Far infrared oxygen water having immune and active oxygen inhibiting effects and a method of preparing the same

Abstract

This invention discloses a far-infrared oxygenated water and its preparation method. The method first sorts natural minerals with a far-infrared emissivity of 0.8 or higher, such as tourmaline and jade, and pulverizes them. Then, it utilizes an energy injection O2 water system, where pressure brings the minerals into contact with the water, transferring far-infrared energy to the water. Next, medical-grade high-purity oxygen is injected in nano-sized microbubbles. The pH value is adjusted to 8.0-9.5 using natural alkaline minerals. After composite filtration and multi-stage sterilization, it is finally sealed and packaged in a nitrogen-filled environment. The resulting far-infrared oxygenated water has a far-infrared emissivity of 0.869, an oxygen content of 80-120 ppm, a pH of 8.5, and a mineral TDS of 189. It possesses multiple health benefits, including enhancing immunity and inhibiting reactive oxygen species. It has passed HACCP certification and FDA approval, and special packaging ensures the stability of the active ingredients.

Description

Technical Field

[0001] This invention relates to a functional drinking water product that utilizes far-infrared energy to deliver energy into water and its preparation method, and more particularly to a far-infrared oxygenated water product that enhances immunity and inhibits reactive oxygen species and its preparation method. Background Technology

[0002] In modern society, environmental pollution, life stress, and irregular lifestyles lead to a decline in people's immunity and a large accumulation of reactive oxygen species (ROS) in the body. Immunity is the body's core defense mechanism against external pathogens; once weakened, various diseases can easily take hold. Excessive ROS not only damage cells and accelerate the aging process, but are also a significant factor in inducing many chronic diseases.

[0003] To address these issues, numerous functional beverages and health foods have emerged on the market. However, most products simply add specific nutrients or functional ingredients, while methods to enhance biological activity by altering the structure and properties of water itself are relatively scarce.

[0004] The current functional drinking water market offers a wide variety of products, such as alkaline ionized water, mineral water, and oxygenated water. Alkaline ionized water is produced through electrolysis and can adjust pH levels, but its oxygen content is limited and it lacks far-infrared energy. Mineral water contains minerals such as calcium and magnesium, which exist mostly in a simple dissolved state, resulting in low bioavailability. The oxygen injected into acidic water is easily dissipated and difficult to preserve for long periods, making its effects short-lived.

[0005] Far-infrared rays, as a type of long-wave electromagnetic wave, can penetrate deep into the human body, activating the vibration and rotation of water molecules and reducing water molecule clusters. This characteristic can enhance cell membrane permeability, improve water metabolism, and promote the excretion of waste products from the body. However, traditional far-infrared water treatment methods often involve immersion or simple contact, resulting in low energy transfer efficiency and unsatisfactory actual effects.

[0006] Oxygen is essential for cellular energy production and maintaining immune system function, and increasing oxygen supply can bring many physiological benefits. However, dissolved oxygen in ordinary water is only 8-10 ppm, and current oxygenated water production technologies struggle to consistently maintain high concentrations of dissolved oxygen.

[0007] Therefore, there is an urgent need for a functional drinking water manufacturing technology that can organically combine far-infrared energy transmission, high-concentration dissolved oxygen, and alkaline pH regulation to enhance immunity and inhibit free radicals. This requires innovative methods to achieve efficient transfer of far-infrared energy from natural minerals, as well as overcoming the technical bottlenecks of stable oxygen dissolution in microbubbles and alkalinity regulation by natural minerals. Simultaneously, developing filtration and sterilization technologies that balance microbial safety and long-term preservation, as well as packaging technologies that reduce oxygen loss and maintain far-infrared energy, are also crucial issues that urgently need to be addressed.

[0008] Based on the above technical background, this invention proposes a method for preparing far-infrared oxygenated water. This method utilizes an EIOW (Energy Injection into O2 Water) system to efficiently transfer high far-infrared emissivity mineral energy into water, stably dissolving microbubble oxygen. Furthermore, by adjusting the alkaline pH value, the product is endowed with the effects of enhancing immunity and inhibiting reactive oxygen species.

