Long-acting radix isatidis and radix scrophulariae compound and preparation method thereof

Abstract

This invention provides a long-acting dandelion and indigo herbal compound and its preparation method. The long-acting dandelion and indigo herbal compound comprises the following components: herbal extract, adhesive microparticles, adjuvants, and deionized water. This long-acting dandelion and indigo herbal compound treats infectious bursal disease in chickens, achieving high survival rates and improving production performance. It also provides long-term prevention of infectious bursal disease in chickens. The herbal components are non-toxic, do not induce drug resistance, and leave no drug residues.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese veterinary medicine technology, and in particular to a long-acting dandelion and indigo compound and its preparation method. Background Technology

[0002] Infectious bursal disease (IBD) in chickens is one of the most common and damaging diseases in the poultry industry. First discovered in the late 1950s, it has since spread widely globally. IBD virus is highly contagious and can be transmitted through various routes, such as contact, airborne transmission, and contaminated feed and water. Its impact on the poultry industry is mainly reflected in the following aspects: First, it causes mass mortality in chickens, especially chicks, with mortality rates reaching over 30%, resulting in severe economic losses for farmers. Second, recovered chickens may experience immunosuppression, reducing their resistance to other diseases and making them more susceptible to secondary infections, further exacerbating the damage.

[0003] In terms of treatment, traditional methods include using vaccines for prevention and immune enhancers to improve the immunity of chickens. However, with the continuous mutation of viruses and the emergence of drug resistance, relying solely on these methods is sometimes not ideal. In recent years, traditional Chinese medicine (TCM) treatment has gradually gained attention. TCM has advantages such as multi-target therapy and fewer side effects. Some TCM herbs with heat-clearing, detoxifying, and pathogen-eliminating effects have been used to treat this disease with some success. Meanwhile, research is also dedicated to exploring new treatment methods and drugs, such as the development of novel antiviral drugs.

[0004] Furthermore, environmental control and husbandry management play a crucial role in the prevention and treatment of infectious bursal disease (IBD) in chickens. Maintaining cleanliness and hygiene in the chicken house, reasonable stocking density, and good ventilation can all effectively reduce the risk of disease outbreaks. In conclusion, research and treatment of IBD in chickens are constantly evolving to address the ongoing challenges it poses.

[0005] Currently, patent number CN101332256B discloses a traditional Chinese medicine composition and preparation method for infectious bursal disease (IBD) in chickens. This composition boasts a protection rate of up to 88.82% against IBD, an effective treatment rate of 92% in the field, and a significant efficacy rate exceeding 85%. Patent number CN103599447B discloses a traditional Chinese medicine immunomodulator for the prevention of IBD in chickens. This immunomodulator demonstrates significant efficacy in preventing IBD and possesses advantages such as being non-toxic, preventing drug resistance, and leaving no drug residues. However, current traditional Chinese medicines tend to have a relatively slow onset of action, which may not be sufficient to quickly control disease progression in urgent situations. Furthermore, the duration of prevention and treatment for IBD in chickens is short, and recurrence is possible.

[0006] In view of the problems existing in the prior art, this application aims to solve the problems of relatively slow efficacy of traditional Chinese medicine in treating infectious bursal disease in chickens, low survival rate, low production performance, rapid metabolism of Western medicine, drug resistance, and poor effect in preventing infectious bursal disease in chickens. Summary of the Invention

[0007] Purpose of the invention:

[0008] The purpose of this invention is to provide a long-acting dandelion and indigo compound and its preparation method, which can rapidly treat infectious bursal disease in chickens, with a high survival rate and improved production performance. At the same time, it can prevent infectious bursal disease in chickens for a long time. The Chinese medicine ingredients are non-toxic, do not cause drug resistance, and have no drug residues.

[0009] The technical solution of the present invention:

[0010] This application provides a long-acting dandelion and indigo traditional Chinese medicine compound, characterized in that the long-acting dandelion and indigo traditional Chinese medicine compound comprises the following components: traditional Chinese medicine extract, adhesive microparticles, adjuvants, and deionized water.

