Angelica sinensis polysaccharide extraction method and application thereof

Abstract

This invention relates to the field of Angelica sinensis polysaccharide extraction technology, and discloses an extraction method for Angelica sinensis polysaccharides and its application, comprising the following steps: S1, preparation of raw material Angelica sinensis; S2, crushing of raw material; S3, mixing to obtain a mixture; S4, centrifuging the mixture to obtain a precipitate; S5, adding the precipitate to deionized water and stirring to dissolve, and adding acid for adjustment; S6, precipitation using 70%–90% ethanol, followed by centrifugation to obtain Angelica sinensis polysaccharides. This invention effectively breaks down the cell walls of Angelica sinensis through high-temperature heating and centrifugation, fully releasing the polysaccharide components within the cells; adjusting the pH value and using ethanol precipitation can remove impurities and improve the purity of the polysaccharides. This extraction method is simple and convenient to operate, suitable for large-scale production, and can obtain high-purity, high-quality Angelica sinensis polysaccharide products. The application of Angelica sinensis polysaccharides will further promote the development of the pharmaceutical and health food fields, providing more choices for people's health.

Description

Technical Field

[0001] This invention relates to the field of Angelica polysaccharide extraction technology, specifically to an extraction method for Angelica polysaccharides and its applications. Background Technology

[0002] Angelica sinensis is a perennial herb belonging to the genus Angelica in the family Apiaceae. Its roots are cylindrical, branched, and yellowish-brown; the stems are erect, greenish-white or purplish, with deep longitudinal grooves; the leaves are pinnately divided, purple or green, and ovate; the flowers are white, with pedicels densely covered in fine hairs, petals oblong-ovate, and a conical styla base; flowering occurs from June to July. The fruit is elliptical to ovate, with pale purple wing margins; fruiting occurs from July to September.

[0003] Angelica sinensis is one of the important traditional Chinese medicines, containing a variety of active ingredients and possessing various pharmacological effects, such as nourishing blood, promoting blood circulation, and regulating menstruation. Angelica sinensis polysaccharide is a polysaccharide compound extracted from it. Angelica sinensis is a common Chinese medicinal material that is widely used in traditional Chinese medicine. Angelica sinensis polysaccharide has a variety of biological activities and pharmacological effects. In recent years, studies have found that the polysaccharide components in Angelica sinensis have a variety of biological activities, such as antioxidant, anti-inflammatory, and anti-tumor effects.

[0004] Existing methods for extracting Angelica polysaccharides suffer from low extraction efficiency and low purity, which limits their application in medicine, health food, and cosmetics. Therefore, this paper proposes an extraction method for Angelica polysaccharides and its application. Summary of the Invention

[0005] The purpose of this invention is to provide a method for extracting Angelica polysaccharides and its application, in order to solve the problems of low extraction efficiency and low purity in existing Angelica polysaccharide extraction methods mentioned in the background art, which limit its application in the fields of medicine, health food and cosmetics.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a method for extracting Angelica sinensis polysaccharides, comprising the following steps;

[0007] S1. Preparation of raw material Angelica sinensis;

[0008] S2, Raw material crushing;

[0009] S3. Mix to obtain a mixture;

[0010] S4. Centrifuge the mixture to obtain the precipitate;

[0011] S5. Add the precipitate to deionized water and stir to dissolve, then add acid to adjust the consistency.

[0012] S6. Use 70% to 90% ethanol for precipitation, then centrifuge to obtain Angelica polysaccharide.

[0013] Preferably, in step S1, the weight of the prepared Angelica sinensis raw material is 500-1000g.

[0014] Preferably, in step S2, the raw material is crushed into Angelica sinensis powder, which is then passed through an 80-mesh sieve to obtain Angelica sinensis powder.

[0015] Preferably, in step S3, the Angelica sinensis powder is mixed with distilled water at a ratio of 1:10.

[0016] Preferably, the mixture is heated to 70–90°C and maintained for 1–3 hours.

[0017] Preferably, in step S4, the mixture is separated by centrifugation using a centrifuge at a speed of 2000–5000 rpm for 10–30 minutes.

