A source control method for juniper pollen allergy

Abstract

The application discloses a source control method for Sabina pingii pollen allergy, and belongs to the technical field of source control of urban landscaping maintenance and allergic diseases. The method adopts a source control concept, and special compound agents are sprayed on the male flower branch parts of Sabina pingii in the initial period to the swelling period of the male flower ball. The agents are sodium abietate water emulsion and / or stone sulfur-mineral oil water emulsion. The dilution multiple of the sodium abietate water emulsion is 50-150 times of liquid, and the dilution multiple of the stone sulfur-mineral oil water emulsion is 150-250 times of liquid. The two kinds of agents can be used in a volume ratio of 1:1-3:1, or can be used alone. Through the dual effects of contact killing and sealing, the epidermal tissue and stoma structure of the male flower ball are damaged, the male flower ball is irreversibly aborted and dried and falls off before the pollen is scattered, and thus the release of pollen is completely inhibited from the source.

Description

Technical Field

[0001] This invention relates to the field of urban landscaping maintenance and source control of allergic diseases, and in particular to a method for source prevention and control of juniper pollen allergy. Background Technology

[0002] Chinese juniper (Sabina chinensis), also known as Chinese juniper, is an evergreen tree belonging to the genus Juniperus of the family Cupressaceae. It can grow up to 20 meters tall and have a diameter at breast height of 3.5 meters. Due to its many advantages such as evergreen foliage, beautiful shape, tolerance to pruning, strong adaptability, and outstanding pollution resistance, it has become a backbone tree species in urban landscaping in northern my country and some southern cities. It is widely planted in parks, streets, courtyards, ancient gardens, and scenic spots, playing an important role in improving the urban ecological environment and creating landscapes.

[0003] However, the Chinese juniper is a typical dioecious plant, with very few being monoecious. Female plants do not produce pollen, while male plants form numerous male flower heads (microsporophyll heads) each spring. Each male flower head has two microsporangia behind the microsporophyll, filled with microspores. Upon maturity, these release a large quantity of lightweight, small pollen grains, with an average diameter of only 24.8 micrometers, making them highly susceptible to wind dispersion and spreading over a wide area. Monitoring shows that tree pollen accounts for 53% of the total pollen in northern Chinese cities during the spring months of March to May. Among these, Chinese juniper pollen is one of the most significant allergens in spring, with its dispersal period typically lasting from mid-March to late April, posing a serious threat to human health.

[0004] Juniper pollen contains various allergenic proteins. Once inhaled by people with allergies, it can easily trigger a series of allergic diseases such as allergic rhinitis, allergic asthma, and allergic conjunctivitis. Symptoms include sneezing, runny nose, nasal congestion, itchy eyes, coughing, and chest tightness. In severe cases, it can lead to acute asthma attacks and even endanger life. With the acceleration of urbanization and the continuous expansion of juniper planting areas, the incidence of juniper pollen allergy has been rising year by year, becoming a significant public health issue affecting the health of urban residents and posing new challenges to urban landscaping and maintenance.

[0005] Currently, the main prevention and control methods for juniper pollen allergy adopt a "passive management" strategy, which involves end-point control during the peak pollen dispersal period. There are two common methods: one is physical sedimentation, which involves spraying water onto the juniper canopy several times a day by water trucks or manually, using the gravity of water to settle the pollen already dispersed in the air and reduce the pollen concentration in the air; the other is pollen fixation, which involves spraying a polymer pollen fixative to try to adhere the dispersed pollen to the surface of branches and leaves and prevent it from spreading further.