[0011] Furthermore, the long-acting dandelion and indigo herbal compound contains, by weight, 100-200 parts of herbal extract, 20-50 parts of adhesive microparticles, 15-30 parts of adjuvants, and 1000-3000 parts of deionized water.

[0012] Furthermore, the herbal extract contains the following components: dandelion, isatis root, indigo leaf, honeysuckle, andrographis paniculata, houttuynia cordata, astragalus, scrophularia ningpoensis, rehmannia root, lysimachia christinae, dictamnus dasycarpus root bark, gentiana macrophylla, and anemarrhena asphodeloides.

[0013] Furthermore, the raw materials for preparing the adhesive microparticles include: carboxymethyl hydroxypropyl cellulose, chitosan, taurine, and alginate.

[0014] The method for preparing the adhesive microparticles includes the following steps:

[0015] (1) Weigh the carboxymethyl hydroxypropyl cellulose and chitosan and add them to the reaction vessel. Add deionized water and stir until the mixture is homogeneous to obtain a mixed solution.

[0016] (2) Raise the temperature of the reactor to 40-60°C, add taurine and alginate to make the pH of the reaction system 5-7.5, stir slowly, and react for 2-4 hours to obtain the reaction solution;

[0017] (3) After the reaction is completed, the reaction solution is extracted, separated and dried to obtain the adhesive microparticles.

[0018] Furthermore, the mass ratio of carboxymethyl hydroxypropyl cellulose to chitosan is 1:4 to 8.

[0019] The preparation method of the traditional Chinese medicine extract includes the following steps:

[0020] S1: Wash, dry, and chop the following medicinal materials: dandelion, isatis root, indigo leaf, honeysuckle, andrographis paniculata, houttuynia cordata, astragalus, scrophularia ningpoensis, rehmannia glutinosa, lysimachia christinae, dictamnus dasycarpus, gentiana macrophylla, and anemarrhena asphodeloides.

[0021] S2: Place the medicinal herbs dandelion, isatis root, indigo leaf, honeysuckle, andrographis paniculata, houttuynia cordata, astragalus, scrophularia, rehmannia root, lysimachia christinae, dictamnus dasycarpus root bark, gentian root, and anemarrhena rhizome into a clay pot, add an appropriate amount of water, and soak for 0.5 to 1 hour to fully moisten the herbs.

[0022] S3: First, bring the soaked herbs to a boil over high heat at 90-100℃, then reduce to low heat at 50-60℃ and simmer for 0.5-1 hour, stirring occasionally during the process.

[0023] S4: Filter the decocted herbs through gauze to extract the liquid and dregs. Add an appropriate amount of water to the dregs again and repeat the above decoction and filtration steps. Combine the two liquids to obtain a Chinese herbal extract.

[0024] Furthermore, the components of the herbal extract, by weight, include: 250-300 parts dandelion, 220-280 parts isatis root, 150-180 parts indigo leaf, 120-150 parts honeysuckle, 50-100 parts andrographis paniculata, 50-80 parts houttuynia cordata, 150-200 parts astragalus, 80-120 parts scrophularia, 100-150 parts rehmannia root, 80-120 parts lysimachia christinae, 80-100 parts dictamnus dasycarpus root bark, 50-80 parts gentian root, and 80-100 parts anemarrhena rhizome.

[0025] Furthermore, the adjuvant is potassium sorbate, potassium iodide, β-cyclodextrin, or Tween-80.

[0026] This application also provides a method for preparing a long-acting dandelion and indigo compound, characterized by comprising the following steps:

[0027] Step 1: Place the herbal extract in a container, control the temperature at 80-90℃, and concentrate the herbal extract to a concentration with a relative density of 1.2-1.5;

[0028] Step 2: Add the adhesive microparticles and adjuvants to the concentrate in sequence and stir until homogeneous to obtain a mixture of traditional Chinese medicines;

[0029] Step 3: Prepare different concentrations of the Chinese herbal medicine mixture with deionized water, and then sterilize it using pasteurization to obtain a long-acting dandelion and indigo Chinese herbal medicine compound.

