Peripheral blood autologous immune cell culture device and preparation method

Abstract

The application discloses a kind of peripheral blood autoimmune cell culture devices and preparation method, it is related to immune cell culture equipment application technical field, present and propose the following scheme, including organism and hinged in the sealed door of organism outside, further include: motor frame, the motor frame is installed in the inside of organism, drive mechanism is equipped on the motor frame;Control mechanism, the control mechanism is installed in the inside of organism, one side of the control mechanism is connected with drive mechanism, the control mechanism includes with the fixed connection of the inside of organism push rod motor, with the fixed connection of push rod motor output shaft traction plate and the linkage of bearing and traction plate rotation Linking rod.The immune cell sample in the placing tray can be sealed and cultivated by the application, and the problem of contamination caused by the immune cells being taken out for viewing is effectively solved by adjusting the height of the placing tray, and the culture effect and viewing effect of the immune cells are effectively improved.

Description

Technical Field

[0001] This invention relates to the field of immune cell culture equipment application technology, and in particular to a culture device and preparation method for peripheral blood autologous immune cells. Background Technology

[0002] Immune cells are cells that participate in or are related to the immune response, including lymphocytes, dendritic cells, monocytes / macrophages, granulocytes, mast cells, etc. Various immune cells play important roles in the human body. In some hospitals and research institutes, in order to better understand the growth and reproduction process of immune cells and their other functions, other culture devices are used for cultivation. Therefore, an immune cell culture device is highly sought after. However, after analysis, it was found that immune cells need a suitable temperature during cultivation, and existing immune cell culture devices cannot automatically adjust the temperature required for the growth and reproduction of immune cells according to the environment.

[0003] Furthermore, during immune cell culture, staff need to regularly observe the growth and reproduction of immune cells. When culturing immune cells using existing immune cell culture devices, the device needs to be opened and the culture dish removed for observation. At this time, a large number of bacteria and viruses may take the opportunity to enter the immune cell culture device and contaminate the immune cell culture environment. Therefore, we propose a culture device and preparation method for peripheral blood autologous immune cells. Summary of the Invention

[0004] This invention provides a culture device and preparation method for peripheral blood autologous immune cells to address the aforementioned shortcomings in the prior art.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] A peripheral blood autologous immune cell culture device includes a body and a sealed door hinged to the outside of the body, and further includes:

[0007] A motor frame is installed inside the machine body, and a drive mechanism is provided on the motor frame;

[0008] The control mechanism is installed inside the machine body. One side of the control mechanism is connected to the drive mechanism. The control mechanism includes a push rod motor fixedly connected to the inside of the machine body, a traction plate fixedly connected to the output shaft of the push rod motor, and a connecting rod rotatably connected to the traction plate through a bearing. The drive mechanism drives the connecting rod to rotate.

[0009] A pushing mechanism is installed inside the machine body. A first transmission mechanism is provided between one side of the pushing mechanism and the control mechanism. The pushing mechanism includes a first threaded rod rotatably connected to the inside of the machine body, a moving block threadedly connected to the first threaded rod, and a connecting plate fixedly connected to one side of the moving block. The rotation of the connecting rod drives the rotation of the first threaded rod through the first transmission mechanism. The rotation of the first threaded rod drives the moving block to move. The movement of the moving block synchronously drives the connecting plate to move.

[0010] A height adjustment mechanism is installed inside the machine body. A second transmission mechanism is provided between one side of the height adjustment mechanism and the control mechanism. The height adjustment mechanism includes a second threaded rod rotatably connected to the moving block, an internal threaded sleeve threadedly connected to the second threaded rod, and a placement plate fixedly connected to the top of the internal threaded sleeve. The rotation of the linkage rod drives the second threaded rod to rotate through the second transmission mechanism. The rotation of the second threaded rod drives the internal threaded sleeve to move, and simultaneously drives the placement plate to move.

[0011] Furthermore, the drive mechanism includes a drive motor fixedly connected to the motor frame, and the output shaft of the drive motor is fixedly connected to a first gear disk.

[0012] Furthermore, the control mechanism also includes a gear column fixedly connected to one end of the linkage rod, one side of the gear column meshing with the first gear disk for transmission, and a first actuating plate and a second actuating plate symmetrically fixed on the linkage rod.

[0013] Furthermore, the pushing mechanism also includes a guide rod fixedly connected to the inside of the machine body. A guide plate is fixedly connected to the connecting plate at the corresponding guide rod. A guide hole is opened on the guide plate. The guide rod moves through the inside of the guide hole. Two slide rods are symmetrically fixedly connected inside the machine body. The same slide plate is movably sleeved on the two slide rods. A first through hole is opened at the corresponding internal threaded sleeve of the slide plate. The internal threaded sleeve is movably sleeved inside the first through hole.

