A plant growth regulator composition for promoting growth and / or improving quality of cinnamomum cassia and use thereof

Abstract

The application discloses a plant growth regulator composition for promoting growth of Cinnamomum cassia and / or improving quality of Cinnamomum cassia and application thereof. The application takes Cinnamomum cassia as a research object, finds that three plant growth regulators, namely naphthalene acetic acid (NAA), gibberellin (GA3) and 6-benzylaminopurine (6-BA), in combination can promote stem diameter growth of Cinnamomum cassia seedlings, increase fresh weight and phloem proportion, and can increase contents of cinnamyl aldehyde, cinnamic acid and cinnamyl alcohol in Cinnamomum cassia bark and reduce content of coumarin in Cinnamomum cassia bark. The application provides an efficient and safe technical scheme for Cinnamomum cassia high-quality seedling cultivation and directional promotion of Cinnamomum cassia seedling quality, and has a good application prospect.

Description

Technical Field

[0001] This invention belongs to the field of plant growth regulator technology, and specifically relates to a plant growth regulator composition that promotes cinnamon growth and / or improves cinnamon quality and its application. Background Technology

[0002] Cinnamon (Cinnamomum cassia (L.) D. Don), a traditional Chinese medicine from Lingnan, is primarily used for its medicinal properties, particularly the phloem of the bark. Its main active ingredients are cinnamaldehyde, cinnamic acid, and coumarin compounds. Cinnamaldehyde and cinnamic acid are used in fragrances, food additives, and the pharmaceutical industry. Coumarin compounds, such as coumarin, are used as flavoring agents in cigarettes, plastic products, and rubber products; however, they pose a risk of liver toxicity, and long-term excessive intake (e.g., more than 0.1 mg / kg body weight daily) may lead to abnormal liver function.

[0003] Currently, the cultivation and propagation of cinnamon seedlings are plagued by technical problems such as slow seedling growth, low biomass of medicinal parts, and insufficient accumulation of key medicinal components. Exogenous plant growth regulators are widely used to promote plant growth and increase the accumulation of secondary metabolites. However, current treatments often involve single hormones, resulting in a significant trade-off between growth and metabolism—either excessive vegetative growth without any added benefits or a one-sided increase in metabolism at the expense of healthy growth. Therefore, what is currently lacking is a combination of plant growth regulators that can simultaneously promote both the growth of cinnamon seedlings and the accumulation of active ingredients, along with corresponding treatment methods. Summary of the Invention

[0004] The primary objective of this invention is to overcome the shortcomings and deficiencies of the prior art and to provide the application of naphthaleneacetic acid (NAA), gibberellin (GA3), and 6-benzylaminopurine (6-BA) in combination to promote cinnamon growth and / or improve cinnamon quality.

[0005] Another objective of this invention is to provide a plant growth regulator composition that improves the growth and quality of cinnamon, aiming to solve the problems of slow growth, poor phloem development, and unsatisfactory medicinal components in cinnamon seedling cultivation.

[0006] Another object of the present invention is to provide the application of the plant growth regulator composition for improving the growth and quality of cinnamon.

[0007] The objective of this invention is achieved through the following technical solution:

[0008] Application of naphthaleneacetic acid (NAA), gibberellin (GA3) and 6-benzylaminopurine (6-BA) in promoting cinnamon growth and / or improving cinnamon quality.

[0009] The promotion of cinnamon growth includes promoting the growth of cinnamon stem thickness (increasing cinnamon stem thickness), increasing the fresh weight of cinnamon and / or the proportion of phloem.

[0010] Furthermore, the mass ratio of naphthaleneacetic acid (NAA), gibberellin (GA3), and 6-benzylaminopurine (6-BA) is (25-100):(5-20):(10-50); preferably 50:10:50.

[0011] The improvement of cinnamon quality includes at least one of the following aspects:

[0012] (a) Increase the content of cinnamaldehyde in cinnamon bark;

[0013] (b) Increase the content of cinnamic acid in cinnamon bark;

[0014] (c) Increase the content of cinnamon bark in cinnamon bark;

[0015] (d) Reduce the content of coumarin in cinnamon bark.

