Method for promoting the growth and development of sorghum and application thereof

Abstract

The application belongs to the technical field of microbial agent application, and relates to a method for promoting the growth and development of sorghum, comprising the following steps: inoculating a Defluviicoccus vanus strain into a culture medium, carrying out constant-temperature oscillation culture, and obtaining activated bacterial liquid; centrifugally collecting bacterial body precipitates from the activated bacterial liquid, washing the bacterial body precipitates with sterile water, resuspending the bacterial body precipitates in sterile water, and preparing a uniform bacterial strain suspension; and at the three-leaf stage of the sorghum seedlings, the prepared bacterial suspension is used for root irrigation of the sorghum seedlings. The biological activity of the Defluviicoccus vanus strain is recovered through strain activation culture, a high-purity uniform bacterial suspension is prepared through centrifugal washing and resuspension in sterile water, root irrigation is carried out at the key window period of root system development of the sorghum at the three-leaf stage, the functional strain is accurately attached to the root system and stably planted in the rhizosphere microecological environment, and the nutrient regulation effect is continuously exerted, so that the growth defects of the sorghum seedlings, such as poor root system development, slender stems and weak growth, are effectively solved.

Description

Technical Field

[0001] This invention belongs to the field of microbial inoculant application technology, and relates to a method for promoting the growth and development of sorghum and its application. Background Technology

[0002] Red tassel sorghum is a glutinous sorghum variety exclusive to Maotai Town, Renhuai, Guizhou Province, and is the only core raw material for brewing sauce-flavored baijiu. This variety has small grains, thick skin, amylopectin content exceeding 90%, and moderate tannin content, making it suitable for the nine-round steaming and fermentation process of Kunsha. It is a key basic raw material determining the flavor and production capacity of sauce-flavored baijiu. However, due to factors such as low soil organic matter, spring drought, weak seedling growth, and thin, easily lodging stems, the yield of red tassel sorghum under traditional planting methods is low, resulting in a long-standing raw material supply gap and hindering the large-scale and stable development of the sauce-flavored baijiu industry. Currently, the industry mainly relies on two approaches to increase red tassel sorghum yield: increasing the application of chemical fertilizers and using conventional microbial agents, but both have significant shortcomings.

[0003] Existing microbial agents for promoting sorghum growth are mostly isolated from farmland soil and crop rhizosphere, primarily consisting of conventional rhizosphere growth-promoting bacteria (PGPRs) such as Bacillus, Pseudomonas, and endophytic fungi. While these strains possess basic growth-promoting functions such as phosphate solubilization and growth promoter production, their adaptability is poor, resulting in limited growth enhancement for sorghum. Most agents only achieve slight yield increases and fail to simultaneously promote root development and stem thickening. Furthermore, commercially available compound microbial agents have complex compositions, with antagonistic effects between strains, and some bacterial groups even inhibiting sorghum seedling growth. Summary of the Invention

[0004] The purpose of this invention is to provide a method for promoting the growth and development of sorghum and its application, addressing the technical problems existing in the cultivation of red-tasseled sorghum, such as weak seedling growth, poor root development, thin stems, limited yield increase, and poor compatibility and unstable growth-promoting effects of conventional growth-promoting microorganisms with the rhizosphere of red-tasseled sorghum. By precisely irrigating the roots with the *Defluviicoccus vanus* strain during the seedling stage, the strain is stably established in the rhizosphere microenvironment of red-tasseled sorghum without altering the original planting pattern. Through regulating the metabolism of carbon, nitrogen, phosphorus, and sulfur nutrients and the synthesis of secondary metabolites in the rhizosphere, the method effectively improves the growth status of sorghum seedlings, promotes root elongation and stem thickening, and significantly increases the biomass and final yield of red-tasseled sorghum. The process is simple, stable, and reproducible, effectively improving the quality and yield of red-tasseled sorghum, and has promising application prospects in the field of green cultivation of sorghum for sauce-flavored liquor production.

