An improved energy-efficient antibody incubation box

Abstract

The application belongs to the field of incubation equipment, and provides an improved energy-saving and effective antibody incubation box, which comprises a bottom plate, a clamping groove one is opened at the position of the middle line of the top outer wall of the bottom plate, a plurality of clamping grooves two are respectively opened at the two sides of the clamping groove one close to the top outer wall of the bottom plate, the depth of the clamping groove one is greater than the depth of the clamping grooves two on the two sides, a rubber pad is bonded on the inner wall of the clamping groove one, an incubation box body is clamped on the inner wall of the rubber pad, a plurality of water solution box bodies are respectively clamped on the inner walls of the corresponding clamping grooves two, the height of the incubation box body is lower than the height of the water solution box body, and a liquid collecting mechanism is installed on the top of the incubation box body.

Description

Technical Field

[0001] This invention belongs to the field of incubation equipment, specifically an improved, energy-efficient antibody incubation box. Background Technology

[0002] Currently, there are two main methods for antibody incubation after Western blot transfer: artificial wet chambers and commercially available antibody incubation kits. Artificial wet chambers can lead to overnight antibody incubation, causing the diluted antibody to evaporate and resulting in unnecessary losses. Furthermore, commercially available antibody incubation kits are not meticulously manufactured, often only considering the length and width of the NC membrane, resulting in kits with a height greater than 1 cm. This height is significant relative to the micrometer-thickness of the membrane itself, requiring a large amount of diluted antibody and causing losses during recovery and in the antibody's shelf life after dilution, thus wasting experimental costs.

[0003] Current antibody incubation kits do not adequately consider the thickness of the NC membrane after transfer. They are designed simply based on the approximate length and width of the membrane, resulting in incubation kits that are tall and long and wide enough. This leads to the requirement for a high antibody concentration, and the antibody will evaporate during overnight incubation, resulting in a loss of antibody concentration during recovery and waste.

[0004] To address these issues, those skilled in the art have proposed an improved, energy-efficient, and effective antibody incubation box. Summary of the Invention

[0005] To address the aforementioned technical problems, this invention provides an improved, energy-efficient antibody incubation box to solve the problem of antibody content loss during recovery after overnight incubation and evaporation in the prior art.

[0006] An improved, energy-efficient antibody incubation kit, comprising:

[0007] The base plate has a slot 1 at the center line of the top outer wall, and multiple slots 2 are respectively opened on both sides of the top outer wall of the base plate near the slot 1. The depth of the slot 1 is greater than the depth of the slots 2 on both sides.

[0008] The incubation box body has a rubber pad adhered to the inner wall of the slot one, and the incubation box body is snapped onto the inner wall of the rubber pad;

[0009] An aqueous solution box, wherein multiple aqueous solution boxes are respectively snapped onto the inner wall of a corresponding slot two, and the height of the incubation box is lower than the height of the aqueous solution box;

[0010] A liquid-gathering mechanism is installed on the top of the incubation chamber and is used for reflux after antibody evaporation.

[0011] A first covering mechanism is located on the outside of the aqueous solution container;

[0012] The second covering mechanism is installed on the surface of the first covering mechanism, and the base plate, the first covering mechanism, and the second covering mechanism are combined to form a closed space.

[0013] Preferably, L-shaped clamping plates are fixedly installed on the front and rear outer walls of the base plate near both ends, and rectangular grooves are opened on both sides of the top outer wall of the base plate, with the rectangular grooves located between the corresponding two L-shaped clamping plates.

[0014] Preferably, the covering mechanism includes a rectangular card frame, the bottom of which is engaged with the inner wall of the frame composed of a rectangular groove and an L-shaped card plate, and both ends of the top outer wall of the rectangular card frame are provided with limit grooves.

[0015] Preferably, the second covering mechanism includes a cover plate, with fixing plates fixedly installed at both ends of the bottom outer wall of the cover plate. The fixing plates are snapped onto the inner wall of the corresponding limiting groove. An installation opening is opened at the center of the top outer wall of the cover plate, and an observation window is installed on the inner wall of the installation opening. Handles are installed on both sides of the top outer wall of the cover plate. The enclosed space formed by the first covering mechanism and the second covering mechanism contains the incubation box and the aqueous solution box inside.

[0016] Preferably, a pad block is fixed to the top outer wall of the rubber pad near the rear end of the base plate, and the longitudinal section of the pad block is triangular.

[0017] Preferably, one corner of the bottom outer wall of the incubation box is attached to the inclined surface of the pad, the longitudinal section of the incubation box is the same as the shape of the NC film, and the width of the incubation box is 1 to 2 mm.

