Disposable capillary cartridge for high throughput microfluorometric assays

Abstract

The present application relates to nucleic acid quantitative detection technical field, disclose a disposable capillary cassette for high-throughput microfluorometric determination, comprising: a cassette body; a plurality of sample wells arranged in an inclined array on the cassette body, the plurality of sample wells correspond one-to-one to a plurality of liquid injection ends of a standard multi-channel pipettor; a plurality of capillaries arranged one-to-one corresponding to the plurality of sample wells, the capillaries are arranged in the cassette body, one end of the capillaries communicates with the sample wells; a detection window is arranged on the cassette body and located between the sample wells and the other end of the capillaries, an external fluorescence device can excite the sample in the capillary through the detection window, and extract the sample fluorescence intensity and emission spectrum in each capillary. The present application has the advantages of high throughput, portability, complete spectral information, and small sample consumption compared with the prior art.

Description

Technical Field

[0001] This invention relates to the field of nucleic acid quantitative detection technology, and in particular to a disposable capillary cartridge for high-throughput micro-fluorescence assay. Background Technology

[0002] Accurate and highly specific DNA / RNA quantification is crucial for NGS library preparation, qPCR, single-cell genomics, and point-of-care testing. Current technologies typically utilize equipment such as microplate readers, medium-throughput fluorometers, and capillary electrophoresis sequencers for DNA / RNA quantification, which suffer from drawbacks including high sample consumption (20–200 µL), lack of spectral information, low throughput, bulky size, and high cost. For example, multiplate readers consume 20–200 µL of sample per well, providing only total fluorescence intensity or limited filter wavelength information, with a throughput of 96–1536, but only sequential readings. Medium-throughput fluorometers consume 10–200 µL of sample, with a throughput of 8–96, and lack spectral information for each sample. Furthermore, no disposable DNA / RNA quantification device has been disclosed in the current technology.

[0003] Therefore, how to provide a high-throughput, portable measuring device with complete spectral information and low sample consumption is a problem that urgently needs to be solved by those skilled in the art. Summary of the Invention

[0004] The purpose of this invention is to provide a high-throughput, portable measuring device with complete spectral information and low sample consumption, so as to solve the problems existing in the prior art.

[0005] To achieve the above objectives, the present invention provides a disposable capillary cartridge for high-throughput micro-fluorescence assays, comprising: Box body; Multiple sample dispensing ports are arranged in an inclined array on the box body, and each of the multiple sample dispensing ports corresponds to one of the multiple dispensing ends of a standard multichannel pipette. Multiple capillaries are arranged one-to-one with multiple sample dispensing holes. The capillaries are disposed inside the box, with one end connected to the sample dispensing hole. A detection window is set on the housing and located between the sample application port and the other end of the capillary. An external fluorescence device can excite the sample in the capillary through the detection window and extract the fluorescence intensity and emission spectrum of the sample in each capillary.

[0006] Furthermore, the number of capillaries is 2-384.

[0007] Furthermore, the capillary draws the sample from the sample application hole into the capillary through capillary action, and the sample volume required to fill each capillary is 1-5 µL.

[0008] Furthermore, multiple sample wells form multiple rows of inclined and parallel sample well groups, with a minimum interval of 4.5 mm between adjacent sample well groups.

[0009] Furthermore, the length of the capillary corresponds to the position of the sample dispensing orifice.

[0010] Furthermore, the capillary tube is inserted from the side of the box body. After one end of the capillary tube is connected to the sample dispensing hole, the capillary tube is fixed in the box body by UV glue.

[0011] The present invention discloses the following technical effects: 1. High throughput: The maximum number of channels in this invention can reach 384, which is higher than that of the prior art, and there is no need for sequential reading.

[0012] 2. Complete spectral information: This invention opens detection windows for all capillaries, and external fluorescence devices can excite the sample in the capillary through the detection windows and extract the fluorescence intensity and emission spectrum of the sample in each capillary.

[0013] 3. Low sample consumption: The sample volume required to fill all capillaries is 1-5µL, which significantly reduces sample consumption compared with existing technologies.

[0014] 4. Portability: This invention has a simple structure, is easy to carry, and can be used as a disposable detection device for DNA / RNA quantitative detection, with good application prospects and a wide range of applications. Attached Figure Description

[0015] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0016] Figure 1 This is a schematic diagram of the structure of the present invention; Figure 2 This is a schematic diagram of the rear structure of the present invention; The components are: 1. Box body; 2. Capillary tube; 3. Sample dispensing port; 4. Detection window. Detailed Implementation

[0017] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0018] Those skilled in the art will understand that the term "comprising" as used in this application means the presence of the stated features, integers, steps, operations, elements, and / or components, but does not exclude the presence or addition of one or more other features, integers, steps, operations, elements, components, and / or groups thereof. It should be understood that when we say an element is "connected" or "coupled" to another element, it can be directly connected or coupled to the other element, or there may be intermediate elements present. Furthermore, "connected" or "coupled" as used herein can include wireless connections or wireless coupling. The term "and / or" as used herein includes all or any unit and all combinations of one or more associated listed items.

