A method for artificial cultivation of cordyceps sinensis by light induction

Abstract

This invention relates to the field of edible fungi cultivation technology, and particularly to a method for artificially cultivating Cordyceps sinensis using light-induced induction. The method comprises the following steps: (1) During the basal differentiation stage of Cordyceps sinensis, providing 200-650 lux of light for 15-25 days; (2) During the fruiting body growth stage of Cordyceps sinensis, providing 1000-1500 lux of light for 30-45 days; (3) When the fruiting body reaches a length of 3-5 cm, providing 500-800 lux of light for 10-15 days. This invention utilizes customized LED lights under specific illumination for the growth of Cordyceps sinensis fruiting bodies, significantly improving fruiting body development and the content of cordycepin and adenosine. This provides a basis for the artificial cultivation of Cordyceps sinensis.

Description

Technical Field

[0001] This invention relates to the field of edible fungi cultivation technology, and in particular to a method for artificially cultivating Cordyceps sinensis using light-induced induction. Background Technology

[0002] Cordyceps sinensis is a fungus-insect complex formed when the Cordyceps sinensis fungus infects the larvae of the ghost moth. It possesses extremely high medicinal and economic value. However, under artificial cultivation conditions, there are technical bottlenecks, such as uneven development of the fruiting bodies, thin stromata, and variations in stroma color. These bottlenecks severely restrict the large-scale artificial cultivation of Cordyceps sinensis. Therefore, it is urgent to find methods to regulate the development of Cordyceps sinensis fruiting bodies, thereby achieving precise and efficient control over the quality and yield of Cordyceps sinensis. Summary of the Invention

[0003] The purpose of this invention is to provide a method for artificially cultivating Cordyceps sinensis using light-induced induction.

[0004] To achieve the above-mentioned objectives, the present invention provides the following technical solution: This invention provides a method for artificially cultivating Cordyceps sinensis using light-induced induction, comprising the following steps: (1) During the basal differentiation period of Cordyceps sinensis, provide light of 200~650 lux and culture for 15~25 days; (2) During the fruiting body growth period of Cordyceps sinensis, provide 1000~1500 lux of light and cultivate for 30~45 days; (3) When the fruiting body of Cordyceps sinensis reaches 3-5cm in length, provide 500-800 lux of light and cultivate for 10-15 days.

[0005] Preferably, the illumination described in steps (1) to (3) is provided by the LED spectrum; The light source of the LED spectrum includes one or more of yellow light, blue light, and violet light.

[0006] Preferably, the light cycle during the cultivation in step (1) is 10-14 hours of light / 10-14 hours of darkness.

[0007] Preferably, the light cycle during the cultivation in step (2) is 12-16 hours of light / 8-12 hours of darkness.

[0008] Preferably, the humidity of the culture in step (3) is 70-80%.

[0009] The present invention also provides the application of the artificial cultivation method described above in improving the quality of Cordyceps sinensis.

[0010] This invention also provides the application of the aforementioned artificial cultivation method in increasing the content of cordycepin and adenosine in Cordyceps sinensis.

[0011] The present invention has the following advantages: 1. Significantly increased active ingredients LED blue light and blue-violet combined light can significantly promote the accumulation of active ingredients such as cordycepin, adenosine, cordycepic acid, and cordyceps polysaccharides, with the content reaching or exceeding the level of wild cordyceps.

[0012] 2. Better fruiting body development

[0013] Phased and precise light control effectively solves problems such as uneven development, thinness, and deformity of fruiting bodies, significantly improving the uniformity of fruiting bodies and the yield of finished products.

[0014] 3. Significant cost reduction and efficiency improvement

[0015] LED light sources have high photoelectric conversion efficiency, low heat generation, and long service life. They save more than 50% energy compared to traditional light sources, making them suitable for large-scale industrial cultivation. Attached Figure Description

[0016] Figure 1 Cordyceps sinensis fruiting bodies cultured under ordinary blue light; Figure 2 Cordyceps sinensis fruiting bodies cultured under LED blue light + violet light; Figure 3 Cordyceps sinensis fruiting bodies cultured under LED yellow light; Figure 4 Cordyceps sinensis fruiting bodies cultured under ordinary yellow light; Figure 5 It serves as a cultivation medium for Cordyceps sinensis and represents the larval stage (hepialus moth larvae). Figure 6 The fruiting body of Cordyceps sinensis obtained in Example 2. Detailed Implementation

[0017] This invention provides a method for artificially cultivating Cordyceps sinensis using light-induced induction, comprising the following steps: (1) During the basal differentiation period of Cordyceps sinensis, provide light of 200~650 lux and culture for 15~25 days; (2) During the fruiting body growth period of Cordyceps sinensis, provide 1000~1500 lux of light and cultivate for 30~45 days; (3) When the fruiting body of Cordyceps sinensis reaches 3-5cm in length, provide 500-800 lux of light and cultivate for 10-15 days.