[0014] Furthermore, the height adjustment mechanism also includes a support frame fixedly connected to the bottom of the connecting plate. A worm gear is rotatably connected to one side of the support frame, and a worm wheel is fixedly connected to the second threaded rod corresponding to the worm gear. The worm wheel meshes with the worm gear for transmission. A limit slider is fixedly connected to one side of the worm gear. A second transmission sleeve is rotatably connected inside the body. A moving groove is opened on the second transmission sleeve corresponding to the limit slider. The worm gear is movably sleeved inside the second transmission sleeve, and the limit slider is movably sleeved inside the moving groove. A balance plate is fixedly connected below the internal threaded sleeve. Two limit rods are fixedly connected between the balance plate and the placement plate. A second through hole is opened on the slide corresponding to each of the two limit rods, and the two limit rods are movably sleeved inside the corresponding second through holes.

[0015] Furthermore, the first transmission mechanism includes a first transmission sleeve rotatably connected to the inner bottom end of the machine body. A first driven plate is fixedly connected to one end of the first transmission sleeve. One side of the first driven plate abuts against a first actuating plate. A second gear disk is fixedly connected to the other end of the first transmission sleeve. A third gear disk is fixedly connected to the first threaded rod at the location corresponding to the second gear disk. One side of the third gear disk meshes with the second gear disk for transmission.

[0016] Furthermore, the second transmission mechanism includes a short shaft rotatably connected to the inside of the machine body. One end of the short shaft is fixedly connected to a second driven plate. One side of the second driven plate abuts against a second actuating plate. One end of the second transmission sleeve is fixedly connected to a fourth gear disk. The other end of the short shaft is fixedly connected to a fifth gear disk corresponding to the fourth gear disk. One side of the fifth gear disk meshes with the fourth gear disk for transmission.

[0017] Furthermore, the top of the machine body has a mounting hole, and an electron microscope is fixedly connected inside the mounting hole. The camera of the electron microscope is located directly above the placement tray. A temperature sensor and a temperature control module are fixedly connected inside the machine body. A control panel is fixedly connected outside the sealed door. The control panel is electrically connected to the temperature sensor, the electron microscope, and the temperature control module. Ventilation holes are provided on both sides of the machine body corresponding to the temperature control module. The ventilation holes do not communicate with the interior of the machine body.

[0018] A method for preparing peripheral blood autologous immune cells, which utilizes the peripheral blood autologous immune cell culture device described in any one of the above-mentioned methods, comprises the following steps:

[0019] Step 1: The traction plate is pushed by starting the push rod motor. The movement of the traction plate drives the connecting rod to move synchronously. When the movement of the connecting rod causes the first actuating plate to be in close contact with the first driven plate, the second actuating plate separates from the second driven plate.

[0020] Step 2: The forward start of the drive motor drives the first gear disk to rotate forward. The forward rotation of the first gear disk drives the gear column to rotate forward. The forward rotation of the gear column drives the connecting rod to rotate. The rotation of the connecting rod drives the first actuating plate and the second actuating plate to rotate synchronously. The rotation of the first actuating plate drives the first driven plate to rotate synchronously. The rotation of the first driven plate drives the first transmission sleeve to rotate. The rotation of the first transmission sleeve drives the second gear disk to rotate. The rotation of the second gear disk drives the third gear disk to rotate. At the same time, it drives the first threaded rod to rotate forward. The forward rotation of the first threaded rod drives the moving block to move. At the same time, it drives the connecting plate to move smoothly along the guide rod through the guide plate.

[0021] When the moving plate moves synchronously, it drives the second threaded rod and the internal threaded sleeve to move synchronously. The rotation of the internal threaded sleeve drives the placement plate to move synchronously. When the moving block moves to the end of the thread of the first threaded rod, the drive motor stops working. When the moving block moves to the end of the thread of the first threaded rod, the placement plate moves out of the machine body. At this time, the immune cell sample to be cultured is placed inside the placement plate.

[0022] Step 3: The reverse start of the drive motor drives the first gear disk to rotate in the opposite direction. The reverse rotation of the first gear disk drives the first threaded rod to rotate in the opposite direction. The reverse rotation of the first threaded rod drives the moving block to move in the opposite direction, and at the same time, it drives the placement plate into the interior of the machine body. When the moving block moves to the end of the thread of the first threaded rod again, the drive motor stops working again. Then, the sealing door is closed, and the internal temperature of the machine body is set through the control panel. At the same time, the internal temperature of the machine body is monitored and controlled through the temperature sensor and temperature control module.

