Intelligent liquid chromatography detection, biosynthesis workstation and operating method

Abstract

The application discloses a kind of intelligent liquid chromatography detection, biosynthesis workstation, including work platform and experimental operation robot;Biosynthesis mechanism and HPLC;Biosynthesis mechanism includes heating reaction kettle assembly, heating reaction kettle assembly includes small reaction kettle, and kettle cover is screw-connected on reaction kettle;It further includes the mobile screw cover component that kettle cover is unscrewed;It further includes sampler mechanism, after mobile screw cover component unscrews kettle cover, robot is sampled from small reaction kettle by sampler mechanism;It further includes mixing mechanism, during sample preparation process, mixing mechanism realizes double rotation mixed liquid;Sample bottle assembly and syringe assembly and test tube assembly are installed on the work platform with experimental operation cooperation.The application simultaneously discloses the operation method of the above workstation.The application realizes that biosynthesis reaction, HPLC quantitative, qualitative analysis function is integrated on a platform, realizes biosynthesis and detection linkage integration.

Description

Technical Field

[0001] This invention belongs to the field of chromatography detection and biosynthesis technology, and particularly relates to an intelligent liquid chromatography detection and biosynthesis workstation and its operating method. Background Technology

[0002] HPLC, or High Performance Liquid Chromatography, plays a vital role in industry, particularly in the quantitative and qualitative analysis of substances. HPLC allows for the tracking of reaction progress within a material system, such as during biosynthesis.

[0003] Therefore, given the crucial role of HPLC in the quantitative and qualitative analysis of substances, numerous public reports have documented improvements to HPLC technology. For example, Chinese patent application number CN202520578379.8 discloses a drug delivery device for a high-performance liquid chromatograph. This improved HPLC specifically includes a frame with a sliding housing on the inner wall of its bottom. Multiple test tubes are inserted into the housing. Multiple drug containers are fixedly connected to the inner wall of the top of the frame. Each of the drug containers has an infusion pump at its bottom, and each infusion pump has a delivery tube fixedly connected to its bottom. A connecting tube is fixedly connected to the end of each delivery tube opposite to the infusion pump. This allows the drug to be delivered from different drug containers into the test tubes after the infusion pumps and valves are opened, via the infusion pumps, delivery tubes, connecting tubes, and injection tubes. This allows for drug delivery processing, facilitating adjustments as needed and ensuring flexibility in drug delivery.

[0004] However, existing HPLC systems cannot be functionally integrated with reaction apparatuses such as biosynthetic devices. This is reflected in the fact that during experiments, such as the reaction stage, periodic sampling and testing are required, resulting in a significant workload from sampling, sample processing, and injection to HPLC. Furthermore, there is a severe time lag between sampling and obtaining the detected structure. Therefore, in practical work, if real-time linkage between biosynthetic sampling and HPLC could be achieved, relying entirely on automated operation, it would fundamentally reduce the workload.

[0005] Even more impressively, due to the real-time linkage, the process from sampling to obtaining the detected structure can be completed in a short time. In actual operation, the biosynthesis conditions can be adjusted in real time based on the HPLC detected structure, such as adjusting the reaction temperature and the material feeding ratio, to improve the efficiency of biosynthesis experiments. Summary of the Invention

[0006] Based on the above background, the purpose of this invention is to provide an intelligent liquid chromatography detection and biosynthesis workstation.

[0007] To achieve the above objectives, the present invention adopts the following technical solution:

[0008] A smart liquid chromatography detection and biosynthesis workstation includes a work platform and a robot installed on the work platform for experimental operations;

[0009] It also includes several biosynthesis mechanisms installed on one side of the top of the work platform, as well as an HPLC;

[0010] The biosynthesis apparatus includes a heated reaction vessel assembly, which includes a small reaction vessel and a vessel lid threaded onto the reaction vessel; it also includes a movable lid-screwing assembly for unscrewing the vessel lid.

[0011] It also includes a sampler mechanism for taking samples from a small reactor. After the lid is unscrewed by the moving cap assembly, the robot takes samples from the small reactor through the sampler mechanism.

[0012] A mixing and shaking mechanism is installed on the other side of the top of the working platform. The mixing and shaking mechanism includes a rocker base, and test tube support structures are rotatably connected to the top two sides of the rocker base.

[0013] The bottom of the test tube support structure is fixedly connected to a rotating shaft that is rotatably connected to a rocker base, and a driven pulley is installed on the rotating shaft;

[0014] It also includes a motor mount fixedly installed on the top of the work platform. A motor is mounted on the motor mount, and a drive pulley is fixedly connected to the output shaft of the motor. The drive pulley is connected to the driven pulleys on both sides for transmission.

