Use of 2,4-di-tert-butylphenol as an adult attractant for the melon fly

Abstract

The application discloses application of 2,4-di-tert-butyl phenol as an adult attractant of leucophenga quadrimaculata. The application also discloses an adult attractant of leucophenga quadrimaculata, wherein an effective component of the adult attractant is 2,4-di-tert-butyl phenol, and the concentration of the 2,4-di-tert-butyl phenol in the attractant is 0.01 mu g / mu L-100 mu g / mu L. The application further provides an attractant core of leucophenga quadrimaculata, wherein the attractant core is loaded with the adult attractant of leucophenga quadrimaculata. EAG determination results show that the 2,4-di-tert-butyl phenol can induce the female and male adults of leucophenga quadrimaculata to produce an antennal electric potential response in a concentration range of 0.01-100 mu g / mu L. The 2,4-di-tert-butyl phenol belongs to a commercial chemical raw material, and is convenient for large-scale processing and preparation of the attractant core of leucophenga quadrimaculata. Field test proves that the attractant core containing the 2,4-di-tert-butyl phenol can effectively attract the female and male adults of leucophenga quadrimaculata in a chenopodium field.

Description

Technical Field

[0001] This invention belongs to the field of green pest control technology in agriculture, specifically involving the application of 2,4-di-tert-butylphenol as an attractant for adult leafhoppers. Background Technology

[0002] Quinoa (Chenopodium quinoa Willd.) is nutritionally complete and possesses high edible and economic value. With the adjustment of the dietary structure of domestic residents, the scale of artificial quinoa cultivation in my country continues to expand. The leaf beetle (Tetanopssintenisi Becker), commonly known as the quinoa root maggot, has become a significant underground pest damaging quinoa in quinoa-growing regions such as Xinjiang, Shanxi, Beijing, and Inner Mongolia. The larvae of this pest congregate underground, feeding on the quinoa roots. Affected plants exhibit yellowing and wilting of the above-ground parts, and in severe cases, the entire plant dies, resulting in significant yield losses in the field.

[0003] Because the larvae of the leafminer's fly hide underground and cause damage, conventional pesticides have difficulty reaching the target insect. Long-term reliance on chemical pesticides for control can easily induce pesticide resistance in the pest and also pose a risk of pesticide residues in the environment. Currently, there are no effective population monitoring and early warning technologies for leafminer's fly; existing publicly available research, both domestic and international, lacks reports on leafminer's pheromones, and the availability of attractant substances suitable for monitoring, trapping, and controlling this pest remains a blank.

[0004] Insect pheromones function through insect chemical communication mechanisms, possessing characteristics such as small dosage, high species specificity, good environmental compatibility, and safety to natural enemies. They are an important technical approach for green pest control and population dynamic monitoring. Therefore, developing pheromone attractants for the leafhopper fly (Leymus chinensis) and combining them with field trapping devices to attract adult flies has practical research and application value for conducting population monitoring and green pest control, enriching the quinoa pest control technology system, and reducing the pressure of chemical pesticide application. Summary of the Invention

[0005] The purpose of this invention is to overcome the shortcomings of the existing technology and provide the application of 2,4-di-tert-butylphenol as an attractant for adult leafhoppers.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: The application of 2,4-di-tert-butylphenol as an attractant for adult leafhoppers, wherein the concentration of 2,4-di-tert-butylphenol in the attractant is 0.01 μg / μL-100 μg / μL.

[0007] Furthermore, the concentration of 2,4-di-tert-butylphenol in the attractant is 10 μg / μL.

[0008] The present invention also provides an attractant for adult leafhoppers of the leafhopper, wherein the active ingredient of the attractant is 2,4-di-tert-butylphenol, and the concentration of 2,4-di-tert-butylphenol in the attractant is 0.01 μg / μL-100 μg / μL.

[0009] Furthermore, the concentration of 2,4-di-tert-butylphenol is 10 μg / μL.

[0010] Furthermore, the attractant also contains the organic solvent n-hexane.

[0011] The present invention also provides a leafhopper attractant for *S. serratus*, wherein the leafhopper attractant is loaded with the aforementioned adult leafhopper attractant.