[0018] Preferably, in step S5, the acid used is an organic or inorganic acid, and the pH value is between 5.0 and 6.0.

[0019] Preferably, the deionized water mentioned above is pure water or deionized water.

[0020] Preferably, in step S6, the centrifugation speed is 2200–4800 rpm and the centrifugation time is 15–33 minutes.

[0021] Preferably, the aforementioned Angelica polysaccharides can be used in health products and pharmaceuticals. The extracted Angelica polysaccharides are mixed with cosmetic raw materials to prepare emulsions, creams, and other cosmetics for moisturizing, anti-aging, and other skincare functions. Angelica polysaccharides can enhance the skin's moisturizing ability, improve skin elasticity and firmness, and reduce the appearance of fine lines and wrinkles, thereby achieving skincare effects. As a natural product with various biological activities, Angelica polysaccharides have broad application prospects in the pharmaceutical and health food fields. By mixing with suitable excipients to prepare different dosage forms, it can meet people's needs for treating diseases, enhancing immunity, and anti-aging. The application of Angelica polysaccharides will further promote the development of the pharmaceutical and health food fields, providing more choices for people's health.

[0022] Compared with the prior art, the present invention, employing the above technical solution, has the following technical effects:

[0023] I. This invention effectively breaks down the cell walls of Angelica sinensis through high-temperature heating and centrifugation, fully releasing the polysaccharide components within the cells. Adjusting the pH value and using ethanol precipitation removes impurities and improves the purity of the polysaccharides. This extraction method is simple and convenient to operate, suitable for large-scale production, and can yield high-purity, high-quality Angelica sinensis polysaccharide products.

[0024] II. The Angelica polysaccharides extracted in this invention are mixed with cosmetic raw materials to prepare emulsions, creams, and other cosmetics for moisturizing, anti-aging, and other skincare functions. Angelica polysaccharides can enhance the skin's moisturizing ability, improve skin elasticity and firmness, and reduce the appearance of fine lines and wrinkles, thereby achieving skincare effects. As a natural product with multiple biological activities, Angelica polysaccharides have broad application prospects in the fields of pharmaceuticals and health foods. By mixing with suitable excipients to prepare different dosage forms, it can meet people's needs for treating diseases, enhancing immunity, and anti-aging. The application of Angelica polysaccharides will further promote the development of the pharmaceutical and health food fields and provide people with more choices for health. Attached Figure Description

[0025] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0026] Figure 1 This is a schematic diagram of the process of the present invention. Detailed Implementation

[0027] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0028] It should be noted that the structures, proportions, sizes, etc., shown in the accompanying drawings of this specification are only for the purpose of assisting those skilled in the art in understanding and reading the content disclosed in the specification, and are not intended to limit the conditions under which this application can be implemented. Therefore, they have no substantial technical significance. Any modifications to the structure, changes in the proportions, or adjustments to the size should still fall within the scope of the technical content disclosed in this application, provided that they do not affect the effects and purposes that this application can produce.

[0029] Existing methods for extracting angelica polysaccharides suffer from low extraction efficiency and low purity, which limits their application in the fields of medicine, health food, and cosmetics.

[0030] Example 1

[0031] Please see Figure 1 The present invention provides a technical solution: a method for extracting Angelica polysaccharides, comprising the following steps;

[0032] S1. Preparation of raw material Angelica sinensis; The weight of raw material Angelica sinensis is 500g. Clean the Angelica sinensis to remove mud and other impurities. In the preparation process, the Angelica sinensis must be cleaned first. In order to remove mud, pesticide residues and other impurities and ensure the smooth progress of the subsequent extraction process, you can use running water or appropriate cleaning agent to clean the Angelica sinensis, and then wipe it dry with clean gauze or paper towel.

[0033] In addition to cleaning, Angelica sinensis can be pre-treated by slicing, crushing or pulverizing, depending on actual needs, in order to increase the extraction area of ​​polysaccharides and improve extraction efficiency. The pre-treatment process should be as gentle as possible to avoid excessive heating or oxidation in order to maintain the biological activity of polysaccharides.