[0006] However, these traditional prevention and control methods have obvious defects and shortcomings, making it difficult to fundamentally solve the problem of juniper pollen allergy: First, they only treat the symptoms, not the root cause. The above methods all treat the pollen after it has been released into the air, and cannot prevent the production of allergens (juniper pollen). They can only temporarily reduce the concentration of pollen in the air and cannot eliminate the allergy risk at the source. Moreover, the pollen has a wide dispersion range, and the settling and fixation effects are limited. Second, they are costly, consume a lot of water, and require frequent manual spraying, especially during peak pollen season, when multiple water sprays or fixatives need to be applied daily, which not only increases the maintenance of garden greening. Firstly, it increases costs and wastes precious water resources. Secondly, it can easily cause secondary problems. Frequent watering can keep the surface of juniper leaves in a high-humidity environment for a long time, making them highly susceptible to rust, powdery mildew and other garden diseases, affecting the normal growth of juniper. At the same time, polymer fixatives may remain on the surface of branches and leaves, and long-term use may cause potential damage to the tree. Thirdly, it is significantly affected by the weather. Strong winds and rain can seriously affect the effectiveness of watering and fixatives. When the wind is strong, pollen is dispersed quickly and over a wide area, and the effect of watering on sedimentation is minimal. Rain will wash away the fixatives on the surface of branches and leaves, resulting in a significant reduction in the control effect.

[0007] In addition, some existing technologies have attempted to reduce pollen production by pruning male flowering branches of juniper. However, this method is complex, requires advanced pruning techniques, and excessive pruning can damage the juniper's aesthetic appearance and landscape value. Furthermore, the male flowering branches will regrow after pruning, making long-term control impossible. Additionally, pruning can cause premature pollen dispersal, potentially exacerbating allergy problems. Other studies have attempted to develop pollen-inhibiting agents, but existing agents either have poor control effects or poor safety for the tree, easily causing leaf drop and branch dieback, making large-scale application difficult.

[0008] Therefore, how to inhibit the development and pollen shedding of male juniper flower balls from the source, that is, to replace "end-of-pipe control" with "source control" to achieve long-term and efficient prevention and control of juniper pollen allergy, is a technical problem that urgently needs to be solved in this field, and it is also an urgent need for current urban landscaping maintenance and public health prevention and control work. Summary of the Invention

[0009] To address the shortcomings of existing methods for controlling juniper pollen, such as merely treating the symptoms without addressing the root cause, high costs, susceptibility to secondary problems, and significant weather-related influences, this invention provides a source-based prevention and control method for juniper pollen allergies. Based on the concept of "source control," this method induces irreversible sterility of the male juniper flower bulbs before pollen release by spraying a specific compound agent during the critical development period, completely blocking pollen production and release. This fundamentally eliminates juniper pollen as a source of allergy. It is characterized by high efficiency, environmental friendliness, long-lasting effectiveness, simple operation, low cost, and safety for the tree. It effectively solves many shortcomings of existing control methods, meets the needs of urban landscaping maintenance and source control of allergic diseases, and is suitable for large-scale application.

[0010] To achieve the above-mentioned objectives, the present invention adopts the following technical solution: A method for preventing and controlling juniper pollen allergy at its source includes the following steps: During the early development and swelling stage of male flower balls of Juniperus chinensis, the male flower branches of the Juniperus chinensis plant are precisely located, and a special agent is sprayed using a high-pressure spraying method. The agent consists of sodium rosinate emulsion and lime sulfur mineral oil emulsion. The two agents can be used in combination or sprayed separately. When used in combination, the volume ratio of the two agents is 1:1 to 3:1. Through the synergistic action of contact killing and physical sealing, the agent destroys the epidermal tissue and stomatal structure of the male flower bulb of Juniperus chinensis, inhibits the development and maturation of pollen inside the male flower bulb, and causes irreversible sterility of the male flower bulb of Juniperus chinensis before it splits open and releases pollen, thereby completely inhibiting the release of pollen from the source and reducing the concentration of Juniperus chinensis pollen in the air.

[0011] Furthermore, in the aforementioned agents, the sodium rosinate emulsion is a commercially available product with an effective ingredient content of 30% to 45% and a dilution ratio of 50 to 150 times; the lime sulfur-mineral oil emulsion is a commercially available product with an effective ingredient content of 25% to 35% and a dilution ratio of 150 to 250 times.

[0012] Furthermore, the sodium rosinate emulsion is diluted 100 times, and the lime sulfur-mineral oil emulsion is diluted 200 times.

