Method for culturing and using corrosion inhibitor of circulating water coupled with intermittent aeration and magnetic field strengthening

Abstract

This invention discloses a method for cultivating and using an intermittent aeration coupled with a magnetic field to enhance the preservative activity of circulating water bacteria, relating to the field of water treatment technology. The cultivation method involves mixing a compound bacterial agent with magnetite or maghemite in a culture medium, adding nutrients, and applying an alternating magnetic field during cultivation while using intermittent aeration to obtain the preservative agent. The application method involves adding the preservative agent to a circulating water system, periodically monitoring the comprehensive indicators of the agent in the system, and supplementing with nutrients, magnetite or maghemite, or the preservative agent when the indicators fall below a preset threshold. This invention significantly enhances the metabolic activity and anti-corrosion performance of the bacterial agent by applying an alternating magnetic field and combining it with magnetic metal oxides during cultivation, achieving a corrosion inhibition rate of over 95% for Q235 carbon steel. Simultaneously, through precise control of multiple comprehensive indicators, it ensures the long-term stable operation of the circulating water system, featuring green environmental protection and simple operation.

Description

Technical Field

[0001] This invention belongs to the field of water treatment technology, specifically relating to a method for cultivating and using a circulating water preservative agent enhanced by intermittent aeration coupled with a magnetic field. Background Technology

[0002] The circulating cooling water system of a thermal power plant is a critical link in the plant's thermal cycle, and its operational stability directly affects the economy and safety of the generating units. This system operates under dynamic conditions of high salinity, high alkalinity, and evaporation concentration for extended periods, posing a severe threat of corrosion to metal pipes and heat exchange equipment, such as carbon steel. Corrosion failure is one of the core common challenges restricting the long-term safe operation of thermal power units.

[0003] Currently, the mainstream technology for corrosion prevention in circulating water systems both domestically and internationally is primarily chemical, which involves adding scale and corrosion inhibitors to suppress metal corrosion. Traditional phosphorus-containing corrosion inhibitors pose an environmental risk of eutrophication, while phosphorus-free or low-phosphorus polymer-based corrosion inhibitors, which have been promoted in recent years, suffer from drawbacks such as high cost and poor scale and corrosion inhibition effects. Against this backdrop, microbial corrosion prevention technology has gained attention due to its environmentally friendly and sustainable characteristics. Its basic principle is to utilize functional microorganisms to form a biofilm barrier on the metal surface, secrete scale and corrosion inhibitors, and alter the surface potential of the metal to delay corrosion. However, existing microbial preservation technologies still face many bottlenecks: First, the preservation efficiency of naturally selected or conventionally cultured strains is generally low, making it difficult to meet the stringent requirements of industrial circulating water systems; second, preservative bacteria are mostly added directly, and there is a general lack of effective cultivation methods, resulting in poor activity of the bacteria and poor adaptability to water quality fluctuations; third, the addition of nutrients relies entirely on experience, lacking quantitative dosage guidelines, with the dosage potentially being too low when the bacterial abundance is high and too high when the bacterial abundance is low; fourth, after the bacteria are added to the circulating water system, there is a lack of effective methods for monitoring and precisely controlling the bacterial content, and on-site applications are often based on experience, making it difficult to guarantee the long-term stability of the protective effect. Summary of the Invention

[0004] In order to overcome the shortcomings of the prior art, the present invention aims to provide a method for cultivating and using intermittent aeration coupled with magnetic field to enhance the antiseptic agent in circulating water, so as to solve the technical problems in the existing circulating water microbial antiseptic technology, such as low antiseptic efficiency of the agent, lack of effective cultivation methods, lack of quantitative basis for nutrient addition, and lack of precise control methods after the agent is added, resulting in unstable protective effect.

[0005] To achieve the above objectives, the present invention employs the following technical solution: In a first aspect, the present invention provides a method for cultivating a preservative agent in circulating water enhanced by intermittent aeration coupled with a magnetic field, comprising the following steps: S1, mix the compound bacterial agent with magnetite or maghemite in the culture medium, and add nutrients to the culture medium; S2, During the cultivation process, an alternating magnetic field is applied to the culture medium, and air is introduced into the culture medium in an intermittent aeration manner, wherein the intermittent aeration is a periodic alternation between aeration and aeration. S3, after cultivation, the sludge precipitate in the culture medium is collected to obtain the intermittent aeration coupled magnetic field enhanced circulating water antiseptic agent.