[0012] The beneficial effects of this invention are as follows: EAG assay results show that 2,4-di-tert-butylphenol can induce antennal potential responses in both male and female adult *Leymus chinensis* within a concentration range of 0.01-100 μg / μL. 2,4-di-tert-butylphenol is a commercially available chemical raw material, characterized by chemical stability, easy solubility in common organic solvents, and a moderate volatility rate, making it suitable for large-scale processing and preparation of *Leymus chinensis* lures. Field trials have confirmed that lures containing 2,4-di-tert-butylphenol can effectively attract both male and female adult *Leymus chinensis* in quinoa fields. Attached Figure Description

[0013] Figure 1 The images show the GC-EAD analysis of the gonadal extracts of adult leafminer's vegetative leaflets; where a is the GC-EAD diagram of the male gonadal extract; b is the GC-EAD diagram of the female gonadal extract; c is the GC-EAD diagram of compound III; and d is the GC-EAD diagram of compound IV. Figure 2 The mass spectrometry identification of 2,4-di-tert-butylphenol is shown below; where a is the measured mass spectrum of compound II in the female gonad extract; b is the mass spectrum of 2,4-di-tert-butylphenol standard; and c is a mirror image comparison of the mass spectra of the sample and the standard. Figure 3 The GC-MS total ion chromatogram of the gonadal extract of adult leafminer's serpentine fly is shown. Figure 4 The diagram shows the relative EAG response of different concentrations of 2,4-di-tert-butylphenol to adult leafhoppers; where a is the relative EAG response of 2,4-di-tert-butylphenol to male leafhoppers and b is the relative EAG response of 2,4-di-tert-butylphenol to female leafhoppers. Figure 5 EAG response spectra of adult *Leymus chinensis* to 2,4-di-tert-butylphenol; where a is the EAG response spectra of male adult *Leymus chinensis* to 2,4-di-tert-butylphenol; and b is the EAG response spectra of female adult *Leymus chinensis* to 2,4-di-tert-butylphenol. Figure 6 A comparative diagram of the relative EAG responses of adult leafhoppers to different concentrations of 2,4-di-tert-butylphenol. Figure 7 The diagram shows the selective behavior response of adult leafminer's stem fly to different concentrations of 2,4-di-tert-butylphenol; where a is the selective behavior response of male adult leafminer's stem fly to different concentrations of 2,4-di-tert-butylphenol; and b is the selective behavior response of female adult leafminer's stem fly to different concentrations of 2,4-di-tert-butylphenol. Figure 8 A graph showing the difference in selectivity of female and male adult leafhoppers for 2,4-di-tert-butylphenol at the same concentration. Figure 9 A graph showing the differences in selectivity of male and female adult leafhoppers for different concentrations of 2,4-di-tert-butylphenol. Figure 10 The cumulative field trapping effect of different concentrations of 2,4-di-tert-butylphenol on leafhoppers is shown in the figure. Figure 11 The diagram shows the trapping effect of different concentrations of 2,4-di-tert-butylphenol on female and male adult leafhoppers. Detailed Implementation

[0014] The present invention will now be described in detail with reference to the accompanying drawings.

[0015] The larvae of the tested insects were collected from the soil of a quinoa field in Erdaobian Village, Xiwulanbulang Town, Wuchuan County, Inner Mongolia (41°09′33″N, 111°05′06″E).

[0016] The collected larvae were placed in an indoor constant temperature incubator for rearing under the following conditions: temperature 22±0.5℃, relative humidity 70±10%, and complete darkness. After the larvae naturally emerged as male and female adults, they were placed in an insect rearing cage and fed with honey water at a concentration of 10% by weight.

[0017] Morphological observations of the larvae and adults were conducted in the laboratory, and the morphological characteristics were compared with those of *Stereophys serratus* published in existing literature. Molecular biological identification was also performed to confirm that the insect was *Stereophys serratus*. Adult *Stereophys serratus* reared in the laboratory were used for subsequent experiments and were also commercially available.

[0018] All the test compounds used in this invention were purchased from McLean Company, and specific information is shown in Table 1.