[0018] In this invention, Cordyceps sinensis worms with consistent growth status are selected, with mycelium covering the worm body, the worm body is plump, and there is no contamination from other bacteria.

[0019] In this invention, light is the most critical factor inducing fruiting body formation and influencing quality. Insufficient light can lead to fruiting body failure or deformed morphology, while excessive light can inhibit growth or even cause sunburn. Customized LED light spectrum can more precisely meet the needs of different growth stages of Cordyceps sinensis. Customized LED light energy can significantly improve the development of Cordyceps sinensis fruiting bodies and significantly increase the content of cordycepin and adenosine.

[0020] In this invention, the illumination described in steps (1) to (3) is provided by the LED spectrum; The light source of the LED spectrum includes one or more of yellow light, blue light, and violet light.

[0021] The wavelength of the yellow light is 580~595nm, the wavelength of the blue light is 450~470nm, and the wavelength of the violet light is 400~420nm.

[0022] In this invention, the light cycle during cultivation in step (1) is 10-14 hours of light / 10-14 hours of darkness, preferably 12 hours of light / 12 hours of darkness.

[0023] In this invention, the light cycle during cultivation in step (2) is 12-16 hours of light / 8-12 hours of darkness, preferably 14 hours of light / 10 hours of darkness.

[0024] In this invention, the humidity of the culture in step (3) is 70-80%, preferably 75%.

[0025] The present invention also provides the application of the artificial cultivation method described above in improving the quality of Cordyceps sinensis.

[0026] This invention also provides the application of the aforementioned artificial cultivation method in increasing the content of cordycepin and adenosine in Cordyceps sinensis.

[0027] In this invention, the light source of the LED spectrum is preferably a combination of blue light and violet light, and the light intensity ratio of blue light to violet light is preferably 2:1.

[0028] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0029] In this invention, the wavelength of the yellow light is 580~595nm, the wavelength of the blue light is 450~470nm, and the wavelength of the violet light is 400~420nm.

[0030] The Cordyceps strain in this embodiment of the invention has the Latin name Ophiocordyceps sinensis The Chinese name is Cordyceps sinensis (Hepialus chinensis, Cordyceps sinensis), strain number SHIMCC D13346. It was purchased from the Shanghai Microbial Culture Collection Center (SHMCC).

[0031] The Cordyceps sinensis larvae in this embodiment of the invention are ghost moth larvae, such as... Figure 5 As shown.

[0032] Example 1

[0033] Light source selection

[0034] Select Cordyceps sinensis larvae in uniform growth stages and inoculate them with Cordyceps sinensis strains, ensuring the mycelium covers the larvae, the larvae are plump, and free from contamination. During the differentiation stage, provide 500 lux of light with a photoperiod of 12 hours of light and 12 hours of darkness for 20 days until the fruiting body primordia form. During the fruiting body growth stage, provide 1200 lux of light with a photoperiod of 14 hours of light and 10 hours of darkness for 40 days until the fruiting body reaches a length of 3-5 cm, indicating the maturity of the stroma. During the stroma maturity stage, maintain an ambient humidity of 75% and provide 600 lux of light for 12 days before harvesting.

[0035] The illumination is provided by LED spectra from different light sources, including blue light, yellow light, and violet light.

[0036] Use ordinary blue light and ordinary yellow light as controls.

[0037] Cordyceps sinensis fruiting bodies were harvested, and the effects of different light sources on the cordycepin and adenosine content of Cordyceps sinensis were investigated. The results are shown in Table 1. The fruiting bodies of Cordyceps sinensis cultured under different light sources are as follows: Figures 1-4 As shown, when combining blue and violet LED light, the preferred ratio of blue to violet light intensity is 2:1.

