Livestock and poultry nutrition regulation type feed additive based on angelica sinensis composite fermentation

Abstract

This invention discloses a livestock and poultry nutrition-regulating feed additive based on Angelica sinensis compound fermentation. The feed additive raw materials, by weight, include the following components: 35 parts Angelica sinensis, 22 parts American ginseng, 18 parts Cistanche deserticola, 15 parts Polygonatum sibiricum, and 10 parts Ziziphus jujuba var. spinosa. The preparation method includes the following steps: 1. Weigh the raw materials according to the weight ratio, remove impurities and clean to obtain a mixed powder; 2. Add the mixed powder to distilled water, filter to remove residue, and obtain an aqueous extract; 3. Add a compound enzyme to the aqueous extract, and after enzymatic hydrolysis, heat to obtain an enzymatic hydrolysate; 4. Inoculate the enzymatic hydrolysate with compound probiotics, and after liquid fermentation, obtain a fermentation broth; 5. Concentrate the fermentation broth under reduced pressure to obtain a concentrated liquid; 6. Mix the concentrated liquid with a carrier, dry and pulverize to obtain the finished livestock and poultry nutrition-regulating feed additive. The advantages of this invention are: through specific raw material compatibility and compound fermentation process, it improves the content and bioavailability of active ingredients, and enhances the comprehensive regulation of livestock and poultry immunity and nutrition.

Description

Technical Field

[0001] This invention relates to the field of feed additive technology, specifically to a livestock and poultry nutrition-regulating feed additive based on the compound fermentation of Angelica sinensis. Background Technology

[0002] In current livestock farming practices, problems such as low immunity and frequent stress responses in livestock and poultry are common. Traditional feed additives often rely on antibiotics or single nutritional supplements, and long-term use can easily lead to drug residues, increased drug resistance, and affect the safety of livestock and poultry.

[0003] Natural plant-based ingredients, with their wide availability, high safety, and combined nutritional and conditioning functions, are gradually becoming the core direction of livestock and poultry feed additive research and development. Among them, medicinal and edible ingredients such as angelica, American ginseng, cistanche, polygonatum, and jujube seed are rich in active ingredients such as polysaccharides, saponins, and flavonoids, and have the effects of replenishing qi and blood, regulating immunity, resisting stress, and improving intestinal function, showing great potential in the field of livestock and poultry nutritional regulation. Summary of the Invention

[0004] The technical problem to be solved by this invention is to overcome the above-mentioned technical defects and provide a livestock and poultry nutrition regulation feed additive based on Angelica sinensis compound fermentation. Through specific raw material compatibility and compound fermentation process, the content and bioavailability of active ingredients are improved, thereby enhancing the comprehensive regulation of livestock and poultry immunity and nutrition.

[0005] To solve the above problems, the technical solution of the present invention is a livestock and poultry nutrition-regulating feed additive based on Angelica sinensis compound fermentation. The feed additive raw materials include the following components by weight: 35 parts Angelica sinensis, 22 parts American ginseng, 18 parts Cistanche deserticola, 15 parts Polygonatum sibiricum, and 10 parts Ziziphus jujuba var. spinosa.

[0006] This invention also discloses a method for preparing a livestock and poultry nutrition-regulating feed additive based on Angelica sinensis compound fermentation, comprising the following steps: Step 1: Raw material pretreatment: Weigh out Angelica sinensis, American ginseng, Cistanche deserticola, Polygonatum sibiricum, and Ziziphus jujuba var. spinosa according to the weight ratio, remove impurities and clean them to obtain a mixed powder; Step 2, Water extraction: Add the mixed powder to distilled water, filter to remove residue, and obtain water extract; Step 3, compound enzymatic hydrolysis: Add compound enzyme to the aqueous extract and hydrolyze for 2-3 hours at pH 5.5-6.5 and temperature 45-55℃. After hydrolysis, raise the temperature to obtain the hydrolysate. Step 4: Probiotic liquid fermentation: Inoculate compound probiotics into the enzymatic hydrolysate at a volume of 3%-5% of the hydrolysate. After liquid fermentation, the fermentation broth is obtained. Step 5: Concentration and Drying: The fermentation broth is concentrated under reduced pressure to obtain a concentrated liquid; Step 6, Carrier Adsorption and Compounding: Mix the concentrate and carrier at a weight ratio of 1:0.75-1.5 to ensure that the active ingredients in the concentrate are uniformly adsorbed onto the carrier surface. After drying and pulverizing, the finished product of livestock and poultry nutrition regulating feed additive is obtained.

