Anti-drop glass slide and preparation method thereof

Abstract

This invention discloses an anti-detachment glass slide and its preparation method. The preparation method includes the following steps: soaking the glass slide in a pigskin gelatin solution for a period of time; washing the soaked glass slide; and drying the washed glass slide to obtain the anti-detachment glass slide. This invention uses pigskin gelatin as a raw material to form an anti-detachment film on the glass slide, which can have an excellent anti-detachment effect on cells or tissues, while solving the problems of poor anti-detachment effect and high cost of existing anti-detachment glass slides. Furthermore, the preparation method of this invention is simple to operate and can form a uniform film on the surface of the glass slide, further improving the anti-detachment effect of the glass slide.

Description

Technical Field

[0001] This invention relates to the field of anti-detachment glass slide technology, specifically to an anti-detachment glass slide and its preparation method. Background Technology

[0002] Anti-detachment slides, also known as anti-detachment slides, ion-modified slides, and cell-capturing slides, are slides or coverslips whose surface has been modified using chemical or physical methods. They are commonly used in cell culture, pathological tissue and cell preparation, and liquid-based cytology thin-layer preparation to prevent cells or tissues from falling off during the procedure.

[0003] Anti-detachment slides are commonly used in cytology. After a specimen has been treated with a cell preservation solution, the mucus is diluted, reducing the adhesion of cells to the slide. The more diseased the cells, the greater the decrease in adhesion. Therefore, anti-detachment treatment of the slide is necessary. Insufficient slide adhesion can lead to selective cell adsorption; that is, epidermal cells with low density and high charge are easily adsorbed, while basal cells with high density and low charge, as well as diseased cells, are detached, resulting in false negative diagnoses.

[0004] Preventing slide detachment is crucial in histology, such as immunohistochemical staining, for tumor diagnosis and prognosis. Immunohistochemical staining requires tissue sections to undergo high temperature, high pressure, or enzymatic digestion and multiple washing steps. Experience has shown that slide detachment during immunohistochemical staining is influenced by many factors. Poor adhesion to the slide easily leads to detachment, wasting reagents and manpower, affecting diagnostic quality, and in some cases, irreparable damage to small specimens. Therefore, preventing slide detachment is a key step in the successful execution of immunohistochemical staining.

[0005] In existing technologies, common anti-detachment slides are mostly APES (3-aminopropyl-ethoxysilane) or poly-1-lysine. The principle is that APES adheres to the slide, and the other end of the molecule non-covalently binds to cells and tissue sections, or the poly-1-lysine (PLL) polycation molecules interact with the anions on the tissue sections, thereby generating strong adhesion. For example, the anti-detachment slide and its preparation method disclosed in Chinese patent application number CN201610988836.6, wherein the anti-detachment layer comprises APES and anhydrous ethanol. Another example is a medical adhesive slide disclosed in Chinese patent application number 201210487248.6, which includes: a wash-free slide, a poly-1-lysine layer disposed on the wash-free slide, and a labeling layer disposed on the poly-1-lysine layer. APES must be prepared and used immediately; air bubbles can easily form during slide preparation, affecting staining results. Poly-L-Lysine is expensive, and its preparation often involves applying a layer of poly-L-Lysine to the slide using cotton swabs, resulting in uneven application and affecting the effectiveness. Furthermore, proper cleaning of the slides before mounting is crucial; contaminated slides can interfere with slide mounting and lead to misdiagnosis. Poor slide pretreatment, leaving grease and other stains on the surface, can cause gaps during mounting, uneven mounting, and mottled marks after drying, making it difficult for the anti-detachment tablets to adhere and resulting in poor anti-detachment effects.

[0006] In view of this, the present invention is hereby proposed. Summary of the Invention

[0007] The primary objective of this invention is to provide an anti-detachment glass slide. This anti-detachment glass slide uses pigskin gelatin as a raw material to form an anti-detachment film on the glass slide, which can have an excellent anti-detachment effect on cells or tissues. At the same time, it solves the problems of poor anti-detachment effect and high cost of existing anti-detachment glass slides.