[0009] Previous documents

[0010] [Patent Documents]

[0011] (Patent Document 1) Korean Patent 10-2009-0052669

[0012] (Patent Document 2) Korean Publication Patent 10-2011-0034122

[0013] (Patent Document 3) Korean Patent 10-0664599

[0014] (Patent Document 4) Korean Patent 10-0620067 Summary of the Invention

[0015] This invention focuses on developing a technology to efficiently transfer far-infrared energy emitted by natural minerals into water. This energy transfer reduces the size of water molecule clusters, enhances the biological activity of water, and lays the foundation for optimizing the performance of functional drinking water.

[0016] In terms of oxygen dissolution technology, this invention focuses on developing a new method to stably dissolve oxygen in water in the form of microbubbles, thereby maintaining a high concentration of oxygen. Given the drawback of ordinary oxygenated water where oxygen easily dissipates rapidly over time, this invention utilizes nanoscale microbubble technology combined with optimized pressure conditions to effectively improve oxygen solubility, ensuring its stability over a longer period and overcoming the technical bottlenecks of traditional oxygenated water.

[0017] Regarding pH regulation, this invention focuses on stabilizing far-infrared energy and oxygen-enriched water within a beneficial alkaline range (pH 8.0-9.5). Unlike alkaline ionized water produced by electrolysis, this invention utilizes alkaline minerals extracted from natural minerals to achieve natural pH regulation, providing the body with functional drinking water that neutralizes internal acidification and better meets health needs.

[0018] Furthermore, this invention features a meticulously designed filtration and sterilization process. This process fully preserves the three key characteristics of far-infrared energy, high oxygen concentration, and suitable alkalinity while ensuring microbial safety and long-term product preservation. By constructing a complex filtration system and combining it with a multi-stage sterilization process, the final product is ensured to be both safe and reliable, as well as fresh and long-lasting.

[0019] The present invention solves the technical problem by adopting the following technical solution:

[0020] In the method for preparing far-infrared oxygenated water with the effects of enhancing immunity and inhibiting reactive oxygen species, the present invention selects a mineral with high far-infrared emissivity, crushes it, contacts it with water under pressure to transfer far-infrared energy, injects microbubble oxygen and controls it to become alkaline, and then packages it after filtration, sterilization and treatment. Detailed Implementation

[0021] At this point, (a) minerals with a far-infrared emissivity of 0.8 or higher are screened; (b) the selected minerals are crushed and prepared into granular form; (c) under pressure, far-infrared energy is transferred to water by contacting the granular minerals with water; (d) oxygen in the form of microbubbles is injected into the water in which far-infrared energy has been transferred; (e) the pH of the oxygenated water is controlled within a range of not less than 8.0 but not more than 9.5; (f) the pH-controlled water is filtered; (g) the filtered water is disinfected; and (h) the disinfected water is packaged.