[0016] Furthermore, to increase the content of cinnamaldehyde in cinnamon bark, the mass ratio of naphthaleneacetic acid, gibberellin and 6-benzylaminopurine is (25-50):(10-20):(25-50) or 100:20:25; preferably 25:10:25, 25:20:50, 50:10:50 or 100:20:25.

[0017] Furthermore, to increase the cinnamic acid content in cinnamon bark, the mass ratio of naphthaleneacetic acid, gibberellin, and 6-benzylaminopurine is (50-100):(5-20):(25-50); preferably 50:5:25, 50:10:50, or 100:20:25.

[0018] Furthermore, to increase the content of cinnamyl alcohol in cinnamon bark, the mass ratio of naphthaleneacetic acid, gibberellin, and 6-benzylaminopurine is 25:(10-20):(25-50); preferably 25:10:25 or 25:20:50.

[0019] Furthermore, to reduce the coumarin content in cinnamon bark, the mass ratio of naphthaleneacetic acid, gibberellin, and 6-benzylaminopurine is (25-100):(10-20):(25-50).

[0020] Furthermore, the concentration of naphthaleneacetic acid (NAA) used is 25–100 mg / L; preferably 40–60 mg / L; more preferably 50 mg / L.

[0021] Furthermore, the solvent used to dissolve naphthaleneacetic acid (NAA) is an ethanol solution; preferably an ethanol solution of 60 to 100% by volume; more preferably an ethanol solution of 80% by volume.

[0022] Furthermore, the concentration of the gibberellin (GA3) used is 5–20 mg / L; preferably 5–10 mg / L; more preferably 10 mg / L.

[0023] Furthermore, the solvent used to dissolve gibberellin (GA3) is an ethanol solution; preferably an ethanol solution of 50-80% by volume; more preferably an ethanol solution of 60% by volume.

[0024] Furthermore, the concentration of 6-benzylaminopurine (6-BA) used is 10-50 mg / L; preferably 25-50 mg / L; more preferably 50 mg / L.

[0025] Furthermore, the solvent used to dissolve 6-benzylaminopurine (6-BA) is a sodium hydroxide solution; preferably a sodium hydroxide solution of 0.1 to 2 mol / L; more preferably a sodium hydroxide solution of 1 mol / L.

[0026] Furthermore, the cinnamon is preferably a cinnamon seedling; more preferably a one-year-old cinnamon seedling.

[0027] A plant growth regulator composition that promotes cinnamon growth and / or improves cinnamon quality, comprising any one of the following:

[0028] (1) A plant growth regulator composition for promoting the growth of cinnamon, the composition comprising naphthaleneacetic acid, gibberellin and 6-benzylaminopurine in a mass ratio of (25-100):(5-20):(10-50); preferably 50:10:50;

[0029] (2) A plant growth regulator composition for increasing the cinnamaldehyde content in cinnamon bark, the composition comprising naphthaleneacetic acid, gibberellin and 6-benzylaminopurine in a mass ratio of (25-50):(10-20):(25-50) or 100:20:25; preferably 25:10:25, 25:20:50, 50:10:50 or 100:20:25;

[0030] (3) A plant growth regulator composition for increasing the cinnamic acid content in cinnamon bark, the composition comprising naphthaleneacetic acid, gibberellin and 6-benzylaminopurine in a mass ratio of (50-100):(5-20):(25-50); preferably 50:5:25, 50:10:50 or 100:20:25;

[0031] (4) A plant growth regulator composition for increasing the content of cinnamyl alcohol in cinnamon bark, the composition comprising naphthaleneacetic acid, gibberellin and 6-benzylaminopurine in a mass ratio of 25:(10-20):(25-50); preferably 25:10:25 or 25:20:50.

[0032] (5) A plant growth regulator composition for reducing the coumarin content in cinnamon bark, the composition comprising naphthaleneacetic acid, gibberellin and 6-benzylaminopurine in a mass ratio of (25-100):(10-20):(25-50).

[0033] Furthermore, the promotion of cinnamon growth mentioned in (1) includes promoting the growth of cinnamon stem thickness (increasing cinnamon stem thickness), increasing the fresh weight of cinnamon and / or the proportion of phloem.

[0034] Furthermore, the plant growth regulator composition may also contain other solvents or adjuvants.