[0013] Furthermore, the timing of the spraying is the critical period when the scales of the male flower bulbs of Juniperus chinensis loosen and the color changes from dark green to light yellowish-green or light yellow, but before they crack and release pollen. Specifically, it is from late February to mid-March each year, with late February to early March being the preferred time in northern regions and early March to mid-March being the preferred time in southern regions. The timing of the spraying needs to be flexibly adjusted in combination with local meteorological conditions and the development progress of the male flower bulbs of Juniperus chinensis. It can be determined by regularly observing the morphology of the male flower bulbs. The best time to spray is when the diameter of the male flower bulbs reaches 2-3 mm and the scales begin to separate.

[0014] Furthermore, the spraying is performed 1 to 2 times. If there is no rainfall within 24 hours after a single spraying, one spraying is sufficient to achieve the desired control effect. If rainfall occurs within 24 hours after a single spraying and the rainfall exceeds 5 mm, a second spraying is required within 24 hours after the rain stops. The interval between the two sprayings is 5 to 10 days.

[0015] Furthermore, the spraying operation should be carried out on a sunny day with an average daily temperature of 5℃~20℃, no rain, and wind force ≤3, preferably between 9~11 am or 14~16 pm. A high-pressure sprayer should be used during spraying, with the distance between the nozzle and the male flower branch controlled at 30~50cm, and the spraying pressure adjusted to 0.3~0.5MPa to ensure that the pesticide solution evenly covers the surface of the male flower head and the gaps between the scales, so that a thin film of pesticide is formed on the surface of the male flower head without any dripping and waste. During the spraying process, female juniper trees and surrounding sensitive plants should be avoided to prevent accidental spraying of pesticides.

[0016] Furthermore, the sodium pinoresinate emulsion is a plant-derived agent, with sodium pinoresinate as its main component. It has good fat solubility, film-forming properties, and emulsifying properties. It is low in toxicity and leaves no residue, making it safe for humans and animals. Its contact toxicity can destroy the waxy layer of the male flower bulb epidermis, causing the male flower bulb cells to dehydrate and dry out. The lime sulfur-mineral oil emulsion is a mineral-derived agent, composed of lime sulfur compound and light mineral oil. It can form a dense protective film on the surface of the male flower bulb, sealing the stomata and preventing the supply of oxygen and nutrients. At the same time, it also has a bactericidal effect, which can prevent the growth of diseases in the male flower bulb.

[0017] Furthermore, the method is applicable to all areas where male juniper trees are planted, including urban parks, street green spaces, courtyards, ancient buildings and gardens, and scenic spots, especially areas where male juniper trees are concentrated and pollen allergies are prevalent; it can be used for male juniper trees aged 5 to 150 years.

[0018] The beneficial effects of this invention are as follows: 1. Source control for thorough prevention and elimination of allergens: This invention breaks away from the traditional "end-of-pipe control" approach and focuses on "source control." It directly targets the critical development period of juniper male flower heads. Through the dual action of contact killing and blocking, the agent induces irreversible sterility of the male flower heads before pollen release, preventing them from completing the pollen development and release process. This fundamentally eliminates the production of juniper pollen and completely removes the hidden danger of juniper pollen allergies.

[0019] 2. Simple operation, low cost, and large-scale application: The method of this invention is simple and requires no complex equipment or professional technical training. Landscape workers can operate it after simple guidance. It only needs to be sprayed 1-2 times a year during specific window periods, eliminating the need for high-frequency operations during peak pollen season, greatly reducing labor input and water consumption, and lowering the cost of landscape maintenance. The agents used are all commercially available conventional agents, inexpensive and easy to purchase. They are diluted before use, requiring smaller dosages, further reducing control costs. It is suitable for large-scale promotion in various juniper planting areas such as urban parks, street green spaces, courtyards, and ancient gardens, especially suitable for areas with concentrated male juniper trees and high incidence of pollen allergies.