[0022] At this point, (a) involves preparing natural minerals such as tourmaline, iris, illite, germanium, and jade, measuring their emissivity with a far-infrared radiation tester, selecting minerals with high emissivity and a certain wavelength range, and then activating them by heat treatment to improve their far-infrared emissivity. (b) involves gradually coarsely grinding, regrinding, and finely grinding the selected minerals into fine particles, and then separating the particles with uniform particle size distribution using a sieve. The final particles with low moisture content are prepared by washing, stirring, and drying with pure water. (c) involves placing the mineral particles and pure water into a pressure vessel of an O2-injected water system, maintaining a certain temperature under pressure, and vibrating with an ultrasonic vibration device to improve the efficiency of far-infrared energy transfer. The process is verified by infrared spectroscopy and cooled by a heat exchanger. (d) involves converting medical-grade high-purity oxygen into nano-sized microbubbles using a microbubble generator, injecting the cooled far-infrared energy into the transferred water under pressure, stirring with a magnetic stirrer to increase the solubility of oxygen, and repeating this process until the target content is reached as measured by a dissolved oxygen meter. (e) involves extracting carbonic acid from natural minerals. A mixture of alkaline minerals in the form of calcium and magnesium chloride is added to oxygenated water and stirred. The pH is then measured with a digital pH meter, and the amount of minerals added is adjusted to reach the alkaline range. The mixture is stabilized at low temperature to check whether pH and conductivity fluctuations are minimized. Step (f) involves a pretreatment filter to remove coarse particles, an intermediate filter to remove fine particles, and a composite filter consisting of a precision filter and an activated carbon filter to adjust the mineral balance. Turbidity and conductivity are measured. If the values ​​do not meet the standards, the filtration process is repeated. Step (g) involves preheating the filtered water, then performing primary sterilization with an ultraviolet sterilizer, injecting medical ozone for secondary sterilization, removing residual microorganisms through a silver nanofilter, and performing microbial testing with a bacterial detection system. If the values ​​do not meet the standards, the sterilization process is repeated. Step (h) involves checking the residual oxygen in the sterilized water, transferring it to a clean room filled with nitrogen, filling it into a pre-sterilized container, injecting high-purity nitrogen into the top space, sealing it with a special cap, displaying manufacturing information, and storing only suitable products at low temperature after quality checks.

[0023] At this point, step (a) is: (a1) preparing natural minerals, including tourmaline, sapphire, illite, germanium, and jade; (a2) measuring the emissivity of the prepared natural minerals using a far-infrared radiation meter within a temperature range of 37°C to 40°C; and (a3) ​​screening for minerals with an emissivity of 0.8 or higher and a radiation energy of 350 W / m within a wavelength range of 8 to 14 μm. 2 • Natural minerals with a diameter of μm or higher; (a4) the step of heat-treating the selected natural minerals at a temperature of 600°C to 850°C for 3 to 24 hours to activate them, and the step of confirming that the far-infrared emissivity after heat treatment is increased by more than 10% compared with that before treatment.

[0024] At this point, step (b) is as follows: (b1) coarsely grind the selected minerals to a size of 5 mm or smaller using a primary grinder; (b2) re-crush the coarsely crushed minerals to a size of 1 mm or smaller using a secondary grinder; (b3) finely grind the re-crushed minerals to a size of 200 mesh or smaller (74 micrometers or smaller) using a fine grinder; (b4) uniformly sort the particle size distribution using a bulk separator, with a standard deviation of particle size within ±10%; (b5) prepare final particles with a moisture content of 1.0% or less by washing the selected mineral particles with pure water, but using pure water equivalent to 5 times the weight of the minerals, stirring at a speed of 150 rpm or higher for 10 to 30 minutes, and then drying at a temperature of 100°C to 120°C for at least 2 hours and no more than 5 hours.

[0025] At this point, step (c) is as follows: (c1) The mineral particles are introduced into the pressure vessel of the Energy Injection O2 Water (EIOW) system at a rate of not less than 50g and not more than 100g per liter of water. This system transfers far-infrared radiating minerals and far-infrared energy into the water through contact; (c2) Pure water is injected into the pressure vessel, and a pressure of not less than 3 atmospheres and not more than 10 atmospheres is applied; (c3) Under the above pressure, the temperature is maintained at a temperature exceeding 30°C and not more than 50°C for more than 10 minutes and not more than 60 minutes to allow for conversion through resonant absorption by water molecules. (c4) Simultaneously with step (c3), the interior of the pressure vessel is vibrated at a frequency exceeding 20 kHz to 40 kHz using an ultrasonic vibration device. Compared with the micro-vibration measured by infrared spectroscopy, the transmission efficiency of far-infrared energy is improved by more than 20%, and the water transfer of far-infrared energy is verified by the absorption spectrum change in the range of 3000-4000 cm⁻¹ using an infrared spectrophotometer; (c5) The water transferred from the far-infrared energy is cooled by a heat exchanger at a rate exceeding 5°C to 45°C and at a rate exceeding 5°C to 10°C.