[0008] The second objective of this invention is to provide a method for preparing an anti-detachment glass slide. This method is simple to operate and forms a uniform thin film on the surface of the glass slide, thereby improving the anti-detachment effect of the glass slide.

[0009] In order to achieve the above-mentioned objectives of the present invention, the following technical solution is adopted:

[0010] The first aspect of this invention provides a method for preparing an anti-detachment glass slide, the method comprising the following steps:

[0011] (a) Soak the glass slide in a pigskin gelatin solution for a period of time;

[0012] (b) Clean the soaked glass slide;

[0013] (c) Dry the cleaned glass slide to obtain the anti-detachment glass slide.

[0014] The anti-shedding glass slide of the present invention uses pigskin gelatin as raw material to form a thin film on the glass slide, which can have an excellent anti-shedding effect on cells or tissues, and at the same time solves the problems of poor anti-shedding effect and high cost of existing anti-shedding glass slides.

[0015] Preferably, the concentration of pigskin gelatin in the pigskin gelatin solution is 5–20 mg / ml.

[0016] Preferably, the concentration of pigskin gelatin in the pigskin gelatin solution is 10 mg / ml.

[0017] Preferably, the soaking time is not less than 25 minutes.

[0018] Preferably, the temperature of the pigskin gelatin solution during the soaking process is room temperature.

[0019] Preferably, the cleaning agent used for cleaning is distilled water or deionized water.

[0020] Preferably, the drying temperature is 37–45°C and the drying time is 3–6 min.

[0021] The second aspect of the present invention provides an anti-detachment glass slide prepared by the above-mentioned method for preparing anti-detachment glass slides.

[0022] Compared with the prior art, the beneficial effects of the present invention include at least the following:

[0023] The preparation method of this invention uses pigskin gelatin as raw material to form an anti-detachment film on a glass slide, which can have an excellent anti-detachment effect on cells or tissues, and at the same time solves the problems of poor anti-detachment effect and high cost of existing anti-detachment glass slides.

[0024] The method for preparing the anti-detachment glass slide of the present invention is simple to operate and can form a uniform thin film on the surface of the glass slide, thereby further improving the anti-detachment effect of the glass slide. Attached Figure Description

[0025] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the accompanying drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. In all the drawings, similar elements or parts are generally identified by similar reference numerals. In the drawings, the elements or parts are not necessarily drawn to scale.

[0026] Figure 1 The results of the anti-detachment experiment of this application and commercially available anti-detachment glass slides in Experimental Example 2 of this invention are shown. Detailed Implementation

[0027] The embodiments of the technical solution of the present invention will be described in detail below with reference to the examples. The following embodiments are only used to illustrate the technical solution of the present invention more clearly, and are therefore only examples, and should not be used to limit the scope of protection of the present invention.

[0028] It should be noted that, unless otherwise stated, the technical or scientific terms used in this application should have the ordinary meaning as understood by one of ordinary skill in the art to which this invention pertains.

[0029] This invention provides a method for preparing an anti-detachment glass slide, the method comprising the following steps:

[0030] (a) Soak the glass slide in a pigskin gelatin solution for a period of time;

[0031] (b) Clean the soaked glass slide;

[0032] (c) Dry the cleaned glass slide to obtain the anti-detachment glass slide.

[0033] In the preparation method of the anti-detachment slide of the present invention, a layer of anti-detachment film is formed on the slide using pigskin gelatin as raw material. The anti-detachment film is evenly distributed and of uniform thickness, which can have an excellent anti-detachment effect on cells or tissues. At the same time, it solves the problems of poor anti-detachment effect and high cost of existing anti-detachment slides.

[0034] In some embodiments, the concentration of pigskin gelatin in the pigskin gelatin solution can be any value between 5 and 20 mg / ml, specifically 5 mg / ml, 10 mg / ml, or 20 mg / ml.

[0035] In one embodiment, the concentration of pigskin gelatin in the pigskin gelatin solution can be 10 mg / ml. By limiting the concentration of the pigskin gelatin solution, the prepared anti-detachment glass slides have a better anti-detachment effect.

