Microfluidic device, connection device, and liquid chromatograph

Abstract

A microfluidic device has a body portion composed of one or more components, the body portion including a connection portion connectable to the separation column and a plurality of flow paths including a sample holding portion holding a sample and an introduction flow path capable of introducing the sample to the separation column, and further has a switching component slidably connected to the body portion and capable of switching the connection of the plurality of flow paths by sliding with respect to the body portion to selectively connect.

Description

Technical Field

[0001] This invention relates to microfluidic devices, connecting devices, and liquid chromatographs. Background Technology

[0002] In conventional liquid chromatographs, piping is used to connect modules. This piping has an internal capacity. When the internal capacity of the piping from the pump to the separation column is large, a gradient delay occurs (the delay until the change in the composition of the mobile phase generated by the pump reaches the separation column). Furthermore, when the internal capacity of the piping from the autosampler to the detector is large, the sample diffuses within the mobile phase (hereinafter referred to as extra-column diffusion). When the sample diffuses excessively within the mobile phase, the separation accuracy of compounds in the sample within the separation column decreases. In particular, extra-column diffusion in nanoliter / microliter flow rate analysis can have a significant impact on the analytical results. Patent Document 1 describes a microfluidic device that suppresses the increase in internal capacity by integrally forming the sample holding section of the autosampler, the separation column, and the detector connection section on a chip.

[0003] Patent Document 1: US Patent No. 6,958,119 Summary of the Invention

[0004] The problem the invention aims to solve

[0005] In microfluidic devices, components sometimes need to be replaced to meet analytical conditions. In the microfluidic device described in Patent Document 1, since the sample holding section, separation column, and detector connection of the autosampler are integrally formed, individual components cannot be replaced independently. Therefore, when any component integrally formed within the microfluidic device fails to meet the analytical conditions, the microfluidic device itself needs to be replaced. For example, since the packing material of the separation column is fixed to the chip, the separation mode and column length are limited. When changing the separation mode or column length, a microfluidic device with a separation column including the desired separation mode or column length needs to be adopted. Furthermore, since detectors have different connection shapes depending on their type, a corresponding microfluidic device needs to be adopted depending on the detector used.

[0006] The purpose of this invention is to provide a microfluidic device, a connecting device, and a liquid chromatograph that can improve the convenience of sample analysis while improving the accuracy of sample analysis.

[0007] Technical solutions to the problem

[0008] A first aspect of the present invention relates to a microfluidic device comprising a body portion consisting of one or more components, the body portion including a connection portion capable of being connected to a separation column and a plurality of flow paths, the plurality of flow paths including a sample holding portion for holding a sample and an introduction flow path capable of introducing a sample into the separation column for separating the sample, the microfluidic device further comprising a switching component slidably connected to the body portion and capable of selectively connecting the plurality of flow paths by sliding relative to the body portion.

[0009] A second aspect of the present invention relates to a connecting device for connecting the aforementioned microfluidic device, the separation column, and the detector, comprising: a first fixing member capable of fixing the microfluidic device and connecting the connecting portion of the microfluidic device to the inlet port of the separation column; a second fixing member capable of fixing the detector and connecting to the inlet port of the detector and the outlet port of the separation column; a guide rail configured to allow at least one of the first fixing member and the second fixing member to move relative to the other; and a clamping mechanism capable of reducing the distance between the first fixing member and the second fixing member disposed on the guide rail.

[0010] A third aspect of the present invention relates to a liquid chromatograph comprising: the aforementioned microfluidic device, wherein the plurality of flow paths further includes a first port connected to a pump for discharging a mobile phase and a second port connected to a sample filling section for filling a sample; the switching component is capable of switching to: a first state connecting the second port and the sample holding section, and a second state connecting the first port, the sample holding section, and the inlet flow path; the pump; the sample filling section; the separation column; and a detection section for detecting components that have passed through the separation column.

[0011] A fourth aspect of the present invention relates to a microfluidic device comprising: a body portion having a plurality of flow paths, and a switching member slidably connected to the body portion and capable of selectively connecting the plurality of flow paths of the body portion by sliding relative to the body portion, the body portion comprising: a first plate-shaped member and a second plate-shaped member, the plurality of flow paths comprising: a sample holding flow path formed in the first plate-shaped member and holding a sample, and a chromatographic column flow path formed in the second plate-shaped member and capable of separating the sample by components.

[0012] A fifth aspect of the present invention relates to a liquid chromatograph comprising: the microfluidic device described above, wherein the body further comprises a third plate-shaped component, the third plate-shaped component including a connecting portion, the connecting portion being connectable to a detection unit for detecting components passing through the chromatographic column flow path, the plurality of flow paths including: a first port connected to a pump for discharging mobile phase, a second port connected to a sample filling portion for filling a sample, and an outlet port formed in the connecting portion for discharging components passing through the chromatographic column flow path to the detection unit; a switching component being switchable to: a first state connecting the second port and the sample holding flow path, and a second state connecting the first port, the sample holding flow path, the chromatographic column flow path, and the outlet port; the pump; the sample filling portion; and the detection unit.

[0013] The effects of the invention

[0014] According to the present invention, it is possible to improve the accuracy of sample analysis while also increasing convenience. Attached Figure Description

[0015] [ Figure 1 ] Figure 1 This is an exploded view illustrating the structure of a microfluidic device according to an embodiment of the present invention.

[0016] [ Figure 2 ] Figure 2 This is a plan view illustrating a connection example of a microfluidic device.

[0017] [ Figure 3 ] Figure 3 This is a plan view illustrating a connection example of a microfluidic device.

[0018] [ Figure 4 ] Figure 4 This is an exploded perspective view showing the state before the microfluidic device is installed on the separation column.

[0019] [ Figure 5 ] Figure 5 This is a cross-sectional view showing the state before the microfluidic device is installed on the separation column.

[0020] [ Figure 6 ] Figure 6 This is a cross-sectional view showing a microfluidic device mounted on a separation column.

[0021] [ Figure 7 ] Figure 7 This is an exploded perspective view of the microfluidic device involved in other embodiments.

[0022] [ Figure 8 ] Figure 8 This is an exploded perspective view of the microfluidic device involved in other embodiments.

[0023] [ Figure 9 ] Figure 9 This is an exploded perspective view of the microfluidic device involved in other embodiments.

[0024] [ Figure 10 ] Figure 10 This is a cross-sectional view of a microfluidic device involved in other embodiments.

[0025] [ Figure 11 ] Figure 11 This is a cross-sectional view showing a microfluidic device mounted on a separation column.

[0026] [ Figure 12 ] Figure 12 This is an exploded diagram illustrating the structure of the detector used in the fifth installation example.

[0027] [ Figure 13 ] Figure 13 This is a diagram illustrating the structure of a chromatography column mounting device.

[0028] [ Figure 14 ] Figure 14 This is a diagram illustrating the structure of a chromatography column mounting device.

[0029] [ Figure 15 ] Figure 15 This is a diagram illustrating the structure of a chromatography column mounting device.

[0030] [ Figure 16 ] Figure 16 This is an exploded view used to illustrate the structure of microfluidic devices involved in other embodiments.

[0031] [ Figure 17 ] Figure 17 This is a plan view illustrating a connection example of a microfluidic device.

[0032] [ Figure 18 ] Figure 18 This is a plan view illustrating a connection example of a microfluidic device. Detailed Implementation

[0033] Hereinafter, the microfluidic devices and connecting devices according to embodiments of the present invention will be described in detail with reference to the accompanying drawings.

