Method for green phosphorus removal by targeting phosphorus accumulating organisms in water biological method and phosphorus immobilization carrier
Patent Information
- Application Number
- CN202611267256.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-08-20
- Publication Date
- 2026-10-20
AI Technical Summary
而清水生物法无活性污泥产生，系统中的磷无法通过“富磷污泥”外排而达到生物除磷的效果
[0032]1、传统活性污泥法硝化菌世代时间长，需要长泥龄（10～20d），保障脱氮效果；而聚磷菌通过排泥去除磷，聚磷菌世代时间短，需要短泥龄才能持续排出富磷污泥；本发明跳出传统活性污泥框架，无污泥龄的瓶颈。
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Abstract
Description
Technical Field
[0001] This invention belongs to the field of wastewater treatment technology, specifically relating to a green phosphorus removal method using a biological approach targeting polyphosphate-accumulating bacteria in synergy with a phosphorus-fixing carrier. Background Technology
[0002] Biological phosphorus removal currently relies on three core types of polyphosphate-accumulating bacteria, as follows: 1) Aerobic polyphosphate-accumulating bacteria (Ca. Accumulibacter), which are the core functional bacteria in traditional biological phosphorus removal systems. These bacteria rely on volatile fatty acids (VFAs) in the influent as a carbon source. Under anaerobic conditions, they absorb VFAs to synthesize intracellular poly-β-hydroxybutyrate (PHB) and release phosphate. In the aerobic stage, they use O2 as an electron acceptor to oxidize intracellular PHB for energy, and excessively absorb phosphate from the water and store it intracellularly, thus achieving aerobic phosphorus uptake. 2) Denitrifying phosphorus removal bacteria (Dechloromonas), which are typical denitrifying polyphosphate-accumulating bacteria. In the anaerobic stage, they utilize VFAs to store intracellular PHB as a carbon source. Under hypoxic conditions, they use nitrate as an electron acceptor to drive the oxidation of intracellular PHB, completing nitrate reduction and denitrification while simultaneously absorbing excessive phosphorus, achieving coupled denitrification and phosphorus removal metabolism, saving carbon source and aeration energy consumption. 3) Fermentation-based polyphosphate metabolism (Tetrasphaera) possesses the ability to ferment and produce acid from large organic molecules. It can directly degrade large substrates such as glucose and amino acids to generate VFA for its own polyphosphate metabolism, without relying on exogenous volatile fatty acids in the influent. This effectively compensates for the phosphorus removal shortcomings caused by low carbon sources and low VFA influent, resulting in stronger system resistance to shock loads and stable and efficient phosphorus removal performance. All three biological phosphorus removal methods rely on activated sludge processes. However, activated sludge only absorbs phosphorus essential for growth (1%-2% of cell dry weight), which is extremely low and cannot meet standards on its own. All three biological phosphorus removal methods require the discharge of activated sludge to enhance the biological phosphorus removal effect, and the advanced treatment unit still requires further chemical phosphorus removal to meet discharge standards.
[0003] High-efficiency biological phosphorus fixation and clear water biological methods complement each other. The core logic of clear water biological methods is to replace traditional activated sludge with targeted functional bacteria to achieve the goals of low carbon emissions, no sludge, and effluent meeting the standards for newly treated water. However, because phosphorus in wastewater cannot be completely degraded into gas by microorganisms (no gaseous phosphorus products), it can only be transferred from the water body to the sludge and discharged from the system through three pathways: speciation, microbial enrichment and sedimentation, and chemical precipitation. The so-called "biological phosphorus removal" is actually the transfer of phosphorus and solid-liquid separation. In contrast, clear water biological methods do not produce activated sludge, and phosphorus in the system cannot achieve the effect of biological phosphorus removal through the discharge of "phosphorus-rich sludge". In order to form a complete wastewater treatment technology system, high-efficiency biological phosphorus fixation has emerged.