[0026] At this point, step (d) is as follows: (d1) Prepare oxygen with a medical purity of 99.5% or higher; (d2) Convert the oxygen into microbubbles with a diameter greater than 30 nm and less than 100 nm using a microbubble generator; (d3) Inject the oxygen in the form of microbubbles into water cooled to above 5°C and below 15°C, and deliver far-infrared energy into the water at a pressure greater than 1.5 atmospheres and not exceeding 3 atmospheres; (d4) Stir the oxygenated water in the form of microbubbles at a speed greater than 350 rpm and not exceeding 500 rpm for more than 20 minutes and not exceeding 40 minutes using a magnetic stirrer to improve oxygen solubility; (d5) After stirring, measure the oxygen content using a dissolved oxygen meter, and repeat the oxygen injection process in steps (d3) and (d4) until it reaches a range of 80 ppm or greater and not exceeding 120 ppm.

[0027] [Comparative Example 1]

[0028] This comparative example concerns ordinary pure water that has not undergone special treatment, such as far-infrared radiation materials or oxygen injection, and is prepared through the following steps.

[0029] (Basic Purified Water Preparation) Tap water was sequentially passed through an activated carbon filter, ion exchange resin, and a reverse osmosis (RO) system. After the purification process, the conductivity of the water was measured to be 4.5 μS / cm, and the total dissolved solids (TDS) was measured to be 3 mg / L. The pH of the purified water was 6.8 without any adjustment, and the dissolved oxygen content was 8.3 ppm.

[0030] The purified water was simply filtered through a single 5μm pore size filter. The turbidity after filtration was measured to be 0.2 NTU.

[0031] The sterile purified water is filled into PET containers in a standard workshop without special environmental conditions. Approximately 5mm of headroom is left inside the containers, which are then sealed with ordinary plastic caps; nitrogen injection is not required. The product is labeled with its production date and expiration date and should be stored at room temperature (25°C).

[0032] [Comparative Example 2] Simple Far-Infrared Mineral Immersion Index

[0033] This comparative example is water prepared by simply soaking far-infrared emitting minerals without pressure or ultrasonic vibration, through the following steps.

[0034] (Mineral sorting and processing) The implementation method was the same as in Example 1, and natural minerals including tourmaline, neem, illite, germanium, and jade were prepared. The far-infrared emissivity of these minerals was measured to be 0.91, and the emissivity was increased to 0.95 after heat treatment at 700°C for 12 hours.

[0035] The implementation method is the same as in Example 1, except that the mineral is crushed and purified into fine particles with an average particle size of 50 μm, washed and dried with pure water.

[0036] The prepared mineral particles were added to a glass container at a rate of 75 g per 1 L of pure water and simply soaked at room temperature (25°C) for 72 hours without pressure or ultrasonic vibration. After soaking, the minerals were removed by filtration. As measured by an infrared spectrophotometer, only a slight change was observed in the OH stretching band of water molecules, which was about 80% lower than that observed in Example 1 using the EIOW system.

[0037] The water treated by immersion was then passed through a pretreatment filter with a pore size of 5 μm and an intermediate filter with a pore size of 1 μm for a simple filtration process. The turbidity of the filtered water was measured to be 0.7 NTU.

[0038] [Comparative Example 3] Oxygenated water (without far-infrared treatment)

[0039] This comparative example concerns oxygenated water prepared using only a high concentration of oxygen without the use of far-infrared emitting materials, through the following steps.

[0040] (Basic Purified Water Preparation) The method is the same as in Example 1. Tap water is passed through an activated carbon filter, ion exchange resin, and a reverse osmosis (RO) system to prepare purified water. After the purification process, the conductivity of the water was measured to be 4.8 μS / cm, and the total dissolved solids (TDS) was 3.2 mg / L. The measured pH of the purified water was 6.9, and the dissolved oxygen content was 8.4 ppm.