[0035] Furthermore, the solvents include ethanol, sodium hydroxide, water, etc.

[0036] Furthermore, the additives include spreading agents such as Tween-20 and Tween-80.

[0037] The application of the plant growth regulator composition in promoting cinnamon growth (cinnamon seedling cultivation) and / or improving cinnamon quality.

[0038] A method for promoting cinnamon growth and / or improving cinnamon quality includes the following steps: preparing the above-mentioned plant growth regulator composition into a mixed solution, and foliar spraying it during the cinnamon growing season to promote cinnamon growth and / or increase the content of cinnamaldehyde, cinnamic acid, and cinnamyl alcohol in cinnamon bark, and / or decrease the content of coumarin in cinnamon bark.

[0039] Furthermore, the cinnamon is preferably a cinnamon seedling; preferably a one-year-old cinnamon seedling, which is treated after transplanting and has been in the recovery period for one month.

[0040] Furthermore, the foliar spraying method is as follows: spray once every 7 days, continuously for 2 to 7 months (preferably 6 months), and the preferred spraying time is after 4 pm or on a cloudy day to avoid high temperature and strong light.

[0041] The method for promoting cinnamon growth and / or improving cinnamon quality further includes a step of sampling and determining the content of cinnamaldehyde, cinnamic acid, and coumarin in cinnamon bark. Specifically, after foliar spraying of an aqueous solution of a plant growth regulator composition for 2 to 4 months (preferably 3 months), samples are taken (immediately placed in dry ice and then stored at -80°C), and the content of cinnamaldehyde, cinnamic acid, coumarin, and cinnamyl alcohol in cinnamon bark is then determined.

[0042] The present invention has the following advantages and effects compared with the prior art:

[0043] 1. This invention addresses the common technical problems in cinnamon seedling cultivation, such as slow growth, low biomass of medicinal parts, and insufficient accumulation of main medicinal effects. Through orthogonal experimental design, using one-year-old cinnamon seedlings as the research object, this invention systematically investigates the effects of three plant growth regulators—naphthaleneacetic acid (NAA), gibberellin (GA3), and 6-benzylaminopurine (6-BA)—at different concentration ratios on seedling growth indicators (stem diameter, seedling height), phloem biomass, anatomical structure (phloem thickness / diameter), and the main metabolic components (cinnamaldehyde, cinnamic acid, and coumarin) of different parts (roots, stem bark, and leaves). The results showed that the composition of the present invention could promote the growth of stem diameter of cinnamon seedlings (increased by about 236.7%), increase fresh weight (increased to 48.3%) and phloem ratio (increased to 31.0%), and enhance phloem development; at the same time, it could improve the medicinal components in cinnamon bark: increase the content of cinnamic acid in the bark (increased by about 33.5%), significantly reduce the content of non-target component coumarin (reduced by about 86.4%), and slightly increase the content of cinnamaldehyde (increased by about 2.3%).

[0044] 2. The plant growth regulator composition (NAA, GA3, 6-BA) used in this invention are all commonly used varieties, with concentrations within the safe range and no visible phytotoxicity. The combination of the three plant growth regulators can produce a synergistic effect, providing an efficient and safe technical solution for the cultivation of high-quality cinnamon seedlings and the targeted improvement of cinnamon seedling quality, and has good application prospects. Attached Figure Description

[0045] Figure 1 This is a bar chart comparing the stem diameter growth and the percentage of fresh bark weight of cinnamon seedlings after 3 months under different treatments (T5 treatment was significantly higher than the control).

[0046] Figure 2 This is a statistical chart showing the content of major compounds in cinnamon stem bark after 3 months of different treatments.

[0047] Figure 3 This is a graph showing the ratio of cinnamon stem bark to diameter after 3 months of different treatments (in the graph, D1 and D2 are the stem diameter width; d1 and d2 are the stem xylem width).

[0048] Figure 4 The weighting of evaluation indicators for the quality of cinnamon stem bark after 3 months of different treatments.