[0006] A further improvement of the present invention is that the compound microbial agent comprises the following components by mass: 2-10 parts of Burkholderia, 5-8 parts of Pyriformis, 5-8 parts of Plankton, 1-5 parts of Lactobacillus plantarum, 1-5 parts of Saccharomyces cerevisiae, 1-3 parts of Bacillus cereus and 1-3 parts of Bacillus subtilis.

[0007] A further improvement of the present invention is that the nutrient agent comprises, by mass parts, the following components: 1-3 parts anhydrous sodium acetate, 1-2 parts glucose, 1-2 parts ammonium citrate, 1-2 parts ammonium sulfate, 1-2 parts sodium chloride, 1 part potassium dihydrogen phosphate, 0.5-1 part manganese sulfate·7H2O, and 0.5 parts beef extract.

[0008] A further improvement of the present invention is that the amount of magnetite or maghemite added to the culture solution is 5~20 mg / L.

[0009] A further improvement of the present invention is that the temperature of the culture medium is controlled at 25~40℃ during the culture process.

[0010] A further improvement of the present invention is that the magnetic field strength of the alternating magnetic field is 5~60 mT.

[0011] A further improvement of the present invention is that the aeration flow rate of the intermittent aeration is 0.1~0.3 L / min, and the aeration frequency is 1 hour of aeration followed by 3 hours of aeration cessation.

[0012] A further improvement of the present invention is that the culture time is 7 to 14 days.

[0013] Secondly, the present invention also provides a method for using an intermittent aeration coupled with a magnetic field to enhance the use of a circulating water preservative agent, comprising the following steps: The preservative agent was obtained by culturing using the above-described method; The preservative agent is added to the circulating water system; The comprehensive index of the preservative agent in the circulating water system is detected. When the comprehensive index is lower than the preset threshold, nutrients, magnetite or maghematite, or preservative agent are added accordingly.

[0014] A further improvement of the present invention is that, based on the total volume of the circulating water system, the dosage of the preservative agent is 0.1% to 1%; the preset threshold and replenishment method are as follows: When OD600 < 0.02, add preservative agent; When OD600 ≥ 0.02 and oxygen consumption rate < 15 mg / (g MLVSS·h), supplement with nutrients; When OD600 ≥ 0.02, oxygen consumption rate ≥ 15 mg / (g MLVSS·h), and qPCR detection function of microbial copy number < 10 6 When the volume is in copies / μL, supplement with magnetite or maghemite.

[0015] Compared with the prior art, the present invention has the following beneficial effects: This invention provides a method for cultivating a circulating water preservative agent using intermittent aeration coupled with a magnetic field. The method involves mixing a specific compound bacterial agent with magnetite or maghematite in a culture medium and adding a special nutrient solution. Simultaneously, an alternating magnetic field is applied during cultivation, and intermittent aeration is employed. Magnetic metal oxides are used to power the functional microorganisms to synthesize functional enzymes. The intermittent aeration process drives a Fe(III) / Fe(II) cycle to enhance the microorganisms' utilization of magnetic metal oxides, while the alternating magnetic field enhances the activity of functional enzymes. Through the scientific coupling of these three enhancement methods, the metabolic activity and proliferation efficiency of the compound bacterial agent are significantly improved. Experimental data show that the compound bacterial agent cultured under alternating magnetic field and intermittent aeration achieved an OD600 value exceeding 0.056 within 10 days, fluctuating around 0.09 between 14 and 21 days. In contrast, the control group without magnetic field and intermittent aeration treatment only reached an OD600 value of 0.04 within 14 days, fluctuating around 0.05 between 14 and 21 days. The cultivation period was significantly shortened, and the bacterial agent content was greatly increased.

[0016] This invention also provides a method for using an intermittent aeration coupled with a magnetic field to enhance the application of a circulating water preservative agent. The preservative agent, obtained using the above-mentioned cultivation method, is added to the circulating water system, allowing the agent to effectively colonize within the system and stably exert its anti-corrosion function. Experimental data shows that the Q235 carbon steel hanging plates treated by this method retain their metallic luster, with only slight corrosion marks visible in some areas. The corrosion rate decreases from 0.8982 mm / a in the untreated case to 0.0448 mm / a, achieving a corrosion inhibition rate of 95.09%. The anti-corrosion effect is significantly better than existing technologies.