[0004] Therefore, this invention proposes a green phosphorus removal method using a water-based biological approach targeting polyphosphate-accumulating bacteria in synergy with phosphorus-fixing carriers. Summary of the Invention
[0005] This invention aims to provide a green phosphorus removal method using a biological clean water process that targets polyphosphate-accumulating bacteria in synergy with a phosphorus-fixing carrier. This method involves introducing novel polyphosphate-accumulating bacteria and a highly efficient phosphorus-fixing carrier into an anaerobic tank, where the two work synergistically. The novel polyphosphate-accumulating bacteria attach specifically to the phosphorus-fixing carrier, efficiently accumulating phosphorus in a cyclical environment of anaerobic phosphorus release and aerobic phosphorus uptake. Simultaneously, the phosphorus-fixing carrier adsorbs and locks in phosphates, ultimately forming stable composite particles with high phosphorus content. These particles can be easily recycled and utilized as resources, completely solving the bottleneck of the biological clean water process's inability to discharge phosphorus-containing sludge, and achieving closed-loop management and value regeneration of phosphorus.
[0006] To achieve the above objectives, the technical solution of the present invention is as follows:
[0007] This invention provides a green phosphorus removal method using a water-based biological approach targeting polyphosphate-accumulating bacteria in conjunction with a phosphorus-fixing carrier, comprising the following steps:
[0008] S101. Add wastewater to the mixing tank, add solid polyphosphate bacteria agent, and mix thoroughly.
[0009] S102. Add the phosphorus fixation carrier, stir evenly, and obtain a mixture containing phosphorus fixation carrier containing polyphosphate-adsorbing bacteria.
[0010] S103. Pump the mixed liquor into the anaerobic tank of the clear water biological treatment system;
[0011] S104. A large amount of phosphorus is adsorbed by the carrier and settled in the sedimentation tank, and the sediment is discharged from the system periodically; some phosphorus-containing carrier-bacterial complex is discharged from the system periodically in the form of suspended solids.
[0012] The solid polyphosphate-accumulating agent is prepared by mixing seed culture of Candidatus Accumulibacter and Tetrasphaer at a volume ratio of 3:1; the mass ratio of the phosphorus-fixing carrier to the solid polyphosphate-accumulating agent is 1:0.8~1.5; and the daily pumping volume of the mixture is 0.015%~0.025% of the total influent.
[0013] Preferably, in step S101, the time for thorough mixing is not less than 0.5 hours, and the amount of wastewater added is not less than 2 / 3 of the volume of the mixing tank.
[0014] Preferably, the preparation method of the phosphorus fixation carrier includes: weighing zeolite, coal gangue and diatomaceous earth after high-temperature calcination and dry mixing to obtain a main material mixture, adding a modifier, and placing it in a sterile mixer for uniform stirring to obtain the phosphorus fixation carrier.
[0015] Preferably, the mass ratio of zeolite, coal gangue and diatomite is 10:18 to 22:1.
[0016] Preferably, the modifier is modified calcium carbonate powder and FeCl3 powder, and the amount of modified calcium carbonate powder and FeCl3 powder added is 0.15% to 0.25% of the mass of the main material.
[0017] Preferably, the time for uniform stirring is not less than 30 minutes.
[0018] Preferably, the preparation method of the solid polyphosphate inoculant includes the following steps:
[0019] S201, Preparation of Polyphosphate-accumulating Wet Mud
[0020] Seed cultures of Candidatus Accumulibacter and Tetrasphaer were mixed and subjected to graded activation and anaerobic-aerobic cycle enrichment and expansion to obtain polyphosphate-accumulating bacteria wet sludge.
[0021] S202, Polyphosphate-accumulating wet mud after protection
[0022] Add mannitol-maltodextrin composite protectant to polyphosphate-accumulating wet sludge, mix evenly, and obtain polyphosphate-accumulating wet sludge after protection.