[0049] Figure 5This is a radar chart showing the advantages of each indicator in group T5 after 3 months of different treatments (in the chart, Thick_stem: stem diameter growth; Po_fresh_weight: fresh weight percentage; Po_phloem: phloem percentage; RGQ_sink: cinnamaldehyde in the bark; XDS_sink: coumarin in the bark; RGS_sink: cinnamic acid in the bark; RGC_sink: cinnamyl alcohol in the bark).

[0050] Figure 6 This is a statistical chart of growth indicators (stem diameter and seedling height) of cinnamon seedlings after being sprayed with 6-BA alone.

[0051] Figure 7 This is a graph showing the ratio of cinnamon stem bark to diameter after cinnamon seedlings were sprayed with 6-BA alone.

[0052] Figure 8 This is a statistical chart showing the content of the main metabolites (cinnamaldehyde, cinnamic acid, and cinnamyl alcohol) after cinnamon seedlings were sprayed with 6-BA alone. Detailed Implementation

[0053] The present invention will be further described in detail below with reference to embodiments, but the implementation of the present invention is not limited thereto. Unless otherwise specified, the reagents, methods, and equipment used in the present invention are conventional reagents, methods, and equipment in this technical field. Test methods in the following embodiments that do not specify specific experimental conditions are generally performed according to conventional experimental conditions or experimental conditions recommended by the manufacturer. Unless otherwise specified, the reagents and raw materials used in the present invention are commercially available.

[0054] The plant growth regulators involved in the embodiments of the present invention, such as naphthaleneacetic acid (NAA), gibberellin (GA3) and 6-benzylaminopurine (6-BA), can be obtained from conventional commercial sources.

[0055] The cinnamon involved in this embodiment of the invention is Chinese cinnamon, Cinnamomum cassia (L.) D. Don, which is widely cultivated in the Guangdong and Guangxi regions.

[0056] Example 1: Preparation and Field Application of the Composition

[0057] (1) Seedling preparation: Select one-year-old cinnamon seedlings with uniform growth and transplant them into substrate bags [the volume ratio of substrate is seedling substrate soil: yellow soil = 2:1; the seedling substrate soil is obtained by mixing imported peat, imported coconut coir, perlite and carbonized rice husk (mass ratio of 5:4:2:3) and a small amount of lignocellulose and vermiculite], and manage them routinely for 1 month.

[0058] (2) Preparation of the conditioning agent: Weigh 50 mg of NAA, 20 mg of GA3, and 25 mg of 6-BA respectively. Dissolve NAA and GA3 in small amounts of 80% and 60% ethanol solutions respectively, and dissolve 6-BA in 1 mol / L sodium hydroxide solution to prepare a stock solution of 50 mg / ml NAA, 20 mg / ml GA3, and 25 mg / ml 6-BA. Store at -4℃. When using, mix the three stock solutions with sterile water and dilute to the expected concentration (NAA 50 mg / L, GA3 10 mg / L, 6-BA 50 mg / L). Add Tween-20 at a final concentration of 0.05% (v / v) as a spreading agent and stir well.

[0059] (3) Spraying treatment: Spray the upper and lower surfaces of cinnamon leaves every 7 days until dripping is achieved, and continue spraying for 3 months. The control group was sprayed with an equal amount of sterile water (CK1). The solvent control group was sprayed with an equal concentration of co-solvent ethanol and sodium hydroxide (CK2).

[0060] (4) Effect determination: ① Before and after treatment, the stem diameter of seedlings 3cm above the ground was measured using vernier calipers; ② A 6cm stem segment above the root was taken, the bark and xylem were separated and weighed separately, and the fresh weight of the phloem was recorded; ③ The samples were fixed in 4% PFA fixative for more than 24 hours, sectioned using a sliding microtome, and observed under an optical microscope. The diameter of the xylem and the diameter of the stem were recorded, and the proportion of phloem was calculated; ④ Samples of different parts of cinnamon seedlings (roots, stem bark, mature leaves) were collected, and the contents of cinnamaldehyde, cinnamic acid, coumarin, etc., were determined using LC-MS. The experiment was repeated three times. The LC-MS determination method is as follows:

[0061] (a) Sample pretreatment: Take 100 mg of cinnamon material and grind it with liquid nitrogen; add 600 μL of extraction solution (methanol:isopropanol:glacial acetic acid = 20:79:1, v / v / v; pre-cool before use), vortex; sonicate at 4 °C for 30 min, centrifuge at 4 °C and 10000 r / min for 15 min, and take the supernatant; add 400 μL of extraction solution to the precipitate and vortex; sonicate at 4 °C for 30 min, centrifuge and take the supernatant; filter through a 0.22 filter membrane (green) twice.

