A miniature closed molecular biology experiment device

Abstract

The application provides a miniature closed molecular biology experiment device, and relates to the technical field of molecular biology experiment devices.The device comprises a shell, a first closed operation space, a second closed operation space and a third closed operation space are arranged in the shell, a partition plate is arranged between the first closed operation space, the second closed operation space and the third closed operation space, the partition plate is fixedly connected to the inner side wall of the shell, the first closed operation space, the second closed operation space and the third closed operation space are all fully-sealed structures, and an internal transmission window is formed in the partition plate.Three fully-sealed operation spaces are designed, long tube sealed operation gloves are arranged, the body of an operator is completely placed outside the device, only the hands of the operator are inserted into the operation through the gloves, direct contact between the operator and experimental samples is cut off, and the risk of infection of the operator and the risk of external transmission of pathogenic microorganisms are eliminated.

Description

Technical Field

[0001] This invention relates to the field of molecular biology experimental device technology, and in particular to a miniature closed molecular biology experimental device. Background Technology

[0002] Currently, standard molecular biology experiments, especially those involving the detection of pathogenic microorganism nucleic acid amplification (PCR), must strictly adhere to the "three zones and two channels" or similar zoning and isolation principles to prevent cross-contamination leading to false positive results and to protect the safety of operators. In traditional technical solutions, this requirement is usually achieved by constructing multiple physically separated laboratory rooms (reagent preparation room, specimen processing room, and nucleic acid amplification room), as well as corresponding buffer rooms and dedicated personnel flow channels.

[0003] For example, patent CN117345020A discloses a PCR laboratory including a corridor, a separately located molecular laboratory on one side of the corridor, and on the other side of the corridor, from left to right, a sequencing room, a product analysis room, an amplification room, a sample processing room, and a reagent preparation room. A sample receiving area and a UPS room are separately located next to the reagent preparation room. A first transfer window is provided in the wall between the sample receiving area and the outside; a second transfer window is provided in the wall between the reagent preparation room and the sample processing room; a third transfer window is provided in the wall between the sample processing room and the amplification room; a fourth transfer window is provided in the wall between the amplification room and the product analysis room; and a fifth transfer window is provided in the wall between the product analysis room and the sequencing room. This patent illustrates the structural layout of a conventional laboratory.

[0004] This traditional construction and operation model has the following drawbacks: 1. Laboratory personnel must wear multiple layers of protective equipment (protective suits, masks, goggles, etc.) and enter and exit each experimental area sequentially through buffer zones. Each experimental stage often requires changing some or all of the protective equipment, making the entire process time-consuming and labor-intensive, thus limiting sample throughput and detection speed.

[0005] Personnel directly exposed to high-risk experimental areas pose a safety risk of infection by pathogens.

[0006] To meet the zoning requirements, dedicated spaces of hundreds of square meters are needed, along with complex air systems to maintain pressure gradients and directional airflow in each zone. This not only results in extremely high civil engineering and facility purchase costs, but also high energy consumption and maintenance costs during daily operation, making it difficult to promote in scenarios such as primary healthcare units and mobile testing platforms.

[0007] To address this, a miniature closed molecular biology experimental device is proposed. Summary of the Invention

[0008] In view of this, the present invention provides a miniature closed molecular biology experimental device to solve or alleviate one of the technical problems existing in the prior art, and at least provides a beneficial alternative.

[0009] The technical solution of this invention is implemented as follows: A miniature closed molecular biology experimental device includes a shell. The shell contains a first closed operating space, a second closed operating space, and a third closed operating space. A partition is provided between the first, second, and third closed operating spaces. The partition is fixedly connected to the inner wall of the shell. The first, second, and third closed operating spaces are all fully sealed structures. An internal transfer window is provided on the partition. Electric guide rails are symmetrically installed on both sides of the internal transfer window. A sealing plate is installed on the electric guide rails. A first ultraviolet lamp plate is installed on the top of the inner wall of the internal transfer window. Three observation windows are evenly distributed on the surface of the shell. Three sets of long-tube sealed operating gloves are evenly fixedly connected to the surface of the shell. Three sets of input and output components are evenly distributed on the surface of the shell. A high-temperature sterilization component is provided on the shell.