[0017] This invention comprehensively reflects the quantity, activity, and abundance of functional microorganisms in a circulating water system by analyzing three indicators: OD600, oxygen consumption rate, and qPCR. It then employs targeted replenishment strategies based on the decay of different indicators, rather than blindly supplementing a single material, thereby achieving precise judgment and scientific control of the system's operating status. This control strategy is simple to operate, providing clear guidance for scientific control and precise operation on-site. It effectively ensures the long-term stable operation of the circulating water system's corrosion prevention function, avoids fluctuations in protective effect, and possesses good practicality and prospects for industrial application.

[0018] Compared with traditional continuous aeration, the intermittent aeration mode adopted in this invention significantly reduces energy consumption while ensuring sufficient oxygen supply. Furthermore, by using intermittent aeration to drive the Fe(III) / Fe(II) cycle of magnetite or maghemite, it induces extracellular electron transfer in functional microorganisms, which is beneficial to maintaining the high activity and long-term stability of the bacterial agent, and has good economic and environmental benefits. Attached Figure Description

[0019] The accompanying drawings described herein are for illustrative purposes only and are not intended to limit the scope of the invention in any way. Furthermore, the shapes and proportions of the components in the drawings are merely illustrative to aid in understanding the invention and do not specifically limit the shapes and proportions of the components of the invention.

[0020] Figure 1 This is a flowchart of a method for cultivating and using an intermittent aeration coupled with a magnetic field to enhance the antiseptic agent in circulating water according to the present invention; Figure 2 This is an effect diagram of a method for cultivating preservative bacteria in circulating water enhanced by intermittent aeration coupled with a magnetic field in Embodiment 1 of the present invention; Figure 3 The corrosion rate of Q235 carbon steel under the influence of the anti-corrosion agent of this invention. Detailed Implementation

[0021] To enable those skilled in the art to understand the features and effects of the present invention, the terms and expressions used in the specification and claims are explained and defined in general below. Unless otherwise specified, all technical and scientific terms used herein have the ordinary meaning understood by those skilled in the art regarding the present invention, and in case of conflict, the definitions in this specification shall prevail.

[0022] The theories or mechanisms described and disclosed herein, whether right or wrong, should not in any way limit the scope of the invention, that is, the contents of the invention can be implemented without being limited by any particular theory or mechanism.

[0023] In this document, all features defined by numerical ranges or percentage ranges, such as numerical values, quantities, contents, and concentrations, are for the sake of brevity and convenience only. Accordingly, descriptions of numerical ranges or percentage ranges should be considered as covering and specifically disclosing all possible sub-ranges and individual numerical values ​​(including integers and fractions) within those ranges.

[0024] In this article, unless otherwise specified, “contains,” “includes,” “containing,” “has,” or similar terms cover the meanings of “composed of” and “mainly composed of,” for example, “A contains a” covers the meanings of “A contains a and others” and “A contains only a.”

[0025] For the sake of brevity, not all possible combinations of the technical features in each implementation scheme or embodiment are described herein. Therefore, as long as there is no contradiction in the combination of these technical features, the technical features in each implementation scheme or embodiment can be combined arbitrarily, and all possible combinations should be considered within the scope of this specification.

[0026] This invention provides a method for cultivating preservative-preserving bacteria in circulating water with intermittent aeration coupled with a magnetic field enhancement, comprising the following steps: S1, the compound bacterial agent is mixed with magnetite or maghematite in a culture medium, and nutrients are added to the culture medium. The compound bacterial agent, by weight, comprises the following components: 2-10 parts of Burkholderia, 5-8 parts of Pyriformis, 5-8 parts of Plankton, 1-5 parts of Lactobacillus plantarum, 1-5 parts of Saccharomyces cerevisiae, 1-3 parts of Bacillus cereus, and 1-3 parts of Bacillus subtilis. The nutrients, by weight, comprise the following components: 1-3 parts of anhydrous sodium acetate, 1-2 parts of glucose, 1-2 parts of ammonium citrate, 1-2 parts of ammonium sulfate, 1-2 parts of sodium chloride, 1 part of potassium dihydrogen phosphate, 0.5-1 part of manganese sulfate·7H2O, and 0.5 parts of beef extract. The dosage of magnetite or maghematite in the culture medium is 5-20 mg / L.

[0027] S2, During the cultivation process, an alternating magnetic field is applied to the culture medium, and air is introduced into the culture medium through intermittent aeration. The intermittent aeration involves periodically alternating between aeration and cessation. Specifically, the temperature of the culture medium is controlled at 25~40℃ during the cultivation process; the magnetic field strength of the alternating magnetic field is 5~60 mT, preferably 40~50 mT; the aeration flow rate of the intermittent aeration is 0.1~0.3 L / min, and the aeration frequency is 1 hour of aeration followed by 3 hours of cessation.