[0007] Preferably, in step one, the raw material is pulverized to 40-60 mesh.

[0008] Preferably, in step two, the water treatment temperature is 80-90℃.

[0009] Preferably, in step three, the complex enzyme includes one or more of cellulase, pectinase, and protease, and after enzymatic hydrolysis, the temperature is raised to 85-90℃ and maintained for 10-15 minutes.

[0010] Preferably, in step four, the compound probiotics include one or more of Lactobacillus acidophilus, Bacillus subtilis, and Saccharomyces cerevisiae, and the fermentation is adjusted to a temperature of 30-37℃, a pH of 6.0-7.0, and a time of 48-72 hours.

[0011] Preferably, in step five, the reduced pressure concentration temperature is 50-60℃ and the vacuum degree is 0.08-0.1MPa.

[0012] Preferably, in step six, the mixing temperature is 25-35℃, the stirring and adsorption are carried out for 30-60 minutes, and the mixture is pulverized to 80-100 mesh.

[0013] The advantages of this invention compared to existing technologies are: 1. This invention uses Angelica sinensis as the core ingredient, combined with American ginseng, Cistanche deserticola, Polygonatum sibiricum, and Ziziphus jujuba var. spinosa. Angelica sinensis replenishes qi and blood, American ginseng replenishes qi and yin, Polygonatum sibiricum replenishes qi and yin, and Cistanche deserticola tonifies the kidneys and benefits essence, thus ensuring the kidney health and intestinal function of livestock and poultry. Ziziphus jujuba var. spinosa has anti-stress properties and relieves stress reactions in livestock and poultry. The synergistic effect of each ingredient can achieve the comprehensive effects of replenishing qi and blood, improving immunity, and resisting stress.

[0014] 2. This invention employs a process of water extraction and compound enzymatic hydrolysis, followed by probiotic liquid fermentation, concentration, and adsorption and compounding with a carrier. This process improves the extraction rate of active ingredients in the raw materials, enhances the biological activity of the active ingredients, and solves the problems of low utilization rate of active ingredients and insufficient efficacy in traditional processes. Attached Figure Description

[0015] Figure 1 This is a process flow diagram of the present invention. Detailed Implementation

[0016] To make the content of this invention easier to understand, the technical solutions in the embodiments of this invention will be clearly and completely described below with reference to the accompanying drawings.

[0017] like Figure 1 As shown, the livestock and poultry nutrition-regulating feed additive based on Angelica sinensis compound fermentation includes the following ingredients by weight: 35 parts Angelica sinensis, 22 parts American ginseng, 18 parts Cistanche deserticola, 15 parts Polygonatum sibiricum, and 10 parts Ziziphus jujuba var. spinosa.

[0018] The preparation method of a livestock and poultry nutrition-regulating feed additive based on Angelica sinensis compound fermentation includes the following steps: Step 1: Raw material pretreatment: Weigh out Angelica sinensis, American ginseng, Cistanche deserticola, Polygonatum sibiricum, and Ziziphus jujuba var. spinosa according to the weight ratio, remove impurities and clean them to obtain a mixed powder; grind the raw materials to 40-60 mesh.

[0019] Step 2, Water extraction: Add the mixed powder to distilled water, filter to remove residue, and obtain water extract; water treatment temperature 80-90℃.