[0020] 3. Environmentally friendly and safe, with no adverse effects on trees and the environment: The sodium rosinate emulsion used in this invention is a plant-derived agent, made from natural rosin and soda ash or caustic soda. It is low in toxicity, leaves no residue, is safe for humans and animals, and is easily degraded in the natural environment, without causing soil or water pollution. The lime sulfur-mineral oil emulsion is a mineral-derived agent, leaves no residue, is safe for natural enemies, and conforms to the concept of green pest control. The two agents, when used in combination, have a strong synergistic effect, effectively inducing male flower bulb abortion while avoiding phytotoxicity to new branches and leaves of the juniper. Multiple trials have verified that after spraying, the juniper grows normally, without leaf drop, dead branches, or other phytotoxicity phenomena, and the trees are vigorous. It is especially suitable for pollen control of ancient juniper trees and will not affect the landscape value or lifespan of the juniper. At the same time, the spraying process can accurately target male flower branches, avoiding accidental spraying of female trees and surrounding sensitive plants, and has no adverse impact on the surrounding ecological environment.

[0021] 4. Dual Mechanism of Action, Long-Lasting Effect, and Low Risk of Resistance: This invention employs a combination of sodium pinoresinate emulsion and lime sulfur-mineral oil emulsion. The two agents have different mechanisms of action and synergistic effects: Sodium pinoresinate has strong contact and corrosive properties, destroying the cuticle and waxy layers of the male flower bulb epidermis, leading to dehydration and drying of the male flower bulb cells. It also has good film-forming properties, helping to seal the stomata of the male flower bulb. Lime sulfur-mineral oil has a physical sealing effect, forming a dense protective film on the surface of the male flower bulb, sealing the stomata, preventing oxygen and nutrient supply, inhibiting pollen development, and also having a bactericidal effect, preventing the growth of diseases in the male flower bulb. This dual mechanism of action completely eliminates the physiological activity of the male flower bulb, resulting in a stable sterilization effect and a long-lasting effect, ensuring that the juniper does not release pollen throughout the pollen season. Furthermore, the unique mechanisms of action of the two agents make it unlikely that long-term use will lead to drug resistance in the male flower bulbs of the juniper, achieving a long-term and stable control effect.

[0022] 5. High adaptability and wide applicability: The method of this invention can flexibly adjust the spraying time and pesticide dilution ratio according to the climate differences between the north and south of my country and the development progress of the male flower balls of Juniperus chinensis. It is applicable to male Juniperus chinensis trees of different regions and ages (5-150 years). Whether it is a large-scale planting area such as urban parks and street green spaces, or a small-scale planting area such as courtyards and ancient gardens, whether it is a common Juniperus chinensis or an ancient Juniperus chinensis, this method can be used for pollen control. It is highly adaptable and has a wide range of applications. Attached Figure Description

[0023] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of the present invention and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0024] Figure 1 This is a flowchart of the prevention and control method of the present invention; Figure 2 This is a flowchart illustrating the pharmaceutical preparation process of the present invention. Detailed Implementation

[0025] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. The components of the embodiments of the present invention described and shown in the accompanying drawings can generally be arranged and designed in various different configurations.

[0026] In the following examples, the sodium rosinate emulsion used was a commercially available product with a 30% active ingredient content, and the lime sulfur-mineral oil emulsion was also a commercially available product with a 30% active ingredient content. Pollen concentration monitoring was performed using a Burkard seven-day spore capacity analyzer, following conventional methods. During monitoring, the sampler was placed in an open area of ​​the monitoring zone, with the air inlet at least 75 cm above surrounding obstacles. Air was drawn in at a rapid rate, and a total air volume of 14.4 m³ was sampled over 24 hours. After staining and slide preparation, pollen counting and species identification were performed under a high-power optical microscope (×400). The pollen concentration was calculated as follows: pollen concentration =Total pollen collected within 24 hours ÷ 14.4m³; The observation of pesticide damage is carried out by visual inspection. The growth of branches and leaves of the juniper plants are observed regularly, and it is recorded whether there are pesticide damage phenomena such as leaf drop, dead branches, and yellowing leaves.