[0015] The motor's output shaft is fixedly mounted on the rocker stand; during sample preparation, the test tube support structure rotates on its own axis and revolves around the rocker stand to perform dual rotational mixing.

[0016] The work platform is equipped with sample bottle components, syringe components, and test tube components to facilitate experimental operations.

[0017] Preferably, the sampler mechanism includes a pipette assembly and a pipette assembly that cooperates with the pipette assembly;

[0018] The pipette assembly includes a holder on which several pipettes are placed. The pipette assembly includes a pipette rack on which several long stainless steel pipettes are placed. The injection end of the long stainless steel pipettes is integrally formed with a connector for engaging the position of the pipettes.

[0019] Preferably, the sample bottle assembly includes a sample bottle tray on which a plurality of sample bottles are placed;

[0020] The syringe assembly includes a syringe holder and a syringe placed on the syringe holder, with a filter seat installed at the liquid outlet end of the syringe.

[0021] The test tube assembly includes a test tube holder on which test tubes are placed.

[0022] Preferably, the movable cap screwing assembly includes a gripper cylinder, and a cap clamping arm is fixedly installed on each of the two grippers of the gripper cylinder;

[0023] It also includes a U-shaped carriage; the two sides of the U-shaped carriage are sliding ends, and a U-shaped translation frame is slidably connected between the sliding ends;

[0024] A rotary motor bracket is fixedly connected between the sliding ends. A rotary motor is installed on the top of the rotary motor bracket, and the output end of the rotary motor is installed on the top of the cylinder of the gripper cylinder.

[0025] A lifting cylinder is fixedly connected to the U-shaped translation frame to push the gripper cylinder to move up and down. The pushing end of the lifting cylinder is installed at the top of the rotary motor.

[0026] Preferably, the movable cap screwing assembly further includes a lead screw track structure, which includes a lead screw track and a U-shaped translation frame that slides at the bottom of the lead screw track.

[0027] The lead screw is rotatably connected within the lead screw track to push the U-shaped translation frame to move horizontally, and a lead screw motor is installed at the end of the lead screw.

[0028] The bottom sides of the lead screw track are fixedly installed on the top of the work platform by gantry brackets.

[0029] Preferably, the test tube support structure includes an upper perforated plate and a lower support plate spaced apart below the upper perforated plate;

[0030] The rotating shaft is fixedly installed at the bottom center of the lower support plate;

[0031] Several test tubes are inserted between the upper perforated plate and the lower support plate.

[0032] Preferably, the drive pulley has a pair of drive pulley grooves that are spaced apart vertically;

[0033] The driven pulleys on both sides are respectively provided with driven pulley grooves that cooperate with the driving pulley grooves; the driving pulley grooves and the corresponding driven pulley grooves are connected by belt drive.

[0034] Preferably, a reagent bottle holder is fixedly connected to the working platform; reagent bottles are placed on the reagent bottle holder.

[0035] Preferably, the heating reactor assembly further includes a heater that works in conjunction with the small reactor;

[0036] The small reaction vessel can be detachably and fixedly mounted on the heater.

[0037] This invention also discloses the operation method of the above-mentioned intelligent liquid chromatography detection and biosynthesis workstation, including the following steps:

[0038] (1) According to the experimental requirements, put the materials into the small reaction vessel and heat them;

[0039] (2) During the heating process, the movable capping assembly is moved to the location of the small reaction vessel and the vessel cap is unscrewed;

[0040] (3) The robot grabs a pipette from the sampler mechanism, installs a long stainless steel pipette at the inlet of the pipette, moves the robot to the small reaction vessel to take a sample, and moves the capping assembly to screw the cap back on.

[0041] (4) The robot puts the extracted sample into a test tube, and repeats this process to complete the sampling of all small reaction vessels;

[0042] After the robot unscrews the reagent bottle cap, it grabs a pipette, draws the reagent from the bottle, and puts it into a test tube containing the sample.

[0043] (5) The robot picks up the test tube containing the sample and places it on the test tube support structure of the mixing mechanism; driven by the motor, the test tube support structure rotates on its own axis and revolves with the rocker to perform double rotation mixing.

[0044] (6) After the mixture is dissolved, the robot grabs the syringe from the syringe assembly to draw up the sample solution and injects it into the sample bottle. The sample bottle is then loaded onto the HPLC for detection.