[0062] (b) LC-MS parameters and conditions for metabolite determination:

[0063] LC analysis conditions: Column: XSelect HSS T3 2.5 µm (2.1 × 50 mm) (Waters Corporation, USA); Injection volume: 2.0 μL; Flow rate: 0.3 mL / min; Column temperature: 35 ℃; Mobile phase: Phase A 0.1% (v / v) formic acid aqueous solution; Phase B acetonitrile, 90%A+10%B (0~1 min), 70%A+30%B (1~3 min), 2%A+98%B (3~4.5 min), 2%A+98%B (4.5~7.5 min), 90%A+10%B (7.5~8.5 min), 90%A+10%B (8.5~12 min).

[0064] Mass spectrometry conditions: Ion source: electrospray ionization source; Scanning mode: positive and negative ion scanning; Detection mode: multiple reaction monitoring; Capillary voltage: 2 kV; Desolvation gas temperature: 300 ℃; Desolvation gas flow rate: 700 L / H; Cone gas flow rate: 50 L / H; Parameters such as spray voltage, ion source temperature, desolvation temperature, and nitrogen flow rate should be optimized to achieve the optimal sensitivity; Qualitative ion pairs, quantitative ion pairs, and collision energies are shown in Table 1.

[0065] Table 1 Qualitative ion pairs, quantitative ion pairs, and collision energies

[0066]

[0067] The results are as follows Figure 1-3 As shown, the results indicated that the stem diameter increase in the treatment group reached 0.633 mm, the fresh weight percentage was 0.483%, the phloem percentage was 0.310%, and the cinnamaldehyde content in the bark was 1887.5 μg*g. -1 The coumarin content is 38.2 μg*g. -1 It was significantly better than the control group.

[0068] Example 2: Orthogonal Experiment and Comprehensive Scoring

[0069] Nine combinations of NAA (25, 50, 100 mg / L), GA3 (5, 10, 20 mg / L), and 6-BA (10, 25, 50 mg / L) were set up using an L9(3³) orthogonal array, with blank and solvent controls (CK1, CK2) as controls (Table 2). Each treatment was repeated 15 times, and the remaining steps and detection methods were the same as in Example 1.

[0070] Table 2 Specific formulations for each treatment group

[0071]

[0072] Three plants were randomly selected after three months of treatment for testing. A total of three growth indicators (stem diameter, fresh weight percentage, and phloem percentage) and 12 metabolic indicators were obtained: cinnamic acid in roots (RGS_root), cinnamic acid in bark (RGS_sink), cinnamic acid in leaves (RGS_leaf), cinnamaldehyde in roots (RGQ_root), cinnamaldehyde in bark (RGQ_sink), cinnamaldehyde in leaves (RGQ_leaf), cinnamyl alcohol in roots (RGC_root), cinnamyl alcohol in bark (RGC_sink), cinnamyl alcohol in leaves (RGC_leaf), coumarin in roots (XDS_root), coumarin in bark (XDS_sink), and coumarin in leaves (XDS_leaf).

[0073] Range analysis: Through range analysis of orthogonal experiments, the optimal single hormone treatment level corresponding to each indicator was selected, and the groups that meet the expectations (thick phloem, high cinnamaldehyde and low coumarin) were screened out.

[0074] Growth and metabolism comprehensive score: According to the research purpose, the weight of each evaluation index was determined by the combination weighting method for the three growth indicators and twelve metabolic indicators of cinnamon plants. The subjective judgment of the analytic hierarchy process (AHP) and the objective information of the entropy weight method were combined to take into account both experience and the inherent laws of the data.