[0027] Example 1: Pollen Source Control Experiment of Juniper in a City Park in Northern China Experimental site: A concentrated planting area of ​​Chinese juniper in a large comprehensive park in a northern city. The area covers a total area of ​​approximately 8,000 square meters and contains 230 Chinese juniper trees, of which 156 are male, accounting for 67.8%. Most of the trees are 50 to 80 years old and are growing well. Every spring from March to April, the pollen from Chinese juniper in this area is severely dispersed, and the incidence of allergic rhinitis and asthma among the surrounding residents is relatively high. It is a high-incidence area for pollen allergies in the area. In the past, the traditional method of spraying water to settle pollen was used for prevention and control, but the effect was not good.

[0028] The experiment took place from February 25 to April 10, 2023, covering the entire process of male juniper bulb development, pollen release and dispersal, with spraying operations taking place on February 28 and March 8.

[0029] Experimental materials: Thirty male Chinese juniper trees with similar growth, an age of about 60 years, and free from pests and diseases were selected and randomly divided into an experimental group, a blank control group, and a traditional method control group, with 15 trees in each group. The experimental group was treated with the method of this invention, the blank control group was left to grow naturally without any treatment, and the traditional method control group was treated with conventional water spraying and sedimentation methods.

[0030] Test method: 1. Experimental Group: The procedure was performed according to the method of this invention. Sodium rosinate emulsion and lime sulfur-mineral oil emulsion were used as the agents, mixed at a volume ratio of 1:1. The sodium rosinate emulsion was diluted 100 times, and the lime sulfur-mineral oil emulsion was diluted 200 times. Clean tap water was used for dilution, and after stirring evenly, the mixture was allowed to stand for 8 minutes to ensure complete emulsification. Spraying was performed on February 28th (during the swelling stage of the male flower bulbs of the juniper, when the scales are slightly open, light yellow, approximately 2.5 mm in diameter, and before cracking and powdering). A backpack high-pressure sprayer was used, with the nozzle 40 cm away from the male flower branch and a spraying pressure of 0.4 MPa. The entire male flower branch was sprayed evenly, ensuring the solution covered the surface of the male flower bulb without dripping. A second spray was performed on March 8th (9 days between the two sprays), with the same spraying conditions and agent ratio as the first spray. No rainfall occurred within 24 hours after the first spray, so no re-spraying was necessary.

[0031] 2. Blank control group: No pesticides or physical treatments were applied; the plants were allowed to grow naturally, and the development of male flower heads and pollen release were observed periodically.

[0032] 3. Control group using traditional methods: During the peak period of juniper pollen dispersal from March 10 to March 25, water spraying was carried out twice a day at 10:00 am and 3:00 pm. Water trucks were used to spray the juniper canopy with water for 30 minutes each time to ensure that the branches and leaves of the canopy were fully moistened and to use the gravity of water to settle the pollen in the air.

[0033] During the experiment, meteorological conditions (temperature, wind force, and rainfall) were recorded daily. The development and pollen release of the three groups of juniper male flower heads were observed and recorded regularly. At the same time, Burkard samplers were used to monitor the concentration of juniper pollen in the air from March 10 to April 5. The monitoring points were set at the center of the area where the three groups of experimental plants were located, with one sampler at each monitoring point, and monitoring was carried out continuously for 26 days. After the experiment, the growth of the three groups of juniper plants was observed, and any pesticide damage was checked.

[0034] Results observation: 1. Male flower head development and pollen release: Seven days after the first spray (March 7th), the male flower heads in the experimental group changed from light yellow to dark brown, and the scales began to dry and curl. Ten days after the second spray (March 18th), more than 95% of the male flower heads were brown and withered, with tightly contracted scales and no cracking, and no pollen release was observed. After the experiment ended (April 10th), all male flower heads were withered, shriveled, and fell off, with no signs of pollen shedding. In the blank control group, the male flower heads developed normally, changing from yellow to brown around March 12th, with the scales gradually cracking. Around March 15th, the peak pollen shedding period began, with yellow, smoky pollen visible to the naked eye. Pollen shedding continued until around April 5th, after which the male flower heads gradually withered. In the traditional method, the male flower heads in the control group developed normally and entered the peak pollen shedding period at the same time as the blank control group. After spraying water every day, the pollen concentration in the air temporarily decreased, but 2-3 hours after spraying water, the pollen concentration returned to a high level, and a large amount of pollen was still scattered. The duration of pollen shedding was basically the same as that of the blank control group.