[0030] This invention provides a long-acting dandelion and indigo herbal compound, which utilizes a decoction method to extract the herbal extract. The herbal extract is prepared by mixing and decocting herbs with different effects. Among them, dandelion, indigo leaf, honeysuckle, isatis root, andrographis paniculata, and houttuynia cordata not only have the effects of clearing heat and detoxifying, cooling blood and eliminating spots, dispersing wind-heat, eliminating carbuncles and draining pus, and promoting diuresis, but also have antibacterial and antiviral effects, inhibiting viral activity and treating viral infections; astragalus has the effects of tonifying qi and raising yang, consolidating the exterior and stopping sweating, and can enhance the body's immune function; scrophularia and rehmannia have the effects of clearing heat and cooling blood, nourishing yin and reducing fire, detoxifying and dispersing nodules, and nourishing yin and generating fluids; lysimachia christinae has the effects of promoting diuresis and relieving jaundice, promoting diuresis and relieving strangury, and detoxifying and reducing swelling; dictamnus dasycarpus and gentian have the effects of dispelling wind and dampness, clearing damp heat, relieving pain, and reducing deficiency heat; anemarrhena asphodeloides can clear heat and purge fire, nourish yin and moisten dryness.

[0031] Chitosan acts as a carrier, supporting carboxymethyl hydroxypropyl cellulose to enhance adhesion. It also exhibits inhibitory effects on various bacteria and fungi and regulates the immune system. Adhesive microparticles physically adhere to the surface of the gastrointestinal mucosa, prolonging the drug's residence time at the site of action. This allows for sustained and slow drug release, resulting in a prolonged therapeutic effect. Secondly, adhesion reduces the likelihood of the drug being rapidly expelled from the body due to gastrointestinal motility, thus maintaining a relatively stable drug concentration and achieving a long-lasting effect. Furthermore, this adhesion allows the drug to make more thorough contact with the absorption site, promoting drug absorption and utilization, thereby prolonging the duration of action. In addition, adhesive microparticles may protect the drug from degradation by the gastrointestinal environment (such as gastric acid and digestive enzymes), ensuring that the drug can exert its effects in a more intact form for a longer period.

[0032] Beneficial effects:

[0033] This invention provides a long-acting dandelion and indigo compound that can rapidly treat infectious bursal disease in chickens, with a high survival rate and improved production performance. It can also provide long-term prevention of infectious bursal disease in chickens. The herbal ingredients are non-toxic, do not cause drug resistance, and have no drug residues. The raw materials are inexpensive and readily available, making it a promising candidate for development. Detailed Implementation

[0034] The present invention will be described below with reference to specific embodiments. It should be noted that the following embodiments are examples of the present invention and are used only to illustrate the invention, not to limit it. Other combinations and various modifications within the scope of the present invention can be made without departing from its spirit or scope.

[0035] Unless otherwise specified, all chemical reagents used in this invention are commercially available analytical grade reagents.

[0036] Preparation of Adhesive Particle A:

[0037] (1) Add 1g of carboxymethyl hydroxypropyl cellulose and 5g of chitosan to the reaction vessel, add 300ml of deionized water, stir evenly, and prepare a mixed solution.

[0038] (2) Raise the temperature of the reactor to 50°C, add 0.5g taurine and 0.2g alginate to make the pH of the reaction system 6, stir slowly, and react for 4 hours to obtain the reaction solution;

[0039] (3) After the reaction was completed, the reaction solution was extracted with 150 mL of dichloromethane, separated by a separatory funnel, and dried at 40 °C to obtain the adhesive microparticles.

[0040] Preparation of Adhesive Particle B:

[0041] The difference between this preparation and the adhesive microparticle A is that 5g of chitosan is replaced with 8g of chitosan.

[0042] Preparation of Adhesive Microparticle C:

[0043] The difference between this preparation and the adhesive microparticle A is that 5g of chitosan is replaced with 2g of chitosan.

