A method for collecting and detecting psychoactive substances in an environment

Abstract

The present application belongs to the technical field of drug detection, and particularly relates to a collection and detection method of psychoactive substances in the environment. The collection method comprises the following steps: (1) using a hydrophilic inorganic material to be wetted in a solvent; (2) using the wetted hydrophilic inorganic material to wipe and sample in the environment; and (3) using a stabilizer to keep the sample stable. In the present application, the detection sensitivity of the target substance is improved after the collected sample is treated with the stabilizer, and even low-concentration substances can be effectively detected.

Description

Technical Field

[0001] This invention belongs to the field of drug detection technology, specifically relating to a method for collecting and detecting psychoactive substances in the environment. Background Technology

[0002] With enhanced detection methods and increased enforcement efforts, large-scale drug manufacturing has become increasingly difficult to detect. Many potential drug-related activities take place in small areas, and drug production can be accomplished using only conventional chemical laboratory equipment and chemicals, making it easy to conceal within normal laboratory activities and difficult to detect. Therefore, the demand for on-site detection in drug use locations and detection of laboratory drug manufacturing environments is growing.

[0003] Currently, drug testing mainly relies on urine, blood, hair, sweat, and saliva tests, as well as emerging wastewater drug testing technology. Each method has its advantages and limitations, and is suitable for different testing scenarios and needs. Among them, urine testing is widely used due to its simplicity and low cost, but it suffers from a short detection window. Blood testing can provide direct evidence, but it requires specialized equipment and technicians, and is relatively expensive. Hair testing can trace long-term drug use history, but the testing process is complex. Sweat and saliva testing are convenient to operate, but their accuracy can be affected by various factors and needs improvement. Wastewater drug testing technology, as an emerging anti-drug technology, can estimate the types of drugs abused, consumption, and the scale of drug users in a specific area by analyzing the chemical components in wastewater or metabolites in human urine, but it cannot quickly locate the user after testing.

[0004] Therefore, it is necessary to develop a rapid on-site sampling and detection method for laboratory drug use and manufacturing environments. Summary of the Invention

[0005] This invention provides a rapid on-site sampling and processing detection method for laboratory drug use and manufacturing environments. This method overcomes the shortcomings of existing detection methods, such as easy contamination, low sensitivity, and false negatives. It is also simple to operate, low in cost, and has high sensitivity and accuracy.

[0006] To achieve the above objectives, the present invention can adopt the following technical solutions:

[0007] The present invention provides a method for collecting psychoactive substances in the environment, comprising: (1) wetting a hydrophilic inorganic material in a solvent; (2) wiping the sample in the environment with the wetted hydrophilic inorganic material; and (3) stabilizing the sample with a stabilizer, wherein the stabilizer is a mixed solvent composed of formic acid, methanol and water in a volume ratio of (6-8):(2-4):(0.2-0.4).

[0008] Preferably, the volume ratio of the formic acid, methanol and water is 7:3:0.3.

[0009] Preferably, the hydrophilic inorganic material is selected from any one of degreased cotton swabs, glass wool, asbestos, or dust-free paper; and / or the solvent is selected from one or more of acetonitrile, methanol, ethanol, or pure water.

[0010] Preferably, the sources for wiping sampling in the above-mentioned environment include door handles, walls, tabletops, or instrument surfaces.

[0011] Preferably, interference from grease is avoided during the sampling process.

[0012] Preferably, the aforementioned psychoactive substance is etomidate.

[0013] In another aspect, the present invention provides a method for detecting psychoactive substances in the environment, comprising: (1) sampling using the sampling method of the present invention; (2) sonicating for 3 min, vortexing for 2 min, and centrifuging at 15000 r / min for 3 min after sampling, and filtering the supernatant through a microporous membrane to obtain filtrate; (3) performing gas chromatography-mass spectrometry detection on the filtrate.

[0014] Preferably, the filter membrane has a size of 13 mm x 0.1 μm.

[0015] Preferably, the conditions for the above gas chromatography-mass spectrometry detection are as follows:

[0016] The ion source conditions for gas chromatography are as follows:

[0017] Carrier gas flow rate (mL / min) 0.5 Airflow velocity (%) 50 Inlet airflow velocity (%) 50 TD temperature (°C) 250 Ion source voltage (V) 4000

[0018] The mass spectrometry conditions are as follows:

[0019] Scanning mode Fullscan Period (0.1ms) 12000 Scan frequency (amu / s) 10000 RF frequency (MHz) 100 Storage Quality Number (AMU) 30 Low quality number (amu) 40 High-quality numbers (amu) 500 Cooldown time (0.1ms) 5000 AC frequency (kHz) 590 Injection time (0.1ms) 310 Multiplier voltage (V) 1100 .

[0020] The beneficial effects of this invention include:

[0021] (1) In this invention, hydrophilic inorganic materials are used for wiping sampling. These materials can effectively adhere to the surface of objects, improve sample collection efficiency, and thus increase the sensitivity of detection. They can quickly absorb target substances, greatly shorten the sampling time, make on-site detection faster, and at the same time reduce interference from non-target substances, thereby improving the selectivity and accuracy of detection.

[0022] (2) In this invention, a stabilizer composed of formic acid, methanol and water in a volume ratio of (6-8):(2-4):(0.2-0.4) is used to treat the collected samples, which improves the detection sensitivity of the target substance and enables even low concentrations of the substance to be effectively detected.