[0010] Furthermore: the input / output assembly includes an input window, an output window, and a sealing door; the surface of the housing is uniformly provided with input and output windows, and the front surface of the input and output windows is provided with a sealing door.

[0011] Furthermore, a first baffle and a second baffle are symmetrically fixedly connected to one side of the input window and the output window, and a back plate is fixedly connected to one side of the first baffle and the second baffle.

[0012] Furthermore: a motor is mounted on the top of the back plate, the output shaft of the motor is fixedly connected to one end of a threaded rod, and a slider is threadedly connected to the outer side wall of the threaded rod.

[0013] Furthermore: a lifting platform is fixedly connected to the surface of the slider, and a third ultraviolet lamp plate is installed on the top of the inner wall of the lifting platform.

[0014] Furthermore: the high-temperature disinfection component includes a circulating air duct, a circulating fan, and a heater; The first enclosed operating space, the second enclosed operating space, and the third enclosed operating space are all connected to circulating air ducts, and circulating fans and heaters are installed on the three circulating air ducts.

[0015] Furthermore, the heater is located inside the housing.

[0016] Furthermore, an operation panel is mounted on the surface of the housing.

[0017] Furthermore, a second ultraviolet lamp plate is installed on one side of the partition.

[0018] Furthermore, three support legs are evenly and fixedly connected to the bottom of the housing.

[0019] The embodiments of the present invention have the following advantages due to the adoption of the above technical solutions: I. This invention, through the design of three fully sealed operating spaces and the provision of long, sealed operating gloves, ensures that the operator's body is completely outside the device, with only their hands inserted through the gloves. This cuts off direct contact between the operator and the experimental samples, eliminating the risk of personnel infection and the spread of pathogenic microorganisms.

[0020] Second, this invention integrates three functional spaces into a single shell, resulting in a compact structure. Compared to the traditional method of having three experimental rooms plus a buffer room, this device occupies a smaller area and can be directly deployed in a regular room without the need for complex civil engineering modifications and area separation, thus reducing the construction threshold and cost.

[0021] Third, thanks to the integrated sealed design of the three functional spaces, the experimenters do not need to wear and repeatedly change protective clothing, nor do they need to pass through the buffer room. They can complete the entire experimental process with gloves. Materials are transferred through the input and output components with disinfection function, which improves the sample processing capacity per unit time.

[0022] Fourth, this invention utilizes a lifting platform to realize the transfer function of items. When items are brought in or sent out, they are lifted and lowered by the lifting platform and the transfer area is disinfected with ultraviolet light. This is equivalent to setting up a miniature clean buffer space at the window, which isolates external pollution from entering the operating space and prevents internal risks from spreading outward, thus enhancing the biosafety performance of the device.

[0023] V. After the experiment is completed, the interior can be disinfected using a high-temperature sterilization component, ensuring a clean environment and accurate results for the next experiment. The circulating fans in each space can be started independently to achieve uniform high-temperature sterilization of their respective spaces. The three spaces share a single heater, reducing the manufacturing material costs, overall weight, and operating energy consumption of the device, resulting in a compact structure.

[0024] The above overview is for illustrative purposes only and is not intended to be limiting in any way. In addition to the illustrative aspects, embodiments, and features described above, further aspects, embodiments, and features of the invention will become readily apparent from the accompanying drawings and the following detailed description. Attached Figure Description

[0025] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0026] Figure 1 This is a structural diagram of the present invention from one perspective; Figure 2 This is a structural diagram from another perspective of the present invention; Figure 3 This is a diagram showing the distribution of the enclosed operating space of the present invention; Figure 4 This is a diagram of the internal structure of the housing of the present invention; Figure 5 This is a structural diagram of the lifting platform of the present invention; Figure 6 This is a structural diagram of the backplate of the present invention; Figure 7 This is a diagram of the internal structure of the internal transfer window of the present invention.