[0028] S3, after being cultured for 7-14 days, the sludge sediment in the culture medium is collected to obtain the intermittent aeration coupled magnetic field enhanced circulating water antiseptic agent.

[0029] This invention also provides a method for using an intermittent aeration coupled with a magnetic field-enhanced circulating water preservative agent, comprising the following steps: culturing the preservative agent using the above-described culture method; adding the preservative agent to a circulating water system; detecting the comprehensive index of the preservative agent in the circulating water system; and when the comprehensive index is lower than a preset threshold, supplementing with nutrients, magnetite or maghemite, or the preservative agent accordingly. The dosage of the preservative agent is 0.1% to 1% based on the total volume of the circulating water system.

[0030] The comprehensive index detection of the high-efficiency microbial agent is carried out using a combination of methods: the content of microorganisms in the circulating water system is determined by the OD600 method; the oxygen consumption per unit time of a quantitative sample of circulating water is measured to determine the activity of microorganisms; and the copy number of functional microorganisms is quantitatively detected by qPCR. The preset thresholds and replenishment methods are as follows: when OD600 < 0.02, it indicates that the content of microbial agent in the circulating water system is insufficient, and preservative agent is added; when OD600 ≥ 0.02 and oxygen consumption rate < 15 mg / (g MLVSS·h), it indicates that the metabolic activity of the microbial agent has decreased due to insufficient nutrition, and nutrient supplementation is added; when OD600 ≥ 0.02, oxygen consumption rate ≥ 15 mg / (g MLVSS·h), and the copy number of functional microorganisms detected by qPCR is < 10... 6 When the number of copies / μL is low, it indicates that the magnetic metal oxides required for the synthesis of functional enzymes by functional microorganisms are insufficient. In this case, magnetite or maghemite should be added.

[0031] This invention enhances microbial metabolism through the synergistic effect of magnetic field and intermittent aeration, significantly improving the anti-corrosion performance of the bacterial agent. It is easy to operate and suitable for long-term protection of circulating water systems.

[0032] The present invention will be further illustrated below with reference to specific embodiments. It should be understood that these embodiments are for illustrative purposes only and are not intended to limit the scope of the invention. Furthermore, it should be understood that after reading the teachings of this invention, those skilled in the art can make various alterations or modifications to the invention, and these equivalent forms also fall within the scope defined by the appended claims.

[0033] The following examples use instruments and equipment conventional in the art. Experimental methods in the following examples, unless otherwise specified, are generally performed under conventional conditions or as recommended by the manufacturer. All raw materials used in the following examples are conventional commercially available products with specifications conventional in the art. In this specification and the following examples, unless otherwise specified, "%" represents weight percentage, "parts" represents parts by weight, and "ratio" represents weight proportion.

[0034] Example 1 This embodiment provides a method for cultivating preservative-preserving bacteria in circulating water enhanced by intermittent aeration coupled with a magnetic field, comprising the following steps: (1) Microbial culture The compound microbial agent, comprising, by weight parts, 2 parts Burkholderia, 5 parts Pyridobacterium tumefaciens, 5 parts Planktonic mold, 1 part Lactobacillus plantarum, 1 part Saccharomyces cerevisiae, 1 part Bacillus cereus, and 1 part Bacillus subtilis. Magnetite is added to the reactor at a dosage of 20 mg / L. Nutrients are added daily, comprising, by weight parts, 1 part anhydrous sodium acetate and glucose (C6H2O). 12 1 part of O6, 1 part of ammonium citrate, 1 part of ammonium sulfate ((NH4)2SO4), 1 part of sodium chloride, 1 part of potassium dihydrogen phosphate (KH2PO4), 0.5 part of manganese sulfate·7H2O, and 0.5 part of beef extract.

[0035] The reactor temperature was maintained at 35℃. During the cultivation process, an alternating magnetic field of 40mT was applied to the reactor, and the growth of microorganisms was stimulated by intermittent aeration. The aeration flow rate was 0.2L / min, and the aeration frequency was 1 hour of aeration followed by 3 hours of aeration. The culture was carried out continuously for 10 days, and the sludge sediment in the culture medium was collected to obtain the preservative agent.

[0036] (2) Test results During cultivation, the change in bacterial concentration over time was measured using OD600, and the results are as follows: Figure 2 As shown, the preservative agent cultured with alternating magnetic field and aeration can achieve an OD600 of over 0.056 within 10 days, and fluctuate around 0.09 from 14 to 21 days.