[0075] ① Analytic Hierarchy Process (AHP):

[0076] The Analytic Hierarchy Process (AHP) transforms qualitative judgments into quantitative weights by constructing a hierarchical structure and pairwise comparison judgment matrices. A criterion layer was set up: B1_growth, B2_phloem, B3_root, and B4_leaf, reflecting the macroscopic trait framework of the plant. An indicator layer consisted of 15 specific measurement indicators, each belonging to one of the criteria. A 1-9 scale was used to construct the judgment matrix, and logical consistency was tested by calculating the maximum eigenvalue and the consistency ratio (CR). When CR < 0.1, the judgment matrix was considered to have passed the consistency test. The consistency test results for each judgment matrix are shown in Table 3. In this study, the CR values ​​of all judgment matrices ranged from 0.004 to 0.012, all far below the threshold of 0.1, indicating logical consistency and reliable weight results. SPSSPRO online software was used for analysis. After hierarchical single ranking and consistency testing, the weights of each layer were calculated, and a hierarchical overall ranking was performed to obtain the global subjective weight W for each indicator. j AHP (Table 4).

[0077] Table 3 Consistency test results of each judgment matrix in AHP

[0078]

[0079] Table 4. Weight Calculation Table for Each Indicator by Analytic Hierarchy Process (AHP)

[0080]

[0081] ② Entropy weight method

[0082] The entropy weight method determines weights based on the degree of variation of the indicators—the smaller the information entropy, the greater the dispersion of the indicator, and the higher the weight. Positive weights are assigned to the proportion of phloem and the content of cinnamaldehyde in the bark, while negative weights are assigned to the content of coumarin in each part. The calculation steps are briefly described below:

[0083] (1) Range normalization of the original data:

[0084] Positive indicators: ;

[0085] Negative indicators: ;

[0086] (2) Calculate the feature weight of the i-th sample under the j-th indicator: ;

[0087] (3) Calculate the information entropy value: ;

[0088] (4) Calculate the information utility value After normalization, the entropy weight method weights are obtained: ;

[0089] Note: The value of a certain indicator. The minimum value of a certain indicator. This represents the maximum value of a certain indicator. `i` represents the column for different indicator processing, and `j` represents the row of indicator values ​​for different processing.

[0090] The results of the entropy weight method weight calculation for each indicator are shown in Table 5.

[0091] Table 5. Calculation results of entropy weight method for each indicator

[0092]

[0093] ③Combined weighting

[0094] To balance expert experience with objective data patterns, a linear weighted combination method is used to synthesize subjective and objective weights:

[0095]

[0096] In this study, all AHP judgment matrices passed the consistency test, indicating that expert experience has high reliability. Therefore, α = 0.7 (70% subjective weight and 30% objective weight). The combined weight results are shown in Tables 6 and 7.

[0097] Table 6. Weighting Table for Comprehensive Values ​​of Each Indicator

[0098]

[0099] Table 7 Scoring Table for Combination Weighting Method

[0100]

[0101] The results are as follows Figure 1-5 As shown: The results indicate that the composition of the present invention (T5: NAA 50 mg / L, GA3 10 mg / L, 6-BA 50 mg / L) significantly promoted stem diameter growth: after 3 months of treatment, the stem diameter growth of cinnamon seedlings reached 0.633 mm, which was approximately 236.7% higher than that of the control group (0.188 mm) (p < 0.001). This composition also increased fresh weight and phloem percentage: the fresh weight percentage increased to 48.3% (control group 42.7%), and the phloem percentage increased to 31.0% (control group 28.7%), indicating enhanced phloem development. Furthermore, this composition can increase the stem diameter, fresh weight, phloem ratio, and cinnamic acid content in cinnamon seedlings, while decreasing the coumarin content: the cinnamaldehyde (RGQ_sink) content in the bark reached 1887.5 μg / g (compared to 1845.2 μg / g in the control group), maintaining a high level; the cinnamic acid (RGS_sink) content increased to 868.4 μg / g (compared to 650.7 μg / g in the control group), an increase of 33.5%; the coumarin (XDS_sink) content significantly decreased to 38.2 μg / g (compared to 281.4 μg / g in the control group), a decrease of 86.4%; and the cinnamyl alcohol (RGC_sink) content significantly decreased to 31.0 μg / g (compared to 87.6 μg / g in the control group), a decrease of 56.4%.

