 1; the average SPAD value was 34.72, an increase of 16.1% compared to Comparative Example 1; the average early spring yield was 2342.9 kg / mu, an increase of 26.5% compared to Comparative Example 1; and the average marketable fruit rate was 80.6%, an increase of 9.4 percentage points compared to Comparative Example 1. These results indicate that under the low-temperature and low-light conditions of early spring in this experiment, crops using the fertilizer of this invention exhibited a lower incidence of low-temperature damage and higher leaf SPAD values, early spring yields, and a higher proportion of marketable fruit.

[0163] VII. Soil amendment microbial community detection:

[0164] Soils from greenhouse cultivation areas that had been continuously cropped in the same location for many years, had been treated with chemical fertilizers for a long time, and had developed root rot-related soil-borne diseases were selected as the experimental subjects. A control group and an experimental group were set up. The control group was treated with fertilizers prepared according to the respective comparative proportions, while the experimental group was treated with the multifunctional synergistic fertilizers prepared according to the embodiments of this invention. Except for the type of fertilizer applied, the crop type, planting density, irrigation method, field management, and other environmental conditions were kept consistent across all treatment groups.

[0165] During the experiment, the corresponding fertilizers were applied evenly to the soil around the crop roots via fertigation, with an application rate of 2 g / m² each time. The fertilizer solution was prepared at a mass ratio of 1:300 and applied every 6 days. Soil samples were taken after 20 consecutive days of treatment. For each treatment group, soil samples were collected from the 0–20 cm rhizosphere topsoil layer. After collection using a multi-point sampling method, the samples were thoroughly mixed, and plant debris, stones, and other impurities were removed to obtain the soil samples for testing. Each treatment was replicated three times, with each replicate sampled and tested independently.

[0166] Selective culture methods were used to detect culturable Bacillus and Bacillus-like microorganisms in soil, and preliminary identification was made based on characteristics such as colony morphology. If necessary, physiological, biochemical, or molecular methods were used for further confirmation.

[0167] Soil-borne pathogenic fungi (mainly Fusarium) were counted using PDA selective medium (containing streptomycin to inhibit bacteria) by dilution plate counting. Typical colonies were counted after incubation at 25°C for 7 days. Counting results are expressed as CFU / g dry soil. The test results are shown in Table 8.

[0168] Table 8

[0169]

[0170] As can be seen from the test results in Table 8, the number of culturable Bacillus and Bacillus-like microorganisms after treatment in Examples 1-5 was 40.8 × 10⁻⁶. 6 ~47.5×10 6 The average CFU / g dry soil was 44.78 × 10⁻⁶. 6 The CFU / g dry soil concentration was increased by 71.6% compared to Control Example 1. The number of culturable soil-borne pathogenic fungi in treatments 1-5 was 6.5 × 10⁻⁶. 5 ~9.2×10 5 CFU / g dry soil, average 7.58×10 5 The CFU / g dry soil concentration was 59.5% lower than that of Comparative Example 1. In Comparative Examples 2-5, which lacked single-function bacteria, the overall number of culturable Bacillus and Bacillus-like microorganisms was lower than in Example 1, while the overall number of culturable soil-borne pathogenic fungi was higher than in Example 1. The relevant indicators of Comparative Example 6, which lacked active microorganisms, and Comparative Example 7, which used a one-time mixing process, were also lower than those of the Examples. These results indicate that, under the conditions of this experiment, the fertilizer of the present invention can promote an increase in the number of culturable beneficial microorganisms in the rhizosphere, accompanied by a decrease in the number of culturable soil-borne pathogenic fungi, demonstrating its potential for regulating the rhizosphere microecological environment.

[0171] This document uses specific examples to illustrate the principles and implementation methods of the present invention. The descriptions of these embodiments are merely to aid in understanding the method and core ideas of the present invention, including the best mode, and to enable any person skilled in the art to practice the present invention, including manufacturing and using any device or system, and implementing any combined method. It should be noted that those skilled in the art can make various improvements and modifications to the present invention without departing from its principles, and these improvements and modifications also fall within the scope of protection of the claims. The scope of protection of this patent is defined by the claims and may include other embodiments that can be conceived by those skilled in the art. If these other embodiments have structural elements similar to those expressed in the claims, or if they include equivalent structural elements that are not substantially different from those expressed in the claims, then these other embodiments should also be included within the scope of the claims.