[0018] Preferably, the liquid-gathering mechanism includes a top plate, with handles installed on both sides of the top outer wall of the top plate, and a sealing plate installed on the bottom outer wall of the top plate. The sealing plate is snapped into the inner wall of the incubation box near the top, and the top plate is attached to the surface of the incubation box.

[0019] Preferably, a liquid-gathering groove is formed at the center line of the bottom outer wall of the sealing plate, the depth of the liquid-gathering groove becomes shallower from the front end to the rear end, and a first inclined groove is formed on both sides of the front end of the sealing plate near the liquid-gathering groove, and a second inclined groove is formed on both sides of the rear end of the sealing plate near the liquid-gathering groove, the inclination of the first inclined groove is less than the inclination of the second inclined groove.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] 1. The present invention provides an incubation box with a thickness approximately the same as that of the NC membrane. During incubation, the NC membrane is placed vertically inside the incubation box using tweezers. The incubation box occupies little space, which reduces the amount of diluted antibody required and minimizes antibody loss during recovery and after dilution, thereby saving costs.

[0022] 2. This invention includes a liquid-gathering mechanism, a first covering mechanism, and a second covering mechanism. The bottom of the liquid-gathering mechanism is provided with a liquid-gathering tank, a first inclined tank, and a second inclined tank. When the antibody in the incubation box is incubated overnight, it evaporates and condenses on the surface of the sealing plate. Then, under the action of gravity, the liquid near the front end of the liquid-gathering tank, the first inclined tank, and the second inclined tank converge towards the rear end of the liquid-gathering tank and then flow back to the bottom, reducing antibody loss. In the closed space formed by the first covering mechanism and the second covering mechanism, the aqueous solution box contains clean water. Within one space, the evaporation of clean water can reduce the volatilization of the antibody inside the incubation box, thereby achieving the effect of reducing antibody loss. Attached Figure Description

[0023] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0024] Figure 2 This is a schematic diagram of the structure of the first and second covering mechanisms of the present invention;

[0025] Figure 3 This is a schematic diagram of the base plate structure of the present invention;

[0026] Figure 4 This is a partial three-dimensional structural diagram of the present invention;

[0027] Figure 5 This is a schematic diagram of the incubation box structure of the present invention;

[0028] Figure 6 This is a schematic diagram of the polymer liquid mechanism structure of the present invention;

[0029] Figure 7 for Figure 6 Another structural diagram from another angle.

[0030] In the picture:

[0031] 1. Base plate; 2. L-shaped card plate; 3. Covering mechanism one; 4. Covering mechanism two; 5. Card slot one; 6. Card slot two; 7. Rubber pad; 8. Rectangular groove; 9. Pad block; 10. Incubation box body; 11. Aqueous solution box body; 12. Liquid collection mechanism;

[0032] 301. Rectangular card frame; 302. Limiting groove;

[0033] 401. Cover plate; 402. Handle; 403. Observation window; 404. Fixing plate;

[0034] 1201. Top plate; 1202. Handle; 1203. Sealing plate; 1204. Inclined groove one; 1205. Liquid collection tank; 1206. Inclined groove two. Detailed Implementation

[0035] The embodiments of the present invention will be described in further detail below with reference to the accompanying drawings and examples. The following examples are for illustrative purposes only and should not be construed as limiting the scope of the invention.

[0036] As attached Figure 1 To be continued Figure 7 As shown:

[0037] This invention provides an improved, energy-efficient antibody incubation box, comprising:

[0038] The base plate 1 has a slot 5 at the center line of the top outer wall. Multiple slots 6 are opened on both sides of the top outer wall of the base plate 1 near the slot 5. The depth of the slot 5 is greater than the depth of the slots 6 on both sides.

[0039] A rubber pad 7 is adhered to the inner wall of the incubation box 10 and the slot 5, and the incubation box 10 is snapped onto the inner wall of the rubber pad 7.

[0040] Aqueous solution box 11, multiple aqueous solution boxes 11 are respectively snapped into the inner wall of the corresponding slot 2 6, and the height of the incubation box 10 is lower than the height of the aqueous solution box 11;

[0041] Liquid collection mechanism 12 is installed on top of incubation box 10 and is used for reflux after antibody evaporation;

[0042] Covering mechanism 3 is located on the outside of the aqueous solution box 11;

[0043] Covering mechanism 2 4 is installed on the surface of covering mechanism 1 3. The base plate 1, covering mechanism 1 3 and covering mechanism 2 4 are combined to form a closed space.

[0044] As can be seen from the above, when using the device, remove the second covering mechanism 4, take out the first covering mechanism 3, open the liquid collection mechanism 12, pour the antibody dilution solution into the incubation box 10, then use tweezers to vertically place the NC membrane into the incubation box 10, pour clean water into the aqueous solution box 11, and then cover the first covering mechanism 3 and the second covering mechanism 4 to carry out overnight antibody incubation. This takes advantage of space and achieves the purpose of saving antibody content and experimental costs.