[0023] S203, Preparation of Immobilized Polyphosphate-Accumulating Bacteria Solid Inoculum
[0024] Fresh polyphosphate-accumulating bacteria wet mud was slowly added to PVA-sodium alginate composite sol to obtain a bacterial-gel mixture. The bacterial-gel mixture was then dripped into a calcium chloride-boric acid composite crosslinking solution to form spherical particles. These particles were then completely immersed in the calcium chloride-boric acid composite crosslinking solution to solidify. The solidified particles were rinsed and dried to obtain solid polyphosphate-accumulating bacteria agent.
[0025] Preferably, the species in the Candidatus Accumulibacter genus is Candidatus Accumulibacter regalis, and the species in the Tetrasphaer genus are Tetrasphaera japonica, Tetrasphaera australiensi, and Tetrasphaerae longata, and the volume ratio of the seed liquid of Candidatus Accumulibacter regalis, Tetrasphaera japonica, Tetrasphaera australiensis, and Tetrasphaerae longata is 75:10:10:5.
[0026] Preferably, the step of carrying out graded activation and anaerobic-aerobic cycle enrichment and expansion includes: preparing a special culture medium with sodium acetate as the sole volatile fatty acid carbon source, inoculating the seed culture, and running it in stages at a constant temperature of 27°C; the anaerobic stage is carried out with closed stirring for 120 min; then aeration is turned on to enter the aerobic stage for 180 min, maintaining DO 1.5-2.0 mg / L; after a single cycle, the mixture is allowed to settle, and 70% of the supernatant is replaced with fresh culture medium, and this process is repeated for 7-10 cycles; after enrichment, the liquid is introduced into a fermenter for deep liquid expansion, and the anaerobic carbon storage and aerobic proliferation process are completed by segmented oxygen control. The fermentation broth is centrifuged and concentrated to obtain highly active polyphosphate-accumulating bacteria wet sludge.
[0027] Preferably, the mannitol-maltodextrin composite protectant is composed of mannitol and maltodextrin in a mass ratio of 1:2, and its addition amount is 2% to 3% of the mass of polyphosphate-rich wet sludge.
[0028] Preferably, the polyvinyl alcohol and sodium alginate in the polyvinyl alcohol-sodium alginate composite sol have mass fractions of 5% and 1%, respectively; the mass ratio of the polyvinyl alcohol-sodium alginate composite sol to the activated polyphosphate-rich wet sludge is 4:1; and the temperature of the polyvinyl alcohol-sodium alginate composite sol does not exceed 30°C.
[0029] Preferably, the calcium chloride-boric acid composite crosslinking solution is prepared by dissolving anhydrous calcium chloride in a saturated boric acid solution to achieve a mass fraction of 2% for the anhydrous calcium chloride, and the mass ratio of calcium chloride to polyphosphate-rich wet sludge is 2:22~28.
[0030] Preferably, the curing time is 30 minutes, the drying temperature does not exceed 40°C, the solid polyphosphate agent has a moisture content of 12%~15%, and a particle size of 3~5 mm.
[0031] Compared with existing technologies, the beneficial effects of this solution are:
[0032] 1. Traditional activated sludge processes have long generation times for nitrifying bacteria, requiring a long sludge age (10-20 days) to ensure nitrogen removal efficiency; while polyphosphate-accumulating bacteria remove phosphorus through sludge discharge, and their generation times are short, requiring a short sludge age to continuously discharge phosphorus-rich sludge; this invention breaks away from the traditional activated sludge framework, eliminating the bottleneck of sludge age.
[0033] 2. This invention provides targeted polyphosphate-accumulating bacteria for highly efficient phosphorus enrichment, while a phosphorus-fixing carrier simultaneously adsorbs and locks in phosphates, ultimately forming stable composite particles with high phosphorus content. Only the retention of suspended solids is needed to achieve biological phosphorus fixation and systemic phosphorus removal. Furthermore, the biological phosphorus fixation effect is highly efficient and stable; the total phosphorus in the effluent from biological phosphorus fixation alone meets discharge standards, eliminating the need for deep treatment or chemical phosphorus removal. The suspended solids generated by the phosphorus-fixing carrier adsorption are far less than the amount of inorganic sludge produced by chemical phosphorus removal. Attached Figure Description
[0034] Figure 1 This is a picture of a solid polyphosphate inoculant.