[0102] The above results indicate that the composition of this invention (T5: NAA 50 mg / L, GA3 10 mg / L, 6-BA 50 mg / L) achieved a comprehensive score of 0.638, ranking first among all 11 treatments, and placing it in the top three along with T2 (0.6090) and T9 (0.6005). Its common characteristics include a large phloem thickness, high cinnamaldehyde content, and low coumarin content. The control groups CK1 (0.4538) and CK2 (0.4517) had moderate scores, reflecting a significant impact of the experimental treatments on quality. T1 (0.3805), T6 (0.3894), and T4 (0.4403) scored lower than the control, suggesting that these treatments may have adverse effects on cinnamon quality, and it is recommended that they be excluded or optimized in future studies.

[0103] Example 3: Stability Verification

[0104] Two batches of cinnamon seedlings were treated repeatedly with the composition of the present invention (T5: NAA 50 mg / L, GA3 10 mg / L, 6-BA 50 mg / L) under the same conditions. The results showed good repeatability, and the stem diameter growth and quality indicators reached the level of Example 1, proving that the technical solution is stable and reliable.

[0105] Comparative Example 1: Single Hormone Treatment

[0106] Following the method described in Example 1, single hormones were applied for spraying: 6-BA (20 mg / L, 30 mg / L, 50 mg / L). First, 6-BA was dissolved in 1 mol / L sodium hydroxide solution to prepare a 25 mg / ml 6-BA stock solution. Before use, it was diluted with sterile water to the required concentration. An equal volume of sterile water was used as a control (CK). One-year-old cinnamon seedlings were selected as the experimental subjects. Spraying was performed continuously for one month, with all other steps remaining the same. Cinnamon growth indicators and the content of major metabolites (cinnamaldehyde, cinnamyl alcohol, and cinnamic acid) in the cinnamon bark (sink) and leaves were measured. Each treatment was repeated in five replicates.

[0107] The results showed that after one month of treatment with 6-BA hormone at concentrations of 20 mg / L and 30 mg / L, the stem diameter increases were 0.708 mm and 0.89 mm, respectively. Figure 6 The quality indicators of single-hormone treatments were significantly lower than those of the composition of this invention. The 30 mg / L concentration showed superior effects on stem diameter and stem length growth. Treatment with 20 mg / L 6-BA increased the phloem proportion, but the effect was not significant, and the higher the concentration, the lower the phloem proportion. Figure 7 The content of cinnamaldehyde increased slightly under treatment at 30 mg / L, while the accumulation of cinnamyl alcohol was more significant under treatment at 50 mg / L. Cinnamic acid remained essentially unchanged under treatments at 20 mg / L, 30 mg / L, and 50 mg / L. Figure 8 This indicates that single hormone treatment cannot increase the content of cinnamaldehyde and cinnamic acid in cinnamon bark, while simultaneously decreasing the content of coumarin in cinnamon bark.

[0108] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments. Any changes, modifications, substitutions, combinations, or simplifications made without departing from the spirit and principle of the present invention shall be considered equivalent substitutions and shall be included within the protection scope of the present invention.

Claims

1. The application of naphthaleneacetic acid, gibberellin, and 6-benzylaminopurine in combination for promoting cinnamon growth and / or improving cinnamon quality, characterized in that, The improvement of cinnamon quality includes at least one of the following aspects: (a) Increase the content of cinnamaldehyde in cinnamon bark; (b) Increase the content of cinnamic acid in cinnamon bark; (c) Increase the content of cinnamon bark in cinnamon bark; (d) Reduce the content of coumarin in cinnamon bark.

2. The application according to claim 1, characterized in that: The promotion of cinnamon growth includes promoting the thickening of cinnamon stems, increasing the fresh weight of cinnamon and / or the proportion of phloem; The cinnamon mentioned is a cinnamon seedling.