[0045] The present invention has the following beneficial effects:

[0046] 1. This invention achieves real-time linkage between HPLC detection and biosynthesis by installing several biosynthesis mechanisms on a working platform. Specifically, there are three biosynthesis mechanisms used to complete biosynthesis experiments. Simultaneously, an HPLC system located in front of the biosynthesis mechanisms is used to monitor and provide feedback on the reaction progress in real time via detection. This integrates the biosynthesis reaction and the quantitative and qualitative analysis functions of HPLC onto a single platform, achieving integrated linkage between biosynthesis and detection. This not only fundamentally reduces a significant amount of experimental labor but also significantly improves experimental efficiency.

[0047] Even more impressively, the linkage mechanism enables the adjustment of biosynthetic process parameters based on real-time HPLC structure detection, fundamentally realizing intelligent experimentation, detection, adjustment, and control.

[0048] 2. This invention discloses a novel structure for mixing and shaking to process sample liquid, which eliminates the drawbacks of manual or mechanical stirring methods for dissolving samples, such as cumbersome operation, large material loss (e.g., mechanical stirring), and poor dissolution effect.

[0049] During the operation, after the test tube containing the sample is placed into the test tube support structure, the robot uses a pipette to draw a fixed volume from the reagent bottle and place it into the test tube.

[0050] Subsequently, driven by the motor, the active pulley rotates. Due to the transmission between the active pulley and the driven pulleys on both sides, the test tube support structure carries the test tubes containing the samples and rotates under the drive of the driven pulleys. This process is called the rotation process.

[0051] Since the output shaft of the motor is also fixedly mounted on the rocker arm, the rocker arm rotates synchronously, and the synchronous rotation of the rocker arm is a revolution process.

[0052] During the aforementioned rotation and revolution, the sample solution in the test tube support structure is thoroughly mixed.

[0053] 3. The movable capping assembly solves the technical bottleneck of the reactor lid being difficult to open during the fully automated sampling process. The movable capping assembly enables automatic capping, which is combined with robot sampling.

[0054] 4. The stainless steel long pipette and pipette enable sampling of high-temperature reaction vessels, solving the problem of difficult automated sampling. Attached Figure Description

[0055] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without creative effort.

[0056] Figure 1 This is a schematic diagram of the overall structure in an embodiment of the present invention;

[0057] Figure 2 This is a schematic diagram of the three-dimensional structure during the detection and synthesis process in an embodiment of the present invention;

[0058] Figure 3 This is one of the structural schematic diagrams of the movable screw cap assembly in an embodiment of the present invention;

[0059] Figure 4 This is a second schematic diagram of the structure of the movable screw cap assembly in an embodiment of the present invention;

[0060] Figure 5 This is one of the structural schematic diagrams of the mixing mechanism in an embodiment of the present invention;

[0061] Figure 6 This is a second schematic diagram of the mixing mechanism in an embodiment of the present invention;

[0062] Figure 7 This is a schematic diagram of the structure of the test tube rack assembly, syringe assembly, pipette assembly, and stainless steel long pipette in an embodiment of the present invention;

[0063] Figure 8 This is a schematic diagram of the sample bottle assembly in an embodiment of the present invention;

[0064] Figure 9 This is an embodiment of the present invention. Figure 2 Top view in the middle;

[0065] Figure 10 This is an embodiment of the present invention. Figure 2 The right view in the image.

[0066] The realization of the objective, functional features and advantages of the present invention will be further explained in conjunction with the embodiments and with reference to the accompanying drawings. Detailed Implementation

[0067] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0068] It should be noted that all directional indications (such as up, down, left, right, front, back, etc.) in the embodiments of the present invention are only used to explain the relative positional relationship and movement of each component in a certain specific posture (as shown in the figure). If the specific posture changes, the directional indication will also change accordingly.

[0069] Furthermore, in this invention, descriptions involving "first," "second," etc., are for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined with "first" or "second" may explicitly or implicitly include at least one of that feature. Additionally, the technical solutions of the various embodiments can be combined with each other, but only on the basis of being achievable by those skilled in the art. When the combination of technical solutions is contradictory or impossible to implement, such a combination of technical solutions should be considered non-existent and not within the scope of protection claimed by this invention.

[0070] Example 1

[0071] like Figure 1-10 As shown, an intelligent liquid chromatography detection and biosynthesis workstation includes a work platform 1 and a robot 2 installed on the work platform 1 for experimental operations.

[0072] Among them, robot 2 is a dual-arm robot 2 disclosed in the prior art. It operates in the same way as the existing robot 2. The two mechanical arms of the dual-arm robot 2 are equipped with grippers, such as conventionally used dual-gripper cylinders as grippers.

