A method for preparing a postbiotic by fermenting wheat B starch pulp and bran with genetically engineered saccharomyces cerevisiae

Abstract

This invention belongs to the field of microbial fermentation and functional food technology, and relates to a method for preparing post-biotics using genetically engineered Saccharomyces cerevisiae fermentation of wheat B starch paste and wheat bran. The genetically engineered Saccharomyces cerevisiae integrates the gmd, wcaG, Lac12, and wbgL genes into its chromosome, thereby acquiring the ability to synthesize 2'-fucosylated lactose. The method includes: gelatinizing and enzymatically hydrolyzing the wheat B starch paste; mixing the hydrolyzed wheat B starch paste with wheat bran and sterilizing; inoculating with the genetically engineered Saccharomyces cerevisiae for fermentation; and after fermentation, performing solid-liquid separation, cell lysis, and freeze-drying of the fermentation system to obtain the post-biotic product. The post-biotic contains one or more active ingredients selected from 2'-fucosylated lactose, mannan, extracellular polysaccharides, and short-chain fatty acids. Animal experiments have shown that this post-biotic has the effects of alleviating diarrhea-related symptoms, improving intestinal barrier function, and regulating inflammatory factor levels, achieving high-value utilization of wheat processing by-products.

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