3. The application according to claim 1, characterized in that: For promoting cinnamon growth, the mass ratio of naphthaleneacetic acid, gibberellin and 6-benzylaminopurine is 25-100:5-20:10-50; To increase the content of cinnamaldehyde in cinnamon bark, the mass ratio of naphthaleneacetic acid, gibberellin and 6-benzylaminopurine is 25-50:10-20:25-50 or 100:20:25; To increase the cinnamic acid content in cinnamon bark, the mass ratio of naphthaleneacetic acid, gibberellin, and 6-benzylaminopurine is 50-100:5-20:25-50; To increase the content of cinnamyl alcohol in cinnamon bark, the mass ratio of naphthaleneacetic acid, gibberellin, and 6-benzylaminopurine is 25:10-20:25-50; To reduce the coumarin content in cinnamon bark, the mass ratio of naphthaleneacetic acid, gibberellin, and 6-benzylaminopurine is 25–100:10–20:25–50.

4. The application according to claim 3, characterized in that: To promote cinnamon growth, the mass ratio of naphthaleneacetic acid, gibberellin, and 6-benzylaminopurine is 50:10:

50. To increase the content of cinnamaldehyde in cinnamon bark, the mass ratio of naphthaleneacetic acid, gibberellin and 6-benzylaminopurine is 25:10:25, 25:20:50, 50:10:50 or 100:20:

25. To increase the content of cinnamic acid in cinnamon bark, the mass ratio of naphthaleneacetic acid, gibberellin and 6-benzylaminopurine is 50:5:25, 50:10:50 or 100:20:

25. To increase the content of cinnamyl alcohol in cinnamon bark, the mass ratio of naphthaleneacetic acid, gibberellin, and 6-benzylaminopurine is 25:10:25 or 25:20:

50.

5. The application according to claim 1, characterized in that: The concentration of naphthaleneacetic acid used is 25–100 mg / L; more specifically, 50 mg / L. The concentration of the gibberellin used is 5–20 mg / L; more specifically, 10 mg / L. The concentration of 6-benzylaminopurine used is 10–50 mg / L; more specifically, 50 mg / L.

6. The application according to claim 1, characterized in that: The solvent used to dissolve naphthaleneacetic acid is an ethanol solution; The solvent used to dissolve gibberellin is an ethanol solution; Sodium hydroxide solution was used as the solvent to dissolve 6-benzylaminopurine.

7. The application according to claim 6, characterized in that: The solvent used to dissolve naphthaleneacetic acid is an ethanol solution with a volume percentage of 60-100%. The solvent used to dissolve gibberellin is an ethanol solution with a volume percentage of 50-80%. The solvent used to dissolve 6-benzylaminopurine is a 0.1–2 mol / L sodium hydroxide solution.

8. A plant growth regulator composition that promotes cinnamon growth and / or improves cinnamon quality, characterized in that, It can be any of the following: (1) A plant growth regulator composition for promoting the growth of cinnamon, the composition comprising naphthaleneacetic acid, gibberellin and 6-benzylaminopurine in a mass ratio of 25-100:5-20:10-50; (2) A plant growth regulator composition for increasing the cinnamaldehyde content in cinnamon bark, the composition comprising naphthaleneacetic acid, gibberellin and 6-benzylaminopurine in a mass ratio of 25-50:10-20:25-50 or 100:20:25; (3) A plant growth regulator composition for increasing the cinnamic acid content in cinnamon bark, the composition comprising naphthaleneacetic acid, gibberellin and 6-benzylaminopurine in a mass ratio of 50-100:5-20:25-50; (4) A plant growth regulator composition for increasing the content of cinnamyl alcohol in cinnamon bark, the composition comprising naphthaleneacetic acid, gibberellin and 6-benzylaminopurine in a mass ratio of 25:10 to 20:25 to 50; (5) A plant growth regulator composition for reducing the coumarin content in cinnamon bark, the composition comprising naphthaleneacetic acid, gibberellin and 6-benzylaminopurine in a mass ratio of 25-100:10-20:25-50.

9. A method for promoting cinnamon growth and / or improving cinnamon quality, characterized in that, The method includes the following steps: preparing a mixed solution of the plant growth regulator composition according to claim 8, and foliar spraying it during the cinnamon growing season to promote cinnamon growth and / or increase the content of cinnamaldehyde, cinnamic acid, and cinnamyl alcohol in cinnamon bark, and / or decrease the content of coumarin in cinnamon bark.

10. The method according to claim 9, characterized in that: The foliar spraying method is as follows: spray once every 7 days for 2 to 7 months. Spraying time is after 4 pm or on cloudy days to avoid high temperature and strong light.

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