[0019] Table 1 Compound Information Table 2,4-Di-tert-butylphenol <![CDATA[C 14 H 22 O]]> 96-76-4 206.33 ≥99 % 2-Nonol <![CDATA[C9H 20 O]]> 628-99-9 144.25 98 % n-Hexane <![CDATA[C6H 14 ]]> 110-54-3 86.18 ≥98%HPLC Example 1: Identification of gonadal active components in extract of *Spodoptera litura* gonads Gonad extraction was performed during the peak mating period of adult leafminer's vegetative state (18:00-21:00). After pre-cooling the dissecting scissors at 4℃ for 10 min, the gonadal tissues of live females and males were cut off under a stereomicroscope. The obtained female and male gland tissues were immersed in n-hexane and extracted at room temperature (25℃) for 1.5 h to obtain female and male adult gonadal extracts, respectively. The obtained female and male adult gonadal extracts were transferred to brown headspace vials, sealed with sealing film, and stored at -20℃ in the dark.

[0020] The above-mentioned female and male adult gonad extracts were analyzed by GC-EAD and GC-MS to identify the active components in the gonads of *Spodoptera litura*.

[0021] GC-EAD detection conditions: Agilent HP-5 capillary column (30 m × 0.32 mm × 0.25 μm) was used, with nitrogen as the carrier gas and a flow rate of 1 mL / min; the injection port temperature was 250℃, splitless injection was used, and the injection volume was 3 μL; the temperature program was as follows: initial temperature 50℃, held for 1 min; temperature increased to 200℃ at a rate of 10℃ / min; then increased to 280℃ at a rate of 15℃ / min and held for 15 min.

[0022] GC-MS detection conditions: Agilent HP-5ms capillary column (30 m × 0.25 mm × 0.25 μm); temperature program: initial temperature 40℃, hold for 5 min; increase to 200℃ at a rate of 10℃ / min; then increase to 280℃ at a rate of 15℃ / min, hold for 20 min; EI ion source temperature 230℃, full scan mode, scan range m / z 28-400.

[0023] GC-EAD assays were performed on the gonadal extracts of female and male adult *Schizothorax oryzae*. Four active compounds that induced significant electrophysiological responses in the antennae of both adults were identified and named Compound I, Compound II, Compound III, and Compound IV. Compounds I, II, III, and IV were detected in the male gonadal extract; Compounds II, III, and IV were detected in the female gonadal extract. In the male gonadal extract, both Compound I and Compound II induced strong antennal potential responses in both male and female adults. The retention time of Compound II in GC-EAD chromatography was 12.85 min (see [link to GC-EAD assay]). Figure 1 a) In the female gonadal extract, compound II also induced a significant antennal potential response in both male and female adults, with a retention time of 12.80 min (see [link to extract]). Figure 1 b); Compounds III and IV were detected in the gonadal extracts of both female and male adults, and both induced antennal potential responses in both adult females and males (see...). Figure 1 c. Figure 1d).

[0024] Because GC-EAD and GC-MS use capillary columns of different specifications, the retention times of the same component differ between the two detection systems. In GC-MS detection, the retention time of compound II in the female gonad extract was 18.639 min (see...). Figure 2 a) Figure 2 b is the standard spectrum of 2,4-di-tert-butylphenol from the NIST library; the mass spectrometry characteristic fragment ions of this component were compared with the standard library, and the measured fragment ion spectrum was in high agreement with the standard spectrum of 2,4-di-tert-butylphenol (see [link]). Figure 2 c), thus confirming that the active ingredient is 2,4-di-tert-butylphenol.

[0025] Using the same analytical method, the GC-EAD activity peaks of the remaining three components that could induce electrophysiological responses in the antennae of both male and female adults were compared with the GC-MS total ion chromatograms to determine the retention times of each active component in the GC-MS system. The measured fragment ion information of each component was compared with the NIST standard mass spectrometry library. It was determined that compound I was 2-nonanol, compound III was heneicosane, and compound IV was n-heptacosane. A comparison of the peak areas in the GC-MS total ion chromatograms of the gonadal extracts of female and male *Schizothorax oryzae* showed that 2-nonanol was a component unique to the male gonadal extract, while the other three components were detectable in both the female and male gonadal extracts; the relative content of 2,4-di-tert-butylphenol in the female gonadal extract was higher than that in the male (see...). Figure 3 ).