[0035] 2. Airborne pollen concentration monitoring results: The average pollen concentration in the experimental group was only 6 grains / m³, with a maximum concentration of 12 grains / m³ and a minimum concentration of 2 grains / m³. The average pollen concentration in the blank control group was 2850 grains / m³, with a maximum concentration of 4230 grains / m³ and a minimum concentration of 1560 grains / m³. The average pollen concentration in the traditional method control group was 1240 grains / m³, with a maximum concentration of 1890 grains / m³ and a minimum concentration of 780 grains / m³. Compared with the blank control group, the pollen concentration in the experimental group was reduced by 99.8%; compared with the traditional method control group, the pollen concentration was reduced by 99.5%, demonstrating a significantly better control effect than the traditional method.

[0036] 3. Safety Observation Results: During and after the experiment, the experimental group of juniper plants were observed. New shoots and leaves grew normally, leaves were bright green, and there were no signs of pesticide damage such as leaf drop, withered branches, or yellowing leaves. The trees were in good condition, and there was no significant difference in growth status compared to the blank control group. This indicates that the pesticide used in this invention is safe for juniper plants and has no adverse effects. In contrast, due to frequent watering, three juniper plants in the traditional method control group showed mild rust symptoms, with yellow-brown spots appearing on the leaves, affecting the tree's appearance and growth.

[0037] Example 2: Pollen Source Control Experiment of Ancient Juniper Trees in a Scenic Area in Southern China Experimental location: A group of ancient juniper trees in a scenic spot in southern China. The group consists of 42 ancient juniper trees, with an age of about 100 to 120 years, of which 28 are male, accounting for 66.7%. Every spring, the scenic area receives a large number of tourists. During the period when juniper pollen is dispersed, there are many complaints from tourists about allergy symptoms such as itchy eyes, sneezing, and coughing, which seriously affects the tourist experience. In addition, due to the protection requirements of ancient trees, it is not possible to use methods such as pruning or high-frequency water spraying for prevention and control. In the past, there have been no effective pollen control measures.

[0038] Experiment period: March 1 to April 5, 2024. Considering the climate characteristics of southern China, where the male flower balls of Juniperus chinensis develop earlier, the spraying operation was carried out on March 2.

[0039] Experimental materials: Twenty male Chinese juniper trees of about 110 years old with similar growth, free from pests and diseases, were selected and randomly divided into an experimental group and a blank control group, with 10 trees in each group. The experimental group was treated with the method of this invention, while the blank control group was left to grow naturally without any treatment.

[0040] Test method: 1. Experimental Group: Considering the needs of ancient tree protection, the preferred scheme of this invention was adopted. The agents used were sodium rosinate emulsion and lime sulfur-mineral oil emulsion, mixed at a volume ratio of 1:1. The sodium rosinate emulsion was diluted 120 times, and the lime sulfur-mineral oil emulsion was diluted 220 times. Clean tap water was used for dilution, and after stirring evenly, the mixture was allowed to stand for 10 minutes to ensure full emulsification. Spraying was performed on March 2nd (the male flower clusters of the Chinese juniper are in the mid-to-late development stage, with loose scales, a light yellowish-green color, approximately 3mm in diameter, and before cracking and powdering). A backpack high-pressure sprayer was used, with the spraying pressure controlled below 0.3MPa. The nozzle was 50cm away from the male flower branches, focusing on spraying the male flower branches in the lower and middle parts of the crown to avoid direct spraying of the trunk and bark, ensuring even coverage of the male flower cluster surface without dripping or wasting. If there was no rainfall within 3 days after spraying, a second spray was unnecessary.