[0020] Step 3, compound enzymatic hydrolysis: Add compound enzyme to the aqueous extract and hydrolyze for 2-3 hours at pH 5.5-6.5 and temperature 45-55℃. After hydrolysis, raise the temperature to obtain the hydrolysate. The compound enzyme includes one or more of cellulase, pectinase, and protease. After hydrolysis, raise the temperature to 85-90℃ and hold for 10-15 minutes.

[0021] Step 4, Probiotic Liquid Fermentation: Inoculate the enzymatic hydrolysate with compound probiotics at a concentration of 3%-5% of the hydrolysate. After liquid fermentation, the fermentation broth is obtained. The compound probiotics include one or more of Lactobacillus acidophilus, Bacillus subtilis, and Saccharomyces cerevisiae. The fermentation is adjusted to a temperature of 30-37℃, a pH of 6.0-7.0, and a time of 48-72 hours.

[0022] Step 5: Concentration and Drying: The fermentation broth is concentrated under reduced pressure to obtain a concentrated solution; the concentration temperature is 50-60℃ and the vacuum degree is 0.08-0.1MPa.

[0023] Step Six: Carrier Adsorption and Compounding: Mix the concentrated liquid and the carrier at a weight ratio of 1:0.75-1.5 to ensure that the active ingredients in the concentrated liquid are uniformly adsorbed onto the carrier surface. After drying and pulverizing, the finished livestock and poultry nutrition-regulating feed additive is obtained. The mixing temperature is 25-35℃, and the mixture is stirred and adsorbed for 30-60 minutes. The mixture is then pulverized to 80-100 mesh. Example 1

[0024] The preparation method of a livestock and poultry nutrition-regulating feed additive based on Angelica sinensis compound fermentation includes the following steps: Step 1: Raw material pretreatment: Weigh 35g of Angelica sinensis, 15g of Polygonatum sibiricum, 18g of Cistanche deserticola, 10g of Ziziphus jujuba var. spinosa, and 22g of Panax quinquefolius. After removing impurities and washing, grind them separately to 50 mesh and mix them evenly to obtain mixed raw material powder.

[0025] Step 2, Water extraction: Add the mixed raw material powder to distilled water and extract twice at 85°C for 2 hours each time. Combine the two extracts and filter through a 200-mesh filter cloth to remove the residue and obtain the water extract.

[0026] Step 3, compound enzymatic hydrolysis: Add a compound enzyme consisting of cellulase, pectinase and protease to the aqueous extract. The amount of compound enzyme added is 0.5% of the mass of the aqueous extract. Adjust the pH to 6.0 and enzymatically hydrolyze at 50℃ for 2.5 hours. After the enzymatic hydrolysis is completed, raise the temperature to 85℃ for 15 minutes to inactivate the enzyme and obtain the enzymatic hydrolysate.

[0027] Step 4: Probiotic liquid fermentation: Inoculate the enzymatic hydrolysate with a compound probiotic consisting of Lactobacillus acidophilus, Bacillus subtilis, and Saccharomyces cerevisiae in proportion to the number of live bacteria. The inoculation amount is 4% of the volume of the enzymatic hydrolysate. Ferment in liquid form for 60 hours at 35°C and pH 6.5. After fermentation, the fermentation liquid is obtained.

[0028] Step 5: Concentration and Drying: The fermentation broth is concentrated under reduced pressure at 55℃ and a vacuum of 0.09MPa to obtain a concentrated solution.

[0029] Step 6: Carrier adsorption and compounding: Weigh 40 kg of corn cob powder as a carrier, mix the concentrated liquid and corn cob powder at a weight ratio of 1:1, stir and adsorb at 30℃ for 45 minutes, then transfer to a vacuum drying oven and dry at 55℃ until the moisture content is ≤10%, and pulverize to 90 mesh to obtain the finished product of livestock and poultry nutrition regulating feed additive. Example 2

[0030] The preparation method of a livestock and poultry nutrition-regulating feed additive based on Angelica sinensis compound fermentation includes the following steps: Step 1: Raw material pretreatment: Weigh 35g of Angelica sinensis, 15g of Polygonatum sibiricum, 18g of Cistanche deserticola, 10g of Ziziphus jujuba var. spinosa, and 22g of Panax quinquefolius. After removing impurities and washing, grind them separately to 40 mesh and mix them evenly to obtain mixed raw material powder.