[0037] Example 2 This embodiment provides a method for cultivating preservative-preserving bacteria in circulating water enhanced by intermittent aeration coupled with a magnetic field, comprising the following steps: (1) Microbial culture The compound microbial agent, comprising, by weight, 10 parts Burkholderia, 8 parts Pyridobacterium tumefaciens, 8 parts Plankton, 5 parts Lactobacillus plantarum, 5 parts Saccharomyces cerevisiae, 2 parts Bacillus cereus, and 2 parts Bacillus subtilis, was added to the aerobic reactor. Magnetite was added to the reactor at a dosage of 5 mg / L. Nutrients were added daily, comprising, by weight, 3 parts anhydrous sodium acetate and glucose (C6H2O). 12 2 parts of O6, 2 parts of ammonium citrate, 2 parts of ammonium sulfate ((NH4)2SO4), 2 parts of sodium chloride, 1 part of potassium dihydrogen phosphate (KH2PO4), 1 part of manganese sulfate·7H2O, and 0.5 parts of beef extract.

[0038] The reactor temperature was maintained at 25℃. During the cultivation process, an alternating magnetic field of 5 mT was applied to the reactor, and the growth of microorganisms was stimulated by intermittent aeration. The aeration flow rate was 0.3 L / min, and the aeration frequency was 1 hour of aeration followed by 3 hours of aeration. The culture was carried out continuously for 14 days, and the sludge sediment in the culture medium was collected to obtain the preservative agent.

[0039] (2) Test results During the cultivation period, the change in bacterial concentration over time was determined using the OD600 method. The results showed that the preservative bacterial agent cultivated under the conditions described in this embodiment could achieve an OD600 value of over 0.05 within 14 days, indicating that the bacterial agent could proliferate normally and reach an effective bacterial count under this parameter combination.

[0040] Example 3 This embodiment provides a method for cultivating preservative-preserving bacteria in circulating water enhanced by intermittent aeration coupled with a magnetic field, comprising the following steps: (1) Microbial culture The compound microbial agent is added to the culture container. The compound microbial agent, by weight, comprises: 2 parts Burkholderia, 5 parts *Pyrrosia lingua*, 5 parts *Plantago asiatica*, 1 part *Lactobacillus plantarum*, 1 part *Saccharomyces cerevisiae*, 3 parts *Bacillus cereus*, and 3 parts *Bacillus subtilis*. Nutrients are added to the container. The nutrient solution, by weight, comprises: 1 part anhydrous sodium acetate and 1 part glucose (C6H2O). 12 1 part of O6, 1 part of ammonium citrate, 1 part of ammonium sulfate ((NH4)2SO4), 1 part of sodium chloride, 1 part of potassium dihydrogen phosphate (KH2PO4), 0.5 parts of manganese sulfate·7H2O, and 0.5 parts of beef extract. Magnetite is added at the same time at a dosage of 20 mg / L.

[0041] Maintain the culture temperature at 35℃. During the culture process, apply an alternating magnetic field of 50mT to the culture container and stimulate the growth of microorganisms by intermittent aeration. The aeration flow rate is 0.1L / min, and the aeration frequency is 1 hour of aeration followed by 3 hours of aeration. Culture continuously for 10 days, collect the sludge sediment in the culture medium to obtain the preservative agent for later use.

[0042] (2) Rotary plate corrosion test Test medium: Circulating cooling water from a power plant, with the following water quality parameters: pH=8.3, calcium hardness 5.92 mmol / L, total alkalinity 3.82 mmol / L, Cl... - Concentration 135.30 mg / L.

[0043] Test pads: made of Q235 carbon steel, with dimensions of 42mm×13mm×2mm. Before use, they were degreased with acetone, dehydrated with anhydrous ethanol, dried, and then weighed to an accuracy of 0.1mg.

[0044] Experimental group: Add the preservative agent obtained from step (1) above to the experimental medium, and add 0.5% of the volume of the experimental medium.

[0045] Test conditions: A rotating plate corrosion tester was used. The test temperature was controlled at 42℃±1℃, the rotation speed was 100r / min, and the test cycle was 72 hours. After the test, the plates were removed, corrosion products were cleaned, and after cleaning and drying, they were weighed. The average corrosion rate was calculated using the weight loss method.