[0034] (1) Structure of microfluidic devices

[0035] Figure 1This is an exploded view illustrating the structure of a microfluidic device according to an embodiment of the present invention. In the following description, the axis extending linearly in the vertical direction is referred to as the first axis AX1. Furthermore, as shown, the axis intersecting the first axis AX1 and extending linearly is referred to as the second axis AX2. One of the second axes AX2 is referred to as direction DA, and the other as direction DB. Furthermore, in the following description, arrow U represents the upward direction of the microfluidic device 1, and arrow D represents the downward direction of the microfluidic device 1.

[0036] The microfluidic device 1 includes a body portion 100 and a switching component 200. In this embodiment, the body portion 100 is composed of two plate-shaped components 110 and 120. The plate-shaped components 110 and 120 are formed of, for example, resin. The resin used in the plate-shaped components 110 and 120 is formed of, for example, polyetheretherketone (PEEK). The material of the plate-shaped components 110 and 120 can also be selected from metal, ceramic, glass, and resin. Furthermore, different materials can be selected for each of the plate-shaped components 110 and 120.

[0037] The plate-shaped component 110 is a flat plate having a top surface 110a and a bottom surface 110b. A connecting portion 130 is provided on the side of the plate-shaped component 110 in one direction DA. In this embodiment, the connecting portion 130 is a prismatic component protruding in one direction DA. The connecting portion 130 can be connected to a separation column (not shown) outside the microfluidic device 1. An example of the connection of the connecting portion 130 to the separation column will be described later. In this embodiment, the connecting portion 130 is integrally formed with the plate-shaped component 110. The plate-shaped component 110 includes multiple flow paths that are separated from each other. The multiple flow paths include flow paths 111-116, a sample holding portion 117, and a discharge flow path 118. Flow paths 111-116 are holes that pass through the top surface 110a and the bottom surface 110b. The sample holding portion 117 is a groove formed in the top surface 110a and connects flow paths 113 and 116. Figure 1 In this example, the sample holding portion 117 is formed in a straight line, but it can also be curved, for example. The shape of the sample holding portion 117 can be suitably determined, for example, according to the amount of sample that should be held. The discharge flow path 118 is a groove formed on the upper 110a and extends from the flow path 112 in one direction DA.

[0038] The connecting portion 130 includes a connecting flow path 131. The connecting flow path 131 is a groove extending in one direction DA and connected to the discharge flow path 118 of the plate-shaped member 110. A discharge port 132 is provided on the side of the connecting portion 130 in one direction DA. The connecting flow path 131 leads to the discharge port 132. The discharge port 132 opens the connecting flow path 131 to the outside. Figure 1In the example, the connection flow path 131 is formed by a corner column-shaped groove, but it can also be formed by a semi-circular groove.

[0039] The plate-shaped component 120 is a flat plate having a top surface 120a and a bottom surface 120b. The plate-shaped component 120 has a shape that overlaps with the plate-shaped component 110 and the connecting portion 130 when viewed from above. The plate-shaped component 120 functions as a cover for the top surface 110a of the plate-shaped component 110. When the connecting flow path 131 is formed by a semi-circular groove, a semi-circular groove can be formed in the bottom surface 120b at a position corresponding to the connecting flow path 131, making the discharge port 132 circular. When assembling the body portion 100, the plate-shaped component 110 and the plate-shaped component 120 are fixed together by bolts (not shown) with the bottom surface 120b abutting against the top surface 110a of the plate-shaped component 110. Alternatively, the plate-shaped component 110 and the plate-shaped component 120 can be integrated using joining or bonding techniques.

[0040] The plate-shaped component 120 includes flow paths 121, 124, and 125. Flow paths 121, 124, and 125 are holes that pass through the upper part 120a and the lower part 120b. Hereinafter, the portion of the upper part 120a in which the flow path 121 is formed is referred to as port A, the portion of the upper part 120a in which the flow path 124 is formed is referred to as port B, and the portion of the upper part 120a in which the flow path 125 is formed is referred to as port C. In top view, the flow path 121 of the plate-shaped component 120 overlaps with the flow path 111 of the plate-shaped component 110, the flow path 124 overlaps with the flow path 114, and the flow path 125 overlaps with the flow path 115.

[0041] In this embodiment, the switching member 200 is a rotor. The switching member 200 is formed of, for example, resin. The resin used in the switching member 200 is formed of, for example, polyetheretherketone (PEEK). The switching member 200 may also be formed of metal. The switching member 200 is a flat plate having a top surface 200a. Flow paths 210 to 230 are formed on the top surface 200a of the switching member 200. The flow paths 210 to 230 are arc-shaped grooves formed at different positions on the top surface 200a. Furthermore, the switching member 200 includes a motor and is capable of rotating about a first shaft AX.

[0042] When assembling the microfluidic device 1, the switching member 200 slidably contacts the lower surface 110b of the plate-shaped member 110 of the body portion 100. Thus, by rotating the switching member 200 relative to the body portion 100, multiple flow paths of the body portion 100 can be switched and selectively connected. In this embodiment, the switching member 200 can switch the state of the microfluidic device 1 to a first state and a second state. The states of the microfluidic device 1 will be described later.

[0043] Figure 2 as well as Figure 3 This is a plan view illustrating a connection example of the microfluidic device 1. Here, to explain the connection relationships of the flow paths within the body 100, the structural diagram of the microfluidic device 1 is simplified. (See diagram below.) Figure 2 as well as Figure 3 As shown, a pump 2 is connected to port A of the microfluidic device 1 via flow path P2. Pump 2 delivers the mobile phase contained in a container (not shown) to flow path P2. A sample filling section 3 is connected to port B via flow path P3. The sample filling section 3 is, for example, a needle that can be inserted into a container containing a sample. A syringe 4 is connected to port C via flow path P4. The syringe 4 can aspirate and expel liquid.

[0044] Figure 2 The microfluidic device 1 in its first state is shown. In the microfluidic device 1 in the first state, the flow path 220 of the switching component 200 connects port B and sample holding part 117, and the flow path 230 connects sample holding part 117 and port C. Thus, sample filling part 3 and syringe 4 are connected via sample holding part 117. Sample filling part 3 is, for example, a needle capable of piercing a container. With sample filling part 3 positioned inside a container containing a sample, the sample in the container is aspirated by syringe 4. Thus, the sample aspirated from sample filling part 3 can be held in sample holding part 117. Furthermore, if port C is provided with an injection port for inserting a needle and port B is connected to a drain port, the sample can also be aspirated with a needle and the needle inserted into the injection port, thereby filling the sample holding part 117 from port C.

[0045] Figure 3 The microfluidic device 1 in its second state is shown. In this second state, the sample holding section 117 is connected to the port A via flow path 230, and the sample holding section 117 is connected to the discharge flow path 118 via flow path 210. In this state, when the pump 2 is activated, the mobile phase is guided to the sample holding section 117. Furthermore, the sample and mobile phase held in the sample holding section 117 are discharged from the discharge port 132 via the discharge flow path 118. The mobile phase and sample discharged from the discharge port 132 are guided to a separation column connected to a connection section 130 (not shown).

[0046] According to this structure, since the microfluidic device filled with the sample is directly connected to the separation column, the piping connecting the sample injection section and the separation column is eliminated compared to conventional methods, thus shortening the flow path of the sample through to the separation column. This reduces the internal volume of the flow path from the sample holding section to the separation column, thereby suppressing sample diffusion within the mobile phase. Furthermore, since the connection between the microfluidic device 1 and the separation column and detector is an independent component, it is not necessary to replace the microfluidic device 1 itself according to analytical conditions; only the separation column or detector that does not meet the conditions needs to be replaced. Similarly, even if any component malfunctions, it is not necessary to replace the microfluidic device 1 itself. As a result, both the accuracy of sample analysis and convenience are improved.