[0023] Step 4: When it is necessary to examine the cultured immune cells, the push rod motor is activated to retract the traction plate, which in turn moves the connecting rod. The movement of the connecting rod causes the first actuating plate to separate from the first driven plate. At the same time, since the length of the gear column is greater than the thickness of the first gear disk, the gear column maintains meshing transmission as it moves.

[0024] When the linkage moves, it causes the second actuating plate to come into close contact with the second driven plate. The drive motor starts forward again and synchronously drives the first gear disc to rotate forward. The forward rotation of the first gear disc drives the gear column to rotate forward, and at the same time, it drives the second actuating plate to rotate via the linkage. The rotation of the second actuating plate drives the second driven plate to rotate forward. When the second driven plate rotates forward, it drives the short shaft to rotate. The rotation of the short shaft drives the fifth gear disc to rotate. The rotation of the fifth gear disc synchronously drives the fourth gear disc to rotate. The rotation of the fourth gear disc drives the second transmission sleeve to rotate. The rotation of the second transmission sleeve drives... The rotation of the worm gear drives the worm wheel to rotate synchronously, which in turn drives the second threaded rod. The rotation of the second threaded rod causes the internal threaded sleeve to move synchronously upward through the limiting rod, moving the placement disk upward. After the placement disk rises, immune cells are observed and processed using an electron microscope. When the drive motor starts in reverse, it drives the first connecting rod to rotate in the opposite direction, which in turn drives the second transmission sleeve and the worm gear to rotate synchronously in the opposite direction. The reverse rotation of the worm gear drives the reverse rotation of the second threaded rod, which in turn resets the placement disk. After resetting, the drive motor stops working.

[0025] Compared with existing technologies, the beneficial effects of this invention are:

[0026] 1. The present invention performs power output, power conversion and power transmission by installing a drive mechanism, a control mechanism, a first transmission mechanism and a push mechanism. The drive mechanism drives the first gear disk to rotate through a drive motor, which in turn drives the connecting rod to rotate, and simultaneously drives the first threaded rod to rotate. The rotation of the first threaded rod drives the connecting plate to move.

[0027] 2. This invention performs secondary power transmission on the equipment by installing a second transmission mechanism and a height adjustment mechanism. The rotation of the connecting rod drives the rotation of the second transmission sleeve and the worm gear, the rotation of the worm gear drives the rotation of the second threaded rod, and the rotation of the second threaded rod drives the placement plate to move up and down.

[0028] In summary, this device features a novel design and simple operation. It can not only seal and culture immune cell samples in a placement tray, but also effectively solve the problem of contamination when immune cells are removed for inspection by adjusting the height of the placement tray, thus effectively improving the culture and inspection results of immune cells. Attached Figure Description

[0029] Figure 1 This is a first overall top-view three-dimensional structural diagram of the culture device and preparation method for peripheral blood autologous immune cells proposed in this invention;

[0030] Figure 2This is a second overall top-view three-dimensional structural diagram of the culture device and preparation method for peripheral blood autologous immune cells proposed in this invention;

[0031] Figure 3 This is a partial cross-sectional front view of the culture device and preparation method for peripheral blood autologous immune cells proposed in this invention.

[0032] Figure 4 This is a top-view three-dimensional structural diagram of a partial cross-section of the organism, illustrating the culture device and preparation method for peripheral blood autologous immune cells proposed in this invention.

[0033] Figure 5 This is a top-view three-dimensional structural diagram of the driving mechanism, control mechanism, and pushing mechanism of the peripheral blood autologous immune cell culture device and preparation method proposed in this invention.

[0034] Figure 6 This is a top-view three-dimensional structural diagram of the first and second transmission mechanisms of the peripheral blood autologous immune cell culture device and preparation method proposed in this invention.

[0035] Figure 7 This is a top-view three-dimensional structural diagram of the driving mechanism and control mechanism of the peripheral blood autologous immune cell culture device and preparation method proposed in this invention;

[0036] Figure 8 This is a schematic diagram of the height adjustment mechanism of the culture device and preparation method for peripheral blood autologous immune cells proposed in this invention.

[0037] Figure 9 This is a top-view three-dimensional structural diagram of the worm gear structure of the peripheral blood autologous immune cell culture device and preparation method proposed in this invention;

[0038] Figure 10 This is a top-view three-dimensional structural diagram of the first and second transmission mechanisms of the peripheral blood autologous immune cell culture device and preparation method proposed in this invention.

