Domestication method of long-handled almond tissue culture seedlings

Abstract

The present application relates to long handle almond seedling technology field, specifically to four systems of long handle almond tissue culture seedling domestication. Based on the environmental data detection result, the ventilation temperature control system judges the opening and closing of the ventilation system 1 and 2 and the small bow shed through the condition function, regulates the temperature and the concentration of carbon dioxide, and provides suitable temperature and carbon dioxide for long handle almond tissue culture seedlings. The light supplement control system manages the light supplement system according to the light supplement control formula and the cotton blanket winding controller, and provides suitable light conditions for long handle almond tissue culture seedlings. The water circulation temperature control system takes the condition function as the basis for judgment, realizes temperature increase and decrease through water medium, and avoids long handle almond tissue culture seedlings from suffering from high and low temperature stress. The present application establishes a complete set of simple and operable domestication system for long handle almond tissue culture seedling domestication through automatic detection and management of temperature, humidity, light and carbon dioxide.

Description

Technical Field

[0001] This invention relates to the field of long-stalked almond seedling technology, specifically to a supporting system and method for the domestication of long-stalked almond tissue culture seedlings. Background Technology

[0002] Long-stalked almond ( Prunus pedunculata Pall. (Prunus pedunculata) is an emerging ecological and economical woody oilseed tree species. Due to its drought resistance, cold resistance, and tolerance to poor soil conditions, Pall. has great potential for promotion in ecologically fragile areas. As an excellent woody oilseed tree species, the kernel of Pall. has an oil content as high as 45%-58%, and the content of unsaturated fatty acids in the oil is as high as 98%, ranking first among all edible oils, belonging to high-grade edible oil. Developing Pall. has the advantages of "not competing with grain for land or people for grain," and its kernel, as a source of vegetable oil, can, to a certain extent, reduce my country's dependence on imported oilseeds.

[0003] Currently, artificial cultivation of *Prunus pedunculata* in northern Shaanxi and other regions has reached a considerable scale, but production suffers from unstable yields, low overall quality, and significant alternate bearing (biennial bearing). Existing research is also severely disconnected from production, lacking a robust scientific and technological support system, particularly in areas such as improved variety development, which seriously hinders the application of *Prunus pedunculata* as a woody oilseed tree. Tissue culture is an effective way to rapidly obtain large quantities of asexual seedlings for improved varieties of *Prunus pedunculata*, and it is also an important means to promote the development of the *Prunus pedunculata* industry, urgently needed for germplasm resource research. The acclimatization of *Prunus pedunculata* tissue-cultured seedlings requires stable and suitable temperature and humidity conditions. However, the large diurnal temperature range and high light intensity in the Three-North Region (Northeast, North, and Northwest China) lead to large daily temperature and humidity fluctuations, which are extremely unfavorable for the acclimatization of tissue-cultured seedlings and become a key factor affecting the success of tissue culture seedling cultivation.

[0004] This invention combines the temperature, light, and humidity requirements of long-stalked almond tissue culture seedlings with four systems, including a ventilation and temperature control system, a supplemental lighting control system, a water circulation and temperature control system, and an environmental data monitoring system. It provides a simple and feasible domestication system for long-stalked almonds, which can significantly improve the success rate of domestication and avoid the blindness of domestication management. Summary of the Invention

[0005] The purpose of this invention is to overcome the shortcomings of existing technologies and provide a complete set of supporting systems and acclimatization methods. This provides a simple and feasible acclimatization system for *Prunus pedunculata* tissue culture, improving acclimatization efficiency and avoiding the blind spots in equipment and seedling cultivation. This invention includes four control systems: a ventilation and temperature control system, a supplemental lighting control system, a water circulation and temperature control system, and an environmental data monitoring system.

[0006] The objective of this invention is achieved through the following technical solution: Ventilation and temperature control system Hardware construction for the ventilation and temperature control system. Two automatic control systems for the rolling shutters will be installed in the greenhouse, one for the bottom and one for the top (external, such as...). Figure 1 As shown, there are two systems in the greenhouse (automatic control of the rolling shutter machine controller). The ventilation control points are located at the upper and lower junctions of the greenhouse film. By controlling the ventilation at the bottom and top separately and in combination, the temperature of the greenhouse can be controlled.