[0047] (2) Installation of the separation column

[0048] (2-1) First installation example

[0049] Figure 4 This is an exploded perspective view showing the state before the microfluidic device 1 is installed on the separation column. Figure 5 This is a cross-sectional view showing the state before the microfluidic device 1 is installed on the separation column. Figure 6 This is a cross-sectional view showing the microfluidic device 1 mounted on the separation column. Here, the separation column connecting the microfluidic device 1 will be described first. (Refer to...) Figure 5 The separation column CL includes a port CZ. Port CZ includes a first recess Ca, a second recess Cb, and a third recess Cc. The first recess Ca includes a cylindrical inner circumferential surface Ca1 and a step portion Ca2. An internal thread is formed on the inner circumferential surface Ca1 of the first recess Ca. The second recess Cb includes a cylindrical inner circumferential surface Cb1, a step portion Cb2, and a frustum-shaped inner circumferential surface Cb3. The third recess Cc includes a cylindrical inner circumferential surface Cc1 and a bottom surface Cc2. A column flow path CP, connected to port CZ, is formed from the bottom surface Cc2 of the third recess Cc towards the DA side.

[0050] Reference Figure 4 as well as Figure 5 The microfluidic device 1 also includes a sleeve 300, a nut 400, and a clamp 500. The sleeve 300, nut 400, and clamp 500 are components used to connect the connecting portion 130 of the body portion 100 to the separation column CL.

[0051] The sleeve 300 is a cylindrical component extending along the second shaft AX2. The sleeve 300 is formed of a resin such as polyetheretherketone (PEEK). An insertion hole 301 extending along the second shaft AX2 and capable of mounting the connecting portion 130 is provided on the sleeve 300. In this embodiment, the length of the sleeve 300 is the same as the length of the connecting portion 130 along the second shaft AX2, but the sleeve 300 and the connecting portion 130 may also have different lengths. When connecting the microfluidic device 1 to the separation column CL, as... Figure 5 As shown, the connecting part 130 is fixed in the insertion hole 301 of the sleeve 300. An abutment surface 302 is formed on the end face of the sleeve 300 in the direction DA.

[0052] The nut 400 includes an external thread portion 410 and a tightening portion 420. The nut 400 is formed of a metal such as stainless steel (SUS). The nut 400 may also be formed of a resin such as polyetheretherketone (PEEK). The external thread portion 410 is a cylindrical component extending along the second shaft AX2 and has an external thread corresponding to the internal thread formed on the inner circumferential surface Ca1 of the first recess Ca of the separation column CL. The tightening portion 420 has a generally hexagonal prism shape. Through holes 401 are provided in both the external thread portion 410 and the tightening portion 420, respectively penetrating both portions. The through holes 401 have a diameter capable of inserting a sleeve 300. When connecting the microfluidic device 1 to the separation column CL, as... Figure 5 As shown, the sleeve 300 is inserted into the through hole 401.

[0053] The ferrule 500 includes a tapered portion 510 and an annular component 530. The ferrule 500 is formed of a metal such as stainless steel (SUS). The ferrule 500 may also be formed of a resin such as polyetheretherketone (PEEK). The tapered portion 510 is a frustum-shaped component and has an outer circumferential surface Cb3 corresponding to the inner circumferential surface Cb1 of the second recess Cb of the separation column CL. The annular component 530 is a cylindrical component and has an outer circumferential surface Cb1 corresponding to the inner circumferential surface Cb1 of the second recess Cb of the separation column CL. Through holes 501 are provided in the tapered portion 510 and the annular component 530, respectively penetrating the tapered portion 510 and the annular component 530. The through holes 501 have a diameter capable of housing the sleeve 300. When connecting the microfluidic device 1 to the separation column CL, as... Figure 5 As shown, the sleeve 300 is fixed in the through hole 501.

[0054] With the external thread 410 engaged with the internal thread formed on the inner circumferential surface Ca1 of the first recess Ca, the nut 400 is advanced in one direction DA by tightening the tightening part 420 with a tool such as a wrench. As a result, the ferrule 500 is pressed in one direction DA. In this situation, since the sleeve 300 is fixed in the through hole 501 of the ferrule 500 and the connecting part 130 is fixed in the insertion hole 301 of the sleeve 300, the sleeve 300 and the connecting part 130 are pressed in one direction DA by the nut 400. As a result, the abutment surface 302 is pressed against the bottom surface Cc2 of the third recess Cc. In this state, when the tightening part 420 is further tightened, as... Figure 6 As shown, the connecting flow path 131 of the connecting part 130 can be connected to the column flow path CP of the separation column CL.

[0055] (2-2) Second installation example

[0056] In the above embodiment, the connecting part 130 is a corner-shaped component that protrudes in one direction DA, but the present invention is not limited thereto. Figure 7 This is an exploded perspective view of the microfluidic device 1A according to other embodiments. Figures 1-6 Microfluidic devices 1 and Figure 7 The microfluidic device 1A differs in the following aspects. The connecting portion 130A of the microfluidic device 1A is a cylindrical component protruding in one direction DA. The diameter r of the end face of the connecting portion 130A with the discharge port 132 is set to a size that allows it to be inserted into the through hole 401 of the nut 400 and mounted in the ferrule 500. In this case, since there is no need to provide a sleeve 300 for mounting the connecting portion 130A to the ferrule 500, the number of components used to connect the separation column CL can be reduced.

[0057] (2-3) Third installation example

[0058] exist Figure 7 In the microfluidic device 1A, the connecting portion 130A is formed from a single cylindrical component, but the present invention is not limited thereto. Figure 8 as well as Figure 9 This is an exploded perspective view of the microfluidic device 1B according to other embodiments. Figure 7 Microfluidic device 1A and Figure 8 as well as Figure 9The microfluidic device 1B differs in the following aspects. The microfluidic device 1B also includes an end-face sealing component 600. The end-face sealing component 600 is a cylindrical component having an abutment surface 602 and a pressing surface 604, and a through-hole 601 penetrating the abutment surface 602 and the pressing surface 604. The end-face sealing component 600 is formed of resin. The through-hole 601 is a hole for connecting to the column flow path CP of the separation column CL. The abutment surface 602 of the end-face sealing component 600 corresponds to the bottom surface Cc2 of the third recess Cc of the separation column CL. The outer peripheral surface 603 of the end-face sealing component 600 corresponds to the inner peripheral surface Cc1 of the third recess Cc of the separation column CL.

[0059] The microfluidic device 1B includes a connector 130B and a nut 400B. For example... Figure 8 As shown, the connecting portion 130B includes a first component 133 and a second component 134 extending along the second axis AX2. The first component 133 is a cylindrical shape with a diameter r1. The second component 134 is a cylindrical shape with a diameter r2 larger than the diameter r1. Therefore, a step portion 134a is formed on the end face of the second component 134 in the other direction DB side. Furthermore, an end face 134b including a discharge port 132 is formed on the end face of the second component 134 in one direction DA side. In the first component 133 and the second component 134, a step portion 134a is formed with the second component 130B. Figures 1-6 Similarly, the connecting part 130 is formed with a connecting flow path 131.