[0039] Explanation of reference numerals in the attached figures:

[0040] 1. Body; 2. Drive motor; 3. First gear disc; 4. First threaded rod; 5. Moving block; 6. Connecting plate; 7. Guide plate; 8. Guide rod; 9. Second threaded rod; 10. Third gear disc; 11. Connecting rod; 12. Gear column; 13. First transmission sleeve; 14. Second gear disc; 15. First driven plate; 16. First actuating plate; 17. Support frame; 18. Worm; 19. Worm wheel; 20. Internal threaded sleeve; 21. Placement plate; 22. Limiting rod; 23. Balance plate; 24. Second transmission sleeve; 25. Fourth gear disc; 26. Short shaft; 27. Fifth gear disc; 28. Second driven plate; 29. ​​Push rod motor; 30. Traction plate; 31. Slide plate; 32. Slide rod; 33. Temperature sensor; 34. Temperature control module; 35. Electron microscope; 36. Sealing door; 37. Control panel; 38. Second actuating plate. Detailed Implementation

[0041] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0042] In the description of this invention, it should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," and "counterclockwise," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0043] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, features defined with "first" and "second" may explicitly or implicitly include one or more of the stated features. In the description of this invention, "a plurality of" means two or more, unless otherwise explicitly specified. Furthermore, the terms "installed," "connected," and "linked" should be interpreted broadly; for example, they may refer to a fixed connection, a detachable connection, or an integral connection; they may refer to a mechanical connection or an electrical connection; they may refer to a direct connection or an indirect connection through an intermediate medium; and they may refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances. The invention will now be described in further detail with reference to the accompanying drawings.

[0044] Example 1

[0045] Reference Figure 1-10 A peripheral blood autologous immune cell culture device includes a body 1 and a sealed door 36 hinged to the outside of the body 1, and also includes a motor frame installed inside the body 1. The motor frame is provided with a drive mechanism, which includes a drive motor 2 fixedly connected to the motor frame. The output shaft of the drive motor 2 is fixedly connected to a first gear disk 3. The start of the drive motor 2 drives the first gear disk 3 to rotate synchronously through the output shaft.

[0046] The control mechanism is installed inside the body 1. One side of the control mechanism is connected to the drive mechanism. The control mechanism includes a push rod motor 29 fixedly connected to the inside of the body 1, a traction plate 30 fixedly connected to the output shaft of the push rod motor 29, and a connecting rod 11 rotatably connected to the traction plate 30 through a bearing. The drive mechanism drives the connecting rod 11 to rotate.

[0047] The pushing mechanism is installed inside the body 1. A first transmission mechanism is set between one side of the pushing mechanism and the control mechanism. The pushing mechanism includes a first threaded rod 4 rotatably connected to the inside of the body 1, a moving block 5 threadedly connected to the first threaded rod 4, and a connecting plate 6 fixedly connected to one side of the moving block 5. The rotation of the connecting rod 11 drives the rotation of the first threaded rod 4 through the first transmission mechanism. The rotation of the first threaded rod 4 drives the moving block 5 to move. The movement of the moving block 5 synchronously drives the connecting plate 6 to move.

[0048] The height adjustment mechanism is installed inside the body 1. A second transmission mechanism is provided between one side of the height adjustment mechanism and the control mechanism. The height adjustment mechanism includes a second threaded rod 9 rotatably connected to the moving block 5, an internal threaded sleeve 20 threadedly connected to the second threaded rod 9, and a placement plate 21 fixedly connected to the top of the internal threaded sleeve 20. The rotation of the linkage rod 11 drives the second threaded rod 9 to rotate through the second transmission mechanism. The rotation of the second threaded rod 9 drives the internal threaded sleeve 20 to move, and simultaneously drives the placement plate 21 to move.

[0049] In this invention, the control mechanism also includes a gear column 12 fixedly connected to one end of the linkage 11. One side of the gear column 12 meshes with the first gear disk 3 for transmission. A first actuating plate 16 and a second actuating plate 38 are symmetrically fixed on the linkage 11.

[0050] In this invention, the pushing mechanism also includes a guide rod 8 fixedly connected to the inside of the body 1. A guide plate 7 is fixedly connected to the connecting plate 6 at the guide rod 8. A guide hole is opened on the guide plate 7. The guide rod 8 moves through the inside of the guide hole. Two slide rods 32 are symmetrically fixedly connected inside the body 1. The same slide plate 31 is movably sleeved on the two slide rods 32. A first through hole is opened on the slide plate 31 at the position corresponding to the internal threaded sleeve 20. The internal threaded sleeve 20 is movably sleeved inside the first through hole.