[0026] Example 2: Verification of the attraction activity of adult leafhoppers. 1) Antennae potential (EAG) measurement:

[0027] EAG assays were performed on adults 1-2 days after emergence. Using hexane as a solvent, 2,4-di-tert-butylphenol was prepared into five concentration gradients: 0.01 μg / μL, 0.1 μg / μL, 1 μg / μL, 10 μg / μL, and 100 μg / μL. The relative response values ​​of antennal potentials were calculated. 10 μL of the test solution was added to a filter paper strip (0.5 cm × 3 cm), allowed to evaporate at room temperature for 30 s, and then placed into a Pasteur tube. After standing for 60 s, the solution was used for stimulation. The stimulation parameters were: stimulation time 0.2 s, stimulation interval 60 s, stimulation gas flow rate 300 mL / min, and continuous gas flow rate 1000 mL / min. The stimulation sequence was: hexane control → 0.01 μg / μL → 0.1 μg / μL → 1 μg / μL → 10 μg / μL → 100 μg / μL → hexane control.

[0028] The head of an adult leafhopper was connected to a reference electrode, and the tip of its antennae to a recording electrode. After the EAG baseline stabilized, samples were stimulated sequentially and electrophysiological signals were recorded. One-way ANOVA was used to analyze the differences in relative EAG response values ​​of insects of the same sex under different concentrations of test solutions; independent samples were used. t The differences in EAG responses between male and female adults were analyzed under the same concentration of the test solution.

[0029] The formula for calculating the relative value of antennal potential response is as follows: ; In the formula: V is the relative response value of antennal potential; CT is the EAG response value of the sample to be tested; CK1 and CK2 are the EAG response values ​​of the blank control before and after the sample to be tested, respectively.

[0030] EAG test results showed that all tested concentrations of 2,4-di-tert-butylphenol could induce antennal potential responses in both female and male adult leafhoppers (see...). Figure 4 The male insects showed a low EAG response level at a test solution concentration of 0.01 μg / μL; the response increased slightly when the concentration was increased to 0.1 μg / μL, but there was no significant difference compared to the 0.01 μg / μL treatment. P >0.05); when the concentration was increased to 1 μg / μL, the response was still not significantly different from that of the 0.1 μg / μL treatment ( P >0.05); the response peaked at a concentration of 10 μg / μL, significantly higher than that at 0.1 μg / μL ( P <0.05), but there was no significant difference compared to the 1 μg / μL treatment ( P >0.05); when the concentration was increased to a maximum of 100 μg / μL, the EAG response value decreased slightly, but there was no significant difference compared with the other concentration treatments. P >0.05) (see Figure 4 a). Female insects showed a weaker response at low concentrations of 0.01 μg / μL and 0.1 μg / μL, with no significant difference between the two groups. P >0.05); the response increased when the concentration increased to 1 μg / μL, but there was no significant difference compared with the low concentration group ( P >0.05); the response peaked at a concentration of 10 μg / μL, significantly higher than the low concentration group ( P <0.05); when the concentration was increased to a maximum of 100 μg / μL, the EAG response value decreased slightly and remained at a moderate response level, with no significant difference compared to the other concentration treatments. P >0.05) (see Figure 4 b).

[0031] The sample testing order was as follows: n-hexane control (Rec-1) → 0.01 μg / μL (Rec-2) → 0.1 μg / μL (Rec-3) → 1 μg / μL (Rec-4) → 10 μg / μL (Rec-5) → 100 μg / μL (Rec-6) → n-hexane control (Rec-7) (see...) Figure 5 a, Figure 5 b). Both male and female adult leafhoppers of the leafhopper exhibited a typical concentration-dependent EAG response to 2,4-di-tert-butylphenol.