[0031] Step 2, Water extraction: Add the mixed raw material powder to distilled water and extract twice at 80℃, each time for 2 hours. Combine the two extracts and filter through a 200-mesh filter cloth to remove the residue and obtain the water extract.

[0032] Step 3, compound enzymatic hydrolysis: Add a compound enzyme consisting of cellulase, pectinase and protease to the aqueous extract. The amount of compound enzyme added is 0.5% of the mass of the aqueous extract. Adjust the pH to 5.5 and enzymatically hydrolyze at 45℃ for 2 hours. After the enzymatic hydrolysis is completed, raise the temperature to 90℃ for 110 minutes to inactivate the enzyme and obtain the enzymatic hydrolysate.

[0033] Step 4: Probiotic liquid fermentation: Inoculate the enzymatic hydrolysate with a compound probiotic consisting of Lactobacillus acidophilus, Bacillus subtilis, and Saccharomyces cerevisiae in proportion to the number of live bacteria. The inoculation amount is 4% of the volume of the enzymatic hydrolysate. Ferment in liquid form for 48 hours at 30°C and pH 6.0. After fermentation, the fermentation liquid is obtained.

[0034] Step 5: Concentration and Drying: The fermentation broth is concentrated under reduced pressure at 50°C and a vacuum of 0.08 MPa to obtain a concentrated solution.

[0035] Step 6: Carrier adsorption and compounding: Weigh 40 kg of corn cob powder as a carrier, mix the concentrated liquid with the corn cob powder at a weight ratio of 1:1, stir and adsorb at 25℃ for 30 minutes, then transfer to a vacuum drying oven and dry at 55℃ until the moisture content is ≤10%, and pulverize to 80 mesh to obtain the finished product of livestock and poultry nutrition regulating feed additive. Example 3

[0036] The preparation method of a livestock and poultry nutrition-regulating feed additive based on Angelica sinensis compound fermentation includes the following steps: Step 1: Raw material pretreatment: Weigh 35g of Angelica sinensis, 15g of Polygonatum sibiricum, 18g of Cistanche deserticola, 10g of Ziziphus jujuba var. spinosa, and 22g of Panax quinquefolius. After removing impurities and washing, grind them separately to 60 mesh and mix them evenly to obtain mixed raw material powder.

[0037] Step 2, Water extraction: Add the mixed raw material powder to distilled water and extract twice at 90℃, each time for 2 hours. Combine the two extracts and filter through a 200-mesh filter cloth to remove the residue and obtain the water extract.

[0038] Step 3: Compound enzymatic hydrolysis: Add a compound enzyme consisting of cellulase, pectinase and protease to the aqueous extract. The amount of compound enzyme added is 0.5% of the mass of the aqueous extract. Adjust the pH to 6.5 and enzymatically hydrolyze at 55℃ for 3 hours. After the enzymatic hydrolysis is completed, raise the temperature to 90℃ for 15 minutes to inactivate the enzyme and obtain the enzymatic hydrolysate.

[0039] Step 4: Probiotic liquid fermentation: Inoculate the enzymatic hydrolysate with a compound probiotic consisting of Lactobacillus acidophilus, Bacillus subtilis, and Saccharomyces cerevisiae in proportion to the number of live bacteria. The inoculation amount is 4% of the volume of the enzymatic hydrolysate. Ferment in liquid form for 72 hours at 37°C and pH 7.0. After fermentation, the fermentation liquid is obtained.

[0040] Step 5: Concentration and Drying: The fermentation broth is concentrated under reduced pressure at 60℃ and a vacuum of 0.1MPa to obtain a concentrated solution.