[0051] In this invention, the height adjustment mechanism also includes a support frame 17 fixedly connected to the bottom of the connecting plate 6. A worm gear 18 is rotatably connected to one side of the support frame 17. A worm wheel 19 is fixedly connected to the second threaded rod 9 at the location corresponding to the worm gear 18. The worm wheel 19 meshes with the worm gear 18 for transmission. A limit slider is fixedly connected to one side of the worm gear 18. A second transmission sleeve 24 is rotatably connected inside the body 1. A moving groove is opened on the second transmission sleeve 24 at the location corresponding to the limit slider. The worm gear 18 is movably sleeved inside the second transmission sleeve 24. The limit slider is movably sleeved inside the moving groove. A balance plate 23 is fixedly connected below the internal threaded sleeve 20. Two limit rods 22 are fixedly connected between the balance plate 23 and the placement plate 21. A second through hole is opened on the slide plate 31 at the location corresponding to the two limit rods 22. The movement of the placement plate 21 is limited by the two limit rods 22. The two limit rods 22 are movably sleeved inside the corresponding second through holes.

[0052] In this invention, the first transmission mechanism includes a first transmission sleeve 13 rotatably connected to the inner bottom end of the body 1. One end of the first transmission sleeve 13 is fixedly connected to a first driven plate 15. One side of the first driven plate 15 abuts against a first actuating plate 16. The other end of the first transmission sleeve 13 is fixedly connected to a second gear disk 14. A third gear disk 10 is fixedly connected to the first threaded rod 4 at the location corresponding to the second gear disk 14. One side of the third gear disk 10 meshes with the second gear disk 14 for transmission. The rotation of the second gear disk 14 synchronously drives the third gear disk 10 to rotate.

[0053] In this invention, the second transmission mechanism includes a short shaft 26 rotatably connected to the inside of the body 1. One end of the short shaft 26 is fixedly connected to a second driven plate 28. One side of the second driven plate 28 abuts against a second actuating plate 38. One end of the second transmission sleeve 24 is fixedly connected to a fourth gear disk 25. The other end of the short shaft 26 is fixedly connected to a fifth gear disk 27 corresponding to the fourth gear disk 25. One side of the fifth gear disk 27 meshes with the fourth gear disk 25 for transmission.

[0054] In this invention, the top of the body 1 has a mounting hole, and an electron microscope 35 is fixedly connected inside the mounting hole. The camera of the electron microscope 35 is located directly above the placement tray 21. The electron microscope 35 is used to view and process the immune cell samples inside the body 1. A temperature sensor 33 and a temperature control module 34 are fixedly connected inside the body 1. A control panel 37 is fixedly connected to the outside of the sealed door 36. The control panel 37 is electrically connected to the temperature sensor 33, the electron microscope 35, and the temperature control module 34 respectively. The temperature sensor 33 and the temperature control module 34 are used to monitor and control the set temperature inside the body 1 in real time. Ventilation holes are opened on both sides of the body 1 corresponding to the temperature control module 34. The ventilation holes are not connected to the inside of the body 1.

[0055] Example 2

[0056] This embodiment provides a technical solution based on Embodiment 1: a method for preparing peripheral blood autologous immune cells, the steps of which are as follows:

[0057] Step 1: The traction plate 30 is pushed by starting the push rod motor 29. The movement of the traction plate 30 synchronously drives the connecting rod 11 to move. When the connecting rod 11 moves, the first actuating plate 16 and the first driven plate 15 are closely attached, and at the same time the second actuating plate 38 is separated from the second driven plate 28.

[0058] Step 2: The forward start of the drive motor 2 drives the first gear disk 3 to rotate forward. The forward rotation of the first gear disk 3 drives the gear column 12 to rotate forward. The forward rotation of the gear column 12 drives the connecting rod 11 to rotate. The rotation of the connecting rod 11 drives the first actuating plate 16 and the second actuating plate 38 to rotate synchronously. The rotation of the first actuating plate 16 drives the first driven plate 15 to rotate synchronously. The rotation of the first driven plate 15 drives the first transmission sleeve 13 to rotate. The rotation of the first transmission sleeve 13 drives the second gear disk 14 to rotate. The rotation of the second gear disk 14 drives the third gear disk 10 to rotate. At the same time, it drives the first threaded rod 4 to rotate forward. The forward rotation of the first threaded rod 4 drives the moving block 5 to move. At the same time, it drives the connecting plate 6 to move smoothly along the guide rod 8 through the guide plate 7.