[0046] (3) Test results After the experiment, the surface of the trays was observed. The trays in the experimental group with added preservatives maintained their metallic luster, with only minor corrosion marks visible in some areas. The average corrosion rate of the trays was calculated to be 0.0448 ± 0.0007 mm / a. Figure 3 (As shown).

[0047] Example 4 This embodiment provides a method for cultivating and treating a circulating water preservative agent using intermittent aeration coupled with a magnetic field, comprising the following steps: (1) Microbial culture The compound microbial agent, comprising, by weight parts, 6 parts Burkholderia, 6 parts Pyridobacterium tumefaciens, 6 parts Plankton, 3 parts Lactobacillus plantarum, 3 parts Saccharomyces cerevisiae, 2 parts Bacillus cereus, and 2 parts Bacillus subtilis. Magnetochirite is added to the reactor at a concentration of 12 mg / L. Nutrients are added daily, comprising, by weight parts, 2 parts anhydrous sodium acetate and glucose (C6H2O). 12 1.5 parts of O6, 1.5 parts of ammonium citrate, 1.5 parts of ammonium sulfate ((NH4)2SO4), 1.5 parts of sodium chloride, 1 part of potassium dihydrogen phosphate (KH2PO4), 0.8 parts of manganese sulfate·7H2O, and 0.5 parts of beef extract.

[0048] The reactor temperature was maintained at 40℃. During the cultivation process, an alternating magnetic field of 60 mT was applied to the reactor, and the growth of microorganisms was stimulated by intermittent aeration. The aeration flow rate was 0.2 L / min, and the aeration frequency was 1 hour of aeration followed by 3 hours of aeration stoppage. The culture was carried out continuously for 7 days, and the sludge sediment in the culture medium was collected to obtain the preservative agent.

[0049] (2) Corrosion prevention treatment methods and rotating plate corrosion test The antiseptic agent obtained by the above step (1) was added to the circulating cooling water of the power plant for a rotating plate corrosion test.

[0050] Test medium: Circulating cooling water from a power plant, with the following water quality parameters: pH=8.3, calcium hardness 5.92 mmol / L, total alkalinity 3.82 mmol / L, Cl...- Concentration 135.30 mg / L.

[0051] Test pads: made of Q235 carbon steel, with dimensions of 42mm×13mm×2mm. Before use, they were degreased with acetone, dehydrated with anhydrous ethanol, dried, and then weighed to an accuracy of 0.1 mg.

[0052] Experimental group: Add the preservative agent obtained from step (1) above to the experimental medium, with an addition amount of 0.1% of the volume of the experimental medium.

[0053] Test conditions: A rotating plate corrosion tester was used. The test temperature was controlled at 42℃±1℃, the rotation speed was 100r / min, and the test cycle was 72 hours. After the test, the plates were removed, corrosion products were cleaned, and after cleaning and drying, they were weighed. The average corrosion rate was calculated using the weight loss method.

[0054] (3) Test results After the experiment, the surface of the tray was observed. The metallic luster of the tray was basically maintained, with only slight corrosion marks visible in some areas. The average corrosion rate of the tray was calculated to be 0.1245 ± 0.0102 mm / a.

[0055] The above results indicate that adding the preservative agent cultivated in this invention at a dosage of 0.1% to the power plant circulating water system can effectively inhibit the corrosion of Q235 carbon steel.

[0056] Example 5 This embodiment provides a method for cultivating and treating a circulating water preservative agent using intermittent aeration coupled with a magnetic field, comprising the following steps: (1) Microbial culture The compound microbial agent, comprising, by weight parts, 6 parts Burkholderia, 6 parts Pyridobacterium tumefaciens, 6 parts Plankton, 3 parts Lactobacillus plantarum, 3 parts Saccharomyces cerevisiae, 2 parts Bacillus cereus, and 2 parts Bacillus subtilis. Magnetochirite is added to the reactor at a concentration of 12 mg / L. Nutrients are added daily, comprising, by weight parts, 2 parts anhydrous sodium acetate and glucose (C6H2O). 12 1.5 parts of O6, 1.5 parts of ammonium citrate, 1.5 parts of ammonium sulfate ((NH4)2SO4), 1.5 parts of sodium chloride, 1 part of potassium dihydrogen phosphate (KH2PO4), 0.8 parts of manganese sulfate·7H2O, and 0.5 parts of beef extract.