[0045] Specifically, L-shaped clamping plates 2 are fixedly installed on the front and rear outer walls of the base plate 1 near both ends, and rectangular grooves 8 are opened on both sides of the top outer wall of the base plate 1, with the rectangular grooves 8 located between the corresponding two L-shaped clamping plates 2.

[0046] As can be seen from the above, the L-shaped card plate 2 and the rectangular groove 8 facilitate the locking of the covering mechanism 3, making it easier to form a closed space and facilitate the experiment.

[0047] Specifically, the covering mechanism 3 includes a rectangular card frame 301. The bottom of the rectangular card frame 301 is engaged with the inner wall of the frame formed by the rectangular groove 8 and the L-shaped card plate 2. Limiting grooves 302 are opened at both ends of the top outer wall of the rectangular card frame 301.

[0048] As can be seen from the above, the incubation box 10 and the aqueous solution box 11 are contained inside by the rectangular card frame 301.

[0049] Specifically, the second covering mechanism 4 includes a cover plate 401. Both ends of the bottom outer wall of the cover plate 401 are fixedly installed with fixing plates 404. The fixing plates 404 are snapped into the inner wall of the corresponding limiting groove 302. An installation opening is opened at the center of the top outer wall of the cover plate 401. An observation window 403 is installed on the inner wall of the installation opening. Handles 402 are installed on both sides of the top outer wall of the cover plate 401. The enclosed space formed by the first covering mechanism 3 and the second covering mechanism 4 contains the incubation box 10 and the aqueous solution box 11 inside.

[0050] As can be seen from the above, the observation window 403 facilitates the observation of the antibody incubation experiment, and the cover plate 401, together with the rectangular card frame 301 and the base plate 1, forms a closed space.

[0051] Specifically, a pad 9 is fixed to the top outer wall of the rubber pad 7 near the rear end of the base plate 1, and the longitudinal section of the pad 9 is triangular.

[0052] As can be seen from the above, the incubation box 10 is wrapped with rubber pad 7, which makes the incubation box 10 more stable, and the incubation box 10 is supported by pad block 9.

[0053] Specifically, one corner of the bottom outer wall of the incubation box 10 is attached to the inclined surface of the pad 9, the longitudinal section of the incubation box 10 is the same as the shape of the NC membrane, and the width of the incubation box 10 is 1 to 2 mm.

[0054] As can be seen from the above, the incubation box 10 allows for the vertical placement of the NC membrane, reducing the use of antibody dilution solution.

[0055] Specifically, the liquid collection mechanism 12 includes a top plate 1201, handles 1202 are installed on both sides of the top outer wall of the top plate 1201, and a sealing plate 1203 is installed on the bottom outer wall of the top plate 1201. The sealing plate 1203 is snapped into the inner wall of the incubation box 10 near the top, and the top plate 1201 is attached to the surface of the incubation box 10.

[0056] As can be seen from the above, the incubation box 10 is sealed by the top plate 1201 and the sealing plate 1203, which reduces the volatilization of antibodies during the experiment.

[0057] Specifically, a liquid-gathering groove 1205 is opened at the center line of the bottom outer wall of the sealing plate 1203. The depth of the liquid-gathering groove 1205 becomes shallower from the front end to the rear end. Inclined groove 1204 is opened on both sides of the front end of the sealing plate 1203 near the liquid-gathering groove 1205, and inclined groove 2 1206 is opened on both sides of the rear end of the sealing plate 1203 near the liquid-gathering groove 1205. The inclination of inclined groove 1204 is less than that of inclined groove 2 1206.

[0058] As can be seen from the above, the set liquid collection tank 1205, inclined groove one 1204 and inclined groove two 1206 facilitate the aggregation and reflux of antibodies after evaporation. When the antibodies in the incubation box 10 are incubated overnight, they are condensed on the surface of the sealing plate 1203 after evaporation. Then, under the action of gravity, the liquid near the front end of the liquid collection tank 1205, the position of inclined groove one 1204 and inclined groove two 1206 converge towards the rear end of the liquid collection tank 1205 and then flow back to the bottom, reducing the loss of antibodies.