[0059] When the moving plate 6 moves synchronously, it drives the second threaded rod 9 and the internal threaded sleeve 20 to move synchronously. The rotation of the internal threaded sleeve 20 drives the placement plate 21 to move synchronously. When the moving block 5 moves to the end of the thread of the first threaded rod 4, the drive motor 2 stops working. When the moving block 5 moves to the end of the thread of the first threaded rod 4, the placement plate 21 is moved out of the body 1. At this time, the cultured immune cell sample is placed inside the placement plate 21.

[0060] Step 3: The reverse start of the drive motor 2 drives the first gear disk 3 to rotate in the opposite direction. The reverse rotation of the first gear disk 3 drives the first threaded rod 4 to rotate in the opposite direction. The reverse rotation of the first threaded rod 4 drives the moving block 5 to move in the opposite direction. At the same time, it drives the placement plate 21 into the interior of the machine body 1. When the moving block 5 moves to the end of the thread of the first threaded rod 4 again, the drive motor 2 stops working again. Then, the sealing door 36 is closed. The internal temperature of the machine body 1 is set through the control panel 37. At the same time, the internal temperature of the machine body 1 is monitored and controlled through the temperature sensor 33 and the temperature control module 34.

[0061] Step 4: When it is necessary to examine the cultured immune cells, the push rod motor 29 is activated to retract the traction plate 30, which in turn moves the connecting rod 11. The movement of the connecting rod 11 causes the first actuating plate 16 to separate from the first driven plate 15. Meanwhile, since the length of the gear column 12 is greater than the thickness of the first gear disk 3, the gear column 12 maintains meshing transmission as it moves.

[0062] When the linkage 11 moves, it causes the second actuating plate 38 to closely engage with the second driven plate 28. The drive motor 2 then restarts in the forward direction and synchronously drives the first gear disk 3 to rotate in the forward direction. The forward rotation of the first gear disk 3 drives the gear column 12 to rotate in the forward direction, and simultaneously, through the linkage 11, it drives the second actuating plate 38 to rotate. The rotation of the second actuating plate 38 drives the second driven plate 28 to rotate in the forward direction. When the second driven plate 28 rotates in the forward direction, it drives the short shaft 26 to rotate. The rotation of the short shaft 26 drives the fifth gear disk 27 to rotate. The rotation of the fifth gear disk 27 synchronously drives the fourth gear disk 25 to rotate. The rotation of the fourth gear disk 25 drives the second transmission sleeve 24 to rotate. The rotation of the second transmission sleeve 24... The drive motor 2 drives the worm gear 18 to rotate, which in turn drives the worm wheel 19 to rotate synchronously. The rotation of the worm wheel 19 drives the second threaded rod 9 to rotate, which in turn drives the internal threaded sleeve 20 to move the placement tray 21 upward synchronously via the limiting rod 22. After the placement tray 21 rises, the immune cells are observed and processed through the electron microscope 35. When the drive motor 2 starts in reverse, it drives the first connecting rod 11 to rotate in reverse, which in turn drives the second transmission sleeve 24 and the worm gear 18 to rotate synchronously in reverse. The reverse rotation of the worm gear 18 drives the second threaded rod 9 to rotate in reverse, which in turn drives the placement tray 21 to reset. After resetting, the drive motor 2 stops working.

[0063] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A device for culturing peripheral blood autologous immune cells, comprising a body (1) and a sealed door (36) hinged to the outside of the body (1), characterized in that, Also includes: A motor frame is installed inside the body (1), and a drive mechanism is provided on the motor frame; The control mechanism is installed inside the body (1). One side of the control mechanism is connected to the drive mechanism. The control mechanism includes a push rod motor (29) fixedly connected to the inside of the body (1), a traction plate (30) fixedly connected to the output shaft of the push rod motor (29), and a connecting rod (11) rotatably connected to the traction plate (30) through a bearing. The drive mechanism drives the connecting rod (11) to rotate. The pushing mechanism is installed inside the body (1). A first transmission mechanism is provided between one side of the pushing mechanism and the control mechanism. The pushing mechanism includes a first threaded rod (4) rotatably connected to the inside of the body (1), a moving block (5) threadedly connected to the first threaded rod (4), and a connecting plate (6) fixedly connected to one side of the moving block (5). The rotation of the connecting rod (11) drives the rotation of the first threaded rod (4) through the first transmission mechanism. The rotation of the first threaded rod (4) drives the moving block (5) to move. The movement of the moving block (5) synchronously drives the connecting plate (6) to move. The height adjustment mechanism is installed inside the body (1). A second transmission mechanism is provided between one side of the height adjustment mechanism and the control mechanism. The height adjustment mechanism includes a second threaded rod (9) rotatably connected to the moving block (5), an internal threaded sleeve (20) threadedly connected to the second threaded rod (9), and a placement plate (21) fixedly connected to the top of the internal threaded sleeve (20). The rotation of the linkage rod (11) drives the second threaded rod (9) to rotate through the second transmission mechanism. The rotation of the second threaded rod (9) drives the internal threaded sleeve (20) to move and synchronously drives the placement plate (21) to move.