[0007] Temperature control and ventilation system management The automatic rolling shutter machines No. 1 and No. 2 of the greenhouse are managed according to the following conditional functions: IF Tm [日平均温度] ≤10℃ and Tm [大棚内温度] ≥26 ℃ "Turn on (Automatic Roller Shutter No. 2)" Tm [日平均温度] ≤10℃ and Tm [大棚内温度] ≤21 ℃ "Turn off (Automatic Roller Shutter No. 2)" 21℃≥Tm [日平均温度] >10℃ and Tm [大棚内温度] ≥ 26 ℃ "Turn on (Automatic Roller Shutter No. 1)" 21℃≥Tm [日平均温度] >10℃ and Tm [大棚内温度] ≤ 20 ℃ "Turn off (Automatic Roller Shutter No. 1)" Tm [日平均温度] >21 ℃ and Tm [大棚内温度] ≥ 26 ℃ "Turn on (automatic roller shutters #1 and #2)" Tm [日平均温度] >21 ℃ and Tm [大棚内温度] ≤ 20 ℃ "Turn off (automatic rolling shutter machines #1 and #2)" End Ventilation management of small arched sheds for domestication inside greenhouses: IF C [二氧化碳浓度] ≤400 PPM and E [光照强度] >1000 lux [Avoid opening ventilation at night] "Open the small arched shed for ventilation" W [小弓棚湿度] ≤85% [If humidity decreases during ventilation, spray humidifiers promptly to increase humidity] "Spray humidification" C [二氧化碳浓度] >490 PPM "Close the small arched shed for ventilation" End supplemental lighting control system The construction of the supplementary lighting control system involves setting up one or two systems based on the light intensity of the supplementary lights and the load-bearing capacity of the power distribution lines. The spacing between each supplementary light is 1-3 meters. Each supplementary lighting system is equipped with an automatic time-controlled switch or an internet-connected remote control switch (e.g., [missing information]). Figure 2 (As shown).

[0008] The greenhouse quilts are rolled up and down by an electric rolling shutter machine (e.g.) Figure 3 The cotton quilts effectively maintain the temperature inside the greenhouse, and the rolling up and unrolling of the quilts also controls the duration and intensity of natural sunlight inside the greenhouse. The time for rolling up the quilts is delayed by 30 minutes to 1 hour based on the local sunrise time, while the time for unrolling them is 30 minutes to 1 hour earlier than the sunset time.

[0009] The supplemental lighting control system manages the duration of supplemental lighting, which is related to the intensity and duration of natural light. If the effective light exposure time reaches or exceeds the time required for long-stalked almonds, supplemental lighting is unnecessary. The effective light exposure time for long-stalked almonds can be determined by... Figure 4 Formula ① shows that the effective light intensity for long-stalked almonds is set above 1400 LUX. Light intensity exceeding 35000 LUX will trigger photo-dormancy. Once the light intensity exceeds the upper limit, appropriate shading or other methods can be used to reduce the light intensity back to the effective range. Supplemental lighting duration T [补光时间] can be Figure 4 Formula ② is used for calculation. After the quilt is laid down, the light intensity inside the shed will drop below 10 LUX, therefore, the supplemental lighting T should be turned on. [开启补光灯时间] Set the time for putting down the quilt, see Figure 4 Formula ③. The time it takes to turn off the fill light is the same as the time it takes to turn on the fill light, T. [补光时间] Later, see Figure 4 Formula ④.

[0010] Water circulation temperature control system Construction of a water circulation temperature control system. This includes automatic control heaters, booster pumps, radiators, piping, and a water tower (see...). Figure 5 This system is commonly used for winter temperature control. Because tissue-cultured seedlings are less susceptible to bacterial contamination and have more readily available labor, acclimatization is often carried out in autumn and winter. However, since *Prunus pedunculata* tissue-cultured seedlings are quite delicate, temperatures below 10℃ can negatively impact their growth. This patent relates to increasing the air temperature by using water as a medium and an electric heater to heat the circulating water. In areas where conditions permit, heat sources such as wood, charcoal, natural gas, sunlight, and geothermal energy can also be used for this purpose.

[0011] The water circulation heating control system is managed by automatically controlling the heater through the following conditional functions.

[0012] IF Tm [大棚内温度] ≤10℃ "Turn on the automatic heater control" [Set the heating water temperature to 50-70℃] Tm [大棚内温度] >15℃ "Turn off the automatic heater control" End Summer temperatures are high, and ventilation alone cannot achieve cooling. The following conditional function is used to manage the water circulation cooling control system for cooling.

[0013] IF Tm [大棚内温度] ≥ 35℃ "Turn on the automatic heater control" [Turn off the heating and use the cold water circulation system in the greenhouse pool]. Tm [大棚内温度] ≤ 30℃ "Turn off the automatic heater control" End Environmental data monitoring system Environmental data monitoring systems collect environmental data, which forms the data foundation for the manual or automated management of the above systems.