[0044] Preparation of Adhesive Particles D:

[0045] The difference between this preparation and the adhesive microparticle A is that 5g of chitosan is replaced with 10g of chitosan.

[0046] Preparation of herbal extract A:

[0047] S1: Wash, dry, and chop the following medicinal materials: dandelion, isatis root, indigo leaf, honeysuckle, andrographis paniculata, houttuynia cordata, astragalus, scrophularia ningpoensis, rehmannia glutinosa, lysimachia christinae, dictamnus dasycarpus, gentiana macrophylla, and anemarrhena asphodeloides.

[0048] S2: Place 25g of dandelion, 25g of isatis root, 15g of indigo leaf, 15g of honeysuckle, 8g of andrographis paniculata, 6g of houttuynia cordata, 18g of astragalus, 10g of scrophularia, 12g of rehmannia root, 8g of lysimachia christinae, 8g of dictamnus dasycarpus root bark, 5g of gentian root, and 8g of anemarrhena rhizome into a clay pot, add water to submerge the herbs by 5cm, and soak for 0.5 hours to fully moisten the herbs;

[0049] S3: First bring the soaked herbs to a boil over high heat at 100°C, then reduce to low heat at 75°C and simmer for 1 hour, stirring occasionally during the process.

[0050] S4: Filter the decocted herbs through gauze to extract the liquid and dregs. Add water to the dregs again, submerging them by 3cm. Repeat the above decoction and filtration steps, and combine the two liquids to obtain Chinese herbal extract A.

[0051] Preparation of herbal extract B:

[0052] The difference between this preparation and the traditional Chinese medicine extract A is that in step S2, 25g dandelion, 25g isatis root, 15g indigo leaf, 15g honeysuckle, 8g andrographis paniculata, and 6g houttuynia cordata are replaced with 10g dandelion, 15g isatis root, 25g indigo leaf, 30g honeysuckle, 6g andrographis paniculata, and 8g houttuynia cordata, respectively.

[0053] Preparation of herbal extract C:

[0054] The difference between this preparation and the Chinese herbal extract A is that in step S2, 18g Astragalus membranaceus, 10g Scrophularia ningpoensis, 12g Rehmannia glutinosa, 8g Lysimachia christinae, 8g Dictamnus dasycarpus, 5g Gentiana macrophylla, and 8g Anemarrhena asphodeloides are replaced with 10g Astragalus membranaceus, 15g Scrophularia ningpoensis, 5g Rehmannia glutinosa, 17g Lysimachia christinae, 15g Dictamnus dasycarpus, 2g Gentiana macrophylla, and 5g Anemarrhena asphodeloides, respectively.

[0055] Preparation method of long-acting dandelion and indigo compound:

[0056] Step 1: Place the herbal extract in a container, control the temperature at 80℃, and concentrate the herbal extract to a concentration with a relative density of 1.2.

[0057] Step 2: Add the adhesive microparticles and adjuvants to the concentrate in sequence and stir until homogeneous to obtain a mixture of traditional Chinese medicines;

[0058] Step 3: Prepare a 1mg / mL solution by mixing the Chinese herbal medicine mixture with deionized water, and then sterilize it using pasteurization to obtain a long-acting dandelion and indigo Chinese herbal medicine compound.

[0059] Examples 1-6

[0060] According to the above-mentioned preparation method of the long-acting dandelion and indigo traditional Chinese medicine compound, Table 1 is the ingredient list of the long-acting dandelion and indigo traditional Chinese medicine compound in Examples 1 to 6.

[0061] Table 1

[0062]

[0063]

[0064] Comparative Examples 1-4:

[0065] According to the above-mentioned preparation method of the long-acting dandelion and indigo traditional Chinese medicine compound, Table 2 shows the ingredient list of the long-acting dandelion and indigo traditional Chinese medicine compound for comparative examples 1 to 4.