[0027] Reference numerals: 11. Housing; 12. First enclosed operating space; 13. Second enclosed operating space; 14. Third enclosed operating space; 15. Partition; 16. Internal transfer window; 17. Electric guide rail; 18. Sealing plate; 19. Observation window; 20. Long sealed operating glove; 21. Operating panel; 22. Second ultraviolet lamp panel; 23. Support leg; 24. First ultraviolet lamp panel; 30. Input / output assembly; 31. Input window; 32. Output window; 33. Lifting platform; 34. Third ultraviolet lamp panel; 35. First baffle; 36. Second baffle; 37. Back plate; 38. Motor; 39. Threaded rod; 310. Slider; 311. Sealed door; 40. High-temperature disinfection assembly; 41. Circulating air duct; 42. Circulating fan; 43. Heater. Detailed Implementation

[0028] In the following description, only certain exemplary embodiments are briefly described. As those skilled in the art will recognize, the described embodiments can be modified in various ways without departing from the spirit or scope of the invention. Therefore, the drawings and description are considered to be exemplary in nature and not restrictive.

[0029] The embodiments of the present invention will now be described in detail with reference to the accompanying drawings.

[0030] Example 1 like Figures 1 to 6 As shown, this embodiment of the invention provides a miniature closed molecular biology experimental device.

[0031] The main structure of this device includes a shell 11, which serves as the load-bearing and protective outer shell for the entire device. Inside the shell 11, a unique spatial layout separates three functionally independent operating areas: a first enclosed operating space 12, a second enclosed operating space 13, and a third enclosed operating space 14. These three spaces are not directly connected but are physically separated by partitions 15 fixedly connected to the inner wall of the shell 11. To ensure biosafety during operation, each operating space is designed as a fully sealed structure, thereby creating independent, closed microenvironments within the device.

[0032] To ensure the safe transfer of materials between the three sealed spaces without compromising their airtightness, an internal transfer window 16 is provided on the partition 15. This window establishes a controllable material exchange channel between the different spaces. Electric guide rails 17 are symmetrically installed on both sides of the internal transfer window 16, with sealing plates 18 mounted on the rails. A first ultraviolet lamp plate 24 is installed on the top of the inner wall of the internal transfer window 16. The sealing plates 18 on both sides are configured not to open simultaneously. When items need to be transferred, the electric guide rail 17 drives one side of the sealing plate 18 to slide, opening the transfer window to allow the item to be transferred. After the transfer is complete, the sealing plate 18 on the transfer side closes and tightens, and the item is disinfected by the first ultraviolet lamp plate 24. After disinfection, the sealing plate 18 on the other side opens, and the operator removes the item. The operator can complete the sealed and contamination-free transfer of reagents, specimens, and other materials between the various operating spaces without directly contacting the transfer process.

[0033] To facilitate clear observation of the experimental process and conditions inside each space from the outside, three observation windows 19 are evenly opened on the surface of the shell 11. Each observation window 19 corresponds to a closed operating space. Through the observation window 19 made of transparent material, personnel can monitor the experimental dynamics inside the operating space in real time.

[0034] Physical isolation between operators and experimental samples is crucial for achieving biosafety. To this end, three sets of long, sealed operating gloves 20 are uniformly and fixedly attached to the surface of the housing 11, each corresponding to a specific operating space. The gloves are tightly sealed to the housing 11, allowing operators to enter the space and perform all experimental operations simply by placing their hands inside the gloves while standing outside the device. Throughout the entire operation, the operator's body remains outside the device, without direct contact with any internal samples or the surrounding environment, thus eliminating the risk of infection and human contamination at its source.

[0035] The device is also equipped with three sets of precisely structured input and output components 30, which are evenly distributed on the surface of the shell 11 to realize the sealed input of experimental items and the sealed output of waste, overcoming the difficulty of exchanging items in a closed space.

[0036] In this embodiment, the input / output assembly 30 is specifically configured as follows: it includes an input window 31 and an output window 32, which are respectively opened on the surface of the housing 11. The input window 31 is used to input clean items such as reagents and consumables into the space, while the output window 32 is used to output experimental products and waste from the space to the outside. The front surface of both windows is equipped with a sealing door 311, which remains closed in the non-transfer state to maintain the airtightness of the space.

[0037] More specifically, in order to establish a clean buffer zone during the transfer of goods and prevent external contaminants from directly entering the operating space or internal contaminants from spreading outward, a set of structural components, namely a first baffle 35 and a second baffle 36, are symmetrically fixedly connected to one side of the inlet window 31 and the outlet window 32. A back plate 37 is also fixedly connected to the same side of these two baffles. In this way, the two side plates, the back plate 37, the housing 11, and the sealing door 311 together form a relatively independent, partially semi-enclosed area, constituting the buffer zone for the transfer operation.