[0041] (Microbubble Oxygen Injection) The method is the same as above. Medical-grade oxygen with a purity of 99.8% is converted into microbubbles with an average diameter of 50 nm using a microbubble generator. This microbubble oxygen is injected into pure water at 15°C and cooled to 2°C under a pressure of 2 atmospheres. The oxygenated water is stirred at 450 rpm for 30 minutes using a magnetic stirrer to improve oxygen solubility. After measurement using a dissolved oxygen meter, the oxygen content is confirmed to reach 100 ppm.

[0042] (Filtration treatment) was performed using the same multi-stage filtration system as in Example 1 (a combination of a 7 μm pretreatment filter, a 2 μm intermediate filter, a 0.3 μm fine filter, and an activated carbon filter). However, a mineral-impregnated ceramic filter was not used. The turbidity of the filtered water was measured at 0.25 NTU, and the conductivity was 5.2 μS / cm at 25°C.

[0043] (Sterilization Treatment) The example is the same as Example 1, with sterilization performed in three stages (ultraviolet light, ozone, and nano-silver filter). The sterilization effect was checked, and the total aerobic bacteria count was found to be less than 5 CFU / ml, with no Escherichia coli or Staphylococcus detected.

[0044] Sterilized oxygenated water was filled into PET containers in a standard workshop. The top clearance inside the containers was approximately 10 mm, but they were sealed with ordinary plastic caps without the need for nitrogen injection. The product was labeled with the manufacturing date and expiration date and stored at 15°C. After 24 hours, the residual oxygen level was measured at 83% of the initial measurement, indicating rapid oxygen loss without nitrogen injection.

[0045] [Comparative Example 4] Alkaline water (without far-infrared radiation and oxygen treatment)

[0046] This comparative example concerns alkaline water prepared by adding only alkaline minerals without far-infrared radiation or oxygen injection, and it is prepared through the following steps.

[0047] (Basic Purified Water Preparation) The method is the same as in Example 1. Tap water is passed through an activated carbon filter, ion exchange resin, and a reverse osmosis (RO) system to prepare purified water. After the purification process, the conductivity of the water was measured to be 4.5 μS / cm, and the total dissolved solids (TDS) was 3.1 mg / L. The measured pH of the purified water was 6.8, and the dissolved oxygen content was 8.3 ppm.

[0048] (pH Control and Mineral Addition) Example 1: The same natural alkaline mineral mixture (calcium carbonate and magnesium chloride, weight ratio 60:40) was added to purified water at a concentration of 3 g / L and stirred at 200 rpm for 20 minutes at 27°C. The pH value measured using a digital pH meter was 8.5. The pH was stabilized at 7°C for 36 hours to confirm the stability of the controlled pH value. After stabilization, the pH fluctuation was within ±0.2, and the conductivity remained within ±4% of the initial measurement value.

[0049] (Filtration treatment) The same multi-stage filtration system as in Example 1 was used. However, a mineral-impregnated ceramic filter was not used. The turbidity of the filtered water was measured at 0.35 NTU, and the conductivity was 205 μS / cm at 25°C.

[0050] In a standard workshop, sterile alkaline water is filled into PET containers. Approximately 8mm of headroom is left inside the containers, which are then sealed with standard plastic caps; nitrogen injection is not required. The product is labeled with its production date and expiration date and should be stored at 15°C.

[0051] [Evaluation of immune-enhancing effects]

[0052] This experiment was conducted on companion animals to evaluate the immune-enhancing effects of far-infrared oxygenated water. The pets used in the experiment were 42 healthy beagles (21 females and 21 males), aged 12–18 months and weighing 10–15 kg. All animals underwent a 4-week acclimatization period before the experiment and were fed the same diet. This experiment was approved by the Animal Ethics Committee (Approval No.: AEC-2024-035).

[0053] The experimental animals were randomly divided into 7 groups (6 animals in each group, 3 males and 3 females): control group (normal purified water), Example 1 (far-infrared oxygenated water), comparative example 1 (normal purified water), comparative example 2 (simple far-infrared mineral immersion index), comparative example 3 (oxygenated water), and comparative example 4 (alkaline water).