[0041] 2. Blank control group: No pesticides or physical treatments were applied. The trees were allowed to grow naturally, and the development of male flower heads and pollen release, as well as the growth status of the ancient trees, were observed regularly by professionals specializing in the protection of ancient trees.

[0042] During the experiment, weather conditions were recorded daily, and the development of male flower balls and pollen release of the two groups of ancient juniper trees were observed and recorded regularly. At the same time, tourist complaint records from the scenic area management office were collected, and the number of complaints from tourists about pollen allergy symptoms was counted. After the experiment, ancient tree protection experts were invited to evaluate the growth status of the two groups of ancient juniper trees and check whether there was any pesticide damage.

[0043] Results observation: 1. Male flower head development and pollen release: Observations were conducted on the experimental group 15 days after spraying (March 17). The male flower heads in the sprayed area had a shriveling rate of over 92%, turned dark brown, and the scales were shrunken and without cracking. No fresh pollen was observed being released. After the experiment ended (April 5), all male flower heads had dried up and fallen off, with no signs of pollen release. In the blank control group, the male flower heads of the ancient juniper trees developed normally, entering the peak pollen release period around March 18. The scales cracked extensively, and pollen was dispersed by the wind. Obvious yellow pollen deposits were visible on the ground in the scenic area, and pollen release continued until around April 2.

[0044] 2. Tourist Complaint Feedback: According to statistics from the scenic area management office, in the experimental group area, there were 3 complaints from tourists about pollen allergy symptoms such as itchy eyes, sneezing, and coughing during the experiment, which is about 85% less than the same period in previous years (20 cases); in the blank control group area, there were 18 complaints from tourists about allergies, which is basically the same as the same period in previous years. This shows that the method of the present invention effectively reduces the dispersion of juniper pollen and improves the tourist experience.

[0045] 3. Safety assessment results of ancient trees: On-site assessment by ancient tree protection experts concluded that after spraying the ancient juniper trees in the experimental group with the agent, the bark and main branches were intact, the new branches and leaves grew normally, and there were no signs of pesticide damage such as leaf drop, dead branches, or bark cracking. The trees were stable and showed no difference in growth status compared with the blank control group. This indicates that the method of the present invention is safe for ancient juniper trees, will not cause damage to the ancient trees, and meets the requirements for ancient tree protection.

[0046] in conclusion As can be seen from Examples 1 and 2 above, the present invention, by spraying a compound agent of sodium rosinate emulsion and lime sulfur-mineral oil emulsion in a specific ratio during the critical development period of juniper male flower heads (from the early development stage to the swelling stage), can effectively induce irreversible sterility of juniper male flower heads before pollen shedding, thus preventing them from flowering and shedding pollen. This reduces the concentration of juniper pollen in the air by more than 99%, which is significantly better than the control effect of traditional multiple daily water spraying. It can also effectively reduce pollen allergy complaints and improve the living environment and visitor experience.

[0047] This method is simple to operate, low in cost, and the pesticides used are environmentally friendly, safe, and residue-free. It has no adverse effects on juniper plants (including ancient junipers). Its dual mechanism of action makes it long-lasting and less prone to developing resistance. The timing of spraying and the dilution ratio of the pesticide can be flexibly adjusted according to the differences in climate between the north and south. It is suitable for large-scale promotion and application in various juniper planting areas such as urban parks, street green spaces, courtyards, ancient buildings and gardens, and scenic spots. It has extremely high practical value and promotion prospects. It can effectively solve the problem of controlling juniper pollen allergy, while taking into account both the value of urban landscaping and the health needs of residents.