[0005] Based on this, according to a first aspect of the present invention, the present invention provides a method for promoting the growth and development of sorghum, characterized by comprising the following steps: Strain activation: Defluviicoccus vanus strain was inoculated into the culture medium and cultured under constant temperature and shaking to obtain activated bacterial solution; Preparation of bacterial suspension: Centrifuge the activated bacterial suspension to collect the bacterial precipitate, wash with sterile water, resuspend in sterile water, and prepare a uniform bacterial suspension. Root irrigation treatment: At the three-leaf stage of sorghum seedlings, the prepared bacterial suspension is applied to the roots of the sorghum seedlings.

[0006] In some embodiments of the present invention, in the strain activation step, the culture medium is MA liquid culture medium, and the shaking culture temperature is 25~35℃, the rotation speed is 160~200 r / min, and the time is 15~20h.

[0007] In some embodiments of the present invention, in the bacterial culture preparation step, the centrifugation speed is 7000~9000 r / min and the time is 3~10 min.

[0008] In some embodiments of the present invention, in the root irrigation treatment step, the amount of live bacteria applied in the bacterial suspension ranges from 10. 7 ~10 9 CFU / g soil; preferably, the viable bacteria dosage of the bacterial suspension is 10. 8 CFU / g soil.

[0009] In some embodiments of the present invention, the Defluviicoccus vanus strain genome carries functional genes related to nitrogen metabolism, phosphorus metabolism, carbon metabolism, sulfur metabolism, and secondary metabolite biosynthesis, which promote sorghum growth and development by regulating rhizosphere nutrient cycling.

[0010] In some embodiments of the present invention, promoting sorghum growth and development includes promoting sorghum root elongation, increasing stem diameter, and / or increasing sorghum yield.

[0011] In some embodiments of the present invention, the sorghum is red-tasseled sorghum.

[0012] In some embodiments of the present invention, the yield of sorghum per mu is increased by 20-30%; the stem diameter is increased by 20-30%; and the root length is increased by 10-15%.

[0013] In some embodiments of the present invention, in the root irrigation treatment step, the sorghum seeds of the sorghum seedlings are soaked in a 2-5% hydrogen peroxide solution for 15-25 minutes, and then rinsed with sterile water 3-5 times.

[0014] Secondly, this application also provides a method for promoting the growth and development of sorghum, applied to the cultivation of red tassel sorghum, a raw material for Baijiu brewing.

[0015] Compared with the prior art, the present invention has at least the following beneficial effects: The biological activity of the Defluviicoccus vanus strain was restored through strain activation culture. A high-purity, homogeneous bacterial suspension was prepared by centrifugation, washing, and sterile water resuspending. Root irrigation was then performed during the critical window of root development at the three-leaf stage of sorghum. This allowed the functional strain to precisely attach to the roots, stably establish itself in the rhizosphere microecological environment, and continuously exert its nutrient regulation function. This effectively solved the growth defects of poor root development, thin stems, and weak growth in the seedling stage of red tassel sorghum, significantly improved the plant's nutrient absorption capacity and growth vigor, and achieved improved sorghum growth and stable yield increase. Attached Figure Description

[0016] Figure 1 The image shows the structural equation model (SEM) analysis results of the influence of topography and microbial community on the yield of red sorghum in this application. In the image, the dashed line represents a relationship with P>0.1, the solid line represents a relationship with P<0.1, the thick solid line represents a significant relationship with P<0.05, red represents a positive correlation, green represents a negative correlation, and the values ​​on the lines are standardized path coefficients. Figure 2 This is a bar chart comparing the stem diameter and root length of *Defluviicoccus vanus* strain inoculated with *Sorghum sorghum* seedlings. The blank column represents the water control group (CK), and the striped column represents the strain treatment group. The error bars on the columns represent the standard deviation of replicate samples. * indicates a significant difference (P < 0.05), ** indicates an extremely significant difference (P < 0.01), and ns indicates no significant difference between groups. Figure 3 This is a bar chart comparing the stem diameter and root length of *Sorghum sorghum* seedlings after inoculation with strain AG11 of this application; the blank column represents the water control group (CK), and the striped column represents the strain treatment group; the error bars on the columns represent the standard deviation of replicate samples; * represents P<0.05, ** represents P<0.01, and ns represents no significant difference between groups. Figure 4 This is a bar chart comparing the yield per mu of *Sorghum sibirica* in the field according to this application; the vertical axis represents the yield per mu, CK is the water control and Defluviicoccus is the root irrigation treatment group of the target strain; the error bars on the column represent the fluctuation range of the yield measured in different experimental plots; the ** indicates that the difference in yield between the two groups is extremely significant (P<0.01). Detailed Implementation