[0035] Figure 2 A physical image of a high-efficiency phosphorus fixation carrier;
[0036] Figure 3 Diagram of the Tianjin pilot plant;
[0037] Figure 4 This is an image showing the activation of solid polyphosphate-accumulating bacteria and high-efficiency phosphorus-fixing carriers on-site. Detailed Implementation
[0038] To facilitate understanding of the present invention by those skilled in the art, the technical solution of the present invention will be further described in detail below with reference to embodiments and accompanying drawings. Obviously, the described embodiments are merely some, not all, of the embodiments of the present invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort should fall within the scope of protection of the present invention.
[0039] Unless otherwise specified, the experimental methods used in the following examples are conventional methods.
[0040] Unless otherwise specified, all materials and reagents used in the following examples are commercially available.
[0041] Example 1
[0042] This embodiment provides a method for preparing targeted polyphosphate-accumulating bacteria, including the following steps:
[0043] 1. Preparation of wet polyphosphate-accumulating bacteria sludge
[0044] 1) Seed liquid compound
[0045] Seed cultures of Candidatus Accumulibacterregalis (type I branch) from the genus Candidatus Accumulibacter were mixed in the laboratory with seed cultures of Tetrasphaera japonica, Tetrasphaera australiensis, and Tetrasphaerae longata from the genus Tetrasphaer at a volume ratio of 75:10:10:5.
[0046] 2) Expansion Cultivation Steps
[0047] A phased activation and anaerobic-aerobic cycle enrichment and expansion culture were conducted. A specialized culture medium was prepared using sodium acetate as the sole volatile fatty acid carbon source. Seed culture from a biological laboratory was inoculated, and the culture was run in stages at a constant temperature of 27℃: An anaerobic stage was conducted with closed stirring for 120 minutes to maintain favorable anaerobic conditions and provide a reducing environment for polyphosphate-accumulating bacteria to absorb acetate and synthesize intracellular PHB; subsequently, aeration was initiated for 180 minutes in an aerobic stage, maintaining a DO concentration of 1.5–2.0 mg / L to encourage the bacteria to oxidize the internal carbon source using oxygen as an electron acceptor and to excessively absorb phosphorus. After each cycle, the culture was allowed to settle, and 70% of the supernatant was replaced with fresh culture medium. This process was repeated for 7–10 cycles. After enrichment, the liquid was transferred to a fermenter for deep liquid culture expansion, with segmented oxygen control to complete the anaerobic carbon storage and aerobic proliferation process. The fermentation broth was then centrifuged and concentrated to obtain highly active polyphosphate-accumulating bacteria wet sludge.
[0048] 2. Preparation of polyphosphate-accumulating agents
[0049] 2.1 Preparation of auxiliary materials
[0050] Calcium chloride-boric acid crosslinking curing liquid system: Dissolve anhydrous calcium chloride (CaCl2) in a saturated boric acid solution at room temperature to make the mass fraction of anhydrous calcium chloride 2%. The saturated boric acid solution refers to weighing 57g of boric acid and dissolving it in 1L of pure water.
[0051] Mannitol-maltodextrin composite protectant: Mannitol and maltodextrin are compounded in a mass ratio of 1:2 to obtain the product.
[0052] PVA-Sodium Alginate Composite Sol: Add polyvinyl alcohol and sodium alginate to sterile pure water at a ratio of 5:1 to make the mass fraction of polyvinyl alcohol 5%. Heat in a water bath and stir until completely transparent and dissolved, without particles or flocculent precipitates. Let stand to remove bubbles, and let cool naturally to a constant temperature of 30°C for later use. Strictly control the temperature to avoid high temperature inactivation of bacteria.