[0032] With increasing 2,4-di-tert-butylphenol concentration, the relative EAG response values ​​of both male and female adults generally showed a trend of first increasing and then decreasing, with both reaching a peak response at a concentration of 10 μg / μL. At all tested concentrations, the EAG response intensity of male insects was significantly higher than that of female insects. P <0.001); the difference in EAG response intensity between female and male adults was most significant at 10 μg / μL (see Figure 6 ).

[0033] 2) Behavioral tendency assessment: Behavioral tactic behavior was assessed using adult leafhoppers on days 1-2 after emergence. Five concentration gradients of 2,4-di-tert-butylphenol (0.01 μg / μL, 0.1 μg / μL, 1 μg / μL, 10 μg / μL, and 100 μg / μL) were prepared using hexane as the solvent. A Y-shaped glass tube olfactometer was used, with arms 18 cm long, an inner diameter of 1.5 cm, and a base tube length of 13.5 cm. The gas path connection sequence was: air pump → silica gel drying tube → activated carbon filter tube → odor bottle → flow meter → Y-shaped tube → atmospheric sampler. The gas flow rate was set to 0.6 L / min. The test environment conditions were: temperature 25±2℃ and relative humidity 65±5%. Filter paper strips containing the test solution and n-hexane were placed in the Y-shaped tube treatment arm and control arm, respectively. Thirty male and thirty female adults were tested in each concentration treatment group, with three replicates. Each insect was observed for 5 minutes, and selection was made when an insect crawled more than one-third of the way across the test arm and remained there for more than one minute. The odor source was changed after every 10 insects were tested, and a new Y-shaped olfactory instrument was used after every 30 insects were tested.

[0034] Differences in behavioral choice between the 2,4-di-tert-butylphenol treatment groups and the hexane solvent control group were statistically analyzed using the chi-square test (χ² test). P <0.05 was the significance criterion; the difference in selectivity for 2,4-di-tert-butylphenol between female and male adult leafhoppers under the same concentration conditions was tested using a 2×2 contingency table chi-square test for independence, and ns indicated that the difference between males and females was not significant. P>0.05); The difference in selectivity of female and male adult leafhoppers for different concentrations of 2,4-di-tert-butylphenol was analyzed by one-way ANOVA combined with Tukey's HSD post hoctest.

[0035] The formula for calculating behavioral selection rate is as follows:

[0036] The results showed that the behavioral selection of both male and female adult *Leymus chinensis* towards 2,4-di-tert-butylphenol (2,4-butylphenol) increased with increasing concentration of the test solution, reaching a peak at 10 μg / μL, and decreasing slightly at 100 μg / μL. However, there were significant differences between the different concentration treatment groups and the solvent control group. At the lowest concentration of 0.01 μg / μL, the selection rate of male insects towards 2,4-di-tert-butylphenol was 54.44%, which was not significantly different from the solvent control group. P >0.05); when the concentration increased to 0.1 μg / μL and 1 μg / μL, the male selection rate increased to 67.78% and 68.89%, respectively, both significantly higher than the solvent control group, exhibiting highly significant tactic behavior. P <0.001); Under the condition of 10 μg / μL, the selection rate of male insects further increased to 75.56%, reaching a peak, showing a highly significant tropism ( P <0.001); when the concentration was further increased to 100 μg / μL, the selectivity decreased to 60.00%, and the difference between the selectivity rate and the solvent control group was not significant. P >0.05 (see) Figure 7 a). At a concentration of 0.01 μg / μL, the selectivity of female insects for 2,4-di-tert-butylphenol was 55.56%, which was not significantly different from the solvent control group. P >0.05); when the concentration increased to 0.1 μg / μL, the female selection rate increased to 64.44%, exhibiting extremely significant directional behavior ( P <0.01); Under conditions of 1 μg / μL and 10 μg / μL, the selection rate of female insects reached 74.45% and 83.33%, respectively, both significantly higher than that of the solvent control group, exhibiting highly significant tactic behavior. P <0.001, with the highest female selection rate at 10 μg / μL; when the concentration was further increased to 100 μg / μL, the selection rate decreased to 64.44%, but was still significantly higher than that of the solvent control group ( P <0.01) (see Figure 7 b).