[0034] S2. Raw material crushing: The raw material is Angelica sinensis powder, which is obtained by passing through an 80-mesh sieve. Crushing can break the cell walls of Angelica sinensis, which is conducive to the release of polysaccharide components. During the crushing process, excessive heating or oxidation should be avoided to maintain the biological activity of polysaccharides. In order to improve the crushing effect and efficiency, some auxiliary agents, such as cellulase or pectinase, can be added in appropriate amounts to promote the decomposition of cell walls.

[0035] The crushed angelica powder should be stored in time to avoid problems such as moisture, mold or oxidation that affect the quality of polysaccharide extraction. During storage, choose a dry, cool and well-ventilated place, and pay attention to moisture-proof, insect-proof and sun-proof measures.

[0036] S3. Mixing to obtain a mixture: Mix Angelica sinensis powder and distilled water in a 1:10 ratio. Heat the mixture to 70°C and maintain for 1 hour. Heating allows the polysaccharide components within the Angelica sinensis cells to fully dissolve. During mixing, ensure sufficient contact between the Angelica sinensis powder and the solvent to promote polysaccharide dissolution. Stirring or ultrasonic oscillation can be used to ensure a homogeneous mixture free of sediment. The mixture then requires further heating to promote polysaccharide dissolution and diffusion. Heating to 70°C and maintaining for 1 hour increases the solvent temperature, accelerating molecular motion and promoting polysaccharide dissolution from the cell walls. It is important to note that the heating temperature should not be too high to avoid damaging the biological activity of the polysaccharides or causing excessive solvent evaporation. Maintaining appropriate temperature and time ensures sufficient polysaccharide dissolution and improves extraction efficiency.

[0037] S4. Centrifuge the mixture to obtain a precipitate. Centrifuge the mixture at 2000 rpm for 10 minutes to separate the mixture into an upper liquid layer and a lower precipitate layer. Centrifugation removes residues and other insoluble impurities. The upper liquid layer mainly contains solvents and other soluble components, while the lower precipitate is the extracted Angelica polysaccharide. Centrifugation removes residues and other insoluble impurities, improving the purity of the extract. The precipitate after centrifugation requires further processing and purification to obtain higher quality Angelica polysaccharides.

[0038] S5. Add the precipitate to deionized water and stir to dissolve. Adjust the pH to 5.0 by adding acid (organic or inorganic). Use pure water or deionized water. Transfer the supernatant after centrifugation to another container and adjust the pH to 5.0 by adding acid (common organic acids such as acetic acid) or inorganic acids such as hydrochloric acid). Adjusting the pH promotes the precipitation of polysaccharides from the solution. Adding the precipitate to deionized water removes ions from the solution, which is beneficial for subsequent purification. Adjusting the pH promotes the precipitation of polysaccharides. When the pH is close to the isoelectric point of the polysaccharides, the repulsive force between polysaccharide molecules weakens, making it easier for precipitation to form. By adjusting the pH, the polysaccharides can be separated from the solution as precipitates, improving the purity of the extract. Deionized water also plays a crucial role in dissolving the precipitate. Due to its extremely low ion content, it reduces the ion concentration in the solution, minimizing interference from ions on the polysaccharides and facilitating subsequent purification.

[0039] S6. Precipitation was performed using 70% ethanol, followed by centrifugation to obtain Angelica polysaccharides. The centrifugation speed was 2200 rpm for 15 minutes. Ethanol precipitation further removes impurities and improves the purity of the polysaccharides. After precipitation, the mixture was centrifuged at 2200 rpm for 15 minutes to generate sufficient centrifugal force between the precipitate and the solvent, thus achieving better separation. After centrifugation, the precipitate is relatively pure Angelica polysaccharides. The upper liquid portion may contain other soluble impurities, which can be further processed or discarded. Ethanol precipitation further removes impurities and improves the purity of the polysaccharides. This method is crucial for obtaining high-quality Angelica polysaccharide extracts.