[0053] 2.2 Preparation of polyphosphate-accumulating agents
[0054] The preparation method of immobilized solid polyphosphate-accumulating bacteria includes the following steps:
[0055] 1) Add 2%~3% (by weight) of mannitol-maltodextrin composite protectant to the wet polyphosphate-accumulating bacteria sludge, mix thoroughly, and obtain fresh, protected wet polyphosphate-accumulating bacteria sludge. This step provides dual protection against bacterial cell oxidation and damage to intracellular PHB and polyphosphate particles during the subsequent 40℃ low-temperature drying and gel cross-linking processes, maximizing the preservation of polyphosphate-accumulating bacteria's anaerobic phosphorus release and aerobic superphosphate uptake activities.
[0056] 2) Add the fresh polyphosphate bacteria wet mud after protection to the 30℃ constant temperature PVA-sodium alginate composite sol at a mass ratio of 1:4. Stir gently at low speed to make the bacteria evenly dispersed in the gel system, ensuring no bacterial agglomeration and no local precipitation, and obtain the bacterial-gel mixture.
[0057] 3) Use a sterile syringe to draw up the bacterial adhesive mixture at a uniform speed and drop it vertically and at a uniform speed into the calcium chloride-boric acid composite crosslinking solution, so that the mass ratio of calcium chloride to polyphosphate bacteria wet mud is 2:25; the droplets ion crosslink and form spontaneously regular spherical particles upon contact with the crosslinking solution.
[0058] 4) The molded particles are completely immersed in the crosslinking solution and allowed to stand at room temperature for 30 minutes to solidify the gel network structure and prevent the bacteria from falling off or disintegrating during subsequent use.
[0059] 5) Remove the solidified particles and rinse them thoroughly with sterile pure water multiple times to completely remove residual calcium chloride and boric acid from the particle surface, so as to avoid residual salt affecting the activity of bacteria and the water treatment effect.
[0060] 6) Spread the rinsed granules evenly on the drying tray and dry them with low-temperature hot air at 40℃. The temperature must be strictly controlled not to exceed 40℃ throughout the process. Dry until the moisture content of the granules is stable at 12% to 15%. Do not over-dry, as this may cause the bacteria to become inactive or the granules to crack.
[0061] 7) Select regular particles with a particle size of 3-5mm, round shape, dense structure, no cracks, and no whitening or powdering. These are the finished polyphosphate bacteria solid inoculant.
[0062] Example 2
[0063] This embodiment provides a method for preparing a high-efficiency phosphorus fixation carrier, including the following steps:
[0064] 1) Purchase zeolite, coal gangue, and diatomaceous earth that have been activated by high-temperature roasting on the market, crush them separately and pass them through 80 mesh, and dry mix them in a ratio of 10:20:1 to obtain the total material.
[0065] 2) Add modified calcium carbonate powder and powdered FeCl3 (both accounting for 0.2% of the total material mass) separately, place them in a sterile mixer and stir at a constant speed for 30 minutes to ensure that the modifier is uniformly loaded on the mineral pores and surface, and finally obtain a highly efficient phosphorus fixation carrier with well-developed pores, abundant calcium active sites and iron elements attached to the surface.
[0066] This phosphorus fixation carrier has the following functions: physical adsorption of phosphate, providing attachment sites for polyphosphate-accumulating bacteria, and promoting intracellular polyphosphate synthesis with trace amounts of calcium and iron.
[0067] Example 3
[0068] This embodiment provides a green phosphorus removal method using a water-based biological approach targeting polyphosphate-accumulating bacteria in conjunction with a phosphorus fixation carrier, comprising the following steps:
[0069] 1) Add sewage to the activation mixing tank until it is 2 / 3 full (the volume of the activation tank depends on the daily inflow of the target sewage treatment plant's biological treatment tank, generally 0.01%), add polyphosphate-accumulating bacteria solid inoculant, and stir thoroughly for 0.5 hours.