[0060] like Figure 9 As shown, the nut 400B has a slit SL extending along the second axis AX2 from its end face in the other direction DB to its end face in the other direction DA. The width W1 of the slit SL is set to be greater than the diameter r1 of the first component 133 and less than the diameter r2 of the second component 134. The diameter r3 of the through hole 401 of the nut 400B is set to be greater than the diameter r1 of the first component 133 and less than the diameter r2 of the second component 134. In the nut 400B, the first component 133 of the connecting part 130B is fitted into the through hole 401 through the slit SL. When connecting the microfluidic device 1B to the separation column CL, the tightening part 420 is tightened with a tool such as a wrench while the end face 411 of the nut 400B in the other direction DA is in contact with the step portion 134a of the second component 134. In this configuration, the pressing surface 604 of the end-face sealing member 600 is pressed against the end face 134b of the second member 134 of the connecting portion 130B. This allows the microfluidic device 1B to be connected to the separation column CL. According to this microfluidic device 1B, since it can be installed into the separation column CL without the need for a ferrule 500, the sample and mobile phase flowing in the flow path will not wrap around the ferrule, thereby suppressing sample diffusion and carry-over.

[0061] (2-4) Fourth installation example

[0062] In the above embodiments, an example is described of using a nut or a nut and a ferrule to connect the main body 100 to the separation column CL, but the present invention is not limited thereto. Figure 10 This is a cross-sectional view of the microfluidic device 1C according to other embodiments. Figure 11 This is a cross-sectional view showing the microfluidic device 1C mounted on the separation column. Figures 1-6 Microfluidic device 1 differs from microfluidic device 1C in the following aspects. Microfluidic device 1C also has a column support mechanism 700 to replace the nut or nut and ferrule.

[0063] The column support mechanism 700 includes a receiving component 710, a spring 720, and a fixing component 730. The receiving component 710 includes a covering component 711 and a plate component 712. The plate component 712 has a through hole 712a through which the sleeve 300 passes. Inside the covering component 711 and the plate component 712, a space SP is formed to accommodate a protrusion PA protruding outward around the port CZ of the separation column CL. An insertion hole 711a is formed on one end face of the covering component 711 facing DA. A engaging portion 713 protruding inward toward the second axis AX2 can engage with the step portion PB of the protrusion PA. The receiving component 710 is provided with a cam clamp 714, which can adjust the position of the engaging portion 713 relative to the plate component 712 to an open state and a closed state. When the cam clamp 714 is in the open state, the size of the insertion hole 711a is such that the protrusion PA of the separation column CL can be inserted into the space SP. When the cam clamp 714 is in the closed state, the size of the insertion hole 711a becomes the size of the protrusion PA that cannot be inserted into the space SP into the space SP.

[0064] The fixing member 730 is a cylindrical component fixed around the second shaft AX2 of the sleeve 300, and has an abutment surface 731 that can abut against the end face PC on the other direction DB side of the separating column CL. One end of a spring 720 is connected to the end face 732 on the other direction DB side of the fixing member 730. The other end of the spring 720 is connected to the plate member 712. The spring 720 is a compression spring, and when the spring 720 contracts, a force acts in the direction in which the spring 720 extends. Therefore, when a force in the other direction DB is applied to the fixing member 730, a force is generated by the spring 720 that presses the fixing member 730 in one direction DA.

[0065] When installing the microfluidic device 1C, with the cam clamp 714 in the open state, the protrusion PA of the separating column CL is inserted into the space SP. In this state, the retaining member 730 is pressed against the protrusion PA of the separating column CL in the opposite direction DB. This causes the spring 720 to contract. In this state, the cam clamp 714 is switched to the closed state. In this case, as... Figure 11 As shown, the fixing member 730 is pressed in one direction DA by the spring 720, so that the protrusion PA of the separation column CL is pressed onto the engaging part 713. Thus, since the separation column CL is fixed in the space SP of the column support mechanism 700, the connecting part 130 and the separation column CL can be connected.

[0066] (2-5) Fifth installation example

[0067] In the fifth installation example, a column mounting device capable of connecting the microfluidic device 1, the separation column, and the detector was used. First, the detector will be described. The detector used here is a chip with general photodetector functionality. Figure 12 This is an exploded diagram used to illustrate the structure of the detector used in this example.

[0068] Detector Z includes plate-shaped components 100Z and 200Z. Plate-shaped component 100Z includes through holes ZA, ZB, ZC, and ZD. Through hole ZA is connected to one end of a first flow path hole (not shown) formed within plate-shaped component 100Z, the other end of which is connected to through hole ZB. Thus, through hole ZA is connected to through hole ZB. Through hole ZC is connected to one end of a second flow path hole (not shown) formed within plate-shaped component 100Z, the other end of which is connected to through hole ZD. Thus, through hole ZC is connected to through hole ZD.

[0069] Flow paths Z1, Z2, and Z3, separated from each other, are formed on the plate-shaped component 100Z. Flow path Z1 is a groove extending along the second axis AX2 between a port ZZ, which opens to the side of the plate-shaped component 100Z in one direction (DA), and a through hole ZA. Flow path Z2 is a groove extending along the third axis AX3, intersecting the second axis AX2, between through holes ZB and ZC. Flow path Z3 is a groove extending along the second axis AX2 between a through hole ZD and a port ZY, which opens to the side of the plate-shaped component 100Z in another direction (DB). In this example, port ZZ is connected to the separation column CL, and port ZY is connected to a drainage mechanism (not shown). When analyzing a sample, the mobile phase and sample guided from the separation column CL are introduced into the detector Z through port ZZ. The mobile phase and sample are sequentially guided through flow paths Z1, Z2, and Z3, and then guided from port ZY to the drainage mechanism (not shown). Flow path Z2 of the detector Z functions as a flow cell. The two ends of flow path Z2 are formed of light-transmitting components, such as glass.

[0070] Outside the plate-shaped component 100Z, a light source unit 10Z, an absorbance detection unit 20Z, and a fluorescence detection unit 30Z are disposed. The light source unit 10Z and the absorbance detection unit 20Z are positioned facing each other, sandwiching the flow path Z2 on the third axis AX3. The fluorescence detection unit 30Z is disposed between the light source unit 10Z and the absorbance detection unit 20Z. The light source unit 10Z includes a light source such as a deuterium lamp and a diffraction grating capable of dispersing the light from the light source. The absorbance detection unit 20Z is, for example, an ultraviolet-visible absorbance detector (UV-VIS), which uses the light emitted from the light source unit 10Z to measure the change in absorbance of the sample passing through the flow path Z2. The fluorescence detection unit 30Z includes, for example, a photomultiplier tube, which converts the light emitted by the fluorescent material contained in the sample within the flow path Z2 into an electrical signal. During the assembly of detector Z, plate-shaped component 100Z and plate-shaped component 200Z are fixed together by bolts (not shown) or the like, with plate-shaped component 100Z abutting against each other. Alternatively, plate-shaped component 110Z and plate-shaped component 120Z can be integrated by joining or bonding techniques.

[0071] Figures 13-15 This is a diagram illustrating the structure of the chromatographic column mounting device 800. (For example...) Figure 13 As shown, the microfluidic device 1 used in this example does not have a protruding connection portion 130 (see reference). Figure 1 A portion of the side surface of the main body 100 is configured as a connecting portion 130E, which has a perforated discharge port 132. For example... Figure 14 As shown, the column mounting apparatus 800 includes a body alignment device 810, a detector chip alignment device 820, a guide rail 830, a clamping mechanism 840, a first sealing adapter 850, and a second sealing adapter 860. The body alignment device 810 includes a mounting portion 811, an outer frame 812, and a partition wall portion 813. The mounting portion 811 is a flat plate-shaped component with a top surface 811a. A downwardly protruding protrusion 811b is provided on the mounting portion 811. The outer frame 812 and the partition wall portion 813 are provided on the top surface 811a of the mounting portion 811. The outer frame 812 and the partition wall portion 813 are disposed on the top surface 811a to internally accommodate the microfluidic device 1. A through hole 813a extending along the fourth axis AX4 is provided in the partition wall portion 813.