[0057] The reactor temperature was maintained at 40℃. During the cultivation process, an alternating magnetic field of 60 mT was applied to the reactor, and the growth of microorganisms was stimulated by intermittent aeration. The aeration flow rate was 0.2 L / min, and the aeration frequency was 1 hour of aeration followed by 3 hours of aeration stoppage. The culture was carried out continuously for 7 days, and the sludge sediment in the culture medium was collected to obtain the preservative agent.

[0058] (2) Corrosion prevention treatment methods and rotating plate corrosion test The antiseptic agent obtained by the above step (1) was added to the circulating cooling water of the power plant for a rotating plate corrosion test.

[0059] Test medium: Circulating cooling water from a power plant, with the following water quality parameters: pH=8.3, calcium hardness 5.92 mmol / L, total alkalinity 3.82 mmol / L, Cl... - Concentration 135.30 mg / L.

[0060] Test pads: made of Q235 carbon steel, with dimensions of 42mm×13mm×2mm. Before use, they were degreased with acetone, dehydrated with anhydrous ethanol, dried, and then weighed to an accuracy of 0.1 mg.

[0061] Experimental group: The preservative agent obtained by the above step (1) was added to the experimental medium, and the amount added was 1% of the volume of the experimental medium.

[0062] Test conditions: A rotating plate corrosion tester was used. The test temperature was controlled at 42℃±1℃, the rotation speed was 100r / min, and the test cycle was 72 hours. After the test, the plates were removed, corrosion products were cleaned, and after cleaning and drying, they were weighed. The average corrosion rate was calculated using the weight loss method.

[0063] (3) Test results After the experiment, the surface of the plate was observed. The metallic luster of the plate was basically maintained, with only slight corrosion marks visible in some areas. The average corrosion rate of the plate was calculated to be 0.0387 ± 0.0051 mm / a.

[0064] The above results indicate that adding the preservative agent cultivated in this invention at a dosage of 1% to the power plant circulating water system can significantly inhibit the corrosion of Q235 carbon steel.

[0065] Comparative Example 1 This comparative example illustrates the cultivation effect of the compound microbial agent without applying an alternating magnetic field and without intermittent aeration, and is compared with Example 1.

[0066] The same compound bacterial agent, magnetite dosage (20 mg / L), and nutrient type and dosage as in Example 1 were used and added to the aerobic reactor. The reactor temperature was maintained at 35°C. No magnetic field was applied to the reactor during the cultivation process, and no intermittent aeration was used (conventional continuous aeration was employed); cultivation was continuous. The change in bacterial agent content over time was measured using the OD600 method during the cultivation period.

[0067] The results are as follows Figure 2 As shown, the control group without alternating magnetic field and intermittent aeration reached an OD600 value of 0.04 within 14 days, and fluctuated around 0.05 between 14 and 21 days. Compared with Example 1, the experimental group with alternating magnetic field and intermittent aeration reached the same OD600 value (0.04) in 10 days, down from 14 days in the control group. Furthermore, the bacterial agent content (OD600≈0.09) in the later stages of cultivation (14-21 days) was significantly higher than that in the control group (OD600≈0.05), an increase of approximately 80%. This indicates that the addition of alternating magnetic field and intermittent aeration can effectively promote the growth of the compound bacterial agent, significantly shorten the cultivation cycle, and increase the yield of the bacterial agent.

[0068] Comparative Example 2 This comparative example illustrates the corrosion of carbon steel materials under the same water quality conditions without the addition of preservative agents, and is used for comparison with Example 3.

[0069] Test medium: The same power plant circulating cooling water as in Example 3 was used, with the following water quality parameters: pH=8.3, calcium hardness 5.92 mmol / L, total alkalinity 3.82 mmol / L, Cl... - Concentration 135.30 mg / L.

[0070] Test pads: made of Q235 carbon steel, with dimensions of 42mm×13mm×2mm. Before use, they were degreased with acetone, dehydrated with anhydrous ethanol, dried, and then weighed to an accuracy of 0.1mg.

[0071] Control group: No bacterial agents were added to the test medium.

[0072] Test conditions: A rotating plate corrosion tester was used. The test temperature was controlled at 42℃±1℃, the rotation speed was 100r / min, and the test cycle was 72 hours. After the test, the plates were removed, corrosion products were cleaned, and after cleaning and drying, they were weighed. The average corrosion rate was calculated using the weight loss method.