[0070] 2) After the solid polyphosphate-accumulating bacteria agent is fully dissolved and stirred evenly, add an efficient phosphorus-fixing carrier of the same mass as the solid polyphosphate-accumulating bacteria agent, so that the polyphosphate-accumulating bacteria can be adsorbed and enriched inside the microporous material to obtain a mixture of agent and carrier.
[0071] 3) Pump the mixed liquor into the anaerobic tank. Pump in 0.01% of the total influent volume of the mixed liquor daily. Polyphosphate-accumulating bacteria multiply on the phosphorus fixation carrier and continuously take in phosphorus in excess of physiological needs, which exists in the cells in the form of polyphosphate. The phosphorus fixation carrier simultaneously adsorbs and locks in phosphate, eventually forming stable composite particles with high phosphorus content, thus achieving the effect of highly efficient biological polyphosphate fixation.
[0072] It should be noted that the volume of the mixing tank is 1-2 cubic meters. If the daily water inflow is 10,000 cubic meters, the dosage of polyphosphate-accumulating bacteria solid agent is 1 ton. However, it can be added in batches. The specific dosage depends on the site conditions and varies.
[0073] Example 4
[0074] This embodiment was carried out at the pilot-scale test site of the clean water biological treatment plant in the Tianjin Binhai New Area Eco-City Wastewater Treatment Plant. The pilot-scale unit has a treatment capacity of 75m³. 3 The process follows a "filtration-anaerobic-aerobic 1-anoxic-aerobic 2" flow, with the biological reactor (also known as the anaerobic, anoxic, and aerobic reactors) serving as a collective term. The total hydraulic retention time (HRT) of the biological reactor is 21.6 hours, and the mixed liquor suspended solids (MLSS) is controlled between 80 and 100 mg / L. The operating conditions within the biological reactor are: pH 7.5 ± 0.3 and temperature 25 ± 2°C.
[0075] Based on a calculation of 0.01% of the total influent volume, approximately 7 kg each of the phosphorus-fixing carrier prepared in Example 2 and the polyphosphate-accumulating bacteria agent prepared in Example 1 are added daily, uniformly at the inlet of the aerobic tank. No operating parameters within the tank are adjusted after addition.
[0076] Specific methods:
[0077] 1) The phosphorus fixation carrier and polyphosphate-accumulating bacteria agent are mixed at a mass ratio of 1:1 and a mixed solution of highly efficient phosphorus fixation carrier for adsorbing and enriching polyphosphate-accumulating bacteria is prepared in a stirred tank.
[0078] 2) The mixed liquor is evenly added to the clear water biological treatment system at the anaerobic end of the biological treatment tank. The daily amount of mixed liquor added is one ten-thousandth of the total influent volume of the system.
[0079] 3) Polyphosphate-accumulating bacteria naturally colonize the carrier surface under the conditions of the clear water biological method pond. Since the nutritional conditions of polyphosphate-accumulating bacteria are aligned with those of heterotrophic bacteria in the clear water biological method, including C / N ratio (5:1~8:1), pH range (7~8), dissolved oxygen range (2~4mg / L) and temperature range (25~30°C) during the aerobic process, polyphosphate-accumulating bacteria can survive and reproduce in the pond without additional condition adjustments and form a biomass enhancement layer on the activated carbon surface.
[0080] 4) Polyphosphate-accumulating bacteria multiply on the phosphorus-fixing carrier, providing additional adsorption sites and continuously adsorbing excessive amounts of phosphorus, thus enhancing the phosphorus-fixing capacity of the system. Finally, the carrier-bacterial complex containing a large amount of phosphorus settles and is discharged in the sedimentation tank. The frequency is determined according to the total phosphorus concentration of the influent and the total phosphorus requirements of the effluent, usually once every 1 to 3 days.
[0081] 5) In addition, some phosphorus-containing carrier-bacterial complexes are periodically discharged from the system as suspended solids, and the total phosphorus in the filtered effluent is monitored daily. The final effluent total phosphorus is consistently below 0.3 mg / L, representing a phosphorus treatment efficiency improvement of no less than 30% compared to the original tank. Phosphorus-containing suspended solids are mixed with the effluent and discharged daily, and the system MLSS is maintained below 100 mg / L.