Claims

1. A multifunctional synergistic fertilizer based on inorganic-organic-microbial synergy, characterized in that, By weight, it includes the following components: The following components are included: 6-15 parts of yeast-derived biochemical potassium humate powder, 8-14 parts of mineral-derived potassium humate powder, 25-35 parts of potassium dihydrogen phosphate, 30-40 parts of ammonium sulfate, 0.2-0.7 parts of EDTA-chelated calcium, 1-3 parts of EDTA-chelated magnesium, 0.2-0.7 parts of EDTA-chelated zinc, 0.6-1.5 parts of EDTA-chelated iron, 0.1-0.5 parts of disodium octaborate tetrahydrate, 2-6 parts of compound microbial inoculant, 0.1-0.5 parts of γ-aminobutyric acid, 0.1-0.4 parts of D-allose, 3-7 parts of enzymatic hydrolysis extract of green algae, 0.1-0.4 parts of diethylaminoethanol succinate citrate, and 2-6 parts of polyglutamic acid. The compound microbial inoculant includes arbuscular mycorrhizal fungi inoculant, Bacillus mucilaginosus inoculant, Bacillus polymyxa inoculant, and Bacillus megaterium inoculant.

2. The multifunctional synergistic fertilizer based on inorganic-organic-microbial synergy according to claim 1, characterized in that, The total effective viable count of Bacillus mucilaginosus, Bacillus polymyxa, and Bacillus megaterium in the compound microbial agent is not less than 7.5 × 10⁸ CFU / g, and the effective propagule content in the arbuscular mycorrhizal fungi agent is ≥ 50 cells / g of agent.

3. The multifunctional synergistic fertilizer based on inorganic-organic-microbial synergy according to claim 1, characterized in that, The mass ratio of the arbuscular mycorrhizal fungal inoculant, Bacillus mucilaginosus inoculant, Bacillus polymyxa inoculant, and Bacillus megaterium inoculant is (5-8):(1-2):(1-2):(0.5-1.5).

4. The multifunctional synergistic fertilizer based on inorganic-organic-microbial synergy according to claim 1, characterized in that, The green algae enzymatic hydrolysis extract is a water-soluble or water-dispersible extract obtained from green algae raw materials after cell wall disruption and enzymatic hydrolysis.

5. A multifunctional synergistic fertilizer based on inorganic-organic-microbial synergy according to claim 1, characterized in that, The yeast-derived biochemical potassium humate powder is potassium humate powder obtained by separating, concentrating and drying yeast fermentation products; the mineral-derived potassium humate powder is potassium humate powder extracted from lignite, weathered coal or peat as raw materials.

6. A method for preparing a multifunctional synergistic fertilizer based on inorganic-organic-microbial synergy according to any one of claims 1-5, characterized in that, Includes the following steps: S1. Mix potassium dihydrogen phosphate, ammonium sulfate, calcium chelated with ethylenediaminetetraacetic acid (EDTA), magnesium chelated with EDTA, zinc chelated with EDTA, iron chelated with EDTA, and disodium octaborate tetrahydrate, pulverize and pass through an 80-mesh sieve to obtain an inorganic nutrient mixture. S2. Mix yeast-derived biochemical potassium humate powder, green algae enzymatic hydrolysis extract, γ-aminobutyric acid, D-aloose, mineral-derived potassium humate powder, diethylaminoethanol succinate citrate, and polyglutamic acid to obtain an organic functional mixture. S3. Stir and mix the inorganic nutrient mixture with the organic functional mixture to obtain a premix; S4. Add compound microbial inoculant to the premix, stir and mix, and finally package to obtain a multifunctional and efficient fertilizer.

7. The method for preparing a multifunctional synergistic fertilizer based on inorganic-organic-microbial synergy according to claim 6, characterized in that, In step S2, the mixing time is 20-30 minutes and the stirring speed is 40-60 r / min.

8. The method for preparing a multifunctional synergistic fertilizer based on inorganic-organic-microbial synergy according to claim 6, characterized in that, In step S3, the mixing time is 15-25 minutes and the stirring speed is 12-20 r / min.

9. The method for preparing a multifunctional synergistic fertilizer based on inorganic-organic-microbial synergy according to claim 6, characterized in that, In step S4, the mixing time is 8-15 minutes and the stirring speed is 8-15 r / min.

10. The method for preparing a multifunctional synergistic fertilizer based on inorganic-organic-microbial synergy according to claim 6, characterized in that, The material temperature in steps S2 to S4 is 15 to 30°C, and the highest material temperature during the preparation process does not exceed 35°C.

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