[0019] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0020] Example 1 like Figure 1 and Figure 2 As shown, this embodiment of the invention provides a disposable capillary cartridge for high-throughput micro-fluorescence assays, comprising: Box 1; Multiple sample dispensing holes 3 are arranged in an inclined array on the box 1, and each of the multiple sample dispensing holes 3 corresponds to one of the multiple dispensing ends of a standard multichannel pipette. Multiple capillary tubes 2 are arranged one-to-one with multiple sample dispensing holes 3. The capillary tubes 2 are set inside the box body 1, and one end of them is connected to the sample dispensing hole 3. The detection window 4 is set on the box body 1 and located between the sample application port 3 and the other end of the capillary tube 2. The external fluorescence device can excite the sample in the capillary tube 2 through the detection window 4 and extract the fluorescence intensity and emission spectrum of the sample in each capillary tube 2.

[0021] In this embodiment, the number of capillary tubes 2 is 2-384, specifically 2, 4, 8, 12, 16, 24, 48, 96, 192 or 384 capillary tubes 2.

[0022] In this embodiment, the capillary 2 draws the sample from the sample application port 3 into the capillary 2 through capillary action. The sample volume required to fill each capillary 2 is 1-5µL, without the need for pumping or applying voltage.

[0023] In this embodiment, multiple sample loading holes 3 form multiple rows of inclined and parallel sample loading hole groups 3, with a minimum interval of 4.5 mm between adjacent sample loading hole groups 3.

[0024] In this embodiment, the length of the capillary 2 corresponds to the position of the sample feeding hole 3.

[0025] In this embodiment, the capillary tube 2 is inserted from the side of the box body 1. After one end of the capillary tube 2 is connected to the sample application hole 3, the capillary tube 2 is fixed in the box body 1 by UV glue.

[0026] The performance of this embodiment was compared with that of existing microplate readers, medium-throughput fluorometers, and capillary electrophoresis sequencers. The results are as follows: As can be seen from the above, this embodiment has the advantages of high throughput, portability, complete spectral information, and low sample consumption compared with the prior art.

[0027] Example 2 The difference between this embodiment and Embodiment 1 is that it also includes a portable fluorescence device. The fluorescence device uses a line laser illumination source to simultaneously excite all capillaries 2. The fluorescence device is equipped with a two-dimensional image sensor and a transmission diffraction grating, which can locate the position of each capillary 2 and record the dispersive emission spectrum of each capillary 2.

[0028] In this embodiment, the line density of the transmission diffraction grating is 100 to 1800 lines / mm, providing a spectral resolution of ≤10 nm in the wavelength range of 480–800 nm.

[0029] In this embodiment, the fluorescence device weighs approximately 1-3 kg, facilitating portable operation of the entire device.

[0030] Exciting samples using fluorescence equipment and subsequently extracting the fluorescence intensity and emission spectrum of the samples within each capillary tube 2 are existing technologies and will not be elaborated here.

[0031] In the description of this invention, it should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," and "circumferential" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this invention and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0032] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this invention, "a plurality of" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0033] In this invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0034] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. A disposable capillary cartridge for high-throughput micro-fluorescence assays, characterized in that, include: Box body (1); Multiple sample dispensing holes (3) are arranged in an inclined array on the box body (1), and the multiple sample dispensing holes (3) correspond one-to-one with the multiple dispensing ends of a standard multichannel pipette; Multiple capillaries (2) are arranged one-to-one with multiple sample dispensing holes (3). The capillaries (2) are located inside the box body (1), and one end of each capillary is connected to the sample dispensing hole (3). The detection window (4) is set on the box body (1) and located between the sample application hole (3) and the other end of the capillary (2). The external fluorescence device can excite the sample in the capillary (2) through the detection window (4) and extract the fluorescence intensity and emission spectrum of the sample in each capillary (2).

2. The disposable capillary cartridge for high-throughput micro-fluorescence assay according to claim 1, characterized in that, The number of capillaries (2) is 2-384.

3. The disposable capillary cartridge for high-throughput micro-fluorescence assay according to claim 1, characterized in that, The capillary (2) draws the sample from the sample application hole (3) into the capillary (2) through capillary action, and the sample volume required to fill each capillary (2) is 1-5µL.

4. The disposable capillary cartridge for high-throughput micro-fluorescence assay according to claim 1, characterized in that, Multiple sample wells (3) form multiple rows of inclined and parallel sample well (3) groups, with a minimum interval of 4.5 mm between adjacent sample well (3) groups.

5. The disposable capillary cartridge for high-throughput micro-fluorescence assay according to claim 1, characterized in that, The length of the capillary (2) corresponds to the position of the sample feeding hole (3).

6. The disposable capillary cartridge for high-throughput micro-fluorescence assay according to claim 5, characterized in that, The capillary tube (2) is inserted from the side of the box body (1). When one end of the capillary tube (2) is connected to the sample application hole (3), the capillary tube (2) is fixed in the box body (1) by UV glue.

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