[0073] Similar to the existing structure of robot 2 moving on the platform, the top of the work platform 1 is equipped with a track structure 11 that cooperates with robot 2. Specifically, similar to the existing structure of robot 2 moving, robot 2 rotates through a turntable at the bottom, and a moving seat is fixedly installed at the bottom of the turntable. The moving seat is slidably connected to the track structure and moves through a drive screw and a drive motor structure installed on the track structure 11.

[0074] By moving robot 2 on work platform 1, the robot 2 can move to the corresponding workstation to perform operations during the experiment.

[0075] To achieve real-time linkage between HPLC detection and biosynthesis, several biosynthesis mechanisms 3 are installed on the top left rear end of the upper working platform 1. Specifically, there are three biosynthesis mechanisms 3, which are used to complete biosynthesis experiments.

[0076] Meanwhile, an HPLC101 is located in front of the biosynthesis unit 3. It is used to monitor and provide feedback on the reaction process in real time through detection.

[0077] Specifically, the biosynthesis mechanism 3 includes a heated reaction vessel assembly, which includes a small reaction vessel 32 and a vessel cover 321 threadedly connected to the reaction vessel.

[0078] Specifically, the small reaction vessel 32 is a small stainless steel pressure vessel with a volume of 2-5L disclosed in the prior art, and the vessel cover 321 is threadedly connected to the neck of the vessel.

[0079] Similar to the existing heating method for small reactors 32, a heater 31 is installed at the bottom of the small reactor 32. The heater 31 is a conventional heater with magnetic stirring function disclosed in the prior art. The small reactor 32 is fixed to the heating tank of the heater 31 in a conventional manner, such as the reactor body being threaded onto the wall of the heating tank of the heater 31.

[0080] Meanwhile, in order to track the reaction process in real time, the above structure also includes a movable screw cap assembly 4 for unscrewing the lid 321.

[0081] During the sampling process, the moving capping assembly 4 is moved to the location of the corresponding small reactor 32 and the reactor cap 321 is unscrewed to facilitate sampling.

[0082] Therefore, the above structure also includes a sampler mechanism for taking samples from the small reactor 32. After the moving cap assembly 4 unscrews the reactor cap 321, the robot 2 takes samples from the small reactor 32 through the sampler mechanism.

[0083] In order to fully shake, mix and dissolve the sample after sampling, this invention improves a new mixing and shaking mechanism to mix and dissolve the sampled material, simulating manual mixing and dissolving or mechanical stirring.

[0084] Specifically, a mixing and shaking mechanism 5 is installed at the top right rear end of the working platform 1 (corresponding to the working position of the small reaction vessel 32).

[0085] After the sample is fully processed by the mixing mechanism 5, it enters the next stage of sample preparation, which is ready to be loaded onto the chromatograph.

[0086] Therefore, the work platform is equipped with sample bottle components, syringe components, and test tube components to facilitate experimental operations.

[0087] Specifically, the sample vial assembly is located on the top front side of the working platform, in front of HPLC101, the syringe assembly is located on the top right side of the working platform, and the test tube assembly is located on the top right side of the working platform.

[0088] During the experiment, Robot 2 grabs the corresponding sample bottles, syringes, or test tubes as needed.

[0089] Example 2

[0090] like Figure 1-10 As shown, based on the structure of Example 1, in order to solve the problem of fully dissolving and clearing the sample after it is taken from the small reaction vessel 32, so as to facilitate its injection into HPLC 101, the mixing and shaking mechanism 5 disclosed in this invention includes the following structure:

[0091] The mixing and shaking mechanism 5 includes a rocker base 52, and test tube support structures 51 are rotatably connected to the top two sides of the rocker base 52. The test tube support structure 51 is used to place test tubes 8 (after sampling from the small reaction vessel 32, the sample material is placed in the test tube and then placed on the test tube support).

[0092] Meanwhile, the bottom of the test tube support structure 51 is fixedly connected to a rotating shaft that is rotatably connected to the rocker seat 52 (both ends of the rocker seat 52 are respectively equipped with mating bearings), and a driven pulley 53 is installed on the rotating shaft.

[0093] Correspondingly, it also includes a motor base 55 fixedly installed at the top of the work platform 1. A motor is installed on the motor base 55, and a drive pulley 54 is fixedly connected to the output shaft of the motor. The drive pulley 54 is connected to the driven pulleys 53 on both sides for transmission.