[0017] The following specific embodiments further illustrate the technical solution of the present invention. These specific embodiments do not represent a limitation on the scope of protection of the present invention. Non-essential modifications and adjustments made by others based on the concept of the present invention still fall within the scope of protection of the present invention.

[0018] As described in the background section, current microbial agents for promoting sorghum growth have limited growth-promoting effects, making it difficult to simultaneously promote root development and stem thickening. Compound microbial agents have complex components, and there are antagonistic effects between strains. Some microbial groups may even inhibit the growth of sorghum seedlings, making it impossible to stably achieve improved quality and increased yield.

[0019] To identify microbial strains that promote sorghum growth and develop microbial fertilizer formulations, technicians conducted large-sample soil microbial amplicon sequencing on organically cultivated red sorghum plots in the mountainous areas of Guizhou. Simultaneously, they used field-measured yield data. Microbial abundance, topographic environmental parameters, and yield data were incorporated into a multi-factor mixed-effects model and a structural equation model (SEM) for modeling analysis. This investigation explored the impacts of altitude, slope, bacterial α-diversity, fungal α-diversity, microbial community aggregation, and abundance of various microbial genera on sorghum yield.

[0020] Modeling results are as follows Figure 1 As shown, the abundance of Defluviicoccus microorganisms is significantly positively correlated with the yield of red tassel sorghum, while AG11-type bacteria have a negative impact on yield. Therefore, Defluviicoccus vanus was identified as the target candidate growth-promoting bacteria, and AG11-type strains were used as negative control strains for subsequent validation experiments.

[0021] *Defluviicoccus vanus*, a glycogen-accumulating bacterium belonging to the Rhodospirilluceae family, is primarily studied and applied in wastewater treatment. Existing literature confirms that this strain can absorb short-chain fatty acids under alternating anaerobic / aerobic conditions, storing glycogen and polyhydroxyalkanoates intracellularly, making it a dominant functional bacterium in activated sludge nitrogen and phosphorus removal systems. Genomic analysis shows that *Defluviicoccus vanus* carries complete genes for nitrogen, phosphorus, carbon, sulfur metabolism, and secondary metabolite biosynthesis. However, current global research focuses solely on its sludge metabolic competition mechanisms, and no literature has yet disclosed its application in the rhizosphere of crops.

[0022] Sequencing alone confirmed the correlation between sorghum field microbial communities and yield, but it was impossible to screen and verify functional strains with stable growth-promoting effects. There was a lack of a complete inoculation process and field validation system for applying Defluviicoccus vanus from sludge to red tassel sorghum for sauce-flavored sorghum, and it was not confirmed that this strain could simultaneously improve root and stem traits and achieve significant yield increases.

[0023] To address the aforementioned problems, this application provides a method for promoting the growth and development of sorghum.

[0024] The Defluviicoccus vanus strain used in the embodiments of this invention was purchased from the website http: / / lpsn.dsmz.de / .

[0025] A method for promoting the growth and development of sorghum includes the following steps: Strain activation: Defluviicoccus vanus strain was inoculated into the culture medium and cultured under constant temperature and shaking to obtain activated bacterial solution; Preparation of bacterial suspension: Centrifuge the activated bacterial suspension to collect the bacterial precipitate, wash with sterile water, resuspend in sterile water, and prepare a uniform bacterial suspension. Root irrigation treatment: At the three-leaf stage of sorghum seedlings, the prepared bacterial suspension is applied to the roots of the sorghum seedlings.