[0082] The above specific embodiments are merely explanations of the present invention and are not intended to limit the present invention. After reading this specification, those skilled in the art can make modifications to these embodiments without contributing any inventive step, but as long as they are within the scope of the claims of the present invention, they are protected by patent law.
Claims
1. A green phosphorus removal method using a water-based biological approach targeting polyphosphate-accumulating bacteria in synergy with a phosphorus-fixing carrier, characterized in that: Includes the following steps: S101. Add wastewater to the mixing tank, add solid polyphosphate bacteria agent, and mix thoroughly. S102. Add the phosphorus fixation carrier, stir evenly, and obtain a mixture containing phosphorus fixation carrier containing polyphosphate-adsorbing bacteria. S103. Pump the mixed liquor into the anaerobic tank of the clear water biological treatment system; S104. A large amount of phosphorus is adsorbed by the carrier and settled in the sedimentation tank, and the sediment is discharged from the system periodically; some phosphorus-containing carrier-bacterial complex is discharged from the system periodically in the form of suspended solids. The solid polyphosphate-accumulating agent is prepared by mixing seed culture of Candidatus Accumulibacter and Tetrasphaer at a volume ratio of 3:1; the mass ratio of the phosphorus-fixing carrier to the solid polyphosphate-accumulating agent is 1:0.8~1.5; and the daily pumping volume of the mixture is 0.015%~0.025% of the total influent.
2. The method for green phosphorus removal using a water-based biological targeted polyphosphate-accumulating bacteria synergistically with a phosphorus fixation carrier, as described in claim 1, is characterized in that... In step S101, the time for thorough mixing is not less than 0.5 hours, and the amount of wastewater added is not less than 2 / 3 of the volume of the mixing tank.
3. The method for green phosphorus removal using a water-based biological targeted polyphosphate-accumulating bacteria synergistically with a phosphorus fixation carrier, as described in claim 1, is characterized in that... The method for preparing the phosphorus fixation carrier includes: weighing zeolite, coal gangue and diatomaceous earth after high-temperature calcination and dry mixing to obtain a main material mixture, adding a modifier, and then placing it in a sterile mixer for uniform stirring to obtain the phosphorus fixation carrier.
4. The method for green phosphorus removal using a water-based biological targeted polyphosphate-accumulating bacteria synergistically with a phosphorus fixation carrier, as described in claim 3, is characterized in that... The mass ratio of zeolite, coal gangue, and diatomite is 10:18 to 22:
1.
5. The method for green phosphorus removal using a water-based biological targeted polyphosphate-accumulating bacteria synergistically with a phosphorus-fixing carrier, as described in claim 3, is characterized in that... The modifier is modified calcium carbonate powder and FeCl3 powder, and the amount of modified calcium carbonate powder and FeCl3 powder added is 0.15% to 0.25% of the mass of the main material.
6. The method for green phosphorus removal using a water-based biological targeted polyphosphate-accumulating bacteria synergistically with a phosphorus fixation carrier, as described in claim 3, is characterized in that... The time for uniform stirring shall not be less than 30 minutes.
7. The method for green phosphorus removal using a water-based biological targeted polyphosphate-accumulating bacteria synergistically with a phosphorus fixation carrier, as described in claim 1, is characterized in that... The preparation method of the solid polyphosphate inoculant includes the following steps: S201, Preparation of Polyphosphate-accumulating Wet Mud Seed cultures of Candidatus Accumulibacter and Tetrasphaer were mixed and subjected to graded activation and anaerobic-aerobic cycle enrichment and expansion to obtain polyphosphate-accumulating bacteria wet sludge. S202, Polyphosphate-accumulating wet mud after protection Add mannitol-maltodextrin composite protectant to polyphosphate-accumulating wet sludge, mix evenly, and obtain polyphosphate-accumulating wet sludge after protection. S203, Preparation of Immobilized Polyphosphate-Accumulating Bacteria Solid Inoculum Fresh polyphosphate-accumulating bacteria wet mud was slowly added to PVA-sodium alginate composite sol to obtain a bacterial-gel mixture. The bacterial-gel mixture was then dripped into a calcium chloride-boric acid composite crosslinking solution to form spherical particles. These particles were then completely immersed in the calcium chloride-boric acid composite crosslinking solution to solidify. The solidified particles were rinsed and dried to obtain solid polyphosphate-accumulating bacteria agent.