[0094] Meanwhile, the motor's output shaft is fixedly installed at the center of the rocker arm 52; during the sample preparation process, the test tube support structure 51 rotates on its own axis and revolves around the rocker arm 52 to perform double rotational mixing.

[0095] Specifically, the driving pulley 54 has a pair of driving pulley grooves spaced apart vertically; the driven pulleys 53 on both sides have driven pulley grooves that cooperate with the driving pulley grooves; the driving pulley grooves and the corresponding driven pulley grooves are connected by belt drive.

[0096] That is, two belts are installed on the drive pulley groove, namely the first belt 541 and the second belt 542. The first belt 541 drives the driven pulley 53 at the rear position, and the second belt 542 drives the driven pulley 53 at the front position.

[0097] During operation, after the test tube containing the sample is placed on the test tube support structure 51, the robot 2 uses a pipette to draw a quantitative volume from the reagent bottle and place it into the test tube.

[0098] Subsequently, the motor drives the active pulley 54 to rotate. Because the active pulley 54 is connected to the driven pulleys 53 on both sides, the driven pulleys 53 drive the test tube support structure 51 to rotate carrying the test tube containing the sample. This process is called the rotation process.

[0099] Since the output shaft of the motor is also fixedly mounted on the rocker arm 52, the rocker arm 52 rotates synchronously, and the synchronous rotation of the rocker arm 52 is a revolution process.

[0100] During the aforementioned rotation and revolution, the sample solution in the test tube support structure 51 is thoroughly mixed.

[0101] Therefore, in order to achieve the linkage between HPLC101 and biosynthesis, this invention improves a novel shaking and mixing mechanism to fully dissolve the sample.

[0102] Example 3

[0103] like Figure 1-10 As shown, in this embodiment, based on the structure of embodiment 2, the test tube support structure 51 includes an upper perforated plate and a lower support plate 512 (with slots for inserting the bottom of the test tube) spaced below the upper perforated plate 511 (which has through holes for cooperating with the test tube). The top center of the lower support plate 512 is fixedly connected to the bottom center of the upper perforated plate 511 by a connecting post.

[0104] Meanwhile, the rotating shaft is fixedly installed at the bottom center of the lower support plate 512; several test tubes 8 are inserted between the upper perforated plate 511 and the lower support plate 512.

[0105] Example 4

[0106] like Figure 1-10 As shown, based on the structure of Example 3, this embodiment, in order to achieve the screw-on fitting for sampling from the small reaction vessel 32 (the screw connection of the vessel lid 321 to the reaction vessel requires a relatively large force to ensure reaction safety, therefore the robot 2 has difficulty screwing on the vessel lid 321 which has a relatively large screw connection force), the specific structure of the moving screw-on fitting assembly 4 is as follows:

[0107] The movable capping assembly 4 includes a gripper cylinder 48, and a capping arm 491 is fixedly installed on each of the two grippers 49 of the gripper cylinder 48 (correspondingly, matching grooves are opened on the side walls of both sides of the lid 321).

[0108] During the cap tightening process, driven by the gripper cylinder 48, the cap clamping arm 491 is engaged in the groove.

[0109] It also includes a U-shaped carriage 46 (U-shaped opening facing upwards); the two sides of the U-shaped carriage 46 are sliding ends, and a U-shaped translation frame 44 (U-shaped opening facing downwards) is slidably connected between the sliding ends.

[0110] The specific sliding structure is as follows: the sliding end has an integrally formed T-shaped sliding seat 461, and T-shaped sliding grooves are opened on the side walls on both sides of the U-shaped translation frame 44, and the two are limited and slid together.

[0111] A rotary motor bracket (i.e., the lower position of the U-shaped carriage 46) is fixedly connected between the sliding ends. A rotary motor 47 is mounted on the top of the rotary motor bracket, and the output end of the rotary motor 47 is mounted on the top of the cylinder of the gripper cylinder 48.

[0112] A lifting cylinder 45 is fixedly connected to the U-shaped translation frame 44, which pushes the gripper cylinder 48 to lift and lower. The pushing end of the lifting cylinder 45 (the piston rod of the lifting cylinder 45 is fixedly mounted on the push rod 43, and the push rod 43 is fixedly mounted on the top of the rotary motor 47) is installed at the top position of the rotary motor 47.

[0113] Meanwhile, since the small reaction vessel 32 is placed horizontally side by side, the above-mentioned mechanism also includes a screw track structure for moving the capping assembly 4. The screw track structure includes a screw track 42, and a U-shaped translation frame 44 is limited and slid at the bottom of the screw track 42.