[0066] Table 2

[0067]

[0068] Test experiment:

[0069] Using the methods provided in Examples 1-8 and Comparative Examples 1-4, samples of traditional Chinese medicine compound preparations were prepared. Then, 200,000 broiler chickens were selected from a breeding base. At 28 days of age, some chickens exhibited a disease characterized by lethargy, ruffled feathers, drooping heads, drowsiness with closed eyes, pecking at their vents, and excreting white, sticky, watery feces. The disease was diagnosed as infectious bursal disease (IBD) by the Animal Disease Quarantine Center of the College of Animal Science and Veterinary Medicine.

[0070] The diseased broilers were randomly divided into 12 groups of 50 birds each. Groups 1-12 were treated with the traditional Chinese medicine compound samples provided in Examples 1-8 and Comparative Examples 1-4, respectively, by intramuscular injection at a rate of 1 mg / mL / day per chicken. After 3 days of treatment, the treatment was discontinued, and the cure rate of each group was calculated. The survival rate of each group was then calculated after 30 days of observation. The average egg production of the cured hens over 30 days was also calculated to observe the production performance of the cured chickens. The results are shown in Table 3.

[0071] Table 3:

[0072] Example 1 97.5 95.5 15 Example 2 96.9 95.6 15 Example 3 96.1 95.2 15 Example 4 93.0 92.5 14 Example 5 92.2 92.6 14 Example 6 85.2 85.6 12 Example 7 83.2 88.2 12 Example 8 81.2 82.3 12 Comparative Example 1 15 10 6 Comparative Example 2 62.3 64.2 10 Comparative Example 3 92.4 69.5 10 Comparative Example 4 95.1 74.2 10

[0073] The data above shows that the long-acting dandelion and bluewort herbal compound prepared by this invention contains a variety of medicinal herbs with different effects. After grinding and mixing these herbs to extract plant extracts, the resulting dandelion and bluewort herbal compound, along with adhesive microparticles, adjuvants, and deionized water, exhibits rapid efficacy, high treatment rate, and good therapeutic effect. It also enhances production performance and can prevent infectious bursal disease in chickens. Specifically, comparing Examples 4-5 with Example 1 shows that an unreasonable ratio of medicinal herbs in the preparation of the herbal extract can lead to a decrease in the treatment rate, preventative ability, and production performance of the prepared long-acting dandelion and bluewort herbal compound. Comparing Examples 6-8 with Example 1 shows that an unreasonable ratio of chitosan to carboxymethyl hydroxypropyl cellulose in the preparation of the adhesive microparticles can also lead to a decrease in these effects. Reasonable addition of traditional Chinese medicine extracts can lead to a decrease in the preventive ability and production performance of the prepared long-acting dandelion and indigo herbal compound. A comparison between Comparative Example 1 and Example 1 shows that the absence of the herbal extract is essentially ineffective in treating infectious bursal disease in chickens. A comparison between Comparative Example 2 and Example 1 shows that the treatment rate, preventive ability, and production performance of the long-acting dandelion and indigo herbal compound without the addition of adhesive microparticles are worse. A comparison between Comparative Example 3 and Example 1 shows that the preventive ability and production performance of the long-acting dandelion and indigo herbal compound prepared by replacing the adhesive microparticles with xanthan gum are worse. A comparison between Comparative Example 4 and Example 3 shows that the preventive ability and production performance of the long-acting dandelion and indigo herbal compound prepared by replacing the adhesive microparticles with hydroxypropyl methylcellulose are worse.

[0074] This invention can also be implemented in various other ways. Without departing from the spirit and essence of this invention, those skilled in the art can make various corresponding changes and modifications according to this invention, but these corresponding changes and modifications should all fall within the protection scope of the appended claims.

Claims

1. A long-acting dandelion and indigo herbal compound, characterized in that, The long-acting dandelion and indigo herbal compound contains the following components: herbal extract, adhesive microparticles, adjuvants, and deionized water.

2. The long-acting dandelion and indigo compound according to claim 1, characterized in that, The long-acting dandelion and indigo herbal compound contains, by weight, 100-200 parts of herbal extract, 20-50 parts of adhesive microparticles, 15-30 parts of adjuvants, and 1000-3000 parts of deionized water.