[0026] The biological activity of the Defluviicoccus vanus strain was restored through strain activation culture. A high-purity, homogeneous bacterial suspension was then prepared by centrifugation, washing, and sterile water resuspending. This eliminated the adverse effects of residual culture medium, metabolic impurities, and interference from other bacteria on rhizosphere colonization. Finally, root irrigation was precisely performed during the critical window of root development at the three-leaf stage of sorghum. This allowed the functional strain to accurately attach to the roots, stably colonize in the rhizosphere microecological environment, and continuously exert its nutrient regulation function. This effectively solved the growth defects of poor root development, thin stems, and weak growth in the seedling stage of red tassel sorghum, significantly improved the plant's nutrient absorption capacity and growth vigor, and ultimately achieved improved sorghum growth and stable yield increase.

[0027] Furthermore, in the strain activation step, the culture medium is MA liquid medium, the shaking culture temperature is 25~35℃, the rotation speed is 160~200 r / min, and the time is 15~20h.

[0028] MA liquid medium is cultured at 25–35℃ and 160–200 r / min to ensure stable proliferation and maintain high biological activity of the *Defluviicoccus vanus* strain, providing a fundamental prerequisite for subsequent rhizosphere colonization and growth promotion of sorghum. For example, the shaking culture temperature can be 25℃, 28℃, 30℃, 32℃, 35℃, or any combination thereof; the shaking speed can be 160 r / min, 170 r / min, 180 r / min, 190 r / min, 200 r / min, or any combination thereof; and the time can be 15h, 16h, 17h, 18h, 19h, 20h, or any combination thereof. Below 25℃, the strain's metabolic rate decreases significantly, proliferation is slow, viable cell accumulation is insufficient, and cell activity is weak. Above 35℃, protein denaturation and cell aging may occur, leading to a significant decrease in the number of effective viable cells. When the rotation speed is below 160 r / min, the dissolved oxygen in the culture medium is insufficient, limiting the proliferation of the strain and resulting in uneven growth. When the rotation speed is above 200 r / min, excessive shear force will damage the bacterial cells and reduce the biological activity of the strain. When the culture time is less than 15 h, the strain has not reached the logarithmic growth phase, and the viable cell concentration is not up to standard. When the culture time is longer than 20 h, the strain enters the death phase, metabolic waste accumulates, and a large number of bacterial cells are inactivated, making it impossible to guarantee the stability of subsequent growth promotion effects.

[0029] Furthermore, in the bacterial culture preparation step, the centrifugation speed is 7000~9000 r / min, and the time is 3~10 min.

[0030] Centrifugation parameters of 7000-9000 r / min and 3-10 min can effectively enrich intact active bacterial cells, remove waste culture medium and metabolic impurities, and obtain a pure and homogeneous bacterial suspension, avoiding interference from impurities with rhizosphere colonization. For example, the centrifugation speed can be 7000 r / min, 7500 r / min, 8000 r / min, 8500 r / min, 9000 r / min, or any combination thereof, and the centrifugation time can be 3 min, 5 min, 7 min, 10 min, or any combination thereof. When the speed is below 7000 r / min and the time is less than 3 min, the centrifugal force is insufficient, the bacterial cells do not precipitate completely, and a large number of viable bacteria are lost with the supernatant, resulting in a low effective viable bacteria concentration in the bacterial suspension. When the speed is above 9000 r / min and the time is longer than 10 min, the excessive centrifugation pressure will crush and damage the bacterial cells, causing the strain to become inactive and significantly reducing the number of effective viable bacteria.

[0031] Furthermore, in the root drenching treatment step, the amount of live bacteria applied in the bacterial suspension ranges from 10... 7 ~10 9 CFU / g soil; viable bacterial suspension dosage was 10. 8CFU / g soil.