[0041] Step 6: Carrier adsorption and compounding: Weigh 40 kg of corn cob powder as a carrier, mix the concentrated liquid with the corn cob powder at a weight ratio of 1:1, stir and adsorb at 35℃ for 60 minutes, then transfer to a vacuum drying oven and dry at 55℃ until the moisture content is ≤10%, and pulverize to 100 mesh to obtain the finished product of livestock and poultry nutrition regulating feed additive.

[0042] The present invention and its embodiments have been described above, and such description is not restrictive. In conclusion, if those skilled in the art are inspired by this description and design similar structures and embodiments without departing from the spirit of the invention, such designs should fall within the protection scope of the present invention.

Claims

1. A livestock and poultry nutrition-regulating feed additive based on Angelica sinensis compound fermentation, characterized in that, The feed additive ingredients, by weight, include the following components: 35 parts Angelica sinensis, 22 parts American ginseng, 18 parts Cistanche deserticola, 15 parts Polygonatum sibiricum, and 10 parts Ziziphus jujuba var. spinosa.

2. The preparation method of the livestock and poultry nutrition-regulating feed additive based on Angelica sinensis compound fermentation according to claim 1, characterized in that, Includes the following steps: Step 1: Raw material pretreatment: Weigh out Angelica sinensis, American ginseng, Cistanche deserticola, Polygonatum sibiricum, and Ziziphus jujuba var. spinosa according to the weight ratio, remove impurities and clean them to obtain a mixed powder; Step 2, Water extraction: Add the mixed powder to distilled water, filter to remove residue, and obtain water extract; Step 3, compound enzymatic hydrolysis: Add compound enzyme to the aqueous extract and hydrolyze for 2-3 hours at pH 5.5-6.5 and temperature 45-55℃. After hydrolysis, raise the temperature to obtain the hydrolysate. Step 4: Probiotic liquid fermentation: Inoculate compound probiotics into the enzymatic hydrolysate at a volume of 3%-5% of the hydrolysate. After liquid fermentation, the fermentation broth is obtained. Step 5: Concentration and Drying: The fermentation broth is concentrated under reduced pressure to obtain a concentrated liquid; Step 6, Carrier Adsorption and Compounding: Mix the concentrate and carrier at a weight ratio of 1:0.75-1.5 to ensure that the active ingredients in the concentrate are uniformly adsorbed onto the carrier surface. After drying and pulverizing, the finished product of livestock and poultry nutrition regulating feed additive is obtained.

3. The livestock and poultry nutrition-regulating feed additive based on Angelica sinensis compound fermentation according to claim 2, characterized in that: In step one, the raw materials are pulverized to 40-60 mesh.

4. The livestock and poultry nutrition-regulating feed additive based on Angelica sinensis compound fermentation according to claim 2, characterized in that: In step two, the water treatment temperature is 80-90℃.

5. The livestock and poultry nutrition-regulating feed additive based on Angelica sinensis compound fermentation according to claim 2, characterized in that: In step three, the complex enzyme includes one or more of cellulase, pectinase, and protease. After enzymatic hydrolysis, the temperature is raised to 85-90℃ and maintained for 10-15 minutes.

6. The livestock and poultry nutrition regulating feed additive based on Angelica sinensis compound fermentation according to claim 2, characterized in that: In step four, the compound probiotics include one or more of Lactobacillus acidophilus, Bacillus subtilis, and Saccharomyces cerevisiae, and the fermentation is adjusted to a temperature of 30-37℃, a pH of 6.0-7.0, and a time of 48-72 hours.

7. The livestock and poultry nutrition-regulating feed additive based on Angelica sinensis compound fermentation according to claim 2, characterized in that: In step five, the reduced pressure concentration temperature is 50-60℃ and the vacuum degree is 0.08-0.1MPa.

8. The livestock and poultry nutrition-regulating feed additive based on Angelica sinensis compound fermentation according to claim 2, characterized in that: In step six, the mixing temperature is 25-35℃, the stirring and adsorption is carried out for 30-60 minutes, and the mixture is pulverized to 80-100 mesh.

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