Tagatose 6-phosphate epimerase mutants and their use in increasing conversion of d-tagatose

Abstract

This invention discloses a tagatose 6-phosphate epimerase mutant and its application in improving D-tagatose conversion, belonging to the fields of genetic engineering and enzyme engineering technology. The tagatose 6-phosphate epimerase mutant disclosed in this invention exhibits the following mutations based on the amino acid sequence shown in SEQ ID NO.9: N139I-Q192W, Q192W, or N139I. The optimal mutant TPE3 produces a final tagatose concentration of 120.6 g / L, with a relative mass conversion rate of 80.4% to maltodextrin. This invention utilizes a multi-enzyme catalytic route to prepare tagatose, offering advantages such as high substrate utilization, low cost, and high tagatose yield, making it suitable for large-scale tagatose production.

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