[0038] This buffer zone integrates an automated item conveying mechanism. Specifically, a motor 38, providing power, is mounted at the top of the back plate 37. The output shaft of the motor 38 faces downwards, and its end is fixedly connected to a vertically arranged threaded rod 39. A slider 310 is threadedly connected to the outer wall of the threaded rod 39. When the motor 38 receives a command to rotate forward or reverse, its output shaft drives the threaded rod 39 to rotate synchronously. Through threaded transmission, this drives the slider 310 to perform precise upward or downward linear motion along the axial direction of the threaded rod 39.

[0039] A lifting platform 33 for carrying items is fixedly connected to the surface of the slider 310. The lifting platform 33 serves as a transfer platform for item transfer, and its complete workflow is as follows: When an item is being transferred in, the operator first opens the outermost sealed door 311, places the item on the lowered lifting platform 33, and then closes the sealed door 311. At this time, the motor 38 starts, driving the lifting platform 33 to rise via the threaded rod 39 and the slider 310, raising the item to within the operator's reach. The operator uses long sealed operating gloves 20 to remove the item from the lifting platform 33 into the operating space. After the item is removed, the lifting platform 33 descends to its original position, and the third ultraviolet lamp panel 34 installed on the top of its inner wall illuminates, thoroughly disinfecting the lifting platform 33 and the buffer zone, preparing for the next transfer. The process for transferring items is the reverse: the internal operator places waste on the higher-positioned lifting platform 33, the lifting platform 33 descends to its lower position, and external personnel can open the sealed door 311 to remove it.

[0040] This device is also equipped with a high-temperature sterilization component 40, which is used to thoroughly disinfect each sealed operating space after the experiment.

[0041] Specifically, the high-temperature disinfection component 40 is composed and connected as follows: it includes three parts: a circulating air duct 41, a circulating fan 42, and a heater 43. Each of the first enclosed operating space 12, the second enclosed operating space 13, and the third enclosed operating space 14 is independently connected to a circulating air duct 41, and all three circulating air ducts 41 are connected to the heater 43. At the same time, an independent circulating fan 42 is installed on the passage of each circulating air duct 41, ensuring that the air in each space can circulate in its own independent closed loop without mixing with the air in other spaces.

[0042] More specifically, in terms of spatial layout, for a compact structure and concentrated heat utilization, the heater 43 is located in the central position inside the housing 11. When the high-temperature disinfection program is activated, the heater 43 generates high temperature, and each circulating fan 42 operates independently, drawing air from its own space into the circulating air duct 41, which then flows through the heater 43 for further heating before being returned to the space, forming a forced circulation of high-temperature air until the temperature in the space uniformly rises to the predetermined disinfection temperature and is maintained. This design has three advantages: First, the airflow circulation in each space is completely independent, eliminating the risk of contamination diffusion caused by aerosol pollutants such as amplification products remaining in different spaces during the disinfection process through cross-flow, ensuring that each space is independent and does not interfere with each other; Second, the three spaces share one heater 43, which reduces the number of parts, manufacturing costs, and overall energy consumption compared to setting separate heaters for each space, resulting in a more compact and economical structure; Third, the forced hot air circulation heating ensures uniform temperature rise throughout the space, eliminating disinfection dead zones.

[0043] In terms of eliminating safety hazards, this high-temperature sterilization component 40 employs multiple structural and control designs to mitigate various safety risks during laboratory sterilization, comprehensively ensuring the safety of experimental equipment and operations. Firstly, relying on the independent closed-loop airflow circulation structure of each space, it eliminates potential sterilization safety hazards. The airflow in the three operating spaces is completely independent and not interconnected, fundamentally preventing the cross-diffusion and cross-contamination of amplification products and pathogenic microbial aerosols remaining in different experimental areas. This effectively prevents sample cross-contamination and distorted experimental results, while also preventing the leakage of harmful aerosols, ensuring a clean laboratory environment and the safety of experimental personnel. Secondly, the component is equipped with independent start-stop interlocks and temperature control protection mechanisms to eliminate safety hazards during high-temperature operation. The high-temperature disinfection program can only be activated when all sealed operating spaces are completely closed and the doors are locked, preventing hot air leakage that could burn personnel or damage surrounding equipment. It also features built-in over-temperature power-off protection and overheat fuse protection devices. When the space temperature exceeds a preset safety threshold, the heater immediately shuts down, while the circulating fan continues to dissipate heat, effectively avoiding safety malfunctions such as high-temperature overload, equipment burnout, and overheating deformation. Thirdly, the independent air duct design avoids airflow turbulence and negative pressure backflow problems, preventing the backflow of pollutants during disinfection and eliminating the safety hazards of residual pollutant accumulation and secondary pollution, ensuring that the disinfection environment of each operating space is independent, safe, and controllable.