[0040] High-temperature heating and centrifugation can effectively break down the cell walls of Angelica sinensis, fully releasing the polysaccharide components within the cells. Adjusting the pH value and using ethanol precipitation can remove impurities and improve the purity of the polysaccharides. This extraction method is simple and convenient to operate, suitable for large-scale production, and can obtain high-purity, high-quality Angelica sinensis polysaccharide products.

[0041] Example 2

[0042] The difference between this embodiment and Embodiment 1 is that:

[0043] A method for extracting Angelica sinensis polysaccharides includes the following steps;

[0044] S1. Preparation of raw material Angelica sinensis; The weight of raw material Angelica sinensis is 600g;

[0045] S2. Raw material crushing; The raw material is angelica powder, which is obtained by passing it through an 80-mesh sieve.

[0046] S3. Mix to obtain a mixture; mix angelica powder and distilled water in a ratio of 1:10, heat the mixture to 78°C and maintain for 1.3 hours;

[0047] S4. Centrifuge the mixture to obtain the precipitate; the mixture is separated by centrifugation using a centrifuge at a speed of 2300 rpm for 13 minutes.

[0048] S5. Add the precipitate to deionized water and stir to dissolve. Add acid to adjust the pH to 5.3. The acid used can be organic or inorganic. The deionized water can be pure water or deionized water.

[0049] S6. Precipitate with 75% ethanol, then centrifuge to obtain Angelica polysaccharide. The centrifugation speed is 2800 rpm and the centrifugation time is 18 minutes.

[0050] Example 3

[0051] The difference between this embodiment and Embodiments 1 and 2 is that:

[0052] A method for extracting Angelica sinensis polysaccharides includes the following steps;

[0053] S1. Preparation of raw material Angelica sinensis; The weight of raw material Angelica sinensis is 800g;

[0054] S2. Raw material crushing; The raw material is angelica powder, which is obtained by passing it through an 80-mesh sieve.

[0055] S3. Mix to obtain a mixture; mix angelica powder and distilled water in a ratio of 1:10, heat the mixture to 80°C and maintain for 2 hours;

[0056] S4. Centrifuge the mixture to obtain the precipitate; the mixture is separated by centrifugation using a centrifuge at a speed of 3500 rpm for 20 minutes.

[0057] S5. Add the precipitate to deionized water and stir to dissolve. Add acid to adjust the pH to 5.5. The acid used can be organic or inorganic. The deionized water can be pure water or deionized water.

[0058] S6. Use 70%–90% ethanol for precipitation, then centrifuge to obtain Angelica polysaccharide. The centrifugation speed is 3000 rpm and the centrifugation time is 28 minutes.

[0059] Example 4

[0060] The difference between this embodiment and Embodiments 1, 2, and 3 is that:

[0061] A method for extracting Angelica sinensis polysaccharides includes the following steps;

[0062] S1. Preparation of raw material Angelica sinensis; The weight of raw material Angelica sinensis is 1000g.

[0063] S2. Raw material crushing; the raw material is crushed as Angelica sinensis powder, which is obtained by passing through an 80-mesh sieve.

[0064] S3. Mix to obtain a mixture; mix angelica powder and distilled water in a ratio of 1:10, heat the mixture to 90°C and keep it for 3 hours.

[0065] S4. Centrifuge the mixture to obtain the precipitate; the mixture is separated by centrifugation using a centrifuge at a speed of 5000 rpm for 30 minutes.

[0066] S5. Add the precipitate to deionized water and stir to dissolve. Add acid to adjust the pH to 6.0. The acid used can be organic or inorganic, and the deionized water can be pure water or deionized water.

[0067] S6. Precipitate with 90% ethanol, then centrifuge to obtain Angelica polysaccharide. The centrifugation speed is 4800 rpm and the centrifugation time is 33 minutes.

[0068] This invention also provides an application of a method for extracting Angelica polysaccharides, which can be used in health products and pharmaceuticals.

[0069] Angelica polysaccharides possess antioxidant, anti-inflammatory, and anti-tumor biological activities, and can be used to prepare antioxidants, anti-inflammatory drugs, anti-tumor drugs, and other medications. In the health food industry, Angelica polysaccharides have effects such as enhancing immunity and anti-aging, and can be used to prepare health foods.

