A method of preventing lysine feed additive caking

Abstract

The present disclosure relates to a method for preventing caking of lysine feed additive. Specifically, the present disclosure relates to a composition for preventing caking of lysine feed additive, which is capable of inhibiting caking of lysine feed additive and improving storage stability, and a method for preventing caking of lysine feed additive using the same.

Description

Technical Field

[0001] This disclosure relates to a method for preventing lysine feed additives from caking. Specifically, this disclosure relates to a composition for preventing lysine feed additive caking, the composition being able to inhibit lysine feed additive caking and improve storage stability, and a method for using the composition to prevent lysine feed additive caking. Background Technology

[0002] One problem that can occur during the storage and transportation of manufactured products is clumping. Clumping is influenced by external factors such as moisture, temperature, pressure / stress, mixing, electric field, and time, as well as by intrinsic factors of the powder particles, such as chemical composition, molecular weight, surface charge / polarity, physical state, size, and shape (linear / plate-like / spherical). These factors affect various reactions (e.g., hydration, dissolution, deliquescence, and decomposition), phase transitions (e.g., glass transition to rubbery state, recrystallization, and solidification), and particle redistribution (e.g., abrasion and breakage, and particle segregation) during processes such as heating or cooling, hygroscopic or drying, pressurization, and handling. As a result, the viscosity of the particle surface increases, and liquid / solid bridges are formed, leading to clumping through sintering and interlocking. Liquid / solid bridges between powder particles form due to various reasons, such as moisture adsorption and drying, lipid melting and solidification, solute dissolution and drying, and crystallization. Food Research International (Volume 45, Issue 1, January 2012, pp. 369-380). Agglomeration occurs for the various reasons mentioned above and is an industrial challenge that needs to be addressed.

[0003] Meanwhile, lysine carries a cationic charge in the absence of counterions, and therefore exhibits very high reactivity with moisture. This increases hygroscopicity and leads to clumping. To prevent this, counterions are provided to lysine, such as hydrochloric acid, sulfuric acid, or carbonic acid. However, even with the reduction of hygroscopicity by providing such counterions, particle aggregation and clumping can still occur during long-term storage or transportation.

[0004] Therefore, for feed additives containing lysine, there is a need for a preparation method that can inhibit clumping and improve storage stability. Summary of the Invention

[0005] [Technical Issues] This disclosure aims to provide a composition for preventing lysine feed additives from caking, the composition being able to inhibit lysine feed additive caking and improve storage stability, and a method for using the composition to prevent lysine feed additive caking.

[0006] [Technical Solution] One object of this disclosure is to provide a composition for preventing caking of lysine feed additives, comprising calcium stearate.

[0007] Another object of this disclosure is to provide a method for preventing lysine feed additives from caking using the composition of this disclosure for preventing lysine feed additive caking.

[0008] [Beneficial Effects] The compositions and methods disclosed herein for preventing caking inhibit caking and improve storage stability by adding an anti-caking agent to a lysine feed additive with high hygroscopicity, and can also provide a lysine feed additive with high lysine content and minimal amount of anti-caking agent. Detailed Implementation

[0009] This disclosure will now be described in detail. Furthermore, each description and embodiment disclosed herein can also be applied to other descriptions and embodiments. That is, all combinations of the various elements disclosed herein fall within the scope of this disclosure. Moreover, the scope of this disclosure is not limited to the specific descriptions below.

[0010] To achieve the above objectives, one aspect of this disclosure provides a composition for preventing lysine carbonate from agglomerating, comprising calcium stearate.

[0011] The composition disclosed herein for preventing caking can contain calcium stearate in lysine feed additives to solve the caking problem of lysine products in the feed additive industry, and the method disclosed herein for preventing caking can inhibit caking by adding and mixing calcium stearate in powder form.

[0012] As used herein, the term "calcium stearate" refers to calcium carboxylate, which is a calcium salt with the chemical formula C that can be added to food. 36 H 70 Compounds of CaO4. When calcium stearate is added to lysine feed additives, the agglomeration of lysine, which exhibits very high reactivity with moisture, is inhibited, thereby improving agglomeration and enhancing storage stability.

[0013] Lysine feed additives refer to feed additives containing lysine, and are typically provided in the form of salts by providing counterions. Specifically, lysine feed additives can be lysine hydrochloride, lysine sulfate, or lysine carbonate, more specifically lysine carbonate. In this case, the counterions of lysine are provided, thereby reducing reactivity with moisture and decreasing hygroscopicity. Furthermore, lysine feed additives can be in powder or granular form suitable for use as feed additives.

[0014] In lysine carbonate, the lysine content can be from 70 wt% to 85 wt%, specifically from 76 wt% to 80 wt%.

[0015] Lysine carbonate can be prepared by fermenting a lysine-producing strain (e.g., a lysine-glutamate-producing Corynebacterium strain) to obtain an L-lysine fermentation broth, and then injecting carbon dioxide into the fermentation broth. Therefore, it may contain carbonate or bicarbonate ions. The carbonate or bicarbonate content can be from 2 wt% to 23 wt%, specifically 2 wt% to 19 wt%, 3 wt% to 22 wt%, 3 wt% to 18 wt%, or 6 wt% to 15 wt%.