[0044] In terms of energy-saving optimization, this component reduces the energy consumption of the disinfection equipment through three major designs: shared heat source, optimized heat loss, and precise temperature control. This solves the problems of high power consumption, large power consumption, and high operating costs of traditional multi-space disinfection equipment. First, the three operating spaces share a central heater 43, replacing the traditional structure where each space has its own independent heater. This significantly reduces the number of heating components, lowers standby power consumption and hardware wear, and reduces power consumption from a hardware perspective, effectively reducing equipment manufacturing costs and long-term operating energy consumption. Second, the heater is located in the center of the casing, combined with a short-stroke circulating air duct layout, shortening the high-temperature airflow transmission path, reducing heat loss in the ductwork, improving heat utilization, and avoiding ineffective heat loss. Under the same disinfection temperature and duration, the workload of the heater can be significantly reduced, thus reducing power consumption. Finally, the equipment is equipped with an intelligent constant temperature control system. During the heating phase, it operates at full power to quickly reach the target temperature. After the temperature reaches the preset disinfection threshold, the heater automatically operates at a constant temperature with variable frequency, supplementing only a small amount of heat to maintain the disinfection temperature, thus avoiding the energy waste caused by continuous full-power heating. At the same time, the disinfection operation can be started and stopped independently in a single space, without the need for simultaneous operation in multiple spaces. It can perform targeted disinfection according to the actual experimental usage, avoiding the ineffective power consumption caused by full-area disinfection, and significantly reducing the daily power consumption of the equipment.

[0045] In addition, conventional auxiliary disinfection methods are also integrated into the device. Specifically, a second ultraviolet lamp plate 22 is installed on one side of the partition 15, which can provide continuous ultraviolet irradiation antibacterial ability to the adjacent operating space, forming a dual disinfection complement of high temperature and ultraviolet light, ensuring the continuous cleanliness of the internal environment of the device.

[0046] In terms of operation and control, an operation panel 21 is installed on the surface of the housing 11. The operation panel 21 is electrically connected to all electrical components inside the device, such as the motor 38, electric guide rail 17, circulating fan 42, heater 43, and ultraviolet lamp panel. Through the operation panel 21, the experimenter can centrally control all functions, such as opening and closing the internal transfer window, raising and lowering the lifting platform 33, and starting and stopping the high-temperature sterilization program, either with one button or in steps. The operation is convenient.

[0047] Finally, to ensure the stable placement of the device, three support legs 23 are evenly and fixedly connected to the bottom of the housing 11, providing stable and reliable support for the entire device.

[0048] In operation, before conducting molecular biology experiments, the high-temperature sterilization component 40 is activated via the control panel 21 to sterilize the interior of the device. The heater 43 generates high-temperature air, and the circulating fans 42 of each of the three enclosed operating spaces operate independently, drawing air from that space through the circulating duct 41, heating it in the heater 43, and then returning it to its respective space, forming an independent forced circulation of high-temperature air for comprehensive sterilization of each operating space. Simultaneously, the second ultraviolet lamps 22 on each partition 15 can also be activated, forming a dual sterilization mechanism combining high temperature and ultraviolet light to ensure the interior of the device is clean and sterile before the experiment begins.