[0014] Environmental data acquisition system (see) Figure 6 The system uses probes to collect data in real time, including atmospheric temperature, humidity, carbon dioxide concentration, soil temperature and humidity, light intensity, and soil N, P, and K content. The data is then uploaded to a network data platform using the data collector as a data terminal. Administrators can view the relevant data in real time via mobile phone, tablet, or computer and manage the system using the above-mentioned conditional functions or formulas based on the data information.

[0015] The beneficial effects of this invention are: The present invention provides a highly operable method for the acclimatization of tissue culture seedlings of long-stalked almonds. It offers a simple and operable acclimatization system for tissue culture seedlings. For the acclimatization of tissue culture seedlings of other plants, this invention can be referenced. By combining the characteristics of the corresponding plants and appropriately adjusting the control points, the acclimatization of their tissue culture seedlings can be made controllable, avoiding the blindness of human operation and improving the success rate of acclimatization. Attached Figure Description

[0016] Figure 1 Schematic diagram of the control system for two automatic rolling shutter machines in the greenhouse (bottom ① and top ②) Figure 2 Schematic diagram of greenhouse supplemental lighting and control system Figure 3 Schematic diagram of greenhouse cotton quilt rolling and unrolling machine system Figure 4 supplemental lighting control formula Figure 5 Schematic diagram of water circulation temperature control system Figure 6 Schematic diagram of environmental data monitoring system Detailed Implementation

[0017] The technical solution of the present invention will be further described in detail below with reference to specific embodiments and accompanying drawings, but the scope of protection of the present invention is not limited to the following description. Example

[0018] The supporting system and methods for the domestication of tissue culture seedlings of *Prunus pedunculata* include: A ventilation and temperature control system is used to regulate temperature and carbon dioxide concentration. A supplemental lighting control system that uses a controller to manage the supplemental lighting system; The water circulation temperature control system uses water as a medium to increase and decrease temperature, preventing tissue culture seedlings of long-stalked almonds from suffering from high and low temperature stress. The environmental data monitoring system collects environmental data, providing the data foundation for the automation or manual management of the above systems.

[0019] Specifically, the ventilation and temperature control system includes system hardware construction and management, with two ventilation systems at the bottom and top. The two ventilation systems can be switched on and off in coordination to adapt to ventilation requirements under different conditions. Specifically, it is managed according to the temperature, humidity and carbon dioxide concentration requirements of the long-stalked almond tissue culture seedlings based on conditional functions.

[0020] Specifically, the supplementary lighting control system device and management determine the supplementary lighting time based on the light intensity of the supplementary lighting system and the effective daily light duration in the greenhouse, and carry out supplementary lighting with reference to four formulas, namely formulas ①-④.

[0021] Specifically, the water circulation temperature control system is constructed and managed. This system is an effective measure for adjusting low temperatures in winter and high temperatures in summer. In winter, heating is achieved by heating the water medium with different heat sources and circulating the water; in summer, cooling is achieved by circulating groundwater to reduce the high temperatures. The management reference condition function for long-stalked almonds is also provided.

[0022] Specifically, the environmental data detection system records various environmental data through sensors and utilizes wireless WiFi configuration to collect and detect domestication environment parameters in real time from all directions. These parameters guide the management of the above system, effectively control the domestication environment, and improve the domestication success rate.

[0023] The four systems of this invention were constructed at the tissue culture center of the Ecological Protection and Construction Association in Shenmu City, Shaanxi Province, using a greenhouse at the center.

[0024] For the ventilation and temperature control system, an automatic rolling shutter control system (bottom ventilation system #1) is installed 60cm above the ground. The electric rolling shutter model is 13MD-100-D241. A top control system (top ventilation system #2) is installed 251cm above the ground. Acclimatization is mainly carried out in winter, when the average daily temperature is below 10℃, and ventilation system #1 is activated. A movable small arched shed covered with plastic film is used for moisture retention of the tissue culture seedlings. When the carbon dioxide concentration in the small arched shed drops below 400 PPM and the light intensity reaches above 1000 LUX, the small arched shed is opened for ventilation. After 15 minutes of ventilation, the carbon dioxide concentration can increase to above 490 PPM. The ventilation is then closed, and humidification is achieved by spraying with a mister during this period.