[0072] The detector chip aligner 820 includes a mounting portion 821, an outer frame 822, and a partition portion 823. The mounting portion 821 is a flat plate-shaped component having a top surface 821a. The mounting portion 821 has a downwardly protruding protrusion (not shown, but with the same structure as protrusion 811b). The outer frame 822 and the partition portion 823 are provided on the top surface 821a of the mounting portion 821. The outer frame 822 and the partition portion 823 are disposed on the top surface 821a to internally accommodate the detector Z. A through hole 823a extending along the fourth axis AX4 is provided in the partition portion 823.

[0073] The body alignment device 810 and the detector chip alignment device 820 are movably disposed on the guide rail 830. Specifically, at least one of the body alignment device 810 and the detector chip alignment device 820 is configured to be movable relative to the other. In this embodiment, the detector chip alignment device 820 is fixed on the guide rail 830, and the body alignment device 810 is configured to be movable on the guide rail 830 relative to the fixed detector chip alignment device 820.

[0074] The body aligner 810 and the detector chip aligner 820 are connected via a clamping mechanism 840. Specifically, the protrusion 811b of the body aligner 810 and the protrusion (not shown) of the detector chip aligner 820 are connected by the clamping mechanism 840. The clamping mechanism 840 is, for example, a tension spring. Therefore, for example, when the body aligner 810 is moved away from the detector chip aligner 820, a force is applied that causes the body aligner 810 to move toward the detector chip aligner 820.

[0075] like Figure 15 As shown, the first sealing adapter 850 includes a sleeve portion 851, a shaft component 852, and an end face sealing component 853. Regarding the sleeve portion 851, it has a... Figures 4-6 The sleeve 500 has the same structure. The shaft member 852 is a rod-shaped member extending along the fourth axis AX4. Through holes 850a are formed in both the sleeve portion 851 and the shaft member 852, respectively penetrating through both. The end face sealing member 853 is a flat plate-shaped sealing member extending along the fourth axis AX4. A through hole 850b is formed in the end face sealing member 853, penetrating along the fourth axis AX4. The shaft member 852 and the end face sealing member 853 can be inserted into the through hole 813a of the partition wall portion 813.

[0076] The second sealing adapter 860 includes a sleeve portion 861, a shaft component 862, and an end face sealing component 863. Regarding the sleeve portion 861, it has a... Figures 4-6The sleeve 500 has the same structure. The shaft member 862 is a rod-shaped member extending along the fourth axis AX4. Through holes 860a are formed in both the sleeve portion 861 and the shaft member 862, respectively penetrating both. The end-face sealing member 863 is a flat plate-shaped sealing member extending along the fourth axis AX4. A through hole 860b is formed in the end-face sealing member 863, penetrating along the fourth axis AX4. The shaft member 862 and the end-face sealing member 863 can be inserted into the through hole 823a of the partition wall portion 823.

[0077] Reference Figures 13-15 In the assembly of the column mounting apparatus 800, firstly, the microfluidic device 1 is fixed inside the outer frame 812 of the upper part 811a of the mounting portion 811. At this time, the microfluidic device 1 can also be further fixed to the upper part 811a of the mounting portion 811 by bolts or the like. When the microfluidic device 1 is fixed inside the outer frame 812, the center of the discharge port 132 of the microfluidic device 1, the center of the through hole 813a of the partition wall portion 813, and the fourth axis AX4 are aligned. The detector Z is fixed inside the outer frame 822 of the upper part 821a of the mounting portion 821. At this time, the detector Z can also be further fixed to the upper part 821a of the mounting portion 821 by bolts or the like. When the detector Z is fixed inside the outer frame 822, the center of the port ZY of the detector Z, the center of the through hole 823a of the partition wall portion 823, and the fourth axis AX4 are aligned.

[0078] Next, with the body alignment 810 moved away from the detector chip alignment 820, when the end face sealing member 853 and the shaft member 852 are inserted into the through hole 813a, the first sealing adapter 850 is fixed to the body alignment 810. Furthermore, when the end face sealing member 863 and the shaft member 862 are inserted into the through hole 823a, the second sealing adapter 860 is fixed to the detector chip alignment 820. In this state, the sleeve portion 851 of the first sealing adapter 850 and the sleeve portion 861 of the second sealing adapter 860 are respectively inserted into the ports CZ at both ends of the separation column CL.

[0079] In this state, the body alignment device 810 is brought close to the detector chip alignment device 820. The clamping force of the clamping mechanism 840 allows the separation column CL to be clamped between the body alignment device 810 and the detector chip alignment device 820. Thus, the microfluidic device 1, the separation column CL, and the detector Z can be connected using only one column support mechanism 700.

[0080] (3) Other implementation methods

[0081] In the above embodiments, an example is described where the main body 100 is composed of two plate-shaped members 110 and 120; however, the present invention is not limited thereto. The main body 100 may be composed of one plate-shaped member or two or more plate-shaped members. Figure 16 This is an exploded view used to illustrate the structure of the microfluidic device 1D involved in other embodiments.

[0082] exist Figure 16 In the microfluidic device 1D, the body portion 100D includes plate-shaped components 140, 150, 160, 170, 180, and 190. The plate-shaped components 140, 150, 160, 170, 180, and 190 are formed of, for example, resin. The resin used in the plate-shaped components 110 and 120 is formed of, for example, polyetheretherketone (PEEK). The material of the plate-shaped components 140, 150, 160, 170, 180, and 190 can also be selected from metal, ceramic, glass, and resin.

[0083] The plate-shaped component 150 is a flat plate having a top surface 150a and a bottom surface 150b. The plate-shaped component 150 includes a plurality of flow paths separated from each other. The plurality of flow paths include flow paths 151, 152, 154, and 159. Flow paths 151, 152, 154, and 159 are holes that pass through the top surface 150a and the bottom surface 150b.

[0084] The plate-shaped component 160 is a flat plate having a top surface 160a and a bottom surface 160b. The plate-shaped component 160 includes multiple flow paths separated from each other. These flow paths include flow paths 161, 162, 164, 165, 169 and a column flow path CP. Flow paths 161, 162, 164, 165, and 169 are holes penetrating the top surface 160a and the bottom surface 160b. The column flow path CP is a groove formed in the top surface 160a and connects flow paths 165 and 169. The column flow path CP has a tortuous shape, but it can also be straight. The shape of the column flow path CP can also be suitably determined according to the analytical conditions. A top cover (not shown) is provided on the top of the column flow path CP, and the column flow path CP is filled with general packing material used for separation columns.

[0085] The plate-shaped component 170 is a flat plate having a top surface 170a and a bottom surface 170b. The plate-shaped component 170 includes a plurality of flow paths separated from each other. The plurality of flow paths include flow paths 171, 172, 174, and 175. Flow paths 171, 172, 174, and 175 are holes that pass through the top surface 170a and the bottom surface 170b.

[0086] The plate-shaped component 180 is a flat plate having a top surface 180a and a bottom surface 180b. The plate-shaped component 180 includes multiple flow paths separated from each other. The multiple flow paths include flow paths 181, 182, 183, 184, 185, 186 and a sample holding section SH. Flow paths 181, 182, 183, 184, 185, 186 are holes penetrating the top surface 180a and the bottom surface 180b. The sample holding section SH is a groove formed in the top surface 180a and connects flow paths 183 and 186. The sample holding section SH and... Figure 1 The sample holding section 117 of the microfluidic device 1 has the same function. The sample holding section SH has a tortuous shape, but it can also be straight. The shape of the sample holding section SH can also be appropriately determined according to the amount of sample to be held.