[0114] Specifically, similar to the existing limit sliding structure, a convex slide block is fixedly installed on the top of the U-shaped translation frame 44, which slides in a groove opened at the bottom of the lead screw track 42. Furthermore, a lead screw (threaded to the slide block) that pushes the U-shaped translation frame 44 to move horizontally is rotatably connected inside the lead screw track 42, and a lead screw motor 41 is installed at the end of the lead screw.

[0115] Similar to existing methods, lead screw bearings that cooperate with the lead screw are installed at both ends of the lead screw track 42, and the two ends of the lead screw are rotatably connected to the lead screw bearings.

[0116] During operation, the screw motor first moves the gripper cylinder 48 to a position directly above the small reaction vessel 32 that needs to be sampled. Then, driven by the lifting cylinder 45, the U-shaped slide 46 carries the rotary motor 47 and the gripper cylinder 48 down until the clamping cover connected to the gripper cylinder 48 is lowered to correspond to the grooves on both sides of the vessel cover 321.

[0117] Subsequently, the gripper cylinder 48 drives the lowering of the vessel lid 321 to grip it, and the rotary motor 47 drives the screwing of the lid. During the screwing process, the lifting cylinder 45 drives the U-shaped slide 46 to move upward, thus continuously rotating and screwing the lid, continuously moving the gripped vessel lid 321 upward until the vessel lid 321 finally comes off.

[0118] The above structure enables the detachment of the threadedly connected kettle lid 321.

[0119] The bottom sides of the aforementioned lead screw track 42 are fixedly installed on the top of the work platform 1 by means of gantry brackets 421.

[0120] Example 5

[0121] like Figure 1-10 As shown, in this embodiment, based on the structure of embodiment 4, after the lid 321 is removed, in order to facilitate the robot 2 to take samples from the small reaction vessel 32, the above-mentioned sampler mechanism includes a pipette assembly and a pipette assembly that cooperates with the pipette assembly.

[0122] Specifically, the pipette assembly includes a pipette holder (with multiple limiting holes), on which several pipettes 7 are placed (the pipettes 7 are inserted into the limiting holes), and the pipette assembly includes a pipette holder, on which several stainless steel long pipettes 71 are placed, and the injection end of the stainless steel long pipettes 71 is integrally formed with a connector for engaging the position of the pipettes 7.

[0123] Specifically, because the small reaction vessel 32 has a certain depth, the robot 2 cannot operate the pipette 7 to extend into the vessel. Furthermore, the plastic pipette 7 is not heat-resistant and can easily contaminate the reaction system.

[0124] Therefore, a stainless steel long pipette 71 (with a thin tube structure for easy and thorough aspiration of materials from the vessel) is used. Similar to existing methods of installing pipettes 7 with thin tubes, a connector 711 is integrally formed at the injection end of the stainless steel long pipette 71 to mate with the pipette 7. The connector 711 is also made of stainless steel. As with existing methods, the connector 711 has a threaded structure on its inner wall. Correspondingly, a threaded structure is formed at the lower end of the pipette 7 to mate with the connector 711, allowing the 20-30cm long stainless steel long pipette 71 to be connected to the lower end of the pipette 7.

[0125] During operation, since it is a dual-arm robot 2, one robotic arm can grasp the pipette 7 and the other robotic arm can grasp the connector and fix it by slightly twisting it.

[0126] Subsequently, robot 2 operates a pipette 7 with a long stainless steel pipette 71 to move to the location of the reaction vessel and operates to aspirate the material in the reaction vessel.

[0127] Then, the movable capping assembly 4 screws the lid 321 back on, completing the sampling process.

[0128] After sampling is completed, robot 2 moves to the location of the test tube assembly and puts the sample into the test tube.

[0129] After adding solvent, place the sample into the mixing and shaking mechanism 5 to dissolve. After dissolution, wait to draw the sample solution from test tube 8 and inject it into the sample vial for injection into HPLC 101. This completes the process from capping, sampling, dissolution, sample preparation, and injection.

[0130] During the operation, robot 2 opens the sample inlet door of HPLC101 and places the sample vial into the sample tray on HPLC101, completing the entire process.

[0131] Example 6

[0132] like Figure 1-10 As shown, based on the structure of Example 5, the sample vial assembly includes a sample vial tray on which several sample vials 10 are placed. The syringe assembly includes a syringe holder and a syringe 6 placed on the syringe holder, which has the same structure as the existing syringe for injecting solution into the sample vial 10. In order to filter insoluble matter in the solution (to prevent clogging of the chromatographic column and causing abnormal column pressure), a filter seat 61 (made of plastic and containing a filter) is installed at the outlet end of the syringe 6.