[0016] Lysine carbonate can have a pH of 8 to 10, specifically 8.5 to 9.5.

[0017] Another aspect of this disclosure provides a method for preventing lysine carbonate particles from clumping, comprising adding calcium stearate to the lysine carbonate particles.

[0018] Specifically, calcium stearate and the prevention of clumping are as described above.

[0019] Calcium stearate can be added in powder form.

[0020] Among various commercially available lysine feed additive formulations and types, the calcium stearate of this invention exhibits particularly superior anti-caking and anti-caking effects compared to lysine carbonate granules.

[0021] Even when added in small amounts compared to lysine carbonate particles, calcium stearate exhibits excellent anti-caking and anti-caking effects. Specifically, when added in powder form, calcium stearate may be added in an amount of 0.1% (w / w) to 0.5% (w / w) relative to the lysine carbonate particles, more specifically, in amounts of 0.2% (w / w) to 0.5% (w / w), 0.25% (w / w) to 0.5% (w / w), 0.1% (w / w) to 0.4% (w / w), 0.1% (w / w) to 0.3% (w / w), 0.2% (w / w) to 0.4% (w / w), 0.2% (w / w) to 0.3% (w / w), 0.2% (w / w) to 0.25% (w / w), 0.25% (w / w) to 0.3% (w / w), or 0.25% (w / w).

[0022] In addition, calcium stearate may be added in an amount of 0.1% (w / w) to 0.7% (w / w) of lysine in lysine carbonate, and specifically in amounts of 0.1% (w / w) to 0.6% (w / w), 0.2% (w / w) to 0.5% (w / w), 0.3% (w / w) to 0.4% (w / w), or 0.3% (w / w) to 0.35% (w / w).

[0023] In addition, calcium stearate may be added in an amount of 0.5% (w / w) to 10% (w / w) relative to the carbonate or bicarbonate in lysine carbonate, and specifically in an amount of 0.6% (w / w) to 8.5% (w / w) or 1% (w / w) to 5% (w / w).

[0024] In this case, even using a small amount of anti-caking agent can achieve excellent caking inhibition effect, reduce costs, and maintain high lysine content in lysine feed additives, especially lysine carbonate.

[0025] Methods for preventing agglomeration may have an agglomeration reduction rate of at least 50%, particularly a combination of a lower limit selected from 55%, 60%, 64%, 70%, 80% or 85% and an upper limit selected from 100%, 95%, 90%, 86% or 85%.

[0026] As used herein, the term “agglomeration” refers to the amount of agglomerates formed by clumping in a powder, and the term “agglomeration reduction rate” refers to the degree to which the amount of such agglomerates is reduced relative to the absence of an anti-caking agent.

[0027] The measurement of agglomeration can be achieved by separating agglomerated agglomerates from powder and measuring their mass, or by storing the powder under conditions that accelerate agglomeration. For example, powder with added anti-caking agents can be stored in a constant temperature and humidity chamber (30 °C, 60% relative humidity) for 24 hours, then compressed (7 bar, 10 minutes) to accelerate agglomeration, after which the agglomerated agglomerates are separated by sieve and weighed.

[0028] After implementing methods to prevent agglomeration, the deagglomeration rate can be at least 90%, specifically at least 95%, at least 98%, or at least 99%.

[0029] As used in this article, the term "deagglomeration rate" refers to the degree to which the agglomeration state of aggregates formed by agglomeration is released through external stimuli, such as vibration, crushing, sieving, etc.

[0030] The measurement of deagglomeration rate can be achieved by separating agglomerated agglomerates from powder and measuring their deagglomeration upon application of an external stimulus, and any conventional method can be used without limitation. For example, agglomerated agglomerates can be separated from a sample by a sieve, and vibration can be applied to them to measure the degree of deagglomeration.

[0031] [Methods of Implementing the Invention] The present disclosure will be described in more detail below by way of exemplary embodiments. However, the following exemplary embodiments are merely preferred embodiments for illustrating the present disclosure and are therefore not intended to limit the scope of the disclosure thereto. Meanwhile, technical matters not described in this specification can be fully understood and readily implemented by those skilled in the art or similar art.

[0032] Example: Evaluation of caking in lysine feed additives based on the addition of anti-caking agent powder To optimize the anti-caking agent and its content suitable for lysine feed additives, agglomeration was evaluated when the anti-caking agent powder was mixed with commercially produced lysine sulfate granules (CJ CheilJedang, South Korea) and lysine carbonate granules produced based on the published methods (Korean Patent Application No. KR10-2020-0073298A and US Patent Application No. US2022-0030914A1).

[0033] Specifically, for lysine carbonate particles, an L-lysine fermentation broth is obtained by fermenting a lysine-producing Corynebacterium glutamicum strain, followed by cell removal to obtain an L-lysine aqueous solution. Carbon dioxide is injected into the L-lysine aqueous solution to contain carbonate ions, and lysine carbonate particles are prepared by spray granulation.