[0032] 10 7 ~10 9 The CFU / g soil application range of viable bacteria can ensure that the strains stably form a dominant functional flora in the sorghum rhizosphere, continuously regulate the rhizosphere nutrient cycle, and achieve efficient growth promotion and yield increase. Among them, 10 8 The optimal application concentration is CFU / g soil. For example, the amount of viable bacteria applied can be 10. 7 CFU / g soil, 5×10 7 CFU / g soil, 10 8 CFU / g soil, 5×10 8 CFU / g soil, 10 9 The range is CFU / g soil or any combination thereof. Concentration below 10 7 When the concentration of CFU / g soil is too high, the number of rhizosphere colonized bacterial strains is too small to compete with native soil microorganisms, making it impossible to form a dominant bacterial community and thus losing its growth-promoting and yield-increasing functions; when the concentration is higher than 10... 9 When the concentration of CFU / g soil is high, competition within the strain population intensifies, leading to redundant nutrient consumption. This not only fails to improve the growth-promoting effect but also significantly increases the cost of strain cultivation and application.

[0033] Furthermore, the Defluviicoccus vanus strain genome carries functional genes related to nitrogen metabolism, phosphorus metabolism, carbon metabolism, sulfur metabolism, and secondary metabolite biosynthesis, which promote sorghum growth and development by regulating rhizosphere nutrient cycling.

[0034] The Defluviicoccus vanus strain has a unique growth-promoting gene mechanism that distinguishes it from conventional soil growth-promoting bacteria.

[0035] Furthermore, promoting sorghum growth and development includes promoting root elongation, increasing stem diameter, and / or increasing sorghum yield.

[0036] Furthermore, the sorghum is red-tasseled sorghum.

[0037] Furthermore, the yield of sorghum per mu increased by 20-30%; the stem diameter increased by 20-30%; and the root length increased by 10-15%.

[0038] Furthermore, in the root irrigation treatment step, the sorghum seeds of the sorghum seedlings are soaked in a 2-5% hydrogen peroxide solution for 15-25 minutes, and then rinsed with sterile water 3-5 times.

[0039] Soaking seeds in a 2-5% hydrogen peroxide solution for 15-25 minutes can effectively remove surface bacteria, reduce competition from other bacteria, and improve the colonization success rate and repeatability of the target strain. For example, the hydrogen peroxide concentration can be 2%, 3%, 4%, 5%, or any combination thereof, and the soaking time can be 15 minutes, 18 minutes, 20 minutes, 22 minutes, 25 minutes, or any combination thereof. When the hydrogen peroxide concentration is below 2% and the soaking time is less than 15 minutes, the seed surface is not thoroughly sterilized, and residual bacteria will compete for rhizosphere niches, inhibiting the colonization of the target strain. When the hydrogen peroxide concentration is above 5% and the soaking time is longer than 25 minutes, the strong oxidizing effect will damage the embryo, inhibit seed germination, reduce emergence rate, and is detrimental to sorghum seedling growth.

[0040] Furthermore, this application also provides a method for promoting the growth and development of sorghum, applied to the cultivation of red tassel sorghum, a raw material for Baijiu brewing.

[0041] Example 1: Experiment on the promotion of growth and development of red sorghum by Defluviicoccus vanus I. Preparation of bacterial suspension 1. Activation of bacterial strains: Standard strain Defluviicoccus vanus was purchased and inoculated into MA liquid medium. AG11 Gemmatimonadaceae strain was inoculated into LB liquid medium and cultured at 30℃ and 180 rpm for 16 h to obtain activated bacterial solution. AG11 strain belongs to Gemmatimonadaceae and its main functions include participating in soil organic carbon mineralization and influencing soil microbial community composition. It served as a negative control in this experiment. 2. Bacterial cell collection: Under aseptic conditions, transfer the activated bacterial solution into a centrifuge tube, centrifuge at 8000 r / min for 5 min, discard the supernatant culture medium, and collect the bacterial cell precipitate at the bottom; 3. Preparation of bacterial suspension: Wash the bacterial precipitate twice with sterile pure water to remove culture medium residue, then resuspend the bacterial cells with sterile water, perform serial dilution plate counting, and prepare Defluviicoccus vanus to the target viable cell concentration for later use. Prepare AG1 Bacillus strain to the same viable cell concentration as Defluviicoccus vanus for later use.