[0036] In some embodiments, the soaking time is not less than 25 minutes, specifically 25 minutes, 30 minutes or 35 minutes.

[0037] In one embodiment, the temperature of the pigskin gelatin solution during the soaking process is room temperature.

[0038] In one embodiment, the cleaning agent used for cleaning is distilled water or deionized water.

[0039] In one embodiment, the drying temperature can be any value between 37 and 45°C, and the drying time can be any value between 3 and 6 minutes. Specifically, the drying temperature can be 37°C, 39°C, 40°C, or 45°C; and the drying time can be 3 minutes, 4 minutes, or 6 minutes.

[0040] Another embodiment of the present invention provides an anti-detachment glass slide prepared by the above-described method.

[0041] The technical solution of the present invention will be further described in detail below through specific embodiments.

[0042] The following raw materials are used:

[0043] Pigskin gelatin: beyotime, product number ST1339-500g.

[0044] Example 1

[0045] This embodiment is a method for preparing an anti-detachment glass slide, which includes the following steps:

[0046] (a) Remove any broken or scratched slides and insert them one by one into the slide holder. Place the slide holder and slides together into an empty staining jar. Add pigskin gelatin to water and heat slightly until the mixture is homogeneous to prepare a pigskin gelatin solution with a concentration of 5 mg / ml. Then pour the pigskin gelatin solution into the staining jar until the slides are completely submerged. Soak at room temperature for 30 minutes.

[0047] (b) Rinse the soaked glass slides clean with distilled water;

[0048] (c) The cleaned slide is placed in an oven and dried at 37°C for 6 minutes to obtain the anti-detachment slide.

[0049] Example 2

[0050] This embodiment is a method for preparing an anti-detachment glass slide, which includes the following steps:

[0051] (a) Remove any broken or scratched slides and insert them one by one into the slide holder. Place the slide holder and slide holder together into an empty staining jar. Add pigskin gelatin to water and heat slightly until the mixture is homogeneous to prepare a pigskin gelatin solution with a concentration of 10 mg / ml. Then pour the pigskin gelatin solution into the staining jar until the slides are completely submerged. Soak at room temperature for 30 minutes.

[0052] (b) Rinse the soaked glass slides clean with distilled water;

[0053] (c) The cleaned glass slide is placed in an oven and dried at 37°C for 4 minutes to obtain the anti-detachment glass slide.

[0054] Example 3

[0055] This embodiment is a method for preparing an anti-detachment glass slide, which includes the following steps:

[0056] (a) Remove any broken or scratched slides and insert them one by one into the slide holder. Place the slide holder and slide holder together into an empty staining jar. Add pigskin gelatin to water and heat slightly until the mixture is homogeneous to prepare a pigskin gelatin solution with a concentration of 15 mg / ml. Then pour the pigskin gelatin solution into the staining jar until the slides are completely submerged. Soak at room temperature for 30 minutes.

[0057] (b) Rinse the soaked glass slides clean with distilled water;

[0058] (c) The cleaned glass slide is placed in an oven and dried at 40°C for 4 minutes to obtain the anti-detachment glass slide.

[0059] Example 4

[0060] This embodiment is a method for preparing an anti-detachment glass slide, which includes the following steps:

[0061] (a) Remove any broken or scratched slides and insert them one by one into the slide holder. Place the slide holder and slide holder together into an empty staining jar. Add pigskin gelatin to water and heat slightly until the mixture is homogeneous to prepare a pigskin gelatin solution with a concentration of 20 mg / ml. Then pour the pigskin gelatin solution into the staining jar until the slide is completely submerged. Soak at room temperature for 30 minutes.

[0062] (b) Rinse the soaked glass slides clean with distilled water;

[0063] (c) The cleaned glass slide is placed in an oven and dried at 45°C for 3 minutes to obtain the anti-detachment glass slide.

[0064] Example 5

[0065] This embodiment is a method for preparing an anti-detachment glass slide, which includes the following steps:

[0066] (a) Remove any broken or scratched slides and insert them one by one into the slide holder. Place the slide holder and slide holder together into an empty staining jar. Add pigskin gelatin to water and heat slightly until the mixture is homogeneous to prepare a pigskin gelatin solution with a concentration of 10 mg / ml. Then pour the pigskin gelatin solution into the staining jar until the slide is completely submerged. Soak at room temperature for 25 minutes.