[0070] The extracted Angelica polysaccharides are mixed with excipients and prepared into tablets, capsules and other dosage forms for the treatment of oxidative stress-related diseases, inflammatory diseases, tumors and other diseases. The antioxidant and anti-inflammatory effects of Angelica polysaccharides can help reduce cellular oxidative damage and inflammatory response, thereby promoting the treatment and recovery of diseases.

[0071] The extracted angelica polysaccharides are mixed with health food raw materials and prepared into dosage forms such as capsules and granules for health functions such as enhancing immunity and anti-aging. Angelica polysaccharides can regulate the function of the immune system, improve the body's disease resistance, and have anti-aging effects, which help to delay the aging process of cells.

[0072] Extracted Angelica polysaccharides are mixed with cosmetic raw materials to prepare emulsions, creams, and other cosmetics for moisturizing, anti-aging, and other skincare functions. Angelica polysaccharides can enhance the skin's moisturizing ability, improve skin elasticity and firmness, and reduce the appearance of fine lines and wrinkles, thus achieving skincare effects. As a natural product with various biological activities, Angelica polysaccharides have broad application prospects in the pharmaceutical and health food fields. By mixing with suitable excipients to prepare different dosage forms, it can meet people's needs for treating diseases, enhancing immunity, and anti-aging. The application of Angelica polysaccharides will further promote the development of the pharmaceutical and health food fields, providing people with more choices for health.

[0073] The embodiments of the present invention have been described in detail above with reference to the accompanying drawings. It should be noted that implementations not illustrated or described in the drawings or the main text of the specification are forms known to those skilled in the art and are not described in detail. Furthermore, the definitions of the components described above are not limited to the various specific structures, shapes, or methods mentioned in the embodiments, and those skilled in the art can easily modify or substitute them.

[0074] The specific embodiments described above further illustrate the purpose, technical solution, and beneficial effects of the present invention. It should be understood that the above descriptions are merely specific embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A method for extracting Angelica sinensis polysaccharides, characterized in that: Includes the following steps; S1. Preparation of raw material Angelica sinensis; S2, Raw material crushing; S3. Mix to obtain a mixture; S4. Centrifuge the mixture to obtain the precipitate; S5. Add the precipitate to deionized water and stir to dissolve, then add acid to adjust the consistency. S6. Use 70% to 90% ethanol for precipitation, then centrifuge to obtain Angelica polysaccharide.

2. The method for extracting Angelica polysaccharides according to claim 1, characterized in that: In S1, the weight of the prepared Angelica sinensis raw material is 500-1000g.

3. The method for extracting Angelica polysaccharides according to claim 1, characterized in that: In S2, the raw material is crushed into Angelica sinensis powder, which is then passed through an 80-mesh sieve to obtain Angelica sinensis powder.

4. The method for extracting Angelica polysaccharides according to claim 1, characterized in that: In S3, the Angelica powder is mixed with distilled water at a ratio of 1:

10.

5. The method for extracting Angelica polysaccharides according to claim 4, characterized in that: The mixture is heated to 70–90°C and maintained for 1–3 hours.

6. The method for extracting Angelica polysaccharides according to claim 1, characterized in that: In S4, the mixture is separated by centrifugation using a centrifuge at a speed of 2000–5000 rpm for 10–30 minutes.

7. The method for extracting Angelica polysaccharides according to claim 1, characterized in that: In S5, the acid used is an organic or inorganic acid, and the pH value is between 5.0 and 6.

0.

8. The method for extracting Angelica sinensis polysaccharides according to claim 7, characterized in that: The deionized water is either pure water or deionized water.

9. The method for extracting Angelica polysaccharides according to claim 1, characterized in that: In S6, the centrifugation speed is 2200-4800 rpm, and the centrifugation time is 15-33 minutes.

10. The application of the method for extracting Angelica sinensis polysaccharides according to any one of claims 1 to 9, characterized in that: The Angelica polysaccharide can be used in health products and pharmaceuticals.

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