[0037] Within the test concentration range of 0.01-100 μg / μL, there was no significant sex difference in the behavioral selection rate of adult female and male *Stereophys striatum* towards 2,4-di-tert-butylphenol. P >0.05). At the lowest concentration of 0.01 μg / μL, the selection rates of male and female insects were 54.44% and 55.56%, respectively, with no significant difference between males and females. P >0.05); when the concentration increased to 0.1 μg / μL, the selection rates of both male and female adults improved, reaching 67.78% and 64.44% respectively, but no significant sex difference was still observed. P >0.05); at a concentration of 1 μg / μL, the selection rate of female insects was slightly higher than that of male insects, at 74.45% and 68.89% respectively, but the difference was not statistically significant. P >0.05); when the concentration increased to 10 μg / μL, the selection rates of both male and female adults reached their peak, with females at 83.33% and males at 75.56%, and there was still no significant difference between the sexes ( P >0.05); at the highest concentration of 100 μg / μL, the selectivity of both decreased, with females at 64.44% and males at 60.00%, and there was still no significant difference between males and females ( P >0.05 (see) Figure 8 ).

[0038] When the concentration of the test solution increased from 0.01 μg / μL to 0.1 μg / μL, the selection rate of male insects increased from 54.44% to 67.78%, a significant difference. P <0.05); when the concentration increased from 0.1 μg / μL to 1 μg / μL, the selectivity changed little and the difference was not significant ( P >0.05); when the concentration was further increased to 10 μg / μL, the selectivity reached the highest value of 75.56%, and the difference compared with the 1 μg / μL treatment was still not significant ( P >0.05); when the concentration continued to increase to 100 μg / μL, the selectivity decreased to 60.00%, which was highly significant compared to 10 μg / μL. P <0.01). When the concentration of the test solution increased from 0.01 μg / μL to 0.1 μg / μL, the selection rate of female insects increased from 55.56% to 64.44%, which was statistically significant. P <0.05); when the concentration increased from 0.1 μg / μL to 1 μg / μL, the selectivity increased to 74.45%, which was statistically significant. P <0.05); when the concentration was increased to 10 μg / μL, the selectivity further increased to 83.33% and reached a peak, which was significantly different from the 1 μg / μL treatment. P<0.05); when the concentration was increased to 100 μg / μL, the female selection rate decreased to 64.44%, which was highly significant compared with the 10 μg / μL treatment. P <0.001) (see Figure 9 ).

[0039] Example 3: Field Trapping Experiment of Adult Spotted Leaf Fly The experiment was conducted in a field in Wuchuan County, Inner Mongolia, where quinoa had been continuously cropped for many years, the quinoa planting was dense and evenly distributed, and the population density of leafhoppers was similar. The experiment was divided into several plots, each with an area of ​​600 m² (30 m × 20 m). Quinoa was planted in rows with a row spacing of 60 cm. The plots were spaced 5 m apart, and the traps within each plot were placed at 3 m intervals. Sunflowers were planted around the experimental field as a buffer zone.

[0040] Weigh 0.1 g of 2,4-di-tert-butylphenol and add hexane to a final volume of 1 mL to prepare a 100 µg / µL pheromone stock solution. Then, use hexane for serial dilution to obtain pheromone solutions of 10 μg / µL, 1 μg / µL, 0.1 μg / µL, and 0.01 μg / µL, respectively. Store the prepared pheromone solutions at -20℃ for later use. Using a black trap, add 100 µL of pheromone solution of different concentrations to different lures using a pipette. After addition, place the lures in a cool, dark place and let them stand for 30-60 min until the hexane solvent has completely evaporated and the active components have fully permeated the lures. Then, place the lures in the trap for trapping experiments. The lure treatment methods are shown in Table 2.

[0041] Table 2. Core-driving treatment methods CK0 0 Coreless bare blank board 0 B1 0 n-Hexane (CK) 100 ul B2 0.01 ug / ul 2,4-Di-tert-butylphenol 100 ul B3 0.1 ug / ul 2,4-Di-tert-butylphenol 100 ul B4 1 ug / ul 2,4-Di-tert-butylphenol 100 ul B5 10 ug / ul 2,4-Di-tert-butylphenol 100 ul B6 100 μg / μL 2,4-Di-tert-butylphenol 100 ul One-way ANOVA combined with Tukey HSD post-hoc multiple comparison method was used to statistically analyze the field trapping data.