[0073] After the experiment, the surface of the trays was observed. The control group trays were covered with obvious reddish-brown rust products, exhibiting typical uniform corrosion characteristics. The average corrosion rate of the control group trays was 0.8982 ± 0.0183 mm / a. Figure 3(As shown). Compared with Example 3, the corrosion rate of the experimental group with added preservative (addition amount 0.5%) (0.0448±0.0007mm / a) was reduced by 95.09% compared with the control group (0.8982±0.0183mm / a), indicating that the preservative obtained by the present invention has excellent anti-corrosion performance.

[0074] The above content is only for illustrating the technical concept of the present invention and should not be construed as limiting the scope of protection of the present invention. Any modifications made to the technical solution based on the technical concept proposed in this invention shall fall within the scope of protection of the claims of this invention.

Claims

1. A method for cultivating preservative-preserving bacteria in circulating water with intermittent aeration coupled with a magnetic field, characterized in that, Includes the following steps: S1, mix the compound bacterial agent with magnetite or maghemite in the culture medium, and add nutrients to the culture medium; S2, During the cultivation process, an alternating magnetic field is applied to the culture medium, and air is introduced into the culture medium in an intermittent aeration manner, wherein the intermittent aeration is a periodic alternation between aeration and aeration. S3, after cultivation, the sludge precipitate in the culture medium is collected to obtain the intermittent aeration coupled magnetic field enhanced circulating water antiseptic agent.

2. The method for cultivating preservative-preserving bacteria in circulating water with intermittent aeration coupled with a magnetic field enhancement according to claim 1, characterized in that, The compound microbial agent comprises the following components by mass: 2-10 parts Burkholderia, 5-8 parts Pyridobacterium tumefaciens, 5-8 parts Plankton, 1-5 parts Lactobacillus plantarum, 1-5 parts Saccharomyces cerevisiae, 1-3 parts Bacillus cereus and 1-3 parts Bacillus subtilis.

3. The method for cultivating preservative-preserving bacteria in circulating water with intermittent aeration coupled with a magnetic field enhancement according to claim 1, characterized in that, The nutrient solution comprises, by mass, the following components: 1-3 parts anhydrous sodium acetate, 1-2 parts glucose, 1-2 parts ammonium citrate, 1-2 parts ammonium sulfate, 1-2 parts sodium chloride, 1 part potassium dihydrogen phosphate, 0.5-1 part manganese sulfate·7H2O, and 0.5 parts beef extract.

4. The method for cultivating intermittent aeration coupled with a magnetic field to enhance the cultivation of preservative bacteria in circulating water according to claim 1, characterized in that, The amount of magnetite or maghemite added to the culture solution is 5~20 mg / L.

5. The method for cultivating a preservative-preserving agent in circulating water with intermittent aeration coupled with a magnetic field enhancement according to claim 1, characterized in that, The temperature of the culture medium is controlled at 25~40℃ during the culture process.

6. The method for cultivating intermittent aeration coupled with a magnetic field to enhance the cultivation of preservative bacteria in circulating water according to claim 1, characterized in that, The magnetic field strength of the alternating magnetic field is 5~60 mT.

7. The method for cultivating intermittent aeration coupled with a magnetic field to enhance the cultivation of preservative bacteria in circulating water according to claim 1, characterized in that, The intermittent aeration flow rate is 0.1~0.3 L / min, and the aeration frequency is 1 hour of aeration followed by 3 hours of aeration cessation.

8. The method for cultivating a preservative-preserving agent in circulating water with intermittent aeration coupled with a magnetic field enhancement according to claim 1, characterized in that, The culture time is 7 to 14 days.

9. A method for using an intermittent aeration coupled with a magnetic field to enhance the use of a circulating water preservative agent, characterized in that, Includes the following steps: The preservative agent was obtained by culturing using the culturing method described in any one of claims 1 to 8; The preservative agent is added to the circulating water system; The comprehensive index of the preservative agent in the circulating water system is detected. When the comprehensive index is lower than the preset threshold, nutrients, magnetite or maghematite, or preservative agent are added accordingly.

10. The method of using the intermittent aeration coupled magnetic field enhanced circulating water preservative agent according to claim 9, characterized in that, Based on the total volume of the circulating water system, the dosage of the preservative agent is 0.1% to 1%; the preset threshold and replenishment method are as follows: When OD600 < 0.02, add preservative agent; When OD600 ≥ 0.02 and oxygen consumption rate < 15 mg / (g MLVSS·h), supplement with nutrients; When OD600 ≥ 0.02, oxygen consumption rate ≥ 15 mg / (g MLVSS·h), and qPCR detection function of microbial copy number < 10 6 When the volume is in copies / μL, supplement with magnetite or maghemite.

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