[0042] II. Comparative Experiment on Promoting Growth in Potted Plants 1. Seed disinfection: Red tassel sorghum seeds are soaked in 3% hydrogen peroxide solution for 20 minutes for surface disinfection, rinsed several times with sterile water to remove residual hydrogen peroxide, and then dried for later use. 2. Potted planting: Fill each pot with 250g of sterilized nutrient soil, sow 1 sterilized seed in each pot, and cultivate with conventional water and fertilizer management until the three-leaf stage; 3. Root irrigation treatment: Each group has 4 biological replicates. Soil moisture content is maintained at 80% of field capacity. When the red sorghum plants reach the three-leaf stage, inoculate with the bacterial suspension. Experimental group: Defluviicoccus vanus bacterial suspension at 10 8 Water the roots of sorghum seedlings with CFU / g soil. Blank control group CK: Irrigated with an equal volume of sterile water; Negative control group: Root irrigation with a bacterial suspension of AG1 strain with the same live bacteria concentration; 4. After 20 days of cultivation in a uniform greenhouse environment, the sorghum stem diameter and root length of each group were measured, and statistical difference analysis was conducted using SPSS software.

[0043] III. The results of the pot experiment are shown in Table 1 and Figures 2-3 As shown: Table 1. Root length and stem diameter of red sorghum seedlings

[0044] Note: * indicates a significant difference compared to the blank control group (P<0.05), ** indicates an extremely significant difference compared to the blank control group (P<0.01); no marker indicates no statistically significant difference between groups.

[0045] The average stem diameter of the blank control group (CK) was 3.42 mm and the average root length was 20.97 cm; the average stem diameter of the Defluviicoccus vanus treatment group was 4.51 mm and the average root length was 23.38 cm. The stem diameter and root length were significantly better than those of the blank control group (P=0.050 and P=0.008, respectively). The two growth indicators were significantly better than those of the blank control group. The average stem diameter and root length of the AG1 treatment group were slightly lower than those of the CK group, with no significant growth-promoting effect and a slight growth-inhibiting effect.

[0046] Example 2: Field plot yield test In a standardized sorghum planting field in Maotai Town, Guizhou Province, three equal experimental plots were divided, with identical soil, altitude, light, and irrigation management conditions. These plots included a control (CK) area, a Defluviicoccus vanus treatment area, and an AG1 control area. Once the sorghum seedlings reached the three-leaf stage, Defluviicoccus vanus bacterial suspension was applied at a concentration of 10... 8 CFU / g soil root irrigation; CK area was irrigated with the same amount of water, and AG1 area was treated with the same concentration of AG1 bacterial suspension prepared in Example 1; after the sorghum was fully mature, it was harvested and weighed separately in different areas, and the yield per unit area was calculated and significance test was carried out.

[0047] Yields were measured in experimental fields inoculated with *Defluviicoccus vanus* and in a control group (CK) of clean water without any strain. The results are as follows: Figure 4 As shown, the yield of the control group (CK) was 538.61 kg / mu, while the yield of the Defluviicoccus vanus treatment group was 676.58 kg / mu, with a highly significant difference between the two groups (P<0.01), representing a yield increase of 25.6%. In contrast, the yield of red sorghum inoculated with strain AG11 was significantly lower than that of the control group (CK). This demonstrates that Defluviicoccus vanus has a significant effect on promoting the growth and development of red sorghum and can be used to increase the yield and income of red sorghum.