[0048] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A method for preventing and controlling juniper pollen allergy at its source, characterized in that, Includes the following steps: During the early development and swelling stage of male flower balls of Juniperus chinensis, the male flower branches of the Juniperus chinensis plant are precisely located, and a special agent is sprayed using a high-pressure spraying method. The agent consists of sodium rosinate emulsion and lime sulfur mineral oil emulsion. The two agents can be used in combination or sprayed separately. When used in combination, the volume ratio of the two agents is 1:1 to 3:

1. Through the synergistic action of contact killing and physical sealing, the agent destroys the epidermal tissue and stomatal structure of the male flower bulb of Juniperus chinensis, inhibits the development and maturation of pollen inside the male flower bulb, and causes irreversible sterility of the male flower bulb of Juniperus chinensis before it splits open and releases pollen, thereby completely inhibiting the release of pollen from the source and reducing the concentration of Juniperus chinensis pollen in the air.

2. The method for preventing and controlling juniper pollen allergy according to claim 1, characterized in that, In the aforementioned agents, the sodium rosinate emulsion is a commercially available product with an effective ingredient content of 30% to 45% and a dilution ratio of 50 to 150 times; the lime sulfur-mineral oil emulsion is a commercially available product with an effective ingredient content of 25% to 35% and a dilution ratio of 150 to 250 times.

3. The method for preventing and controlling juniper pollen allergy according to claim 2, characterized in that, The sodium rosinate emulsion is diluted 100 times, and the lime sulfur-mineral oil emulsion is diluted 200 times.

4. The method for preventing and controlling juniper pollen allergy according to claim 1, characterized in that, The optimal time for spraying is during the critical period when the scales of the male juniper bulbs loosen and their color changes from dark green to light yellowish-green or light yellow, but before they crack and release pollen. Specifically, this is from late February to mid-March each year, with late February to early March being the preferred time in northern regions and early March to mid-March in southern regions. The timing of spraying needs to be flexibly adjusted based on local weather conditions and the development progress of the male juniper bulbs. It can be determined by regularly observing the morphology of the male bulbs. The best time to spray is when the diameter of the male bulbs reaches 2-3 mm and the scales begin to separate.

5. The method for preventing and controlling juniper pollen allergy according to claim 1, characterized in that, The spraying should be done 1 to 2 times. If there is no rainfall within 24 hours after a single spraying, one spraying is sufficient to achieve the desired control effect. If there is rainfall within 24 hours after a single spraying and the rainfall exceeds 5 mm, a second spraying is required within 24 hours after the rain stops. The interval between the two sprayings should be 5 to 10 days.

6. The method for preventing and controlling juniper pollen allergy according to claim 1, characterized in that, When performing the spraying operation, choose a sunny day with an average daily temperature of 5℃~20℃, no rain, and wind force ≤3. It is preferable to spray between 9~11 am or 14~16 pm. Use a high-pressure sprayer, and control the distance between the nozzle and the male flower branch to be 30~50cm. Adjust the spraying pressure to 0.3~0.5MPa to ensure that the liquid evenly covers the surface of the male flower head and the gaps between the scales. It is best to form a thin film of liquid on the surface of the male flower head without any dripping and waste. During the spraying process, avoid female juniper trees and surrounding sensitive plants to avoid accidental spraying and adverse effects.

7. The method for preventing and controlling juniper pollen allergy according to claim 1, characterized in that, The sodium pinoresinate emulsion is a plant-derived agent, with sodium pinoresinate as its main component. It has good fat solubility, film-forming properties, and emulsifying properties. It is low in toxicity and leaves no residue, making it safe for humans and animals. Its contact toxicity can destroy the waxy layer of the male flower bulb epidermis, causing the male flower bulb cells to dehydrate and dry out. The lime sulfur-mineral oil emulsion is a mineral-derived agent, composed of lime sulfur compound and light mineral oil. It can form a dense protective film on the surface of the male flower bulb, sealing the stomata and preventing the supply of oxygen and nutrients. At the same time, it also has a bactericidal effect, which can prevent the growth of diseases in the male flower bulb.

8. The method for preventing and controlling juniper pollen allergy according to claim 1, characterized in that, The method is applicable to all areas where male juniper trees are planted, including urban parks, street green spaces, courtyards, ancient buildings and gardens, and scenic spots. It is especially suitable for areas where male juniper trees are concentrated and pollen allergies are prevalent. It can be used for male juniper trees aged 5 to 150 years.

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