[0087] The plate-shaped component 140 is a flat plate having a top surface 140a and a bottom surface 140b. A connecting portion 130D protruding along a second axis AX2 in one direction DA is provided on the side of the plate-shaped component 140. A connecting flow path 131 extending along the second axis AX2 is formed in the connecting portion 130D. The connecting flow path 131 may also include an atomizing flow path 131D. A gas supply portion GS capable of supplying atomizing gas into the atomizing flow path 131D is connected to the connecting portion 130D. Furthermore, a voltage application portion 133D surrounding the atomizing flow path 131D is provided on the connecting portion 130D. The voltage application portion 133D is connected to a power supply device (not shown). The voltage application portion 133D applies a high voltage of approximately 3 to 5 kV to the sample flowing through the atomizing flow path 131D. Through these structures, electrospray ionization (ESI) can be implemented in the connector 130D. As a result, a mist-like sample can be ejected from the ejection port 132 of the connector 130D. The connector 130D is connected to an external detector (not shown). The detector is, for example, a quadrupole mass detector.

[0088] The plate-shaped component 140 includes multiple flow paths separated from each other. These flow paths include flow paths 141, 142, 144, 149 and a discharge flow path 118D. Flow paths 141, 142, 144, and 149 are holes passing through the upper part 140a and the lower part 140b. The structure and function of the discharge flow path 118D are described in detail below. Figure 1 The discharge path 118 of the microfluidic device 1 is the same.

[0089] Plate-shaped member 190 is a flat plate having a top surface 190a and a bottom surface 190b. Plate-shaped member 190 has a shape that overlaps with plate-shaped member 140 and connecting portion 130D when viewed from above. Plate-shaped member 190 functions as a cover for the top surface 140a of plate-shaped member 140. Plate-shaped member 190 includes flow paths 191, 192, and 194. Flow paths 191, 192, and 194 are holes that pass through the top surface 190a and the bottom surface 190b. Hereinafter, the portion of the top surface 120a in which flow path 121 is formed will be referred to as port AA, the portion of the top surface 120a in which flow path 124 is formed will be referred to as port BB, and the portion of the top surface 120a in which flow path 125 is formed will be referred to as port CC.

[0090] Regarding the switching component 200, and Figure 1 The switching component 200 is the same. When assembling the main body 100D, the plate-shaped components 140, 150, 160, 170, 180, and 190 are overlapped vertically, and each is fixed by bolts (not shown). At this time, in top view, flow paths 191, 141, 151, 161, 171, and 181 overlap; flow paths 192, 142, 152, 162, 172, and 182 overlap; flow paths 194, 144, 154, 164, 174, and 184 overlap; and flow paths 165, 175, and 185 overlap. Therefore, when assembling the main body 100D, flow paths 191, 141, 151, 161, 171, and 181 are each connected, and flow paths 192, 142, 152, 162, 172, and 182 are each connected. Furthermore, flow paths 194, 144, 154, 164, 174, and 184 are each connected, and flow paths 165, 175, and 185 are each connected.

[0091] During the assembly of the microfluidic device 1D, the switching member 200 slidably contacts the lower surface 180b of the plate-shaped member 180 of the body portion 100D. Thus, by rotating the switching member 200 relative to the body portion 100D, the switching member 200 can switch multiple flow paths of the body portion 100 and selectively connect them.

[0092] Figure 17 as well as Figure 18 This is a plan view illustrating a connection example of the microfluidic device 1D. Here, to illustrate the connection relationships of the flow paths, the structural diagram of the microfluidic device 1 is simplified, and the plan views of each plate-shaped component are shown side-by-side. (See diagram below.) Figure 17 as well as Figure 18As shown, in port AA, a sample filling section 3 is connected via flow path P11. The sample filling section 3 is, for example, a needle that can be inserted into a container containing a sample. In port BB, a syringe 4 is connected via flow path P12. The syringe 4 can aspirate and expel liquid. In port CC, a pump 2 is connected via flow path P13. The pump 2 delivers the mobile phase contained in a container (not shown) to flow path P2.

[0093] Figure 17 The diagram shows a microfluidic device 1D in its first state. In the microfluidic device 1D in its first state, flow paths 182 and 183 are connected via flow path 210 of switching component 200, flow paths 184 and 185 are connected via flow path 220 of switching component 200, and flow paths 186 and 181 are connected via flow path 230 of switching component 200. Thus, syringe 4 is connected to sample filling section 3 via flow paths 192, 142, 152, 162, 172, 182, flow paths 210 and 183 of switching component 200, sample holding section SH, flow path 186, flow path 230 of switching component 200, and flow paths 181, 171, 161, 151, 141, 191. Therefore, with sample filling section 3 positioned within a container holding the sample, the sample is aspirated from the container by syringe 4. Thus, the sample drawn from the sample filling section 3 is held in the sample holding section SH.

[0094] Figure 18 The microfluidic device 1D in its second state is shown. In the microfluidic device 1D in its second state, flow paths 181 and 182 are connected via flow path 210 of switching component 200, flow paths 183 and 184 are connected via flow path 220 of switching component 200, and flow paths 185 and 186 are connected via flow path 230 of switching component 200. In this case, flow paths 194, 144, 154, 164, 174, 184, flow paths 220 and 183 of switching component 200, sample holding section SH, flow path 186, flow paths 230, 185, 175, 165 of switching component 200, column flow paths CP, flow paths 169, 159, 149, and connection flow path 131 of connection section 130D are connected. Therefore, pump 2 is connected to discharge port 132 via sample holding section SH and column flow path CP. Thus, in the first state, the sample held in the sample holding section SH can be guided to the column flow path CP. Furthermore, the sample separated by substance through the column flow path CP is guided to the nebulization flow path 131D of the connecting flow path 131, and the atomized sample is guided to the detector connected to the outside of the connecting section 130D.

[0095] According to this structure, since flow paths and separation columns are formed on multiple plate-shaped components (chips), the flow path to the separation column is shorter compared to cases where flow paths are formed by piping or the like. This reduces the internal volume of the flow path through which the mobile phase flows to the separation column, thus suppressing sample diffusion within the mobile phase. Furthermore, even if the separation capability of the chromatographic column flow path does not meet the analytical requirements, a microfluidic device suitable for the analytical conditions can be obtained by replacing only the plate-shaped component 160 instead of all the microfluidic devices. As a result, both analytical accuracy and convenience are improved. Moreover, by replacing the plate-shaped components 180 or 140 according to the capacity of the sample holding section or the connection shape of the detector used, a microfluidic device suitable for the analytical conditions can be easily obtained.

[0096] (4) The correspondence between the constituent elements of the claims and the parts of the embodiments.

[0097] Hereinafter, examples of the correspondence between the constituent elements of the claims and the elements of the embodiments will be described. In the above embodiments, the connecting flow path 131 is an example of an inlet flow path, port A and port CC are examples of first ports, port B and port AA are examples of second ports, the sample holding part SH is an example of a sample ring, and the spring 720 and the fixing member 730 are examples of pressing parts. Furthermore, the body alignment member 810 is an example of a first fixing member, and the detector chip alignment member 820 is an example of a second fixing member. In addition, the plate-shaped member 140 is an example of a first plate-shaped member, the plate-shaped member 160 is an example of a second plate-shaped member, and the plate-shaped member 180 is an example of a third plate-shaped member.

[0098] (5) Scheme

[0099] Those skilled in the art will understand that the above-described exemplary embodiments are specific examples of the following scheme.

[0100] (Item 1) relates to a microfluidic device of one embodiment.

[0101] It has a body section consisting of one or more components.