[0048] Example 3: Defluviicoccus vanus genome function verification assay The genome sequences of multiple Defluviicoccus strains were retrieved from the NCBI database, and KEGG metabolic pathway annotation analysis was performed. The results are shown in Table 2. Table 2. KEGG functional analysis results of Defluviicoccus genomes from NCBI.

[0049] Note: + indicates that the genome contains functional genes for this pathway.

[0050] All tested strains carried key functional genes related to nitrogen metabolism, phosphorus metabolism, carbon metabolism, sulfur metabolism, and the biosynthesis of secondary metabolites in their genomes. This demonstrates that after entering the rhizosphere of sorghum, these strains can drive soil nutrient cycling, improve the rhizosphere microenvironment, enhance the nutrient absorption capacity of sorghum roots, promote root development and stem thickening, and ultimately increase yield.

[0051] It is understood that the present invention has been described through some embodiments, and those skilled in the art will recognize that various changes or equivalent substitutions can be made to these features and embodiments without departing from the spirit and scope of the invention. Furthermore, under the teachings of the present invention, these features and embodiments can be modified to adapt to specific situations and materials without departing from the spirit and scope of the invention. Therefore, the present invention is not limited to the specific embodiments disclosed herein, and all embodiments falling within the scope of the claims of this application are within the protection scope of the present invention.

Claims

1. A method for promoting the growth and development of sorghum, characterized in that, Includes the following steps: Strain activation: Defluviicoccus vanus strain was inoculated into the culture medium and cultured under constant temperature and shaking to obtain activated bacterial solution; Preparation of bacterial suspension: Centrifuge the activated bacterial suspension to collect the bacterial precipitate, wash with sterile water, resuspend in sterile water, and prepare a uniform bacterial suspension. Root irrigation treatment: At the three-leaf stage of sorghum seedlings, the prepared bacterial suspension is applied to the roots of the sorghum seedlings.

2. The method for promoting sorghum growth and development according to claim 1, characterized in that, In the strain activation step, the culture medium is MA liquid culture medium, and the shaking culture temperature is 25~35℃, the rotation speed is 160~200 r / min, and the time is 15~20h.

3. The method for promoting sorghum growth and development according to claim 1, characterized in that, In the bacterial culture preparation step, the centrifugation speed is 7000~9000 r / min and the time is 3~10 min.

4. The method for promoting sorghum growth and development according to claim 1, characterized in that, In the root drenching treatment step, the amount of live bacteria applied in the bacterial suspension ranges from 10. 7 ~10 9 CFU / g soil; preferably, the viable bacteria dosage of the bacterial suspension is 10. 8 CFU / g soil.

5. The method for promoting the growth and development of sorghum according to claim 1, characterized in that, The Defluviicoccusvanus strain carries functional genes related to nitrogen metabolism, phosphorus metabolism, carbon metabolism, sulfur metabolism, and the biosynthesis of secondary metabolites in its genome, which promotes sorghum growth and development by regulating rhizosphere nutrient cycling.

6. The method for promoting the growth and development of sorghum according to any one of claims 1 to 5, characterized in that, The promotion of sorghum growth and development includes promoting root elongation, increasing stem diameter, and / or increasing sorghum yield.

7. The method for promoting sorghum growth and development according to claim 6, characterized in that, The sorghum in question is red-tasseled sorghum.

8. The method for promoting the growth and development of sorghum according to claim 6, characterized in that, The yield of sorghum per mu increased by 20-30%; stem diameter increased by 20-30%; and root length increased by 10-15%.

9. The method for promoting the growth and development of sorghum according to claim 6, characterized in that, In the root irrigation treatment step, the sorghum seeds of the sorghum seedlings are soaked in a 2-5% hydrogen peroxide solution for 15-25 minutes, and then rinsed with sterile water 3-5 times.

10. The application of the method for promoting the growth and development of sorghum as described in any one of claims 1 to 9 in the cultivation of red tassel sorghum, a raw material for Baijiu brewing.

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