[0133] Meanwhile, the test tube assembly includes a test tube holder on which test tubes 8 are placed.

[0134] After sampling, the sample is injected into test tube 8. Therefore, the sample needs to be dissolved and diluted. Thus, a reagent bottle holder is fixedly connected to the work platform 1; a reagent bottle 9 is placed on the reagent bottle holder.

[0135] Reagent bottle 9 contains organic and inorganic reagents such as methanol and ethanol. During operation, robot 2 first unscrews the cap on the actual bottle (unlike the reaction vessel cap 321, the cap on reagent bottle 9 is easy to unscrew and can be operated by robot 2), and then the pipette 7 grasped by robot 2 can be used for pipetting.

[0136] Example 7

[0137] like Figure 1-10 As shown, based on the structure of Example 6, this embodiment also discloses the operation method of the above-mentioned intelligent liquid chromatography detection and biosynthesis workstation, including the following steps:

[0138] (1) According to the experimental requirements, put the materials into the small reaction vessel 32 and heat them; during the reaction, take samples once every 1 hour;

[0139] (2) During the heating process, the movable capping assembly 4 is moved to the position of the small reaction vessel 32 and the vessel cap 321 is unscrewed; the specific capping operation is as described in the above embodiment.

[0140] (3) Robot 2 grabs a pipette 7 from the sampler mechanism, installs a stainless steel long pipette 71 at the inlet of the pipette 7, moves the robot 2 to the small reaction vessel to take a sample, and moves the capping assembly 4 to screw the vessel cap 321 on again.

[0141] (4) Robot 2 puts the extracted sample into a test tube, and repeats this process to complete the sampling of all small reaction vessels 32;

[0142] After unscrewing the reagent bottle cap, robot 2 grabs a pipette 7, draws reagent from reagent bottle 9, and puts it into test tube 8 containing the sample;

[0143] (5) Robot 2 picks up the test tube 8 containing the sample and places it on the test tube support structure 51 of the mixing mechanism 5; driven by the motor, the test tube support structure 51 rotates on its own axis and revolves with the rocker 52 to perform double rotation mixing.

[0144] (6) After the mixture is dissolved, the robot 2 grabs the syringe 6 from the syringe assembly to draw up the sample solution and injects it into the sample bottle 10. The sample bottle 10 is then loaded onto the HPLC 101 for detection.

[0145] Example 8

[0146] like Figure 1-10As shown, the working control methods of the robot 2 disclosed in this invention are all conventional methods for controlling existing robots. Meanwhile, the control method of the movable cap-screwing assembly 4 in this invention is also a conventional control method disclosed in the prior art. For example, it is the same as the existing method, such as controlling the gripper cylinder to move precisely to the position above the small reaction vessel 32 by installing a displacement sensor on the movable cap-screwing assembly 4 (similar to the existing method, the displacement sensor can be installed on the U-shaped translation frame 44 to control precise movement and positioning).

[0147] Of course, the above description is not intended to limit the present invention, and the present invention is not limited to the examples given above. Any changes, modifications, additions or substitutions made by those skilled in the art within the scope of the present invention should also fall within the protection scope of the present invention.

Claims

1. A smart liquid chromatography detection and biosynthesis workstation, characterized in that, This includes the work platform and the robot mounted on the work platform for experimental operations; It also includes several biosynthesis mechanisms installed on one side of the top of the work platform, as well as an HPLC; The biosynthesis apparatus includes a heated reaction vessel assembly, which includes a small reaction vessel and a vessel lid threaded onto the reaction vessel; it also includes a movable lid-screwing assembly for unscrewing the vessel lid. It also includes a sampler mechanism for taking samples from a small reactor. After the lid is unscrewed by the moving cap assembly, the robot takes samples from the small reactor through the sampler mechanism. A mixing and shaking mechanism is installed on the other side of the top of the working platform. The mixing and shaking mechanism includes a rocker base, and test tube support structures are rotatably connected to the top two sides of the rocker base. The bottom of the test tube support structure is fixedly connected to a rotating shaft that is rotatably connected to a rocker base, and a driven pulley is installed on the rotating shaft; It also includes a motor mount fixedly installed on the top of the work platform. A motor is mounted on the motor mount, and a drive pulley is fixedly connected to the output shaft of the motor. The drive pulley is connected to the driven pulleys on both sides for transmission. The motor's output shaft is fixedly mounted on the rocker stand; during sample preparation, the test tube support structure rotates on its own axis and revolves around the rocker stand to perform dual rotational mixing. The work platform is equipped with sample bottle components, syringe components, and test tube components to facilitate experimental operations.