[0102] The body portion includes:

[0103] The connecting part can be connected to the separation column; and

[0104] Multiple flow paths

[0105] The multiple flow paths include:

[0106] Sample holding section, for holding the sample; and

[0107] The inlet flow path enables the sample to be introduced into the separation column for sample separation.

[0108] The microfluidic device further includes: a switching component slidably connected to the body portion, and capable of selectively connecting the plurality of flow paths by sliding relative to the body portion.

[0109] According to the microfluidic device described in item 1, since the microfluidic device filled with the sample is directly connected to the separation column, there is no need for piping connecting the sample injection section and the separation column compared to the past, thus shortening the flow path of the sample through to the separation column. This reduces the internal volume of the flow path from the sample holding section to the separation column, thereby suppressing sample diffusion within the mobile phase. Furthermore, since the microfluidic device and the separation column are separate components, only the separation column needs to be replaced if it is desired for other analyses. As a result, both the accuracy of sample analysis and convenience are improved.

[0110] (Item 2) In the microfluidic devices described in Item 1,

[0111] The connecting part is a prism shape extending in one direction.

[0112] The microfluidic device may further include:

[0113] The sleeve is penetrated by the connecting part, has an inner circumferential surface that can engage with the connecting part, and has a cylindrical outer circumferential surface.

[0114] The nut has a first through hole through which the sleeve passes and a first threaded groove for engaging with a main threaded groove formed on the inlet port of the separating column; and

[0115] The ferrule has a second through hole through which the sleeve passes and can seal the flow path within the separation column.

[0116] According to the microfluidic device described in item 2, the connecting part is inserted into the sleeve, thereby engaging the connecting part with the sleeve. Furthermore, with the sleeve passing through the nut and the ferrule, the main thread groove of the separating column is screwed into the first thread groove of the nut. In this case, the ferrule and the connecting part are pushed towards the separating column. Thus, the flow path within the separating column is connected to the flow path within the main body, while the separating column is sealed. Therefore, the microfluidic device can be connected to the separating column with a simple structure.

[0117] (Item 3) In the microfluidic devices described in Item 1,

[0118] The connecting part is a cylindrical shape extending in one direction.

[0119] The microfluidic device may further include:

[0120] The nut has a first through hole through which the connecting portion passes and a first threaded groove for engaging with a main threaded groove formed on the inlet port of the separating column; and

[0121] The sleeve has a second through hole through which the connecting part passes and can seal the flow path within the separating column.

[0122] According to the microfluidic device described in item 3, with the connecting part passing through the nut and the ferrule, the main thread groove of the separating column is screwed into the first thread groove of the nut. In this case, the ferrule and the connecting part are pushed towards the separating column. Thus, the flow path within the separating column is connected to the flow path within the main body. With this structure, a sleeve for connecting the connecting part and the separating column is not required. Therefore, the microfluidic device can be connected to the separating column with a simpler structure.

[0123] (Item 4) The microfluidic device described in Item 1 may further include:

[0124] The nut has a first threaded groove capable of engaging with a main threaded groove formed on the inlet port of the separating column.

[0125] The connecting portion may also include:

[0126] The first cylindrical component extends in one direction; and

[0127] The second cylindrical component extends further from the first cylindrical component in the direction and has a larger diameter than the first cylindrical component, and has a sealing surface intersecting the direction.

[0128] The nut may also contain:

[0129] The first through hole allows the first cylindrical component to pass through; and

[0130] The gap extends in the said direction and allows the first cylindrical component to be installed into the first through hole.

[0131] According to the microfluidic device described in item 4, with the first cylindrical component positioned in the first through-hole through the gap, the second cylindrical component is pressed in one direction by the engagement of the main thread groove of the separator and the first thread groove of the nut. This pushes the sealing surface against the separator. Thus, while connecting the flow path within the separator and the second flow path, the separator is sealed. Therefore, it is possible to connect to the separator with a simple structure.

[0132] (Item 5) The microfluidic device described in Item 1,

[0133] It can also be further equipped with a column support mechanism.

[0134] The connecting part is a column shape extending in one direction.

[0135] The separating column has a port for connecting to the connecting portion, and a protrusion protruding outward around the port.

[0136] The column support mechanism may also include:

[0137] A receiving component has an insertion hole for inserting the protrusion of the separating post, and a receiving space for receiving the protrusion; and

[0138] The pressing part is fixed to the connecting part within the receiving space and is configured to press the protrusion onto the receiving member so that the receiving member engages with the protrusion.

[0139] According to the microfluidic device described in item 5, the body can be connected to the separation column without the need for ferrules, nuts, or similar structures. Furthermore, since tightening the nut is unnecessary when connecting to the separation column, no tools are required. Therefore, connecting the body to the separation column can be made much easier.

[0140] (Item 6) In any of the microfluidic devices described in items 1 through 5,

[0141] The multiple components can also be plate-shaped.

[0142] According to the microfluidic device described in item 6, it is possible to achieve thinning.

[0143] (Item 7) In any of the microfluidic devices described in items 1 through 6,

[0144] The switching component can also be switched to:

[0145] The state of connection between the sample holding part and the inlet flow path; and

[0146] The sample holding section and the inlet flow path are not connected.

[0147] In the microfluidic device described in item 7, the connection state between the sample holding section and the inlet flow path can be easily switched.

[0148] (Item 8) In liquid chromatographs involving other schemes, the following features are provided:

[0149] The microfluidic device described in any one of items 1 to 6, wherein,

[0150] The plurality of flow paths further include:

[0151] The first port is connected to the pump that delivers the mobile phase; and

[0152] The second port is connected to the sample filling section for filling the sample.

[0153] The switching component can be switched to:

[0154] The first state of connecting the second port and the sample holding part; and

[0155] The second state connecting the first port, the sample holding part, and the inlet flow path;

[0156] The pump;

[0157] The sample filling section;

[0158] The separation column; and

[0159] The detection department detects the components that have passed through the separation column.

[0160] According to the liquid chromatograph described in item 8, when the switching unit is in the first state, the sample aspirated by the sample packing section is guided from the second port to the sample loop. Thus, the sample is held within the sample loop. When the switching unit is in the second state, the mobile phase pumped out is guided from the first port to the sample loop. Thus, the sample held within the sample loop, together with the mobile phase, is guided to the separation column. In this way, until the mobile phase is guided to the separation column, since there are no unnecessary flow paths such as piping, the diffusion of the sample within the mobile phase can be suppressed.

[0161] (Item 9) A connection device relating to other solutions, which is a connection device for connecting the microfluidic device of claim 1 or 2, the separation column, and the detector, comprising:

[0162] The first fixing component is capable of fixing the microfluidic device and connecting the connecting portion of the microfluidic device to the inlet port of the separation column;

[0163] The second fixing component is capable of fixing the detector and can be connected to the inlet port of the detector and the outlet port of the separation column;

[0164] The guide rail is configured to allow at least one of the first fixing member and the second fixing member to move relative to the other; and

[0165] The clamping mechanism is capable of reducing the distance between the first fixing component and the second fixing component disposed on the guide rail.

[0166] According to the connecting device described in item 9, the separation column is positioned between the first and second fixed components while the distance between the first and second fixed components is increased. In this case, the separation column can be clamped between the first and second fixed components using a clamping mechanism, and the microfluidic device, the separation column, and the detector can be connected. Therefore, the microfluidic device, the separation column, and the detector can be connected with a simpler structure.

[0167] (Item 10) Microfluidic devices involving other solutions, possessing:

[0168] The main body has multiple flow paths; and

[0169] A switching component is slidably connected to the main body and can selectively connect the multiple flow paths of the main body by sliding relative to the main body.