3. The long-acting dandelion and indigo compound according to claim 2, characterized in that, The herbal extract contains the following components: dandelion, isatis root, indigo leaf, honeysuckle, andrographis paniculata, houttuynia cordata, astragalus, scrophularia ningpoensis, rehmannia root, lysimachia christinae, dictamnus dasycarpus root bark, gentiana macrophylla, and anemarrhena asphodeloides.

4. The long-acting dandelion and indigo herbal compound according to claim 1, characterized in that, The raw materials for preparing the adhesive microparticles include: carboxymethyl hydroxypropyl cellulose, chitosan, taurine, and alginate.

5. The long-acting dandelion and indigo herbal compound according to claim 4, characterized in that, The method for preparing the adhesive microparticles includes the following steps: (1) Weigh the carboxymethyl hydroxypropyl cellulose and chitosan and add them to the reaction vessel. Add deionized water and stir until the mixture is homogeneous to obtain a mixed solution. (2) Raise the temperature of the reactor to 40-60°C, add taurine and alginate to make the pH of the reaction system 5-7.5, stir slowly, and react for 2-4 hours to obtain the reaction solution; (3) After the reaction is completed, the reaction solution is extracted, separated and dried to obtain the adhesive microparticles.

6. The long-acting dandelion and indigo herbal compound according to claim 4, characterized in that, The mass ratio of carboxymethyl hydroxypropyl cellulose to chitosan is 1:4 to 8.

7. The long-acting dandelion and indigo herbal compound according to claim 1, characterized in that, The preparation method of the traditional Chinese medicine extract includes the following steps: S1: Wash, dry, and chop the following medicinal materials: dandelion, isatis root, indigo leaf, honeysuckle, andrographis paniculata, houttuynia cordata, astragalus, scrophularia ningpoensis, rehmannia glutinosa, lysimachia christinae, dictamnus dasycarpus, gentiana macrophylla, and anemarrhena asphodeloides. S2: Place the medicinal herbs dandelion, isatis root, indigo leaf, honeysuckle, andrographis paniculata, houttuynia cordata, astragalus, scrophularia, rehmannia root, lysimachia christinae, dictamnus dasycarpus root bark, gentian root, and anemarrhena rhizome into a clay pot, add an appropriate amount of water, and soak for 0.5 to 1 hour to fully moisten the herbs. S3: First, bring the soaked herbs to a boil over high heat at 90-100℃, then reduce to low heat at 70-80℃ and simmer for 0.5-1 hour, stirring occasionally. S4: Filter the decocted herbs through gauze to extract the liquid and dregs. Add an appropriate amount of water to the dregs again and repeat the above decoction and filtration steps. Combine the two liquids to obtain a Chinese herbal extract.

8. The long-acting dandelion and indigo compound according to claim 1, characterized in that, The components of the herbal extract, by weight, include: 250-300 parts dandelion, 220-280 parts isatis root, 150-180 parts indigo leaf, 120-150 parts honeysuckle, 50-100 parts andrographis paniculata, 50-80 parts houttuynia cordata, 150-200 parts astragalus, 80-120 parts scrophularia, 100-150 parts rehmannia root, 80-120 parts lysimachia christinae, 80-100 parts dictamnus dasycarpus root bark, 50-80 parts gentian root, and 80-100 parts anemarrhena rhizome.

9. The long-acting dandelion and indigo compound according to claim 1, characterized in that, The adjuvants are potassium sorbate, potassium iodide, β-cyclodextrin, and Tween-80.

10. The method for preparing the long-acting dandelion and indigo compound according to any one of claims 1-9, characterized in that, Includes the following steps: Step 1: Place the herbal extract in a container, control the temperature at 80-90℃, and concentrate the herbal extract to a concentration with a relative density of 1.2-1.5; Step 2: Add the adhesive microparticles and adjuvants to the concentrate in sequence and stir until homogeneous to obtain a mixture of traditional Chinese medicines; Step 3: Prepare different concentrations of the Chinese herbal medicine mixture with deionized water, and then sterilize it using pasteurization to obtain a long-acting dandelion and indigo Chinese herbal medicine compound.

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