[0049] After the disinfection procedure is completed, the device enters the experimental preparation stage. The operator stands outside the housing 11, facing the device. First, the raw materials and consumables required for reagent preparation are introduced through the input / output assembly 30 corresponding to the first enclosed operating space 12. The specific operation is as follows: the operator opens the sealed door 311 outside the input window 31 of this space, places the items on the lowered lifting platform 33, and closes the sealed door 311; then, the operator controls the motor 38 to start via the control panel 21, driving the threaded rod 39 to rotate, driving the slider 310 and the lifting platform 33 to rise to the input window 31; the operator puts both hands into the corresponding long sealed operating gloves 20, and uses the gloves to take the items from the lifting platform 33 into the first enclosed operating space 12; after taking out the items, the lifting platform 33 descends and resets, and the third ultraviolet lamp panel 34 is lit to disinfect the lifting platform 33 and the buffer zone. Following the same operating procedure, the specimens to be processed, nucleic acid extraction reagents, and consumables are introduced into the second enclosed operating space 13 through the input / output assembly 30; the reaction reagents and detection consumables required for nucleic acid amplification are introduced into the third enclosed operating space 14.

[0050] After all materials in each space are prepared, the experiment officially begins. The operator, wearing long, sealed operating gloves 20, prepares the reaction reagents in the first enclosed operating space 12. After preparation, a portion of the reagents needs to be transferred to the second enclosed operating space 13. At this time, the operator uses the control panel 21 to control the movement of the electric guide rail 17 on the partition 15 between the first and second enclosed operating spaces 12 and 13, driving the sealing plate 18 to slide open the internal transfer window 16. After the items are placed inside, the internal transfer window 16 closes, and the items are disinfected by the first ultraviolet lamp plate 24. After disinfection, the internal transfer window 16 reopens, and the operator, wearing gloves, passes the prepared reagents through the window into the second enclosed operating space 13. After the transfer is complete, the electric guide rail 17 drives the sealing plate 18 to close, restoring the sealing state of the partition 15.

[0051] Operators perform sample processing and nucleic acid extraction within the second enclosed operating space 13. After completing steps such as sample lysis, nucleic acid extraction, and purification while wearing gloves, the extracted nucleic acid is mixed with the prepared reaction reagents and added to the sample. After addition, the sealed reaction tube is transferred to the third enclosed operating space 14 through the internal transfer window 16 between the second and third enclosed operating spaces 13, and then the transfer window is closed.

[0052] Finally, the operator completes nucleic acid amplification and result analysis within the third enclosed operating space 14. Wearing gloves, the reaction tube is placed into the amplification detection device, the amplification program is started, and the amplification process is monitored in real time through the observation window 19 to obtain and interpret the detection results.

[0053] After the experiment is completed, the waste generated during the experiment must be safely removed. Taking the second enclosed operating space 13 as an example, the operator, wearing gloves, places the waste onto the lifting platform 33 at the exit window 32 of the space. The operator controls the motor 38 to reverse through the control panel 21, causing the lifting platform 33 to descend. At the same time, the third ultraviolet lamp panel 34 is lit to disinfect the waste and its surroundings after it has descended. After disinfection, external personnel open the sealed door 311 to remove the waste and dispose of it properly.

[0054] After all experiments and waste disposal are completed, the high-temperature sterilization unit 40 is restarted to perform comprehensive terminal sterilization on the first enclosed operating space 12, the second enclosed operating space 13, and the third enclosed operating space 14, in preparation for the next experiment.

[0055] Throughout the experiment, the operator's body remained outside the shell 11, and the operator only entered the enclosed operating space through long sealed operating gloves 20. There was no need to wear protective clothing, change any protective equipment, or enter the experimental area, which ensured the safety of personnel from the source and eliminated cross-contamination.

[0056] Example 2 This embodiment uses the miniature closed molecular biology experimental device described in this invention to perform real-time fluorescence quantitative RT-PCR detection of influenza A virus on a batch of clinical pharyngeal swab specimens as an example to explain the specific operation process and parameters of this device in detail.

[0057] I. Disinfection before experiment The disinfection program on the control panel 21 is activated. The heater 43 heats the circulating air to 180°C. The circulating fans 42 corresponding to the three enclosed operating spaces operate independently, allowing the high-temperature air in each space to circulate at a rate of 5m. 3 A closed-loop airflow of / min is maintained to keep the temperature in the space uniformly above 160℃ for 30 minutes. Simultaneously, the second ultraviolet lamp panel 22 on each partition 15 automatically turns on, providing a radiation illuminance of 90μW / cm². 2 The space is then disinfected using ultraviolet light. After the disinfection process is complete, the device is allowed to cool naturally to room temperature and then enters standby mode.