2. The intelligent liquid chromatography detection and biosynthesis workstation according to claim 1, characterized in that, The sampler mechanism includes a pipette assembly and a pipette assembly that cooperates with the pipette assembly; The pipette assembly includes a holder on which several pipettes are placed. The pipette assembly includes a pipette rack on which several long stainless steel pipettes are placed. The injection end of the long stainless steel pipettes is integrally formed with a connector for engaging the position of the pipettes.

3. The intelligent liquid chromatography detection and biosynthesis workstation according to claim 1, characterized in that, The sample bottle assembly includes a sample bottle tray on which a plurality of sample bottles are placed; The syringe assembly includes a syringe holder and a syringe placed on the syringe holder, with a filter seat installed at the liquid outlet end of the syringe. The test tube assembly includes a test tube holder on which test tubes are placed.

4. The intelligent liquid chromatography detection and biosynthesis workstation according to claim 1, characterized in that, The movable cap screwing assembly includes a gripper cylinder, and cap clamping arms are fixedly installed on the two grippers of the gripper cylinder respectively. It also includes a U-shaped carriage; the two sides of the U-shaped carriage are sliding ends, and a U-shaped translation frame is slidably connected between the sliding ends; A rotary motor bracket is fixedly connected between the sliding ends. A rotary motor is installed on the top of the rotary motor bracket, and the output end of the rotary motor is installed on the top of the cylinder of the gripper cylinder. A lifting cylinder is fixedly connected to the U-shaped translation frame to push the gripper cylinder to move up and down. The pushing end of the lifting cylinder is installed at the top of the rotary motor.

5. The intelligent liquid chromatography detection and biosynthesis workstation according to claim 4, characterized in that, The movable cap screwing assembly also includes a lead screw track structure, which includes a lead screw track and a U-shaped sliding frame that limits the sliding motion at the bottom of the lead screw track. The lead screw is rotatably connected within the lead screw track to push the U-shaped translation frame to move horizontally, and a lead screw motor is installed at the end of the lead screw. The bottom sides of the lead screw track are fixedly installed on the top of the work platform by gantry brackets.

6. The intelligent liquid chromatography detection and biosynthesis workstation according to claim 1, characterized in that, The test tube support structure includes an upper perforated plate and a lower support plate spaced apart below the upper perforated plate; The rotating shaft is fixedly installed at the bottom center of the lower support plate; Several test tubes are inserted between the upper perforated plate and the lower support plate.

7. The intelligent liquid chromatography detection and biosynthesis workstation according to claim 1, characterized in that, The drive pulley is provided with a pair of drive pulley grooves that are spaced apart vertically. The driven pulleys on both sides are respectively provided with driven pulley grooves that cooperate with the driving pulley grooves; the driving pulley grooves and the corresponding driven pulley grooves are connected by belt drive.

8. The intelligent liquid chromatography detection and biosynthesis workstation according to claim 7, characterized in that, A reagent bottle holder is fixedly connected to the work platform; reagent bottles are placed on the reagent bottle holder.

9. The intelligent liquid chromatography detection and biosynthesis workstation according to claim 8, characterized in that, The heating reactor assembly also includes a heater that works in conjunction with a small reactor; The small reaction vessel can be detachably and fixedly mounted on the heater.

10. An operating method for an intelligent liquid chromatography detection and biosynthesis workstation as described in any one of claims 1-8, characterized in that, Includes the following steps: (1) According to the experimental requirements, put the materials into the small reaction vessel and heat them; (2) During the heating process, the movable capping assembly is moved to the location of the small reaction vessel and the vessel cap is unscrewed; (3) The robot grabs a pipette from the sampler mechanism, installs a long stainless steel pipette at the inlet of the pipette, moves the robot to the small reaction vessel to take a sample, and moves the capping assembly to screw the cap back on. (4) The robot puts the extracted sample into a test tube, and repeats this process to complete the sampling of all small reaction vessels; After the robot unscrews the reagent bottle cap, it grabs a pipette, draws the reagent from the bottle, and puts it into a test tube containing the sample. (5) The robot picks up the test tube containing the sample and places it on the test tube support structure of the mixing mechanism; driven by the motor, the test tube support structure rotates on its own axis and revolves with the rocker to perform double rotation mixing. (6) After the mixture is dissolved, the robot grabs the syringe from the syringe assembly to draw up the sample solution and injects it into the sample bottle. The sample bottle is then loaded onto the HPLC for detection.

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