[0067] (b) Rinse the soaked glass slides clean with distilled water;

[0068] (c) The cleaned glass slide is placed in an oven and dried at 40°C for 5 minutes to obtain the anti-detachment glass slide.

[0069] Experimental Example 1

[0070] This experimental example studies the effect of different concentrations of pigskin gelatin on the performance of the prepared anti-detachment glass slides.

[0071] Porcine skin gelatin solutions with concentrations of 2 mg / ml, 5 mg / ml, 10 mg / ml, 15 mg / ml, and 20 mg / ml were prepared respectively; then, the above porcine skin gelatin solutions were used to prepare anti-detachment glass slides according to the preparation method of Example 2.

[0072] The anti-detachment performance of the anti-detachment fragments prepared above was tested using the following methods:

[0073] Take the above-mentioned anti-detachment glass slides and test the same paraffin section specimen according to the SP method, and repeat the test for detachment.

[0074] The degree of detachment is classified into three levels: mild detachment: the edge of the section is slightly curled to a detachment area of ​​<10%; moderate detachment: 10% < detachment area < 80%; severe detachment: detachment area > 80%.

[0075] Detachment refers to the slide detaching from the glass slide or the slide having separated from the glass slide and being suspended but not yet detached;

[0076] The results of the slide removal are shown in Table 1:

[0077] Table 1. Support conditions for different anti-detachment glass slides.

[0078]

[0079] As shown in Table 1:

[0080] The gelatin concentration of 10 mg / ml achieved the best anti-hair loss effect. As the concentration increased, the anti-hair loss effect did not improve further, but the cleanliness of the glass slide decreased.

[0081] Experiment Example 2

[0082] The anti-detachment slide prepared in Example 2 and a commercially available polylactic acid anti-detachment slide were used for testing.

[0083] The anti-detachment slide prepared in Example 2 and a commercially available anti-detachment slide were used to test the same paraffin section specimen using the SP method, and the test was repeated ten times. The test results are as follows: Figure 1 As shown;

[0084] Figure 1In the middle, the top 10 are commercially available poly-lysine anti-detachment glass slides, and the bottom 10 are anti-detachment glass slides prepared in Example 2 of this invention;

[0085] Depend on Figure 1 It can be known that:

[0086] The anti-detachment glass slide of the present invention effectively prevents cells or tissues from detaching from the glass slide and can better maintain the original three-dimensional morphology of cells or tissues. The anti-detachment effect is significantly better than that of commercially available products.

[0087] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features therein. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention, and they should all be covered within the scope of the claims and specification of the present invention.

Claims

1. A method for preparing an anti-detachment glass slide, characterized in that, The method for preparing the anti-detachment glass slide includes the following steps: (a) Soak the glass slide in a pigskin gelatin solution for a period of time; (b) Clean the soaked glass slide; (c) Dry the cleaned glass slide to obtain the anti-detachment glass slide.

2. The method for preparing the anti-detachment glass slide according to claim 1, characterized in that, The concentration of pigskin gelatin in the pigskin gelatin solution is 5–20 mg / ml.

3. The method for preparing the anti-detachment glass slide according to claim 1, characterized in that, The concentration of pigskin gelatin in the pigskin gelatin solution is 10 mg / ml.

4. The method for preparing the anti-detachment glass slide according to claim 1, characterized in that, The soaking time shall not be less than 25 minutes.

5. The method for preparing the anti-detachment glass slide according to claim 1, characterized in that, The temperature of the pigskin gelatin solution during the soaking process is room temperature.

6. The method for preparing the anti-detachment glass slide according to claim 1, characterized in that, The cleaning agent used is distilled water or deionized water.

7. The method for preparing the anti-detachment glass slide according to claim 1, characterized in that, The drying temperature is 37–45°C, and the drying time is 3–6 minutes.

8. The anti-detachment slide prepared by the method of any one of claims 1 to 6.

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