[0042] The experimental results showed that the field trapping effects of lures prepared with different concentrations of 2,4-di-tert-butylphenol pheromone solution on adult leafminer flies varied significantly. The 10 μg / μL pheromone solution treatment group had the highest cumulative insect attraction over 6 days, at 55.00 insects / trapper, significantly higher than the other treatment groups; the 1 μg / μL pheromone solution treatment group was second, with a cumulative insect attraction of 37.33 insects / trapper over 6 days; the 0.1 μg / μL pheromone solution treatment group had a cumulative insect attraction of 27.67 insects / trapper over 6 days; the 100 μg / μL pheromone solution treatment group had a cumulative insect attraction of 20.33 insects / trapper over 6 days; and the 0.01 μg / μL pheromone solution treatment group had a cumulative insect attraction of 15.67 insects / trapper over 6 days. The cumulative insect attraction of the solvent control B1 and the bare blank control CK0 (without lure) was only 2.33 insects / trapper and 1.33 insects / trapper, respectively, with no significant difference between them (see [link to relevant documentation]). Figure 10 ).

[0043] Both male and female *Leymus chinensis* exhibited a clear concentration-response pattern to lures prepared with different concentrations of 2,4-di-tert-butylphenol pheromone solution. For males, the 10 μg / μL pheromone solution treatment group had the highest cumulative number of males attracted over 6 days, at 30.67 males / trap; the 1 μg / μL pheromone solution treatment group had a cumulative number of 22.00 males / trap; the 0.1 μg / μL pheromone solution treatment group had a cumulative number of 16.33 males / trap; the 100 μg / μL and 0.01 μg / μL pheromone solution treatment groups had a cumulative number of 12.00 and 10.33 males / trap, respectively, with no significant difference between the two groups; the solvent control B1 and the lure-free blank control CK0 attracted only 1.67 and 0.67 males / trap, respectively, significantly lower than the 2,4-di-tert-butylphenol treatments. Regarding female insects, the highest cumulative insect count over 6 days was observed in the 10 μg / μL pheromone solution treatment group (24.33 insects / trap); the 1 μg / μL pheromone solution treatment group had a cumulative insect count of 15.33 insects / trap; the 0.1 μg / μL pheromone solution treatment group had a cumulative insect count of 11.33 insects / trap; the 100 μg / μL pheromone solution treatment group had a cumulative insect count of 8.33 insects / trap; and the 0.01 μg / μL pheromone solution treatment group had a cumulative insect count of 5.33 insects / trap. The cumulative insect count in both the solvent control and the blank control (CK0) was 0.67 insects / trap, with no significant difference between the two (see [link to relevant documentation]). Figure 11 ).

[0044] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, and is not intended to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions made by those skilled in the art to the technical solution of the present invention do not depart from the essence and scope of the technical solution of the present invention.

Claims

The application of 1,2,4-di-tert-butylphenol as an attractant for adult leafhoppers is characterized by: The concentration of 2,4-di-tert-butylphenol in the attractant is 0.01 μg / μL-100 μg / μL.

2. The application according to claim 1, characterized in that: The concentration of 2,4-di-tert-butylphenol in the attractant is 10 μg / μL.

3. An attractant for adult leafhoppers, characterized in that: The active ingredient in the attractant for adult leafhoppers is 2,4-di-tert-butylphenol, and the concentration of 2,4-di-tert-butylphenol in the attractant is 0.01 μg / μL-100 μg / μL.

4. The attractant for adult leafhoppers according to claim 3, characterized in that: The concentration of 2,4-di-tert-butylphenol is 10 μg / μL.

5. The attractant for adult leafhoppers according to claim 4, characterized in that: The attractant also contains the organic solvent n-hexane.

6. A decoy for leafhopper flies, characterized in that: The leafhopper attractant core is loaded with the leafhopper adult attractant as described in claim 3, 4 or 5.

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