8. The method for green phosphorus removal using a water-based biological targeted polyphosphate-accumulating bacteria synergistically with a phosphorus fixation carrier, as described in claim 7, is characterized in that... The species in the Candidatus Accumulibacter genus is Candidatus Accumulibacterregalis, and the species in the Tetrasphaer genus are Tetrasphaera japonica, Tetrasphaera australiensi, and Tetrasphaerae longata. The volume ratio of the seed liquid of Candidatus Accumulibacter regalis, Tetrasphaera japonica, Tetrasphaera australiensis, and Tetrasphaerae longata is 75:10:10:
5.
9. The method for green phosphorus removal using a water-based biological targeted polyphosphate-accumulating bacteria synergistically with a phosphorus fixation carrier, as described in claim 7, is characterized in that... The process of graded activation and anaerobic-aerobic cycle enrichment and expansion includes: preparing a special culture medium using sodium acetate as the sole volatile fatty acid carbon source, inoculating the seed culture, and operating in stages at a constant temperature of 27℃; the anaerobic stage is carried out with closed stirring for 120 min; then aeration is turned on to enter the aerobic stage for 180 min, maintaining DO at 1.5-2.0 mg / L; after a single cycle, the mixture is allowed to settle, and 70% of the supernatant is replaced with fresh culture medium, and this process is repeated for 7-10 cycles; after enrichment, the liquid is transferred to a fermenter for deep liquid expansion, and the anaerobic carbon storage and aerobic proliferation processes are completed by segmented oxygen control. The fermentation broth is then centrifuged and concentrated to obtain highly active polyphosphate-accumulating bacteria wet sludge.
10. The method for green phosphorus removal using a water-based biological targeted polyphosphate-accumulating bacteria synergistically with a phosphorus fixation carrier, as described in claim 7, is characterized in that... The mannitol-maltodextrin composite protective agent is composed of mannitol and maltodextrin in a mass ratio of 1:2, and its addition amount is 2% to 3% of the mass of polyphosphate-rich wet sludge.
11. The method for green phosphorus removal using a water-based biological targeted polyphosphate-accumulating bacteria synergistically with a phosphorus fixation carrier, as described in claim 7, is characterized in that... The polyvinyl alcohol-sodium alginate composite sol contains 5% polyvinyl alcohol and 1% sodium alginate by mass, respectively; the mass ratio of the polyvinyl alcohol-sodium alginate composite sol to the activated polyphosphate-rich wet sludge is 4:1; and the temperature of the polyvinyl alcohol-sodium alginate composite sol does not exceed 30°C.
12. The method for green phosphorus removal using a water-based biological targeted polyphosphate-accumulating bacteria synergistically with a phosphorus fixation carrier, as described in claim 7, is characterized in that... The calcium chloride-boric acid composite crosslinking solution is prepared by dissolving anhydrous calcium chloride in a saturated boric acid solution to achieve a mass fraction of 2% for the anhydrous calcium chloride, and the mass ratio of calcium chloride to polyphosphate-rich wet sludge is 2:22~28.
13. The method for green phosphorus removal using a water-based biological targeted polyphosphate-accumulating bacteria synergistically with a phosphorus fixation carrier, as described in claim 7, is characterized in that... The curing time is 30 minutes, the drying temperature does not exceed 40°C, the solid polyphosphate agent has a moisture content of 12%~15% and a particle size of 3~5 mm.