[0170] The body portion includes:

[0171] First plate-shaped component; and

[0172] Second plate-shaped component,

[0173] The multiple flow paths include:

[0174] A sample retention flow path is formed in the first plate-shaped component and retains the sample; and

[0175] The chromatographic column flow path is formed in the second plate-shaped component and is capable of separating the sample by components.

[0176] According to the microfluidic device described in item 10, since flow paths and column flow paths are formed on multiple plate-shaped components (chips), the flow path up to the sample separation component is shorter compared to cases where flow paths are formed by piping or the like. This reduces the internal capacity of the flow path through which the mobile phase flows, thus suppressing sample diffusion within the mobile phase. Furthermore, the multiple plate-shaped components consist of a first plate-shaped component with a sample holding flow path and a second plate-shaped component with a column flow path. In this case, when changing the capacity of the sample holding flow path, only the first plate-shaped component containing the sample holding flow path needs to be replaced; when the column flow path does not meet the analytical requirements, only the second plate-shaped component containing the column flow path needs to be replaced. As a result, when changing the structure of the microfluidic device according to analytical conditions, only a portion of its structure needs to be replaced, thereby improving both the accuracy and convenience of sample analysis.

[0177] (Item 11) In the microfluidic devices described in Item 10,

[0178] The body portion may further include:

[0179] The third plate-shaped component,

[0180] The third plate-shaped component includes a connecting portion that can be connected to a detection portion for detecting components that have passed through the chromatographic column flow path.

[0181] The multiple flow paths include:

[0182] The first port is connected to the pump that delivers the mobile phase;

[0183] The second port is connected to the sample filling section filled with the sample; and

[0184] An outlet port, formed in the connecting portion, discharges components that have passed through the chromatographic column flow path to the detection portion.

[0185] The switching component can be switched to:

[0186] The second port and the sample maintain the first state of the flow path; and

[0187] The second state connecting the first port, the sample holding flow path, the chromatographic column flow path, and the ejection port.

[0188] According to the microfluidic device described in item 11, when the switching unit is in the first state, the sample aspirated by the sample suction unit is guided from the second port to the sample holding flow path. Thus, the sample is held within the sample holding flow path. When the switching unit is in the second state, the mobile phase pumped out is guided from the first port to the sample holding flow path. Thus, the sample held within the sample holding flow path, together with the mobile phase, is guided to the column flow path. The sample guided to the column flow path is then guided to the discharge port after being separated by substance. In this way, until the mobile phase is guided to the column flow path, since unnecessary flow paths such as piping are not used, sample diffusion within the mobile phase can be suppressed.

[0189] (Item 12) Liquid chromatographs involving other schemes, having:

[0190] The microfluidic device described in item 11;

[0191] The pump;

[0192] The sample filling portion; and

[0193] The detection unit.

[0194] The liquid chromatograph described in item 12 is capable of suppressing the diffusion of the sample in the mobile phase.

Claims

1. A microfluidic device, characterized in that, It has a body section consisting of one or more components. The body portion includes: The connecting part can be connected to the separation column; and Multiple flow paths The multiple flow paths include: Sample holding section, for holding the sample; and The inlet flow path enables the sample to be introduced into the separation column for sample separation. The microfluidic device further comprises: The switching component is slidably connected to the main body and can selectively connect the multiple flow paths by sliding relative to the main body.

2. The microfluidic device according to claim 1, characterized in that, The connecting part is a prism shape extending in one direction. The microfluidic device further comprises: The sleeve is penetrated by the connecting part, has an inner circumferential surface that can engage with the connecting part, and has a cylindrical outer circumferential surface. The nut has a first through hole through which the sleeve passes and a first threaded groove that can engage with a main threaded groove formed on the inlet port of the separating column; as well as The ferrule has a second through hole through which the sleeve passes and can seal the flow path within the separation column.

3. The microfluidic device according to claim 1, characterized in that, The connecting part is a cylindrical shape extending in one direction. The microfluidic device further comprises: The nut has a first through hole through which the connecting part passes and a first threaded groove that can engage with the main threaded groove formed on the inlet port of the separating column; as well as The sleeve has a second through hole through which the connecting part passes and can seal the flow path within the separating column.

4. The microfluidic device according to claim 1, characterized in that, Further features include: The nut has a first threaded groove capable of engaging with a main threaded groove formed on the inlet port of the separating column. The connecting part includes: The first cylindrical component extends in one direction; and The second cylindrical component extends further from the first cylindrical component in the direction and has a larger diameter than the first cylindrical component, and has a sealing surface intersecting the direction. The nut contains: The first through hole allows the first cylindrical component to pass through; and The gap extends in the said direction and allows the first cylindrical component to be installed into the first through hole.

5. The microfluidic device according to claim 1, characterized in that, Furthermore, it includes a column support mechanism. The connecting part is a column shape extending in one direction. The separating column has a port for connecting to the connecting portion, and a protrusion protruding outward around the port. The column support mechanism includes: A receiving component has an insertion hole for inserting the protrusion of the separating post, and a receiving space for receiving the protrusion; and The pressing part is fixed to the connecting part within the receiving space and is configured to press the protrusion onto the receiving member so that the receiving member engages with the protrusion.

6. The microfluidic device according to any one of claims 1 to 5, characterized in that, The multiple components are plate-shaped.

7. The microfluidic device according to any one of claims 1 to 5, characterized in that, The switching component can be switched to: The state of connection between the sample holding part and the inlet flow path; and The sample holding section and the inlet flow path are not connected.

8. A liquid chromatograph, characterized in that, have: The microfluidic device according to any one of claims 1 to 5, wherein the plurality of flow paths further comprises: The first port is connected to the pump that delivers the mobile phase; and The second port is connected to the sample filling section for filling the sample. The switching component can be switched to: The first state of connecting the second port and the sample holding part; and The second state connecting the first port, the sample holding part, and the inlet flow path; The pump; The sample filling section; The separation column; and The detection department detects the components that have passed through the separation column.

9. A connecting device for connecting the microfluidic device, the separation column, and the detector as described in claim 1, characterized in that, have: The first fixing component is capable of fixing the microfluidic device and connecting the connecting portion of the microfluidic device to the inlet port of the separation column; The second fixing component is capable of fixing the detector and can be connected to the inlet port of the detector and the outlet port of the separation column; The guide rail is configured to allow at least one of the first fixing member and the second fixing member to move relative to the other. as well as The clamping mechanism is capable of reducing the distance between the first fixing component and the second fixing component disposed on the guide rail.

10. A microfluidic device, characterized in that, have: The main body has multiple flow paths; and A switching component is slidably connected to the main body and can selectively connect the multiple flow paths of the main body by sliding relative to the main body. The body portion includes: First plate-shaped component; and Second plate-shaped component, The multiple flow paths include: A sample retention flow path is formed in the first plate-shaped component and retains the sample; and The chromatographic column flow path is formed in the second plate-shaped component and is capable of separating the sample by components.

11. The microfluidic device according to claim 10, characterized in that, The body portion further includes: The third plate-shaped component, The third plate-shaped component includes a connecting portion that can be connected to a detection portion for detecting components that have passed through the chromatographic column flow path. The multiple flow paths include: The first port is connected to the pump that delivers the mobile phase; The second port is connected to the sample filling section filled with the sample; and An outlet port, formed in the connecting portion, discharges components that have passed through the chromatographic column flow path to the detection portion. The switching component can be switched to: The second port and the sample maintain the first state of the flow path; and The second state connecting the first port, the sample holding flow path, the chromatographic column flow path, and the ejection port.

12. A liquid chromatograph, characterized in that, have: The microfluidic device as described in claim 11; The pump; The sample filling portion; and The detection unit.

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