[0054] The sample was administered orally to each group of experimental animals at a dose of 10 ml per kilogram of body weight, twice daily (9:00 AM and 5:00 PM) for 4 weeks (28 days). Blood samples were collected from each animal at the start of the experiment (day 0), day 14, and day 28, and immune-related indicators were analyzed.

[0055] Measure the following indicators to assess immunity.

[0056] 1. White blood cell count (WBC) and lymphocyte ratio: Measured using an automated blood cell analyzer.

[0057] 2. NK cell activity: Monocytes (PBMCs) isolated from peripheral blood were used, and the activity of CD3-CD56+ cells (NK cells) was measured by flow cytometry. NK cell activity was evaluated by cytotoxicity against target cells (K562 cell line), with a target cell to affected cell ratio of 1:10.

[0058] 3. Lymphocyte proliferation capacity: Lymphocytes isolated from peripheral blood were stimulated with konnabalin A (5 μg / ml) and incubated for 72 hours. Four hours before the end of incubation, MTT solution was added to dissolve the formed formazan crystals, and the absorbance was measured at 570 nm using a spectrophotometer. The stimulation index (SI) was calculated by dividing the absorbance of the stimulated group by the absorbance of the unstimulated group.

[0059] 4. Immunoglobulin (IgG, IgA, IgM) levels: Serum immunoglobulin concentrations were measured using an enzyme immunoassay (ELISA).

[0060] 5. Inflammation and anti-inflammatory cytokines: The concentrations of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), and interleukin-10 (IL-10) in serum were measured by enzyme immunoassay (ELISA).

[0061] 6. Evaluation of Infection Resistance: Twenty-eight days after administration, all experimental animals were intranasally inoculated with an attenuated form of canine coronavirus (cCoV) to induce artificial infection. Clinical symptoms (temperature, appetite, activity, nasal discharge, cough) were monitored and scored 10 days post-inoculation. Furthermore, viral excretions were collected via nasal swabs and quantified by real-time reverse transcription polymerase chain reaction (RT-PCR).

[0062] All experimental results are expressed as mean ± standard deviation. For statistical analysis, one-batch ANOVA and Tukey's multiple comparison test were performed. p < 0.05 was considered statistically significant.

[0063] Table 1. Effects of far-infrared oxygen on immune-related indicators

[0064] Table 1

[0065]

[0066]

[0067] *p<0.05, **p<0.01 (compared to control)

[0068] In this experiment, the effects of far-infrared oxygenated water (Example 1) and various control group samples on the immunity of companion animals were comprehensively evaluated. As a result, the far-infrared oxygenated water prepared in Example 1 showed a significantly superior immune-enhancing effect compared with all other control groups.

[0069] Compared with the control group in Example 1, NK cell activity increased by 72.3% (p<0.01), showing the most significant improvement. This means that far-infrared oxygenated water enhances the cytotoxic ability of natural killer cells, thereby improving their defense against virus-infected cells and tumor cells. An increase of 27.0% (p<0.05) was also observed in Comparative Example 3 (oxygenated water), but it did not achieve the effect of Example 1.

[0070] As a result of the measurement of lymphocyte proliferation capacity, the administration group of Implementation Example 1 showed an increase of 79.2% compared with the control group (p<0.01). This indicates that far-infrared oxygenated water significantly improved the activation and proliferation capacity of T lymphocytes, thereby enhancing the antigen-specific immune response. In Comparative Example 3 (oxygenated water), an increase of 29.2% was observed (p<0.05), but the effect was limited compared with Example 1.

[0071] Overall, compared with other samples, the far-infrared oxygenated water of Example 1 showed the best effect on all immune-related indicators. Of particular note was the significant improvement in functional activation (NK cell activity, lymphocyte proliferation capacity) and the increase in the number of immune cells. Furthermore, the reduction in inflammatory cytokines and the increase in immunomodulatory cytokines indicate that the far-infrared oxygenated water optimally modulates the immune system without producing an excessive inflammatory response.