[0023] (3) The gas chromatography-mass spectrometry detection in this invention can use a portable on-site screening mass spectrometer as a detection device, which can quickly and accurately detect drug use sites; the core advantage of the portable on-site screening mass spectrometer is that it is small in size, light in weight, easy to carry, and can quickly respond and reach the detection site.

[0024] (4) The method in this invention reduces manpower and material costs and has high economic benefits. This method is applicable to various types of laboratory testing, including but not limited to environmental monitoring, food safety testing, medical testing and other fields.

[0025] (5) The method of the present invention can significantly improve the detection speed and reduce the environmental impact while ensuring the detection quality, and at the same time ensure the safety and economy of the operation. Attached Figure Description

[0026] Figure 1 The gas chromatography-mass spectra before the use of stabilizers;

[0027] Figure 2 This is a gas chromatography-mass spectrometry (GC-MS) image after treatment with a stabilizer. Detailed Implementation

[0028] The illustrated embodiments are provided to better illustrate the present invention, but are not intended to limit the scope of the invention to the illustrated embodiments. Therefore, non-essential improvements and adjustments made to the embodiments by those skilled in the art based on the above description of the invention still fall within the protection scope of the present invention.

[0029] The terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit this disclosure. Singular expressions include plural expressions unless they have a distinct meaning in the context. As used herein, it should be understood that terms such as “comprising,” “having,” “including,” are intended to indicate the presence of features, numbers, operations, components, parts, elements, materials, or combinations thereof. The terminology of the invention is disclosed in the specification and is not intended to exclude the possibility that one or more other features, numbers, operations, components, parts, elements, materials, or combinations thereof may be present or added. As used herein, “ / ” may be interpreted as “and” or “or,” depending on the context.

[0030] To better understand the present invention, specific examples are provided below to further illustrate the content of the present invention, but the content of the present invention is not limited to the examples below.

[0031] Example 1

[0032] (1) Prepare an ethanol solution of etomidate standard with a concentration of 1 mg / L;

[0033] (2) Moisten the degreased cotton swabs with acetonitrile and take samples from the etomidate standard solution;

[0034] (3) Place the collected sample into a centrifuge tube and add a stabilizing reagent (composed of formic acid, methanol and water in a volume ratio of 7:3:0.3); then sonicate for 3 min, vortex for 2 min, and centrifuge at 15000 r / min for 3 min. Take the supernatant and inject it into a syringe, then filter it through an organic microporous membrane (13 mm x 0.1 μm) into a sample bottle.

[0035] (4) The processed samples were used in a rapid detection instrument to determine whether there were chemical components related to drug use and drug production in the samples by gas chromatography-mass spectrometry analysis; the conditions for gas chromatography-mass spectrometry detection are shown in Table 1 and Table 2 below.

[0036] Table 1 Ion source conditions

[0037] Carrier gas flow rate (mL / min) 0.5 Airflow velocity (%) 50 Inlet airflow velocity (%) 50 TD temperature (°C) 250 Ion source voltage (V) 4000

[0038] Table 2 Mass Spectrometry Conditions

[0039] Scanning mode Fullscan Period (0.1ms) 12000 Scan frequency (amu / s) 10000 RF frequency (MHz) 100 Storage Quality Number (AMU) 30 Low quality number (amu) 40 High-quality numbers (amu) 500 Cooldown time (0.1ms) 5000 AC frequency (kHz) 590 Injection time (0.1ms) 310 Multiplier voltage (V) 1100

[0040] In addition, samples obtained before stabilization were analyzed and detected using the gas chromatography-mass spectrometry method described above as a comparison.

[0041] The mass spectra before and after stabilizer treatment are as follows: Figure 1 and 2 As shown.

[0042] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all such modifications or substitutions should be covered within the scope of the claims of the present invention.

Claims

1. A method for collecting psychoactive substances from the environment, characterized in that, include: (1) Wet the solvent with a hydrophilic inorganic material; (2) Use moistened hydrophilic inorganic materials to wipe and sample the environment; (3) The sample is kept stable using a stabilizer; the stabilizer is a mixed solvent composed of formic acid, methanol and water in a volume ratio of (6-8):(2-4):(0.2-0.4).

2. The data acquisition method according to claim 1, characterized in that, The volume ratio of formic acid, methanol, and water is 7:3:0.

3.

3. The data acquisition method according to claim 1 or 2, characterized in that, The hydrophilic inorganic material is selected from any one of degreased cotton swabs, glass wool, asbestos, or dust-free paper; and / or the solvent is selected from one or more of acetonitrile, methanol, ethanol, or pure water.

4. The data acquisition method according to claim 1 or 2, characterized in that, Sources for swab sampling in the environment include door handles, walls, tabletops, or instrument surfaces.

5. The data acquisition method according to claim 1 or 2, characterized in that, Avoid interference from grease during the sampling process.

6. The data acquisition method according to claim 1 or 2, characterized in that, The psychoactive substance is etomidate.

7. A method for detecting psychoactive substances in the environment, characterized in that, include: (1) Sampling is performed using the acquisition method described in any one of claims 1 to 7; (2) After sampling, sonicate for 3 min, vortex for 2 min, and then centrifuge at 15000 r / min for 3 min. Take the supernatant and filter it through a microporous membrane to obtain the filtrate; (3) Perform gas chromatography-mass spectrometry detection on the filtrate.

8. The detection method according to claim 7, characterized in that, The filter membrane size is 13mm x 0.1um.

9. The detection method according to claim 7 or 8, characterized in that, The conditions for gas chromatography-mass spectrometry detection are as follows: The ion source conditions for gas chromatography are as follows: The mass spectrometry conditions are as follows: 。

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