[0038] Table 1. Effects of different light sources on Cordyceps sinensis

[0039] It is evident that the light source provided by LED lights has a significant impact on the growth of Cordyceps sinensis fruiting bodies, and the light source provided by LED lights can effectively increase the content of cordycepin and adenosine in Cordyceps sinensis. This provides a basis for the artificial cultivation of Cordyceps sinensis.

[0040] Example 2

[0041] Low intensity light group

[0042] Select Cordyceps sinensis larvae in uniform growth stages and inoculate them with Cordyceps sinensis strains, ensuring the mycelium covers the larvae, the larvae are plump, and free from contamination. During the differentiation stage, provide 200 lux of light with a photoperiod of 12 hours of light and 12 hours of darkness for 22 days until the fruiting body primordia form. During the fruiting body growth stage, provide 1000 lux of light with a photoperiod of 14 hours of light and 10 hours of darkness for 41 days until the fruiting body reaches a length of 3-5 cm, indicating the maturity of the stroma. During the stroma maturity stage, maintain an ambient humidity of 75% and provide 500 lux of light for 15 days before harvesting.

[0043] The illumination was provided by LEDs from different light sources, specifically a mixture of blue and violet light at a 2:1 intensity ratio. The cultivated Cordyceps sinensis fruiting bodies are shown below. Figure 6 As shown.

[0044] The fruiting bodies of Cordyceps sinensis were harvested, and the contents of cordycepin and adenosine in the fruiting bodies were determined. The results showed that the cordycepin content was 3.8 mg / g and the adenosine content was 2.0 mg / g.

[0045] Example 3

[0046] Medium and high intensity light group

[0047] Select Cordyceps sinensis larvae in uniform growth stages and inoculate them with Cordyceps sinensis strains, ensuring the mycelium covers the larvae, the larvae are plump, and free from contamination. During the differentiation stage, provide 650 lux of light with a photoperiod of 12 hours of light and 12 hours of darkness for 18 days until the fruiting body primordia form. During the fruiting body growth stage, provide 1500 lux of light with a photoperiod of 14 hours of light and 10 hours of darkness for 35 days until the fruiting body reaches a length of 3-5 cm, indicating the maturity of the stroma. During the stroma maturity stage, maintain an ambient humidity of 75% and provide 800 lux of light for 12 days before harvesting.

[0048] The illumination is provided by LED spectra from different light sources, namely blue light and violet light mixed at a light intensity ratio of 2:1.

[0049] The fruiting bodies of Cordyceps sinensis were harvested, and the contents of cordycepin and adenosine were determined. The results showed that the cordycepin content was 4.3 mg / g and the adenosine content was 2.8 mg / g.

[0050] As shown in the above embodiments, this invention provides a method for artificially cultivating Cordyceps sinensis using light-induced methods. This invention utilizes customized LED lights under specific illumination to promote the growth of Cordyceps sinensis fruiting bodies, which can significantly improve the development of the fruiting bodies and the content of cordycepin and adenosine. This provides a basis for the artificial cultivation of Cordyceps sinensis.

[0051] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A method for artificially cultivating Cordyceps sinensis using light-induced induction, characterized in that, Includes the following steps: (1) During the basal differentiation period of Cordyceps sinensis, provide light of 200~650 lux and culture for 15~25 days; (2) During the fruiting body growth period of Cordyceps sinensis, provide 1000~1500 lux of light and cultivate for 30~45 days; (3) When the fruiting body of Cordyceps sinensis reaches 3-5cm in length, provide 500-800 lux of light and cultivate for 10-15 days.

2. The artificial breeding method according to claim 1, characterized in that, The illumination described in steps (1) to (3) is provided by the LED spectrum; The light source of the LED spectrum includes one or more of yellow light, blue light, and violet light.

3. The artificial breeding method according to claim 1, characterized in that, The light cycle during cultivation in step (1) is 10-14 hours of light / 10-14 hours of darkness.

4. The artificial breeding method according to claim 1, characterized in that, The light cycle during cultivation in step (2) is 12-16 hours of light and 8-12 hours of darkness.

5. The artificial breeding method according to claim 1, characterized in that, The humidity for cultivation in step (3) is 70-80%.

6. The application of the artificial cultivation method according to any one of claims 1 to 5 in improving the quality of Cordyceps sinensis.

7. The application of the artificial cultivation method according to any one of claims 1 to 5 in increasing the content of cordycepin and adenosine in Cordyceps sinensis.

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