[0072] The mechanism of immune enhancement can be inferred as follows: Far-infrared energy increases cell membrane permeability by rearranging the structure of water molecules, thereby improving the supply of nutrients and oxygen to immune cells. High concentrations of dissolved oxygen activate the energy metabolism of immune cells, optimize the balance of free radicals, and enhance immune cell function. Alkaline pH and balanced mineral composition optimize the body's acid-base balance and improve the signal transduction process of immune cells.

[0073] Of particular note is the excellent protective effect demonstrated in actual viral infection models, proving that the immunomodulatory effect of far-infrared oxygenated water directly contributes to actual pathogen defense. This comprehensive immunomodulatory effect was observed only in simple oxygenated water (Comparative Example 3) or alkaline water (Comparative Example 4), and is believed to be due to the synergistic effect of the three main properties of far-infrared oxygenated water (far-infrared energy, high oxygen concentration, and alkaline pH).

[0074] In summary, the far-infrared oxygenated water of Example 1 significantly enhances the immunity of companion animals, scientifically demonstrating increased resistance to pathogen infection. This effect is due to the optimal combination of far-infrared energy, high oxygen concentration, and alkaline pH, while only limited effects were observed in comparative examples where only one of these three factors existed alone.

[0075] Table 2

[0076]

[0077] As an analysis of the experimental results, compared with other comparative examples, the far-infrared oxygen water of Example 1 showed a significantly better reactive oxygen species inhibition effect.

[0078] Due to its DPPH radical scavenging ability, Example 1 showed a high scavenging capacity of 72.5%, which is 2.2 times that of Comparative Example 3 (32.7%) using only oxygen and 13.7 times that of Comparative Example 1 (5.3%) using ordinary pure water. These results were attributed to the synergistic effect of far-infrared energy, high oxygen concentration, and alkaline minerals.

[0079] When the total antioxidant capacity was measured by ORAC analysis, Example 1 showed excellent antioxidant capacity of 3,850 μmol TE / L. This is equivalent to 2.1 times that of Comparative Example 3 (1,840 μmol TE / L) and 9.2 times the highest value in Comparative Example 1 (420 μmol TE / L), demonstrating that Example 1 has the ability to effectively neutralize various reactive oxygen species.

[0080] In experiments measuring ROS in canine fibroblasts, Example 1 significantly reduced ROS production to 38.5% compared to the control group under H2O2-induced oxidative stress. The results showed that the ROS inhibition effect was approximately 1.6 times higher than Comparative Example 3 (62.1%) and approximately 2.5 times better than Comparative Example 1 (95.8%), indicating that Example 1 can effectively prevent oxidative damage to pet skin cells.

[0081] As a result of the analysis of antioxidant enzyme activity in canine blood, Example 1 significantly increased the activity of major antioxidant enzymes such as SOD, CAT, and GPx. In particular, the CAT activity was 95.7 U / mg protein, which is 1.5 times the highest value of Ex3 (62.3 U / mg protein) and 2.5 times that of Ex1 (38.2 U / mg protein). This result demonstrates that Example 1 has the effect of enhancing the antioxidant defense system in pets.

[0082] In summary, the far-infrared oxygenated water of Example 1 of this invention exhibits excellent reactive oxygen species inhibition through the combined effects of far-infrared energy, high concentration of oxygen, and alkaline minerals. This indicates that it can be effectively used for treating skin diseases in companion animals, anti-aging, enhancing immunity, and promoting blood health. In particular, feeding Example 1 to pets frequently suffering from atopic and skin diseases is expected to reduce oxidative stress in the body, enhance the immune system, and thus improve skin health. Furthermore, for older pets, continuous intake of Example 1 has been shown to help suppress age-related oxidative stress and maintain vitality.

Claims

1. A far-infrared oxygenated water with immune and reactive oxygen species inhibitory effects and its preparation method, characterized in that, Minerals with high far-infrared emissivity are sorted out, ground, and then subjected to contact with water under pressure to transmit far-infrared energy. After being injected with microbubble oxygen and controlled to be alkaline, the mixture is filtered, sterilized, and then packaged.

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