2. The peripheral blood autologous immune cell culture device according to claim 1, characterized in that, The drive mechanism includes a drive motor (2) fixedly connected to the motor frame, and the output shaft of the drive motor (2) is fixedly connected to a first gear disk (3).

3. The peripheral blood autologous immune cell culture device according to claim 2, characterized in that, The control mechanism also includes a gear column (12) fixedly connected to one end of the linkage (11). One side of the gear column (12) meshes with the first gear disk (3) for transmission. A first actuating plate (16) and a second actuating plate (38) are symmetrically fixed on the linkage (11).

4. The peripheral blood autologous immune cell culture device according to claim 3, characterized in that, The pushing mechanism also includes a guide rod (8) fixedly connected to the inside of the body (1). The connecting plate (6) is fixedly connected to a guide plate (7) at the position corresponding to the guide rod (8). A guide hole is provided on the guide plate (7). The guide rod (8) moves through the inside of the guide hole. Two slide rods (32) are symmetrically fixedly connected inside the body (1). The same slide plate (31) is movably sleeved on the two slide rods (32). A first through hole is provided at the position corresponding to the internal threaded sleeve (20) of the slide plate (31). The internal threaded sleeve (20) is movably sleeved inside the first through hole.

5. The peripheral blood autologous immune cell culture device according to claim 1, characterized in that, The height adjustment mechanism also includes a support frame (17) fixedly connected to the bottom of the connecting plate (6). A worm gear (18) is rotatably connected to one side of the support frame (17). A worm wheel (19) is fixedly connected to the second threaded rod (9) at the position corresponding to the worm gear (18). The worm wheel (19) meshes with the worm gear (18) for transmission. A limit slider is fixedly connected to one side of the worm gear (18). A second transmission sleeve (24) is rotatably connected inside the machine body (1). A limit slider is located on the second transmission sleeve (24). A movable groove is provided at the slider. The worm gear (18) is movably sleeved inside the second transmission sleeve (24). The limiting slider is movably sleeved inside the movable groove. A balance plate (23) is fixedly connected below the internal thread sleeve (20). Two limiting rods (22) are fixedly connected between the balance plate (23) and the placement plate (21). The slide plate (31) is provided with a second through hole corresponding to the two limiting rods (22). The two limiting rods (22) are movably sleeved inside the corresponding second through hole.

6. The peripheral blood autologous immune cell culture device according to claim 3, characterized in that, The first transmission mechanism includes a first transmission sleeve (13) rotatably connected to the inner bottom end of the body (1). One end of the first transmission sleeve (13) is fixedly connected to a first driven plate (15). One side of the first driven plate (15) abuts against a first actuating plate (16). The other end of the first transmission sleeve (13) is fixedly connected to a second gear disk (14). The first threaded rod (4) is fixedly connected to a third gear disk (10) at the position corresponding to the second gear disk (14). One side of the third gear disk (10) meshes with the second gear disk (14) for transmission.

7. The peripheral blood autologous immune cell culture device according to claim 3, characterized in that, The second transmission mechanism includes a short shaft (26) rotatably connected to the inside of the body (1). One end of the short shaft (26) is fixedly connected to a second driven plate (28). One side of the second driven plate (28) abuts against a second actuating plate (38). One end of the second transmission sleeve (24) is fixedly connected to a fourth gear disk (25). The other end of the short shaft (26) is fixedly connected to a fifth gear disk (27) corresponding to the fourth gear disk (25). One side of the fifth gear disk (27) meshes with the fourth gear disk (25) for transmission.

8. The peripheral blood autologous immune cell culture device according to claim 1, characterized in that, The top of the body (1) has an installation hole, and an electron microscope (35) is fixedly connected inside the installation hole. The camera of the electron microscope (35) is located directly above the placement plate (21). A temperature sensor (33) and a temperature control module (34) are fixedly connected inside the body (1). A control panel (37) is fixedly connected outside the sealing door (36). The control panel (37) is electrically connected to the temperature sensor (33), the electron microscope (35), and the temperature control module (34). Ventilation holes are opened on both sides of the body (1) corresponding to the temperature control module (34). The ventilation holes are not connected to the interior of the body (1).