[0025] A supplemental lighting control system was constructed, with a 100W supplemental light installed every 2 meters, 120 cm above the ground. A KG316T timer switch was used to control the on / off times of the supplemental lights. Taking January 9, 2023 as an example, at 9:09 AM, the light intensity reached 2789.9 LUX, exceeding 1000 LUX; from 11:59 AM to 1:49 PM, the light intensity exceeded 35000 LUX (during this period, the intensity was reduced to below 35000 LUX through shading netting); and at 4:09 PM, the blankets were lowered. [长柄扁桃日有效光照需求量] Set to 11h, via Figure 4 Calculate using formulas ①-④, with 5 hours of supplemental lighting, and 16:09 as the starting time.

[0026] The water circulation temperature control system uses a 20 kW electric boiler for water heating (commercial purchase is available), with the on and off temperatures set at 10℃ and 15℃, and is connected to one booster pump, 10 radiators, and one funnel.

[0027] The environmental data monitoring system utilizes the industrial-grade sensing device GS1 (commercially available), connected to temperature sensor probes, carbon dioxide sensor probes, light sensors, humidity sensors, and soil temperature and humidity sensors. It is set to collect relevant data every 15 minutes. Based on this data, tissue culture seedling acclimatization staff manage the acclimatization of the seedlings using the relevant formulas and conditional functions of this invention. This invention can meet the needs of acclimatization of *Prunus pedunculata* tissue culture seedlings, greatly reducing the probability of acclimatization failure due to human error.

[0028] The above description is merely a preferred embodiment of the present invention. It should be understood that the present invention is not limited to the forms disclosed herein and should not be construed as excluding other embodiments. It can be used in various other combinations, modifications, and environments, and can be altered within the scope of the concept described herein through the above teachings or related technologies or knowledge. Modifications and variations made by those skilled in the art that do not depart from the spirit and scope of the present invention should be within the protection scope of the appended claims.

Claims

1. A method for acclimatizing tissue culture seedlings of *Prunus pedunculata*, characterized in that: It includes four control systems: a ventilation and temperature control system, a supplementary lighting control system, a water circulation and temperature control system, and an environmental data monitoring system.

2. The S1 ventilation and temperature control system regulates temperature and carbon dioxide concentration to meet the suitable temperature and carbon dioxide requirements of tissue culture seedlings; The S2 supplemental lighting control system regulates the supplemental lighting system to meet the light requirements of tissue culture seedlings. The S3 water circulation temperature control system uses water as a medium to increase or decrease temperature, preventing tissue culture seedlings of long-stalked almonds from suffering from high or low temperature stress. The S4 environmental data monitoring system collects environmental data to provide a basis for the automation or manual management of the above systems.

3. The method for acclimatizing tissue culture seedlings of *Prunus pedunculata* according to claim 1, characterized in that, The ventilation and temperature control system described in S1 establishes two sets of automatic control rolling shutter control systems for the tissue culture and acclimatization of long-stalked almonds. These systems are divided into bottom and top systems. The ventilation control points are located at the upper and lower junctions of the greenhouse film. The daily average temperatures of 10℃ and 21℃, as well as the greenhouse temperatures of 20℃, 21℃, and 26℃, are the temperature control nodes. The carbon dioxide concentration of 400PPM and the light intensity of 1000lux are used to avoid ventilation at night. In this way, the acclimatization temperature is regulated by controlling the ventilation at the bottom and top separately and in combination.

4. The method for acclimatizing tissue culture seedlings of *Prunus pedunculata* according to claim 1, characterized in that, The supplementary lighting control system described in S2 consists of one or two supplementary lighting systems, with each supplementary light spaced 1-3 meters apart. Each supplementary lighting system is equipped with an automatic time control switch or an internet remote control switch. The light intensity is 1400 LUX as the supplementary lighting control node and 35000 LUX as the light dormancy shading control node.

5. The method for acclimatizing tissue culture seedlings of *Prunus pedunculata* according to claim 1, characterized in that, The water circulation temperature control system described in S3 includes an automatic control heater, a booster pump, radiators, pipes, and a water tower. This system is used for winter heating control and summer cooling control: 10℃ and 15℃ are used as the switching temperature nodes for winter heating (circulating water temperature set to 50-70℃), and 35℃ and 30℃ are used as the switching temperature nodes for summer cooling (utilizing cold water circulation from an indoor pool).

6. The method for acclimatizing tissue culture seedlings of *Prunus pedunculata* according to claim 4, characterized in that, The environmental data detection system described in S4 collects data in real time through probes for atmospheric temperature, atmospheric humidity, atmospheric carbon dioxide concentration, soil temperature and humidity, light intensity, and soil N, P, K, etc., providing basic data for the manual or automated management of the above systems.

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