[0058] II. Transfer of Experimental Materials First enclosed operating space 12 (reagent preparation area): The operator presses the corresponding space's input button on the operating panel 21, and the motor 38 drives the lifting platform 33 to descend to its lowest position. The operator opens the sealed door 311 outside the input window 31 and places the following items on the lifting platform 33: nuclease-free water, RT-PCR reaction buffer, specific primer and probe mixture, enzyme mixture, positive control template, negative control template, 1.5mL sterile centrifuge tubes, and pipette tip boxes, etc. After closing the sealed door 311, the lifting platform 33 automatically rises, and the operator puts both hands into the long sealed operating gloves 20 to remove all items into the first enclosed operating space 12. The lifting platform 33 descends and resets, and the third ultraviolet lamp panel 34 on its inner wall top illuminates, disinfecting the lifting platform 33 and its buffer zone with ultraviolet light for 5 minutes, preparing for subsequent transfers.

[0059] Second enclosed operating space 13 (specimen processing and nucleic acid extraction area): Following the same operating procedures, clinical pharyngeal swab specimen tubes, nucleic acid extraction kits (including lysis buffer, washing buffer, elution buffer, magnetic beads, etc.), sterile centrifuge tubes and pipette tips are transferred into this space.

[0060] The third enclosed operating space 14 (nucleic acid amplification and result analysis area): Consumables such as PCR eight-tube strips, microcentrifuge tubes, and micropipette tips are transferred to this space.

[0061] III. Reagent Preparation With both hands inside the first enclosed operating space 12, the operator uses gloves to operate a micropipette and sequentially adds nuclease-free water, RT-PCR reaction buffer, primer-probe mixture, and enzyme mixture to a sterile centrifuge tube. The mixture is then mixed to prepare the RT-PCR reaction solution. The prepared reaction solution is then dispensed into multiple sterile centrifuge tubes and capped for later use.

[0062] IV. Specimen Processing and Nucleic Acid Extraction The operator moves their hands inside the gloves in the second enclosed operating space 13. The pharyngeal swab sample tube is taken, thoroughly shaken to mix, and 200 μL of the sample solution is added to a centrifuge tube containing lysis buffer. Following the instructions of the nucleic acid extraction kit, the operator sequentially completes the lysis, magnetic bead adsorption, washing, and elution operations through the gloves, ultimately obtaining 50 μL of purified viral RNA eluent.

[0063] V. Sampling and Transfer In the second enclosed operating space 13, the operator uses a pipette to draw 5 μL of the above RNA elution buffer and adds it to the previously prepared RT-PCR reaction solution. The tube is capped and mixed thoroughly. After the reaction tube with the sample added is labeled, it is placed in a sealed transfer container.

[0064] Subsequently, the internal transfer window 16 between the second closed operating space 13 and the third closed operating space 14 is opened via the control panel 21. The electric guide rail 17 drives the sealing plate 18 to slide open. After the item is placed in, the internal transfer window 16 is closed. The item is disinfected by the first ultraviolet lamp plate 24. After the disinfection is completed, the internal transfer window 16 is opened again. The operator, wearing gloves, passes the transfer container from the window into the third closed operating space 14. Then, the internal transfer window 16 is closed, and the seal of the partition 15 is restored.

[0065] VI. Nucleic Acid Amplification and Result Analysis The operator moves both hands inside the gloves in the third enclosed operating space 14, opens the transfer container, takes out the reaction tubes with samples added, and places them one by one into the sample slots of the real-time quantitative PCR instrument. The PCR instrument is controlled via the operation panel 21 to run according to the following program: reverse transcription at 50℃ for 30 minutes; pre-denaturation at 95℃ for 2 minutes, followed by 40 cycles, with fluorescence signals from the FAM channel collected during the annealing and extension phase.

[0066] During the amplification process, the operator observes the amplification curve displayed on the PCR instrument screen in real time through the observation window 19 in this space. After the reaction, the results are interpreted based on the Ct value and the shape of the amplification curve: a positive control with a Ct value ≤ 30 and a typical S-shaped amplification curve, and a negative control with no Ct value, indicate that the experiment is effective; a test sample with a Ct value ≤ 38 and a good curve is judged as positive; a Ct value > 38 or no peak is judged as negative.