9. A method for preparing peripheral blood autologous immune cells, which is applicable to the peripheral blood autologous immune cell culture device described in any one of claims 1-8, characterized in that, The steps are as follows: Step 1: The traction plate (30) is pushed by starting the push rod motor (29). The traction plate (30) moves synchronously, driving the connecting rod (11) to move. When the connecting rod (11) moves, the first actuating plate (16) and the first driven plate (15) are closely attached, and at the same time the second actuating plate (38) and the second driven plate (28) are separated. Step 2: The forward start of the drive motor (2) drives the first gear disk (3) to rotate in the forward direction. The forward rotation of the first gear disk (3) drives the gear column (12) to rotate in the forward direction. The forward rotation of the gear column (12) drives the connecting rod (11) to rotate. The rotation of the connecting rod (11) drives the first actuating plate (16) and the second actuating plate (38) to rotate synchronously. The rotation of the first actuating plate (16) drives the first driven plate (15) to rotate synchronously. The rotation of the first driven plate (15) drives the first transmission sleeve (13) to rotate. The rotation of the first transmission sleeve (13) drives the second gear disk (14) to rotate. The rotation of the second gear disk (14) drives the third gear disk (10) to rotate. At the same time, it drives the first threaded rod (4) to rotate in the forward direction. The forward rotation of the first threaded rod (4) drives the moving block (5) to move. At the same time, it drives the connecting plate (6) to move smoothly along the guide rod (8) through the guide plate (7). When the movement of the linkage plate (6) synchronously drives the second threaded rod (9) and the inner threaded sleeve (20) to move synchronously, the rotation of the inner threaded sleeve (20) drives the placement plate (21) to move synchronously. When the moving block (5) moves to the end of the thread of the first threaded rod (4), the drive motor (2) stops working. When the moving block (5) moves to the end of the thread of the first threaded rod (4), the placement plate (21) moves out of the interior of the machine body (1). At this time, the immune cell sample to be cultured is placed inside the placement plate (21). Step 3: The reverse start of the drive motor (2) drives the first gear disk (3) to rotate in the opposite direction. The reverse rotation of the first gear disk (3) drives the first threaded rod (4) to rotate in the opposite direction. The reverse rotation of the first threaded rod (4) drives the moving block (5) to move in the opposite direction. At the same time, it drives the placement plate (21) into the interior of the machine body (1). When the moving block (5) moves to the end of the thread of the first threaded rod (4) again, the drive motor (2) stops working again. Then, the sealing door (36) is closed. The internal temperature of the machine body (1) is set through the control panel (37). At the same time, the internal temperature of the machine body (1) is monitored and controlled through the temperature sensor (33) and the temperature control module (34). Step 4: When it is necessary to examine the cultured immune cells, the push rod motor (29) is activated to retract the traction plate (30) and simultaneously drive the connecting rod (11) to move. The movement of the connecting rod (11) causes the first actuating plate (16) to separate from the first driven plate (15). At the same time, since the length of the gear column (12) is greater than the thickness of the first gear disk (3), the gear column (12) always maintains meshing transmission when it moves. When the linkage (11) moves, it causes the second actuating plate (38) to closely engage with the second driven plate (28), and the drive motor (2) starts forward again and synchronously drives the first gear disk (3) to rotate forward. The forward rotation of the first gear disk (3) drives the gear column (12) to rotate forward, and at the same time, it drives the second actuating plate (38) to rotate through the linkage (11). The rotation of the second actuating plate (38) drives the second driven plate (28) to rotate forward. When the forward rotation of the second driven plate (28) drives the short shaft (26) to rotate, the rotation of the short shaft (26) drives the fifth gear disk (27) to rotate. The rotation of the fifth gear disk (27) synchronously drives the fourth gear disk (25) to rotate. The rotation of the fourth gear disk (25) drives the second transmission sleeve (24) to rotate. The rotation of the second transmission sleeve (24) The drive motor (2) drives the worm (18) to rotate, and the rotation of the worm (18) drives the worm wheel (19) to rotate synchronously. The rotation of the worm wheel (19) drives the second threaded rod (9) to rotate, and the rotation of the second threaded rod (9) drives the internal threaded sleeve (20) to move synchronously upward through the limit rod (22) and the placement plate (21). After the placement plate (21) rises, the immune cells are observed and processed by the electron microscope (35). When the drive motor (2) starts in reverse, it drives the first linkage rod (11) to rotate in reverse, and at the same time drives the second transmission sleeve (24) and the worm (18) to rotate synchronously in reverse. The reverse rotation of the worm (18) drives the second threaded rod (9) to rotate in reverse, and the reverse rotation of the second threaded rod (9) drives the placement plate (21) to reset. After resetting, the drive motor (2) stops working.

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