[0059] In use, multiple devices are taken out, one device per group. Multiple groups are used to conduct experiments to enhance the accuracy of the experiment. Remove the second cover mechanism 4, take out the first cover mechanism 3, open the liquid collection mechanism 12, pour the antibody dilution into the incubation box 10, and then use tweezers to vertically place the NC membrane into the incubation box 10. Pour clean water into the aqueous solution box 11, and then cover the first cover mechanism 3 and the second cover mechanism 4. Incubate the antibody overnight to take advantage of space and save antibody content and experimental costs. When the antibody is incubated overnight in the incubation box 10, it evaporates and condenses on the surface of the sealing plate 1203. Then, under the action of gravity, the liquid near the front end of the liquid collection tank 1205, the first inclined groove 1204 and the second inclined groove 1206 converge towards the rear end of the liquid collection tank 1205 and then flow back to the bottom, reducing antibody loss.

[0060] The embodiments of the present invention are given for the purposes of illustration and description. Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of the present invention.

Claims

1. An improved, energy-efficient, and effective antibody incubation box, characterized in that, include: The base plate (1) has a slot 1 (5) at the center line of the top outer wall of the base plate (1), and multiple slots 2 (6) are opened on both sides of the top outer wall of the base plate (1) near the slot 1 (5). The depth of the slot 1 (5) is greater than the depth of the slots 2 (6) on both sides. The incubation box (10) has a rubber pad (7) glued to the inner wall of the slot (5), and the incubation box (10) is snapped onto the inner wall of the rubber pad (7). Aqueous solution box (11), multiple aqueous solution boxes (11) are respectively snapped onto the inner wall of the corresponding slot two (6), and the height of the incubation box (10) is lower than the height of the aqueous solution box (11); A liquid-gathering mechanism (12) is installed on top of the incubation box (10) and is used for reflux after antibody evaporation; A covering mechanism (3) is located on the outside of the aqueous solution container (11); The second covering mechanism (4) is installed on the surface of the first covering mechanism (3). The base plate (1), the first covering mechanism (3) and the second covering mechanism (4) are combined to form a closed space.

2. The improved energy-saving and efficient antibody incubation box according to claim 1, characterized in that: L-shaped clamping plates (2) are fixedly installed on the front and rear outer walls of the base plate (1) near both ends. Rectangular grooves (8) are opened on both sides of the top outer wall of the base plate (1), and the rectangular grooves (8) are located between the corresponding two L-shaped clamping plates (2).

3. The improved energy-saving and efficient antibody incubation box according to claim 2, characterized in that: The covering mechanism (3) includes a rectangular card frame (301), the bottom of which is engaged with the inner wall of the frame formed by the rectangular groove (8) and the L-shaped card plate (2), and the two ends of the top outer wall of the rectangular card frame (301) are provided with limit grooves (302).

4. The improved energy-saving and efficient antibody incubation box according to claim 3, characterized in that: The second covering mechanism (4) includes a cover plate (401). Both ends of the bottom outer wall of the cover plate (401) are fixedly installed with fixing plates (404). The fixing plates (404) are snapped into the inner wall of the corresponding limiting groove (302). An installation opening is opened at the center of the top outer wall of the cover plate (401). An observation window (403) is installed on the inner wall of the installation opening. Handles (402) are installed on both sides of the top outer wall of the cover plate (401). The enclosed space formed by the first covering mechanism (3) and the second covering mechanism (4) contains the incubation box (10) and the aqueous solution box (11) inside.

5. The improved energy-saving and efficient antibody incubation box according to claim 1, characterized in that: A pad block (9) is fixed on the top outer wall of the rubber pad (7) near the rear end of the bottom plate (1). The longitudinal section of the pad block (9) is triangular.

6. The improved energy-saving and efficient antibody incubation box according to claim 3, characterized in that: One corner of the bottom outer wall of the incubation box (10) is attached to the inclined surface of the pad (9), and the longitudinal section of the incubation box (10) is the same as the shape of the NC membrane.

7. The improved energy-saving and efficient antibody incubation box according to claim 1, characterized in that: The liquid-gathering mechanism (12) includes a top plate (1201), with handles (1202) installed on both sides of the top outer wall of the top plate (1201), and a sealing plate (1203) installed on the bottom outer wall of the top plate (1201). The sealing plate (1203) is snapped into the inner wall of the incubation box (10) near the top, and the top plate (1201) is attached to the surface of the incubation box (10).

8. The improved energy-efficient antibody incubation box according to claim 7, characterized in that: A liquid-gathering groove (1205) is provided at the center line of the bottom outer wall of the sealing plate (1203). The depth of the liquid-gathering groove (1205) decreases from the front end to the rear end. An inclined groove 1 (1204) is provided on both sides of the front end of the sealing plate (1203) near the liquid-gathering groove (1205). An inclined groove 2 (1206) is provided on both sides of the rear end of the sealing plate (1203) near the liquid-gathering groove (1205). The inclination of the inclined groove 1 (1204) is less than that of the inclined groove 2 (1206).

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