[0067] VII. Waste Disposal and Terminal Disinfection After the experiment, the operators transferred the used specimen tubes, centrifuge tubes containing waste lysis buffer, and other potentially contaminated waste from the second enclosed operating space 13 through the transfer window 32. Specifically, the waste was placed on the lifting platform 33 inside the transfer window 32, the transfer button was pressed, the lifting platform 33 descended, and the third ultraviolet lamp panel 34 was activated for 10 minutes of disinfection. Afterward, external personnel opened the sealed door 311 to remove the waste for autoclaving. Other waste consumables from the three spaces were transferred out in the same manner.

[0068] Finally, the high-temperature sterilization component 40 was restarted, and 180°C hot air was circulated and sterilized in each space for 30 minutes to ensure that the inside of the device was thoroughly cleaned and to prepare for the next batch of experiments.

[0069] The above description is merely a specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any person skilled in the art can easily conceive of various variations or substitutions within the technical scope disclosed in the present invention, and these should all be included within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be determined by the scope of the claims.

Claims

1. A miniature closed molecular biology experimental device, comprising a shell (11), characterized in that: The housing (11) is provided with a first closed operating space (12), a second closed operating space (13), and a third closed operating space (14). A partition (15) is provided between the first closed operating space (12), the second closed operating space (13), and the third closed operating space (14). The partition (15) is fixedly connected to the inner wall of the housing (11). The first closed operating space (12), the second closed operating space (13), and the third closed operating space (14) are all fully sealed structures. An internal transfer window (1) is provided on the partition (15). 6) Electric guide rails (17) are symmetrically installed on both sides of the internal transfer window (16). A sealing plate (18) is installed on the electric guide rail (17). A first ultraviolet lamp plate (24) is installed on the top of the inner wall of the internal transfer window (16). Three observation windows (19) are evenly arranged on the surface of the housing (11). Three sets of long-tube sealed operating gloves (20) are evenly fixedly connected to the surface of the housing (11). Three sets of inlet and outlet components (30) are evenly arranged on the surface of the housing (11). A high-temperature sterilization component (40) is provided on the housing (11).

2. The miniature closed molecular biology experimental device according to claim 1, characterized in that: The input / output assembly (30) includes an input window (31), an output window (32), and a sealing door (311); the surface of the housing (11) is uniformly provided with the input window (31) and the output window (32), and the front surface of the input window (31) and the output window (32) is provided with a sealing door (311).

3. The miniature closed molecular biology experimental device according to claim 2, characterized in that: The input window (31) and output window (32) are symmetrically fixedly connected to one side of a first baffle (35) and a second baffle (36), and a back plate (37) is fixedly connected to one side of the first baffle (35) and the second baffle (36).

4. The miniature closed molecular biology experimental device according to claim 3, characterized in that: A motor (38) is mounted on the top of the back plate (37). The output shaft of the motor (38) is fixedly connected to one end of a threaded rod (39). A slider (310) is threadedly connected to the outer wall of the threaded rod (39).

5. A miniature closed molecular biology experimental device according to claim 4, characterized in that: The surface of the slider (310) is fixedly connected to a lifting platform (33), and a third ultraviolet lamp plate (34) is installed on the top of the inner wall of the lifting platform (33).

6. The miniature closed molecular biology experimental device according to claim 1, characterized in that: The high-temperature disinfection component (40) includes a circulating air duct (41), a circulating fan (42), and a heater (43). The first enclosed operating space (12), the second enclosed operating space (13) and the third enclosed operating space (14) are all connected to a circulating air duct (41), and a circulating fan (42) and a heater (43) are installed on the three circulating air ducts (41).

7. A miniature closed molecular biology experimental device according to claim 6, characterized in that: The heater (43) is located inside the housing (11).

8. A miniature closed molecular biology experimental device according to claim 1, characterized in that: An operation panel (21) is mounted on the surface of the housing (11).

9. A miniature closed molecular biology experimental device according to claim 1, characterized in that: A second ultraviolet lamp plate (22) is installed on one side of the partition (15).

10. A miniature closed molecular biology experimental device according to claim 1, characterized in that: The bottom of the housing (11) is uniformly and fixedly connected with three support legs (23).

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