[0034] The lysine carbonate particles contain 76 wt% to 80 wt% lysine, 6 wt% to 15 wt% bicarbonate, and have a pH of 8.5 to 9.5.

[0035] To optimize the anti-caking agent and its content for use in lysine feed additives, caking was evaluated when anti-caking agent powder was mixed with commercially produced lysine feed additives.

[0036] First, in this embodiment, two types of lysine feed additives were used: lysine sulfate granules (CJ CheilJedang, South Korea) and lysine carbonate granules (CJ CheilJedang, South Korea).

[0037] Example 1: Confirmation of the anti-caking effect based on the type of lysine feed additive First, calcium stearate (JUNSEI CHEMICAL) was used as an anti-caking agent, and the anti-caking effect of adding and mixing the anti-caking agent was evaluated for each lysine feed additive.

[0038] Specifically, place 140 g of lysine carbonate granules or lysine sulfate granules into a 500 mL PE bottle, add 0.25% (w / w) or 0.5% (w / w) of calcium stearate powder relative to the product weight, and seal the bottle. Then connect the PE bottle containing the lysine feed additive product and anti-caking agent to a stirrer (Daihan Scientific, HT-50AX) and mix at 40 rpm for 10 minutes.

[0039] Subsequently, to evaluate the agglomeration of lysine carbonate and lysine sulfate particles with and without anti-caking agent powder, 140 g of each product, mixed with and without anti-caking agent powder, was placed in a constant temperature and humidity chamber (TH3-E-200, JeioTech Co.) at 30°C and 60% RH. After 24 hours, the moisture-absorbing product was placed in a PE bag (14 cm × 14 cm) and compressed at 7 bar for 10 minutes. The compressed sample was placed on a sieve (12 mesh, Daihan Scientific), the PE bag was removed, and the agglomeration rate (agglomeration rate) was measured. Based on the agglomeration rate obtained without anti-caking agent, the agglomeration reduction rate with anti-caking agent was calculated. Then, the sieve containing the product was transferred to a vibrating device (Minoe 200, Endecotts), vibrated at 50 Hz for 5 minutes, and the deagglomeration rate (breakage rate) was measured.

[0040] [Table 1] As shown in Table 1 above, the results confirmed that even with the addition of only a small amount (approximately 0.25 wt%) of calcium stearate, lysine carbonate granules exhibited a significant reduction in agglomeration compared to lysine sulfate granules. Furthermore, the deagglomeration rate of lysine carbonate granules was confirmed to be 100%. These results demonstrate that when calcium stearate is added as an anti-caking agent in powder form to lysine carbonate granules, a small amount of anti-caking agent can achieve excellent anti-caking effects while maintaining a high lysine content in the lysine feed additive.

[0041] Example 2: Confirming the anti-caking effect based on the type of anti-caking agent Example 1 above demonstrates the excellent anti-caking effect of calcium stearate as an anti-caking agent for lysine carbonate particles, and therefore compares it with other anti-caking agents.

[0042] In addition to calcium stearate, silica (DAEJUNG), ground silica (DAEJUNG), and calcium phosphate (DAEJUNG) were also used as anti-caking agents.

[0043] The methods for adding the anti-caking agent and measuring the agglomeration reduction rate and deagglomeration rate are the same as in Example 1.

[0044] [Table 2] As shown in Table 2 above, the results confirm that when calcium stearate is used as an anti-caking agent, the agglomeration reduction rate is significantly higher compared to other anti-caking agents. Simultaneously, all anti-caking agents exhibited excellent deagglomeration rates. These results demonstrate that when calcium stearate is added to lysine carbonate particles in powder form as an anti-caking agent, it achieves superior agglomeration inhibition effects compared to other anti-caking agents.

[0045] In summary, this demonstrates that the combination of lysine carbonate granules as a lysine feed additive and calcium stearate as an anti-caking agent is an excellent combination for inhibiting agglomeration.

[0046] Based on the foregoing description, those skilled in the art will understand that this disclosure can be implemented in other specific forms without modifying its technical concept or essential features. In this regard, the above embodiments should be understood as illustrative in every respect, not restrictive. The scope of this disclosure should be interpreted as the meaning and scope of the appended claims, not the specific embodiments, and all changes or variations derived from equivalent concepts fall within the scope of this disclosure.

Claims

1. A method for preventing lysine carbonate particles from clumping, comprising adding calcium stearate to the lysine carbonate particles.

2. The method according to claim 1, wherein the calcium stearate is added in powder form.

3. The method of claim 1, wherein the calcium stearate is added in an amount of 0.1% to 0.5% (w / w) relative to the lysine carbonate particles.

4. The method of claim 1, wherein the agglomeration reduction rate is at least 50% compared to lysine carbonate particles without added calcium stearate.

5. The method of claim 1, wherein the depolymerization rate is at least 90%.

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